RchiOBHm_Chr2g0162951

carboxylesterase 17

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0009064
Physical Location & Seq
Reverse (-)
78623924 .. 78624963
1040 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ53116

Sequence Viewer

Length: 1035 bp
ATGCGATTTAGAAGATCAATGGCAACCATTTCCTTCAATCCGAGGCCTAATCTTCAAGCAACTAACAACCATAGCCATCAACATGGAGTTGTTGTTGAAGAAATTGAAGGCTTTATCAGAGTCCACAAAAATGGATACATAGAAAGGCATTCAATTATCCCTAGTGTCCCTTGCACTACTGCAACAGCTAGAGATGTTGTCATTGACAAATTCTCCAACTTGTGGGCGCGTATTTATGTTCCCAGCCACCCAGGGGCCAATAATCTCCCGGTGCTTCTTTACTTTCATGGTGGAGGATTCTGTGTTGGTTCTGCTGCTTGGAGCTGCTACCATGAGTTCCTCAGCAACCTTGCTTGTAAAGCAAGTTGTGTCATCGTCTCTGTAAATTACCGTTTAGCCCCTGAGAACCGTCTCCCTGCTGCCTATGATGATGGTGTCAACGCTGTTATGTGGGTGAAACAACAAGCTCTCAGTCAGAGTTGCGAGCAAAAATGGTGGTTAAGTCGGTGCAACCTCTCGAGCTTTTTCATAAGTGGTGACAGTGCAGGGGCTAACATAGCCTACAATGTCACCACCCGGTTATCAAATGAACACTCGGTTTTAAAACCATTGCGTCTCAAAGGTACTGTTCTGATCCAACCTTTCTTTGGAGGTGAAGCACGTACTTGGTCTGAAAAACATGCAACTCAGCCACCACACTCTGCACTAACTCTATCAAACTCCGATGTGTATTGGCGACTGTCACTGCCGTTGGGGGCCAACCGAGACCATCCGTGGTGCAATCCCTTAGCAAATGGCTTGGCAAAGTTGAGGGATTTGAGACTCCCAGCTATAATGGTGTGCGTATCAGAGTTGGATATATTGAAAGACAGGAATTTGGAGCTGTGCAATGTTTTGACTAGATTGGGGAAAATGGTCGAAACAGTAACGTACGAGGGGGTTGGGCATGCATTTCAAGTTCTGCATAACTCTCAGCTCTCTCATCCTAGGACCCAAGAATTGGTGTCTCACATCAAGGTTTTCATAAACCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

344

Amino Acids

38.52

Weight (kDa)

9.01

Isoelectric Point (pI)

