FvH4_1g09220

21 kDa protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0014269
Physical Location & Seq
Reverse (-)
4921035 .. 4921628
594 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g09220.t1

Sequence Viewer

Length: 594 bp
ATGCGTTTCACTCTCCTCCTCCCCCTCCTCCCCTTCCTCCTCCTCCTCCTCCTCTCACACTCCTCCGCCGCACGTAAACCAACCTCCACCACCTCCCACCTCGTCCGTACTTCATGCCAACACGCCAGCTACCCCAACCTCTGCCTCCACACCCTCTCCACCTCCCCTGCCCAAACCCCTCGCGACCTCGCCCAAGCCGCCGTCGCCGTCTCCCTCTCACGCGCCCGTAAAGTCTCCGCCTTCCTCTCCCAGCTCTCCCACACCTCCTCCTCCAAACCAAAACGTCAGCGCGTGGCCCTCGGCGACTGCGTGGAGCAAGTCTCGGACTCCGTCGACGAGCTCAAGCGTACTCTGGAGGAGCTCCACCACCTCCATGAGGAGACGTTCCGGTGGCAGATGAGCAACGCCCAGACGTGGGTCAGCGCCGCCCTCACGAACGACGACACGTGTCTCGACGGGTTTCAGGAGGTCGACGGCGAGGTGAAGGCGGACGTCAAGAGGAAGATCACTAATGCTGCTAGGGTTACTAGTAACGCGCTCTATATGATCAACCGGCTAGACGAGAGCCGTGGACGGCCCAGATCTAAGCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0004857 GO:0008150 GO:0030234 GO:0043086 GO:0044092 GO:0048509 GO:0050789 GO:0050790 GO:0050793 GO:0065007 GO:0065009 GO:0098772 GO:1902183
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

198

Amino Acids

21.93

Weight (kDa)

9.38

Isoelectric Point (pI)

