AT5G19430

Ring finger domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0007205
Physical Location & Seq
Forward (+)
6553703 .. 6555954
2252 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G19430.2

Sequence Viewer

Length: 768 bp
ATGAGTTCCGTTACCGAGATCGTGTCTGAGCAGCAAGTGATCGACGGTATTCAGGATTTGGCCCTTCAAAATCAGGACAAAAAGAAGATCCAGGAAGAGATCCATGAGCCGAGTTTCGGGAATCATGGTGGCTGTTGTGCAATATGTTTGAGTGAAATTCCTCTTCAAGAAACTGCTATGGTCAAAGGCTGTGAACACACTTACTGTGTGACATGCATACTTCGTTGGGCGAGTTGCAAAGAGAGTCCCACATGCCCACAATGTAAGCATCCATTTGATTTTCTCAGTGTCCACCGAGCACTTGATGGCAGCATTGAAGATTTTCTGTTTGAGGAGAGTGTGTGCCTTCTCCTTAGAGCCTCATGGTTTGTACCATTGGATGTGGTGGAACAGGCGTCGTACAGCCATGGTTACCATGATGATTTTGACATTCCTTATGACTACGAGGATGAAGATGATGACCTTGACGAGTTTTATCTGCACGGCTCAAGTCTTCGCATAGGAAATCGGAGATGGGGTGGCAATGGTTTTGTCAGATCCGGGCGTCAAGAAGCCAGGCCTGTCCAGAGATACAGTGGTTCTGGTTCTAGTTCCAGTTCCAGCTCTGGGTCATCCTCAAGTGAGCCTAAGGATAAGCAGGTGAAGACGACAAACACAACAGGAAGACGTGCAAAAAGGGCAATGAAGCGTGAAGCTGCAAACAAAGCAGCGGAAGTAACTGCAGCAGCAAAGCACGAGGCCCATTTGGTTAGGTTGGGAAGGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

255

Amino Acids

28.4

Weight (kDa)

5.7

Isoelectric Point (pI)