39.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BD-FAE PF20434 78 - 191 7.6e-12 BD-FAE
COesterase PF00135 78 - 134 5.5e-09 Carboxylesterase family
Abhydrolase_3 PF07859 92 - 319 1.7e-58 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 222, 1000
AccII CGCG 1 cut(s) 229
AclWI GGATC 1 cut(s) 628
AcsI RAATTY 2 cut(s) 209, 874
AfaI GTAC 3 cut(s) 625, 664, 932
AfiI CCNNNNNNNGG 4 cut(s) 222, 253, 647, 1000
AgsI TTSAA 7 cut(s) 37, 56, 98, 107, 153, 865, 956
AjnI CCWGG 1 cut(s) 250
AluBI AGCT 7 cut(s) 188, 324, 467, 522, 830, 883, 976
AluI AGCT 7 cut(s) 188, 324, 467, 522, 830, 883, 976
Alw26I GTCTC 6 cut(s) 382, 416, 620, 759, 814, 1011
AlwI GGATC 1 cut(s) 628
Ama87I CYCGRG 1 cut(s) 517
AoxI GGCC 3 cut(s) 44, 255, 756
ApeKI GCWGC 3 cut(s) 314, 324, 419
ApoI RAATTY 2 cut(s) 209, 874
AspA2I CCTAGG 1 cut(s) 986
AspLEI GCGC 1 cut(s) 229
AspS9I GGNCC 3 cut(s) 255, 756, 990
AsuC2I CCSGG 2 cut(s) 269, 577
AsuHPI GGTGA 4 cut(s) 466, 548, 562, 665
AvaI CYCGRG 1 cut(s) 517
AvaII GGWCC 1 cut(s) 990
AvrII CCTAGG 1 cut(s) 986
BbvCI CCTCAGC 1 cut(s) 341
BbvI GCAGC 3 cut(s) 301, 311, 406
BccI CCATC 3 cut(s) 84, 425, 777
BceAI ACGGC 1 cut(s) 733
BciT130I CCWGG 1 cut(s) 252
BciVI GTATCC 1 cut(s) 128
BcnI CCSGG 2 cut(s) 269, 577
BcoDI GTCTC 6 cut(s) 382, 416, 620, 759, 814, 1011
BfaI CTAG 4 cut(s) 162, 189, 900, 987
BfuI GTATCC 1 cut(s) 128
BisI GCNGC 3 cut(s) 315, 325, 420
BlnI CCTAGG 1 cut(s) 986
BlsI GCNGC 3 cut(s) 316, 326, 421
Bme1390I CCNGG 3 cut(s) 252, 269, 577
Bme18I GGWCC 1 cut(s) 990
BmeT110I CYCGRG 1 cut(s) 517
BmgT120I GGNCC 3 cut(s) 255, 756, 990
BmiI GGNNCC 3 cut(s) 256, 757, 992
BmrFI CCNGG 3 cut(s) 252, 269, 577
Bpu10I CCTNAGC 2 cut(s) 341, 787
BpuMI CCSGG 2 cut(s) 269, 577
BsaAI YACGTR 1 cut(s) 662
BsaI GGTCTC 1 cut(s) 759
BsaJI CCNNGG 5 cut(s) 41, 250, 251, 773, 986
BsaXI ACNNNNNCTCC 4 cut(s) 78, 108, 197, 227
Bsc4I CCNNNNNNNGG 4 cut(s) 222, 253, 647, 1000
Bse1I ACTGG 1 cut(s) 1030
Bse3DI GCAATG 2 cut(s) 608, 895
BseBI CCWGG 1 cut(s) 252
BseDI CCNNGG 5 cut(s) 41, 250, 251, 773, 986
BseGI GGATG 2 cut(s) 769, 982
BseLI CCNNNNNNNGG 4 cut(s) 222, 253, 647, 1000
BseMI GCAATG 2 cut(s) 608, 895
BseMII CTCAG 5 cut(s) 355, 393, 484, 701, 986
BseNI ACTGG 1 cut(s) 1030
BseXI GCAGC 3 cut(s) 301, 311, 406
BseYI CCCAGC 2 cut(s) 242, 826
BsgI GTGCAG 2 cut(s) 564, 687
Bsh1236I CGCG 1 cut(s) 229
BshFI GGCC 3 cut(s) 46, 257, 758
BsiHKCI CYCGRG 1 cut(s) 517
BsiSI CCGG 2 cut(s) 269, 577
BsiWI CGTACG 1 cut(s) 930
BslFI GGGAC 1 cut(s) 152
BslI CCNNNNNNNGG 4 cut(s) 222, 253, 647, 1000
BsmAI GTCTC 6 cut(s) 382, 416, 620, 759, 814, 1011
BsmBI CGTCTC 3 cut(s) 382, 416, 620
BsmFI GGGAC 1 cut(s) 152
BsmI GAATGC 1 cut(s) 148
BsnI GGCC 3 cut(s) 46, 257, 758
Bso31I GGTCTC 1 cut(s) 759
BsoBI CYCGRG 1 cut(s) 517
Bsp143I GATC 2 cut(s) 14, 633
BspANI GGCC 3 cut(s) 46, 257, 758
BspCNI CTCAG 5 cut(s) 354, 394, 483, 700, 985
BspFNI CGCG 1 cut(s) 229
BspLI GGNNCC 3 cut(s) 256, 757, 992
BspPI GGATC 1 cut(s) 628
