AT5G17710

Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0004571
Physical Location & Seq
Reverse (-)
5839163 .. 5841858
2696 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G17710.2

Sequence Viewer

Length: 975 bp
ATGGCCGGTCTACTCAAAACGCCGTCTTTACATCTCACACCAACTCTTCTTCATGCTCCTTCTGTACCCTTTAAGCCCTTTTGCGTATCTTTCGCCGGAGGAAGAAACGTTAGCGTCTCACTCTCTCGCCGAGCTTCTCTCCGGTCTGTCTCTAGCGGCTATCCTCTCCGCTTACTAAACCTCGTACCCTTTGCTTCGGGAGAAGCTGAGACGACGGAGACGGAGGTGGAATCGAACGAACCCGAAGAGACGGATGGTGCTGTTGATGTCGAGAATGAAAATGCCAGTGCTGAGGAGGGTGAAGCTGAGGAAGAAGAAGCAGCTGTTATCACAGCGTTGCTGAAATCCTATAAAGAAGCTTTAGCAGACAACAACGAGGGGAAGATTGCTGAGATAGAAGCATCTTTAAAGTCCATAGAAGATGAAAAATTTCTGCTTGCGGATAAAGTAGCGTCTTTATCCAATGAGTTATCGGTGGAGAGGGATCGGCTTATAAGAATCAGTGCAGATTTCGACAACTTCAGGAAGAGGACTGAGAGGGAAAGGCTAAACCTTGTTTCAAATGCTCAAGGAGAGGTTGTAGAGAATCTTTTGGCTGTTTTGGATAATTTCGAGAGAGCTAAATCCCAAATTAAGGTGGAGACTGAGGGAGAAGAGAAAGTCACTAATAGCTATCAGAGCATATACAAACAGTTTGTAGAAATTCTGGGATCACTTGGTGTTATCCATGTGGAGACAGTCGGCAAGCAGTTTGATCCAATGCTACATGAGGCAATAATGAGAGAAGACTCTGCGGAATACGAAGAGGGTATAGTACTAGAAGAATACAGGAAAGGTTTCTTGCTAGGTGAAAGGCTTTTACGTCCTTCAATGGTGAAAGTATCAGCTGGACCTGGACCAGAAAAGCCCCTTGAAGCTGAAGGAGAAGAAGCCACAGCACAAGGAAGCGCAGAAGAAGAATCATCTTCATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GrpE

Protein Analysis

324

Amino Acids

35.49

Weight (kDa)

4.57

Isoelectric Point (pI)

