MD14G1011600.v1.1

Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0004571
Physical Location & Seq
Forward (+)
1103902 .. 1108907
5006 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1011600.v1.1.491

Sequence Viewer

Length: 1032 bp
ATGGCCAACGTCTTCCGAACTTCACCATTCTCGGCGCCAATTGCACCGCCGCCCTCCGCCGCCTCGCCCAACTCTAGAAAACGTTCGCCCGTTTCATTTCGGCTAAATCAAACAAGCGGCAACAGCACGCCTTATCTCACTGTAAGCTCCCTCCGGTTCTCTCACTTGCCGTCTCTCCGGTTCGTCAAGTTCGCGCCTTTGGCTTCACAAGGTGAGACCGAGACAGCTGAGACGGTGGAGAAGGAGGTTCAGGAGGGCGAAGAAGTCGAGGACTCTTCAGATGGTGTAATTCCTGTGGAAGATAGTACAAGTGACGGTGAAGAAGGTGGTACAACTGATGATGGTGAAGGTGATACTGAGGAAAAATCTGTTTCAGCCATCACAGCTTCACTCCAACTATACAAAGAAGCTTTAGCCAGTAATGATGAATCAAAAGTTGCTGAAATAGAATCTTTCCTAAAATCTTTTGAAGATGAAAAAATAGGACTTGAAATGAAAGTGGCTTCTTTGTCTGAAGAACTGTCAGCAGAAAAGGTTCGGATTCTGAGAATAAGTGCAGATTTTGACAATTTCCGGAAAAGGACAGAAAGGGAACGCATTTCTCTCGTAACAAATGCTCAAGGGGAAGTTGTGGAGAGTTTGTTGCCTGTAGTGGATAATTTTGAGAGGGCAAAAAGCCAGATTAAGGTGGAGGCAGAGGGAGAGGAGAAGATCAACAATAGCTACCAGAGCATTTATAAACAGTTTGGAGAGATTTTAAACTCCCTTGGTGTTGTCCCTGTTGAGACGGTTGGAAAACCCTTTGATCCATTGTTGCATGAAGCAATCATGCGTGAGGACTCTTCAGAATACGAAGAAGGTATTATAATTGATGAATTTCGCAAGGGGTTTAAGCTCGGTGACAGGCTTTTGCGGCCGTCAATGGTAAAGGTATCGGCTGGTCCAGGGCCTGCCAAGGTAGAGCAGGAAGTACCTCCATCTGAGGAACAGGATGCAAGTGAAACCACCGAGAAGGGAATCGAGTCAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GrpE

Protein Analysis

344

Amino Acids

37.55

Weight (kDa)

4.54

Isoelectric Point (pI)

