Rw3G028180

Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0004571
Physical Location & Seq
Reverse (-)
41394096 .. 41399696
5601 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G028180.1

Sequence Viewer

Length: 876 bp
ATGGCCAATGTCCTCCGAAGTTCAGCGTTGAGGGCACCATTGGCTCCGCTTCCAACAGCTGTCTCGCCCAACTCTCCCAAACCCTTTTACGTTTCGTTTAGACGACAGAGGCATCCCATTCTCCGCTTCTCTCACTTGCCCTCCCTCCGCCTCCTCGTGCCCTCCGCTTCCCACAACCAGCACGGTGAAACCGATGCTACTGACACCCTCGATGCAGAGGAGCTTGAGGAGGATTCCTTAGACGGGGCAATTAGTGTGGAAGATAGTACAAGTGATGCTGAAGAAAGTGGTACAAGTGATGATGGGGAAGGTGATGACAAAAAACCTGTTTCAGCCATCATAGCGTCACTACAATTATACAAAGAAGCTTTAGCCAGTAATGATGAATCAAAAGTAGCCGAGATTGAATCTTTTCTAAAATCTGTTGAAGATGAAAAAATAGACCTTGAAAGGAAAGTGGCTTCTTTGTCTGAAGAACTGTCAGCAGGGAAGGTTCGGATTCTGAGGATTAGTGCAGACTTTGATAATTTTCGGAAAAGGACAGATAGAGAACGCATTTCACTGGTAACTAATGCTCAGGGGGAAGTTGTGGAGAGCTTGTTGGGTGTATTGGATAATTTTGAGAGGGCTAAATCCCAGATTAAGGTGGAGACAGAGGGAGAGGAGAAGATCAACAATAGCTATCAGAGCATTTATAAACAGTTTGGAGAAATCTTAAGTTCACTTGGCGTTCTCCCGGTGGAGACAGTGGGGAAGCCCTTTGATCCGTTGGTTAGATACCAAGTTGGCTCGAAATTCAATCAATTACTTGATGAACTTTATCAAGTAGTTAGTCAATTTTGTTCAGGTTTGATGAAGACCTGGATACGGTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GrpE

Protein Analysis

291

Amino Acids

32.32

Weight (kDa)

4.84

Isoelectric Point (pI)

