Rh2DG102700

21 kDa protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0014269
Physical Location & Seq
Reverse (-)
8348550 .. 8349736
1187 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG102700.1

Sequence Viewer

Length: 585 bp
ATGCGTTTCACTCTCCTCCCTCTCCTCCTCCTTCTCTCATACTCCGCCGCCGCACGTAAACCAAGCTCCCACAACGCCACCACCTCCTCCTCCCACCTCGTCCGTACGTCATGCCAACACGCCAGCTACCCCAACCTCTGCCTCCACACGCTCTCCGCCTCCCCGGCCCAGACCCCTCGCGACCTGGCCCAGGCTGCCGTCGGCGTCTCCCTCTCACGCGCCCGTAAAGTCTCCGCCTTCCTCTCCCAGCTTTCCCATTCCTCCTCCCCGAAGCGGCAGCGCGTGGCGGTCGGCGACTGCGTGGAGCAGGTCTCGGACTCCGTCGACGATCTGAGCCGCACGCTGGAGGAGCTCCACCACCTCCACGAGGAGACGTTCCGGTGGCAGATGAGCAACGCCCAGACGTGGGTCAGCGCCGCCCTCACGAACGACGACACGTGTCTCGACGGGTTCCAGGAGGTCGACGGCAAGGTGAAGGCGGACGTCAAGAGGAAGATTACCAATGCTGCTAGGGTTACCAGTAACGCGCTCTACATGATCAATCGGCTCGACGAGAGCCGCGGACGGCCCAGATCTAAACCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0004857 GO:0008150 GO:0030234 GO:0043086 GO:0044092 GO:0048509 GO:0050789 GO:0050790 GO:0050793 GO:0065007 GO:0065009 GO:0098772 GO:1902183
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

194

Amino Acids

21.38

Weight (kDa)

9.38

Isoelectric Point (pI)

