AT5G20635

Guanine nucleotide-binding protein subunit gamma

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0007422
Physical Location & Seq
Reverse (-)
6979157 .. 6982163
3007 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G20635.2

Sequence Viewer

Length: 756 bp
ATGTCTGCTCCTTCTGGCGGTGGCGAAGGAGGAGGAAAAGAATCAGCTGCTGGTGGAGTGAGTTCATCGTCTCTTGCTCCGTCGTCTCTACCACCGCCTCGTCCTAAGTCTCCACCAGAGTATCCAGATTTGTACGGGAAACGCAGAGAGGCGGCGAGAGTTCAGATGCTCGAGAGAGAGATTGGTTTTCTCGAGGGCGAAATTAAATTCATCGAAGGCGTACAACCGGCATCTAGATGCATCAAAGAAGTCTCTGATTTTGTTGTTGCAAATTCTGACCCATTGATCCCTGCACAACGAAAAAGTCGAAGATCCTTCCGGTTCTGGAAGTGGCTCTGTGGCCCATGTTTGAGCCTGGTGAGTTTCTGCTGTTGCTGCCAATCCAAATGTTCGTGCCATCTGAGGAAACCCAAGTGCTGCAACTGTACATCTTGCAGCTGTATAGGGTCCAAATGCTGTGACGGGTCATGCTGCTCAAACATTTGTTGTTGCCCGAGACTAAGCTGCCCGAGCTGTTCATGCTTCCGAGGTTGCTGGTGTTCTTGTCCGGACATGTCTTGCTGCATTCCCAGCTGTTTCCGCAGTTGCAGTTGCACTCGACCGTCGTGTCTGAATAAAAAGAAGAGCTCATGCTGCAGCTGCAACTGCAAGATCAGATGGTCATCTTGTTTTAGTTGTCCCAAGGTACGACTTTGTTCTTGTTGTTTTTGCAATTGTAAAAATCTATGTTCTAATCCTTGTTGTTTAGCTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0005575 GO:0005623 GO:0005886 GO:0008150 GO:0009719 GO:0009725 GO:0009737 GO:0010033 GO:0016020 GO:0033993 GO:0042221 GO:0044464 GO:0050896 GO:0071944 GO:0097305 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
G-gamma

Protein Analysis

251

Amino Acids

27.16

Weight (kDa)

8.75

Isoelectric Point (pI)

