RLG00000016589

21 kDa protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0014269
Physical Location & Seq
Reverse (-)
7991280 .. 7991873
594 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016589

Sequence Viewer

Length: 594 bp
ATGCGTTTCACTCTCCTCCCTCTCCTCCTCCTCCTTCCCTCATACTACGCCGCCGCACGTAAACTAAGCTCCCACAACGCCACCACCTCCTCCTCCCACCTCGTCCGTACGTCATGCCAACACGCCAGCTACCCCAACCTCTGCCTCCACACGCTCTCCGCCTCCCCGGCCCAGACCCCTCGCGACCTGGCCCAGGCTGCCGTCGGCGTCTCCCTCTCACGCGCCCGTAAAGTCTCCGCCTTCCTCTCCCAGCTCTCCCATTCCTCCTCCTCCTCCCCGAAGCGGCAGCGCGTGGCGGTCGGCGACTGCGTGGAGCAGGTCTCGGACTCCGTCGACGAGCTGAGCCGCACGCTGGAGGAGCTCCACCACCTCCACGAGGAGACTTTCCGGTGGCAGATGAGCAACGCCCAGACGTGGGTCAGCACCGCCCTCACCAATGACGACACGTGTCTCGACGGGTTCCAGGAGGTCGACGGCAGGGTGAAGGCGGACGTCAAGAGGAAGATTACCAATGCTGCTAGGGTTACCAGTAACGCGCTTTACATGATCAACCGGCTCGACGAGAGCCGCGGACGGCCCAGATCTAAGCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0004857 GO:0008150 GO:0030234 GO:0043086 GO:0044092 GO:0048509 GO:0050789 GO:0050790 GO:0050793 GO:0065007 GO:0065009 GO:0098772 GO:1902183
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

198

Amino Acids

21.82

Weight (kDa)

9.38

Isoelectric Point (pI)

