Rorug05G0262200

Zinc finger, C3HC4 type (RING finger)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0007205
Physical Location & Seq
Forward (+)
29674816 .. 29692843
18028 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0262200.1

Sequence Viewer

Length: 1644 bp
ATGGCGTCCATTCTTCCAGCTCACTCTGCTTCCTCAAAATCTGAGACCGGTGATCTTCACGTCCGTCGTGAAGAAAAACTGAGGGAGTTGAAGGAAACACTCGCAAATGCCGGAGAAGATCAACTGCAGCAATTGGTTATGGTTGATGCCATGCAATGCCTCGGCGTCGCGTATCTCTTCCAAAATGAAATTTATGCAATCTTAGAGAAGCAATACAACAGGTCATCAGCTTATAATGGTGATGACGATGGCAATCATCAACTTCTTGAGGCTTCCCTTCGTTTTCGACTACTGAGACAACAAGGTTTCCCTGTAACTCCTGAGGCGTGTAACAAGATCAAGAACAATGAGAAGCTCAAATCCGGACTAATAGCTAGTGATCATGAAGACATAAAAGGATTAGTGGAATTATGTGAAGCATCTCACTTGAGTTTCCAAGGAGAAGAAATGCTTGACGAAGCAGGAAAGCTCAGCCGCCAGCTTCTCACTGCCTGGGTCTCTAACAATCACAATGATCATCGCGTAACACTTGTTGAGCGCACTTTGGAGCATCCCTATCACAAGAGCCTGACAAGATTCATGGCCAAGAAGTTTTTGAATAATTTTCAAGGCCAAGAAAAATGGGTTTGTTCTTTACAAGAGCTAGCAAACCTAGAGTTCAACATGGTTGAATCCATAATAAAAAATGAAATCCTACTAGTATCTGGATGGTGGAAAGAACTAGGTTTGACCAAAGAGTTGAAGTTTGTGAGAGACCAGCCAATTAAATGGTACATATGGCCAATGGCATGCCTCGTTGATCCACGATTATCAGAGGAAATGATTGAACTCACTAAAGCAGTCTCTCTTGTTTACATTATAGATGATATTTTTGATGTCCGTGGAACACTAGATGAACTCGTTCTCTTTACAGCAGCAGTTGTGAGATGGGATACTACTACAAATGATGAGCTACCGAATTACATGAAAACATGTTTCAAATCTCTTAATGACATTACCAATAATATCAGCCAGAGTGTCTACGAAAAGCACGGATGGAACCCTATTCAAACCCTAAGAGAATCGTGGATAAGATTGTGTAAGGCATTCTTAGTAGAAGCACAATGGTTTAGGCATGGGGAGTTGCCAAACTCCGGAGACTACTTGAAGAATGGGATTATCAGTTCAGGGGTGCCTGCACTTTTAACCCACGCCTTCTTTATTCTTGGTCAAGGTATGACCAAGGAAACCATAGATCTTTTCAACAACATCAACTTACCTAGTATCGTGTCTTCAATGGCCACGATACTTCGGCTATGGGATGACTTTGGAACTGCCAAGGATGAGGACCAAAATGGGTTTGATGGATCATACATAGAGTGCTACATGAAAGAACACAAAGGCTCTTCTGTTGAAGAGGTGCAAGCATATGTAATCCAAAAGATTTCAGATGAGTGGAAGTGCCTCAACCAAGAATGCTTCTCTGCTTGCAATCCATTTTCAGAGTTCTTTACACAATTCGCTCTCAATATTTCTAGAATGGTTCCCATGATGTACGATTACAATGCCCATCATTGTCTTCCAAGCCTTGAGGAGAATATGAAGTCGTTGCTTTCGGATAGTTTTCTTGCCAAAGGCAGCATCTACGGGAAATCCATGCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4 zf-RING_2

Protein Analysis

547

Amino Acids

62.66

Weight (kDa)

5.23

Isoelectric Point (pI)

