Rh3AG322500

Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0004571
Physical Location & Seq
Reverse (-)
45139317 .. 45144257
4941 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG322500.1

Sequence Viewer

Length: 915 bp
ATGCAATTTTTGTTCCACAGAGAAAAGACGAAGACGGTATTTTTTATTGTTATTGTTGTTATTGTTATTGTTTGTTTGTTTCTTCTTTCCTTTAGGGTTTATTTTTTTACTTTACTTCTAGGTACTGATGCTAATAGATATATGCAGGATTCCTTAGACGGGGCAGTTAGTGTGGAAGATAGTACAAGTGATGCTGAAGAAAGTGGTACAAGTGATGATGGGGAAGGTGATGACAAAAAACCTGTTTCAGCCATCATAGCGTCACTACAATTATACAAAGAAGCTTTAGCCAGTAATGATGAATCAAAAGTAGCCGAGATTGAATCTTTTCTAAAATCTGTTGAAGATGAAAAAATAGACCTTGAAAGGAAAGTGGCTTCGTTGTCTGAAGAACTGTCAGCAGGGAAGGTTCGGATTCTGAGGATTAGTGCAGACTTTGATAATTTCCGGAAAAGGACAGATAGAGAACGCATTTCACTGGTAACTAATGCTCAGGGGGAAGTTGTGGAGAGCTTGTTGGGTGTATTGGATAATTTTGAGAGGGCTAAAGCCCAGATTAAGGTGGAGACAGAGGGAGAGGAGAAGATCAACAATAGCTATCAGAGCATTTATAAACAGTTTGGAGAAATCTTAAGTTCACTTGGCGTTCTCCCGGTGGAGACAGTGGGGAAGCCCTTTGATCCGTTGTTGCATGAAGCAATTATGCGTGAGGAGTCTTCAGAATTTGAAGAAGGTATTATACTAGATGAATTTCGCAAGGGGTTTAAGCTTGGTGACAGGCTTCTGCGTCCATCAATGGTAAAGGTATCAGCTGGTCCAGGGCCTGCCAAGCCAGAGCAGCAAGTAGCTCCATCTGAGGAACATGATGCAAGTGAAGCGACCAAGGAGGACATCACAGAAACTGAGTCGGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GrpE

Protein Analysis

304

Amino Acids

33.86

Weight (kDa)

4.61

Isoelectric Point (pI)

