MD15G1222000.v1.1

21 kDa protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0014269
Physical Location & Seq
Reverse (-)
18008883 .. 18009479
597 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1222000.v1.1.491

Sequence Viewer

Length: 597 bp
ATGCGTTTCCTACTCTCTCTTCTCCCTCTCCTCGTCCTCCTCTTCTGCTCGCTGTACTCCGCCTCCGCCGCAGGGAAACACCACCCAGCATCAAATATCGTCCGTTCATCATGTCGCCACGCCACTTACCCCAAAATCTGCCTCCGCACTCTATCCAACTACGCCGGCCCCGCCAAGACCCCCCGCGACCTCGCCCAGGCCGCTGTGAACGTTAGCCTCGCCCGCTCACTCCGCGTCTCCGGCTTCCTCGCCCAGCTCTCCGCCTCCTTCAAGGGGACGAAGCTGCAGCTCTCGGCGATCGGCGACTGCGTGGACCTGATGTCGACGTCGGTGGACGAGCTGAGCGAGACGCTCGCCGAGCTGCACCACCTCCGGAAGGAGACGTTTCGATTCCAGATGAGCAATGCCCAGACGTGGCTGAGCGCCGCCCTCACGGATGACGAGACGTGTCTCGATGGGATTCAGGAGGCGAATGTGAAGGCCAAGGCCGTGGATGTGAAGCGGAAGATTACGAATTCGGCTAGGGTTACGAGCAACGCGCTGTACCTGATCAATCGCCTCGACAAGAGCAGCAGATGGCCCAGATCTGGACCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0004857 GO:0008150 GO:0030234 GO:0043086 GO:0044092 GO:0048509 GO:0050789 GO:0050790 GO:0050793 GO:0065007 GO:0065009 GO:0098772 GO:1902183
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

199

Amino Acids

21.68

Weight (kDa)

9.67

Isoelectric Point (pI)