86.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 606
AccIII TCCGGA 1 cut(s) 547
AciI CCGC 4 cut(s) 18, 95, 152, 580
AclWI GGATC 2 cut(s) 280, 306
AcsI RAATTY 2 cut(s) 206, 271
AfaI GTAC 4 cut(s) 134, 222, 427, 687
AfiI CCNNNNNNNGG 1 cut(s) 17
AflIII ACRYGT 1 cut(s) 552
AjnI CCWGG 1 cut(s) 354
AluBI AGCT 8 cut(s) 47, 438, 504, 513, 573, 627, 639, 749
AluI AGCT 8 cut(s) 47, 438, 504, 513, 573, 627, 639, 749
Alw21I GWGCWC 1 cut(s) 629
Alw26I GTCTC 5 cut(s) 75, 90, 114, 256, 490
AlwI GGATC 2 cut(s) 280, 306
AlwNI CAGNNNCTG 1 cut(s) 50
Ama87I CYCGRG 4 cut(s) 170, 191, 493, 508
Aor13HI TCCGGA 1 cut(s) 547
AoxI GGCC 1 cut(s) 340
ApoI RAATTY 2 cut(s) 206, 271
AspS9I GGNCC 2 cut(s) 341, 447
AsuHPI GGTGA 1 cut(s) 370
AvaI CYCGRG 4 cut(s) 170, 191, 493, 508
AvaII GGWCC 1 cut(s) 447
BaeI ACNNNNGTAYC 2 cut(s) 104, 137
BanII GRGCYC 1 cut(s) 629
Bbv12I GWGCWC 1 cut(s) 629
BccI CCATC 2 cut(s) 405, 651
BciT130I CCWGG 1 cut(s) 356
BciVI GTATCC 1 cut(s) 132
BcoDI GTCTC 5 cut(s) 75, 90, 114, 256, 490
BfaI CTAG 1 cut(s) 234
BfmI CTRYAG 1 cut(s) 634
BfuI GTATCC 1 cut(s) 132
Bme1390I CCNGG 1 cut(s) 356
Bme18I GGWCC 1 cut(s) 447
BmeT110I CYCGRG 4 cut(s) 170, 191, 493, 508
BmgT120I GGNCC 2 cut(s) 341, 447
BmiI GGNNCC 1 cut(s) 448
BmrFI CCNGG 1 cut(s) 356
BmsI GCATC 4 cut(s) 156, 227, 239, 249
BsaBI GATNNNNATC 1 cut(s) 661
BsaJI CCNNGG 2 cut(s) 526, 681
BsaWI WCCGGW 2 cut(s) 318, 547
Bsc4I CCNNNNNNNGG 1 cut(s) 17
Bse118I RCCGGY 1 cut(s) 226
Bse8I GATNNNNATC 1 cut(s) 661
BseAI TCCGGA 1 cut(s) 547
BseBI CCWGG 1 cut(s) 356
BseDI CCNNGG 2 cut(s) 526, 681
BseJI GATNNNNATC 1 cut(s) 661
BseLI CCNNNNNNNGG 1 cut(s) 17
BseMII CTCAG 1 cut(s) 392
BseRI GAGGAG 1 cut(s) 45
BseYI CCCAGC 1 cut(s) 569
BsgI GTGCAG 1 cut(s) 276
Bsh1285I CGRYCG 1 cut(s) 602
BshFI GGCC 1 cut(s) 342
BsiEI CGRYCG 1 cut(s) 602
BsiHKAI GWGCWC 1 cut(s) 629
BsiHKCI CYCGRG 4 cut(s) 170, 191, 493, 508
BsiSI CCGG 3 cut(s) 227, 319, 548
BslFI GGGAC 1 cut(s) 663
BslI CCNNNNNNNGG 1 cut(s) 17
BsmAI GTCTC 5 cut(s) 75, 90, 114, 256, 490
BsmBI CGTCTC 2 cut(s) 75, 90
BsmFI GGGAC 1 cut(s) 663
BsmI GAATGC 1 cut(s) 564
BsnI GGCC 1 cut(s) 342
BsoBI CYCGRG 4 cut(s) 170, 191, 493, 508
Bsp1286I GDGCHC 1 cut(s) 629
Bsp13I TCCGGA 1 cut(s) 547
Bsp1407I TGTACA 1 cut(s) 425
Bsp143I GATC 3 cut(s) 285, 311, 651
BspACI CCGC 4 cut(s) 18, 95, 152, 580
BspANI GGCC 1 cut(s) 342
BspCNI CTCAG 1 cut(s) 393
BspEI TCCGGA 1 cut(s) 547
BspLI GGNNCC 1 cut(s) 448
BspMAI CTGCAG 1 cut(s) 638
BspPI GGATC 2 cut(s) 280, 306
BspQI GCTCTTC 1 cut(s) 617
BsrFI RCCGGY 1 cut(s) 226
