Rroxscaffold_2G00087660

carboxylesterase 17

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0009064
Physical Location & Seq
Forward (+)
9662111 .. 9663121
1011 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00087660.1

Sequence Viewer

Length: 1011 bp
ATGGCAACCATTTCCTTCGATCCAAGGCCTAATCTTCAAGCAACTAACAACCATCAACATGGAGGTGTTGTTGAAGAAATTGAAGGCCTTATCAGAGTCCACAAAAATGGATACATAGAAAGGCCTTCAATTATCCCTAGTGTCCCTTGCACTACTGGAGCAGCTAGAGATGTTGTCATTGACAAATTCACCAACTTGTGGGCGCGCATTTATGTTCCCAGCCATCCCGCCGCCAACAACCTCCCGGTGCTTGTTTACTTTCATGGTGGAGGATTCTGTGTTGGCTCTGCTGCTTGGAGCTGCTACCATGAGTTCCTCAGCAACCTTGCTTGTAAAGCAAGTTGCGTCATCGTCTCTGTTAATTACCGTTTAGCCCCTGAGAACTGTCTCCCTGCTGCCTATGATGATGGTGTCAACGCTGTTATGTGGGTGAAACAACAAGCTCTCAGTCAGAGTTGCGAGCAAAAATGGTGGTTAAGTCGGTGCAACCTCTCGAGCTTGTTCATAAGTGGTGACAGTGCAGGGGCTAACATAGCCTACAATGTCACCACCCGGTTATCAAATGAACACTCGGTTTTAAAACCACTGCGTCTCAACGGTACTGTTTTGATCCAACCTTTCTTTGGAGGTGAAGCGCGTACTTGGTCTGAAAAATATGCAACTCAGCCGCCACAGTCTGCACTAACTCTATCAAACTCCGATGTGTATTGGCGATTGTCACTGCCGTTGGGGGCCAACCGAGACCATCCGTGGTGCAATCCCTTAGCGAATGGTGTGGCCAAGTTGAGGGACTTGAGAATCCCAGCTATAATGGTGTGCGTATCAGAGTTGGATATATTGAAAGATAGGAATTTGGAGCTGTGTAATGCTTTGACTAGCTTGGGAAAAAAGGTGGAAACAGTAATGTACAAAGGGGTTGGGCATGCATTTCAAGTTCTGCATAATTCTCAGCTCTCTCATCCTAGGACCCAAGAACTAGTGTCCCACATCAAGGCTTTCATAAACCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

336

Amino Acids

37.12

Weight (kDa)

8.45

Isoelectric Point (pI)

