Rh2CG105300

21 kDa protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0014269
Physical Location & Seq
Reverse (-)
9083949 .. 9085129
1181 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG105300.1

Sequence Viewer

Length: 600 bp
ATGCGTTTCACTCTCCTCCCTCTCCTCCTCCTCCTCCTCCTCCTTCTCTCATACTCCGCCGCCGCACGTAAACCAAGCTCCCACAACGCCACCACCTCCTCCTCCCACCTCGTCCGTACGTCATGCCAACACGCCAGCTACCCCAACCTCTGCCTCCACACGCTCTCCGCCTCCCCGGCCCAGACCCCTCGCGACCTGGCCCAGGCTGCCGTCGGCGTCTCCCTCTCACGCGCCCGTAAAGTCTCCGCCTTCCTCTCCCAGCTCTCCCATTCCTCCTCCTCCCCGAAGCGGCAGCGCGTGGCGGTCGGCGACTGCGTGGAGCAGGTCTCGGAATCCGTCGACGACCTGAGCCGCACGCTGGAGGAGCTCCACCATCTCCACGAGGAGACGTTCCGGTGGCAGATGAGCAACGCCCAGACGTGGGTCAGCACCGCCCTCACGAACGACGACACGTGTCTCGACGGGTTCCAGGAGGTCGACGGCAAGGTGAAGGCGGACGTCAAGAGGAAGATTACCAATGCTGCTAGGGTTACCAGTAACGCACTCTACATGATCAACCGGCTCGACGAGAGCCGCGGACGGCCCAGATCTAAGCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0004857 GO:0008150 GO:0030234 GO:0043086 GO:0044092 GO:0048509 GO:0050789 GO:0050790 GO:0050793 GO:0065007 GO:0065009 GO:0098772 GO:1902183
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

199

Amino Acids

21.97

Weight (kDa)

9.38

Isoelectric Point (pI)