42.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 33 - 179 3.7e-43 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014269)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G20740 AT5G20740
fragaria_vesca FvH4_1g09220
malus_domestica MD02G1099200.v1.1 MD15G1222000.v1.1
prunus_persica Prupe.7G193700_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0095671 RchiOBHm_Chr2g0095741
rosa_laevigata RLG00000016589
rosa_multiflora Rmu_co8375965.1_g000001
rosa_roxburghii Rroxscaffold_2G00146130
rosa_rugosa Rorug02G0053000
rosa_samantha Rh2AG101300 Rh2BG102900 Rh2CG105300 Rh2DG102700
rosa_wichuraiana Rw2G007680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 486
Acc36I ACCTGC 1 cut(s) 298
AccI GTMKAC 2 cut(s) 324, 462
AccII CGCG 5 cut(s) 180, 219, 282, 527, 561
AcvI CACGTG 1 cut(s) 438
AcyI GRCGYC 2 cut(s) 204, 483
AfaI GTAC 1 cut(s) 106
AfiI CCNNNNNNNGG 6 cut(s) 190, 273, 343, 367, 405, 406
AflIII ACRYGT 2 cut(s) 435, 437
AjiI CACGTC 1 cut(s) 405
AjnI CCWGG 3 cut(s) 183, 189, 453
AluBI AGCT 4 cut(s) 66, 126, 250, 352
AluI AGCT 4 cut(s) 66, 126, 250, 352
Alw21I GWGCWC 1 cut(s) 354
Alw26I GTCTC 5 cut(s) 211, 235, 316, 365, 446
AoxI GGCC 3 cut(s) 165, 186, 566
ApeKI GCWGC 3 cut(s) 194, 277, 506
AspLEI GCGC 4 cut(s) 221, 282, 416, 529
AspS9I GGNCC 3 cut(s) 166, 187, 567
AsuC2I CCSGG 1 cut(s) 164
AsuHPI GGTGA 1 cut(s) 484
BanII GRGCYC 1 cut(s) 354
BauI CACGAG 1 cut(s) 365
BbrPI CACGTG 1 cut(s) 438
Bbv12I GWGCWC 1 cut(s) 354
BbvI GCAGC 3 cut(s) 181, 289, 493
BceAI ACGGC 3 cut(s) 182, 481, 581
BciT130I CCWGG 3 cut(s) 185, 191, 455
BclI TGATCA 1 cut(s) 537
BcnI CCSGG 1 cut(s) 164
BcoDI GTCTC 5 cut(s) 211, 235, 316, 365, 446
BfaI CTAG 1 cut(s) 510
BfoI RGCGCY 1 cut(s) 417
BfuAI ACCTGC 1 cut(s) 298
BglI GCCNNNNNGGC 1 cut(s) 164
BglII AGATCT 1 cut(s) 572
BisI GCNGC 9 cut(s) 48, 51, 195, 275, 278, 337, 417, 507, 559
BlsI GCNGC 9 cut(s) 49, 52, 196, 276, 279, 338, 418, 508, 560
Bme1390I CCNGG 4 cut(s) 164, 185, 191, 455
BmgBI CACGTC 1 cut(s) 405
BmgT120I GGNCC 3 cut(s) 166, 187, 567
BmiI GGNNCC 1 cut(s) 452
BmrFI CCNGG 4 cut(s) 164, 185, 191, 455
BoxI GACNNNNGTC 2 cut(s) 407, 438
BplI GAGNNNNNCTC 2 cut(s) 296, 328
BpmI CTGGAG 1 cut(s) 365
BpuMI CCSGG 1 cut(s) 164
BsaAI YACGTR 2 cut(s) 56, 438
BsaHI GRCGYC 2 cut(s) 204, 483
BsaI GGTCTC 1 cut(s) 316
BsaJI CCNNGG 3 cut(s) 162, 189, 559
BsaWI WCCGGW 1 cut(s) 378
BsaXI ACNNNNNCTCC 2 cut(s) 137, 167
Bsc4I CCNNNNNNNGG 6 cut(s) 190, 273, 343, 367, 405, 406
Bse1I ACTGG 1 cut(s) 519
BseBI CCWGG 3 cut(s) 185, 191, 455
BseDI CCNNGG 3 cut(s) 162, 189, 559
BseLI CCNNNNNNNGG 6 cut(s) 190, 273, 343, 367, 405, 406
BseMII CTCAG 1 cut(s) 323
BseNI ACTGG 1 cut(s) 519
BseRI GAGGAG 8 cut(s) 5, 14, 17, 76, 79, 253, 362, 383
BseXI GCAGC 3 cut(s) 181, 289, 493
BseYI CCCAGC 1 cut(s) 246
Bsh1236I CGCG 5 cut(s) 180, 219, 282, 527, 561
Bsh1285I CGRYCG 1 cut(s) 291
BshFI GGCC 3 cut(s) 167, 188, 568
BsiEI CGRYCG 1 cut(s) 291
BsiHKAI GWGCWC 1 cut(s) 354
BsiSI CCGG 2 cut(s) 164, 379
BsiWI CGTACG 1 cut(s) 104
BslI CCNNNNNNNGG 6 cut(s) 190, 273, 343, 367, 405, 406