40.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BD-FAE PF20434 70 - 183 3.4e-12 BD-FAE
COesterase PF00135 70 - 126 4.4e-10 Carboxylesterase family
Abhydrolase_3 PF07859 84 - 311 1.9e-58 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 198
AccII CGCG 2 cut(s) 205, 637
AciI CCGC 3 cut(s) 228, 231, 668
AclWI GGATC 2 cut(s) 14, 604
AcoI YGGCCR 1 cut(s) 777
AcsI RAATTY 2 cut(s) 185, 850
AfaI GTAC 3 cut(s) 601, 640, 908
AfiI CCNNNNNNNGG 4 cut(s) 198, 623, 786, 991
AgsI TTSAA 6 cut(s) 38, 74, 83, 129, 841, 932
AhlI ACTAGT 1 cut(s) 976
AluBI AGCT 8 cut(s) 164, 300, 443, 498, 806, 859, 879, 952
AluI AGCT 8 cut(s) 164, 300, 443, 498, 806, 859, 879, 952
Alw26I GTCTC 4 cut(s) 358, 392, 596, 735
AlwI GGATC 2 cut(s) 14, 604
Ama87I CYCGRG 1 cut(s) 493
AoxI GGCC 5 cut(s) 26, 85, 122, 732, 777
ApeKI GCWGC 4 cut(s) 161, 290, 300, 395
ApoI RAATTY 2 cut(s) 185, 850
AspA2I CCTAGG 1 cut(s) 962
AspLEI GCGC 3 cut(s) 205, 207, 637
AspS9I GGNCC 2 cut(s) 732, 966
AsuC2I CCSGG 2 cut(s) 245, 553
AsuHPI GGTGA 5 cut(s) 181, 442, 524, 538, 641
AvaI CYCGRG 1 cut(s) 493
AvaII GGWCC 1 cut(s) 966
AvrII CCTAGG 1 cut(s) 962
BalI TGGCCA 1 cut(s) 779
BbvCI CCTCAGC 1 cut(s) 317
BbvI GCAGC 4 cut(s) 173, 277, 287, 382
BccI CCATC 4 cut(s) 60, 231, 401, 753
BceAI ACGGC 1 cut(s) 709
BciVI GTATCC 1 cut(s) 104
BcnI CCSGG 2 cut(s) 245, 553
BcoDI GTCTC 4 cut(s) 358, 392, 596, 735
BcuI ACTAGT 1 cut(s) 976
BfaI CTAG 5 cut(s) 138, 165, 876, 963, 977
BfuI GTATCC 1 cut(s) 104
BisI GCNGC 6 cut(s) 162, 231, 291, 301, 396, 668
BlnI CCTAGG 1 cut(s) 962
BlsI GCNGC 6 cut(s) 163, 232, 292, 302, 397, 669
Bme1390I CCNGG 2 cut(s) 245, 553
Bme18I GGWCC 1 cut(s) 966
BmeT110I CYCGRG 1 cut(s) 493
BmgT120I GGNCC 2 cut(s) 732, 966
BmiI GGNNCC 2 cut(s) 733, 968
BmrFI CCNGG 2 cut(s) 245, 553
BpmI CTGGAG 1 cut(s) 177
Bpu10I CCTNAGC 2 cut(s) 317, 763
BpuEI CTTGAG 1 cut(s) 814
BpuMI CCSGG 2 cut(s) 245, 553
BsaI GGTCTC 1 cut(s) 735
BsaJI CCNNGG 3 cut(s) 23, 749, 962
BsaXI ACNNNNNCTCC 2 cut(s) 54, 84
Bsc4I CCNNNNNNNGG 4 cut(s) 198, 623, 786, 991
Bse1I ACTGG 2 cut(s) 160, 1006
BseDI CCNNGG 3 cut(s) 23, 749, 962
BseGI GGATG 3 cut(s) 223, 745, 958
BseLI CCNNNNNNNGG 4 cut(s) 198, 623, 786, 991
BseMII CTCAG 5 cut(s) 331, 369, 460, 677, 962
BseNI ACTGG 2 cut(s) 160, 1006
BsePI GCGCGC 1 cut(s) 203
BseXI GCAGC 4 cut(s) 173, 277, 287, 382
