Rorug03G0275100

Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0004571
Physical Location & Seq
Reverse (-)
27169787 .. 27175208
5422 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0275100.1

Sequence Viewer

Length: 1260 bp
ATGGCAGCTCGGAAGCGAGCTTCAACAAACTCGTCCTCCTCGACTCAGACCAAACCAAGCGATCGCGAGCCACCCAAAACCCAAGAAGCGCCAATCTCAGCACCCAAGTCTGGTGTGATTCTCAAGCTGACGGCGTTGTTCTCCGTCCCCTACTTTTACCTGGTCTTGTACCATTACAAGATTGAGCAGGAGCTCAGAAATTCGATTCTGATCAATGCCGCCCTCAGCCTGGCGGCGTTCTTCGTCACCGTTAAGTTGATCCCTGTGGCTTCTAGATACGTTCTCAGGCGCAATCTGTTTGGCTACGATATTAACAAAAAGGGTACTCCTCAGGGTACTATTAAAGTGCCAGAGTCGTTGGGCATTGTTGTTGGAATTGTCTTCTTGGTCTTTTCGATATTATTTCAGTATTTCAACTTCACTGCAGATTCAAATTGGCTTGTTGAGTACAATGCAGCATTAGCCTCCATATGCCTTATGGTATTGCTGGGATTTGTAGATGATGTTCTTGATGTACCGTGGAGAGTGAAATTAATACTGCCATCAATTGCAGCTTTACCTCTGCTGATGGCTTATGCTGGACATACAACCATTATCATACCGAAGCCTCTTGTTCCATATGTTGGGCTAGAGGTCTGGGATCTAGGATGGATGTATAAACTATATATGGGACTTTTGGCGGTTTTCTGTACAAACTCGATTAATATTCATGCTGGCTTGAATGGTCTTGAAGTTGGCCAGACAGTTGTAATTGCATCTGCTATTTTAATACACAATATCATGCAAATTGGAGCATCTGTAGATTTTGAGTACAAGATGGCACACGCATTCTCTATATATCTTGCCCAACCCTTACTGGCAACCTCCTTGGCATTGTTCTCTTACAACTGGTATCCTTCTTCAGCTTTTGTTGGAGATACTTACACATACTTTGCTGGGATGACTATGGCTGTAGTTGGGATTCTTGGCCATTTTAGTGAAACGCTCCTGATTTTCTTTCTCCCTCAAGTCTTGAACTTTCTCTTGTCGCTGCCCCAGCTTGCTGGGATCATTCCATGTCCGCGCCATCGTCTCCCAAGGTTCGATCCTGAAACTGGACTGCTGACTGGGACAAATGATGGGACGCTTGTAAACTTGTTCCTAAGACAATTTGGCAGGATGACAGAGAAGAGACTTTGTGTTTATCTTCTCGTTTTCCAGGCTCTAGCATGCTGCTTCTGTTTCCTGTTGAGATACTTCCTTGCTGGTTGGTATAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GrpE

Protein Analysis

419

Amino Acids

46.71

Weight (kDa)

9.01

Isoelectric Point (pI)

