MD09G1086100.v1.1

carboxylesterase 17

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0009064
Physical Location & Seq
Forward (+)
6156239 .. 6157270
1032 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1086100.v1.1.491

Sequence Viewer

Length: 1032 bp
ATGGCAGCCATATCTCTTGATCCAAGGCTTAACCTCCAAGCAACTAAAAACCCTAACCAACATGGAGTTATACTTGAAGAAATCGAAGGCCTCATCCGAGTTCACAAAGACGGATACGTCGAAAGGCCTCTGATGATCCCAAGTGTCCCCAGCACTCTAGCTCTACCACCTGGTGTCGAGGCCAAAGATATTGTTATTGACAAATTTACCAACTTATGGGCACGCATTTATGTTCCAAGCCACCCGGGAAACCTCCCCGTGATTGTCTACTTCCACGGCGGTGGATTCTGTGTTGGATCTGCTGCTTGGAGCTGTTACCATGAGTTCCTGGCCAAACTTGCTTCACAAGCAAGTTGTGTCATCATCTCTGTGAATTACCGTTTAGCCCCTGAAAGCCGTCTCCCTGCTGCTTATGATGACGGATTCAAAACCCTTATGTGGGTAAAACAAAAAGCTCTGAGTGAGTCTAGTGAGCAAAAATGGTGGTTAAGTAAGTGCAACTTGTCCAGCTTGTTCATAGGCGGTGACAGTGCAGGGGCTAACATAGCCTACAATGTCACCACACAGGTAGGTTCACGTGACCCCTCCATTTTAAGGCCATTAAGTCTCAAAGGCACCGTCTTGATCCAACCCTTTTTTGGAGGAGAGGCTCGAACTTGGTCCGAAAAATGTGCAACTCAGCCACCATACTCCGCCCTAAGTCTGTCAAGTTCAGACACATATTGGCGGCTGTCTCTTCCGTTAGGGGCCAGCAGAGATCATCCGTGGTGCAACCCTCTAGTGAACGCCGGTGTGGCCAAATTACGCGATTTAAGGCTCCCGAGCATGATGGTATGCGTATCAGAATTGGATATATTGAAGGATGGGAATTTTGAGTTGTGCAACGCATTGAGTAGCATTGGGACGAGGGTGGAAACTGTACTGTACAAAGGAGTTGGACATGCATTCCAAGTTCTGCATAACTCTCAGCTATCTTATTCACGGACACAAGAATTGATATCTCGCATCAAGGCCTTCATGATCAAGCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

344

Amino Acids

37.77

Weight (kDa)

8.7

Isoelectric Point (pI)