36.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GrpE PF01025 114 - 270 3.7e-40 GrpE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 612
AccIII TCCGGA 1 cut(s) 447
AclWI GGATC 1 cut(s) 674
AcsI RAATTY 2 cut(s) 722, 749
AcuI CTGAAG 3 cut(s) 216, 408, 702
AfaI GTAC 3 cut(s) 124, 184, 208
AfiI CCNNNNNNNGG 2 cut(s) 159, 559
AflII CTTAAG 1 cut(s) 631
AgsI TTSAA 4 cut(s) 323, 344, 365, 728
AjnI CCWGG 1 cut(s) 817
AluBI AGCT 6 cut(s) 284, 513, 597, 769, 812, 848
AluI AGCT 6 cut(s) 284, 513, 597, 769, 812, 848
Alw26I GTCTC 2 cut(s) 560, 653
AlwI GGATC 1 cut(s) 674
AlwNI CAGNNNCTG 2 cut(s) 824, 902
Aor13HI TCCGGA 1 cut(s) 447
AoxI GGCC 1 cut(s) 821
ApeKI GCWGC 1 cut(s) 838
ApoI RAATTY 2 cut(s) 722, 749
Asp700I GAANNNNTTC 1 cut(s) 327
AspS9I GGNCC 2 cut(s) 815, 821
AsuC2I CCSGG 1 cut(s) 653
AsuHPI GGTGA 2 cut(s) 239, 785
AvaII GGWCC 1 cut(s) 815
BarI GAAGNNNNNNTAC 2 cut(s) 723, 755
BbsI GAAGAC 2 cut(s) 38, 708
BbvI GCAGC 1 cut(s) 850
BccI CCATC 4 cut(s) 212, 260, 799, 859
BciT130I CCWGG 1 cut(s) 819
BcnI CCSGG 1 cut(s) 653
BcoDI GTCTC 2 cut(s) 560, 653
BfaI CTAG 2 cut(s) 119, 743
BfrI CTTAAG 1 cut(s) 631
BisI GCNGC 1 cut(s) 839
BlsI GCNGC 1 cut(s) 840
Bme1390I CCNGG 2 cut(s) 653, 819
Bme18I GGWCC 1 cut(s) 815
BmgT120I GGNCC 2 cut(s) 815, 821
BmrFI CCNGG 2 cut(s) 653, 819
BmsI GCATC 3 cut(s) 118, 181, 856
BpiI GAAGAC 2 cut(s) 38, 708
Bpu10I CCTNAGC 1 cut(s) 492
BpuMI CCSGG 1 cut(s) 653
BsaJI CCNNGG 2 cut(s) 818, 882
BsaWI WCCGGW 1 cut(s) 447
BsaXI ACNNNNNCTCC 2 cut(s) 500, 530
Bsc4I CCNNNNNNNGG 2 cut(s) 159, 559
Bse1I ACTGG 2 cut(s) 291, 483
BseAI TCCGGA 1 cut(s) 447
BseBI CCWGG 1 cut(s) 819
BseDI CCNNGG 2 cut(s) 818, 882
BseLI CCNNNNNNNGG 2 cut(s) 159, 559
BseMII CTCAG 4 cut(s) 410, 506, 846, 894
BseNI ACTGG 2 cut(s) 291, 483
BseRI GAGGAG 2 cut(s) 593, 725
BseXI GCAGC 1 cut(s) 850
BsgI GTGCAG 1 cut(s) 450
BshFI GGCC 1 cut(s) 823
BsiSI CCGG 2 cut(s) 448, 653
BslI CCNNNNNNNGG 2 cut(s) 159, 559
BsmAI GTCTC 2 cut(s) 560, 653
BsnI GGCC 1 cut(s) 823
Bsp13I TCCGGA 1 cut(s) 447
Bsp143I GATC 2 cut(s) 585, 679
BspANI GGCC 1 cut(s) 823
BspCNI CTCAG 4 cut(s) 411, 505, 847, 895
BspEI TCCGGA 1 cut(s) 447
BspPI GGATC 1 cut(s) 674
BspTI CTTAAG 1 cut(s) 631
BsrI ACTGG 2 cut(s) 291, 483
BssECI CCNNGG 2 cut(s) 818, 882
BssMI GATC 2 cut(s) 585, 679
BssT1I CCWWGG 1 cut(s) 882
Bst2UI CCWGG 1 cut(s) 819
Bst4CI ACNGT 4 cut(s) 37, 396, 618, 664
BstAFI CTTAAG 1 cut(s) 631
BstC8I GCNNGC 1 cut(s) 825
BstDEI CTNAG 5 cut(s) 154, 419, 492, 855, 903
BstKTI GATC 2 cut(s) 588, 682
BstMAI GTCTC 2 cut(s) 560, 653
BstMBI GATC 2 cut(s) 585, 679
BstMWI GCNNNNNNNGC 5 cut(s) 257, 603, 829, 838, 875
BstNI CCWGG 1 cut(s) 819
BstSCI CCNGG 2 cut(s) 651, 817
BstV1I GCAGC 1 cut(s) 850
BstV2I GAAGAC 2 cut(s) 38, 708
BsuRI GGCC 1 cut(s) 823
BtsIMutI CAGTG 2 cut(s) 476, 669
Cac8I GCNNGC 1 cut(s) 825
CaiI CAGNNNCTG 2 cut(s) 824, 902
Cfr13I GGNCC 2 cut(s) 815, 821
CseI GACGC 2 cut(s) 249, 776
Csp6I GTAC 3 cut(s) 123, 183, 207
CviAII CATG 2 cut(s) 692, 863
CviQI GTAC 3 cut(s) 123, 183, 207
DdeI CTNAG 5 cut(s) 154, 419, 492, 855, 903
DpnI GATC 2 cut(s) 587, 681
DpnII GATC 2 cut(s) 585, 679
Eco130I CCWWGG 1 cut(s) 882
Eco47I GGWCC 1 cut(s) 815
Eco57I CTGAAG 3 cut(s) 216, 408, 702
EcoO109I RGGNCCY 1 cut(s) 821
EcoRII CCWGG 1 cut(s) 817
