RchiOBHm_Chr2g0162961

carboxylesterase 17

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0009064
Physical Location & Seq
Reverse (-)
78652966 .. 78653994
1029 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ53117

Sequence Viewer

Length: 1029 bp
ATGCGATTTAGAAGATCAATGGCAACCATTTCCTTCGATCCAAGGCCAAATCTTCAAGCAACTAACAACCATCAACATGGAGGTGTTGTTGAAGAAATTGAAGGCCTTATCAGAGTCCACAAAAATGGATACATAGAAAGGCCTTCAATTATCCCTAGTGTCCCTTGCACTACTGCAGCAGCTAGAGATGTTGTCATTGACAACTTCACCAACTTGTGGGGGCGTATTTATGTTCCCAGCCATCCCGCGGCCAACAACCTCTCGGTGCTTGTTTACTTTCACGGTGGAGGATTCTGTGTTGGCTCTGCTGCTTGGAGCTGCTACCATGAGTTCCTCAGCAACCTTGCTTGTAAAGCAAGTTGTGTCATCGTCTCTGTTAATTACCGTTTAGCCCCGGAGAACCGTCTCCCTGCTGCCTATGATGATGGTGTCAACGCTGTTATGTGGGTGAAACAGCAAGCTCTCAGTCAGAGTTGTGAGCAAAAATGGTGGTTAAGTCGGTGCAACCTCTCGAGCTTGTTCATAGGTGGTGACAGTGCAGGGGCTAACATAGCCTACAATGTCACCACCCAGTTGTCAAATGAACAGTCGGTTTTAAAACCACTGCGTCTCAAAGGTACTGTTTTGATCCAACCTTTCTTTGGAGGTGAAGCGCGTACTTGGTCTGAAAAATATGCAACTCAGCCGTCACAGTCTGCACTAACTCTATCAAACTCCGATGTGTATTGGCGATTGTCACTGCCGTTGGGGGCCAACCGAGACCATCCGTGGTGCAATCCCTTAGCGAATGGTGTGGCCAAGTTGAGGGACTTGAGAATCCCAGCTATAATGGTGTGCGTATCAGAGTTGGATATATTGAAGGATAGGAATTTGGAGCTGTGTAATGCTTTGACTAGCTTGGGAAAAAAGGTGGAAACAGTAATGTACAAAGGGGTTGGGCATGCATTTCAAGTTCTGCATAATTCTCAGCTCTCTCATCCTAGGACCCAAGAACTAGTGTCCCACATCAAGGCTTTCATAAACCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

342

Amino Acids

37.92

Weight (kDa)

8.89

Isoelectric Point (pI)