38.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BD-FAE PF20434 76 - 184 1.5e-10 BD-FAE
COesterase PF00135 76 - 130 1.3e-08 Carboxylesterase family
Abhydrolase_3 PF07859 88 - 317 2.1e-56 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 116
AccB1I GGYRCC 1 cut(s) 614
AccB7I CCANNNNNTGG 1 cut(s) 216
AccI GTMKAC 1 cut(s) 267
AccII CGCG 1 cut(s) 807
AciI CCGC 4 cut(s) 279, 522, 693, 727
AclWI GGATC 4 cut(s) 14, 130, 304, 619
AcoI YGGCCR 2 cut(s) 330, 795
AcsI RAATTY 2 cut(s) 203, 868
AcvI CACGTG 1 cut(s) 578
AdeI CACNNNGTG 1 cut(s) 173
AfaI GTAC 2 cut(s) 921, 926
AfiI CCNNNNNNNGG 5 cut(s) 216, 438, 439, 594, 638
AgsI TTSAA 3 cut(s) 77, 427, 859
AjnI CCWGG 2 cut(s) 169, 327
AleI CACNNNNGTG 1 cut(s) 279
AluBI AGCT 5 cut(s) 161, 312, 455, 510, 970
AluI AGCT 5 cut(s) 161, 312, 455, 510, 970
Alw26I GTCTC 3 cut(s) 404, 611, 738
AlwI GGATC 4 cut(s) 14, 130, 304, 619
Ama87I CYCGRG 2 cut(s) 244, 820
AoxI GGCC 8 cut(s) 88, 125, 180, 330, 596, 747, 795, 1011
ApeKI GCWGC 3 cut(s) 5, 302, 407
ApoI RAATTY 2 cut(s) 203, 868
ArsI GACNNNNNNTTYG 2 cut(s) 603, 635
AspS9I GGNCC 2 cut(s) 660, 747
AsuC2I CCSGG 2 cut(s) 245, 246
AsuHPI GGTGA 2 cut(s) 536, 550
AvaI CYCGRG 2 cut(s) 244, 820
AvaII GGWCC 1 cut(s) 660
BaeGI GKGCMC 1 cut(s) 223
BalI TGGCCA 2 cut(s) 332, 797
BanI GGYRCC 1 cut(s) 614
BbrPI CACGTG 1 cut(s) 578
BbvI GCAGC 3 cut(s) 17, 289, 394
BccI CCATC 2 cut(s) 823, 857
BceAI ACGGC 2 cut(s) 292, 381
BcgI CGANNNNNNTGC 2 cut(s) 653, 687
BciT130I CCWGG 2 cut(s) 171, 329
BciVI GTATCC 1 cut(s) 107
BclI TGATCA 1 cut(s) 1020
BcnI CCSGG 2 cut(s) 245, 246
BcoDI GTCTC 3 cut(s) 404, 611, 738
BfaI CTAG 3 cut(s) 158, 468, 779
BfuI GTATCC 1 cut(s) 107
BglI GCCNNNNNGGC 1 cut(s) 794
BisI GCNGC 4 cut(s) 6, 303, 408, 728
BlsI GCNGC 4 cut(s) 7, 304, 409, 729
Bme1390I CCNGG 4 cut(s) 171, 245, 246, 329
Bme18I GGWCC 1 cut(s) 660
BmeT110I CYCGRG 2 cut(s) 244, 820
BmgT120I GGNCC 2 cut(s) 660, 747
BmiI GGNNCC 3 cut(s) 616, 748, 818
BmrFI CCNGG 4 cut(s) 171, 245, 246, 329
BmsI GCATC 1 cut(s) 1014
BpuMI CCSGG 2 cut(s) 245, 246
BsaAI YACGTR 1 cut(s) 578
BsaJI CCNNGG 4 cut(s) 23, 244, 274, 764
Bsc4I CCNNNNNNNGG 5 cut(s) 216, 438, 439, 594, 638
Bse118I RCCGGY 1 cut(s) 788
BseBI CCWGG 2 cut(s) 171, 329
BseDI CCNNGG 4 cut(s) 23, 244, 274, 764
BseGI GGATG 3 cut(s) 93, 760, 868
BseLI CCNNNNNNNGG 5 cut(s) 216, 438, 439, 594, 638
BseMII CTCAG 3 cut(s) 449, 692, 980
BseRI GAGGAG 1 cut(s) 657
BseSI GKGCMC 1 cut(s) 223
BseXI GCAGC 3 cut(s) 17, 289, 394
BseYI CCCAGC 1 cut(s) 149
BsgI GTGCAG 1 cut(s) 552
Bsh1236I CGCG 1 cut(s) 807