44.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GrpE PF01025 143 - 261 7.8e-21 GrpE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 696
AccB1I GGYRCC 1 cut(s) 34
AciI CCGC 4 cut(s) 47, 124, 148, 165
AclWI GGATC 1 cut(s) 758
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 794
AcuI CTGAAG 2 cut(s) 300, 492
AfaI GTAC 2 cut(s) 268, 292
AfiI CCNNNNNNNGG 3 cut(s) 243, 643, 867
AflII CTTAAG 1 cut(s) 715
AgsI TTSAA 4 cut(s) 407, 428, 449, 799
AjnI CCWGG 1 cut(s) 860
AluBI AGCT 5 cut(s) 59, 223, 368, 597, 681
AluI AGCT 5 cut(s) 59, 223, 368, 597, 681
Alw26I GTCTC 3 cut(s) 67, 644, 737
AlwI GGATC 1 cut(s) 758
AoxI GGCC 1 cut(s) 3
ApoI RAATTY 1 cut(s) 794
Asp700I GAANNNNTTC 1 cut(s) 411
AsuC2I CCSGG 1 cut(s) 737
AsuHPI GGTGA 2 cut(s) 197, 323
BaeGI GKGCMC 2 cut(s) 37, 162
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 34
BauI CACGAG 1 cut(s) 155
BbsI GAAGAC 1 cut(s) 863
BccI CCATC 2 cut(s) 296, 344
BciT130I CCWGG 1 cut(s) 862
BciVI GTATCC 1 cut(s) 858
BcnI CCSGG 1 cut(s) 737
BcoDI GTCTC 3 cut(s) 67, 644, 737
BfrI CTTAAG 1 cut(s) 715
BfuI GTATCC 1 cut(s) 858
Bme1390I CCNGG 2 cut(s) 737, 862
BmiI GGNNCC 2 cut(s) 36, 45
BmrFI CCNGG 2 cut(s) 737, 862
BmsI GCATC 4 cut(s) 121, 184, 202, 265
BpiI GAAGAC 1 cut(s) 863
Bpu10I CCTNAGC 1 cut(s) 576
BpuEI CTTGAG 1 cut(s) 245
BpuMI CCSGG 1 cut(s) 737
BsaXI ACNNNNNCTCC 4 cut(s) 125, 155, 584, 614
Bsc4I CCNNNNNNNGG 3 cut(s) 243, 643, 867
Bse1I ACTGG 2 cut(s) 375, 567
BseBI CCWGG 1 cut(s) 862
BseGI GGATG 1 cut(s) 112
BseLI CCNNNNNNNGG 3 cut(s) 243, 643, 867
BseMII CTCAG 2 cut(s) 494, 590
BseNI ACTGG 2 cut(s) 375, 567
BseRI GAGGAG 4 cut(s) 143, 233, 242, 677
BseSI GKGCMC 2 cut(s) 37, 162
BsgI GTGCAG 1 cut(s) 534
BshFI GGCC 1 cut(s) 5
BshNI GGYRCC 1 cut(s) 34
BsiSI CCGG 1 cut(s) 737
BslI CCNNNNNNNGG 3 cut(s) 243, 643, 867
BsmAI GTCTC 3 cut(s) 67, 644, 737
BsnI GGCC 1 cut(s) 5
Bsp1286I GDGCHC 2 cut(s) 37, 162
Bsp143I GATC 2 cut(s) 669, 763
BspACI CCGC 4 cut(s) 47, 124, 148, 165
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 2 cut(s) 495, 589
BspLI GGNNCC 2 cut(s) 36, 45
BspPI GGATC 1 cut(s) 758
BspT107I GGYRCC 1 cut(s) 34
BspTI CTTAAG 1 cut(s) 715
BsrI ACTGG 2 cut(s) 375, 567
BssMI GATC 2 cut(s) 669, 763
BssSI CACGAG 1 cut(s) 155
Bst2BI CACGAG 1 cut(s) 155
Bst2UI CCWGG 1 cut(s) 862
Bst4CI ACNGT 5 cut(s) 185, 480, 702, 748, 870
BstAFI CTTAAG 1 cut(s) 715
BstDEI CTNAG 3 cut(s) 238, 503, 576
BstF5I GGATG 1 cut(s) 112
BstKTI GATC 2 cut(s) 672, 766
BstMAI GTCTC 3 cut(s) 67, 644, 737
BstMBI GATC 2 cut(s) 669, 763
BstMWI GCNNNNNNNGC 4 cut(s) 32, 41, 341, 687
BstNI CCWGG 1 cut(s) 862
BstSCI CCNGG 2 cut(s) 735, 860
BstSLI GKGCMC 2 cut(s) 37, 162
BstV2I GAAGAC 1 cut(s) 863
BsuI GTATCC 1 cut(s) 858
BsuRI GGCC 1 cut(s) 5
BtsCI GGATG 1 cut(s) 112
BtsIMutI CAGTG 2 cut(s) 560, 753
CseI GACGC 1 cut(s) 333
Csp6I GTAC 2 cut(s) 267, 291
CspCI CAANNNNNGTGG 2 cut(s) 237, 272
CviQI GTAC 2 cut(s) 267, 291
