Rw2G007680

21 kDa protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0014269
Physical Location & Seq
Reverse (-)
7507634 .. 7508388
755 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G007680.1

Sequence Viewer

Length: 597 bp
ATGCGTTTCACTCTCCTCCCTCTCCTCCTCCTCCTCCTCCTCCTTCTCTCATACTCCGCCGCCGCACGTAAACCAAGCTCCCACAACGCCACCACCTCCTCCTCCCATCTCGTCCGTACGTCATGCCAACACGCCAGCTACCCCAACCTCTGCCTCCACACGCTCTCCGCCTCCCCGGCCCAGACCCCTCGTGACCTGGCCCAGGCTGCCGTCAGCGTCTCCCTCTCACGCTCCCGTAAAGTCTCCGCCTTCCTCTCCCAGCTCTCCCACTCCTCCTCACCGAAGCGGCAGCGCGTGGCGGTCGGCGACTGCGTGGAGCAGGTCTCGGACTCCGTCGACGAGCTGAGCCGCACGCTGGAGGAGCTCCACCACCTCCACGAGGAGACGTTCCGGTGGCAGATGAGCAACGCCCAGACGTGGGTCAGCGCCGCCCTCACGAACGACGACACGTGTCTCGACGGGTTCCAGGAGGTCGACGGCGAGGTGAAGGCGGACGTCAAGAGGAAGATTACCAATGCTGCTAGGGTTACCAGTAACGCGCTCTACATGATCAACCGGCTCGACGAGAGCCGCGGACGGCCCAGATCTAAGCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0004857 GO:0008150 GO:0030234 GO:0043086 GO:0044092 GO:0048509 GO:0050789 GO:0050790 GO:0050793 GO:0065007 GO:0065009 GO:0098772 GO:1902183
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

198

Amino Acids

21.9

Weight (kDa)

9.02

Isoelectric Point (pI)