42.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Terpene_synth PF01397 15 - 177 7.6e-32 Terpene synthase, N-terminal domain
Terpene_synth_C PF03936 239 - 480 7.1e-74 Terpene synthase family, metal binding domain
Terpene_syn_C_2 PF19086 279 - 480 5.2e-47 Terpene synthase family 2, C-terminal metal binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 234
AccB1I GGYRCC 1 cut(s) 1171
AccI GTMKAC 1 cut(s) 1020
AccII CGCG 2 cut(s) 170, 522
AccIII TCCGGA 2 cut(s) 362, 1133
AciI CCGC 1 cut(s) 475
AclWI GGATC 2 cut(s) 794, 1354
AcoI YGGCCR 3 cut(s) 582, 779, 1278
AcsI RAATTY 1 cut(s) 189
AcyI GRCGYC 2 cut(s) 5, 165
AfaI GTAC 2 cut(s) 773, 1535
AfiI CCNNNNNNNGG 1 cut(s) 1133
AflIII ACRYGT 1 cut(s) 971
AgeI ACCGGT 1 cut(s) 47
AhlI ACTAGT 1 cut(s) 697
AjiI CACGTC 1 cut(s) 61
AjnI CCWGG 1 cut(s) 491
AloI GAACNNNNNNTCC 2 cut(s) 1147, 1179
AluBI AGCT 8 cut(s) 20, 230, 355, 374, 469, 481, 643, 952
AluI AGCT 8 cut(s) 20, 230, 355, 374, 469, 481, 643, 952
Alw26I GTCTC 6 cut(s) 38, 289, 502, 747, 847, 1131
AlwI GGATC 2 cut(s) 794, 1354
Aor13HI TCCGGA 2 cut(s) 362, 1133
AoxI GGCC 4 cut(s) 582, 610, 779, 1278
ApeKI GCWGC 3 cut(s) 127, 914, 1617
ApoI RAATTY 1 cut(s) 189
AsiGI ACCGGT 1 cut(s) 47
Asp700I GAANNNNTTC 1 cut(s) 900
AspLEI GCGC 1 cut(s) 540
AspS9I GGNCC 1 cut(s) 1327
AsuHPI GGTGA 2 cut(s) 62, 251
AsuNHI GCTAGC 1 cut(s) 643
AvaII GGWCC 1 cut(s) 1327
AxyI CCTNAGG 1 cut(s) 321
BalI TGGCCA 3 cut(s) 584, 781, 1280
BanI GGYRCC 1 cut(s) 1171
BarI GAAGNNNNNNTAC 2 cut(s) 1255, 1287
BbsI GAAGAC 3 cut(s) 393, 1263, 1550
BbvI GCAGC 3 cut(s) 139, 926, 1629
BccI CCATC 6 cut(s) 242, 702, 921, 1029, 1337, 1557
BcgI CGANNNNNNTGC 2 cut(s) 1526, 1560
BciT130I CCWGG 1 cut(s) 493
BciVI GTATCC 1 cut(s) 925
BclI TGATCA 2 cut(s) 379, 514
BcoDI GTCTC 6 cut(s) 38, 289, 502, 747, 847, 1131
BcuI ACTAGT 1 cut(s) 697
BfaI CTAG 8 cut(s) 375, 644, 653, 698, 722, 890, 1260, 1515
BfmI CTRYAG 1 cut(s) 125
BfuI GTATCC 1 cut(s) 925
BglII AGATCT 1 cut(s) 1234
BisI GCNGC 4 cut(s) 128, 475, 915, 1618
BlpI GCTNAGC 1 cut(s) 470
BlsI GCNGC 4 cut(s) 129, 476, 916, 1619
Bme1390I CCNGG 1 cut(s) 493
Bme18I GGWCC 1 cut(s) 1327