BsrGI TGTACA 1 cut(s) 425
BssAI RCCGGY 1 cut(s) 226
BssECI CCNNGG 2 cut(s) 526, 681
BssMI GATC 3 cut(s) 285, 311, 651
BssT1I CCWWGG 1 cut(s) 681
Bst2UI CCWGG 1 cut(s) 356
Bst4CI ACNGT 2 cut(s) 425, 603
Bst6I CTCTTC 1 cut(s) 617
BstAUI TGTACA 1 cut(s) 425
BstDEI CTNAG 3 cut(s) 105, 401, 500
BstKTI GATC 3 cut(s) 288, 314, 654
BstMAI GTCTC 5 cut(s) 75, 90, 114, 256, 490
BstMBI GATC 3 cut(s) 285, 311, 651
BstMCI CGRYCG 1 cut(s) 602
BstMWI GCNNNNNNNGC 8 cut(s) 375, 510, 519, 570, 579, 633, 639, 645
BstNI CCWGG 1 cut(s) 356
BstNSI RCATGY 1 cut(s) 556
BstSCI CCNGG 1 cut(s) 354
BstSFI CTRYAG 1 cut(s) 634
BstX2I RGATCY 1 cut(s) 311
BstYI RGATCY 1 cut(s) 311
BsuI GTATCC 1 cut(s) 132
BsuRI GGCC 1 cut(s) 342
CaiI CAGNNNCTG 1 cut(s) 50
Cfr10I RCCGGY 1 cut(s) 226
Cfr13I GGNCC 2 cut(s) 341, 447
Csp6I GTAC 4 cut(s) 133, 221, 426, 686
CviAII CATG 5 cut(s) 345, 468, 519, 553, 630
CviQI GTAC 4 cut(s) 133, 221, 426, 686
DdeI CTNAG 3 cut(s) 105, 401, 500
DpnI GATC 3 cut(s) 287, 313, 653
DpnII GATC 3 cut(s) 285, 311, 651
DrdI GACNNNNNNGTC 1 cut(s) 606
DseDI GACNNNNNNGTC 1 cut(s) 606
Eam1104I CTCTTC 1 cut(s) 617
EarI CTCTTC 1 cut(s) 617
Ecl136II GAGCTC 1 cut(s) 627
Eco130I CCWWGG 1 cut(s) 681
Eco24I GRGCYC 1 cut(s) 629
Eco47I GGWCC 1 cut(s) 447
Eco53kI GAGCTC 1 cut(s) 627
Eco88I CYCGRG 4 cut(s) 170, 191, 493, 508
EcoICRI GAGCTC 1 cut(s) 627
EcoRII CCWGG 1 cut(s) 354
EcoT14I CCWWGG 1 cut(s) 681
EcoT22I ATGCAT 1 cut(s) 242
EcoT38I GRGCYC 1 cut(s) 629
ErhI CCWWGG 1 cut(s) 681
Esp3I CGTCTC 2 cut(s) 75, 90
FaeI CATG 5 cut(s) 348, 471, 522, 556, 633
FaiI YATR 7 cut(s) 346, 443, 469, 520, 554, 631, 727
FaqI GGGAC 1 cut(s) 663
FatI CATG 5 cut(s) 344, 467, 518, 552, 629
FriOI GRGCYC 1 cut(s) 629
FspBI CTAG 1 cut(s) 234
GsaI CCCAGC 1 cut(s) 573
HaeIII GGCC 1 cut(s) 342
HapII CCGG 3 cut(s) 227, 319, 548
Hin1II CATG 5 cut(s) 348, 471, 522, 556, 633
HinfI GANTC 1 cut(s) 41
HpaII CCGG 3 cut(s) 227, 319, 548
HphI GGTGA 1 cut(s) 370
Hpy188I TCNGA 7 cut(s) 165, 256, 277, 402, 527, 612, 656
Hpy188III TCNNGA 6 cut(s) 125, 172, 191, 234, 325, 548
Hpy99I CGWCG 2 cut(s) 85, 607
HpyAV CCTTC 4 cut(s) 20, 21, 209, 325
HpyCH4III ACNGT 2 cut(s) 425, 603
HpyF10VI GCNNNNNNNGC 8 cut(s) 375, 510, 519, 570, 579, 633, 639, 645
HpyF3I CTNAG 3 cut(s) 105, 401, 500
Hsp92II CATG 5 cut(s) 348, 471, 522, 556, 633
Kpn2I TCCGGA 1 cut(s) 547
Kzo9I GATC 3 cut(s) 285, 311, 651
LguI GCTCTTC 1 cut(s) 617
LmnI GCTCC 2 cut(s) 13, 82
LweI GCATC 4 cut(s) 156, 227, 239, 249
MaeI CTAG 1 cut(s) 234
MaeIII GTNAC 1 cut(s) 458
MalI GATC 3 cut(s) 287, 313, 653