53.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GrpE PF01025 159 - 312 5.3e-41 GrpE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 738, 866
AccB1I GGYRCC 1 cut(s) 34
AccII CGCG 1 cut(s) 194
AccIII TCCGGA 1 cut(s) 573
AciI CCGC 6 cut(s) 47, 50, 57, 60, 117, 913
AclI AACGTT 1 cut(s) 82
AclWI GGATC 1 cut(s) 800
AcoI YGGCCR 2 cut(s) 3, 914
AcsI RAATTY 1 cut(s) 875
AcuI CTGAAG 3 cut(s) 261, 534, 828
AcyI GRCGYC 1 cut(s) 35
AdeI CACNNNGTG 1 cut(s) 212
AfaI GTAC 3 cut(s) 307, 331, 972
AfiI CCNNNNNNNGG 1 cut(s) 685
AgsI TTSAA 2 cut(s) 470, 491
AjnI CCWGG 1 cut(s) 943
AluBI AGCT 7 cut(s) 147, 227, 386, 410, 723, 895, 1028
AluI AGCT 7 cut(s) 147, 227, 386, 410, 723, 895, 1028
Alw26I GTCTC 5 cut(s) 177, 209, 215, 224, 779
AlwI GGATC 1 cut(s) 800
AlwNI CAGNNNCTG 1 cut(s) 950
Aor13HI TCCGGA 1 cut(s) 573
AoxI GGCC 3 cut(s) 3, 914, 947
ApoI RAATTY 1 cut(s) 875
Asp700I GAANNNNTTC 2 cut(s) 82, 534
AspLEI GCGC 2 cut(s) 37, 196
AspS9I GGNCC 2 cut(s) 941, 947
AsuHPI GGTGA 6 cut(s) 15, 224, 329, 356, 362, 911
AvaII GGWCC 1 cut(s) 941
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 34
BbsI GAAGAC 1 cut(s) 4
BccI CCATC 4 cut(s) 275, 335, 386, 985
BceAI ACGGC 2 cut(s) 154, 901
BcgI CGANNNNNNTGC 2 cut(s) 586, 620
BciT130I CCWGG 1 cut(s) 945
BcoDI GTCTC 5 cut(s) 177, 209, 215, 224, 779
BfaI CTAG 1 cut(s) 75
BfmI CTRYAG 1 cut(s) 648
BfoI RGCGCY 1 cut(s) 38
BisI GCNGC 4 cut(s) 50, 60, 118, 914
BlsI GCNGC 4 cut(s) 51, 61, 119, 915
Bme1390I CCNGG 1 cut(s) 945
Bme18I GGWCC 1 cut(s) 941
BmgT120I GGNCC 2 cut(s) 941, 947
BmiI GGNNCC 1 cut(s) 36
BmrFI CCNGG 1 cut(s) 945
BmsI GCATC 1 cut(s) 982
BpiI GAAGAC 1 cut(s) 4
BpuEI CTTGAG 1 cut(s) 603
BsaHI GRCGYC 1 cut(s) 35
BsaI GGTCTC 1 cut(s) 209
BsaJI CCNNGG 3 cut(s) 766, 944, 954
BsaWI WCCGGW 3 cut(s) 153, 177, 573
BsaXI ACNNNNNCTCC 2 cut(s) 626, 656
Bsc4I CCNNNNNNNGG 1 cut(s) 685
Bse1I ACTGG 1 cut(s) 417
BseAI TCCGGA 1 cut(s) 573
BseBI CCWGG 1 cut(s) 945
BseDI CCNNGG 3 cut(s) 766, 944, 954
BseGI GGATG 1 cut(s) 997
BseLI CCNNNNNNNGG 1 cut(s) 685
BseMII CTCAG 4 cut(s) 219, 348, 536, 972
BseNI ACTGG 1 cut(s) 417
BseRI GAGGAG 1 cut(s) 719
BseX3I CGGCCG 1 cut(s) 914
BsgI GTGCAG 1 cut(s) 576
Bsh1236I CGCG 1 cut(s) 194
Bsh1285I CGRYCG 1 cut(s) 917
BshFI GGCC 3 cut(s) 5, 916, 949
BshNI GGYRCC 1 cut(s) 34
BsiEI CGRYCG 1 cut(s) 917
BsiSI CCGG 3 cut(s) 154, 178, 574
BslFI GGGAC 1 cut(s) 761
BslI CCNNNNNNNGG 1 cut(s) 685
BsmAI GTCTC 5 cut(s) 177, 209, 215, 224, 779
BsmBI CGTCTC 3 cut(s) 177, 224, 779
BsmFI GGGAC 1 cut(s) 761
BsnI GGCC 3 cut(s) 5, 916, 949
Bso31I GGTCTC 1 cut(s) 209
Bsp13I TCCGGA 1 cut(s) 573
Bsp143I GATC 2 cut(s) 711, 805
BspACI CCGC 6 cut(s) 47, 50, 57, 60, 117, 913
BspANI GGCC 3 cut(s) 5, 916, 949
BspCNI CTCAG 4 cut(s) 220, 349, 537, 973
BspEI TCCGGA 1 cut(s) 573
BspFNI CGCG 1 cut(s) 194
BspLI GGNNCC 1 cut(s) 36
BspPI GGATC 1 cut(s) 800
BspT107I GGYRCC 1 cut(s) 34
BspTNI GGTCTC 1 cut(s) 209