64.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 30 - 74 6e-07 Ring finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 628
Acc36I ACCTGC 1 cut(s) 628
AciI CCGC 1 cut(s) 710
AclWI GGATC 3 cut(s) 82, 94, 531
AcsI RAATTY 1 cut(s) 156
AcyI GRCGYC 2 cut(s) 395, 544
AfaI GTAC 2 cut(s) 372, 401
AfiI CCNNNNNNNGG 2 cut(s) 116, 606
AgsI TTSAA 3 cut(s) 68, 167, 317
AjiI CACGTC 1 cut(s) 668
AjnI CCWGG 2 cut(s) 90, 554
AluBI AGCT 2 cut(s) 603, 695
AluI AGCT 2 cut(s) 603, 695
Alw21I GWGCWC 1 cut(s) 301
AlwI GGATC 3 cut(s) 82, 94, 531
AoxI GGCC 3 cut(s) 60, 557, 738
ApeKI GCWGC 6 cut(s) 31, 309, 695, 707, 722, 725
ApoI RAATTY 1 cut(s) 156
Asp700I GAANNNNTTC 1 cut(s) 321
AspS9I GGNCC 2 cut(s) 61, 739
AsuC2I CCSGG 1 cut(s) 541
AsuHPI GGTGA 1 cut(s) 652
AxyI CCTNAGG 1 cut(s) 627
BaeI ACNNNNGTAYC 2 cut(s) 562, 595
BauI CACGAG 1 cut(s) 734
BbsI GAAGAC 3 cut(s) 485, 650, 670
Bbv12I GWGCWC 1 cut(s) 301
BbvI GCAGC 6 cut(s) 43, 321, 682, 719, 734, 737
BccI CCATC 2 cut(s) 299, 507
BceAI ACGGC 1 cut(s) 499
BciT130I CCWGG 2 cut(s) 92, 556
BcnI CCSGG 1 cut(s) 541
BfaI CTAG 1 cut(s) 588
BfmI CTRYAG 1 cut(s) 720
BfuAI ACCTGC 1 cut(s) 628
BisI GCNGC 6 cut(s) 32, 310, 696, 708, 723, 726
BlsI GCNGC 6 cut(s) 33, 311, 697, 709, 724, 727
Bme1390I CCNGG 3 cut(s) 92, 541, 556
BmgBI CACGTC 1 cut(s) 668
BmgT120I GGNCC 2 cut(s) 61, 739
BmrFI CCNGG 3 cut(s) 92, 541, 556
BmsI GCATC 1 cut(s) 277
BpiI GAAGAC 3 cut(s) 485, 650, 670
BpuEI CTTGAG 2 cut(s) 472, 601
BpuMI CCSGG 1 cut(s) 541
BsaHI GRCGYC 2 cut(s) 395, 544
BsaJI CCNNGG 1 cut(s) 406
BsaXI ACNNNNNCTCC 4 cut(s) 326, 356, 502, 532
Bsc4I CCNNNNNNNGG 2 cut(s) 116, 606
Bse1I ACTGG 1 cut(s) 594
Bse21I CCTNAGG 1 cut(s) 627
Bse3DI GCAATG 2 cut(s) 529, 687
BseBI CCWGG 2 cut(s) 92, 556
BseDI CCNNGG 1 cut(s) 406
BseGI GGATG 4 cut(s) 268, 385, 454, 611
BseLI CCNNNNNNNGG 2 cut(s) 116, 606
BseMI GCAATG 2 cut(s) 529, 687
BseMII CTCAG 2 cut(s) 18, 298
BseNI ACTGG 1 cut(s) 594
BseRI GAGGAG 1 cut(s) 347
BseXI GCAGC 6 cut(s) 43, 321, 682, 719, 734, 737
BsgI GTGCAG 1 cut(s) 464
BshFI GGCC 3 cut(s) 62, 559, 740
BsiHKAI GWGCWC 1 cut(s) 301
BsiSI CCGG 1 cut(s) 540
BslFI GGGAC 1 cut(s) 231
BslI CCNNNNNNNGG 2 cut(s) 116, 606
BsmFI GGGAC 1 cut(s) 231
BsnI GGCC 3 cut(s) 62, 559, 740
Bsp1286I GDGCHC 1 cut(s) 301
Bsp143I GATC 5 cut(s) 18, 39, 87, 99, 536
Bsp19I CCATGG 1 cut(s) 406
BspACI CCGC 1 cut(s) 710
BspANI GGCC 3 cut(s) 62, 559, 740
BspCNI CTCAG 2 cut(s) 19, 297
BspMAI CTGCAG 1 cut(s) 724
BspMI ACCTGC 1 cut(s) 628
BspPI GGATC 3 cut(s) 82, 94, 531
BsrDI GCAATG 2 cut(s) 529, 687
BsrI ACTGG 1 cut(s) 594
BssECI CCNNGG 1 cut(s) 406
BssMI GATC 5 cut(s) 18, 39, 87, 99, 536