BsmAI GTCTC 5 cut(s) 211, 235, 316, 365, 446
BsmBI CGTCTC 2 cut(s) 211, 365
BsnI GGCC 3 cut(s) 167, 188, 568
Bso31I GGTCTC 1 cut(s) 316
Bsp1286I GDGCHC 1 cut(s) 354
Bsp143I GATC 3 cut(s) 328, 537, 572
Bsp68I TCGCGA 1 cut(s) 180
BspANI GGCC 3 cut(s) 167, 188, 568
BspCNI CTCAG 1 cut(s) 324
BspFNI CGCG 5 cut(s) 180, 219, 282, 527, 561
BspLI GGNNCC 1 cut(s) 452
BspMI ACCTGC 1 cut(s) 298
BspTNI GGTCTC 1 cut(s) 316
BsrI ACTGG 1 cut(s) 519
BssECI CCNNGG 3 cut(s) 162, 189, 559
BssMI GATC 3 cut(s) 328, 537, 572
BssNI GRCGYC 2 cut(s) 204, 483
BssSI CACGAG 1 cut(s) 365
Bst2BI CACGAG 1 cut(s) 365
Bst2UI CCWGG 3 cut(s) 185, 191, 455
BstACI GRCGYC 2 cut(s) 204, 483
BstBAI YACGTR 2 cut(s) 56, 438
BstC8I GCNNGC 2 cut(s) 124, 341
BstDEI CTNAG 1 cut(s) 332
BstDSI CCRYGG 1 cut(s) 559
BstEII GGTNACC 1 cut(s) 514
BstENI CCTNNNNNAGG 2 cut(s) 188, 365
BstFNI CGCG 5 cut(s) 180, 219, 282, 527, 561
BstH2I RGCGCY 1 cut(s) 417
BstHHI GCGC 4 cut(s) 221, 282, 416, 529
BstKTI GATC 3 cut(s) 331, 540, 575
BstMAI GTCTC 5 cut(s) 211, 235, 316, 365, 446
BstMBI GATC 3 cut(s) 328, 537, 572
BstMCI CGRYCG 1 cut(s) 291
BstMWI GCNNNNNNNGC 3 cut(s) 164, 194, 349
BstNI CCWGG 3 cut(s) 185, 191, 455
BstPAI GACNNNNGTC 2 cut(s) 407, 438
BstPI GGTNACC 1 cut(s) 514
BstSCI CCNGG 4 cut(s) 162, 183, 189, 453
BstUI CGCG 5 cut(s) 180, 219, 282, 527, 561
BstV1I GCAGC 3 cut(s) 181, 289, 493
BstX2I RGATCY 1 cut(s) 572
BstYI RGATCY 1 cut(s) 572
BsuRI GGCC 3 cut(s) 167, 188, 568
BtgI CCRYGG 1 cut(s) 559
BtrI CACGTC 1 cut(s) 405
BtuMI TCGCGA 1 cut(s) 180
BveI ACCTGC 1 cut(s) 298
Cac8I GCNNGC 2 cut(s) 124, 341
CfoI GCGC 4 cut(s) 221, 282, 416, 529
Cfr13I GGNCC 3 cut(s) 166, 187, 567
Cfr42I CCGCGG 1 cut(s) 562
CseI GACGC 1 cut(s) 193
Csp6I GTAC 1 cut(s) 105
CviAII CATG 2 cut(s) 111, 535
CviQI GTAC 1 cut(s) 105
DdeI CTNAG 1 cut(s) 332
DpnI GATC 3 cut(s) 330, 539, 574
DpnII GATC 3 cut(s) 328, 537, 572
EciI GGCGGA 4 cut(s) 34, 145, 223, 494
Ecl136II GAGCTC 1 cut(s) 352
Eco24I GRGCYC 1 cut(s) 354
Eco31I GGTCTC 1 cut(s) 316
Eco53kI GAGCTC 1 cut(s) 352
Eco72I CACGTG 1 cut(s) 438
Eco91I GGTNACC 1 cut(s) 514
EcoICRI GAGCTC 1 cut(s) 352
EcoNI CCTNNNNNAGG 2 cut(s) 188, 365
EcoO65I GGTNACC 1 cut(s) 514
EcoRII CCWGG 3 cut(s) 183, 189, 453
EcoT38I GRGCYC 1 cut(s) 354
Esp3I CGTCTC 2 cut(s) 211, 365
FaeI CATG 2 cut(s) 114, 538
FaiI YATR 3 cut(s) 40, 112, 536
FatI CATG 2 cut(s) 110, 534
FbaI TGATCA 1 cut(s) 537
FblI GTMKAC 2 cut(s) 324, 462
Fnu4HI GCNGC 9 cut(s) 48, 51, 195, 275, 278, 337, 417, 507, 559
FriOI GRGCYC 1 cut(s) 354
Fsp4HI GCNGC 9 cut(s) 48, 51, 195, 275, 278, 337, 417, 507, 559
FspBI CTAG 1 cut(s) 510
GlaI GCGC 4 cut(s) 220, 281, 415, 528
GluI GCNGC 9 cut(s) 48, 51, 195, 275, 278, 337, 417, 507, 559
GsaI CCCAGC 1 cut(s) 250
GsuI CTGGAG 1 cut(s) 365
HaeII RGCGCY 1 cut(s) 417
HaeIII GGCC 3 cut(s) 167, 188, 568
HapII CCGG 2 cut(s) 164, 379
HgaI GACGC 1 cut(s) 193
HhaI GCGC 4 cut(s) 221, 282, 416, 529
Hin1I GRCGYC 2 cut(s) 204, 483
Hin1II CATG 2 cut(s) 114, 538
Hin6I GCGC 4 cut(s) 219, 280, 414, 527
HinP1I GCGC 4 cut(s) 219, 280, 414, 527