BsrI ACTGG 1 cut(s) 417
BssECI CCNNGG 3 cut(s) 766, 944, 954
BssMI GATC 2 cut(s) 711, 805
BssNI GRCGYC 1 cut(s) 35
BssT1I CCWWGG 2 cut(s) 766, 954
Bst2UI CCWGG 1 cut(s) 945
Bst4CI ACNGT 6 cut(s) 142, 235, 317, 522, 744, 790
Bst6I CTCTTC 2 cut(s) 280, 847
BstACI GRCGYC 1 cut(s) 35
BstC8I GCNNGC 2 cut(s) 128, 951
BstDEI CTNAG 4 cut(s) 228, 357, 545, 981
BstF5I GGATG 1 cut(s) 997
BstFNI CGCG 1 cut(s) 194
BstH2I RGCGCY 1 cut(s) 38
BstHHI GCGC 2 cut(s) 37, 196
BstKTI GATC 2 cut(s) 714, 808
BstMAI GTCTC 5 cut(s) 177, 209, 215, 224, 779
BstMBI GATC 2 cut(s) 711, 805
BstMCI CGRYCG 1 cut(s) 917
BstMWI GCNNNNNNNGC 6 cut(s) 41, 123, 200, 383, 729, 913
BstNI CCWGG 1 cut(s) 945
BstSCI CCNGG 1 cut(s) 943
BstSFI CTRYAG 1 cut(s) 648
BstUI CGCG 1 cut(s) 194
BstV2I GAAGAC 1 cut(s) 4
BstZI CGGCCG 1 cut(s) 914
BsuRI GGCC 3 cut(s) 5, 916, 949
BtsCI GGATG 1 cut(s) 997
BtsIMutI CAGTG 1 cut(s) 138
Cac8I GCNNGC 2 cut(s) 128, 951
CaiI CAGNNNCTG 1 cut(s) 950
CfoI GCGC 2 cut(s) 37, 196
Cfr13I GGNCC 2 cut(s) 941, 947
Csp6I GTAC 3 cut(s) 306, 330, 971
CviAII CATG 2 cut(s) 818, 829
CviQI GTAC 3 cut(s) 306, 330, 971
DdeI CTNAG 4 cut(s) 228, 357, 545, 981
DinI GGCGCC 1 cut(s) 36
DpnI GATC 2 cut(s) 713, 807
DpnII GATC 2 cut(s) 711, 805
DraI TTTAAA 1 cut(s) 759
DraIII CACNNNGTG 1 cut(s) 212
EaeI YGGCCR 2 cut(s) 3, 914
EagI CGGCCG 1 cut(s) 914
Eam1104I CTCTTC 2 cut(s) 280, 847
EarI CTCTTC 2 cut(s) 280, 847
EciI GGCGGA 1 cut(s) 46
EclXI CGGCCG 1 cut(s) 914
Eco130I CCWWGG 2 cut(s) 766, 954
Eco31I GGTCTC 1 cut(s) 209
Eco47I GGWCC 1 cut(s) 941
Eco52I CGGCCG 1 cut(s) 914
Eco57I CTGAAG 3 cut(s) 261, 534, 828
EcoO109I RGGNCCY 1 cut(s) 947
EcoRII CCWGG 1 cut(s) 943
EcoT14I CCWWGG 2 cut(s) 766, 954
EgeI GGCGCC 1 cut(s) 36
EheI GGCGCC 1 cut(s) 36
ErhI CCWWGG 2 cut(s) 766, 954
Esp3I CGTCTC 3 cut(s) 177, 224, 779
FaeI CATG 2 cut(s) 821, 832
FaiI YATR 5 cut(s) 400, 738, 819, 830, 866
FaqI GGGAC 1 cut(s) 761
FatI CATG 2 cut(s) 817, 828
Fnu4HI GCNGC 4 cut(s) 50, 60, 118, 914
FokI GGATG 1 cut(s) 1004
Fsp4HI GCNGC 4 cut(s) 50, 60, 118, 914
FspBI CTAG 1 cut(s) 75
GlaI GCGC 2 cut(s) 36, 195
GluI GCNGC 4 cut(s) 50, 60, 118, 914
HaeII RGCGCY 1 cut(s) 38
HaeIII GGCC 3 cut(s) 5, 916, 949
HapII CCGG 3 cut(s) 154, 178, 574
HhaI GCGC 2 cut(s) 37, 196
Hin1I GRCGYC 1 cut(s) 35
Hin1II CATG 2 cut(s) 821, 832
Hin6I GCGC 2 cut(s) 35, 194
HinP1I GCGC 2 cut(s) 35, 194
HindIII AAGCTT 1 cut(s) 408
HinfI GANTC 7 cut(s) 272, 428, 449, 541, 839, 1017, 1022
HpaII CCGG 3 cut(s) 154, 178, 574
HphI GGTGA 6 cut(s) 15, 224, 329, 356, 362, 911
Hpy188I TCNGA 7 cut(s) 17, 280, 514, 540, 546, 847, 982
Hpy188III TCNNGA 3 cut(s) 75, 251, 574
HpyAV CCTTC 5 cut(s) 235, 317, 341, 851, 1006
HpyCH4III ACNGT 6 cut(s) 142, 235, 317, 522, 744, 790
HpyCH4IV ACGT 2 cut(s) 9, 82
HpyCH4V TGCA 4 cut(s) 44, 557, 817, 995
HpyF10VI GCNNNNNNNGC 6 cut(s) 41, 123, 200, 383, 729, 913
HpyF3I CTNAG 4 cut(s) 228, 357, 545, 981
HpySE526I ACGT 2 cut(s) 9, 82
