pycom09g01140

carboxylesterase 17

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0009064
Physical Location & Seq
Forward (+)
895315 .. 896346
1032 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g01140.1

Sequence Viewer

Length: 1032 bp
ATGGCAGCCATATCTCTTGATCCAAGGCTTAACCTTCAAGCAACTAAAAACCCTAACCAACATGGAGTTATACTCGAAGAAATCGAAGGCCTCATCCGAGTTCACAAAGACGGATACGTCGAAAGGCCTCTGATGATCCCAAGTGTCCCCAGCACTCTAGCTCTACCACCTGGTGTTACGGCCAAAGACGTTGTCATTGACAAATTTACCAATTTGTGGGCACGCATTTATGTTCCAAGCCACCCGGGAAACCTCCCCGTGATTGTCTACTTCCATGGCGGCGGATTTTGTGTTGGATCTGCTTCTTGGAGCTGTTACCATGAGTTCCTAGCCAAACTTGCTTCACAAGCAAGTTGTGTCATCATCTCTGTGAATTACCGTTTAGCCCCTGAAAGCCGTCTCCCTGCTGCTTATGATGACGGATTCAAAACCCTTATGTGGGTAAAACAACAAGCTCTGAGTGAGTCTACTGAGCAAAAATGGTGGTTAAGTAAGTGCAACTTGTCCAGCTTGTTCATAGGTGGTGATAGTGCAGGGGCTAACATAGCCTACAATGTCACCACACAGGTAGGTTCATGTGACCCCTCCATTTTAAGGCCATTGAGTCTCAAAGGCACCGTATTGATCCAACCCTTTTTCGGAGGAGAGGCTCGAACTTGGTCCGAAAAATGTGCAACTCAGCCACCATACTCCGCCCTAAGTCTGTCAAGTTCAGACACATATTGGCGGCTGTCTCTTCCGTTAGGGGCCAGCAGAGACCATCCGTGGTGCAACCCTCTAGTGAACGCCGGCGTGGCCAAATTGCGCGATTTAAGGCTCCCAAGCATGATGGTATGCGTATCAGAATTGGATATATTGAAGGATAGGAATTTTGAGTTGTGCAACGCATTGAGTAGCATTGGGACAAGGGTGGAAACTGTACTGTACAAAGGAGTTGGCCATGCATTCCAAGTTCTGCAAAACTCTCAGCTCTCTTATTCACGGACACAAGAATTGATATCTCACATCAAGGCCTTCATGATCAAGCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

344

Amino Acids

37.77

Weight (kDa)

8.51

Isoelectric Point (pI)