BseYI CCCAGC 2 cut(s) 218, 802
BsgI GTGCAG 2 cut(s) 540, 663
Bsh1236I CGCG 2 cut(s) 205, 637
BshFI GGCC 5 cut(s) 28, 87, 124, 734, 779
BsiHKCI CYCGRG 1 cut(s) 493
BsiSI CCGG 2 cut(s) 245, 553
BslFI GGGAC 3 cut(s) 128, 803, 967
BslI CCNNNNNNNGG 4 cut(s) 198, 623, 786, 991
BsmAI GTCTC 4 cut(s) 358, 392, 596, 735
BsmBI CGTCTC 2 cut(s) 358, 596
BsmFI GGGAC 3 cut(s) 128, 803, 967
BsnI GGCC 5 cut(s) 28, 87, 124, 734, 779
Bso31I GGTCTC 1 cut(s) 735
BsoBI CYCGRG 1 cut(s) 493
Bsp1407I TGTACA 1 cut(s) 906
Bsp143I GATC 2 cut(s) 19, 609
BspACI CCGC 3 cut(s) 228, 231, 668
BspANI GGCC 5 cut(s) 28, 87, 124, 734, 779
BspCNI CTCAG 5 cut(s) 330, 370, 459, 676, 961
BspFNI CGCG 2 cut(s) 205, 637
BspLI GGNNCC 2 cut(s) 733, 968
BspPI GGATC 2 cut(s) 14, 604
BspTNI GGTCTC 1 cut(s) 735
BsrGI TGTACA 1 cut(s) 906
BsrI ACTGG 2 cut(s) 160, 1006
BssECI CCNNGG 3 cut(s) 23, 749, 962
BssHII GCGCGC 1 cut(s) 203
BssMI GATC 2 cut(s) 19, 609
BssT1I CCWWGG 2 cut(s) 23, 962
Bst4CI ACNGT 7 cut(s) 368, 386, 518, 599, 604, 675, 901
BstAUI TGTACA 1 cut(s) 906
BstC8I GCNNGC 3 cut(s) 205, 461, 924
BstDEI CTNAG 6 cut(s) 317, 378, 446, 663, 763, 948
BstDSI CCRYGG 1 cut(s) 749
BstF5I GGATG 3 cut(s) 223, 745, 958
BstFNI CGCG 2 cut(s) 205, 637
BstHHI GCGC 3 cut(s) 205, 207, 637
BstKTI GATC 2 cut(s) 22, 612
BstMAI GTCTC 4 cut(s) 358, 392, 596, 735
BstMBI GATC 2 cut(s) 19, 609
BstMWI GCNNNNNNNGC 2 cut(s) 335, 533
BstNSI RCATGY 1 cut(s) 926
BstSCI CCNGG 2 cut(s) 243, 551
BstUI CGCG 2 cut(s) 205, 637
BstV1I GCAGC 4 cut(s) 173, 277, 287, 382
BstXI CCANNNNNNTGG 2 cut(s) 59, 107
BsuI GTATCC 1 cut(s) 104
BsuRI GGCC 5 cut(s) 28, 87, 124, 734, 779
BtgI CCRYGG 1 cut(s) 749
BtsCI GGATG 3 cut(s) 223, 745, 958
BtsI GCAGTG 2 cut(s) 584, 719
BtsIMutI CAGTG 3 cut(s) 523, 584, 719
Cac8I GCNNGC 3 cut(s) 205, 461, 924
CfoI GCGC 3 cut(s) 205, 207, 637
Cfr13I GGNCC 2 cut(s) 732, 966
CseI GACGC 2 cut(s) 334, 578
Csp6I GTAC 3 cut(s) 600, 639, 907
CspCI CAANNNNNGTGG 2 cut(s) 452, 487
CviAII CATG 4 cut(s) 59, 263, 308, 923
CviQI GTAC 3 cut(s) 600, 639, 907
DdeI CTNAG 6 cut(s) 317, 378, 446, 663, 763, 948
DpnI GATC 2 cut(s) 21, 611
DpnII GATC 2 cut(s) 19, 609
DraI TTTAAA 1 cut(s) 579
EaeI YGGCCR 1 cut(s) 777
Eco130I CCWWGG 2 cut(s) 23, 962
Eco147I AGGCCT 3 cut(s) 28, 87, 124
Eco31I GGTCTC 1 cut(s) 735
Eco47I GGWCC 1 cut(s) 966
Eco88I CYCGRG 1 cut(s) 493