37.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glycos_transf_4 PF00953 153 - 323 5.4e-27 Glycosyl transferase family 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 623
AccII CGCG 2 cut(s) 66, 1063
AciI CCGC 4 cut(s) 219, 233, 680, 1061
AclWI GGATC 4 cut(s) 253, 648, 1055, 1079
AcoI YGGCCR 2 cut(s) 736, 967
AcsI RAATTY 1 cut(s) 199
AcuI CTGAAG 1 cut(s) 885
AfaI GTAC 7 cut(s) 170, 325, 337, 449, 516, 691, 812
AfiI CCNNNNNNNGG 4 cut(s) 110, 229, 623, 1094
AgsI TTSAA 6 cut(s) 24, 415, 432, 721, 731, 1015
AjnI CCWGG 3 cut(s) 159, 228, 1197
AluBI AGCT 7 cut(s) 8, 20, 127, 193, 554, 905, 1039
AluI AGCT 7 cut(s) 8, 20, 127, 193, 554, 905, 1039
Alw21I GWGCWC 1 cut(s) 195
Alw26I GTCTC 2 cut(s) 1076, 1165
AlwI GGATC 4 cut(s) 253, 648, 1055, 1079
AoxI GGCC 2 cut(s) 736, 967
ApeKI GCWGC 5 cut(s) 5, 455, 551, 1030, 1212
ApoI RAATTY 1 cut(s) 199
AseI ATTAAT 2 cut(s) 533, 702
AsiSI GCGATCGC 1 cut(s) 64
AspLEI GCGC 3 cut(s) 91, 291, 1065
AsuHPI GGTGA 1 cut(s) 238
AxyI CCTNAGG 1 cut(s) 330
BalI TGGCCA 2 cut(s) 738, 969
BanII GRGCYC 1 cut(s) 195
BarI GAAGNNNNNNTAC 2 cut(s) 401, 433
BbsI GAAGAC 1 cut(s) 373
Bbv12I GWGCWC 1 cut(s) 195
BbvCI CCTCAGC 1 cut(s) 224
BbvI GCAGC 5 cut(s) 17, 467, 563, 1017, 1199
BccI CCATC 6 cut(s) 550, 562, 642, 811, 1074, 1112
BceAI ACGGC 1 cut(s) 147
BciT130I CCWGG 3 cut(s) 161, 230, 1199
BciVI GTATCC 1 cut(s) 903
BclI TGATCA 1 cut(s) 210
BcoDI GTCTC 2 cut(s) 1076, 1165
BfaI CTAG 4 cut(s) 273, 629, 644, 1205
BfmI CTRYAG 3 cut(s) 423, 798, 951
BfoI RGCGCY 1 cut(s) 92
BfuI GTATCC 1 cut(s) 903
BisI GCNGC 7 cut(s) 6, 219, 234, 456, 552, 1031, 1213
BlsI GCNGC 7 cut(s) 7, 220, 235, 457, 553, 1032, 1214
Bme1390I CCNGG 3 cut(s) 161, 230, 1199
BmrFI CCNGG 3 cut(s) 161, 230, 1199
BmrI ACTGGG 1 cut(s) 1116
BmsI GCATC 2 cut(s) 764, 803
BmuI ACTGGG 1 cut(s) 1116
BpiI GAAGAC 1 cut(s) 373
Bpu10I CCTNAGC 1 cut(s) 224
BpuEI CTTGAG 2 cut(s) 107, 990
BsaBI GATNNNNATC 1 cut(s) 209
BsaJI CCNNGG 3 cut(s) 518, 867, 1076
BsaXI ACNNNNNCTCC 4 cut(s) 20, 50, 783, 813
Bsc4I CCNNNNNNNGG 4 cut(s) 110, 229, 623, 1094
Bse1I ACTGG 4 cut(s) 861, 893, 1099, 1111
Bse21I CCTNAGG 1 cut(s) 330
Bse8I GATNNNNATC 1 cut(s) 209
BseBI CCWGG 3 cut(s) 161, 230, 1199
BseDI CCNNGG 3 cut(s) 518, 867, 1076
BseGI GGATG 4 cut(s) 653, 657, 945, 1164
BseJI GATNNNNATC 1 cut(s) 209
BseLI CCNNNNNNNGG 4 cut(s) 110, 229, 623, 1094
BseMII CTCAG 6 cut(s) 59, 111, 208, 238, 298, 344
BseNI ACTGG 4 cut(s) 861, 893, 1099, 1111
BseRI GAGGAG 2 cut(s) 28, 318
BseXI GCAGC 5 cut(s) 17, 467, 563, 1017, 1199
BseYI CCCAGC 4 cut(s) 487, 935, 1035, 1043
Bsh1236I CGCG 2 cut(s) 66, 1063
Bsh1285I CGRYCG 1 cut(s) 64