41.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GrpE PF01025 144 - 297 5.2e-36 GrpE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 494
AccI GTMKAC 1 cut(s) 10
AciI CCGC 4 cut(s) 156, 169, 440, 794
AclI AACGTT 1 cut(s) 108
AclWI GGATC 3 cut(s) 492, 718, 749
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 428, 702
AcuI CTGAAG 2 cut(s) 505, 939
AdeI CACNNNGTG 1 cut(s) 719
AfaI GTAC 3 cut(s) 66, 186, 816
AfiI CCNNNNNNNGG 1 cut(s) 634
AgsI TTSAA 3 cut(s) 561, 870, 914
AjnI CCWGG 1 cut(s) 892
AluBI AGCT 9 cut(s) 134, 206, 305, 323, 359, 620, 672, 887, 917
AluI AGCT 9 cut(s) 134, 206, 305, 323, 359, 620, 672, 887, 917
Alw26I GTCTC 7 cut(s) 121, 154, 203, 212, 242, 635, 728
AlwI GGATC 3 cut(s) 492, 718, 749
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 1 cut(s) 320
ApoI RAATTY 2 cut(s) 428, 702
Asp700I GAANNNNTTC 2 cut(s) 429, 836
AspLEI GCGC 1 cut(s) 950
AspS9I GGNCC 2 cut(s) 890, 896
AsuHPI GGTGA 3 cut(s) 311, 860, 886
AvaII GGWCC 2 cut(s) 890, 896
BbsI GAAGAC 1 cut(s) 792
BbvCI CCTCAGC 2 cut(s) 291, 306
BbvI GCAGC 1 cut(s) 332
BccI CCATC 1 cut(s) 248
BceAI ACGGC 1 cut(s) 7
BciT130I CCWGG 1 cut(s) 894
BcoDI GTCTC 7 cut(s) 121, 154, 203, 212, 242, 635, 728
BfaI CTAG 3 cut(s) 153, 818, 845
BisI GCNGC 2 cut(s) 157, 321
BlsI GCNGC 2 cut(s) 158, 322
BmcAI AGTACT 1 cut(s) 816
Bme1390I CCNGG 1 cut(s) 894
Bme18I GGWCC 2 cut(s) 890, 896
BmgT120I GGNCC 2 cut(s) 890, 896
BmrFI CCNGG 1 cut(s) 894
BmsI GCATC 1 cut(s) 410
BpiI GAAGAC 1 cut(s) 792
BplI GAGNNNNNCTC 2 cut(s) 123, 155
Bpu10I CCTNAGC 2 cut(s) 291, 306
BpuEI CTTGAG 1 cut(s) 552
BsaWI WCCGGW 1 cut(s) 141
BsaXI ACNNNNNCTCC 2 cut(s) 564, 594
Bsc4I CCNNNNNNNGG 1 cut(s) 634
Bse118I RCCGGY 1 cut(s) 5
Bse1I ACTGG 1 cut(s) 285
BseBI CCWGG 1 cut(s) 894
BseGI GGATG 1 cut(s) 259
BseLI CCNNNNNNNGG 1 cut(s) 634
BseMII CTCAG 6 cut(s) 198, 282, 297, 381, 525, 636
BseNI ACTGG 1 cut(s) 285
BseRI GAGGAG 1 cut(s) 308
BseXI GCAGC 1 cut(s) 332
BsgI GTGCAG 1 cut(s) 525
BshFI GGCC 1 cut(s) 5
BsiSI CCGG 3 cut(s) 6, 96, 142
BslI CCNNNNNNNGG 1 cut(s) 634
BsmAI GTCTC 7 cut(s) 121, 154, 203, 212, 242, 635, 728
BsmBI CGTCTC 4 cut(s) 121, 203, 212, 242
BsnI GGCC 1 cut(s) 5
Bsp143I GATC 3 cut(s) 484, 710, 754
BspACI CCGC 4 cut(s) 156, 169, 440, 794
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 6 cut(s) 199, 283, 298, 382, 526, 637
BspPI GGATC 3 cut(s) 492, 718, 749
