AT5G20740

Plant invertase/pectin methylesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0014269
Physical Location & Seq
Reverse (-)
7025565 .. 7026753
1189 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G20740.2

Sequence Viewer

Length: 618 bp
ATGGCTCCTACACAAAATCTCTTCCTTGTCGCTATTGCCTTCGCCGTCATTTTCACCGCCTCCACCGTACATGGTCGTCATAATGGTGCAGAAGATATCGTGCATTCCTCCTGCGAACACGCGAGCTATCCATCACTATGCGTCCGTACTCTTTCGTCATACTCCGGTCCAACCATCACCAACCGCCGCGATCTAGCTCAAGCCGCCATAAAAATCAGCCTCTCCCACGCTCAAAGCGCCGCGAAGAAACTCGCGGTGGTGCGAGACTCGGTGGGGAAGAAGAAGCAAGAGAAAGCGGCGCTTGTGGACTGTGTGGAGATGATTGGAGACTCTGTGGACGAGCTAAGCCGTACGCTCGGCGTTTTGAAGCACCTCCGTGTCTCTGGCGGTTCAGCTAAAGAGTTCCGGTGGCAGATGAGCAATGCACAGACGTGGGCTAGCGCGGCACTTACGGATGACGACACGTGTCTCGATGGGTTTCAAGGGATGGACGACGGTGAAATCAAAACGGAGGTGAAGCAGTGGATGACGAAAGTGGCCAGGGTTACGAGCAACGCGCTTTACATGGTCAACCAGCTAGATGAGACACGTGGCAAACCCCACGACGTACATCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0004857 GO:0008150 GO:0030234 GO:0043086 GO:0044092 GO:0048509 GO:0050789 GO:0050790 GO:0050793 GO:0065007 GO:0065009 GO:0098772 GO:1902183
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

205

Amino Acids

22.33

Weight (kDa)

7.09

Isoelectric Point (pI)