DdeI CTNAG 3 cut(s) 238, 503, 576
DpnI GATC 2 cut(s) 671, 765
DpnII GATC 2 cut(s) 669, 763
EaeI YGGCCR 1 cut(s) 3
EciI GGCGGA 1 cut(s) 137
Eco57I CTGAAG 2 cut(s) 300, 492
EcoRII CCWGG 1 cut(s) 860
FaiI YATR 3 cut(s) 341, 358, 696
FokI GGATG 1 cut(s) 99
HaeIII GGCC 1 cut(s) 5
HapII CCGG 1 cut(s) 737
HgaI GACGC 1 cut(s) 333
HindIII AAGCTT 1 cut(s) 366
HinfI GANTC 4 cut(s) 233, 386, 407, 499
HpaII CCGG 1 cut(s) 737
HphI GGTGA 2 cut(s) 197, 323
Hpy166II GTNNAC 1 cut(s) 722
Hpy188I TCNGA 6 cut(s) 17, 472, 498, 504, 534, 687
Hpy8I GTNNAC 1 cut(s) 722
HpyAV CCTTC 2 cut(s) 302, 484
HpyCH4III ACNGT 5 cut(s) 185, 480, 702, 748, 870
HpyCH4IV ACGT 1 cut(s) 90
HpyCH4V TGCA 2 cut(s) 215, 515
HpyF10VI GCNNNNNNNGC 4 cut(s) 32, 41, 341, 687
HpyF3I CTNAG 3 cut(s) 238, 503, 576
HpySE526I ACGT 1 cut(s) 90
Kzo9I GATC 2 cut(s) 669, 763
LmnI GCTCC 2 cut(s) 49, 220
LweI GCATC 4 cut(s) 121, 184, 202, 265
MaeII ACGT 1 cut(s) 90
MaeIII GTNAC 2 cut(s) 345, 565
MalI GATC 2 cut(s) 671, 765
MboI GATC 2 cut(s) 669, 763
MboII GAAGA 6 cut(s) 272, 293, 440, 485, 679, 868
MhlI GDGCHC 2 cut(s) 37, 162
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 7 cut(s) 249, 353, 526, 616, 794, 803, 836
MluNI TGGCCA 1 cut(s) 5
MmeI TCCRAC 1 cut(s) 77
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 1 cut(s) 411
MscI TGGCCA 1 cut(s) 5
MseI TTAA 3 cut(s) 642, 716, 874
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 59
MspCI CTTAAG 1 cut(s) 715
MspI CCGG 1 cut(s) 737
MspR9I CCNGG 2 cut(s) 737, 862
MvaI CCWGG 1 cut(s) 862
MwoI GCNNNNNNNGC 4 cut(s) 32, 41, 341, 687
NciI CCSGG 1 cut(s) 737
NdeII GATC 2 cut(s) 669, 763
NlaIV GGNNCC 2 cut(s) 36, 45
NmeAIII GCCGAG 1 cut(s) 424
NmuCI GTSAC 1 cut(s) 345
PcsI WCGNNNNNNNCGW 1 cut(s) 189
PdmI GAANNNNTTC 1 cut(s) 411
PfeI GAWTC 4 cut(s) 233, 386, 407, 499
PsiI TTATAA 1 cut(s) 696
Psp6I CCWGG 1 cut(s) 860
PspGI CCWGG 1 cut(s) 860
PspN4I GGNNCC 2 cut(s) 36, 45
PvuII CAGCTG 1 cut(s) 59
RsaI GTAC 2 cut(s) 268, 292
RsaNI GTAC 2 cut(s) 267, 291
SaqAI TTAA 3 cut(s) 642, 716, 874
Sau3AI GATC 2 cut(s) 669, 763
ScrFI CCNGG 2 cut(s) 737, 862
SduI GDGCHC 2 cut(s) 37, 162
SfaNI GCATC 4 cut(s) 121, 184, 202, 265
SmlI CTYRAG 2 cut(s) 224, 715
SmoI CTYRAG 2 cut(s) 224, 715
Sse9I AATT 7 cut(s) 249, 353, 526, 616, 794, 803, 836
SsiI CCGC 4 cut(s) 47, 124, 148, 165
StyD4I CCNGG 2 cut(s) 735, 860
TaaI ACNGT 5 cut(s) 185, 480, 702, 748, 870
TaiI ACGT 1 cut(s) 93
TaqI TCGA 2 cut(s) 210, 791
TasI AATT 7 cut(s) 249, 353, 526, 616, 794, 803, 836
TatI WGTACW 1 cut(s) 266
TfiI GAWTC 4 cut(s) 233, 386, 407, 499
Tru1I TTAA 3 cut(s) 642, 716, 874
Tru9I TTAA 3 cut(s) 642, 716, 874
TscAI CASTG 2 cut(s) 567, 753
TseFI GTSAC 1 cut(s) 345
Tsp45I GTSAC 1 cut(s) 345
TspDTI ATGAA 4 cut(s) 399, 447, 828, 869
TspGWI ACGGA 1 cut(s) 756
TspRI CASTG 2 cut(s) 567, 753
Vha464I CTTAAG 1 cut(s) 715
XapI RAATTY 1 cut(s) 794
XmnI GAANNNNTTC 1 cut(s) 411
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.