HincII GTYRAC 2 cut(s) 325, 463
HindII GTYRAC 2 cut(s) 325, 463
HinfI GANTC 1 cut(s) 317
HpaII CCGG 2 cut(s) 164, 379
HphI GGTGA 1 cut(s) 484
Hpy166II GTNNAC 3 cut(s) 59, 325, 463
Hpy188I TCNGA 2 cut(s) 316, 333
Hpy188III TCNNGA 4 cut(s) 179, 424, 443, 487
Hpy8I GTNNAC 3 cut(s) 59, 325, 463
Hpy99I CGWCG 7 cut(s) 203, 326, 329, 434, 449, 467, 554
HpyAV CCTTC 3 cut(s) 41, 247, 469
HpyCH4IV ACGT 6 cut(s) 55, 107, 374, 404, 437, 483
HpyF10VI GCNNNNNNNGC 3 cut(s) 164, 194, 349
HpyF3I CTNAG 1 cut(s) 332
HpySE526I ACGT 6 cut(s) 55, 107, 374, 404, 437, 483
Hsp92I GRCGYC 2 cut(s) 204, 483
Hsp92II CATG 2 cut(s) 114, 538
HspAI GCGC 4 cut(s) 219, 280, 414, 527
Ksp22I TGATCA 1 cut(s) 537
KspI CCGCGG 1 cut(s) 562
Kzo9I GATC 3 cut(s) 328, 537, 572
LmnI GCTCC 4 cut(s) 71, 304, 349, 357
Lsp1109I GCAGC 3 cut(s) 181, 289, 493
MaeI CTAG 1 cut(s) 510
MaeII ACGT 6 cut(s) 55, 107, 374, 404, 437, 483
MaeIII GTNAC 2 cut(s) 514, 521
MalI GATC 3 cut(s) 330, 539, 574
MboI GATC 3 cut(s) 328, 537, 572
MboII GAAGA 1 cut(s) 505
MflI RGATCY 1 cut(s) 572
MhlI GDGCHC 1 cut(s) 354
MlyI GAGTC 1 cut(s) 311
MspA1I CMGCKG 1 cut(s) 561
MspI CCGG 2 cut(s) 164, 379
MspR9I CCNGG 4 cut(s) 164, 185, 191, 455
MvaI CCWGG 3 cut(s) 185, 191, 455
MvnI CGCG 5 cut(s) 180, 219, 282, 527, 561
MwoI GCNNNNNNNGC 3 cut(s) 164, 194, 349
NciI CCSGG 1 cut(s) 164
NdeII GATC 3 cut(s) 328, 537, 572
NlaIII CATG 2 cut(s) 114, 538
NlaIV GGNNCC 1 cut(s) 452
NruI TCGCGA 1 cut(s) 180
PcsI WCGNNNNNNNCGW 1 cut(s) 297
Pfl23II CGTACG 1 cut(s) 104
PflFI GACNNNGTC 1 cut(s) 320
PfoI TCCNGGA 1 cut(s) 453
PkrI GCNGC 9 cut(s) 49, 52, 196, 276, 279, 338, 418, 508, 560
PleI GAGTC 1 cut(s) 311
PmaCI CACGTG 1 cut(s) 438
PmlI CACGTG 1 cut(s) 438
PpsI GAGTC 1 cut(s) 311
Ppu21I YACGTR 2 cut(s) 56, 438
PshAI GACNNNNGTC 2 cut(s) 407, 438
Psp124BI GAGCTC 1 cut(s) 354
Psp6I CCWGG 3 cut(s) 183, 189, 453
PspCI CACGTG 1 cut(s) 438
PspEI GGTNACC 1 cut(s) 514
PspFI CCCAGC 1 cut(s) 246
PspGI CCWGG 3 cut(s) 183, 189, 453
PspLI CGTACG 1 cut(s) 104
PspN4I GGNNCC 1 cut(s) 452
PspPI GGNCC 3 cut(s) 166, 187, 567
PsuI RGATCY 1 cut(s) 572
PsyI GACNNNGTC 1 cut(s) 320
RruI TCGCGA 1 cut(s) 180
RsaI GTAC 1 cut(s) 106
RsaNI GTAC 1 cut(s) 105
SacI GAGCTC 1 cut(s) 354
SacII CCGCGG 1 cut(s) 562
SalI GTCGAC 2 cut(s) 323, 461
SatI GCNGC 9 cut(s) 48, 51, 195, 275, 278, 337, 417, 507, 559
Sau3AI GATC 3 cut(s) 328, 537, 572
Sau96I GGNCC 3 cut(s) 166, 187, 567
SchI GAGTC 1 cut(s) 311
ScrFI CCNGG 4 cut(s) 164, 185, 191, 455
SduI GDGCHC 1 cut(s) 354
Sfr303I CCGCGG 1 cut(s) 562
SgrBI CCGCGG 1 cut(s) 562
SgrDI CGTCGACG 1 cut(s) 323
SspMI CTAG 1 cut(s) 510
SstI GAGCTC 1 cut(s) 354
StyD4I CCNGG 4 cut(s) 162, 183, 189, 453
TaiI ACGT 6 cut(s) 58, 110, 377, 407, 440, 486
TaqI TCGA 4 cut(s) 324, 444, 462, 549
TauI GCSGC 6 cut(s) 50, 53, 277, 339, 419, 561
TseI GCWGC 3 cut(s) 194, 277, 506
TspGWI ACGGA 2 cut(s) 92, 310
Tth111I GACNNNGTC 1 cut(s) 320
XagI CCTNNNNNAGG 2 cut(s) 188, 365
XmiI GTMKAC 2 cut(s) 324, 462
XspI CTAG 1 cut(s) 510
ZraI GACGTC 1 cut(s) 484
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.