BsrFI RCCGGY 1 cut(s) 5
BsrI ACTGG 1 cut(s) 285
BssAI RCCGGY 1 cut(s) 5
BssMI GATC 3 cut(s) 484, 710, 754
Bst2UI CCWGG 1 cut(s) 894
Bst4CI ACNGT 2 cut(s) 693, 739
Bst6I CTCTTC 5 cut(s) 51, 240, 521, 648, 798
BstC8I GCNNGC 2 cut(s) 438, 746
BstDEI CTNAG 6 cut(s) 207, 291, 306, 390, 534, 645
BstF5I GGATG 1 cut(s) 259
BstHHI GCGC 1 cut(s) 950
BstKTI GATC 3 cut(s) 487, 713, 757
BstMAI GTCTC 7 cut(s) 121, 154, 203, 212, 242, 635, 728
BstMBI GATC 3 cut(s) 484, 710, 754
BstMWI GCNNNNNNNGC 1 cut(s) 678
BstNI CCWGG 1 cut(s) 894
BstSCI CCNGG 1 cut(s) 892
BstV1I GCAGC 1 cut(s) 332
BstV2I GAAGAC 1 cut(s) 792
BsuRI GGCC 1 cut(s) 5
BtsCI GGATG 1 cut(s) 259
BtsIMutI CAGTG 2 cut(s) 292, 508
Cac8I GCNNGC 2 cut(s) 438, 746
CfoI GCGC 1 cut(s) 950
Cfr10I RCCGGY 1 cut(s) 5
Cfr13I GGNCC 2 cut(s) 890, 896
CseI GACGC 2 cut(s) 103, 441
Csp6I GTAC 3 cut(s) 65, 185, 815
CviAII CATG 3 cut(s) 53, 728, 767
CviQI GTAC 3 cut(s) 65, 185, 815
DdeI CTNAG 6 cut(s) 207, 291, 306, 390, 534, 645
DpnI GATC 3 cut(s) 486, 712, 756
DpnII GATC 3 cut(s) 484, 710, 754
DraI TTTAAA 1 cut(s) 408
DraIII CACNNNGTG 1 cut(s) 719
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 5 cut(s) 51, 240, 521, 648, 798
EarI CTCTTC 5 cut(s) 51, 240, 521, 648, 798
Eco47I GGWCC 2 cut(s) 890, 896
Eco57I CTGAAG 2 cut(s) 505, 939
EcoRII CCWGG 1 cut(s) 892
Esp3I CGTCTC 4 cut(s) 121, 203, 212, 242
FaeI CATG 3 cut(s) 56, 731, 770
FaiI YATR 9 cut(s) 54, 351, 416, 494, 683, 685, 729, 768, 812
FatI CATG 3 cut(s) 52, 727, 766
FblI GTMKAC 1 cut(s) 10
Fnu4HI GCNGC 2 cut(s) 157, 321
FokI GGATG 1 cut(s) 266
Fsp4HI GCNGC 2 cut(s) 157, 321
FspBI CTAG 3 cut(s) 153, 818, 845
GlaI GCGC 1 cut(s) 949
GluI GCNGC 2 cut(s) 157, 321
HaeIII GGCC 1 cut(s) 5
HapII CCGG 3 cut(s) 6, 96, 142
HgaI GACGC 2 cut(s) 103, 441
HhaI GCGC 1 cut(s) 950
Hin1II CATG 3 cut(s) 56, 731, 770
Hin6I GCGC 1 cut(s) 948
HinP1I GCGC 1 cut(s) 948
HindIII AAGCTT 1 cut(s) 357
HinfI GANTC 5 cut(s) 230, 498, 586, 788, 959
HpaII CCGG 3 cut(s) 6, 96, 142
HphI GGTGA 3 cut(s) 311, 860, 886
Hpy166II GTNNAC 1 cut(s) 11
Hpy188I TCNGA 1 cut(s) 678
Hpy188III TCNNGA 5 cut(s) 198, 271, 523, 613, 972
Hpy8I GTNNAC 1 cut(s) 11
Hpy99I CGWCG 1 cut(s) 217
HpyAV CCTTC 3 cut(s) 69, 876, 914
HpyCH4III ACNGT 2 cut(s) 693, 739
HpyCH4IV ACGT 2 cut(s) 108, 862
HpyCH4V TGCA 1 cut(s) 506
HpyF10VI GCNNNNNNNGC 1 cut(s) 678