36.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 33 - 183 3.5e-37 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014269)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G20740 AT5G20740
fragaria_vesca FvH4_1g09220
malus_domestica MD02G1099200.v1.1 MD15G1222000.v1.1
prunus_persica Prupe.7G193700_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0095671 RchiOBHm_Chr2g0095741
rosa_laevigata RLG00000016589
rosa_multiflora Rmu_co8375965.1_g000001
rosa_roxburghii Rroxscaffold_2G00146130
rosa_rugosa Rorug02G0053000
rosa_samantha Rh2AG101300 Rh2BG102900 Rh2CG105300 Rh2DG102700
rosa_wichuraiana Rw2G007680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 329
AccBSI CCGCTC 1 cut(s) 225
AccI GTMKAC 1 cut(s) 323
AccII CGCG 3 cut(s) 186, 234, 539
AccIII TCCGGA 1 cut(s) 372
AclI AACGTT 1 cut(s) 210
AcsI RAATTY 1 cut(s) 514
AcyI GRCGYC 1 cut(s) 326
AfaI GTAC 2 cut(s) 56, 545
AfiI CCNNNNNNNGG 6 cut(s) 72, 196, 273, 376, 414, 587
AflIII ACRYGT 1 cut(s) 446
AgsI TTSAA 1 cut(s) 271
AhdI GACNNNNNGTC 1 cut(s) 319
AjiI CACGTC 2 cut(s) 414, 447
AjnI CCWGG 1 cut(s) 195
AluBI AGCT 5 cut(s) 256, 283, 289, 340, 361
AluI AGCT 5 cut(s) 256, 283, 289, 340, 361
Alw26I GTCTC 5 cut(s) 241, 341, 374, 437, 455
Aor13HI TCCGGA 1 cut(s) 372
AoxI GGCC 5 cut(s) 166, 198, 480, 486, 578
ApeKI GCWGC 4 cut(s) 283, 286, 361, 570
ApoI RAATTY 1 cut(s) 514
AspLEI GCGC 2 cut(s) 425, 541
AspS9I GGNCC 4 cut(s) 167, 313, 579, 590
AvaII GGWCC 2 cut(s) 313, 590
BbvI GCAGC 4 cut(s) 270, 298, 348, 582
BccI CCATC 2 cut(s) 449, 570
BceAI ACGGC 1 cut(s) 473
BciT130I CCWGG 1 cut(s) 197
BclI TGATCA 1 cut(s) 549
BcoDI GTCTC 5 cut(s) 241, 341, 374, 437, 455
BfaI CTAG 1 cut(s) 522
BfmI CTRYAG 1 cut(s) 284
BfoI RGCGCY 1 cut(s) 426
BglII AGATCT 1 cut(s) 584
BisI GCNGC 7 cut(s) 69, 201, 284, 287, 362, 426, 571
BlpI GCTNAGC 2 cut(s) 341, 419
BlsI GCNGC 7 cut(s) 70, 202, 285, 288, 363, 427, 572
Bme1390I CCNGG 1 cut(s) 197
Bme18I GGWCC 2 cut(s) 313, 590
BmeRI GACNNNNNGTC 1 cut(s) 319
BmgBI CACGTC 2 cut(s) 414, 447
BmgT120I GGNCC 4 cut(s) 167, 313, 579, 590
BmiI GGNNCC 1 cut(s) 169
BmrFI CCNGG 1 cut(s) 197
BmsI GCATC 1 cut(s) 98
Bpu1102I GCTNAGC 2 cut(s) 341, 419
BsaHI GRCGYC 1 cut(s) 326
BsaJI CCNNGG 3 cut(s) 195, 483, 489
BsaWI WCCGGW 1 cut(s) 372
BsaXI ACNNNNNCTCC 2 cut(s) 47, 77
Bsc4I CCNNNNNNNGG 6 cut(s) 72, 196, 273, 376, 414, 587
Bse118I RCCGGY 1 cut(s) 164
Bse3DI GCAATG 1 cut(s) 409
BseAI TCCGGA 1 cut(s) 372
BseBI CCWGG 1 cut(s) 197
BseDI CCNNGG 3 cut(s) 195, 483, 489
BseGI GGATG 2 cut(s) 442, 499
BseLI CCNNNNNNNGG 6 cut(s) 72, 196, 273, 376, 414, 587
BseMI GCAATG 1 cut(s) 409
BseMII CTCAG 2 cut(s) 332, 410
BseRI GAGGAG 2 cut(s) 20, 29
BseXI GCAGC 4 cut(s) 270, 298, 348, 582
BseYI CCCAGC 2 cut(s) 85, 252
BsgI GTGCAG 1 cut(s) 347
Bsh1236I CGCG 3 cut(s) 186, 234, 539
Bsh1285I CGRYCG 1 cut(s) 300
BshFI GGCC 5 cut(s) 168, 200, 482, 488, 580
BsiEI CGRYCG 1 cut(s) 300
BsiSI CCGG 3 cut(s) 165, 240, 373
BslFI GGGAC 1 cut(s) 289
BslI CCNNNNNNNGG 6 cut(s) 72, 196, 273, 376, 414, 587
