MD12G1014200.v1.1

Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0004571
Physical Location & Seq
Forward (+)
1473036 .. 1477246
4211 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1014200.v1.1.491

Sequence Viewer

Length: 1032 bp
ATGGCCAATGTCCTCCGAACTTCACCTTTCTCGGCGCCATTTGCAGCGCCGCCATCCGCCGCATCGCCCAACTCTAGAAAACCGTCGTACGTTTCGCTTCGGCGAAGTCAAACCAGCAGCAGCAGCACGCCTTATCTCACTATAAGCTCCCTCCGGTTCTCTCACTTGCCGTCCCTCCGGCTCGTCAAGTTCGCGCCTTTGGCTTCACAGGGTGAGGCCGAGACGGCTGGGACGGTGGAGGAGGAGGTTCAGGAGGCCGAAGAAGTCGAGGACTCTTTGGATGGTGCAATTCCTGTGGAAGATAGTACCAGTGACGGTGAAGAAAGTGGTACGAGTGATGATGGTGAAGGTGATGCTGAGGAAAAATCTGTTTCAGCCATCACAGCGTCACTCCAACAATACAAAGAAGCTTTAGCCAGTAATGATGAATCAAAAGTTGCTGAAATAGAATCTTTCCTAAAATCTTTTGAAGATGAGAAAATAGGACTTGAAATGAAAGTGGCTTCTTTGTCCGAAGAACTGTCAGCAGAGAAGGTTCGTATTCTGAGAATAAGTGCGGATTTTGACAATTTCCGGAAAAGGACAGAAAGGGAACGCATTTCTCTGGTAACAAATGCTCAGGGGGAAGTTGTGGAGAGTTTGTTGCCTGTAGTGGATAATTTTGAGAGGGCTAAAACCCAGATTAAGGTGGAGACAGAGGGAGAGGAGAAGATCAACAATAGCTATCAGAGCATTTATAAACAGTTCGGAGAGATTTTAAACTCTCTTGGTGTTGTTCCTGTTGAGACGGTGGGGAAGCCCTTTGATCCATTGTTGCATGAAGCGATTATGCGTGAGGACTCTTCAGAATACGAAGAAGGTGTTATAATCGATGAATTTCGCAAGGGGTTTAAGCTTGGTGACAGGCTTTTGCGTCCGTCAATGGTAAAGGTATCGGCTGGTCCAGGGCCTGCCAAGGTAGAGCAGGAAGTACCTCCATCTGAGGAACAGGAAGCAAGTGAAACCGCCGAGAAGGGAACCGAGTCAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GrpE

Protein Analysis

344

Amino Acids

37.4

Weight (kDa)

4.52

Isoelectric Point (pI)