HpyF3I CTNAG 6 cut(s) 207, 291, 306, 390, 534, 645
HpySE526I ACGT 2 cut(s) 108, 862
Hsp92II CATG 3 cut(s) 56, 731, 770
HspAI GCGC 1 cut(s) 948
Kzo9I GATC 3 cut(s) 484, 710, 754
LmnI GCTCC 1 cut(s) 61
Lsp1109I GCAGC 1 cut(s) 332
LweI GCATC 1 cut(s) 410
MaeI CTAG 3 cut(s) 153, 818, 845
MaeII ACGT 2 cut(s) 108, 862
MaeIII GTNAC 1 cut(s) 661
MalI GATC 3 cut(s) 486, 712, 756
MboI GATC 3 cut(s) 484, 710, 754
MluCI AATT 4 cut(s) 428, 607, 630, 702
MlyI GAGTC 1 cut(s) 782
MroXI GAANNNNTTC 2 cut(s) 429, 836
MseI TTAA 3 cut(s) 72, 407, 633
MspA1I CMGCKG 2 cut(s) 323, 887
MspI CCGG 3 cut(s) 6, 96, 142
MspR9I CCNGG 1 cut(s) 894
MvaI CCWGG 1 cut(s) 894
MwoI GCNNNNNNNGC 1 cut(s) 678
NdeII GATC 3 cut(s) 484, 710, 754
NlaIII CATG 3 cut(s) 56, 731, 770
NmeAIII GCCGAG 1 cut(s) 155
NmuCI GTSAC 1 cut(s) 661
PdmI GAANNNNTTC 2 cut(s) 429, 836
PfeI GAWTC 4 cut(s) 230, 498, 586, 959
PkrI GCNGC 2 cut(s) 158, 322
PleI GAGTC 1 cut(s) 782
PpsI GAGTC 1 cut(s) 782
PsiI TTATAA 1 cut(s) 494
Psp1406I AACGTT 1 cut(s) 108
Psp6I CCWGG 1 cut(s) 892
PspGI CCWGG 1 cut(s) 892
PspPI GGNCC 2 cut(s) 890, 896
PvuII CAGCTG 2 cut(s) 323, 887
RsaI GTAC 3 cut(s) 66, 186, 816
RsaNI GTAC 3 cut(s) 65, 185, 815
SaqAI TTAA 3 cut(s) 72, 407, 633
SatI GCNGC 2 cut(s) 157, 321
Sau3AI GATC 3 cut(s) 484, 710, 754
Sau96I GGNCC 2 cut(s) 890, 896
ScaI AGTACT 1 cut(s) 816
SchI GAGTC 1 cut(s) 782
ScrFI CCNGG 1 cut(s) 894
SfaNI GCATC 1 cut(s) 410
SinI GGWCC 2 cut(s) 890, 896
SmlI CTYRAG 1 cut(s) 567
SmoI CTYRAG 1 cut(s) 567
Sse9I AATT 4 cut(s) 428, 607, 630, 702
SsiI CCGC 4 cut(s) 156, 169, 440, 794
SspMI CTAG 3 cut(s) 153, 818, 845
StyD4I CCNGG 1 cut(s) 892
TaaI ACNGT 2 cut(s) 693, 739
TaiI ACGT 2 cut(s) 111, 865
TaqI TCGA 4 cut(s) 233, 270, 513, 612
TasI AATT 4 cut(s) 428, 607, 630, 702
TatI WGTACW 1 cut(s) 814
TauI GCSGC 1 cut(s) 159
TfiI GAWTC 4 cut(s) 230, 498, 586, 959
Tru1I TTAA 3 cut(s) 72, 407, 633
Tru9I TTAA 3 cut(s) 72, 407, 633
TscAI CASTG 2 cut(s) 292, 508
TseFI GTSAC 1 cut(s) 661
TseI GCWGC 1 cut(s) 320
Tsp45I GTSAC 1 cut(s) 661
TspDTI ATGAA 4 cut(s) 41, 291, 438, 957
TspGWI ACGGA 3 cut(s) 230, 236, 266
TspRI CASTG 2 cut(s) 292, 508
VpaK11BI GGWCC 2 cut(s) 890, 896
XapI RAATTY 2 cut(s) 428, 702
XmiI GTMKAC 1 cut(s) 10
XmnI GAANNNNTTC 2 cut(s) 429, 836
XspI CTAG 3 cut(s) 153, 818, 845
ZrmI AGTACT 1 cut(s) 816
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.