38.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 34 - 182 2.9e-44 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014269)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G20740 AT5G20740
fragaria_vesca FvH4_1g09220
malus_domestica MD02G1099200.v1.1 MD15G1222000.v1.1
prunus_persica Prupe.7G193700_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0095671 RchiOBHm_Chr2g0095741
rosa_laevigata RLG00000016589
rosa_multiflora Rmu_co8375965.1_g000001
rosa_roxburghii Rroxscaffold_2G00146130
rosa_rugosa Rorug02G0053000
rosa_samantha Rh2AG101300 Rh2BG102900 Rh2CG105300 Rh2DG102700
rosa_wichuraiana Rw2G007680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 495
AccI GTMKAC 2 cut(s) 333, 471
AccII CGCG 4 cut(s) 183, 222, 291, 536
AciI CCGC 6 cut(s) 66, 69, 198, 237, 426, 488
AcvI CACGTG 1 cut(s) 447
AcyI GRCGYC 1 cut(s) 492
AfaI GTAC 2 cut(s) 109, 349
AfiI CCNNNNNNNGG 3 cut(s) 376, 414, 415
AflIII ACRYGT 2 cut(s) 444, 446
AhlI ACTAGT 1 cut(s) 527
AjiI CACGTC 1 cut(s) 414
AluBI AGCT 4 cut(s) 129, 253, 340, 361
AluI AGCT 4 cut(s) 129, 253, 340, 361
Alw21I GWGCWC 2 cut(s) 342, 363
Alw26I GTCTC 5 cut(s) 214, 238, 325, 374, 455
AoxI GGCC 2 cut(s) 294, 575
ApeKI GCWGC 1 cut(s) 515
AspLEI GCGC 4 cut(s) 224, 291, 425, 538
AspS9I GGNCC 2 cut(s) 295, 576
AsuHPI GGTGA 1 cut(s) 493
BanII GRGCYC 2 cut(s) 342, 363
BbrPI CACGTG 1 cut(s) 447
Bbv12I GWGCWC 2 cut(s) 342, 363
BbvI GCAGC 1 cut(s) 502
BceAI ACGGC 5 cut(s) 185, 191, 490, 552, 590
BclI TGATCA 1 cut(s) 546
BcoDI GTCTC 5 cut(s) 214, 238, 325, 374, 455
BcuI ACTAGT 1 cut(s) 527
BfaI CTAG 3 cut(s) 519, 528, 557
BfoI RGCGCY 1 cut(s) 426
BglII AGATCT 1 cut(s) 581
BisI GCNGC 4 cut(s) 69, 198, 426, 516
BlsI GCNGC 4 cut(s) 70, 199, 427, 517
BmgBI CACGTC 1 cut(s) 414
BmgT120I GGNCC 2 cut(s) 295, 576
BoxI GACNNNNGTC 2 cut(s) 416, 447
BplI GAGNNNNNCTC 2 cut(s) 305, 337
BpmI CTGGAG 1 cut(s) 374
BpuEI CTTGAG 1 cut(s) 326
BsaAI YACGTR 2 cut(s) 74, 447
BsaHI GRCGYC 1 cut(s) 492
BsaJI CCNNGG 2 cut(s) 298, 568
BsaWI WCCGGW 1 cut(s) 387
BsaXI ACNNNNNCTCC 4 cut(s) 140, 170, 251, 281
Bsc4I CCNNNNNNNGG 3 cut(s) 376, 414, 415
Bse118I RCCGGY 1 cut(s) 552
BseDI CCNNGG 2 cut(s) 298, 568
BseLI CCNNNNNNNGG 3 cut(s) 376, 414, 415
BseXI GCAGC 1 cut(s) 502
BseYI CCCAGC 1 cut(s) 249
Bsh1236I CGCG 4 cut(s) 183, 222, 291, 536
BshFI GGCC 2 cut(s) 296, 577
BsiHKAI GWGCWC 2 cut(s) 342, 363
BsiSI CCGG 2 cut(s) 388, 553
BslI CCNNNNNNNGG 3 cut(s) 376, 414, 415
BsmAI GTCTC 5 cut(s) 214, 238, 325, 374, 455
BsmBI CGTCTC 2 cut(s) 214, 374
BsnI GGCC 2 cut(s) 296, 577