40.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BD-FAE PF20434 76 - 184 1.4e-10 BD-FAE
COesterase PF00135 76 - 130 4.6e-09 Carboxylesterase family
Abhydrolase_3 PF07859 88 - 317 1.4e-56 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 116
AccB1I GGYRCC 1 cut(s) 614
AccB7I CCANNNNNTGG 1 cut(s) 216
AccI GTMKAC 2 cut(s) 267, 467
AccII CGCG 1 cut(s) 807
AciI CCGC 4 cut(s) 279, 282, 693, 727
AclWI GGATC 4 cut(s) 14, 130, 304, 619
AcoI YGGCCR 3 cut(s) 180, 795, 937
AcsI RAATTY 2 cut(s) 203, 868
AdeI CACNNNGTG 1 cut(s) 173
AfaI GTAC 2 cut(s) 921, 926
AfiI CCNNNNNNNGG 5 cut(s) 216, 438, 439, 594, 638
AgsI TTSAA 3 cut(s) 38, 427, 859
AjnI CCWGG 1 cut(s) 169
AluBI AGCT 5 cut(s) 161, 312, 455, 510, 970
AluI AGCT 5 cut(s) 161, 312, 455, 510, 970
Alw26I GTCTC 4 cut(s) 404, 611, 738, 750
AlwI GGATC 4 cut(s) 14, 130, 304, 619
Ama87I CYCGRG 1 cut(s) 244
AoxI GGCC 8 cut(s) 88, 125, 180, 596, 747, 795, 937, 1011
ApeKI GCWGC 2 cut(s) 5, 407
ApoI RAATTY 2 cut(s) 203, 868
ArsI GACNNNNNNTTYG 2 cut(s) 177, 209
AspLEI GCGC 1 cut(s) 807
AspS9I GGNCC 2 cut(s) 660, 747
AsuC2I CCSGG 2 cut(s) 245, 246
AsuHPI GGTGA 2 cut(s) 536, 550
AvaI CYCGRG 1 cut(s) 244
AvaII GGWCC 1 cut(s) 660
BaeGI GKGCMC 1 cut(s) 223
BalI TGGCCA 2 cut(s) 797, 939
BanI GGYRCC 1 cut(s) 614
BbvI GCAGC 2 cut(s) 17, 394
BccI CCATC 2 cut(s) 768, 823
BceAI ACGGC 2 cut(s) 195, 381
BcgI CGANNNNNNTGC 2 cut(s) 653, 687
BciT130I CCWGG 1 cut(s) 171
BciVI GTATCC 1 cut(s) 107
BclI TGATCA 1 cut(s) 1020
BcnI CCSGG 2 cut(s) 245, 246
BcoDI GTCTC 4 cut(s) 404, 611, 738, 750
BfaI CTAG 3 cut(s) 158, 329, 779
BfuI GTATCC 1 cut(s) 107
BglI GCCNNNNNGGC 1 cut(s) 794
BisI GCNGC 4 cut(s) 6, 280, 408, 728
BlsI GCNGC 4 cut(s) 7, 281, 409, 729
Bme1390I CCNGG 3 cut(s) 171, 245, 246
Bme18I GGWCC 1 cut(s) 660
BmeT110I CYCGRG 1 cut(s) 244
BmgT120I GGNCC 2 cut(s) 660, 747
BmiI GGNNCC 3 cut(s) 616, 748, 818
BmrFI CCNGG 3 cut(s) 171, 245, 246
BplI GAGNNNNNCTC 2 cut(s) 57, 89
BpuMI CCSGG 2 cut(s) 245, 246
BsaI GGTCTC 1 cut(s) 750
BsaJI CCNNGG 4 cut(s) 23, 244, 274, 764
Bsc4I CCNNNNNNNGG 5 cut(s) 216, 438, 439, 594, 638
Bse118I RCCGGY 1 cut(s) 788
BseBI CCWGG 1 cut(s) 171
BseDI CCNNGG 4 cut(s) 23, 244, 274, 764
BseGI GGATG 2 cut(s) 93, 760
BseLI CCNNNNNNNGG 5 cut(s) 216, 438, 439, 594, 638
BseMII CTCAG 4 cut(s) 449, 462, 692, 980
BseRI GAGGAG 1 cut(s) 657
BseSI GKGCMC 1 cut(s) 223
BseXI GCAGC 2 cut(s) 17, 394
BseYI CCCAGC 1 cut(s) 149
BsgI GTGCAG 1 cut(s) 552
Bsh1236I CGCG 1 cut(s) 807
BshFI GGCC 8 cut(s) 90, 127, 182, 598, 749, 797, 939, 1013
BshNI GGYRCC 1 cut(s) 614
BsiHKCI CYCGRG 1 cut(s) 244