BshFI GGCC 2 cut(s) 738, 969
BsiEI CGRYCG 1 cut(s) 64
BsiHKAI GWGCWC 1 cut(s) 195
BslFI GGGAC 4 cut(s) 131, 684, 1123, 1135
BslI CCNNNNNNNGG 4 cut(s) 110, 229, 623, 1094
BsmAI GTCTC 2 cut(s) 1076, 1165
BsmBI CGTCTC 1 cut(s) 1076
BsmFI GGGAC 4 cut(s) 131, 684, 1123, 1135
BsmI GAATGC 1 cut(s) 827
BsnI GGCC 2 cut(s) 738, 969
Bsp1286I GDGCHC 1 cut(s) 195
Bsp1407I TGTACA 1 cut(s) 689
Bsp143I GATC 6 cut(s) 61, 210, 258, 640, 1047, 1084
Bsp68I TCGCGA 1 cut(s) 66
BspACI CCGC 4 cut(s) 219, 233, 680, 1061
BspANI GGCC 2 cut(s) 738, 969
BspCNI CTCAG 6 cut(s) 58, 110, 207, 237, 297, 343
BspFNI CGCG 2 cut(s) 66, 1063
BspMAI CTGCAG 1 cut(s) 427
BspPI GGATC 4 cut(s) 253, 648, 1055, 1079
BsrGI TGTACA 1 cut(s) 689
BsrI ACTGG 4 cut(s) 861, 893, 1099, 1111
BssECI CCNNGG 3 cut(s) 518, 867, 1076
BssMI GATC 6 cut(s) 61, 210, 258, 640, 1047, 1084
BssT1I CCWWGG 2 cut(s) 867, 1076
Bst2UI CCWGG 3 cut(s) 161, 230, 1199
Bst4CI ACNGT 3 cut(s) 250, 519, 745
Bst6I CTCTTC 1 cut(s) 1163
BstAUI TGTACA 1 cut(s) 689
BstC8I GCNNGC 5 cut(s) 18, 68, 715, 1041, 1210
BstDEI CTNAG 7 cut(s) 45, 97, 194, 224, 284, 330, 1142
BstDSI CCRYGG 1 cut(s) 518
BstF5I GGATG 4 cut(s) 653, 657, 945, 1164
BstFNI CGCG 2 cut(s) 66, 1063
BstH2I RGCGCY 1 cut(s) 92
BstHHI GCGC 3 cut(s) 91, 291, 1065
BstKTI GATC 6 cut(s) 64, 213, 261, 643, 1050, 1087
BstMAI GTCTC 2 cut(s) 1076, 1165
BstMBI GATC 6 cut(s) 61, 210, 258, 640, 1047, 1084
BstMCI CGRYCG 1 cut(s) 64
BstMWI GCNNNNNNNGC 2 cut(s) 461, 1036
BstNI CCWGG 3 cut(s) 161, 230, 1199
BstNSI RCATGY 1 cut(s) 1212
BstSCI CCNGG 3 cut(s) 159, 228, 1197
BstSFI CTRYAG 3 cut(s) 423, 798, 951
BstUI CGCG 2 cut(s) 66, 1063
BstV1I GCAGC 5 cut(s) 17, 467, 563, 1017, 1199
BstV2I GAAGAC 1 cut(s) 373
BstX2I RGATCY 1 cut(s) 640
BstXI CCANNNNNNTGG 1 cut(s) 1043
BstYI RGATCY 1 cut(s) 640
Bsu36I CCTNAGG 1 cut(s) 330
BsuI GTATCC 1 cut(s) 903
BsuRI GGCC 2 cut(s) 738, 969
BtgI CCRYGG 1 cut(s) 518
BtsCI GGATG 4 cut(s) 653, 657, 945, 1164
BtsI GCAGTG 1 cut(s) 420
BtsIMutI CAGTG 1 cut(s) 420
BtuMI TCGCGA 1 cut(s) 66
Cac8I GCNNGC 5 cut(s) 18, 68, 715, 1041, 1210
CfoI GCGC 3 cut(s) 91, 291, 1065
CseI GACGC 1 cut(s) 1132
CsiI ACCWGGT 1 cut(s) 159
Csp6I GTAC 7 cut(s) 169, 324, 336, 448, 515, 690, 811
CviAII CATG 4 cut(s) 710, 781, 1056, 1209
CviQI GTAC 7 cut(s) 169, 324, 336, 448, 515, 690, 811
DdeI CTNAG 7 cut(s) 45, 97, 194, 224, 284, 330, 1142
DpnI GATC 6 cut(s) 63, 212, 260, 642, 1049, 1086
DpnII GATC 6 cut(s) 61, 210, 258, 640, 1047, 1084
EaeI YGGCCR 2 cut(s) 736, 967
Eam1104I CTCTTC 1 cut(s) 1163
EarI CTCTTC 1 cut(s) 1163
Ecl136II GAGCTC 1 cut(s) 193
Eco130I CCWWGG 2 cut(s) 867, 1076
Eco24I GRGCYC 1 cut(s) 195