BsmAI GTCTC 5 cut(s) 241, 341, 374, 437, 455
BsmBI CGTCTC 4 cut(s) 241, 341, 374, 437
BsmFI GGGAC 1 cut(s) 289
BsnI GGCC 5 cut(s) 168, 200, 482, 488, 580
Bsp13I TCCGGA 1 cut(s) 372
Bsp143I GATC 3 cut(s) 297, 549, 584
Bsp1720I GCTNAGC 2 cut(s) 341, 419
BspANI GGCC 5 cut(s) 168, 200, 482, 488, 580
BspCNI CTCAG 2 cut(s) 333, 411
BspEI TCCGGA 1 cut(s) 372
BspFNI CGCG 3 cut(s) 186, 234, 539
BspLI GGNNCC 1 cut(s) 169
BspMAI CTGCAG 1 cut(s) 288
BsrBI CCGCTC 1 cut(s) 225
BsrDI GCAATG 1 cut(s) 409
BsrFI RCCGGY 1 cut(s) 164
BssAI RCCGGY 1 cut(s) 164
BssECI CCNNGG 3 cut(s) 195, 483, 489
BssMI GATC 3 cut(s) 297, 549, 584
BssNI GRCGYC 1 cut(s) 326
BssT1I CCWWGG 1 cut(s) 483
Bst2UI CCWGG 1 cut(s) 197
Bst6I CTCTTC 2 cut(s) 24, 47
BstACI GRCGYC 1 cut(s) 326
BstC8I GCNNGC 4 cut(s) 50, 166, 223, 354
BstDEI CTNAG 2 cut(s) 341, 419
BstDSI CCRYGG 1 cut(s) 489
BstENI CCTNNNNNAGG 2 cut(s) 194, 374
BstF5I GGATG 2 cut(s) 442, 499
BstFNI CGCG 3 cut(s) 186, 234, 539
BstH2I RGCGCY 1 cut(s) 426
BstHHI GCGC 2 cut(s) 425, 541
BstKTI GATC 3 cut(s) 300, 552, 587
BstMAI GTCTC 5 cut(s) 241, 341, 374, 437, 455
BstMBI GATC 3 cut(s) 297, 549, 584
BstMCI CGRYCG 1 cut(s) 300
BstMWI GCNNNNNNNGC 7 cut(s) 68, 170, 200, 222, 231, 240, 358
BstNI CCWGG 1 cut(s) 197
BstSCI CCNGG 1 cut(s) 195
BstSFI CTRYAG 1 cut(s) 284
BstUI CGCG 3 cut(s) 186, 234, 539
BstV1I GCAGC 4 cut(s) 270, 298, 348, 582
BstX2I RGATCY 1 cut(s) 584
BstXI CCANNNNNNTGG 1 cut(s) 490
BstYI RGATCY 1 cut(s) 584
BsuRI GGCC 5 cut(s) 168, 200, 482, 488, 580
BtgI CCRYGG 1 cut(s) 489
BtrI CACGTC 2 cut(s) 414, 447
BtsCI GGATG 2 cut(s) 442, 499
Cac8I GCNNGC 4 cut(s) 50, 166, 223, 354
CfoI GCGC 2 cut(s) 425, 541
Cfr10I RCCGGY 1 cut(s) 164
Cfr13I GGNCC 4 cut(s) 167, 313, 579, 590
CseI GACGC 2 cut(s) 223, 358
Csp6I GTAC 2 cut(s) 55, 544
CviAII CATG 1 cut(s) 111
CviQI GTAC 2 cut(s) 55, 544
DdeI CTNAG 2 cut(s) 341, 419
DpnI GATC 3 cut(s) 299, 551, 586
DpnII GATC 3 cut(s) 297, 549, 584
DriI GACNNNNNGTC 1 cut(s) 319
Eam1104I CTCTTC 2 cut(s) 24, 47
Eam1105I GACNNNNNGTC 1 cut(s) 319
EarI CTCTTC 2 cut(s) 24, 47
EciI GGCGGA 3 cut(s) 49, 55, 250
Eco130I CCWWGG 1 cut(s) 483
Eco47I GGWCC 2 cut(s) 313, 590
EcoNI CCTNNNNNAGG 2 cut(s) 194, 374
EcoRI GAATTC 1 cut(s) 514
EcoRII CCWGG 1 cut(s) 195
EcoT14I CCWWGG 1 cut(s) 483
ErhI CCWWGG 1 cut(s) 483
Esp3I CGTCTC 4 cut(s) 241, 341, 374, 437
FaeI CATG 1 cut(s) 114
FaiI YATR 1 cut(s) 112
FaqI GGGAC 1 cut(s) 289
FatI CATG 1 cut(s) 110
FauI CCCGC 3 cut(s) 178, 191, 230
FbaI TGATCA 1 cut(s) 549
FblI GTMKAC 1 cut(s) 323
Fnu4HI GCNGC 7 cut(s) 69, 201, 284, 287, 362, 426, 571
FokI GGATG 2 cut(s) 449, 506
Fsp4HI GCNGC 7 cut(s) 69, 201, 284, 287, 362, 426, 571
FspBI CTAG 1 cut(s) 522
GlaI GCGC 2 cut(s) 424, 540
GluI GCNGC 7 cut(s) 69, 201, 284, 287, 362, 426, 571
GsaI CCCAGC 2 cut(s) 89, 256
HaeII RGCGCY 1 cut(s) 426
HaeIII GGCC 5 cut(s) 168, 200, 482, 488, 580
HapII CCGG 3 cut(s) 165, 240, 373
HgaI GACGC 2 cut(s) 223, 358
HhaI GCGC 2 cut(s) 425, 541
Hin1I GRCGYC 1 cut(s) 326