29.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 67 - 223 6.6e-40 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014269)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G20740 AT5G20740
fragaria_vesca FvH4_1g09220
malus_domestica MD02G1099200.v1.1 MD15G1222000.v1.1
prunus_persica Prupe.7G193700_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0095671 RchiOBHm_Chr2g0095741
rosa_laevigata RLG00000016589
rosa_multiflora Rmu_co8375965.1_g000001
rosa_roxburghii Rroxscaffold_2G00146130
rosa_rugosa Rorug02G0053000
rosa_samantha Rh2AG101300 Rh2BG102900 Rh2CG105300 Rh2DG102700
rosa_wichuraiana Rw2G007680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 6 cut(s) 122, 189, 242, 254, 443, 557
AciI CCGC 9 cut(s) 57, 184, 187, 204, 240, 254, 296, 387, 443
AcoI YGGCCR 1 cut(s) 537
AcvI CACGTG 2 cut(s) 465, 590
AfaI GTAC 4 cut(s) 69, 148, 352, 609
AflIII ACRYGT 3 cut(s) 462, 464, 587
AgsI TTSAA 2 cut(s) 367, 482
AjiI CACGTC 1 cut(s) 432
AjnI CCWGG 1 cut(s) 539
AleI CACNNNNGTG 2 cut(s) 375, 430
AluBI AGCT 5 cut(s) 126, 197, 343, 395, 577
AluI AGCT 5 cut(s) 126, 197, 343, 395, 577
Alw26I GTCTC 5 cut(s) 258, 321, 385, 473, 578
AoxI GGCC 1 cut(s) 537
AspLEI GCGC 4 cut(s) 239, 301, 443, 559
AspS9I GGNCC 1 cut(s) 167
AsuHPI GGTGA 4 cut(s) 46, 169, 509, 526
AsuNHI GCTAGC 1 cut(s) 437
AvaII GGWCC 1 cut(s) 167
BalI TGGCCA 1 cut(s) 539
BbrPI CACGTG 2 cut(s) 465, 590
BccI CCATC 4 cut(s) 139, 182, 467, 481
BceAI ACGGC 2 cut(s) 29, 333
BciT130I CCWGG 1 cut(s) 541
BcoDI GTCTC 5 cut(s) 258, 321, 385, 473, 578
BfaI CTAG 3 cut(s) 194, 438, 578
BfoI RGCGCY 2 cut(s) 240, 302
BisI GCNGC 5 cut(s) 187, 204, 240, 297, 444
BlpI GCTNAGC 1 cut(s) 344
BlsI GCNGC 5 cut(s) 188, 205, 241, 298, 445
Bme1390I CCNGG 1 cut(s) 541
Bme18I GGWCC 1 cut(s) 167
BmgBI CACGTC 1 cut(s) 432
BmgT120I GGNCC 1 cut(s) 167
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 1 cut(s) 541
BmtI GCTAGC 1 cut(s) 441
BoxI GACNNNNGTC 1 cut(s) 465
Bpu1102I GCTNAGC 1 cut(s) 344
BpuEI CTTGAG 1 cut(s) 183
BsaAI YACGTR 2 cut(s) 465, 590
BsaJI CCNNGG 1 cut(s) 540
BsaWI WCCGGW 2 cut(s) 164, 405
BsaXI ACNNNNNCTCC 2 cut(s) 318, 348
Bse3DI GCAATG 1 cut(s) 427
BseBI CCWGG 1 cut(s) 541
BseDI CCNNGG 1 cut(s) 540
BseGI GGATG 3 cut(s) 460, 492, 531
BseMI GCAATG 1 cut(s) 427
BsgI GTGCAG 1 cut(s) 108
Bsh1236I CGCG 6 cut(s) 122, 189, 242, 254, 443, 557
BshFI GGCC 1 cut(s) 539
BsiSI CCGG 2 cut(s) 165, 406
BsiWI CGTACG 1 cut(s) 350
BsmAI GTCTC 5 cut(s) 258, 321, 385, 473, 578
BsmI GAATGC 1 cut(s) 103
BsnI GGCC 1 cut(s) 539