47.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 34 - 182 3.2e-43 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014269)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G20740 AT5G20740
fragaria_vesca FvH4_1g09220
malus_domestica MD02G1099200.v1.1 MD15G1222000.v1.1
prunus_persica Prupe.7G193700_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0095671 RchiOBHm_Chr2g0095741
rosa_laevigata RLG00000016589
rosa_multiflora Rmu_co8375965.1_g000001
rosa_roxburghii Rroxscaffold_2G00146130
rosa_rugosa Rorug02G0053000
rosa_samantha Rh2AG101300 Rh2BG102900 Rh2CG105300 Rh2DG102700
rosa_wichuraiana Rw2G007680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 495
Acc36I ACCTGC 1 cut(s) 307
AccI GTMKAC 2 cut(s) 333, 471
AccII CGCG 5 cut(s) 183, 222, 291, 536, 570
AcvI CACGTG 1 cut(s) 447
AcyI GRCGYC 2 cut(s) 207, 492
AfaI GTAC 1 cut(s) 109
AfiI CCNNNNNNNGG 6 cut(s) 193, 282, 352, 376, 414, 415
AflIII ACRYGT 2 cut(s) 444, 446
AjiI CACGTC 1 cut(s) 414
AjnI CCWGG 3 cut(s) 186, 192, 462
AluBI AGCT 5 cut(s) 69, 129, 253, 340, 361
AluI AGCT 5 cut(s) 69, 129, 253, 340, 361
Alw21I GWGCWC 1 cut(s) 363
Alw26I GTCTC 5 cut(s) 214, 238, 325, 374, 455
AoxI GGCC 3 cut(s) 168, 189, 575
ApeKI GCWGC 3 cut(s) 197, 286, 515
AspLEI GCGC 3 cut(s) 224, 291, 538
AspS9I GGNCC 3 cut(s) 169, 190, 576
AsuC2I CCSGG 1 cut(s) 167
AsuHPI GGTGA 2 cut(s) 424, 493
BanII GRGCYC 1 cut(s) 363
BauI CACGAG 1 cut(s) 374
BbrPI CACGTG 1 cut(s) 447
Bbv12I GWGCWC 1 cut(s) 363
BbvI GCAGC 3 cut(s) 184, 298, 502
BceAI ACGGC 3 cut(s) 185, 490, 590
BciT130I CCWGG 3 cut(s) 188, 194, 464
BclI TGATCA 1 cut(s) 546
BcnI CCSGG 1 cut(s) 167
BcoDI GTCTC 5 cut(s) 214, 238, 325, 374, 455
BfaI CTAG 1 cut(s) 519
BfuAI ACCTGC 1 cut(s) 307
BglI GCCNNNNNGGC 1 cut(s) 167
BglII AGATCT 1 cut(s) 581
BisI GCNGC 8 cut(s) 51, 54, 198, 284, 287, 346, 516, 568
BlpI GCTNAGC 1 cut(s) 341
BlsI GCNGC 8 cut(s) 52, 55, 199, 285, 288, 347, 517, 569
Bme1390I CCNGG 4 cut(s) 167, 188, 194, 464
BmgBI CACGTC 1 cut(s) 414
BmgT120I GGNCC 3 cut(s) 169, 190, 576
BmiI GGNNCC 1 cut(s) 461
BmrFI CCNGG 4 cut(s) 167, 188, 194, 464
BoxI GACNNNNGTC 2 cut(s) 416, 447
BplI GAGNNNNNCTC 2 cut(s) 305, 337
BpmI CTGGAG 1 cut(s) 374
Bpu1102I GCTNAGC 1 cut(s) 341
BpuMI CCSGG 1 cut(s) 167
BsaAI YACGTR 2 cut(s) 59, 447
BsaHI GRCGYC 2 cut(s) 207, 492
BsaI GGTCTC 1 cut(s) 325
BsaJI CCNNGG 3 cut(s) 165, 192, 568
BsaWI WCCGGW 1 cut(s) 387
BsaXI ACNNNNNCTCC 2 cut(s) 140, 170
Bsc4I CCNNNNNNNGG 6 cut(s) 193, 282, 352, 376, 414, 415
Bse118I RCCGGY 1 cut(s) 552
Bse1I ACTGG 1 cut(s) 528
BseBI CCWGG 3 cut(s) 188, 194, 464
BseDI CCNNGG 3 cut(s) 165, 192, 568
BseLI CCNNNNNNNGG 6 cut(s) 193, 282, 352, 376, 414, 415
BseMII CTCAG 1 cut(s) 332
BseNI ACTGG 1 cut(s) 528
BseXI GCAGC 3 cut(s) 184, 298, 502
BseYI CCCAGC 1 cut(s) 249
Bsh1236I CGCG 5 cut(s) 183, 222, 291, 536, 570
Bsh1285I CGRYCG 1 cut(s) 300
BshFI GGCC 3 cut(s) 170, 191, 577
BsiEI CGRYCG 1 cut(s) 300
BsiHKAI GWGCWC 1 cut(s) 363
BsiSI CCGG 3 cut(s) 167, 388, 553
BsiWI CGTACG 1 cut(s) 107