43.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BD-FAE PF20434 76 - 190 3.2e-11 BD-FAE
COesterase PF00135 76 - 132 3.7e-09 Carboxylesterase family
Abhydrolase_3 PF07859 90 - 317 5.5e-60 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 216
AccII CGCG 2 cut(s) 248, 655
AciI CCGC 2 cut(s) 246, 248
AclWI GGATC 2 cut(s) 32, 622
AcoI YGGCCR 2 cut(s) 249, 795
AcsI RAATTY 1 cut(s) 868
AfaI GTAC 3 cut(s) 619, 658, 926
AfiI CCNNNNNNNGG 5 cut(s) 216, 247, 641, 804, 1009
AgsI TTSAA 6 cut(s) 56, 92, 101, 147, 859, 950
AhlI ACTAGT 1 cut(s) 994
AluBI AGCT 8 cut(s) 182, 318, 461, 516, 824, 877, 897, 970
AluI AGCT 8 cut(s) 182, 318, 461, 516, 824, 877, 897, 970
Alw26I GTCTC 4 cut(s) 376, 410, 614, 753
AlwI GGATC 2 cut(s) 32, 622
Ama87I CYCGRG 1 cut(s) 511
AoxI GGCC 6 cut(s) 44, 103, 140, 249, 750, 795
ApeKI GCWGC 5 cut(s) 176, 179, 308, 318, 413
ApoI RAATTY 1 cut(s) 868
ArsI GACNNNNNNTTYG 2 cut(s) 572, 604
AspA2I CCTAGG 1 cut(s) 980
AspLEI GCGC 1 cut(s) 655
AspS9I GGNCC 2 cut(s) 750, 984
AsuC2I CCSGG 1 cut(s) 395
AsuHPI GGTGA 5 cut(s) 199, 460, 542, 556, 659
AvaI CYCGRG 1 cut(s) 511
AvaII GGWCC 1 cut(s) 984
AvrII CCTAGG 1 cut(s) 980
BalI TGGCCA 1 cut(s) 797
BbvCI CCTCAGC 1 cut(s) 335
BbvI GCAGC 5 cut(s) 188, 191, 295, 305, 400
BccI CCATC 4 cut(s) 78, 249, 419, 771
BceAI ACGGC 2 cut(s) 670, 727
BciVI GTATCC 1 cut(s) 122
BcnI CCSGG 1 cut(s) 395
BcoDI GTCTC 4 cut(s) 376, 410, 614, 753
BcuI ACTAGT 1 cut(s) 994
BfaI CTAG 5 cut(s) 156, 183, 894, 981, 995
BfmI CTRYAG 1 cut(s) 174
BfuI GTATCC 1 cut(s) 122
BisI GCNGC 6 cut(s) 177, 180, 249, 309, 319, 414
BlnI CCTAGG 1 cut(s) 980
BlsI GCNGC 6 cut(s) 178, 181, 250, 310, 320, 415
Bme1390I CCNGG 1 cut(s) 395
Bme18I GGWCC 1 cut(s) 984
BmeT110I CYCGRG 1 cut(s) 511
BmgT120I GGNCC 2 cut(s) 750, 984
BmiI GGNNCC 2 cut(s) 751, 986
BmrFI CCNGG 1 cut(s) 395
BmrI ACTGGG 1 cut(s) 565
BmuI ACTGGG 1 cut(s) 565
Bpu10I CCTNAGC 2 cut(s) 335, 781
BpuEI CTTGAG 1 cut(s) 832
BpuMI CCSGG 1 cut(s) 395
BsaI GGTCTC 1 cut(s) 753
BsaJI CCNNGG 5 cut(s) 41, 246, 393, 767, 980
BsaXI ACNNNNNCTCC 2 cut(s) 72, 102
Bsc4I CCNNNNNNNGG 5 cut(s) 216, 247, 641, 804, 1009
Bse1I ACTGG 2 cut(s) 571, 1024
BseDI CCNNGG 5 cut(s) 41, 246, 393, 767, 980
BseGI GGATG 3 cut(s) 241, 763, 976
BseLI CCNNNNNNNGG 5 cut(s) 216, 247, 641, 804, 1009
BseMII CTCAG 4 cut(s) 349, 478, 695, 980
BseNI ACTGG 2 cut(s) 571, 1024