BshFI GGCC 8 cut(s) 90, 127, 182, 332, 598, 749, 797, 1013
BshNI GGYRCC 1 cut(s) 614
BsiHKCI CYCGRG 2 cut(s) 244, 820
BsiSI CCGG 2 cut(s) 245, 789
BslFI GGGAC 2 cut(s) 131, 916
BslI CCNNNNNNNGG 5 cut(s) 216, 438, 439, 594, 638
BsmAI GTCTC 3 cut(s) 404, 611, 738
BsmBI CGTCTC 1 cut(s) 404
BsmFI GGGAC 2 cut(s) 131, 916
BsmI GAATGC 1 cut(s) 944
BsnI GGCC 8 cut(s) 90, 127, 182, 332, 598, 749, 797, 1013
BsoBI CYCGRG 2 cut(s) 244, 820
Bsp1286I GDGCHC 1 cut(s) 223
Bsp1407I TGTACA 1 cut(s) 924
Bsp143I GATC 6 cut(s) 19, 135, 296, 624, 757, 1020
BspACI CCGC 4 cut(s) 279, 522, 693, 727
BspANI GGCC 8 cut(s) 90, 127, 182, 332, 598, 749, 797, 1013
BspCNI CTCAG 3 cut(s) 450, 691, 979
BspFNI CGCG 1 cut(s) 807
BspHI TCATGA 1 cut(s) 1017
BspLI GGNNCC 3 cut(s) 616, 748, 818
BspPI GGATC 4 cut(s) 14, 130, 304, 619
BspT107I GGYRCC 1 cut(s) 614
BsrFI RCCGGY 1 cut(s) 788
BsrGI TGTACA 1 cut(s) 924
BssAI RCCGGY 1 cut(s) 788
BssECI CCNNGG 4 cut(s) 23, 244, 274, 764
BssMI GATC 6 cut(s) 19, 135, 296, 624, 757, 1020
BssT1I CCWWGG 1 cut(s) 23
Bst2UI CCWGG 2 cut(s) 171, 329
Bst4CI ACNGT 5 cut(s) 380, 530, 619, 919, 924
Bst6I CTCTTC 1 cut(s) 741
BstAUI TGTACA 1 cut(s) 924
BstBAI YACGTR 1 cut(s) 578
BstC8I GCNNGC 2 cut(s) 223, 751
BstDEI CTNAG 4 cut(s) 458, 678, 698, 966
BstDSI CCRYGG 2 cut(s) 274, 764
BstF5I GGATG 3 cut(s) 93, 760, 868
BstFNI CGCG 1 cut(s) 807
BstKTI GATC 6 cut(s) 22, 138, 299, 627, 760, 1023
BstMAI GTCTC 3 cut(s) 404, 611, 738
BstMBI GATC 6 cut(s) 19, 135, 296, 624, 757, 1020
BstMWI GCNNNNNNNGC 4 cut(s) 338, 347, 545, 794
BstNI CCWGG 2 cut(s) 171, 329
BstNSI RCATGY 1 cut(s) 944
BstSCI CCNGG 4 cut(s) 169, 243, 244, 327
BstSLI GKGCMC 1 cut(s) 223
BstUI CGCG 1 cut(s) 807
BstV1I GCAGC 3 cut(s) 17, 289, 394
BstX2I RGATCY 1 cut(s) 296
BstXI CCANNNNNNTGG 1 cut(s) 281
BstYI RGATCY 1 cut(s) 296
BsuI GTATCC 1 cut(s) 107
BsuRI GGCC 8 cut(s) 90, 127, 182, 332, 598, 749, 797, 1013
BtgI CCRYGG 2 cut(s) 274, 764
BtsCI GGATG 3 cut(s) 93, 760, 868
BtsIMutI CAGTG 1 cut(s) 535
Cac8I GCNNGC 2 cut(s) 223, 751
CciI TCATGA 1 cut(s) 1017
Cfr10I RCCGGY 1 cut(s) 788
Cfr13I GGNCC 2 cut(s) 660, 747
Cfr9I CCCGGG 1 cut(s) 244
CsiI ACCWGGT 1 cut(s) 169
Csp6I GTAC 2 cut(s) 920, 925
CspCI CAANNNNNGTGG 2 cut(s) 464, 499
CviAII CATG 5 cut(s) 62, 320, 826, 941, 1018
CviQI GTAC 2 cut(s) 920, 925
DdeI CTNAG 4 cut(s) 458, 678, 698, 966
DpnI GATC 6 cut(s) 21, 137, 298, 626, 759, 1022
DpnII GATC 6 cut(s) 19, 135, 296, 624, 757, 1020
DraIII CACNNNGTG 1 cut(s) 173
DrdI GACNNNNNNGTC 1 cut(s) 116
DseDI GACNNNNNNGTC 1 cut(s) 116
EaeI YGGCCR 2 cut(s) 330, 795
Eam1104I CTCTTC 1 cut(s) 741