EcoO109I RGGNCCY 1 cut(s) 966
EcoT14I CCWWGG 2 cut(s) 23, 962
EcoT22I ATGCAT 1 cut(s) 928
ErhI CCWWGG 2 cut(s) 23, 962
Esp3I CGTCTC 2 cut(s) 358, 596
FaeI CATG 4 cut(s) 62, 266, 311, 926
FaqI GGGAC 3 cut(s) 128, 803, 967
FatI CATG 4 cut(s) 58, 262, 307, 922
FauI CCCGC 1 cut(s) 235
Fnu4HI GCNGC 6 cut(s) 162, 231, 291, 301, 396, 668
FokI GGATG 3 cut(s) 210, 732, 945
Fsp4HI GCNGC 6 cut(s) 162, 231, 291, 301, 396, 668
FspBI CTAG 5 cut(s) 138, 165, 876, 963, 977
GlaI GCGC 3 cut(s) 204, 206, 636
GluI GCNGC 6 cut(s) 162, 231, 291, 301, 396, 668
GsaI CCCAGC 2 cut(s) 222, 806
GsuI CTGGAG 1 cut(s) 177
HaeIII GGCC 5 cut(s) 28, 87, 124, 734, 779
HapII CCGG 2 cut(s) 245, 553
HgaI GACGC 2 cut(s) 334, 578
HhaI GCGC 3 cut(s) 205, 207, 637
Hin1II CATG 4 cut(s) 62, 266, 311, 926
Hin6I GCGC 3 cut(s) 203, 205, 635
HinP1I GCGC 3 cut(s) 203, 205, 635
HincII GTYRAC 1 cut(s) 415
HindII GTYRAC 1 cut(s) 415
HinfI GANTC 3 cut(s) 96, 273, 798
HpaII CCGG 2 cut(s) 245, 553
HphI GGTGA 5 cut(s) 181, 442, 524, 538, 641
Hpy166II GTNNAC 3 cut(s) 100, 256, 415
Hpy188I TCNGA 5 cut(s) 95, 453, 649, 700, 826
Hpy188III TCNNGA 1 cut(s) 493
Hpy8I GTNNAC 3 cut(s) 100, 256, 415
HpyAV CCTTC 3 cut(s) 25, 77, 135
HpyCH4III ACNGT 7 cut(s) 368, 386, 518, 599, 604, 675, 901
HpyCH4V TGCA 8 cut(s) 150, 486, 521, 659, 680, 756, 926, 940
HpyF10VI GCNNNNNNNGC 2 cut(s) 335, 533
HpyF3I CTNAG 6 cut(s) 317, 378, 446, 663, 763, 948
Hsp92II CATG 4 cut(s) 62, 266, 311, 926
HspAI GCGC 3 cut(s) 203, 205, 635
Kzo9I GATC 2 cut(s) 19, 609
LmnI GCTCC 3 cut(s) 158, 297, 856
LpnPI CCDG 8 cut(s) 141, 232, 258, 390, 405, 507, 566, 816
Lsp1109I GCAGC 4 cut(s) 173, 277, 287, 382
MaeI CTAG 5 cut(s) 138, 165, 876, 963, 977
MaeIII GTNAC 3 cut(s) 512, 544, 717
MalI GATC 2 cut(s) 21, 611
MboI GATC 2 cut(s) 19, 609
MboII GAAGA 2 cut(s) 26, 86
MlsI TGGCCA 1 cut(s) 779
MluCI AATT 6 cut(s) 78, 129, 185, 361, 850, 943
MluNI TGGCCA 1 cut(s) 779
MlyI GAGTC 1 cut(s) 105
MmeI TCCRAC 2 cut(s) 637, 810
MnlI CCTC 7 cut(s) 56, 251, 263, 326, 500, 620, 780
Mox20I TGGCCA 1 cut(s) 779
Mph1103I ATGCAT 1 cut(s) 928
MscI TGGCCA 1 cut(s) 779
MseI TTAA 3 cut(s) 360, 476, 578
MslI CAYNNNNRTG 3 cut(s) 57, 63, 105
Msp20I TGGCCA 1 cut(s) 779
MspI CCGG 2 cut(s) 245, 553
MspR9I CCNGG 2 cut(s) 245, 553
MvnI CGCG 2 cut(s) 205, 637
MwoI GCNNNNNNNGC 2 cut(s) 335, 533