MboI GATC 3 cut(s) 285, 311, 651
MboII GAAGA 2 cut(s) 321, 634
MfeI CAATTG 1 cut(s) 712
MflI RGATCY 1 cut(s) 311
MhlI GDGCHC 1 cut(s) 629
MluCI AATT 4 cut(s) 201, 206, 271, 712
MnlI CCTC 7 cut(s) 23, 26, 108, 142, 187, 396, 521
Mph1103I ATGCAT 1 cut(s) 242
MroI TCCGGA 1 cut(s) 547
MseI TTAA 1 cut(s) 204
MslI CAYNNNNRTG 1 cut(s) 235
MspA1I CMGCKG 4 cut(s) 47, 438, 573, 639
MspI CCGG 3 cut(s) 227, 319, 548
MspR9I CCNGG 1 cut(s) 356
MunI CAATTG 1 cut(s) 712
Mva1269I GAATGC 1 cut(s) 564
MvaI CCWGG 1 cut(s) 356
MwoI GCNNNNNNNGC 8 cut(s) 375, 510, 519, 570, 579, 633, 639, 645
NdeII GATC 3 cut(s) 285, 311, 651
NlaIII CATG 5 cut(s) 348, 471, 522, 556, 633
NlaIV GGNNCC 1 cut(s) 448
NmuCI GTSAC 1 cut(s) 458
NsiI ATGCAT 1 cut(s) 242
NspI RCATGY 1 cut(s) 556
PaeR7I CTCGAG 2 cut(s) 170, 191
PciI ACATGT 1 cut(s) 552
PciSI GCTCTTC 1 cut(s) 617
PcsI WCGNNNNNNNCGW 1 cut(s) 304
PctI GAATGC 1 cut(s) 564
PfeI GAWTC 1 cut(s) 41
PscI ACATGT 1 cut(s) 552
Psp124BI GAGCTC 1 cut(s) 629
Psp6I CCWGG 1 cut(s) 354
PspFI CCCAGC 1 cut(s) 569
PspGI CCWGG 1 cut(s) 354
PspN4I GGNNCC 1 cut(s) 448
PspPI GGNCC 2 cut(s) 341, 447
PstI CTGCAG 1 cut(s) 638
PstNI CAGNNNCTG 1 cut(s) 50
PsuI RGATCY 1 cut(s) 311
PvuII CAGCTG 4 cut(s) 47, 438, 573, 639
RsaI GTAC 4 cut(s) 134, 222, 427, 687
RsaNI GTAC 4 cut(s) 133, 221, 426, 686
RseI CAYNNNNRTG 1 cut(s) 235
SacI GAGCTC 1 cut(s) 629
SapI GCTCTTC 1 cut(s) 617
SaqAI TTAA 1 cut(s) 204
Sau3AI GATC 3 cut(s) 285, 311, 651
Sau96I GGNCC 2 cut(s) 341, 447
ScrFI CCNGG 1 cut(s) 356
SduI GDGCHC 1 cut(s) 629
SfaNI GCATC 4 cut(s) 156, 227, 239, 249
SfcI CTRYAG 1 cut(s) 634
Sfr274I CTCGAG 2 cut(s) 170, 191
SinI GGWCC 1 cut(s) 447
SlaI CTCGAG 2 cut(s) 170, 191
SmiMI CAYNNNNRTG 1 cut(s) 235
SmlI CTYRAG 2 cut(s) 170, 191
SmoI CTYRAG 2 cut(s) 170, 191
Sse9I AATT 4 cut(s) 201, 206, 271, 712
SsiI CCGC 4 cut(s) 18, 95, 152, 580
SspMI CTAG 1 cut(s) 234
SstI GAGCTC 1 cut(s) 629
StyD4I CCNGG 1 cut(s) 354
StyI CCWWGG 1 cut(s) 681
TaaI ACNGT 2 cut(s) 425, 603
TaqI TCGA 5 cut(s) 171, 192, 213, 307, 598
TasI AATT 4 cut(s) 201, 206, 271, 712
TatI WGTACW 1 cut(s) 425
TauI GCSGC 1 cut(s) 155
TfiI GAWTC 1 cut(s) 41
Tru1I TTAA 1 cut(s) 204
Tru9I TTAA 1 cut(s) 204
TseFI GTSAC 1 cut(s) 458
Tsp45I GTSAC 1 cut(s) 458
TspDTI ATGAA 3 cut(s) 54, 199, 507
TspGWI ACGGA 1 cut(s) 69
VpaK11BI GGWCC 1 cut(s) 447
XapI RAATTY 2 cut(s) 206, 271
XbaI TCTAGA 1 cut(s) 233
XceI RCATGY 1 cut(s) 556
XhoI CTCGAG 2 cut(s) 170, 191
XspI CTAG 1 cut(s) 234
Zsp2I ATGCAT 1 cut(s) 242
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.