BseXI GCAGC 5 cut(s) 188, 191, 295, 305, 400
BseYI CCCAGC 2 cut(s) 236, 820
BsgI GTGCAG 2 cut(s) 558, 681
Bsh1236I CGCG 2 cut(s) 248, 655
BshFI GGCC 6 cut(s) 46, 105, 142, 251, 752, 797
BsiHKCI CYCGRG 1 cut(s) 511
BsiSI CCGG 1 cut(s) 395
BslFI GGGAC 3 cut(s) 146, 821, 985
BslI CCNNNNNNNGG 5 cut(s) 216, 247, 641, 804, 1009
BsmAI GTCTC 4 cut(s) 376, 410, 614, 753
BsmBI CGTCTC 3 cut(s) 376, 410, 614
BsmFI GGGAC 3 cut(s) 146, 821, 985
BsnI GGCC 6 cut(s) 46, 105, 142, 251, 752, 797
Bso31I GGTCTC 1 cut(s) 753
BsoBI CYCGRG 1 cut(s) 511
Bsp1407I TGTACA 1 cut(s) 924
Bsp143I GATC 3 cut(s) 14, 37, 627
BspACI CCGC 2 cut(s) 246, 248
BspANI GGCC 6 cut(s) 46, 105, 142, 251, 752, 797
BspCNI CTCAG 4 cut(s) 348, 477, 694, 979
BspFNI CGCG 2 cut(s) 248, 655
BspLI GGNNCC 2 cut(s) 751, 986
BspMAI CTGCAG 1 cut(s) 178
BspPI GGATC 2 cut(s) 32, 622
BspTNI GGTCTC 1 cut(s) 753
BsrGI TGTACA 1 cut(s) 924
BsrI ACTGG 2 cut(s) 571, 1024
BssECI CCNNGG 5 cut(s) 41, 246, 393, 767, 980
BssMI GATC 3 cut(s) 14, 37, 627
BssT1I CCWWGG 2 cut(s) 41, 980
Bst4CI ACNGT 8 cut(s) 284, 386, 404, 536, 588, 622, 693, 919
BstAUI TGTACA 1 cut(s) 924
BstC8I GCNNGC 2 cut(s) 459, 942
BstDEI CTNAG 5 cut(s) 335, 464, 681, 781, 966
BstDSI CCRYGG 2 cut(s) 246, 767
BstF5I GGATG 3 cut(s) 241, 763, 976
BstFNI CGCG 2 cut(s) 248, 655
BstHHI GCGC 1 cut(s) 655
BstKTI GATC 3 cut(s) 17, 40, 630
BstMAI GTCTC 4 cut(s) 376, 410, 614, 753
BstMBI GATC 3 cut(s) 14, 37, 627
BstMWI GCNNNNNNNGC 2 cut(s) 353, 551
BstNSI RCATGY 1 cut(s) 944
BstSCI CCNGG 1 cut(s) 393
BstSFI CTRYAG 1 cut(s) 174
BstUI CGCG 2 cut(s) 248, 655
BstV1I GCAGC 5 cut(s) 188, 191, 295, 305, 400
BstXI CCANNNNNNTGG 2 cut(s) 77, 125
BsuI GTATCC 1 cut(s) 122
BsuRI GGCC 6 cut(s) 46, 105, 142, 251, 752, 797
BtgI CCRYGG 2 cut(s) 246, 767
BtsCI GGATG 3 cut(s) 241, 763, 976
BtsI GCAGTG 2 cut(s) 602, 737
BtsIMutI CAGTG 3 cut(s) 541, 602, 737
Cac8I GCNNGC 2 cut(s) 459, 942
CfoI GCGC 1 cut(s) 655
Cfr13I GGNCC 2 cut(s) 750, 984
Cfr42I CCGCGG 1 cut(s) 249
CseI GACGC 1 cut(s) 596
Csp6I GTAC 3 cut(s) 618, 657, 925
CspCI CAANNNNNGTGG 2 cut(s) 470, 505
CviAII CATG 3 cut(s) 77, 326, 941
CviQI GTAC 3 cut(s) 618, 657, 925
DdeI CTNAG 5 cut(s) 335, 464, 681, 781, 966
DpnI GATC 3 cut(s) 16, 39, 629
DpnII GATC 3 cut(s) 14, 37, 627
DraI TTTAAA 1 cut(s) 597
EaeI YGGCCR 2 cut(s) 249, 795
Eco130I CCWWGG 2 cut(s) 41, 980
Eco147I AGGCCT 2 cut(s) 105, 142
