Rw5G031560

Zinc finger, C3HC4 type (RING finger)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0007205
Physical Location & Seq
Reverse (-)
52201337 .. 52204444
3108 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G031560.1

Sequence Viewer

Length: 792 bp
ATGATTCAAGTTAGATCAGTAGAAAAACTTAGGGAGGTTATCAAATCGATCATGGCTTCAGTCATCAACGTCGACGGCCACGATCACCTACCCTTGGATCTTTGCAATTCATCCACCCATCAACACCAGGGGGAAATGGAAAGCAAAGAAGATAATTGTGAAAAGGGGCGATGTGGGAACCATGGTGGGGTGTGTGCAATATGTTTGGATGAGATTATGCTTCAGGAAACGGCTCTTGTAAAAGGTTGCGAGCATGCCTACTGTGCAACTTGCATTCTTCGTTGGGTGACATACAGCAAATCACCCACCTGCCCTCAGTGTAAACATCCATTCGAGTTCCTGAATGTCCATCGGTCACTTGATGGCAGCATACATGATTTTATGTTCGAGGAGAGTGTGTGTCTACTGCTTAGAGCCACATGGTTTGAGCCTCTAGTTGTGGAGGATCGCGAAGATGTGTATGCATATGATGATTCTGAAGATTATTATCCCTACGAGGATGAAGAGGATGACCTAGATGAAGCTTACTTCAGCAGTTCATTGAGTATCCGTATTGGTAATCGAAGGTGGGGAGATAATGGGTATGTGAGCGCTGGACGGCAAGAAGCAAGGCCTGTTCACAGAACAAATATCCAGGAAGCAGGTGCTAGTTCTTCTCGTGAACCTAGAAAGAAAGAGGCTTCAATGGATAAAACTGGGAGACGGGCAAAGAGGGCACTTAAGCGAGAAGCTGCGGATAAGGCTGCTGCAGCAAAACATGAGCTGCATTTGGCAAGGTTGGGCCGGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4 zf-RING_2

Protein Analysis

263

Amino Acids

29.88

Weight (kDa)

5.76

Isoelectric Point (pI)