EarI CTCTTC 1 cut(s) 741
EciI GGCGGA 1 cut(s) 682
Eco130I CCWWGG 1 cut(s) 23
Eco147I AGGCCT 3 cut(s) 90, 127, 1013
Eco32I GATATC 1 cut(s) 999
Eco47I GGWCC 1 cut(s) 660
Eco72I CACGTG 1 cut(s) 578
Eco88I CYCGRG 2 cut(s) 244, 820
EcoRII CCWGG 2 cut(s) 169, 327
EcoRV GATATC 1 cut(s) 999
EcoT14I CCWWGG 1 cut(s) 23
EcoT22I ATGCAT 1 cut(s) 946
ErhI CCWWGG 1 cut(s) 23
Esp3I CGTCTC 1 cut(s) 404
FaeI CATG 5 cut(s) 65, 323, 829, 944, 1021
FalI AAGNNNNNCTT 2 cut(s) 485, 517
FaqI GGGAC 2 cut(s) 131, 916
FatI CATG 5 cut(s) 61, 319, 825, 940, 1017
FbaI TGATCA 1 cut(s) 1020
FblI GTMKAC 1 cut(s) 267
Fnu4HI GCNGC 4 cut(s) 6, 303, 408, 728
FokI GGATG 3 cut(s) 80, 747, 875
Fsp4HI GCNGC 4 cut(s) 6, 303, 408, 728
FspBI CTAG 3 cut(s) 158, 468, 779
GluI GCNGC 4 cut(s) 6, 303, 408, 728
GsaI CCCAGC 1 cut(s) 153
HaeIII GGCC 8 cut(s) 90, 127, 182, 332, 598, 749, 797, 1013
HapII CCGG 2 cut(s) 245, 789
Hin1II CATG 5 cut(s) 65, 323, 829, 944, 1021
HinfI GANTC 3 cut(s) 285, 423, 464
HpaII CCGG 2 cut(s) 245, 789
HphI GGTGA 2 cut(s) 536, 550
Hpy166II GTNNAC 4 cut(s) 103, 268, 575, 784
Hpy188I TCNGA 6 cut(s) 98, 132, 459, 664, 715, 844
Hpy188III TCNNGA 4 cut(s) 17, 622, 820, 1018
Hpy8I GTNNAC 4 cut(s) 103, 268, 575, 784
Hpy99I CGWCG 1 cut(s) 122
HpyAV CCTTC 3 cut(s) 80, 853, 1024
HpyCH4III ACNGT 5 cut(s) 380, 530, 619, 919, 924
HpyCH4IV ACGT 2 cut(s) 117, 577
HpyCH4V TGCA 7 cut(s) 498, 533, 674, 771, 882, 944, 958
HpyF10VI GCNNNNNNNGC 4 cut(s) 338, 347, 545, 794
HpyF3I CTNAG 4 cut(s) 458, 678, 698, 966
HpySE526I ACGT 2 cut(s) 117, 577
Hsp92II CATG 5 cut(s) 65, 323, 829, 944, 1021
Ksp22I TGATCA 1 cut(s) 1020
Kzo9I GATC 6 cut(s) 19, 135, 296, 624, 757, 1020
LmnI GCTCC 2 cut(s) 309, 822
Lsp1109I GCAGC 3 cut(s) 17, 289, 394
LweI GCATC 1 cut(s) 1014
MabI ACCWGGT 1 cut(s) 169
MaeI CTAG 3 cut(s) 158, 468, 779
MaeII ACGT 2 cut(s) 117, 577
MaeIII GTNAC 4 cut(s) 314, 524, 556, 578
MalI GATC 6 cut(s) 21, 137, 298, 626, 759, 1022
MboI GATC 6 cut(s) 19, 135, 296, 624, 757, 1020
MboII GAAGA 2 cut(s) 89, 728
MflI RGATCY 1 cut(s) 296
MhlI GDGCHC 1 cut(s) 223
MlsI TGGCCA 2 cut(s) 332, 797
MluCI AATT 6 cut(s) 203, 373, 800, 845, 868, 992
MluNI TGGCCA 2 cut(s) 332, 797
MlyI GAGTC 1 cut(s) 473
MmeI TCCRAC 3 cut(s) 274, 652, 916
Mox20I TGGCCA 2 cut(s) 332, 797
Mph1103I ATGCAT 1 cut(s) 946
MscI TGGCCA 2 cut(s) 332, 797
MseI TTAA 5 cut(s) 30, 488, 593, 602, 812
MslI CAYNNNNRTG 2 cut(s) 279, 368
Msp20I TGGCCA 2 cut(s) 332, 797
MspI CCGG 2 cut(s) 245, 789
MspR9I CCNGG 4 cut(s) 171, 245, 246, 329
Mva1269I GAATGC 1 cut(s) 944
MvaI CCWGG 2 cut(s) 171, 329