EcoT14I CCWWGG 1 cut(s) 882
ErhI CCWWGG 1 cut(s) 882
FaeI CATG 2 cut(s) 695, 866
FaiI YATR 9 cut(s) 141, 143, 257, 274, 612, 693, 704, 740, 864
FatI CATG 2 cut(s) 691, 862
Fnu4HI GCNGC 1 cut(s) 839
Fsp4HI GCNGC 1 cut(s) 839
FspBI CTAG 2 cut(s) 119, 743
GluI GCNGC 1 cut(s) 839
HaeIII GGCC 1 cut(s) 823
HapII CCGG 2 cut(s) 448, 653
HgaI GACGC 2 cut(s) 249, 776
Hin1II CATG 2 cut(s) 695, 866
HindIII AAGCTT 2 cut(s) 282, 767
HinfI GANTC 6 cut(s) 149, 302, 323, 415, 713, 905
HpaII CCGG 2 cut(s) 448, 653
HphI GGTGA 2 cut(s) 239, 785
Hpy166II GTNNAC 1 cut(s) 638
Hpy188I TCNGA 6 cut(s) 388, 414, 420, 603, 721, 856
Hpy188III TCNNGA 1 cut(s) 448
Hpy8I GTNNAC 1 cut(s) 638
HpyAV CCTTC 3 cut(s) 218, 400, 725
HpyCH4III ACNGT 4 cut(s) 37, 396, 618, 664
HpyCH4V TGCA 5 cut(s) 4, 145, 431, 691, 869
HpyF10VI GCNNNNNNNGC 5 cut(s) 257, 603, 829, 838, 875
HpyF3I CTNAG 5 cut(s) 154, 419, 492, 855, 903
Hsp92II CATG 2 cut(s) 695, 866
Kpn2I TCCGGA 1 cut(s) 447
Kzo9I GATC 2 cut(s) 585, 679
LmnI GCTCC 1 cut(s) 853
Lsp1109I GCAGC 1 cut(s) 850
LweI GCATC 3 cut(s) 118, 181, 856
MaeI CTAG 2 cut(s) 119, 743
MaeIII GTNAC 3 cut(s) 261, 481, 773
MalI GATC 2 cut(s) 587, 681
MboI GATC 2 cut(s) 585, 679
MboII GAAGA 9 cut(s) 43, 74, 188, 209, 356, 401, 595, 708, 740
MluCI AATT 7 cut(s) 5, 269, 442, 532, 699, 722, 749
MlyI GAGTC 2 cut(s) 722, 914
MnlI CCTC 7 cut(s) 414, 534, 565, 571, 703, 850, 880
MroI TCCGGA 1 cut(s) 447
MroXI GAANNNNTTC 1 cut(s) 327
MseI TTAA 4 cut(s) 558, 632, 765, 913
MspA1I CMGCKG 1 cut(s) 812
MspCI CTTAAG 1 cut(s) 631
MspI CCGG 2 cut(s) 448, 653
MspR9I CCNGG 2 cut(s) 653, 819
MvaI CCWGG 1 cut(s) 819
MwoI GCNNNNNNNGC 5 cut(s) 257, 603, 829, 838, 875
NciI CCSGG 1 cut(s) 653
NdeII GATC 2 cut(s) 585, 679
NlaIII CATG 2 cut(s) 695, 866
NmeAIII GCCGAG 1 cut(s) 340
NmuCI GTSAC 2 cut(s) 261, 773
PdmI GAANNNNTTC 1 cut(s) 327
PfeI GAWTC 4 cut(s) 149, 302, 323, 415
PkrI GCNGC 1 cut(s) 840
PleI GAGTC 2 cut(s) 721, 913
PpsI GAGTC 2 cut(s) 721, 913
PsiI TTATAA 1 cut(s) 612
Psp6I CCWGG 1 cut(s) 817
PspGI CCWGG 1 cut(s) 817
PspPI GGNCC 2 cut(s) 815, 821
PstNI CAGNNNCTG 2 cut(s) 824, 902
PvuII CAGCTG 1 cut(s) 812
RsaI GTAC 3 cut(s) 124, 184, 208
RsaNI GTAC 3 cut(s) 123, 183, 207
SaqAI TTAA 4 cut(s) 558, 632, 765, 913
SatI GCNGC 1 cut(s) 839
Sau3AI GATC 2 cut(s) 585, 679
Sau96I GGNCC 2 cut(s) 815, 821
SchI GAGTC 2 cut(s) 722, 914
ScrFI CCNGG 2 cut(s) 653, 819
SfaNI GCATC 3 cut(s) 118, 181, 856
SinI GGWCC 1 cut(s) 815
SmlI CTYRAG 1 cut(s) 631
SmoI CTYRAG 1 cut(s) 631
Sse9I AATT 7 cut(s) 5, 269, 442, 532, 699, 722, 749
SspMI CTAG 2 cut(s) 119, 743
StyD4I CCNGG 2 cut(s) 651, 817
StyI CCWWGG 1 cut(s) 882
TaaI ACNGT 4 cut(s) 37, 396, 618, 664
TasI AATT 7 cut(s) 5, 269, 442, 532, 699, 722, 749
TatI WGTACW 1 cut(s) 182
TfiI GAWTC 4 cut(s) 149, 302, 323, 415
Tru1I TTAA 4 cut(s) 558, 632, 765, 913
Tru9I TTAA 4 cut(s) 558, 632, 765, 913
TscAI CASTG 2 cut(s) 483, 669
TseFI GTSAC 2 cut(s) 261, 773
TseI GCWGC 1 cut(s) 838
Tsp45I GTSAC 2 cut(s) 261, 773
TspDTI ATGAA 4 cut(s) 315, 363, 708, 762
TspGWI ACGGA 1 cut(s) 672
TspRI CASTG 2 cut(s) 483, 669
Vha464I CTTAAG 1 cut(s) 631
VpaK11BI GGWCC 1 cut(s) 815
XapI RAATTY 2 cut(s) 722, 749
XmnI GAANNNNTTC 1 cut(s) 327
XspI CTAG 2 cut(s) 119, 743
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.