BspTNI GGTCTC 1 cut(s) 759
BsrDI GCAATG 2 cut(s) 608, 895
BsrI ACTGG 1 cut(s) 1030
BssECI CCNNGG 5 cut(s) 41, 250, 251, 773, 986
BssMI GATC 2 cut(s) 14, 633
BssT1I CCWWGG 1 cut(s) 986
Bst2UI CCWGG 1 cut(s) 252
Bst4CI ACNGT 6 cut(s) 392, 410, 542, 628, 741, 925
BstBAI YACGTR 1 cut(s) 662
BstC8I GCNNGC 2 cut(s) 485, 948
BstDEI CTNAG 6 cut(s) 341, 402, 470, 687, 787, 972
BstDSI CCRYGG 1 cut(s) 773
BstF5I GGATG 2 cut(s) 769, 982
BstFNI CGCG 1 cut(s) 229
BstHHI GCGC 1 cut(s) 229
BstKTI GATC 2 cut(s) 17, 636
BstMAI GTCTC 6 cut(s) 382, 416, 620, 759, 814, 1011
BstMBI GATC 2 cut(s) 14, 633
BstMWI GCNNNNNNNGC 2 cut(s) 359, 557
BstNI CCWGG 1 cut(s) 252
BstNSI RCATGY 2 cut(s) 683, 950
BstSCI CCNGG 3 cut(s) 250, 267, 575
BstUI CGCG 1 cut(s) 229
BstV1I GCAGC 3 cut(s) 301, 311, 406
BstXI CCANNNNNNTGG 2 cut(s) 83, 131
BsuI GTATCC 1 cut(s) 128
BsuRI GGCC 3 cut(s) 46, 257, 758
BtgI CCRYGG 1 cut(s) 773
BtsCI GGATG 2 cut(s) 769, 982
BtsI GCAGTG 1 cut(s) 743
BtsIMutI CAGTG 2 cut(s) 547, 743
Cac8I GCNNGC 2 cut(s) 485, 948
CfoI GCGC 1 cut(s) 229
Cfr13I GGNCC 3 cut(s) 255, 756, 990
CseI GACGC 1 cut(s) 602
Csp6I GTAC 3 cut(s) 624, 663, 931
CspCI CAANNNNNGTGG 2 cut(s) 476, 511
CviAII CATG 5 cut(s) 83, 287, 332, 680, 947
CviQI GTAC 3 cut(s) 624, 663, 931
DdeI CTNAG 6 cut(s) 341, 402, 470, 687, 787, 972
DpnI GATC 2 cut(s) 16, 635
DpnII GATC 2 cut(s) 14, 633
DraI TTTAAA 1 cut(s) 603
Eco130I CCWWGG 1 cut(s) 986
Eco147I AGGCCT 1 cut(s) 46
Eco31I GGTCTC 1 cut(s) 759
Eco47I GGWCC 1 cut(s) 990
Eco88I CYCGRG 1 cut(s) 517
EcoO109I RGGNCCY 1 cut(s) 990
EcoRII CCWGG 1 cut(s) 250
EcoT14I CCWWGG 1 cut(s) 986
EcoT22I ATGCAT 1 cut(s) 952
ErhI CCWWGG 1 cut(s) 986
Esp3I CGTCTC 3 cut(s) 382, 416, 620
FaeI CATG 5 cut(s) 86, 290, 335, 683, 950
FaqI GGGAC 1 cut(s) 152
FatI CATG 5 cut(s) 82, 286, 331, 679, 946
Fnu4HI GCNGC 3 cut(s) 315, 325, 420
FokI GGATG 2 cut(s) 756, 969
Fsp4HI GCNGC 3 cut(s) 315, 325, 420
FspBI CTAG 4 cut(s) 162, 189, 900, 987
GlaI GCGC 1 cut(s) 228
GluI GCNGC 3 cut(s) 315, 325, 420
GsaI CCCAGC 2 cut(s) 246, 830
HaeIII GGCC 3 cut(s) 46, 257, 758
HapII CCGG 2 cut(s) 269, 577
HgaI GACGC 1 cut(s) 602
HhaI GCGC 1 cut(s) 229
Hin1II CATG 5 cut(s) 86, 290, 335, 683, 950
Hin6I GCGC 1 cut(s) 227
HinP1I GCGC 1 cut(s) 227
HincII GTYRAC 1 cut(s) 439
HindII GTYRAC 1 cut(s) 439
HinfI GANTC 3 cut(s) 120, 297, 822
HpaII CCGG 2 cut(s) 269, 577
HphI GGTGA 4 cut(s) 466, 548, 562, 665
Hpy166II GTNNAC 2 cut(s) 124, 439
Hpy188I TCNGA 7 cut(s) 42, 119, 477, 633, 673, 724, 850
Hpy188III TCNNGA 1 cut(s) 517
Hpy8I GTNNAC 2 cut(s) 124, 439
HpyAV CCTTC 2 cut(s) 43, 101
HpyCH4III ACNGT 6 cut(s) 392, 410, 542, 628, 741, 925
HpyCH4IV ACGT 2 cut(s) 661, 929
HpyF10VI GCNNNNNNNGC 2 cut(s) 359, 557
HpyF3I CTNAG 6 cut(s) 341, 402, 470, 687, 787, 972
HpySE526I ACGT 2 cut(s) 661, 929
Hsp92II CATG 5 cut(s) 86, 290, 335, 683, 950
HspAI GCGC 1 cut(s) 227
Kzo9I GATC 2 cut(s) 14, 633
LmnI GCTCC 2 cut(s) 321, 880
Lsp1109I GCAGC 3 cut(s) 301, 311, 406