MvnI CGCG 1 cut(s) 807
MwoI GCNNNNNNNGC 4 cut(s) 338, 347, 545, 794
NciI CCSGG 2 cut(s) 245, 246
NdeII GATC 6 cut(s) 19, 135, 296, 624, 757, 1020
NlaIII CATG 5 cut(s) 65, 323, 829, 944, 1021
NlaIV GGNNCC 3 cut(s) 616, 748, 818
NmuCI GTSAC 3 cut(s) 524, 556, 578
NsiI ATGCAT 1 cut(s) 946
NspI RCATGY 1 cut(s) 944
OliI CACNNNNGTG 1 cut(s) 279
PagI TCATGA 1 cut(s) 1017
PceI AGGCCT 3 cut(s) 90, 127, 1013
PcsI WCGNNNNNNNCGW 1 cut(s) 117
PctI GAATGC 1 cut(s) 944
PfeI GAWTC 2 cut(s) 285, 423
PflMI CCANNNNNTGG 1 cut(s) 216
PkrI GCNGC 4 cut(s) 7, 304, 409, 729
PleI GAGTC 1 cut(s) 472
PmaCI CACGTG 1 cut(s) 578
PmlI CACGTG 1 cut(s) 578
PpsI GAGTC 1 cut(s) 472
Ppu21I YACGTR 1 cut(s) 578
Psp6I CCWGG 2 cut(s) 169, 327
PspCI CACGTG 1 cut(s) 578
PspFI CCCAGC 1 cut(s) 149
PspGI CCWGG 2 cut(s) 169, 327
PspN4I GGNNCC 3 cut(s) 616, 748, 818
PspPI GGNCC 2 cut(s) 660, 747
PsuI RGATCY 1 cut(s) 296
RsaI GTAC 2 cut(s) 921, 926
RsaNI GTAC 2 cut(s) 920, 925
RseI CAYNNNNRTG 2 cut(s) 279, 368
SaqAI TTAA 5 cut(s) 30, 488, 593, 602, 812
SatI GCNGC 4 cut(s) 6, 303, 408, 728
Sau3AI GATC 6 cut(s) 19, 135, 296, 624, 757, 1020
Sau96I GGNCC 2 cut(s) 660, 747
SchI GAGTC 1 cut(s) 473
ScrFI CCNGG 4 cut(s) 171, 245, 246, 329
SduI GDGCHC 1 cut(s) 223
SexAI ACCWGGT 1 cut(s) 169
SfaNI GCATC 1 cut(s) 1014
SgrAI CRCCGGYG 1 cut(s) 788
SinI GGWCC 1 cut(s) 660
SmaI CCCGGG 1 cut(s) 246
SmiMI CAYNNNNRTG 2 cut(s) 279, 368
Sse9I AATT 6 cut(s) 203, 373, 800, 845, 868, 992
SseBI AGGCCT 3 cut(s) 90, 127, 1013
SsiI CCGC 4 cut(s) 279, 522, 693, 727
SspMI CTAG 3 cut(s) 158, 468, 779
StuI AGGCCT 3 cut(s) 90, 127, 1013
StyD4I CCNGG 4 cut(s) 169, 243, 244, 327
StyI CCWWGG 1 cut(s) 23
TaaI ACNGT 5 cut(s) 380, 530, 619, 919, 924
TaiI ACGT 2 cut(s) 120, 580
TaqI TCGA 4 cut(s) 84, 120, 177, 652
TasI AATT 6 cut(s) 203, 373, 800, 845, 868, 992
TatI WGTACW 2 cut(s) 919, 924
TauI GCSGC 1 cut(s) 730
TfiI GAWTC 2 cut(s) 285, 423
Tru1I TTAA 5 cut(s) 30, 488, 593, 602, 812
Tru9I TTAA 5 cut(s) 30, 488, 593, 602, 812
TscAI CASTG 1 cut(s) 535
TseFI GTSAC 3 cut(s) 524, 556, 578
TseI GCWGC 3 cut(s) 5, 302, 407
Tsp45I GTSAC 3 cut(s) 524, 556, 578
TspDTI ATGAA 2 cut(s) 505, 1006
TspGWI ACGGA 5 cut(s) 126, 435, 729, 753, 997
TspMI CCCGGG 1 cut(s) 244
TspRI CASTG 1 cut(s) 535
Van91I CCANNNNNTGG 1 cut(s) 216
VpaK11BI GGWCC 1 cut(s) 660
XapI RAATTY 2 cut(s) 203, 868
XceI RCATGY 1 cut(s) 944
XcmI CCANNNNNNNNNTGG 1 cut(s) 635
XmaI CCCGGG 1 cut(s) 244
XmiI GTMKAC 1 cut(s) 267
XspI CTAG 3 cut(s) 158, 468, 779
Zsp2I ATGCAT 1 cut(s) 946
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.