Hsp92I GRCGYC 1 cut(s) 35
Hsp92II CATG 2 cut(s) 821, 832
HspAI GCGC 2 cut(s) 35, 194
KasI GGCGCC 1 cut(s) 34
Kpn2I TCCGGA 1 cut(s) 573
Kzo9I GATC 2 cut(s) 711, 805
LmnI GCTCC 1 cut(s) 152
LweI GCATC 1 cut(s) 982
MaeI CTAG 1 cut(s) 75
MaeII ACGT 2 cut(s) 9, 82
MaeIII GTNAC 3 cut(s) 311, 607, 899
MalI GATC 2 cut(s) 713, 807
MboI GATC 2 cut(s) 711, 805
MfeI CAATTG 1 cut(s) 39
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 6 cut(s) 39, 288, 568, 658, 867, 875
MluNI TGGCCA 1 cut(s) 5
Mly113I GGCGCC 1 cut(s) 35
MlyI GAGTC 3 cut(s) 266, 833, 1031
MmeI TCCRAC 2 cut(s) 418, 772
Mox20I TGGCCA 1 cut(s) 5
MroI TCCGGA 1 cut(s) 573
MroXI GAANNNNTTC 2 cut(s) 82, 534
MscI TGGCCA 1 cut(s) 5
MseI TTAA 4 cut(s) 684, 758, 891, 1030
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 227
MspI CCGG 3 cut(s) 154, 178, 574
MspR9I CCNGG 1 cut(s) 945
MunI CAATTG 1 cut(s) 39
MvaI CCWGG 1 cut(s) 945
MvnI CGCG 1 cut(s) 194
MwoI GCNNNNNNNGC 6 cut(s) 41, 123, 200, 383, 729, 913
NarI GGCGCC 1 cut(s) 35
NdeII GATC 2 cut(s) 711, 805
NlaIII CATG 2 cut(s) 821, 832
NlaIV GGNNCC 1 cut(s) 36
NmeAIII GCCGAG 1 cut(s) 11
NmuCI GTSAC 2 cut(s) 311, 899
PdmI GAANNNNTTC 2 cut(s) 82, 534
PfeI GAWTC 4 cut(s) 428, 449, 541, 1017
PkrI GCNGC 4 cut(s) 51, 61, 119, 915
PleI GAGTC 3 cut(s) 266, 833, 1030
PluTI GGCGCC 1 cut(s) 38
PpsI GAGTC 3 cut(s) 266, 833, 1030
PsiI TTATAA 2 cut(s) 738, 866
Psp1406I AACGTT 1 cut(s) 82
Psp6I CCWGG 1 cut(s) 943
PspGI CCWGG 1 cut(s) 943
PspN4I GGNNCC 1 cut(s) 36
PspPI GGNCC 2 cut(s) 941, 947
PstNI CAGNNNCTG 1 cut(s) 950
PvuII CAGCTG 1 cut(s) 227
RsaI GTAC 3 cut(s) 307, 331, 972
RsaNI GTAC 3 cut(s) 306, 330, 971
SaqAI TTAA 4 cut(s) 684, 758, 891, 1030
SatI GCNGC 4 cut(s) 50, 60, 118, 914
Sau3AI GATC 2 cut(s) 711, 805
Sau96I GGNCC 2 cut(s) 941, 947
SchI GAGTC 3 cut(s) 266, 833, 1031
ScrFI CCNGG 1 cut(s) 945
SfaNI GCATC 1 cut(s) 982
SfcI CTRYAG 1 cut(s) 648
SfoI GGCGCC 1 cut(s) 36
SinI GGWCC 1 cut(s) 941
SmlI CTYRAG 1 cut(s) 618
SmoI CTYRAG 1 cut(s) 618
Sse9I AATT 6 cut(s) 39, 288, 568, 658, 867, 875
SsiI CCGC 6 cut(s) 47, 50, 57, 60, 117, 913
SspDI GGCGCC 1 cut(s) 34
SspMI CTAG 1 cut(s) 75
StyD4I CCNGG 1 cut(s) 943
StyI CCWWGG 2 cut(s) 766, 954
TaaI ACNGT 6 cut(s) 142, 235, 317, 522, 744, 790
TaiI ACGT 2 cut(s) 12, 85
TaqI TCGA 2 cut(s) 267, 1020
TaqII GACCGA 1 cut(s) 233
TasI AATT 6 cut(s) 39, 288, 568, 658, 867, 875
TatI WGTACW 1 cut(s) 305
TauI GCSGC 4 cut(s) 52, 62, 120, 916
TfiI GAWTC 4 cut(s) 428, 449, 541, 1017
Tru1I TTAA 4 cut(s) 684, 758, 891, 1030
Tru9I TTAA 4 cut(s) 684, 758, 891, 1030
TscAI CASTG 1 cut(s) 145
TseFI GTSAC 2 cut(s) 311, 899
Tsp45I GTSAC 2 cut(s) 311, 899
TspDTI ATGAA 6 cut(s) 84, 441, 489, 509, 834, 888
TspRI CASTG 1 cut(s) 145
VpaK11BI GGWCC 1 cut(s) 941
XapI RAATTY 1 cut(s) 875
XbaI TCTAGA 1 cut(s) 74
XmnI GAANNNNTTC 2 cut(s) 82, 534
XspI CTAG 1 cut(s) 75
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.