Eco53kI GAGCTC 1 cut(s) 193
Eco57I CTGAAG 1 cut(s) 885
Eco81I CCTNAGG 1 cut(s) 330
EcoICRI GAGCTC 1 cut(s) 193
EcoRII CCWGG 3 cut(s) 159, 228, 1197
EcoT14I CCWWGG 2 cut(s) 867, 1076
EcoT38I GRGCYC 1 cut(s) 195
ErhI CCWWGG 2 cut(s) 867, 1076
Esp3I CGTCTC 1 cut(s) 1076
FaeI CATG 4 cut(s) 713, 784, 1059, 1212
FaqI GGGAC 4 cut(s) 131, 684, 1123, 1135
FatI CATG 4 cut(s) 709, 780, 1055, 1208
FauNDI CATATG 2 cut(s) 470, 619
FbaI TGATCA 1 cut(s) 210
Fnu4HI GCNGC 7 cut(s) 6, 219, 234, 456, 552, 1031, 1213
FokI GGATG 4 cut(s) 660, 664, 952, 1171
FriOI GRGCYC 1 cut(s) 195
Fsp4HI GCNGC 7 cut(s) 6, 219, 234, 456, 552, 1031, 1213
FspBI CTAG 4 cut(s) 273, 629, 644, 1205
GlaI GCGC 3 cut(s) 90, 290, 1064
GluI GCNGC 7 cut(s) 6, 219, 234, 456, 552, 1031, 1213
GsaI CCCAGC 4 cut(s) 491, 939, 1039, 1047
HaeII RGCGCY 1 cut(s) 92
HaeIII GGCC 2 cut(s) 738, 969
HgaI GACGC 1 cut(s) 1132
HhaI GCGC 3 cut(s) 91, 291, 1065
Hin1II CATG 4 cut(s) 713, 784, 1059, 1212
Hin6I GCGC 3 cut(s) 89, 289, 1063
HinP1I GCGC 3 cut(s) 89, 289, 1063
HinfI GANTC 6 cut(s) 43, 118, 205, 353, 428, 961
HphI GGTGA 1 cut(s) 238
Hpy166II GTNNAC 1 cut(s) 1132
Hpy188I TCNGA 4 cut(s) 12, 48, 197, 210
Hpy188III TCNNGA 7 cut(s) 65, 273, 509, 728, 988, 1012, 1088
Hpy8I GTNNAC 1 cut(s) 1132
HpyAV CCTTC 1 cut(s) 906
HpyCH4III ACNGT 3 cut(s) 250, 519, 745
HpyCH4IV ACGT 1 cut(s) 279
HpyCH4V TGCA 5 cut(s) 425, 455, 551, 755, 784
HpyF10VI GCNNNNNNNGC 2 cut(s) 461, 1036
HpyF3I CTNAG 7 cut(s) 45, 97, 194, 224, 284, 330, 1142
HpySE526I ACGT 1 cut(s) 279
Hsp92II CATG 4 cut(s) 713, 784, 1059, 1212
HspAI GCGC 3 cut(s) 89, 289, 1063
Ksp22I TGATCA 1 cut(s) 210
Kzo9I GATC 6 cut(s) 61, 210, 258, 640, 1047, 1084
LmnI GCTCC 3 cut(s) 190, 791, 990
Lsp1109I GCAGC 5 cut(s) 17, 467, 563, 1017, 1199
LweI GCATC 2 cut(s) 764, 803
MabI ACCWGGT 1 cut(s) 159
MaeI CTAG 4 cut(s) 273, 629, 644, 1205
MaeII ACGT 1 cut(s) 279
MaeIII GTNAC 1 cut(s) 244
MalI GATC 6 cut(s) 63, 212, 260, 642, 1049, 1086
MboI GATC 6 cut(s) 61, 210, 258, 640, 1047, 1084
MboII GAAGA 5 cut(s) 232, 373, 891, 1178, 1180
MfeI CAATTG 1 cut(s) 546
MflI RGATCY 1 cut(s) 640
MhlI GDGCHC 1 cut(s) 195
MlsI TGGCCA 2 cut(s) 738, 969
MluCI AATT 8 cut(s) 199, 375, 433, 530, 546, 750, 786, 1148
MluNI TGGCCA 2 cut(s) 738, 969
MlyI GAGTC 2 cut(s) 37, 362
MmeI TCCRAC 2 cut(s) 352, 892
Mox20I TGGCCA 2 cut(s) 738, 969
MscI TGGCCA 2 cut(s) 738, 969
MseI TTAA 6 cut(s) 252, 312, 342, 533, 702, 767
MslI CAYNNNNRTG 1 cut(s) 975
Msp20I TGGCCA 2 cut(s) 738, 969
MspR9I CCNGG 3 cut(s) 161, 230, 1199
MunI CAATTG 1 cut(s) 546
Mva1269I GAATGC 1 cut(s) 827
MvaI CCWGG 3 cut(s) 161, 230, 1199
MvnI CGCG 2 cut(s) 66, 1063