55.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GrpE PF01025 162 - 312 2.4e-41 GrpE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 738, 866
AccB1I GGYRCC 1 cut(s) 34
AccII CGCG 1 cut(s) 194
AccIII TCCGGA 1 cut(s) 573
AciI CCGC 5 cut(s) 50, 57, 60, 557, 1005
AclWI GGATC 1 cut(s) 800
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 875
AcuI CTGAAG 1 cut(s) 828
AcyI GRCGYC 1 cut(s) 35
AdeI CACNNNGTG 1 cut(s) 212
AfaI GTAC 4 cut(s) 89, 307, 331, 972
AfiI CCNNNNNNNGG 1 cut(s) 685
AgsI TTSAA 2 cut(s) 470, 491
AjnI CCWGG 1 cut(s) 943
AluBI AGCT 5 cut(s) 147, 410, 723, 895, 1028
AluI AGCT 5 cut(s) 147, 410, 723, 895, 1028
Alw26I GTCTC 3 cut(s) 215, 686, 779
AlwI GGATC 1 cut(s) 800
AlwNI CAGNNNCTG 1 cut(s) 950
Aor13HI TCCGGA 1 cut(s) 573
AoxI GGCC 4 cut(s) 3, 216, 255, 947
ApeKI GCWGC 4 cut(s) 44, 117, 120, 123
ApoI RAATTY 1 cut(s) 875
AspLEI GCGC 3 cut(s) 37, 49, 196
AspS9I GGNCC 2 cut(s) 941, 947
AsuHPI GGTGA 6 cut(s) 15, 224, 329, 356, 362, 911
AvaII GGWCC 1 cut(s) 941
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 34
BbvCI CCTCAGC 1 cut(s) 357
BbvI GCAGC 4 cut(s) 56, 129, 132, 135
BccI CCATC 5 cut(s) 61, 275, 335, 386, 985
BceAI ACGGC 2 cut(s) 154, 240
BciT130I CCWGG 1 cut(s) 945
BcoDI GTCTC 3 cut(s) 215, 686, 779
BfaI CTAG 1 cut(s) 75
BfmI CTRYAG 1 cut(s) 648
BfoI RGCGCY 2 cut(s) 38, 50
BglI GCCNNNNNGGC 1 cut(s) 224
BisI GCNGC 6 cut(s) 45, 50, 60, 118, 121, 124
BlsI GCNGC 6 cut(s) 46, 51, 61, 119, 122, 125
Bme1390I CCNGG 1 cut(s) 945
Bme18I GGWCC 1 cut(s) 941
BmgT120I GGNCC 2 cut(s) 941, 947
BmiI GGNNCC 2 cut(s) 36, 1018
BmrFI CCNGG 1 cut(s) 945
BmsI GCATC 2 cut(s) 71, 343
Bpu10I CCTNAGC 2 cut(s) 357, 618
Bsa29I ATCGAT 1 cut(s) 870
BsaHI GRCGYC 1 cut(s) 35
BsaJI CCNNGG 2 cut(s) 944, 954
BsaWI WCCGGW 2 cut(s) 153, 573
BsaXI ACNNNNNCTCC 2 cut(s) 626, 656
Bsc4I CCNNNNNNNGG 1 cut(s) 685
Bse1I ACTGG 2 cut(s) 309, 417
BseAI TCCGGA 1 cut(s) 573
BseBI CCWGG 1 cut(s) 945
BseCI ATCGAT 1 cut(s) 870
BseDI CCNNGG 2 cut(s) 944, 954
BseGI GGATG 2 cut(s) 53, 286
BseLI CCNNNNNNNGG 1 cut(s) 685
BseMII CTCAG 4 cut(s) 348, 536, 632, 972
BseNI ACTGG 2 cut(s) 309, 417
BseRI GAGGAG 3 cut(s) 254, 257, 719
BseXI GCAGC 4 cut(s) 56, 129, 132, 135
BseYI CCCAGC 1 cut(s) 227
Bsh1236I CGCG 1 cut(s) 194
BshFI GGCC 4 cut(s) 5, 218, 257, 949
BshNI GGYRCC 1 cut(s) 34
BshVI ATCGAT 1 cut(s) 870
BsiSI CCGG 3 cut(s) 154, 178, 574
BsiWI CGTACG 1 cut(s) 87
BslFI GGGAC 2 cut(s) 157, 244
BslI CCNNNNNNNGG 1 cut(s) 685
BsmAI GTCTC 3 cut(s) 215, 686, 779
BsmBI CGTCTC 2 cut(s) 215, 779
BsmFI GGGAC 2 cut(s) 157, 244
BsnI GGCC 4 cut(s) 5, 218, 257, 949
Bsp13I TCCGGA 1 cut(s) 573
Bsp143I GATC 2 cut(s) 711, 805
BspACI CCGC 5 cut(s) 50, 57, 60, 557, 1005
BspANI GGCC 4 cut(s) 5, 218, 257, 949
BspCNI CTCAG 4 cut(s) 349, 537, 631, 973
BspDI ATCGAT 1 cut(s) 870
BspEI TCCGGA 1 cut(s) 573
BspFNI CGCG 1 cut(s) 194
BspLI GGNNCC 2 cut(s) 36, 1018
BspPI GGATC 1 cut(s) 800