BssNI GRCGYC 2 cut(s) 395, 544
BssSI CACGAG 1 cut(s) 734
BssT1I CCWWGG 1 cut(s) 406
Bst2BI CACGAG 1 cut(s) 734
Bst2UI CCWGG 2 cut(s) 92, 556
Bst4CI ACNGT 3 cut(s) 47, 206, 575
Bst6I CTCTTC 2 cut(s) 90, 168
BstACI GRCGYC 2 cut(s) 395, 544
BstDEI CTNAG 4 cut(s) 27, 284, 353, 627
BstDSI CCRYGG 1 cut(s) 406
BstEII GGTNACC 1 cut(s) 410
BstF5I GGATG 4 cut(s) 268, 385, 454, 611
BstKTI GATC 5 cut(s) 21, 42, 90, 102, 539
BstMBI GATC 5 cut(s) 18, 39, 87, 99, 536
BstMWI GCNNNNNNNGC 2 cut(s) 677, 704
BstNI CCWGG 2 cut(s) 92, 556
BstNSI RCATGY 2 cut(s) 216, 255
BstPI GGTNACC 1 cut(s) 410
BstSCI CCNGG 3 cut(s) 90, 539, 554
BstSFI CTRYAG 1 cut(s) 720
BstV1I GCAGC 6 cut(s) 43, 321, 682, 719, 734, 737
BstV2I GAAGAC 3 cut(s) 485, 650, 670
BstX2I RGATCY 3 cut(s) 87, 99, 536
BstYI RGATCY 3 cut(s) 87, 99, 536
Bsu36I CCTNAGG 1 cut(s) 627
BsuRI GGCC 3 cut(s) 62, 559, 740
BtgI CCRYGG 1 cut(s) 406
BtrI CACGTC 1 cut(s) 668
BtsCI GGATG 4 cut(s) 268, 385, 454, 611
BtsIMutI CAGTG 2 cut(s) 292, 580
BveI ACCTGC 1 cut(s) 628
Cfr13I GGNCC 2 cut(s) 61, 739
CseI GACGC 2 cut(s) 384, 533
Csp6I GTAC 2 cut(s) 371, 400
CviAII CATG 7 cut(s) 104, 125, 213, 252, 363, 407, 416
CviQI GTAC 2 cut(s) 371, 400
DdeI CTNAG 4 cut(s) 27, 284, 353, 627
DpnI GATC 5 cut(s) 20, 41, 89, 101, 538
DpnII GATC 5 cut(s) 18, 39, 87, 99, 536
Eam1104I CTCTTC 2 cut(s) 90, 168
EarI CTCTTC 2 cut(s) 90, 168
Eco130I CCWWGG 1 cut(s) 406
Eco147I AGGCCT 1 cut(s) 559
Eco81I CCTNAGG 1 cut(s) 627
Eco91I GGTNACC 1 cut(s) 410
EcoO65I GGTNACC 1 cut(s) 410
EcoRII CCWGG 2 cut(s) 90, 554
EcoT14I CCWWGG 1 cut(s) 406
EcoT22I ATGCAT 1 cut(s) 218
ErhI CCWWGG 1 cut(s) 406
FaeI CATG 7 cut(s) 107, 128, 216, 255, 366, 410, 419
FaqI GGGAC 1 cut(s) 231
FatI CATG 7 cut(s) 103, 124, 212, 251, 362, 406, 415
Fnu4HI GCNGC 6 cut(s) 32, 310, 696, 708, 723, 726
FokI GGATG 4 cut(s) 255, 392, 461, 598
Fsp4HI GCNGC 6 cut(s) 32, 310, 696, 708, 723, 726
FspBI CTAG 1 cut(s) 588
GluI GCNGC 6 cut(s) 32, 310, 696, 708, 723, 726
HaeIII GGCC 3 cut(s) 62, 559, 740
HapII CCGG 1 cut(s) 540
HgaI GACGC 2 cut(s) 384, 533
Hin1I GRCGYC 2 cut(s) 395, 544
Hin1II CATG 7 cut(s) 107, 128, 216, 255, 366, 410, 419
HinfI GANTC 2 cut(s) 121, 244
HpaII CCGG 1 cut(s) 540
HphI GGTGA 1 cut(s) 652
Hpy166II GTNNAC 2 cut(s) 194, 292
Hpy188I TCNGA 3 cut(s) 28, 510, 536
Hpy188III TCNNGA 6 cut(s) 53, 74, 118, 167, 548, 565
Hpy8I GTNNAC 2 cut(s) 194, 292
Hpy99I CGWCG 2 cut(s) 47, 400
HpyAV CCTTC 3 cut(s) 74, 356, 753
HpyCH4III ACNGT 3 cut(s) 47, 206, 575
HpyCH4IV ACGT 1 cut(s) 667
HpyCH4V TGCA 7 cut(s) 140, 216, 237, 481, 671, 698, 722
HpyF10VI GCNNNNNNNGC 2 cut(s) 677, 704
HpyF3I CTNAG 4 cut(s) 27, 284, 353, 627