MwoI GCNNNNNNNGC 2 cut(s) 461, 1036
NdeI CATATG 2 cut(s) 470, 619
NdeII GATC 6 cut(s) 61, 210, 258, 640, 1047, 1084
NlaIII CATG 4 cut(s) 713, 784, 1059, 1212
NmuCI GTSAC 1 cut(s) 244
NruI TCGCGA 1 cut(s) 66
NspI RCATGY 1 cut(s) 1212
PaeI GCATGC 1 cut(s) 1212
PcsI WCGNNNNNNNCGW 1 cut(s) 38
PctI GAATGC 1 cut(s) 827
PfeI GAWTC 4 cut(s) 118, 205, 428, 961
PflMI CCANNNNNTGG 1 cut(s) 623
PkrI GCNGC 7 cut(s) 7, 220, 235, 457, 553, 1032, 1214
Ple19I CGATCG 1 cut(s) 64
PleI GAGTC 2 cut(s) 37, 361
PpsI GAGTC 2 cut(s) 37, 361
PshBI ATTAAT 2 cut(s) 533, 702
Psp124BI GAGCTC 1 cut(s) 195
Psp6I CCWGG 3 cut(s) 159, 228, 1197
PspFI CCCAGC 4 cut(s) 487, 935, 1035, 1043
PspGI CCWGG 3 cut(s) 159, 228, 1197
PsrI GAACNNNNNNTAC 2 cut(s) 489, 521
PstI CTGCAG 1 cut(s) 427
PsuI RGATCY 1 cut(s) 640
PvuI CGATCG 1 cut(s) 64
RgaI GCGATCGC 1 cut(s) 64
RruI TCGCGA 1 cut(s) 66
RsaI GTAC 7 cut(s) 170, 325, 337, 449, 516, 691, 812
RsaNI GTAC 7 cut(s) 169, 324, 336, 448, 515, 690, 811
RseI CAYNNNNRTG 1 cut(s) 975
SacI GAGCTC 1 cut(s) 195
SaqAI TTAA 6 cut(s) 252, 312, 342, 533, 702, 767
SatI GCNGC 7 cut(s) 6, 219, 234, 456, 552, 1031, 1213
Sau3AI GATC 6 cut(s) 61, 210, 258, 640, 1047, 1084
SchI GAGTC 2 cut(s) 37, 362
ScrFI CCNGG 3 cut(s) 161, 230, 1199
SduI GDGCHC 1 cut(s) 195
SexAI ACCWGGT 1 cut(s) 159
SfaAI GCGATCGC 1 cut(s) 64
SfaNI GCATC 2 cut(s) 764, 803
SfcI CTRYAG 3 cut(s) 423, 798, 951
SgfI GCGATCGC 1 cut(s) 64
SmiMI CAYNNNNRTG 1 cut(s) 975
SmlI CTYRAG 2 cut(s) 122, 1005
SmoI CTYRAG 2 cut(s) 122, 1005
SphI GCATGC 1 cut(s) 1212
Sse9I AATT 8 cut(s) 199, 375, 433, 530, 546, 750, 786, 1148
SsiI CCGC 4 cut(s) 219, 233, 680, 1061
SspI AATATT 1 cut(s) 706
SspMI CTAG 4 cut(s) 273, 629, 644, 1205
SstI GAGCTC 1 cut(s) 195
StyD4I CCNGG 3 cut(s) 159, 228, 1197
StyI CCWWGG 2 cut(s) 867, 1076
TaaI ACNGT 3 cut(s) 250, 519, 745
TaiI ACGT 1 cut(s) 282
TaqI TCGA 5 cut(s) 41, 203, 395, 698, 1083
TasI AATT 8 cut(s) 199, 375, 433, 530, 546, 750, 786, 1148
TatI WGTACW 3 cut(s) 447, 689, 810
TauI GCSGC 2 cut(s) 221, 236
TfiI GAWTC 4 cut(s) 118, 205, 428, 961
Tru1I TTAA 6 cut(s) 252, 312, 342, 533, 702, 767
Tru9I TTAA 6 cut(s) 252, 312, 342, 533, 702, 767
TscAI CASTG 1 cut(s) 427
TseFI GTSAC 1 cut(s) 244
TseI GCWGC 5 cut(s) 5, 455, 551, 1030, 1212
Tsp45I GTSAC 1 cut(s) 244
TspDTI ATGAA 1 cut(s) 698
TspGWI ACGGA 1 cut(s) 133
TspRI CASTG 1 cut(s) 427
Van91I CCANNNNNTGG 1 cut(s) 623
VspI ATTAAT 2 cut(s) 533, 702
XapI RAATTY 1 cut(s) 199
XbaI TCTAGA 1 cut(s) 272
XceI RCATGY 1 cut(s) 1212
XcmI CCANNNNNNNNNTGG 1 cut(s) 475
XspI CTAG 4 cut(s) 273, 629, 644, 1205
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.