Eco31I GGTCTC 1 cut(s) 753
Eco47I GGWCC 1 cut(s) 984
Eco88I CYCGRG 1 cut(s) 511
EcoO109I RGGNCCY 1 cut(s) 984
EcoT14I CCWWGG 2 cut(s) 41, 980
EcoT22I ATGCAT 1 cut(s) 946
ErhI CCWWGG 2 cut(s) 41, 980
Esp3I CGTCTC 3 cut(s) 376, 410, 614
FaeI CATG 3 cut(s) 80, 329, 944
FaqI GGGAC 3 cut(s) 146, 821, 985
FatI CATG 3 cut(s) 76, 325, 940
FauI CCCGC 1 cut(s) 253
Fnu4HI GCNGC 6 cut(s) 177, 180, 249, 309, 319, 414
FokI GGATG 3 cut(s) 228, 750, 963
Fsp4HI GCNGC 6 cut(s) 177, 180, 249, 309, 319, 414
FspBI CTAG 5 cut(s) 156, 183, 894, 981, 995
GlaI GCGC 1 cut(s) 654
GluI GCNGC 6 cut(s) 177, 180, 249, 309, 319, 414
GsaI CCCAGC 2 cut(s) 240, 824
HaeIII GGCC 6 cut(s) 46, 105, 142, 251, 752, 797
HapII CCGG 1 cut(s) 395
HgaI GACGC 1 cut(s) 596
HhaI GCGC 1 cut(s) 655
Hin1II CATG 3 cut(s) 80, 329, 944
Hin6I GCGC 1 cut(s) 653
HinP1I GCGC 1 cut(s) 653
HincII GTYRAC 1 cut(s) 433
HindII GTYRAC 1 cut(s) 433
HinfI GANTC 3 cut(s) 114, 291, 816
HpaII CCGG 1 cut(s) 395
HphI GGTGA 5 cut(s) 199, 460, 542, 556, 659
Hpy166II GTNNAC 3 cut(s) 118, 274, 433
Hpy188I TCNGA 5 cut(s) 113, 471, 667, 718, 844
Hpy188III TCNNGA 1 cut(s) 511
Hpy8I GTNNAC 3 cut(s) 118, 274, 433
HpyAV CCTTC 4 cut(s) 43, 95, 153, 853
HpyCH4III ACNGT 8 cut(s) 284, 386, 404, 536, 588, 622, 693, 919
HpyCH4V TGCA 9 cut(s) 168, 176, 504, 539, 677, 698, 774, 944, 958
HpyF10VI GCNNNNNNNGC 2 cut(s) 353, 551
HpyF3I CTNAG 5 cut(s) 335, 464, 681, 781, 966
Hsp92II CATG 3 cut(s) 80, 329, 944
HspAI GCGC 1 cut(s) 653
KspI CCGCGG 1 cut(s) 249
Kzo9I GATC 3 cut(s) 14, 37, 627
LmnI GCTCC 2 cut(s) 315, 874
LpnPI CCDG 6 cut(s) 250, 408, 423, 525, 584, 834
Lsp1109I GCAGC 5 cut(s) 188, 191, 295, 305, 400
MaeI CTAG 5 cut(s) 156, 183, 894, 981, 995
MaeIII GTNAC 4 cut(s) 530, 562, 687, 735
MalI GATC 3 cut(s) 16, 39, 629
MboI GATC 3 cut(s) 14, 37, 627
MboII GAAGA 3 cut(s) 24, 44, 104
MlsI TGGCCA 1 cut(s) 797
MluCI AATT 5 cut(s) 96, 147, 379, 868, 961
MluNI TGGCCA 1 cut(s) 797
MlyI GAGTC 1 cut(s) 123
MmeI TCCRAC 2 cut(s) 655, 828
MnlI CCTC 7 cut(s) 74, 269, 281, 344, 518, 638, 798
Mox20I TGGCCA 1 cut(s) 797
Mph1103I ATGCAT 1 cut(s) 946
MscI TGGCCA 1 cut(s) 797
MseI TTAA 3 cut(s) 378, 494, 596
MslI CAYNNNNRTG 3 cut(s) 75, 81, 123
Msp20I TGGCCA 1 cut(s) 797
MspA1I CMGCKG 1 cut(s) 248
MspI CCGG 1 cut(s) 395
MspR9I CCNGG 1 cut(s) 395
MvnI CGCG 2 cut(s) 248, 655
MwoI GCNNNNNNNGC 2 cut(s) 353, 551