HpySE526I ACGT 1 cut(s) 667
Hsp92I GRCGYC 2 cut(s) 395, 544
Hsp92II CATG 7 cut(s) 107, 128, 216, 255, 366, 410, 419
Kzo9I GATC 5 cut(s) 18, 39, 87, 99, 536
Lsp1109I GCAGC 6 cut(s) 43, 321, 682, 719, 734, 737
LweI GCATC 1 cut(s) 277
MaeI CTAG 1 cut(s) 588
MaeII ACGT 1 cut(s) 667
MaeIII GTNAC 4 cut(s) 10, 208, 410, 715
MalI GATC 5 cut(s) 20, 41, 89, 101, 538
MboI GATC 5 cut(s) 18, 39, 87, 99, 536
MboII GAAGA 8 cut(s) 97, 107, 155, 329, 464, 485, 655, 675
MflI RGATCY 3 cut(s) 87, 99, 536
MhlI GDGCHC 1 cut(s) 301
MluCI AATT 1 cut(s) 156
MlyI GAGTC 1 cut(s) 253
MnlI CCTC 6 cut(s) 171, 325, 370, 439, 625, 730
Mph1103I ATGCAT 1 cut(s) 218
MroXI GAANNNNTTC 1 cut(s) 321
MspA1I CMGCKG 1 cut(s) 710
MspI CCGG 1 cut(s) 540
MspR9I CCNGG 3 cut(s) 92, 541, 556
MvaI CCWGG 2 cut(s) 92, 556
MwoI GCNNNNNNNGC 2 cut(s) 677, 704
NciI CCSGG 1 cut(s) 541
NcoI CCATGG 1 cut(s) 406
NdeII GATC 5 cut(s) 18, 39, 87, 99, 536
NlaIII CATG 7 cut(s) 107, 128, 216, 255, 366, 410, 419
NmeAIII GCCGAG 1 cut(s) 135
NmuCI GTSAC 1 cut(s) 208
NsiI ATGCAT 1 cut(s) 218
NspI RCATGY 2 cut(s) 216, 255
PaqCI CACCTGC 1 cut(s) 628
PceI AGGCCT 1 cut(s) 559
PdmI GAANNNNTTC 1 cut(s) 321
PfeI GAWTC 1 cut(s) 121
PfoI TCCNGGA 1 cut(s) 90
PkrI GCNGC 6 cut(s) 33, 311, 697, 709, 724, 727
PleI GAGTC 1 cut(s) 252
PpsI GAGTC 1 cut(s) 252
Psp6I CCWGG 2 cut(s) 90, 554
PspEI GGTNACC 1 cut(s) 410
PspGI CCWGG 2 cut(s) 90, 554
PspPI GGNCC 2 cut(s) 61, 739
PstI CTGCAG 1 cut(s) 724
PsuI RGATCY 3 cut(s) 87, 99, 536
RsaI GTAC 2 cut(s) 372, 401
RsaNI GTAC 2 cut(s) 371, 400
SatI GCNGC 6 cut(s) 32, 310, 696, 708, 723, 726
Sau3AI GATC 5 cut(s) 18, 39, 87, 99, 536
Sau96I GGNCC 2 cut(s) 61, 739
SchI GAGTC 1 cut(s) 253
ScrFI CCNGG 3 cut(s) 92, 541, 556
SduI GDGCHC 1 cut(s) 301
SetI ASST 6 cut(s) 465, 605, 642, 670, 697, 755
SfaNI GCATC 1 cut(s) 277
SfcI CTRYAG 1 cut(s) 720
SmlI CTYRAG 2 cut(s) 487, 616
SmoI CTYRAG 2 cut(s) 487, 616
Sse9I AATT 1 cut(s) 156
SseBI AGGCCT 1 cut(s) 559
SsiI CCGC 1 cut(s) 710
SspMI CTAG 1 cut(s) 588
StuI AGGCCT 1 cut(s) 559
StyD4I CCNGG 3 cut(s) 90, 539, 554
StyI CCWWGG 1 cut(s) 406
TaaI ACNGT 3 cut(s) 47, 206, 575
TaiI ACGT 1 cut(s) 670
TaqI TCGA 1 cut(s) 42
TasI AATT 1 cut(s) 156
TfiI GAWTC 1 cut(s) 121
TscAI CASTG 2 cut(s) 292, 580
TseFI GTSAC 1 cut(s) 208
TseI GCWGC 6 cut(s) 31, 309, 695, 707, 722, 725
Tsp45I GTSAC 1 cut(s) 208
TspDTI ATGAA 2 cut(s) 465, 698
TspRI CASTG 2 cut(s) 292, 580
XapI RAATTY 1 cut(s) 156
XceI RCATGY 2 cut(s) 216, 255
XcmI CCANNNNNNNNNTGG 1 cut(s) 572
XmnI GAANNNNTTC 1 cut(s) 321
XspI CTAG 1 cut(s) 588
Zsp2I ATGCAT 1 cut(s) 218
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.