BslI CCNNNNNNNGG 6 cut(s) 193, 282, 352, 376, 414, 415
BsmAI GTCTC 5 cut(s) 214, 238, 325, 374, 455
BsmBI CGTCTC 1 cut(s) 214
BsnI GGCC 3 cut(s) 170, 191, 577
Bso31I GGTCTC 1 cut(s) 325
Bsp1286I GDGCHC 1 cut(s) 363
Bsp143I GATC 2 cut(s) 546, 581
Bsp1720I GCTNAGC 1 cut(s) 341
Bsp68I TCGCGA 1 cut(s) 183
BspANI GGCC 3 cut(s) 170, 191, 577
BspCNI CTCAG 1 cut(s) 333
BspFNI CGCG 5 cut(s) 183, 222, 291, 536, 570
BspLI GGNNCC 1 cut(s) 461
BspMI ACCTGC 1 cut(s) 307
BspTNI GGTCTC 1 cut(s) 325
BsrFI RCCGGY 1 cut(s) 552
BsrI ACTGG 1 cut(s) 528
BssAI RCCGGY 1 cut(s) 552
BssECI CCNNGG 3 cut(s) 165, 192, 568
BssMI GATC 2 cut(s) 546, 581
BssNI GRCGYC 2 cut(s) 207, 492
BssSI CACGAG 1 cut(s) 374
Bst2BI CACGAG 1 cut(s) 374
Bst2UI CCWGG 3 cut(s) 188, 194, 464
BstACI GRCGYC 2 cut(s) 207, 492
BstBAI YACGTR 2 cut(s) 59, 447
BstC8I GCNNGC 2 cut(s) 127, 350
BstDEI CTNAG 3 cut(s) 65, 341, 585
BstDSI CCRYGG 1 cut(s) 568
BstEII GGTNACC 1 cut(s) 523
BstENI CCTNNNNNAGG 2 cut(s) 191, 374
BstFNI CGCG 5 cut(s) 183, 222, 291, 536, 570
BstHHI GCGC 3 cut(s) 224, 291, 538
BstKTI GATC 2 cut(s) 549, 584
BstMAI GTCTC 5 cut(s) 214, 238, 325, 374, 455
BstMBI GATC 2 cut(s) 546, 581
BstMCI CGRYCG 1 cut(s) 300
BstMWI GCNNNNNNNGC 3 cut(s) 167, 197, 358
BstNI CCWGG 3 cut(s) 188, 194, 464
BstPAI GACNNNNGTC 2 cut(s) 416, 447
BstPI GGTNACC 1 cut(s) 523
BstSCI CCNGG 4 cut(s) 165, 186, 192, 462
BstUI CGCG 5 cut(s) 183, 222, 291, 536, 570
BstV1I GCAGC 3 cut(s) 184, 298, 502
BstX2I RGATCY 1 cut(s) 581
BstYI RGATCY 1 cut(s) 581
BsuRI GGCC 3 cut(s) 170, 191, 577
BtgI CCRYGG 1 cut(s) 568
BtrI CACGTC 1 cut(s) 414
BtuMI TCGCGA 1 cut(s) 183
BveI ACCTGC 1 cut(s) 307
Cac8I GCNNGC 2 cut(s) 127, 350
CfoI GCGC 3 cut(s) 224, 291, 538
Cfr10I RCCGGY 1 cut(s) 552
Cfr13I GGNCC 3 cut(s) 169, 190, 576
Cfr42I CCGCGG 1 cut(s) 571
CseI GACGC 1 cut(s) 196
Csp6I GTAC 1 cut(s) 108
CviAII CATG 2 cut(s) 114, 544
CviQI GTAC 1 cut(s) 108
DdeI CTNAG 3 cut(s) 65, 341, 585
DpnI GATC 2 cut(s) 548, 583
DpnII GATC 2 cut(s) 546, 581
EciI GGCGGA 3 cut(s) 148, 226, 503
Ecl136II GAGCTC 1 cut(s) 361
Eco24I GRGCYC 1 cut(s) 363
Eco31I GGTCTC 1 cut(s) 325
Eco53kI GAGCTC 1 cut(s) 361
Eco72I CACGTG 1 cut(s) 447
Eco91I GGTNACC 1 cut(s) 523
EcoICRI GAGCTC 1 cut(s) 361
EcoNI CCTNNNNNAGG 2 cut(s) 191, 374
EcoO65I GGTNACC 1 cut(s) 523
EcoRII CCWGG 3 cut(s) 186, 192, 462
EcoT38I GRGCYC 1 cut(s) 363
Esp3I CGTCTC 1 cut(s) 214
FaeI CATG 2 cut(s) 117, 547
FaiI YATR 3 cut(s) 43, 115, 545
FatI CATG 2 cut(s) 113, 543
FbaI TGATCA 1 cut(s) 546
FblI GTMKAC 2 cut(s) 333, 471
Fnu4HI GCNGC 8 cut(s) 51, 54, 198, 284, 287, 346, 516, 568
FriOI GRGCYC 1 cut(s) 363
Fsp4HI GCNGC 8 cut(s) 51, 54, 198, 284, 287, 346, 516, 568
FspBI CTAG 1 cut(s) 519
GlaI GCGC 3 cut(s) 223, 290, 537
GluI GCNGC 8 cut(s) 51, 54, 198, 284, 287, 346, 516, 568
GsaI CCCAGC 1 cut(s) 253
GsuI CTGGAG 1 cut(s) 374
HaeIII GGCC 3 cut(s) 170, 191, 577
HapII CCGG 3 cut(s) 167, 388, 553
HgaI GACGC 1 cut(s) 196
HhaI GCGC 3 cut(s) 224, 291, 538
Hin1I GRCGYC 2 cut(s) 207, 492
Hin1II CATG 2 cut(s) 117, 547
Hin6I GCGC 3 cut(s) 222, 289, 536