50.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 65 - 108 6.9e-10 Ring finger domain
zf-C3HC4_2 PF13923 65 - 107 5.5e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 65 - 107 3.7e-07 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 317, 632
Acc36I ACCTGC 2 cut(s) 317, 632
AccI GTMKAC 2 cut(s) 72, 403
AccII CGCG 1 cut(s) 450
AciI CCGC 1 cut(s) 734
AclWI GGATC 2 cut(s) 105, 453
AcoI YGGCCR 1 cut(s) 76
AcuI CTGAAG 4 cut(s) 42, 206, 498, 514
AfeI AGCGCT 1 cut(s) 592
AfiI CCNNNNNNNGG 2 cut(s) 94, 187
AflII CTTAAG 1 cut(s) 719
AgsI TTSAA 2 cut(s) 8, 684
AjnI CCWGG 2 cut(s) 126, 633
AluBI AGCT 3 cut(s) 524, 731, 763
AluI AGCT 3 cut(s) 524, 731, 763
Alw26I GTCTC 1 cut(s) 694
AlwI GGATC 2 cut(s) 105, 453
Aor51HI AGCGCT 1 cut(s) 592
AoxI GGCC 3 cut(s) 76, 611, 781
ApeKI GCWGC 6 cut(s) 366, 731, 743, 746, 749, 763
AspLEI GCGC 1 cut(s) 593
AspS9I GGNCC 1 cut(s) 781
AsuHPI GGTGA 3 cut(s) 77, 294, 298
BaeGI GKGCMC 1 cut(s) 718
BauI CACGAG 1 cut(s) 657
BbvI GCAGC 6 cut(s) 378, 718, 730, 733, 750, 761
BccI CCATC 3 cut(s) 126, 356, 357
BceAI ACGGC 3 cut(s) 91, 246, 614
BciT130I CCWGG 2 cut(s) 128, 635
BciVI GTATCC 1 cut(s) 557
BcoDI GTCTC 1 cut(s) 694
BfaI CTAG 4 cut(s) 434, 515, 648, 666
BfmI CTRYAG 1 cut(s) 747
BfoI RGCGCY 1 cut(s) 594
BfrI CTTAAG 1 cut(s) 719
BfuAI ACCTGC 2 cut(s) 317, 632
BfuI GTATCC 1 cut(s) 557
BisI GCNGC 6 cut(s) 367, 732, 744, 747, 750, 764
BlsI GCNGC 6 cut(s) 368, 733, 745, 748, 751, 765
Bme1390I CCNGG 2 cut(s) 128, 635
BmgT120I GGNCC 1 cut(s) 781
BmiI GGNNCC 1 cut(s) 179
BmrFI CCNGG 2 cut(s) 128, 635
BmrI ACTGGG 1 cut(s) 705
BmuI ACTGGG 1 cut(s) 705
Bsa29I ATCGAT 1 cut(s) 47
BsaBI GATNNNNATC 1 cut(s) 486
BsaJI CCNNGG 3 cut(s) 93, 127, 181
BsaXI ACNNNNNCTCC 2 cut(s) 383, 413
Bsc4I CCNNNNNNNGG 2 cut(s) 94, 187
Bse1I ACTGG 1 cut(s) 700
Bse8I GATNNNNATC 1 cut(s) 486
BseBI CCWGG 2 cut(s) 128, 635
BseCI ATCGAT 1 cut(s) 47
BseDI CCNNGG 3 cut(s) 93, 127, 181
BseGI GGATG 5 cut(s) 110, 214, 325, 505, 514
BseJI GATNNNNATC 1 cut(s) 486
BseLI CCNNNNNNNGG 2 cut(s) 94, 187
BseMII CTCAG 1 cut(s) 329
BseNI ACTGG 1 cut(s) 700
BseRI GAGGAG 1 cut(s) 404
BseSI GKGCMC 1 cut(s) 718
BseXI GCAGC 6 cut(s) 378, 718, 730, 733, 750, 761
Bsh1236I CGCG 1 cut(s) 450
BshFI GGCC 3 cut(s) 78, 613, 783
BshVI ATCGAT 1 cut(s) 47
BsiSI CCGG 1 cut(s) 784
BslI CCNNNNNNNGG 2 cut(s) 94, 187
BsmAI GTCTC 1 cut(s) 694
BsmBI CGTCTC 1 cut(s) 694
BsmI GAATGC 1 cut(s) 273
BsnI GGCC 3 cut(s) 78, 613, 783
Bsp1286I GDGCHC 1 cut(s) 718
Bsp143I GATC 5 cut(s) 14, 48, 82, 97, 445
Bsp19I CCATGG 1 cut(s) 181
Bsp68I TCGCGA 1 cut(s) 450
BspACI CCGC 1 cut(s) 734
BspANI GGCC 3 cut(s) 78, 613, 783
BspCNI CTCAG 1 cut(s) 328
BspDI ATCGAT 1 cut(s) 47
BspFNI CGCG 1 cut(s) 450
BspLI GGNNCC 1 cut(s) 179
BspMAI CTGCAG 1 cut(s) 751
BspMI ACCTGC 2 cut(s) 317, 632
BspPI GGATC 2 cut(s) 105, 453
BspTI CTTAAG 1 cut(s) 719
BsrI ACTGG 1 cut(s) 700