Bsp1286I GDGCHC 2 cut(s) 342, 363
Bsp143I GATC 3 cut(s) 504, 546, 581
Bsp68I TCGCGA 1 cut(s) 183
BspACI CCGC 6 cut(s) 66, 69, 198, 237, 426, 488
BspANI GGCC 2 cut(s) 296, 577
BspFNI CGCG 4 cut(s) 183, 222, 291, 536
BsrFI RCCGGY 1 cut(s) 552
BssAI RCCGGY 1 cut(s) 552
BssECI CCNNGG 2 cut(s) 298, 568
BssMI GATC 3 cut(s) 504, 546, 581
BssNI GRCGYC 1 cut(s) 492
BstACI GRCGYC 1 cut(s) 492
BstBAI YACGTR 2 cut(s) 74, 447
BstC8I GCNNGC 1 cut(s) 127
BstDEI CTNAG 1 cut(s) 585
BstDSI CCRYGG 1 cut(s) 568
BstENI CCTNNNNNAGG 1 cut(s) 374
BstFNI CGCG 4 cut(s) 183, 222, 291, 536
BstH2I RGCGCY 1 cut(s) 426
BstHHI GCGC 4 cut(s) 224, 291, 425, 538
BstKTI GATC 3 cut(s) 507, 549, 584
BstMAI GTCTC 5 cut(s) 214, 238, 325, 374, 455
BstMBI GATC 3 cut(s) 504, 546, 581
BstMWI GCNNNNNNNGC 2 cut(s) 197, 203
BstPAI GACNNNNGTC 2 cut(s) 416, 447
BstUI CGCG 4 cut(s) 183, 222, 291, 536
BstV1I GCAGC 1 cut(s) 502
BstX2I RGATCY 1 cut(s) 581
BstYI RGATCY 1 cut(s) 581
BsuRI GGCC 2 cut(s) 296, 577
BtgI CCRYGG 1 cut(s) 568
BtrI CACGTC 1 cut(s) 414
BtuMI TCGCGA 1 cut(s) 183
Cac8I GCNNGC 1 cut(s) 127
CfoI GCGC 4 cut(s) 224, 291, 425, 538
Cfr10I RCCGGY 1 cut(s) 552
Cfr13I GGNCC 2 cut(s) 295, 576
Csp6I GTAC 2 cut(s) 108, 348
CviAII CATG 2 cut(s) 114, 374
CviQI GTAC 2 cut(s) 108, 348
DdeI CTNAG 1 cut(s) 585
DpnI GATC 3 cut(s) 506, 548, 583
DpnII GATC 3 cut(s) 504, 546, 581
EciI GGCGGA 3 cut(s) 55, 226, 503
Ecl136II GAGCTC 2 cut(s) 340, 361
Eco24I GRGCYC 2 cut(s) 342, 363
Eco53kI GAGCTC 2 cut(s) 340, 361
Eco72I CACGTG 1 cut(s) 447
EcoICRI GAGCTC 2 cut(s) 340, 361
EcoNI CCTNNNNNAGG 1 cut(s) 374
EcoT38I GRGCYC 2 cut(s) 342, 363
Esp3I CGTCTC 2 cut(s) 214, 374
FaeI CATG 2 cut(s) 117, 377
FaiI YATR 4 cut(s) 115, 375, 543, 545
FatI CATG 2 cut(s) 113, 373
FbaI TGATCA 1 cut(s) 546
FblI GTMKAC 2 cut(s) 333, 471
Fnu4HI GCNGC 4 cut(s) 69, 198, 426, 516
FriOI GRGCYC 2 cut(s) 342, 363
Fsp4HI GCNGC 4 cut(s) 69, 198, 426, 516
FspBI CTAG 3 cut(s) 519, 528, 557
GlaI GCGC 4 cut(s) 223, 290, 424, 537
GluI GCNGC 4 cut(s) 69, 198, 426, 516
GsaI CCCAGC 1 cut(s) 253
GsuI CTGGAG 1 cut(s) 374
HaeII RGCGCY 1 cut(s) 426
HaeIII GGCC 2 cut(s) 296, 577
HapII CCGG 2 cut(s) 388, 553
HhaI GCGC 4 cut(s) 224, 291, 425, 538
Hin1I GRCGYC 1 cut(s) 492
Hin1II CATG 2 cut(s) 117, 377
Hin6I GCGC 4 cut(s) 222, 289, 423, 536
HinP1I GCGC 4 cut(s) 222, 289, 423, 536
HincII GTYRAC 2 cut(s) 334, 472
HindII GTYRAC 2 cut(s) 334, 472
HinfI GANTC 1 cut(s) 326
HpaII CCGG 2 cut(s) 388, 553
HphI GGTGA 1 cut(s) 493
Hpy166II GTNNAC 4 cut(s) 77, 334, 472, 572
Hpy188I TCNGA 1 cut(s) 325