BmgBI CACGTC 1 cut(s) 61
BmgT120I GGNCC 1 cut(s) 1327
BmiI GGNNCC 3 cut(s) 1040, 1173, 1524
BmrFI CCNGG 1 cut(s) 493
BmsI GCATC 4 cut(s) 136, 428, 559, 1629
BmtI GCTAGC 1 cut(s) 647
BpiI GAAGAC 3 cut(s) 393, 1263, 1550
Bpu1102I GCTNAGC 1 cut(s) 470
BpuEI CTTGAG 3 cut(s) 287, 448, 1589
BsaBI GATNNNNATC 1 cut(s) 252
BsaHI GRCGYC 2 cut(s) 5, 165
BsaI GGTCTC 3 cut(s) 38, 502, 747
BsaJI CCNNGG 6 cut(s) 160, 436, 492, 880, 1221, 1317
BsaWI WCCGGW 3 cut(s) 47, 362, 1133
Bsc4I CCNNNNNNNGG 1 cut(s) 1133
Bse118I RCCGGY 1 cut(s) 47
Bse21I CCTNAGG 1 cut(s) 321
Bse3DI GCAATG 1 cut(s) 161
Bse8I GATNNNNATC 1 cut(s) 252
BseAI TCCGGA 2 cut(s) 362, 1133
BseBI CCWGG 1 cut(s) 493
BseDI CCNNGG 6 cut(s) 160, 436, 492, 880, 1221, 1317
BseGI GGATG 5 cut(s) 550, 713, 1040, 1306, 1327
BseJI GATNNNNATC 1 cut(s) 252
BseLI CCNNNNNNNGG 1 cut(s) 1133
BseMI GCAATG 1 cut(s) 161
BseMII CTCAG 5 cut(s) 33, 71, 284, 312, 484
BseRI GAGGAG 1 cut(s) 1586
BseXI GCAGC 3 cut(s) 139, 926, 1629
BsgI GTGCAG 1 cut(s) 1161
Bsh1236I CGCG 2 cut(s) 170, 522
BshFI GGCC 4 cut(s) 584, 612, 781, 1280
BshNI GGYRCC 1 cut(s) 1171
BshTI ACCGGT 1 cut(s) 47
BsiSI CCGG 4 cut(s) 48, 111, 363, 1134
BslI CCNNNNNNNGG 1 cut(s) 1133
BsmAI GTCTC 6 cut(s) 38, 289, 502, 747, 847, 1131
BsmI GAATGC 2 cut(s) 1085, 1460
BsnI GGCC 4 cut(s) 584, 612, 781, 1280
Bso31I GGTCTC 3 cut(s) 38, 502, 747
Bsp13I TCCGGA 2 cut(s) 362, 1133
Bsp143I GATC 8 cut(s) 52, 118, 336, 379, 514, 799, 1234, 1346
Bsp1720I GCTNAGC 1 cut(s) 470
BspACI CCGC 1 cut(s) 475
BspANI GGCC 4 cut(s) 584, 612, 781, 1280
BspCNI CTCAG 5 cut(s) 34, 72, 285, 313, 483
BspEI TCCGGA 2 cut(s) 362, 1133
BspFNI CGCG 2 cut(s) 170, 522
BspHI TCATGA 1 cut(s) 382
BspLI GGNNCC 3 cut(s) 1040, 1173, 1524
BspMAI CTGCAG 1 cut(s) 129
BspOI GCTAGC 1 cut(s) 647
BspPI GGATC 2 cut(s) 794, 1354
BspQI GCTCTTC 1 cut(s) 1390
BspT107I GGYRCC 1 cut(s) 1171
BspTNI GGTCTC 3 cut(s) 38, 502, 747
BsrDI GCAATG 1 cut(s) 161
BsrFI RCCGGY 1 cut(s) 47
BssAI RCCGGY 1 cut(s) 47
BssECI CCNNGG 6 cut(s) 160, 436, 492, 880, 1221, 1317
BssMI GATC 8 cut(s) 52, 118, 336, 379, 514, 799, 1234, 1346
BssNI GRCGYC 2 cut(s) 5, 165