MaeI CTAG 4 cut(s) 162, 189, 900, 987
MaeII ACGT 2 cut(s) 661, 929
MaeIII GTNAC 4 cut(s) 536, 568, 741, 925
MalI GATC 2 cut(s) 16, 635
MboI GATC 2 cut(s) 14, 633
MboII GAAGA 3 cut(s) 24, 44, 110
MluCI AATT 6 cut(s) 102, 153, 209, 385, 874, 998
MlyI GAGTC 2 cut(s) 129, 816
MmeI TCCRAC 3 cut(s) 240, 661, 834
MnlI CCTC 7 cut(s) 36, 287, 350, 524, 644, 804, 928
Mph1103I ATGCAT 1 cut(s) 952
MseI TTAA 2 cut(s) 500, 602
MslI CAYNNNNRTG 2 cut(s) 81, 129
MspI CCGG 2 cut(s) 269, 577
MspR9I CCNGG 3 cut(s) 252, 269, 577
Mva1269I GAATGC 1 cut(s) 148
MvaI CCWGG 1 cut(s) 252
MvnI CGCG 1 cut(s) 229
MwoI GCNNNNNNNGC 2 cut(s) 359, 557
NciI CCSGG 2 cut(s) 269, 577
NdeII GATC 2 cut(s) 14, 633
NlaIII CATG 5 cut(s) 86, 290, 335, 683, 950
NlaIV GGNNCC 3 cut(s) 256, 757, 992
NmuCI GTSAC 3 cut(s) 536, 568, 741
NsiI ATGCAT 1 cut(s) 952
NspI RCATGY 2 cut(s) 683, 950
PaeI GCATGC 1 cut(s) 950
PaeR7I CTCGAG 1 cut(s) 517
PasI CCCWGGG 1 cut(s) 251
PceI AGGCCT 1 cut(s) 46
PctI GAATGC 1 cut(s) 148
PfeI GAWTC 1 cut(s) 297
Pfl23II CGTACG 1 cut(s) 930
PflMI CCANNNNNTGG 2 cut(s) 222, 1000
PkrI GCNGC 3 cut(s) 316, 326, 421
PleI GAGTC 2 cut(s) 128, 816
PpsI GAGTC 2 cut(s) 128, 816
Ppu21I YACGTR 1 cut(s) 662
PpuMI RGGWCCY 1 cut(s) 990
Psp5II RGGWCCY 1 cut(s) 990
Psp6I CCWGG 1 cut(s) 250
PspFI CCCAGC 2 cut(s) 242, 826
PspGI CCWGG 1 cut(s) 250
PspLI CGTACG 1 cut(s) 930
PspN4I GGNNCC 3 cut(s) 256, 757, 992
PspPI GGNCC 3 cut(s) 255, 756, 990
PspPPI RGGWCCY 1 cut(s) 990
RsaI GTAC 3 cut(s) 625, 664, 932
RsaNI GTAC 3 cut(s) 624, 663, 931
RseI CAYNNNNRTG 2 cut(s) 81, 129
SaqAI TTAA 2 cut(s) 500, 602
SatI GCNGC 3 cut(s) 315, 325, 420
Sau3AI GATC 2 cut(s) 14, 633
Sau96I GGNCC 3 cut(s) 255, 756, 990
SchI GAGTC 2 cut(s) 129, 816
ScrFI CCNGG 3 cut(s) 252, 269, 577
Sfr274I CTCGAG 1 cut(s) 517
SinI GGWCC 1 cut(s) 990
SlaI CTCGAG 1 cut(s) 517
SmiMI CAYNNNNRTG 2 cut(s) 81, 129
SmlI CTYRAG 1 cut(s) 517
SmoI CTYRAG 1 cut(s) 517
SphI GCATGC 1 cut(s) 950
Sse9I AATT 6 cut(s) 102, 153, 209, 385, 874, 998
SseBI AGGCCT 1 cut(s) 46
SspMI CTAG 4 cut(s) 162, 189, 900, 987
StuI AGGCCT 1 cut(s) 46
StyD4I CCNGG 3 cut(s) 250, 267, 575
StyI CCWWGG 1 cut(s) 986
TaaI ACNGT 6 cut(s) 392, 410, 542, 628, 741, 925
TaiI ACGT 2 cut(s) 664, 932
TaqI TCGA 2 cut(s) 518, 918
TasI AATT 6 cut(s) 102, 153, 209, 385, 874, 998
TfiI GAWTC 1 cut(s) 297
Tru1I TTAA 2 cut(s) 500, 602
Tru9I TTAA 2 cut(s) 500, 602
TscAI CASTG 2 cut(s) 547, 750
TseFI GTSAC 3 cut(s) 536, 568, 741
TseI GCWGC 3 cut(s) 314, 324, 419
Tsp45I GTSAC 3 cut(s) 536, 568, 741
TspDTI ATGAA 4 cut(s) 275, 517, 603, 1012
TspGWI ACGGA 1 cut(s) 762
TspRI CASTG 2 cut(s) 547, 750
Van91I CCANNNNNTGG 2 cut(s) 222, 1000
VpaK11BI GGWCC 1 cut(s) 990
XapI RAATTY 2 cut(s) 209, 874
XceI RCATGY 2 cut(s) 683, 950
XcmI CCANNNNNNNNNTGG 1 cut(s) 644
XhoI CTCGAG 1 cut(s) 517
XmaJI CCTAGG 1 cut(s) 986
XspI CTAG 4 cut(s) 162, 189, 900, 987
Zsp2I ATGCAT 1 cut(s) 952
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.