45.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 37 - 183 9e-43 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014269)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G20740 AT5G20740
fragaria_vesca FvH4_1g09220
malus_domestica MD02G1099200.v1.1 MD15G1222000.v1.1
prunus_persica Prupe.7G193700_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0095671 RchiOBHm_Chr2g0095741
rosa_laevigata RLG00000016589
rosa_multiflora Rmu_co8375965.1_g000001
rosa_roxburghii Rroxscaffold_2G00146130
rosa_rugosa Rorug02G0053000
rosa_samantha Rh2AG101300 Rh2BG102900 Rh2CG105300 Rh2DG102700
rosa_wichuraiana Rw2G007680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 498
Acc36I ACCTGC 1 cut(s) 310
AccI GTMKAC 2 cut(s) 336, 474
AccII CGCG 3 cut(s) 294, 539, 573
AcvI CACGTG 1 cut(s) 450
AcyI GRCGYC 1 cut(s) 495
AfaI GTAC 1 cut(s) 118
AfiI CCNNNNNNNGG 5 cut(s) 202, 355, 379, 417, 418
AflIII ACRYGT 2 cut(s) 447, 449
AjiI CACGTC 1 cut(s) 417
AjnI CCWGG 3 cut(s) 195, 201, 465
AluBI AGCT 5 cut(s) 78, 138, 262, 343, 364
AluI AGCT 5 cut(s) 78, 138, 262, 343, 364
Alw21I GWGCWC 1 cut(s) 366
Alw26I GTCTC 5 cut(s) 223, 247, 328, 377, 458
AoxI GGCC 3 cut(s) 177, 198, 578
ApeKI GCWGC 3 cut(s) 206, 289, 518
AspLEI GCGC 3 cut(s) 294, 428, 541
AspS9I GGNCC 3 cut(s) 178, 199, 579
AsuC2I CCSGG 1 cut(s) 176
AsuHPI GGTGA 2 cut(s) 270, 496
BanII GRGCYC 1 cut(s) 366
BauI CACGAG 2 cut(s) 189, 377
BbrPI CACGTG 1 cut(s) 450
Bbv12I GWGCWC 1 cut(s) 366
BbvI GCAGC 3 cut(s) 193, 301, 505
BccI CCATC 1 cut(s) 114
BceAI ACGGC 3 cut(s) 194, 493, 593
BciT130I CCWGG 3 cut(s) 197, 203, 467
BclI TGATCA 1 cut(s) 549
BcnI CCSGG 1 cut(s) 176
BcoDI GTCTC 5 cut(s) 223, 247, 328, 377, 458
BfaI CTAG 1 cut(s) 522
BfoI RGCGCY 1 cut(s) 429
BfuAI ACCTGC 1 cut(s) 310
BglI GCCNNNNNGGC 1 cut(s) 176
BglII AGATCT 1 cut(s) 584
BisI GCNGC 9 cut(s) 60, 63, 207, 287, 290, 349, 429, 519, 571
BlpI GCTNAGC 1 cut(s) 344
BlsI GCNGC 9 cut(s) 61, 64, 208, 288, 291, 350, 430, 520, 572
Bme1390I CCNGG 4 cut(s) 176, 197, 203, 467
BmgBI CACGTC 1 cut(s) 417
BmgT120I GGNCC 3 cut(s) 178, 199, 579
BmiI GGNNCC 1 cut(s) 464
BmrFI CCNGG 4 cut(s) 176, 197, 203, 467
BoxI GACNNNNGTC 2 cut(s) 419, 450
BplI GAGNNNNNCTC 2 cut(s) 308, 340
BpmI CTGGAG 1 cut(s) 377
Bpu1102I GCTNAGC 1 cut(s) 344
BpuMI CCSGG 1 cut(s) 176
BsaAI YACGTR 2 cut(s) 68, 450
BsaHI GRCGYC 1 cut(s) 495
BsaI GGTCTC 1 cut(s) 328
BsaJI CCNNGG 3 cut(s) 174, 201, 571
BsaWI WCCGGW 1 cut(s) 390
BsaXI ACNNNNNCTCC 2 cut(s) 149, 179
Bsc4I CCNNNNNNNGG 5 cut(s) 202, 355, 379, 417, 418
Bse118I RCCGGY 1 cut(s) 555
Bse1I ACTGG 1 cut(s) 531
BseBI CCWGG 3 cut(s) 197, 203, 467
BseDI CCNNGG 3 cut(s) 174, 201, 571
BseLI CCNNNNNNNGG 5 cut(s) 202, 355, 379, 417, 418
BseMII CTCAG 1 cut(s) 335
BseNI ACTGG 1 cut(s) 531
BseXI GCAGC 3 cut(s) 193, 301, 505
BseYI CCCAGC 1 cut(s) 258
Bsh1236I CGCG 3 cut(s) 294, 539, 573
Bsh1285I CGRYCG 1 cut(s) 303
BshFI GGCC 3 cut(s) 179, 200, 580
BsiEI CGRYCG 1 cut(s) 303
BsiHKAI GWGCWC 1 cut(s) 366
BsiSI CCGG 3 cut(s) 176, 391, 556
BsiWI CGTACG 1 cut(s) 116
BslI CCNNNNNNNGG 5 cut(s) 202, 355, 379, 417, 418