Hpy188III TCNNGA 6 cut(s) 182, 353, 433, 452, 464, 496
Hpy8I GTNNAC 4 cut(s) 77, 334, 472, 572
Hpy99I CGWCG 6 cut(s) 206, 335, 338, 443, 458, 476
HpyAV CCTTC 3 cut(s) 43, 250, 478
HpyCH4IV ACGT 6 cut(s) 73, 283, 383, 413, 446, 492
HpyF10VI GCNNNNNNNGC 2 cut(s) 197, 203
HpyF3I CTNAG 1 cut(s) 585
HpySE526I ACGT 6 cut(s) 73, 283, 383, 413, 446, 492
Hsp92I GRCGYC 1 cut(s) 492
Hsp92II CATG 2 cut(s) 117, 377
HspAI GCGC 4 cut(s) 222, 289, 423, 536
Ksp22I TGATCA 1 cut(s) 546
Kzo9I GATC 3 cut(s) 504, 546, 581
LmnI GCTCC 3 cut(s) 313, 358, 366
LpnPI CCDG 8 cut(s) 139, 180, 263, 338, 401, 422, 449, 566
Lsp1109I GCAGC 1 cut(s) 502
MaeI CTAG 3 cut(s) 519, 528, 557
MaeII ACGT 6 cut(s) 73, 283, 383, 413, 446, 492
MaeIII GTNAC 2 cut(s) 523, 530
MalI GATC 3 cut(s) 506, 548, 583
MboI GATC 3 cut(s) 504, 546, 581
MboII GAAGA 1 cut(s) 514
MflI RGATCY 1 cut(s) 581
MhlI GDGCHC 2 cut(s) 342, 363
MlyI GAGTC 1 cut(s) 320
MslI CAYNNNNRTG 1 cut(s) 372
MspI CCGG 2 cut(s) 388, 553
MvnI CGCG 4 cut(s) 183, 222, 291, 536
MwoI GCNNNNNNNGC 2 cut(s) 197, 203
NdeII GATC 3 cut(s) 504, 546, 581
NlaIII CATG 2 cut(s) 117, 377
NmeAIII GCCGAG 1 cut(s) 279
NruI TCGCGA 1 cut(s) 183
PcsI WCGNNNNNNNCGW 1 cut(s) 306
PflFI GACNNNGTC 1 cut(s) 329
PkrI GCNGC 4 cut(s) 70, 199, 427, 517
PleI GAGTC 1 cut(s) 320
PmaCI CACGTG 1 cut(s) 447
PmlI CACGTG 1 cut(s) 447
PpsI GAGTC 1 cut(s) 320
Ppu21I YACGTR 2 cut(s) 74, 447
PshAI GACNNNNGTC 2 cut(s) 416, 447
Psp124BI GAGCTC 2 cut(s) 342, 363
PspCI CACGTG 1 cut(s) 447
PspFI CCCAGC 1 cut(s) 249
PspPI GGNCC 2 cut(s) 295, 576
PsuI RGATCY 1 cut(s) 581
PsyI GACNNNGTC 1 cut(s) 329
RruI TCGCGA 1 cut(s) 183
RsaI GTAC 2 cut(s) 109, 349
RsaNI GTAC 2 cut(s) 108, 348
RseI CAYNNNNRTG 1 cut(s) 372
SacI GAGCTC 2 cut(s) 342, 363
SalI GTCGAC 2 cut(s) 332, 470
SatI GCNGC 4 cut(s) 69, 198, 426, 516
Sau3AI GATC 3 cut(s) 504, 546, 581
Sau96I GGNCC 2 cut(s) 295, 576
SchI GAGTC 1 cut(s) 320
SduI GDGCHC 2 cut(s) 342, 363
SgrDI CGTCGACG 1 cut(s) 332
SmiMI CAYNNNNRTG 1 cut(s) 372
SmlI CTYRAG 1 cut(s) 341
SmoI CTYRAG 1 cut(s) 341
SpeI ACTAGT 1 cut(s) 527
SsiI CCGC 6 cut(s) 66, 69, 198, 237, 426, 488
SspMI CTAG 3 cut(s) 519, 528, 557
SstI GAGCTC 2 cut(s) 342, 363
TaiI ACGT 6 cut(s) 76, 286, 386, 416, 449, 495
TaqI TCGA 3 cut(s) 333, 453, 471
TauI GCSGC 3 cut(s) 71, 200, 428
TseI GCWGC 1 cut(s) 515
TspDTI ATGAA 1 cut(s) 102
TspGWI ACGGA 2 cut(s) 95, 319
Tth111I GACNNNGTC 1 cut(s) 329
XagI CCTNNNNNAGG 1 cut(s) 374
XmiI GTMKAC 2 cut(s) 333, 471
XspI CTAG 3 cut(s) 519, 528, 557
ZraI GACGTC 1 cut(s) 493
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.