Hin1II CATG 1 cut(s) 114
Hin6I GCGC 2 cut(s) 423, 539
HinP1I GCGC 2 cut(s) 423, 539
HincII GTYRAC 1 cut(s) 324
HindII GTYRAC 1 cut(s) 324
HinfI GANTC 2 cut(s) 390, 460
HpaII CCGG 3 cut(s) 165, 240, 373
Hpy166II GTNNAC 4 cut(s) 208, 313, 324, 334
Hpy188III TCNNGA 5 cut(s) 373, 394, 452, 464, 588
Hpy8I GTNNAC 4 cut(s) 208, 313, 324, 334
Hpy99I CGWCG 2 cut(s) 328, 331
HpyAV CCTTC 3 cut(s) 277, 370, 472
HpyCH4IV ACGT 5 cut(s) 210, 326, 383, 413, 446
HpyCH4V TGCA 2 cut(s) 286, 364
HpyF10VI GCNNNNNNNGC 7 cut(s) 68, 170, 200, 222, 231, 240, 358
HpyF3I CTNAG 2 cut(s) 341, 419
HpySE526I ACGT 5 cut(s) 210, 326, 383, 413, 446
Hsp92I GRCGYC 1 cut(s) 326
Hsp92II CATG 1 cut(s) 114
HspAI GCGC 2 cut(s) 423, 539
Kpn2I TCCGGA 1 cut(s) 372
KroI GCCGGC 1 cut(s) 164
KroNI GCCGGC 1 cut(s) 166
Ksp22I TGATCA 1 cut(s) 549
Kzo9I GATC 3 cut(s) 297, 549, 584
Lsp1109I GCAGC 4 cut(s) 270, 298, 348, 582
LweI GCATC 1 cut(s) 98
MaeI CTAG 1 cut(s) 522
MaeII ACGT 5 cut(s) 210, 326, 383, 413, 446
MaeIII GTNAC 1 cut(s) 526
MalI GATC 3 cut(s) 299, 551, 586
MbiI CCGCTC 1 cut(s) 225
MboI GATC 3 cut(s) 297, 549, 584
MboII GAAGA 3 cut(s) 11, 34, 517
MflI RGATCY 1 cut(s) 584
MluCI AATT 1 cut(s) 514
MmeI TCCRAC 1 cut(s) 180
MroI TCCGGA 1 cut(s) 372
MroNI GCCGGC 1 cut(s) 164
MspA1I CMGCKG 1 cut(s) 203
MspI CCGG 3 cut(s) 165, 240, 373
MspR9I CCNGG 1 cut(s) 197
MvaI CCWGG 1 cut(s) 197
MvnI CGCG 3 cut(s) 186, 234, 539
MwoI GCNNNNNNNGC 7 cut(s) 68, 170, 200, 222, 231, 240, 358
NaeI GCCGGC 1 cut(s) 166
NdeII GATC 3 cut(s) 297, 549, 584
NgoMIV GCCGGC 1 cut(s) 164
NlaIII CATG 1 cut(s) 114
NlaIV GGNNCC 1 cut(s) 169
NmeAIII GCCGAG 2 cut(s) 272, 382
PcsI WCGNNNNNNNCGW 2 cut(s) 306, 342
PdiI GCCGGC 1 cut(s) 166
PfeI GAWTC 2 cut(s) 390, 460
PkrI GCNGC 7 cut(s) 70, 202, 285, 288, 363, 427, 572
Ple19I CGATCG 1 cut(s) 300
Psp1406I AACGTT 1 cut(s) 210
Psp6I CCWGG 1 cut(s) 195
PspFI CCCAGC 2 cut(s) 85, 252
PspGI CCWGG 1 cut(s) 195
PspN4I GGNNCC 1 cut(s) 169
PspPI GGNCC 4 cut(s) 167, 313, 579, 590
PstI CTGCAG 1 cut(s) 288
PsuI RGATCY 1 cut(s) 584
PvuI CGATCG 1 cut(s) 300
RsaI GTAC 2 cut(s) 56, 545
RsaNI GTAC 2 cut(s) 55, 544
SalI GTCGAC 1 cut(s) 322
SatI GCNGC 7 cut(s) 69, 201, 284, 287, 362, 426, 571
Sau3AI GATC 3 cut(s) 297, 549, 584
Sau96I GGNCC 4 cut(s) 167, 313, 579, 590
ScrFI CCNGG 1 cut(s) 197
SfaNI GCATC 1 cut(s) 98
SfcI CTRYAG 1 cut(s) 284
SinI GGWCC 2 cut(s) 313, 590
Sse9I AATT 1 cut(s) 514
SspMI CTAG 1 cut(s) 522
StyD4I CCNGG 1 cut(s) 195
StyI CCWWGG 1 cut(s) 483
TaiI ACGT 5 cut(s) 213, 329, 386, 416, 449
TaqI TCGA 4 cut(s) 323, 388, 453, 561
TasI AATT 1 cut(s) 514
TatI WGTACW 1 cut(s) 54
TauI GCSGC 3 cut(s) 71, 203, 428
TfiI GAWTC 2 cut(s) 390, 460
TseI GCWGC 4 cut(s) 283, 286, 361, 570
TspDTI ATGAA 1 cut(s) 96
TspGWI ACGGA 2 cut(s) 92, 449
VpaK11BI GGWCC 2 cut(s) 313, 590
XagI CCTNNNNNAGG 2 cut(s) 194, 374
XapI RAATTY 1 cut(s) 514
XmiI GTMKAC 1 cut(s) 323
XspI CTAG 1 cut(s) 522
ZraI GACGTC 1 cut(s) 327
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.