BsiSI CCGG 2 cut(s) 245, 789
BslFI GGGAC 2 cut(s) 131, 916
BslI CCNNNNNNNGG 5 cut(s) 216, 438, 439, 594, 638
BsmAI GTCTC 4 cut(s) 404, 611, 738, 750
BsmBI CGTCTC 1 cut(s) 404
BsmFI GGGAC 2 cut(s) 131, 916
BsmI GAATGC 1 cut(s) 944
BsnI GGCC 8 cut(s) 90, 127, 182, 598, 749, 797, 939, 1013
Bso31I GGTCTC 1 cut(s) 750
BsoBI CYCGRG 1 cut(s) 244
Bsp1286I GDGCHC 1 cut(s) 223
Bsp1407I TGTACA 1 cut(s) 924
Bsp143I GATC 5 cut(s) 19, 135, 296, 624, 1020
Bsp19I CCATGG 1 cut(s) 274
BspACI CCGC 4 cut(s) 279, 282, 693, 727
BspANI GGCC 8 cut(s) 90, 127, 182, 598, 749, 797, 939, 1013
BspCNI CTCAG 4 cut(s) 450, 463, 691, 979
BspFNI CGCG 1 cut(s) 807
BspHI TCATGA 1 cut(s) 1017
BspLI GGNNCC 3 cut(s) 616, 748, 818
BspPI GGATC 4 cut(s) 14, 130, 304, 619
BspT107I GGYRCC 1 cut(s) 614
BspTNI GGTCTC 1 cut(s) 750
BsrFI RCCGGY 1 cut(s) 788
BsrGI TGTACA 1 cut(s) 924
BssAI RCCGGY 1 cut(s) 788
BssECI CCNNGG 4 cut(s) 23, 244, 274, 764
BssMI GATC 5 cut(s) 19, 135, 296, 624, 1020
BssT1I CCWWGG 2 cut(s) 23, 274
Bst2UI CCWGG 1 cut(s) 171
Bst4CI ACNGT 4 cut(s) 380, 619, 919, 924
Bst6I CTCTTC 1 cut(s) 741
BstAUI TGTACA 1 cut(s) 924
BstC8I GCNNGC 3 cut(s) 223, 751, 790
BstDEI CTNAG 5 cut(s) 458, 471, 678, 698, 966
BstDSI CCRYGG 2 cut(s) 274, 764
BstF5I GGATG 2 cut(s) 93, 760
BstFNI CGCG 1 cut(s) 807
BstHHI GCGC 1 cut(s) 807
BstKTI GATC 5 cut(s) 22, 138, 299, 627, 1023
BstMAI GTCTC 4 cut(s) 404, 611, 738, 750
BstMBI GATC 5 cut(s) 19, 135, 296, 624, 1020
BstMWI GCNNNNNNNGC 4 cut(s) 338, 347, 545, 794
BstNI CCWGG 1 cut(s) 171
BstSCI CCNGG 3 cut(s) 169, 243, 244
BstSLI GKGCMC 1 cut(s) 223
BstUI CGCG 1 cut(s) 807
BstV1I GCAGC 2 cut(s) 17, 394
BstX2I RGATCY 1 cut(s) 296
BstYI RGATCY 1 cut(s) 296
BsuI GTATCC 1 cut(s) 107
BsuRI GGCC 8 cut(s) 90, 127, 182, 598, 749, 797, 939, 1013
BtgI CCRYGG 2 cut(s) 274, 764
BtsCI GGATG 2 cut(s) 93, 760
Cac8I GCNNGC 3 cut(s) 223, 751, 790
CciI TCATGA 1 cut(s) 1017
CfoI GCGC 1 cut(s) 807
Cfr10I RCCGGY 1 cut(s) 788
Cfr13I GGNCC 2 cut(s) 660, 747
Cfr9I CCCGGG 1 cut(s) 244
CsiI ACCWGGT 1 cut(s) 169
Csp6I GTAC 2 cut(s) 920, 925
CspCI CAANNNNNGTGG 2 cut(s) 464, 499
CviAII CATG 7 cut(s) 62, 275, 320, 576, 826, 941, 1018
CviQI GTAC 2 cut(s) 920, 925
DdeI CTNAG 5 cut(s) 458, 471, 678, 698, 966
DpnI GATC 5 cut(s) 21, 137, 298, 626, 1022
DpnII GATC 5 cut(s) 19, 135, 296, 624, 1020
DraIII CACNNNGTG 1 cut(s) 173
DrdI GACNNNNNNGTC 1 cut(s) 116
DseDI GACNNNNNNGTC 1 cut(s) 116
EaeI YGGCCR 3 cut(s) 180, 795, 937
Eam1104I CTCTTC 1 cut(s) 741
EarI CTCTTC 1 cut(s) 741
EciI GGCGGA 2 cut(s) 297, 682
Eco130I CCWWGG 2 cut(s) 23, 274