BssECI CCNNGG 3 cut(s) 93, 127, 181
BssMI GATC 5 cut(s) 14, 48, 82, 97, 445
BssSI CACGAG 1 cut(s) 657
BssT1I CCWWGG 2 cut(s) 93, 181
Bst2BI CACGAG 1 cut(s) 657
Bst2UI CCWGG 2 cut(s) 128, 635
Bst4CI ACNGT 1 cut(s) 263
Bst6I CTCTTC 1 cut(s) 498
BstAFI CTTAAG 1 cut(s) 719
BstC8I GCNNGC 2 cut(s) 251, 255
BstDEI CTNAG 3 cut(s) 29, 315, 410
BstDSI CCRYGG 1 cut(s) 181
BstF5I GGATG 5 cut(s) 110, 214, 325, 505, 514
BstFNI CGCG 1 cut(s) 450
BstH2I RGCGCY 1 cut(s) 594
BstHHI GCGC 1 cut(s) 593
BstKTI GATC 5 cut(s) 17, 51, 85, 100, 448
BstMAI GTCTC 1 cut(s) 694
BstMBI GATC 5 cut(s) 14, 48, 82, 97, 445
BstMWI GCNNNNNNNGC 4 cut(s) 263, 713, 740, 749
BstNI CCWGG 2 cut(s) 128, 635
BstNSI RCATGY 1 cut(s) 257
BstSCI CCNGG 2 cut(s) 126, 633
BstSFI CTRYAG 1 cut(s) 747
BstSLI GKGCMC 1 cut(s) 718
BstUI CGCG 1 cut(s) 450
BstV1I GCAGC 6 cut(s) 378, 718, 730, 733, 750, 761
BstX2I RGATCY 1 cut(s) 97
BstYI RGATCY 1 cut(s) 97
Bsu15I ATCGAT 1 cut(s) 47
BsuI GTATCC 1 cut(s) 557
BsuRI GGCC 3 cut(s) 78, 613, 783
BsuTUI ATCGAT 1 cut(s) 47
BtgI CCRYGG 1 cut(s) 181
BtgZI GCGATG 1 cut(s) 184
BtsCI GGATG 5 cut(s) 110, 214, 325, 505, 514
BtsIMutI CAGTG 1 cut(s) 323
BtuMI TCGCGA 1 cut(s) 450
BveI ACCTGC 2 cut(s) 317, 632
Cac8I GCNNGC 2 cut(s) 251, 255
CfoI GCGC 1 cut(s) 593
Cfr13I GGNCC 1 cut(s) 781
ClaI ATCGAT 1 cut(s) 47
CspCI CAANNNNNGTGG 2 cut(s) 406, 441
CviAII CATG 6 cut(s) 52, 182, 254, 374, 420, 758
DdeI CTNAG 3 cut(s) 29, 315, 410
DpnI GATC 5 cut(s) 16, 50, 84, 99, 447
DpnII GATC 5 cut(s) 14, 48, 82, 97, 445
EaeI YGGCCR 1 cut(s) 76
Eam1104I CTCTTC 1 cut(s) 498
EarI CTCTTC 1 cut(s) 498
Eco130I CCWWGG 2 cut(s) 93, 181
Eco147I AGGCCT 1 cut(s) 613
Eco47III AGCGCT 1 cut(s) 592
Eco57I CTGAAG 4 cut(s) 42, 206, 498, 514
EcoRII CCWGG 2 cut(s) 126, 633
EcoT14I CCWWGG 2 cut(s) 93, 181
EcoT22I ATGCAT 1 cut(s) 466
ErhI CCWWGG 2 cut(s) 93, 181
Esp3I CGTCTC 1 cut(s) 694
FaeI CATG 6 cut(s) 55, 185, 257, 377, 423, 761
FatI CATG 6 cut(s) 51, 181, 253, 373, 419, 757
FauNDI CATATG 1 cut(s) 466
FblI GTMKAC 2 cut(s) 72, 403
Fnu4HI GCNGC 6 cut(s) 367, 732, 744, 747, 750, 764
FokI GGATG 5 cut(s) 97, 221, 312, 512, 521
Fsp4HI GCNGC 6 cut(s) 367, 732, 744, 747, 750, 764
FspBI CTAG 4 cut(s) 434, 515, 648, 666
GlaI GCGC 1 cut(s) 592
GluI GCNGC 6 cut(s) 367, 732, 744, 747, 750, 764
HaeII RGCGCY 1 cut(s) 594
HaeIII GGCC 3 cut(s) 78, 613, 783
HapII CCGG 1 cut(s) 784
HhaI GCGC 1 cut(s) 593
Hin1II CATG 6 cut(s) 55, 185, 257, 377, 423, 761
Hin6I GCGC 1 cut(s) 591
HinP1I GCGC 1 cut(s) 591
HincII GTYRAC 1 cut(s) 73
HindII GTYRAC 1 cut(s) 73
HindIII AAGCTT 1 cut(s) 522
HinfI GANTC 2 cut(s) 4, 473
HpaII CCGG 1 cut(s) 784
HphI GGTGA 3 cut(s) 77, 294, 298
Hpy166II GTNNAC 5 cut(s) 73, 323, 404, 619, 662
Hpy188I TCNGA 1 cut(s) 478
Hpy188III TCNNGA 4 cut(s) 224, 340, 449, 659
Hpy8I GTNNAC 5 cut(s) 73, 323, 404, 619, 662
Hpy99I CGWCG 2 cut(s) 74, 77
HpyAV CCTTC 1 cut(s) 558
HpyCH4III ACNGT 1 cut(s) 263