Bsp143I GATC 1 cut(s) 190
Bsp1720I GCTNAGC 1 cut(s) 344
BspACI CCGC 9 cut(s) 57, 184, 187, 204, 240, 254, 296, 387, 443
BspANI GGCC 1 cut(s) 539
BspFNI CGCG 6 cut(s) 122, 189, 242, 254, 443, 557
BspLI GGNNCC 1 cut(s) 6
BspOI GCTAGC 1 cut(s) 441
BsrDI GCAATG 1 cut(s) 427
BssECI CCNNGG 1 cut(s) 540
BssMI GATC 1 cut(s) 190
Bst2UI CCWGG 1 cut(s) 541
Bst4CI ACNGT 3 cut(s) 67, 311, 497
Bst6I CTCTTC 1 cut(s) 26
BstBAI YACGTR 2 cut(s) 465, 590
BstC8I GCNNGC 2 cut(s) 124, 439
BstDEI CTNAG 1 cut(s) 344
BstF5I GGATG 3 cut(s) 460, 492, 531
BstFNI CGCG 6 cut(s) 122, 189, 242, 254, 443, 557
BstH2I RGCGCY 2 cut(s) 240, 302
BstHHI GCGC 4 cut(s) 239, 301, 443, 559
BstKTI GATC 1 cut(s) 193
BstMAI GTCTC 5 cut(s) 258, 321, 385, 473, 578
BstMBI GATC 1 cut(s) 190
BstMWI GCNNNNNNNGC 3 cut(s) 203, 236, 443
BstNI CCWGG 1 cut(s) 541
BstPAI GACNNNNGTC 1 cut(s) 465
BstSCI CCNGG 1 cut(s) 539
BstUI CGCG 6 cut(s) 122, 189, 242, 254, 443, 557
BsuRI GGCC 1 cut(s) 539
BtrI CACGTC 1 cut(s) 432
BtsCI GGATG 3 cut(s) 460, 492, 531
BtsI GCAGTG 1 cut(s) 527
BtsIMutI CAGTG 1 cut(s) 527
Cac8I GCNNGC 2 cut(s) 124, 439
CfoI GCGC 4 cut(s) 239, 301, 443, 559
Cfr13I GGNCC 1 cut(s) 167
CseI GACGC 1 cut(s) 130
Csp6I GTAC 4 cut(s) 68, 147, 351, 608
CviAII CATG 2 cut(s) 71, 565
CviQI GTAC 4 cut(s) 68, 147, 351, 608
DdeI CTNAG 1 cut(s) 344
DpnI GATC 1 cut(s) 192
DpnII GATC 1 cut(s) 190
EaeI YGGCCR 1 cut(s) 537
Eam1104I CTCTTC 1 cut(s) 26
EarI CTCTTC 1 cut(s) 26
Eco32I GATATC 1 cut(s) 97
Eco47I GGWCC 1 cut(s) 167
Eco72I CACGTG 2 cut(s) 465, 590
EcoRII CCWGG 1 cut(s) 539
EcoRV GATATC 1 cut(s) 97
FaeI CATG 2 cut(s) 74, 568
FaiI YATR 6 cut(s) 72, 81, 139, 160, 209, 566
FalI AAGNNNNNCTT 2 cut(s) 285, 317
FatI CATG 2 cut(s) 70, 564
Fnu4HI GCNGC 5 cut(s) 187, 204, 240, 297, 444
FokI GGATG 3 cut(s) 467, 499, 538
Fsp4HI GCNGC 5 cut(s) 187, 204, 240, 297, 444
FspBI CTAG 3 cut(s) 194, 438, 578
GlaI GCGC 4 cut(s) 238, 300, 442, 558
GluI GCNGC 5 cut(s) 187, 204, 240, 297, 444
HaeII RGCGCY 2 cut(s) 240, 302
HaeIII GGCC 1 cut(s) 539
HapII CCGG 2 cut(s) 165, 406
HgaI GACGC 1 cut(s) 130
HhaI GCGC 4 cut(s) 239, 301, 443, 559
Hin1II CATG 2 cut(s) 74, 568
Hin6I GCGC 4 cut(s) 237, 299, 441, 557
HinP1I GCGC 4 cut(s) 237, 299, 441, 557
HincII GTYRAC 1 cut(s) 571
HindII GTYRAC 1 cut(s) 571
HinfI GANTC 2 cut(s) 266, 329
HpaII CCGG 2 cut(s) 165, 406
HphI GGTGA 4 cut(s) 46, 169, 509, 526
Hpy166II GTNNAC 3 cut(s) 307, 337, 571
Hpy188III TCNNGA 1 cut(s) 470
Hpy8I GTNNAC 3 cut(s) 307, 337, 571
Hpy99I CGWCG 2 cut(s) 497, 608
HpyAV CCTTC 1 cut(s) 49