BspT107I GGYRCC 1 cut(s) 34
BsrI ACTGG 2 cut(s) 309, 417
BssECI CCNNGG 2 cut(s) 944, 954
BssMI GATC 2 cut(s) 711, 805
BssNI GRCGYC 1 cut(s) 35
BssT1I CCWWGG 1 cut(s) 954
Bst2UI CCWGG 1 cut(s) 945
Bst4CI ACNGT 6 cut(s) 84, 235, 317, 522, 744, 790
Bst6I CTCTTC 1 cut(s) 847
BstACI GRCGYC 1 cut(s) 35
BstC8I GCNNGC 2 cut(s) 128, 951
BstDEI CTNAG 4 cut(s) 357, 545, 618, 981
BstF5I GGATG 2 cut(s) 53, 286
BstFNI CGCG 1 cut(s) 194
BstH2I RGCGCY 2 cut(s) 38, 50
BstHHI GCGC 3 cut(s) 37, 49, 196
BstKTI GATC 2 cut(s) 714, 808
BstMAI GTCTC 3 cut(s) 215, 686, 779
BstMBI GATC 2 cut(s) 711, 805
BstMWI GCNNNNNNNGC 6 cut(s) 41, 123, 200, 224, 383, 729
BstNI CCWGG 1 cut(s) 945
BstSCI CCNGG 1 cut(s) 943
BstSFI CTRYAG 1 cut(s) 648
BstUI CGCG 1 cut(s) 194
BstV1I GCAGC 4 cut(s) 56, 129, 132, 135
Bsu15I ATCGAT 1 cut(s) 870
BsuRI GGCC 4 cut(s) 5, 218, 257, 949
BsuTUI ATCGAT 1 cut(s) 870
BtgZI GCGATG 1 cut(s) 48
BtsCI GGATG 2 cut(s) 53, 286
BtsIMutI CAGTG 1 cut(s) 316
Cac8I GCNNGC 2 cut(s) 128, 951
CaiI CAGNNNCTG 1 cut(s) 950
CfoI GCGC 3 cut(s) 37, 49, 196
Cfr13I GGNCC 2 cut(s) 941, 947
ClaI ATCGAT 1 cut(s) 870
CseI GACGC 2 cut(s) 375, 902
Csp6I GTAC 4 cut(s) 88, 306, 330, 971
CspCI CAANNNNNGTGG 2 cut(s) 276, 311
CviAII CATG 1 cut(s) 818
CviQI GTAC 4 cut(s) 88, 306, 330, 971
DdeI CTNAG 4 cut(s) 357, 545, 618, 981
DinI GGCGCC 1 cut(s) 36
DpnI GATC 2 cut(s) 713, 807
DpnII GATC 2 cut(s) 711, 805
DraI TTTAAA 1 cut(s) 759
DraIII CACNNNGTG 1 cut(s) 212
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 847
EarI CTCTTC 1 cut(s) 847
EciI GGCGGA 1 cut(s) 46
Eco130I CCWWGG 1 cut(s) 954
Eco47I GGWCC 1 cut(s) 941
Eco57I CTGAAG 1 cut(s) 828
EcoO109I RGGNCCY 1 cut(s) 947
EcoRII CCWGG 1 cut(s) 943
EcoT14I CCWWGG 1 cut(s) 954
EgeI GGCGCC 1 cut(s) 36
EheI GGCGCC 1 cut(s) 36
ErhI CCWWGG 1 cut(s) 954
Esp3I CGTCTC 2 cut(s) 215, 779
FaeI CATG 1 cut(s) 821
FaiI YATR 5 cut(s) 143, 738, 819, 830, 866
FaqI GGGAC 2 cut(s) 157, 244
FatI CATG 1 cut(s) 817
Fnu4HI GCNGC 6 cut(s) 45, 50, 60, 118, 121, 124
FokI GGATG 2 cut(s) 40, 293
Fsp4HI GCNGC 6 cut(s) 45, 50, 60, 118, 121, 124
FspBI CTAG 1 cut(s) 75
GlaI GCGC 3 cut(s) 36, 48, 195
GluI GCNGC 6 cut(s) 45, 50, 60, 118, 121, 124
GsaI CCCAGC 1 cut(s) 231
HaeII RGCGCY 2 cut(s) 38, 50
HaeIII GGCC 4 cut(s) 5, 218, 257, 949
HapII CCGG 3 cut(s) 154, 178, 574
HgaI GACGC 2 cut(s) 375, 902
HhaI GCGC 3 cut(s) 37, 49, 196
Hin1I GRCGYC 1 cut(s) 35
Hin1II CATG 1 cut(s) 821
Hin6I GCGC 3 cut(s) 35, 47, 194
HinP1I GCGC 3 cut(s) 35, 47, 194
HindIII AAGCTT 2 cut(s) 408, 893
HinfI GANTC 5 cut(s) 272, 428, 449, 839, 1022
HpaII CCGG 3 cut(s) 154, 178, 574
HphI GGTGA 6 cut(s) 15, 224, 329, 356, 362, 911
Hpy188I TCNGA 7 cut(s) 17, 514, 546, 729, 749, 847, 982
Hpy188III TCNNGA 3 cut(s) 75, 251, 574
Hpy99I CGWCG 1 cut(s) 88
HpyAV CCTTC 4 cut(s) 341, 526, 851, 1006
HpyCH4III ACNGT 6 cut(s) 84, 235, 317, 522, 744, 790
HpyCH4IV ACGT 1 cut(s) 90
HpyCH4V TGCA 3 cut(s) 44, 287, 817