BssT1I CCWWGG 3 cut(s) 436, 1221, 1317
Bst2UI CCWGG 1 cut(s) 493
Bst6I CTCTTC 3 cut(s) 182, 1389, 1390
BstACI GRCGYC 2 cut(s) 5, 165
BstC8I GCNNGC 6 cut(s) 479, 645, 790, 1176, 1404, 1468
BstDEI CTNAG 8 cut(s) 42, 80, 202, 293, 321, 470, 1055, 1090
BstDSI CCRYGG 1 cut(s) 880
BstF5I GGATG 5 cut(s) 550, 713, 1040, 1306, 1327
BstFNI CGCG 2 cut(s) 170, 522
BstHHI GCGC 1 cut(s) 540
BstKTI GATC 8 cut(s) 55, 121, 339, 382, 517, 802, 1237, 1349
BstMAI GTCTC 6 cut(s) 38, 289, 502, 747, 847, 1131
BstMBI GATC 8 cut(s) 52, 118, 336, 379, 514, 799, 1234, 1346
BstMWI GCNNNNNNNGC 1 cut(s) 26
BstNI CCWGG 1 cut(s) 493
BstNSI RCATGY 2 cut(s) 792, 975
BstSCI CCNGG 1 cut(s) 491
BstSFI CTRYAG 1 cut(s) 125
BstUI CGCG 2 cut(s) 170, 522
BstV1I GCAGC 3 cut(s) 139, 926, 1629
BstV2I GAAGAC 3 cut(s) 393, 1263, 1550
BstX2I RGATCY 1 cut(s) 1234
BstXI CCANNNNNNTGG 1 cut(s) 768
BstYI RGATCY 1 cut(s) 1234
Bsu36I CCTNAGG 1 cut(s) 321
BsuI GTATCC 1 cut(s) 925
BsuRI GGCC 4 cut(s) 584, 612, 781, 1280
BtgI CCRYGG 1 cut(s) 880
BtgZI GCGATG 1 cut(s) 503
BtrI CACGTC 1 cut(s) 61
BtsCI GGATG 5 cut(s) 550, 713, 1040, 1306, 1327
BtsI GCAGTG 1 cut(s) 486
BtsIMutI CAGTG 1 cut(s) 486
Cac8I GCNNGC 6 cut(s) 479, 645, 790, 1176, 1404, 1468
CciI TCATGA 1 cut(s) 382
CfoI GCGC 1 cut(s) 540
Cfr10I RCCGGY 1 cut(s) 47
Cfr13I GGNCC 1 cut(s) 1327
CseI GACGC 1 cut(s) 154
Csp6I GTAC 2 cut(s) 772, 1534
CspAI ACCGGT 1 cut(s) 47
CviQI GTAC 2 cut(s) 772, 1534
DdeI CTNAG 8 cut(s) 42, 80, 202, 293, 321, 470, 1055, 1090
DpnI GATC 8 cut(s) 54, 120, 338, 381, 516, 801, 1236, 1348
DpnII GATC 8 cut(s) 52, 118, 336, 379, 514, 799, 1234, 1346
EaeI YGGCCR 3 cut(s) 582, 779, 1278
Eam1104I CTCTTC 3 cut(s) 182, 1389, 1390
EarI CTCTTC 3 cut(s) 182, 1389, 1390
Eco130I CCWWGG 3 cut(s) 436, 1221, 1317
Eco31I GGTCTC 3 cut(s) 38, 502, 747
Eco47I GGWCC 1 cut(s) 1327
Eco81I CCTNAGG 1 cut(s) 321
EcoRII CCWGG 1 cut(s) 491
EcoT14I CCWWGG 3 cut(s) 436, 1221, 1317
ErhI CCWWGG 3 cut(s) 436, 1221, 1317
FalI AAGNNNNNCTT 4 cut(s) 435, 467, 1073, 1105
FauNDI CATATG 2 cut(s) 776, 1408
FbaI TGATCA 2 cut(s) 379, 514
FblI GTMKAC 1 cut(s) 1020
Fnu4HI GCNGC 4 cut(s) 128, 475, 915, 1618