Eco147I AGGCCT 3 cut(s) 90, 127, 1013
Eco31I GGTCTC 1 cut(s) 750
Eco32I GATATC 1 cut(s) 999
Eco47I GGWCC 1 cut(s) 660
Eco88I CYCGRG 1 cut(s) 244
EcoRII CCWGG 1 cut(s) 169
EcoRV GATATC 1 cut(s) 999
EcoT14I CCWWGG 2 cut(s) 23, 274
EcoT22I ATGCAT 1 cut(s) 946
ErhI CCWWGG 2 cut(s) 23, 274
Esp3I CGTCTC 1 cut(s) 404
FaeI CATG 7 cut(s) 65, 278, 323, 579, 829, 944, 1021
FalI AAGNNNNNCTT 2 cut(s) 485, 517
FaqI GGGAC 2 cut(s) 131, 916
FatI CATG 7 cut(s) 61, 274, 319, 575, 825, 940, 1017
FbaI TGATCA 1 cut(s) 1020
FblI GTMKAC 2 cut(s) 267, 467
Fnu4HI GCNGC 4 cut(s) 6, 280, 408, 728
FokI GGATG 2 cut(s) 80, 747
Fsp4HI GCNGC 4 cut(s) 6, 280, 408, 728
FspBI CTAG 3 cut(s) 158, 329, 779
GlaI GCGC 1 cut(s) 806
GluI GCNGC 4 cut(s) 6, 280, 408, 728
GsaI CCCAGC 1 cut(s) 153
HaeIII GGCC 8 cut(s) 90, 127, 182, 598, 749, 797, 939, 1013
HapII CCGG 2 cut(s) 245, 789
HhaI GCGC 1 cut(s) 807
Hin1II CATG 7 cut(s) 65, 278, 323, 579, 829, 944, 1021
Hin6I GCGC 1 cut(s) 805
HinP1I GCGC 1 cut(s) 805
HinfI GANTC 3 cut(s) 423, 464, 604
HpaII CCGG 2 cut(s) 245, 789
HphI GGTGA 2 cut(s) 536, 550
Hpy166II GTNNAC 4 cut(s) 103, 268, 468, 784
Hpy188I TCNGA 7 cut(s) 98, 132, 459, 641, 664, 715, 844
Hpy188III TCNNGA 2 cut(s) 17, 1018
Hpy8I GTNNAC 4 cut(s) 103, 268, 468, 784
Hpy99I CGWCG 1 cut(s) 122
HpyAV CCTTC 4 cut(s) 44, 80, 853, 1024
HpyCH4III ACNGT 4 cut(s) 380, 619, 919, 924
HpyCH4IV ACGT 2 cut(s) 117, 189
HpyCH4V TGCA 7 cut(s) 498, 533, 674, 771, 882, 944, 958
HpyF10VI GCNNNNNNNGC 4 cut(s) 338, 347, 545, 794
HpyF3I CTNAG 5 cut(s) 458, 471, 678, 698, 966
HpySE526I ACGT 2 cut(s) 117, 189
Hsp92II CATG 7 cut(s) 65, 278, 323, 579, 829, 944, 1021
HspAI GCGC 1 cut(s) 805
KroI GCCGGC 1 cut(s) 788
KroNI GCCGGC 1 cut(s) 790
Ksp22I TGATCA 1 cut(s) 1020
Kzo9I GATC 5 cut(s) 19, 135, 296, 624, 1020
LmnI GCTCC 2 cut(s) 309, 822
Lsp1109I GCAGC 2 cut(s) 17, 394
MabI ACCWGGT 1 cut(s) 169
MaeI CTAG 3 cut(s) 158, 329, 779
MaeII ACGT 2 cut(s) 117, 189
MaeIII GTNAC 4 cut(s) 175, 314, 556, 578
MalI GATC 5 cut(s) 21, 137, 298, 626, 1022
MboI GATC 5 cut(s) 19, 135, 296, 624, 1020
MboII GAAGA 2 cut(s) 89, 728
MflI RGATCY 1 cut(s) 296
MhlI GDGCHC 1 cut(s) 223
MlsI TGGCCA 2 cut(s) 797, 939
MluCI AATT 7 cut(s) 203, 211, 373, 800, 845, 868, 992
MluNI TGGCCA 2 cut(s) 797, 939
MlyI GAGTC 2 cut(s) 473, 613
MmeI TCCRAC 2 cut(s) 274, 652
MnlI CCTC 7 cut(s) 101, 138, 263, 595, 635, 640, 786
Mox20I TGGCCA 2 cut(s) 797, 939
Mph1103I ATGCAT 1 cut(s) 946
MreI CGCCGGCG 1 cut(s) 788
MroNI GCCGGC 1 cut(s) 788
MscI TGGCCA 2 cut(s) 797, 939
MseI TTAA 4 cut(s) 30, 488, 593, 812
MslI CAYNNNNRTG 1 cut(s) 368