NciI CCSGG 1 cut(s) 395
NdeII GATC 3 cut(s) 14, 37, 627
NlaIII CATG 3 cut(s) 80, 329, 944
NlaIV GGNNCC 2 cut(s) 751, 986
NmuCI GTSAC 4 cut(s) 530, 562, 687, 735
NsiI ATGCAT 1 cut(s) 946
NspI RCATGY 1 cut(s) 944
PaeI GCATGC 1 cut(s) 944
PaeR7I CTCGAG 1 cut(s) 511
PceI AGGCCT 2 cut(s) 105, 142
PfeI GAWTC 2 cut(s) 291, 816
PflMI CCANNNNNTGG 1 cut(s) 216
PkrI GCNGC 6 cut(s) 178, 181, 250, 310, 320, 415
PleI GAGTC 1 cut(s) 122
PpsI GAGTC 1 cut(s) 122
PpuMI RGGWCCY 1 cut(s) 984
Psp5II RGGWCCY 1 cut(s) 984
PspFI CCCAGC 2 cut(s) 236, 820
PspN4I GGNNCC 2 cut(s) 751, 986
PspPI GGNCC 2 cut(s) 750, 984
PspPPI RGGWCCY 1 cut(s) 984
PstI CTGCAG 1 cut(s) 178
RsaI GTAC 3 cut(s) 619, 658, 926
RsaNI GTAC 3 cut(s) 618, 657, 925
RseI CAYNNNNRTG 3 cut(s) 75, 81, 123
SacII CCGCGG 1 cut(s) 249
SaqAI TTAA 3 cut(s) 378, 494, 596
SatI GCNGC 6 cut(s) 177, 180, 249, 309, 319, 414
Sau3AI GATC 3 cut(s) 14, 37, 627
Sau96I GGNCC 2 cut(s) 750, 984
SchI GAGTC 1 cut(s) 123
ScrFI CCNGG 1 cut(s) 395
SfcI CTRYAG 1 cut(s) 174
Sfr274I CTCGAG 1 cut(s) 511
Sfr303I CCGCGG 1 cut(s) 249
SgrBI CCGCGG 1 cut(s) 249
SinI GGWCC 1 cut(s) 984
SlaI CTCGAG 1 cut(s) 511
SmiMI CAYNNNNRTG 3 cut(s) 75, 81, 123
SmlI CTYRAG 2 cut(s) 511, 811
SmoI CTYRAG 2 cut(s) 511, 811
SpeI ACTAGT 1 cut(s) 994
SphI GCATGC 1 cut(s) 944
Sse9I AATT 5 cut(s) 96, 147, 379, 868, 961
SseBI AGGCCT 2 cut(s) 105, 142
SsiI CCGC 2 cut(s) 246, 248
SspMI CTAG 5 cut(s) 156, 183, 894, 981, 995
StuI AGGCCT 2 cut(s) 105, 142
StyD4I CCNGG 1 cut(s) 393
StyI CCWWGG 2 cut(s) 41, 980
TaaI ACNGT 8 cut(s) 284, 386, 404, 536, 588, 622, 693, 919
TaqI TCGA 2 cut(s) 36, 512
TasI AATT 5 cut(s) 96, 147, 379, 868, 961
TatI WGTACW 1 cut(s) 924
TauI GCSGC 1 cut(s) 251
TfiI GAWTC 2 cut(s) 291, 816
Tru1I TTAA 3 cut(s) 378, 494, 596
Tru9I TTAA 3 cut(s) 378, 494, 596
TscAI CASTG 3 cut(s) 541, 609, 744
TseFI GTSAC 4 cut(s) 530, 562, 687, 735
TseI GCWGC 5 cut(s) 176, 179, 308, 318, 413
Tsp45I GTSAC 4 cut(s) 530, 562, 687, 735
TspDTI ATGAA 3 cut(s) 511, 597, 1006
TspGWI ACGGA 1 cut(s) 756
TspRI CASTG 3 cut(s) 541, 609, 744
Van91I CCANNNNNTGG 1 cut(s) 216
VpaK11BI GGWCC 1 cut(s) 984
XapI RAATTY 1 cut(s) 868
XceI RCATGY 1 cut(s) 944
XcmI CCANNNNNNNNNTGG 1 cut(s) 638
XhoI CTCGAG 1 cut(s) 511
XmaJI CCTAGG 1 cut(s) 980
XspI CTAG 5 cut(s) 156, 183, 894, 981, 995
Zsp2I ATGCAT 1 cut(s) 946
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.