FokI GGATG 5 cut(s) 537, 720, 1047, 1313, 1334
Fsp4HI GCNGC 4 cut(s) 128, 475, 915, 1618
FspBI CTAG 8 cut(s) 375, 644, 653, 698, 722, 890, 1260, 1515
GlaI GCGC 1 cut(s) 539
GluI GCNGC 4 cut(s) 128, 475, 915, 1618
HaeIII GGCC 4 cut(s) 584, 612, 781, 1280
HapII CCGG 4 cut(s) 48, 111, 363, 1134
HgaI GACGC 1 cut(s) 154
HhaI GCGC 1 cut(s) 540
Hin1I GRCGYC 2 cut(s) 5, 165
Hin6I GCGC 1 cut(s) 538
HinP1I GCGC 1 cut(s) 538
HinfI GANTC 3 cut(s) 576, 671, 1061
HpaII CCGG 4 cut(s) 48, 111, 363, 1134
HphI GGTGA 2 cut(s) 62, 251
Hpy166II GTNNAC 2 cut(s) 853, 1021
Hpy188I TCNGA 5 cut(s) 43, 814, 1429, 1483, 1597
Hpy188III TCNNGA 9 cut(s) 68, 266, 320, 340, 363, 383, 705, 1134, 1515
Hpy8I GTNNAC 2 cut(s) 853, 1021
Hpy99I CGWCG 2 cut(s) 69, 170
HpyAV CCTTC 3 cut(s) 85, 287, 1204
HpyCH4IV ACGT 1 cut(s) 60
HpyCH4V TGCA 7 cut(s) 127, 154, 197, 1178, 1402, 1470, 1639
HpyF10VI GCNNNNNNNGC 1 cut(s) 26
HpyF3I CTNAG 8 cut(s) 42, 80, 202, 293, 321, 470, 1055, 1090
HpySE526I ACGT 1 cut(s) 60
Hsp92I GRCGYC 2 cut(s) 5, 165
HspAI GCGC 1 cut(s) 538
Kpn2I TCCGGA 2 cut(s) 362, 1133
Ksp22I TGATCA 2 cut(s) 379, 514
Kzo9I GATC 8 cut(s) 52, 118, 336, 379, 514, 799, 1234, 1346
LguI GCTCTTC 1 cut(s) 1390
LmnI GCTCC 1 cut(s) 547
Lsp1109I GCAGC 3 cut(s) 139, 926, 1629
LweI GCATC 4 cut(s) 136, 428, 559, 1629
MaeI CTAG 8 cut(s) 375, 644, 653, 698, 722, 890, 1260, 1515
MaeII ACGT 1 cut(s) 60
MaeIII GTNAC 3 cut(s) 313, 329, 523
MalI GATC 8 cut(s) 54, 120, 338, 381, 516, 801, 1236, 1348
MboI GATC 8 cut(s) 52, 118, 336, 379, 514, 799, 1234, 1346
MfeI CAATTG 1 cut(s) 131
MflI RGATCY 1 cut(s) 1234
MlsI TGGCCA 3 cut(s) 584, 781, 1280
MluCI AATT 7 cut(s) 131, 189, 407, 601, 762, 958, 1496
MluNI TGGCCA 3 cut(s) 584, 781, 1280
Mox20I TGGCCA 3 cut(s) 584, 781, 1280
MroI TCCGGA 2 cut(s) 362, 1133
MroXI GAANNNNTTC 1 cut(s) 900
MscI TGGCCA 3 cut(s) 584, 781, 1280
MseI TTAA 3 cut(s) 765, 987, 1184
Msp20I TGGCCA 3 cut(s) 584, 781, 1280
MspI CCGG 4 cut(s) 48, 111, 363, 1134
MspR9I CCNGG 1 cut(s) 493
MunI CAATTG 1 cut(s) 131
Mva1269I GAATGC 2 cut(s) 1085, 1460
MvaI CCWGG 1 cut(s) 493
MvnI CGCG 2 cut(s) 170, 522
MwoI GCNNNNNNNGC 1 cut(s) 26