HpyF10VI GCNNNNNNNGC 6 cut(s) 41, 123, 200, 224, 383, 729
HpyF3I CTNAG 4 cut(s) 357, 545, 618, 981
HpySE526I ACGT 1 cut(s) 90
Hsp92I GRCGYC 1 cut(s) 35
Hsp92II CATG 1 cut(s) 821
HspAI GCGC 3 cut(s) 35, 47, 194
KasI GGCGCC 1 cut(s) 34
Kpn2I TCCGGA 1 cut(s) 573
Kzo9I GATC 2 cut(s) 711, 805
LmnI GCTCC 1 cut(s) 152
Lsp1109I GCAGC 4 cut(s) 56, 129, 132, 135
LweI GCATC 2 cut(s) 71, 343
MaeI CTAG 1 cut(s) 75
MaeII ACGT 1 cut(s) 90
MaeIII GTNAC 4 cut(s) 311, 387, 607, 899
MalI GATC 2 cut(s) 713, 807
MboI GATC 2 cut(s) 711, 805
MboII GAAGA 8 cut(s) 272, 311, 332, 482, 527, 721, 834, 866
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 4 cut(s) 288, 568, 658, 875
MluNI TGGCCA 1 cut(s) 5
Mly113I GGCGCC 1 cut(s) 35
MlyI GAGTC 3 cut(s) 266, 833, 1031
MmeI TCCRAC 1 cut(s) 418
Mox20I TGGCCA 1 cut(s) 5
MroI TCCGGA 1 cut(s) 573
MscI TGGCCA 1 cut(s) 5
MseI TTAA 4 cut(s) 684, 758, 891, 1030
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 3 cut(s) 154, 178, 574
MspR9I CCNGG 1 cut(s) 945
MvaI CCWGG 1 cut(s) 945
MvnI CGCG 1 cut(s) 194
MwoI GCNNNNNNNGC 6 cut(s) 41, 123, 200, 224, 383, 729
NarI GGCGCC 1 cut(s) 35
NdeII GATC 2 cut(s) 711, 805
NlaIII CATG 1 cut(s) 821
NlaIV GGNNCC 2 cut(s) 36, 1018
NmeAIII GCCGAG 2 cut(s) 11, 244
NmuCI GTSAC 3 cut(s) 311, 387, 899
PfeI GAWTC 2 cut(s) 428, 449
Pfl23II CGTACG 1 cut(s) 87
PkrI GCNGC 6 cut(s) 46, 51, 61, 119, 122, 125
PleI GAGTC 3 cut(s) 266, 833, 1030
PluTI GGCGCC 1 cut(s) 38
PpsI GAGTC 3 cut(s) 266, 833, 1030
PsiI TTATAA 2 cut(s) 738, 866
Psp6I CCWGG 1 cut(s) 943
PspFI CCCAGC 1 cut(s) 227
PspGI CCWGG 1 cut(s) 943
PspLI CGTACG 1 cut(s) 87
PspN4I GGNNCC 2 cut(s) 36, 1018
PspPI GGNCC 2 cut(s) 941, 947
PstNI CAGNNNCTG 1 cut(s) 950
RsaI GTAC 4 cut(s) 89, 307, 331, 972
RsaNI GTAC 4 cut(s) 88, 306, 330, 971
SaqAI TTAA 4 cut(s) 684, 758, 891, 1030
SatI GCNGC 6 cut(s) 45, 50, 60, 118, 121, 124
Sau3AI GATC 2 cut(s) 711, 805
Sau96I GGNCC 2 cut(s) 941, 947
SchI GAGTC 3 cut(s) 266, 833, 1031
ScrFI CCNGG 1 cut(s) 945
SfaNI GCATC 2 cut(s) 71, 343
SfcI CTRYAG 1 cut(s) 648
SfoI GGCGCC 1 cut(s) 36
SinI GGWCC 1 cut(s) 941
Sse9I AATT 4 cut(s) 288, 568, 658, 875
SsiI CCGC 5 cut(s) 50, 57, 60, 557, 1005
SspDI GGCGCC 1 cut(s) 34
SspMI CTAG 1 cut(s) 75
StyD4I CCNGG 1 cut(s) 943
StyI CCWWGG 1 cut(s) 954
TaaI ACNGT 6 cut(s) 84, 235, 317, 522, 744, 790
TaiI ACGT 1 cut(s) 93
TaqI TCGA 2 cut(s) 267, 870
TasI AATT 4 cut(s) 288, 568, 658, 875
TauI GCSGC 2 cut(s) 52, 62
TfiI GAWTC 2 cut(s) 428, 449
Tru1I TTAA 4 cut(s) 684, 758, 891, 1030
Tru9I TTAA 4 cut(s) 684, 758, 891, 1030
TscAI CASTG 1 cut(s) 316
TseFI GTSAC 3 cut(s) 311, 387, 899
TseI GCWGC 4 cut(s) 44, 117, 120, 123
Tsp45I GTSAC 3 cut(s) 311, 387, 899
TspDTI ATGAA 4 cut(s) 441, 509, 834, 888
TspGWI ACGGA 1 cut(s) 906
TspRI CASTG 1 cut(s) 316
VpaK11BI GGWCC 1 cut(s) 941
XapI RAATTY 1 cut(s) 875
XbaI TCTAGA 1 cut(s) 74
XspI CTAG 1 cut(s) 75
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.