HpyCH4III ACNGT 3 cut(s) 67, 311, 497
HpyCH4IV ACGT 4 cut(s) 431, 464, 589, 606
HpyCH4V TGCA 3 cut(s) 89, 103, 425
HpyF10VI GCNNNNNNNGC 3 cut(s) 203, 236, 443
HpyF3I CTNAG 1 cut(s) 344
HpySE526I ACGT 4 cut(s) 431, 464, 589, 606
Hsp92II CATG 2 cut(s) 74, 568
HspAI GCGC 4 cut(s) 237, 299, 441, 557
Kzo9I GATC 1 cut(s) 190
LmnI GCTCC 1 cut(s) 10
LpnPI CCDG 7 cut(s) 124, 178, 369, 419, 526, 553, 587
MaeI CTAG 3 cut(s) 194, 438, 578
MaeII ACGT 4 cut(s) 431, 464, 589, 606
MaeIII GTNAC 1 cut(s) 544
MalI GATC 1 cut(s) 192
MboI GATC 1 cut(s) 190
MboII GAAGA 5 cut(s) 13, 104, 256, 289, 292
MlsI TGGCCA 1 cut(s) 539
MluNI TGGCCA 1 cut(s) 539
MlyI GAGTC 2 cut(s) 260, 323
MmeI TCCRAC 1 cut(s) 194
MnlI CCTC 5 cut(s) 70, 118, 230, 383, 505
Mox20I TGGCCA 1 cut(s) 539
MscI TGGCCA 1 cut(s) 539
MslI CAYNNNNRTG 4 cut(s) 84, 136, 375, 430
Msp20I TGGCCA 1 cut(s) 539
MspI CCGG 2 cut(s) 165, 406
MspR9I CCNGG 1 cut(s) 541
Mva1269I GAATGC 1 cut(s) 103
MvaI CCWGG 1 cut(s) 541
MvnI CGCG 6 cut(s) 122, 189, 242, 254, 443, 557
MwoI GCNNNNNNNGC 3 cut(s) 203, 236, 443
NdeII GATC 1 cut(s) 190
NheI GCTAGC 1 cut(s) 437
NlaIII CATG 2 cut(s) 74, 568
NlaIV GGNNCC 1 cut(s) 6
NmeAIII GCCGAG 1 cut(s) 336
OliI CACNNNNGTG 2 cut(s) 375, 430
PctI GAATGC 1 cut(s) 103
Pfl23II CGTACG 1 cut(s) 350
PkrI GCNGC 5 cut(s) 188, 205, 241, 298, 445
PleI GAGTC 2 cut(s) 260, 323
PmaCI CACGTG 2 cut(s) 465, 590
PmlI CACGTG 2 cut(s) 465, 590
PpsI GAGTC 2 cut(s) 260, 323
Ppu21I YACGTR 2 cut(s) 465, 590
PshAI GACNNNNGTC 1 cut(s) 465
Psp6I CCWGG 1 cut(s) 539
PspCI CACGTG 2 cut(s) 465, 590
PspGI CCWGG 1 cut(s) 539
PspLI CGTACG 1 cut(s) 350
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 1 cut(s) 167
RsaI GTAC 4 cut(s) 69, 148, 352, 609
RsaNI GTAC 4 cut(s) 68, 147, 351, 608
RseI CAYNNNNRTG 4 cut(s) 84, 136, 375, 430
SatI GCNGC 5 cut(s) 187, 204, 240, 297, 444
Sau3AI GATC 1 cut(s) 190
Sau96I GGNCC 1 cut(s) 167
SchI GAGTC 2 cut(s) 260, 323
ScrFI CCNGG 1 cut(s) 541
SinI GGWCC 1 cut(s) 167
SmiMI CAYNNNNRTG 4 cut(s) 84, 136, 375, 430
SmlI CTYRAG 1 cut(s) 198
SmoI CTYRAG 1 cut(s) 198
SsiI CCGC 9 cut(s) 57, 184, 187, 204, 240, 254, 296, 387, 443
SspMI CTAG 3 cut(s) 194, 438, 578
StyD4I CCNGG 1 cut(s) 539
TaaI ACNGT 3 cut(s) 67, 311, 497
TaiI ACGT 4 cut(s) 434, 467, 592, 609
TaqI TCGA 1 cut(s) 471
TauI GCSGC 5 cut(s) 189, 206, 242, 299, 446
TscAI CASTG 1 cut(s) 527
TspGWI ACGGA 4 cut(s) 134, 365, 467, 524
TspRI CASTG 1 cut(s) 527
VpaK11BI GGWCC 1 cut(s) 167
XspI CTAG 3 cut(s) 194, 438, 578
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.