NdeI CATATG 2 cut(s) 776, 1408
NdeII GATC 8 cut(s) 52, 118, 336, 379, 514, 799, 1234, 1346
NheI GCTAGC 1 cut(s) 643
NlaIV GGNNCC 3 cut(s) 1040, 1173, 1524
NmeAIII GCCGAG 1 cut(s) 141
NspI RCATGY 2 cut(s) 792, 975
PaeI GCATGC 1 cut(s) 792
PagI TCATGA 1 cut(s) 382
PciI ACATGT 1 cut(s) 971
PciSI GCTCTTC 1 cut(s) 1390
PctI GAATGC 2 cut(s) 1085, 1460
PdmI GAANNNNTTC 1 cut(s) 900
PfeI GAWTC 3 cut(s) 576, 671, 1061
PinAI ACCGGT 1 cut(s) 47
PkrI GCNGC 4 cut(s) 129, 476, 916, 1619
PscI ACATGT 1 cut(s) 971
PsiI TTATAA 1 cut(s) 234
Psp6I CCWGG 1 cut(s) 491
PspGI CCWGG 1 cut(s) 491
PspN4I GGNNCC 3 cut(s) 1040, 1173, 1524
PspPI GGNCC 1 cut(s) 1327
PstI CTGCAG 1 cut(s) 129
PsuI RGATCY 1 cut(s) 1234
RsaI GTAC 2 cut(s) 773, 1535
RsaNI GTAC 2 cut(s) 772, 1534
SapI GCTCTTC 1 cut(s) 1390
SaqAI TTAA 3 cut(s) 765, 987, 1184
SatI GCNGC 4 cut(s) 128, 475, 915, 1618
Sau3AI GATC 8 cut(s) 52, 118, 336, 379, 514, 799, 1234, 1346
Sau96I GGNCC 1 cut(s) 1327
ScrFI CCNGG 1 cut(s) 493
SfaNI GCATC 4 cut(s) 136, 428, 559, 1629
SfcI CTRYAG 1 cut(s) 125
SinI GGWCC 1 cut(s) 1327
SmlI CTYRAG 3 cut(s) 266, 427, 1568
SmoI CTYRAG 3 cut(s) 266, 427, 1568
SpeI ACTAGT 1 cut(s) 697
SphI GCATGC 1 cut(s) 792
Sse9I AATT 7 cut(s) 131, 189, 407, 601, 762, 958, 1496
SsiI CCGC 1 cut(s) 475
SspI AATATT 1 cut(s) 1510
SspMI CTAG 8 cut(s) 375, 644, 653, 698, 722, 890, 1260, 1515
StyD4I CCNGG 1 cut(s) 491
StyI CCWWGG 3 cut(s) 436, 1221, 1317
TaiI ACGT 1 cut(s) 63
TaqI TCGA 1 cut(s) 286
TasI AATT 7 cut(s) 131, 189, 407, 601, 762, 958, 1496
TauI GCSGC 1 cut(s) 477
TfiI GAWTC 3 cut(s) 576, 671, 1061
Tru1I TTAA 3 cut(s) 765, 987, 1184
Tru9I TTAA 3 cut(s) 765, 987, 1184
TscAI CASTG 1 cut(s) 493
TseI GCWGC 3 cut(s) 127, 914, 1617
TspDTI ATGAA 8 cut(s) 201, 399, 568, 702, 909, 980, 1382, 1595
TspGWI ACGGA 3 cut(s) 53, 869, 1047
TspRI CASTG 1 cut(s) 493
VpaK11BI GGWCC 1 cut(s) 1327
XapI RAATTY 1 cut(s) 189
XbaI TCTAGA 1 cut(s) 1514
XceI RCATGY 2 cut(s) 792, 975
XmiI GTMKAC 1 cut(s) 1020
XmnI GAANNNNTTC 1 cut(s) 900
XspI CTAG 8 cut(s) 375, 644, 653, 698, 722, 890, 1260, 1515
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.