BsmAI GTCTC 5 cut(s) 223, 247, 328, 377, 458
BsmBI CGTCTC 2 cut(s) 223, 377
BsnI GGCC 3 cut(s) 179, 200, 580
Bso31I GGTCTC 1 cut(s) 328
Bsp1286I GDGCHC 1 cut(s) 366
Bsp143I GATC 2 cut(s) 549, 584
Bsp1720I GCTNAGC 1 cut(s) 344
BspANI GGCC 3 cut(s) 179, 200, 580
BspCNI CTCAG 1 cut(s) 336
BspFNI CGCG 3 cut(s) 294, 539, 573
BspLI GGNNCC 1 cut(s) 464
BspMI ACCTGC 1 cut(s) 310
BspTNI GGTCTC 1 cut(s) 328
BsrFI RCCGGY 1 cut(s) 555
BsrI ACTGG 1 cut(s) 531
BssAI RCCGGY 1 cut(s) 555
BssECI CCNNGG 3 cut(s) 174, 201, 571
BssMI GATC 2 cut(s) 549, 584
BssNI GRCGYC 1 cut(s) 495
BssSI CACGAG 2 cut(s) 189, 377
Bst2BI CACGAG 2 cut(s) 189, 377
Bst2UI CCWGG 3 cut(s) 197, 203, 467
BstACI GRCGYC 1 cut(s) 495
BstBAI YACGTR 2 cut(s) 68, 450
BstC8I GCNNGC 2 cut(s) 136, 353
BstDEI CTNAG 2 cut(s) 344, 588
BstDSI CCRYGG 1 cut(s) 571
BstEII GGTNACC 1 cut(s) 526
BstENI CCTNNNNNAGG 2 cut(s) 200, 377
BstFNI CGCG 3 cut(s) 294, 539, 573
BstH2I RGCGCY 1 cut(s) 429
BstHHI GCGC 3 cut(s) 294, 428, 541
BstKTI GATC 2 cut(s) 552, 587
BstMAI GTCTC 5 cut(s) 223, 247, 328, 377, 458
BstMBI GATC 2 cut(s) 549, 584
BstMCI CGRYCG 1 cut(s) 303
BstMWI GCNNNNNNNGC 3 cut(s) 176, 206, 361
BstNI CCWGG 3 cut(s) 197, 203, 467
BstPAI GACNNNNGTC 2 cut(s) 419, 450
BstPI GGTNACC 1 cut(s) 526
BstSCI CCNGG 4 cut(s) 174, 195, 201, 465
BstUI CGCG 3 cut(s) 294, 539, 573
BstV1I GCAGC 3 cut(s) 193, 301, 505
BstX2I RGATCY 1 cut(s) 584
BstYI RGATCY 1 cut(s) 584
BsuRI GGCC 3 cut(s) 179, 200, 580
BtgI CCRYGG 1 cut(s) 571
BtrI CACGTC 1 cut(s) 417
BveI ACCTGC 1 cut(s) 310
Cac8I GCNNGC 2 cut(s) 136, 353
CfoI GCGC 3 cut(s) 294, 428, 541
Cfr10I RCCGGY 1 cut(s) 555
Cfr13I GGNCC 3 cut(s) 178, 199, 579
Cfr42I CCGCGG 1 cut(s) 574
CseI GACGC 1 cut(s) 205
Csp6I GTAC 1 cut(s) 117
CviAII CATG 2 cut(s) 123, 547
CviQI GTAC 1 cut(s) 117
DdeI CTNAG 2 cut(s) 344, 588
DpnI GATC 2 cut(s) 551, 586
DpnII GATC 2 cut(s) 549, 584
EciI GGCGGA 4 cut(s) 46, 157, 235, 506
Ecl136II GAGCTC 1 cut(s) 364
Eco24I GRGCYC 1 cut(s) 366
Eco31I GGTCTC 1 cut(s) 328
Eco53kI GAGCTC 1 cut(s) 364
Eco72I CACGTG 1 cut(s) 450
Eco91I GGTNACC 1 cut(s) 526
EcoICRI GAGCTC 1 cut(s) 364
EcoNI CCTNNNNNAGG 2 cut(s) 200, 377
EcoO65I GGTNACC 1 cut(s) 526
EcoRII CCWGG 3 cut(s) 195, 201, 465
EcoT38I GRGCYC 1 cut(s) 366
Esp3I CGTCTC 2 cut(s) 223, 377
FaeI CATG 2 cut(s) 126, 550
FaiI YATR 3 cut(s) 52, 124, 548
FatI CATG 2 cut(s) 122, 546
FbaI TGATCA 1 cut(s) 549
FblI GTMKAC 2 cut(s) 336, 474
Fnu4HI GCNGC 9 cut(s) 60, 63, 207, 287, 290, 349, 429, 519, 571
FriOI GRGCYC 1 cut(s) 366
Fsp4HI GCNGC 9 cut(s) 60, 63, 207, 287, 290, 349, 429, 519, 571
FspBI CTAG 1 cut(s) 522
GlaI GCGC 3 cut(s) 293, 427, 540
GluI GCNGC 9 cut(s) 60, 63, 207, 287, 290, 349, 429, 519, 571
GsaI CCCAGC 1 cut(s) 262
GsuI CTGGAG 1 cut(s) 377
HaeII RGCGCY 1 cut(s) 429
HaeIII GGCC 3 cut(s) 179, 200, 580
HapII CCGG 3 cut(s) 176, 391, 556
HgaI GACGC 1 cut(s) 205
HhaI GCGC 3 cut(s) 294, 428, 541
Hin1I GRCGYC 1 cut(s) 495
Hin1II CATG 2 cut(s) 126, 550
Hin6I GCGC 3 cut(s) 292, 426, 539
HinP1I GCGC 3 cut(s) 292, 426, 539