HinP1I GCGC 3 cut(s) 222, 289, 536
HincII GTYRAC 2 cut(s) 334, 472
HindII GTYRAC 2 cut(s) 334, 472
HinfI GANTC 1 cut(s) 326
HpaII CCGG 3 cut(s) 167, 388, 553
HphI GGTGA 2 cut(s) 424, 493
Hpy166II GTNNAC 3 cut(s) 62, 334, 472
Hpy188I TCNGA 1 cut(s) 325
Hpy188III TCNNGA 3 cut(s) 182, 452, 496
Hpy8I GTNNAC 3 cut(s) 62, 334, 472
Hpy99I CGWCG 6 cut(s) 206, 335, 338, 458, 476, 563
HpyAV CCTTC 3 cut(s) 44, 250, 478
HpyCH4IV ACGT 5 cut(s) 58, 110, 413, 446, 492
HpyF10VI GCNNNNNNNGC 3 cut(s) 167, 197, 358
HpyF3I CTNAG 3 cut(s) 65, 341, 585
HpySE526I ACGT 5 cut(s) 58, 110, 413, 446, 492
Hsp92I GRCGYC 2 cut(s) 207, 492
Hsp92II CATG 2 cut(s) 117, 547
HspAI GCGC 3 cut(s) 222, 289, 536
Ksp22I TGATCA 1 cut(s) 546
KspI CCGCGG 1 cut(s) 571
Kzo9I GATC 2 cut(s) 546, 581
LmnI GCTCC 4 cut(s) 74, 313, 358, 366
Lsp1109I GCAGC 3 cut(s) 184, 298, 502
MaeI CTAG 1 cut(s) 519
MaeII ACGT 5 cut(s) 58, 110, 413, 446, 492
MaeIII GTNAC 2 cut(s) 523, 530
MalI GATC 2 cut(s) 548, 583
MboI GATC 2 cut(s) 546, 581
MboII GAAGA 1 cut(s) 514
MflI RGATCY 1 cut(s) 581
MhlI GDGCHC 1 cut(s) 363
MlyI GAGTC 1 cut(s) 320
MspA1I CMGCKG 1 cut(s) 570
MspI CCGG 3 cut(s) 167, 388, 553
MspR9I CCNGG 4 cut(s) 167, 188, 194, 464
MvaI CCWGG 3 cut(s) 188, 194, 464
MvnI CGCG 5 cut(s) 183, 222, 291, 536, 570
MwoI GCNNNNNNNGC 3 cut(s) 167, 197, 358
NciI CCSGG 1 cut(s) 167
NdeII GATC 2 cut(s) 546, 581
NlaIII CATG 2 cut(s) 117, 547
NlaIV GGNNCC 1 cut(s) 461
NruI TCGCGA 1 cut(s) 183
PcsI WCGNNNNNNNCGW 1 cut(s) 306
Pfl23II CGTACG 1 cut(s) 107
PflFI GACNNNGTC 1 cut(s) 329
PfoI TCCNGGA 1 cut(s) 462
PkrI GCNGC 8 cut(s) 52, 55, 199, 285, 288, 347, 517, 569
PleI GAGTC 1 cut(s) 320
PmaCI CACGTG 1 cut(s) 447
PmlI CACGTG 1 cut(s) 447
PpsI GAGTC 1 cut(s) 320
Ppu21I YACGTR 2 cut(s) 59, 447
PshAI GACNNNNGTC 2 cut(s) 416, 447
Psp124BI GAGCTC 1 cut(s) 363
Psp6I CCWGG 3 cut(s) 186, 192, 462
PspCI CACGTG 1 cut(s) 447
PspEI GGTNACC 1 cut(s) 523
PspFI CCCAGC 1 cut(s) 249
PspGI CCWGG 3 cut(s) 186, 192, 462
PspLI CGTACG 1 cut(s) 107
PspN4I GGNNCC 1 cut(s) 461
PspPI GGNCC 3 cut(s) 169, 190, 576
PsuI RGATCY 1 cut(s) 581
PsyI GACNNNGTC 1 cut(s) 329
RruI TCGCGA 1 cut(s) 183
RsaI GTAC 1 cut(s) 109
RsaNI GTAC 1 cut(s) 108
SacI GAGCTC 1 cut(s) 363
SacII CCGCGG 1 cut(s) 571
SalI GTCGAC 2 cut(s) 332, 470
SatI GCNGC 8 cut(s) 51, 54, 198, 284, 287, 346, 516, 568
Sau3AI GATC 2 cut(s) 546, 581
Sau96I GGNCC 3 cut(s) 169, 190, 576
SchI GAGTC 1 cut(s) 320
ScrFI CCNGG 4 cut(s) 167, 188, 194, 464
SduI GDGCHC 1 cut(s) 363
Sfr303I CCGCGG 1 cut(s) 571
SgrBI CCGCGG 1 cut(s) 571
SgrDI CGTCGACG 1 cut(s) 332
SspMI CTAG 1 cut(s) 519
SstI GAGCTC 1 cut(s) 363
StyD4I CCNGG 4 cut(s) 165, 186, 192, 462
TaiI ACGT 5 cut(s) 61, 113, 416, 449, 495
TaqI TCGA 4 cut(s) 333, 453, 471, 558
TauI GCSGC 5 cut(s) 53, 56, 286, 348, 570
TseI GCWGC 3 cut(s) 197, 286, 515
TspGWI ACGGA 2 cut(s) 95, 319
Tth111I GACNNNGTC 1 cut(s) 329
XagI CCTNNNNNAGG 2 cut(s) 191, 374
XmiI GTMKAC 2 cut(s) 333, 471
XspI CTAG 1 cut(s) 519
ZraI GACGTC 1 cut(s) 493
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.