HpyCH4IV ACGT 1 cut(s) 69
HpyCH4V TGCA 7 cut(s) 105, 197, 266, 273, 464, 749, 766
HpyF10VI GCNNNNNNNGC 4 cut(s) 263, 713, 740, 749
HpyF3I CTNAG 3 cut(s) 29, 315, 410
HpySE526I ACGT 1 cut(s) 69
Hsp92II CATG 6 cut(s) 55, 185, 257, 377, 423, 761
HspAI GCGC 1 cut(s) 591
Kzo9I GATC 5 cut(s) 14, 48, 82, 97, 445
Lsp1109I GCAGC 6 cut(s) 378, 718, 730, 733, 750, 761
MaeI CTAG 4 cut(s) 434, 515, 648, 666
MaeII ACGT 1 cut(s) 69
MaeIII GTNAC 2 cut(s) 286, 354
MalI GATC 5 cut(s) 16, 50, 84, 99, 447
MboI GATC 5 cut(s) 14, 48, 82, 97, 445
MboII GAAGA 6 cut(s) 161, 269, 464, 491, 515, 645
MflI RGATCY 1 cut(s) 97
MhlI GDGCHC 1 cut(s) 718
MluCI AATT 2 cut(s) 106, 154
MnlI CCTC 9 cut(s) 28, 324, 382, 436, 441, 490, 499, 670, 705
Mph1103I ATGCAT 1 cut(s) 466
MseI TTAA 1 cut(s) 720
MspCI CTTAAG 1 cut(s) 719
MspI CCGG 1 cut(s) 784
MspR9I CCNGG 2 cut(s) 128, 635
Mva1269I GAATGC 1 cut(s) 273
MvaI CCWGG 2 cut(s) 128, 635
MvnI CGCG 1 cut(s) 450
MwoI GCNNNNNNNGC 4 cut(s) 263, 713, 740, 749
NcoI CCATGG 1 cut(s) 181
NdeI CATATG 1 cut(s) 466
NdeII GATC 5 cut(s) 14, 48, 82, 97, 445
NlaIII CATG 6 cut(s) 55, 185, 257, 377, 423, 761
NlaIV GGNNCC 1 cut(s) 179
NmuCI GTSAC 2 cut(s) 286, 354
NruI TCGCGA 1 cut(s) 450
NsiI ATGCAT 1 cut(s) 466
NspI RCATGY 1 cut(s) 257
PaeI GCATGC 1 cut(s) 257
PaqCI CACCTGC 2 cut(s) 317, 632
PceI AGGCCT 1 cut(s) 613
PcsI WCGNNNNNNNCGW 1 cut(s) 78
PctI GAATGC 1 cut(s) 273
PfeI GAWTC 2 cut(s) 4, 473
PfoI TCCNGGA 1 cut(s) 633
PkrI GCNGC 6 cut(s) 368, 733, 745, 748, 751, 765
Psp6I CCWGG 2 cut(s) 126, 633
PspGI CCWGG 2 cut(s) 126, 633
PspN4I GGNNCC 1 cut(s) 179
PspPI GGNCC 1 cut(s) 781
PstI CTGCAG 1 cut(s) 751
PsuI RGATCY 1 cut(s) 97
RruI TCGCGA 1 cut(s) 450
SalI GTCGAC 1 cut(s) 71
SaqAI TTAA 1 cut(s) 720
SatI GCNGC 6 cut(s) 367, 732, 744, 747, 750, 764
Sau3AI GATC 5 cut(s) 14, 48, 82, 97, 445
Sau96I GGNCC 1 cut(s) 781
ScrFI CCNGG 2 cut(s) 128, 635
SduI GDGCHC 1 cut(s) 718
SfcI CTRYAG 1 cut(s) 747
SgrDI CGTCGACG 1 cut(s) 71
SmlI CTYRAG 1 cut(s) 719
SmoI CTYRAG 1 cut(s) 719
SphI GCATGC 1 cut(s) 257
Sse9I AATT 2 cut(s) 106, 154
SseBI AGGCCT 1 cut(s) 613
SsiI CCGC 1 cut(s) 734
SspMI CTAG 4 cut(s) 434, 515, 648, 666
StuI AGGCCT 1 cut(s) 613
StyD4I CCNGG 2 cut(s) 126, 633
StyI CCWWGG 2 cut(s) 93, 181
TaaI ACNGT 1 cut(s) 263
TaiI ACGT 1 cut(s) 72
TaqI TCGA 5 cut(s) 47, 72, 333, 387, 562
TaqII GACCGA 1 cut(s) 342
TasI AATT 2 cut(s) 106, 154
TfiI GAWTC 2 cut(s) 4, 473
Tru1I TTAA 1 cut(s) 720
Tru9I TTAA 1 cut(s) 720
TscAI CASTG 1 cut(s) 323
TseFI GTSAC 2 cut(s) 286, 354
TseI GCWGC 6 cut(s) 366, 731, 743, 746, 749, 763
Tsp45I GTSAC 2 cut(s) 286, 354
TspDTI ATGAA 4 cut(s) 99, 516, 528, 534
TspGWI ACGGA 1 cut(s) 539
TspRI CASTG 1 cut(s) 323
Vha464I CTTAAG 1 cut(s) 719
XceI RCATGY 1 cut(s) 257
XmiI GTMKAC 2 cut(s) 72, 403
XspI CTAG 4 cut(s) 434, 515, 648, 666
Zsp2I ATGCAT 1 cut(s) 466
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.