Msp20I TGGCCA 2 cut(s) 797, 939
MspI CCGG 2 cut(s) 245, 789
MspR9I CCNGG 3 cut(s) 171, 245, 246
Mva1269I GAATGC 1 cut(s) 944
MvaI CCWGG 1 cut(s) 171
MvnI CGCG 1 cut(s) 807
MwoI GCNNNNNNNGC 4 cut(s) 338, 347, 545, 794
NaeI GCCGGC 1 cut(s) 790
NciI CCSGG 2 cut(s) 245, 246
NcoI CCATGG 1 cut(s) 274
NdeII GATC 5 cut(s) 19, 135, 296, 624, 1020
NgoMIV GCCGGC 1 cut(s) 788
NlaIII CATG 7 cut(s) 65, 278, 323, 579, 829, 944, 1021
NlaIV GGNNCC 3 cut(s) 616, 748, 818
NmuCI GTSAC 2 cut(s) 556, 578
NsiI ATGCAT 1 cut(s) 946
PagI TCATGA 1 cut(s) 1017
PceI AGGCCT 3 cut(s) 90, 127, 1013
PcsI WCGNNNNNNNCGW 2 cut(s) 81, 117
PctI GAATGC 1 cut(s) 944
PdiI GCCGGC 1 cut(s) 790
PfeI GAWTC 1 cut(s) 423
PflFI GACNNNGTC 1 cut(s) 191
PflMI CCANNNNNTGG 1 cut(s) 216
PkrI GCNGC 4 cut(s) 7, 281, 409, 729
PleI GAGTC 2 cut(s) 472, 612
PpsI GAGTC 2 cut(s) 472, 612
Psp6I CCWGG 1 cut(s) 169
PspFI CCCAGC 1 cut(s) 149
PspGI CCWGG 1 cut(s) 169
PspN4I GGNNCC 3 cut(s) 616, 748, 818
PspPI GGNCC 2 cut(s) 660, 747
PsuI RGATCY 1 cut(s) 296
PsyI GACNNNGTC 1 cut(s) 191
RsaI GTAC 2 cut(s) 921, 926
RsaNI GTAC 2 cut(s) 920, 925
RseI CAYNNNNRTG 1 cut(s) 368
SaqAI TTAA 4 cut(s) 30, 488, 593, 812
SatI GCNGC 4 cut(s) 6, 280, 408, 728
Sau3AI GATC 5 cut(s) 19, 135, 296, 624, 1020
Sau96I GGNCC 2 cut(s) 660, 747
SchI GAGTC 2 cut(s) 473, 613
ScrFI CCNGG 3 cut(s) 171, 245, 246
SduI GDGCHC 1 cut(s) 223
SexAI ACCWGGT 1 cut(s) 169
SgrAI CRCCGGYG 1 cut(s) 788
SinI GGWCC 1 cut(s) 660
SmaI CCCGGG 1 cut(s) 246
SmiMI CAYNNNNRTG 1 cut(s) 368
Sse9I AATT 7 cut(s) 203, 211, 373, 800, 845, 868, 992
SseBI AGGCCT 3 cut(s) 90, 127, 1013
SsiI CCGC 4 cut(s) 279, 282, 693, 727
SspMI CTAG 3 cut(s) 158, 329, 779
StuI AGGCCT 3 cut(s) 90, 127, 1013
StyD4I CCNGG 3 cut(s) 169, 243, 244
StyI CCWWGG 2 cut(s) 23, 274
TaaI ACNGT 4 cut(s) 380, 619, 919, 924
TaiI ACGT 2 cut(s) 120, 192
TaqI TCGA 4 cut(s) 75, 84, 120, 652
TasI AATT 7 cut(s) 203, 211, 373, 800, 845, 868, 992
TatI WGTACW 2 cut(s) 919, 924
TauI GCSGC 2 cut(s) 282, 730
TfiI GAWTC 1 cut(s) 423
Tru1I TTAA 4 cut(s) 30, 488, 593, 812
Tru9I TTAA 4 cut(s) 30, 488, 593, 812
TseFI GTSAC 2 cut(s) 556, 578
TseI GCWGC 2 cut(s) 5, 407
Tsp45I GTSAC 2 cut(s) 556, 578
TspDTI ATGAA 3 cut(s) 505, 564, 1006
TspGWI ACGGA 5 cut(s) 126, 435, 729, 753, 997
TspMI CCCGGG 1 cut(s) 244
Tth111I GACNNNGTC 1 cut(s) 191
Van91I CCANNNNNTGG 1 cut(s) 216
VpaK11BI GGWCC 1 cut(s) 660
XapI RAATTY 2 cut(s) 203, 868
XmaI CCCGGG 1 cut(s) 244
XmiI GTMKAC 2 cut(s) 267, 467
XspI CTAG 3 cut(s) 158, 329, 779
Zsp2I ATGCAT 1 cut(s) 946
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.