HincII GTYRAC 2 cut(s) 337, 475
HindII GTYRAC 2 cut(s) 337, 475
HinfI GANTC 1 cut(s) 329
HpaII CCGG 3 cut(s) 176, 391, 556
HphI GGTGA 2 cut(s) 270, 496
Hpy166II GTNNAC 3 cut(s) 71, 337, 475
Hpy188I TCNGA 1 cut(s) 328
Hpy188III TCNNGA 4 cut(s) 191, 436, 455, 499
Hpy8I GTNNAC 3 cut(s) 71, 337, 475
Hpy99I CGWCG 6 cut(s) 338, 341, 446, 461, 479, 566
HpyAV CCTTC 3 cut(s) 53, 259, 481
HpyCH4IV ACGT 6 cut(s) 67, 119, 386, 416, 449, 495
HpyF10VI GCNNNNNNNGC 3 cut(s) 176, 206, 361
HpyF3I CTNAG 2 cut(s) 344, 588
HpySE526I ACGT 6 cut(s) 67, 119, 386, 416, 449, 495
Hsp92I GRCGYC 1 cut(s) 495
Hsp92II CATG 2 cut(s) 126, 550
HspAI GCGC 3 cut(s) 292, 426, 539
Ksp22I TGATCA 1 cut(s) 549
KspI CCGCGG 1 cut(s) 574
Kzo9I GATC 2 cut(s) 549, 584
LmnI GCTCC 5 cut(s) 83, 236, 316, 361, 369
Lsp1109I GCAGC 3 cut(s) 193, 301, 505
MaeI CTAG 1 cut(s) 522
MaeII ACGT 6 cut(s) 67, 119, 386, 416, 449, 495
MaeIII GTNAC 3 cut(s) 191, 526, 533
MalI GATC 2 cut(s) 551, 586
MboI GATC 2 cut(s) 549, 584
MboII GAAGA 1 cut(s) 517
MflI RGATCY 1 cut(s) 584
MhlI GDGCHC 1 cut(s) 366
MlyI GAGTC 1 cut(s) 323
MspA1I CMGCKG 1 cut(s) 573
MspI CCGG 3 cut(s) 176, 391, 556
MspR9I CCNGG 4 cut(s) 176, 197, 203, 467
MvaI CCWGG 3 cut(s) 197, 203, 467
MvnI CGCG 3 cut(s) 294, 539, 573
MwoI GCNNNNNNNGC 3 cut(s) 176, 206, 361
NciI CCSGG 1 cut(s) 176
NdeII GATC 2 cut(s) 549, 584
NlaIII CATG 2 cut(s) 126, 550
NlaIV GGNNCC 1 cut(s) 464
NmuCI GTSAC 1 cut(s) 191
PcsI WCGNNNNNNNCGW 1 cut(s) 309
Pfl23II CGTACG 1 cut(s) 116
PflFI GACNNNGTC 1 cut(s) 332
PfoI TCCNGGA 1 cut(s) 465
PkrI GCNGC 9 cut(s) 61, 64, 208, 288, 291, 350, 430, 520, 572
PleI GAGTC 1 cut(s) 323
PmaCI CACGTG 1 cut(s) 450
PmlI CACGTG 1 cut(s) 450
PpsI GAGTC 1 cut(s) 323
Ppu21I YACGTR 2 cut(s) 68, 450
PshAI GACNNNNGTC 2 cut(s) 419, 450
Psp124BI GAGCTC 1 cut(s) 366
Psp6I CCWGG 3 cut(s) 195, 201, 465
PspCI CACGTG 1 cut(s) 450
PspEI GGTNACC 1 cut(s) 526
PspFI CCCAGC 1 cut(s) 258
PspGI CCWGG 3 cut(s) 195, 201, 465
PspLI CGTACG 1 cut(s) 116
PspN4I GGNNCC 1 cut(s) 464
PspPI GGNCC 3 cut(s) 178, 199, 579
PsuI RGATCY 1 cut(s) 584
PsyI GACNNNGTC 1 cut(s) 332
RsaI GTAC 1 cut(s) 118
RsaNI GTAC 1 cut(s) 117
SacI GAGCTC 1 cut(s) 366
SacII CCGCGG 1 cut(s) 574
SalI GTCGAC 2 cut(s) 335, 473
SatI GCNGC 9 cut(s) 60, 63, 207, 287, 290, 349, 429, 519, 571
Sau3AI GATC 2 cut(s) 549, 584
Sau96I GGNCC 3 cut(s) 178, 199, 579
SchI GAGTC 1 cut(s) 323
ScrFI CCNGG 4 cut(s) 176, 197, 203, 467
SduI GDGCHC 1 cut(s) 366
Sfr303I CCGCGG 1 cut(s) 574
SgrBI CCGCGG 1 cut(s) 574
SgrDI CGTCGACG 1 cut(s) 335
SspMI CTAG 1 cut(s) 522
SstI GAGCTC 1 cut(s) 366
StyD4I CCNGG 4 cut(s) 174, 195, 201, 465
TaiI ACGT 6 cut(s) 70, 122, 389, 419, 452, 498
TaqI TCGA 4 cut(s) 336, 456, 474, 561
TauI GCSGC 6 cut(s) 62, 65, 289, 351, 431, 573
TseFI GTSAC 1 cut(s) 191
TseI GCWGC 3 cut(s) 206, 289, 518
Tsp45I GTSAC 1 cut(s) 191
TspGWI ACGGA 2 cut(s) 104, 322
Tth111I GACNNNGTC 1 cut(s) 332
XagI CCTNNNNNAGG 2 cut(s) 200, 377
XmiI GTMKAC 2 cut(s) 336, 474
XspI CTAG 1 cut(s) 522
ZraI GACGTC 1 cut(s) 496
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.