NciI CCSGG 2 cut(s) 245, 553
NdeII GATC 2 cut(s) 19, 609
NlaIII CATG 4 cut(s) 62, 266, 311, 926
NlaIV GGNNCC 2 cut(s) 733, 968
NmuCI GTSAC 3 cut(s) 512, 544, 717
NsiI ATGCAT 1 cut(s) 928
NspI RCATGY 1 cut(s) 926
PaeI GCATGC 1 cut(s) 926
PaeR7I CTCGAG 1 cut(s) 493
PauI GCGCGC 1 cut(s) 203
PceI AGGCCT 3 cut(s) 28, 87, 124
PfeI GAWTC 2 cut(s) 273, 798
PflMI CCANNNNNTGG 1 cut(s) 198
PkrI GCNGC 6 cut(s) 163, 232, 292, 302, 397, 669
PleI GAGTC 1 cut(s) 104
PpsI GAGTC 1 cut(s) 104
PpuMI RGGWCCY 1 cut(s) 966
Psp5II RGGWCCY 1 cut(s) 966
PspFI CCCAGC 2 cut(s) 218, 802
PspN4I GGNNCC 2 cut(s) 733, 968
PspPI GGNCC 2 cut(s) 732, 966
PspPPI RGGWCCY 1 cut(s) 966
PteI GCGCGC 1 cut(s) 203
RsaI GTAC 3 cut(s) 601, 640, 908
RsaNI GTAC 3 cut(s) 600, 639, 907
RseI CAYNNNNRTG 3 cut(s) 57, 63, 105
SaqAI TTAA 3 cut(s) 360, 476, 578
SatI GCNGC 6 cut(s) 162, 231, 291, 301, 396, 668
Sau3AI GATC 2 cut(s) 19, 609
Sau96I GGNCC 2 cut(s) 732, 966
SchI GAGTC 1 cut(s) 105
ScrFI CCNGG 2 cut(s) 245, 553
Sfr274I CTCGAG 1 cut(s) 493
SinI GGWCC 1 cut(s) 966
SlaI CTCGAG 1 cut(s) 493
SmiMI CAYNNNNRTG 3 cut(s) 57, 63, 105
SmlI CTYRAG 2 cut(s) 493, 793
SmoI CTYRAG 2 cut(s) 493, 793
SpeI ACTAGT 1 cut(s) 976
SphI GCATGC 1 cut(s) 926
Sse9I AATT 6 cut(s) 78, 129, 185, 361, 850, 943
SseBI AGGCCT 3 cut(s) 28, 87, 124
SsiI CCGC 3 cut(s) 228, 231, 668
SspMI CTAG 5 cut(s) 138, 165, 876, 963, 977
StuI AGGCCT 3 cut(s) 28, 87, 124
StyD4I CCNGG 2 cut(s) 243, 551
StyI CCWWGG 2 cut(s) 23, 962
TaaI ACNGT 7 cut(s) 368, 386, 518, 599, 604, 675, 901
TaqI TCGA 2 cut(s) 18, 494
TasI AATT 6 cut(s) 78, 129, 185, 361, 850, 943
TatI WGTACW 1 cut(s) 906
TauI GCSGC 2 cut(s) 233, 670
TfiI GAWTC 2 cut(s) 273, 798
Tru1I TTAA 3 cut(s) 360, 476, 578
Tru9I TTAA 3 cut(s) 360, 476, 578
TscAI CASTG 3 cut(s) 523, 591, 726
TseFI GTSAC 3 cut(s) 512, 544, 717
TseI GCWGC 4 cut(s) 161, 290, 300, 395
Tsp45I GTSAC 3 cut(s) 512, 544, 717
TspDTI ATGAA 4 cut(s) 251, 493, 579, 988
TspGWI ACGGA 1 cut(s) 738
TspRI CASTG 3 cut(s) 523, 591, 726
Van91I CCANNNNNTGG 1 cut(s) 198
VpaK11BI GGWCC 1 cut(s) 966
XapI RAATTY 2 cut(s) 185, 850
XceI RCATGY 1 cut(s) 926
XcmI CCANNNNNNNNNTGG 1 cut(s) 620
XhoI CTCGAG 1 cut(s) 493
XmaJI CCTAGG 1 cut(s) 962
XspI CTAG 5 cut(s) 138, 165, 876, 963, 977
Zsp2I ATGCAT 1 cut(s) 928
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.