43.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 37 - 184 3.3e-42 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014269)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G20740 AT5G20740
fragaria_vesca FvH4_1g09220
malus_domestica MD02G1099200.v1.1 MD15G1222000.v1.1
prunus_persica Prupe.7G193700_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0095671 RchiOBHm_Chr2g0095741
rosa_laevigata RLG00000016589
rosa_multiflora Rmu_co8375965.1_g000001
rosa_roxburghii Rroxscaffold_2G00146130
rosa_rugosa Rorug02G0053000
rosa_samantha Rh2AG101300 Rh2BG102900 Rh2CG105300 Rh2DG102700
rosa_wichuraiana Rw2G007680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 501
Acc36I ACCTGC 1 cut(s) 313
AccI GTMKAC 2 cut(s) 339, 477
AccII CGCG 4 cut(s) 192, 231, 297, 576
AcvI CACGTG 1 cut(s) 453
AcyI GRCGYC 2 cut(s) 216, 498
AfaI GTAC 1 cut(s) 118
AfiI CCNNNNNNNGG 5 cut(s) 202, 288, 358, 420, 421
AflIII ACRYGT 2 cut(s) 450, 452
AjiI CACGTC 1 cut(s) 420
AjnI CCWGG 3 cut(s) 195, 201, 468
AluBI AGCT 4 cut(s) 78, 138, 262, 367
AluI AGCT 4 cut(s) 78, 138, 262, 367
Alw21I GWGCWC 1 cut(s) 369
Alw26I GTCTC 5 cut(s) 223, 247, 331, 380, 461
AoxI GGCC 3 cut(s) 177, 198, 581
ApeKI GCWGC 3 cut(s) 206, 292, 521
AspLEI GCGC 2 cut(s) 233, 297
AspS9I GGNCC 3 cut(s) 178, 199, 582
AsuC2I CCSGG 1 cut(s) 176
AsuHPI GGTGA 1 cut(s) 499
BanII GRGCYC 1 cut(s) 369
BauI CACGAG 1 cut(s) 380
BbrPI CACGTG 1 cut(s) 453
Bbv12I GWGCWC 1 cut(s) 369
BbvI GCAGC 3 cut(s) 193, 304, 508
BccI CCATC 1 cut(s) 381
BceAI ACGGC 3 cut(s) 194, 496, 596
BciT130I CCWGG 3 cut(s) 197, 203, 470
BclI TGATCA 1 cut(s) 552
BcnI CCSGG 1 cut(s) 176
BcoDI GTCTC 5 cut(s) 223, 247, 331, 380, 461
BfaI CTAG 1 cut(s) 525
BfuAI ACCTGC 1 cut(s) 313
BglI GCCNNNNNGGC 1 cut(s) 176
BglII AGATCT 1 cut(s) 587
BisI GCNGC 8 cut(s) 60, 63, 207, 290, 293, 352, 522, 574
BlsI GCNGC 8 cut(s) 61, 64, 208, 291, 294, 353, 523, 575
Bme1390I CCNGG 4 cut(s) 176, 197, 203, 470
BmgBI CACGTC 1 cut(s) 420
BmgT120I GGNCC 3 cut(s) 178, 199, 582
BmiI GGNNCC 1 cut(s) 467
BmrFI CCNGG 4 cut(s) 176, 197, 203, 470
BoxI GACNNNNGTC 2 cut(s) 422, 453
BplI GAGNNNNNCTC 2 cut(s) 311, 343
BpmI CTGGAG 1 cut(s) 380
Bpu10I CCTNAGC 1 cut(s) 347
BpuMI CCSGG 1 cut(s) 176
BsaAI YACGTR 2 cut(s) 68, 453
BsaHI GRCGYC 2 cut(s) 216, 498
BsaI GGTCTC 1 cut(s) 331
BsaJI CCNNGG 3 cut(s) 174, 201, 574
BsaWI WCCGGW 1 cut(s) 393
BsaXI ACNNNNNCTCC 2 cut(s) 149, 179
Bsc4I CCNNNNNNNGG 5 cut(s) 202, 288, 358, 420, 421
Bse118I RCCGGY 1 cut(s) 558
Bse1I ACTGG 1 cut(s) 534
BseBI CCWGG 3 cut(s) 197, 203, 470
BseDI CCNNGG 3 cut(s) 174, 201, 574
BseLI CCNNNNNNNGG 5 cut(s) 202, 288, 358, 420, 421
BseMII CTCAG 1 cut(s) 338
BseNI ACTGG 1 cut(s) 534
BseXI GCAGC 3 cut(s) 193, 304, 508
BseYI CCCAGC 1 cut(s) 258
Bsh1236I CGCG 4 cut(s) 192, 231, 297, 576
Bsh1285I CGRYCG 1 cut(s) 306
BshFI GGCC 3 cut(s) 179, 200, 583
BsiEI CGRYCG 1 cut(s) 306
BsiHKAI GWGCWC 1 cut(s) 369
BsiSI CCGG 3 cut(s) 176, 394, 559
BsiWI CGTACG 1 cut(s) 116
BslI CCNNNNNNNGG 5 cut(s) 202, 288, 358, 420, 421
BsmAI GTCTC 5 cut(s) 223, 247, 331, 380, 461
BsmBI CGTCTC 2 cut(s) 223, 380
BsnI GGCC 3 cut(s) 179, 200, 583
Bso31I GGTCTC 1 cut(s) 331
Bsp1286I GDGCHC 1 cut(s) 369
Bsp143I GATC 2 cut(s) 552, 587
Bsp68I TCGCGA 1 cut(s) 192
BspANI GGCC 3 cut(s) 179, 200, 583
BspCNI CTCAG 1 cut(s) 339
BspFNI CGCG 4 cut(s) 192, 231, 297, 576
BspLI GGNNCC 1 cut(s) 467
BspMI ACCTGC 1 cut(s) 313
BspTNI GGTCTC 1 cut(s) 331
BsrFI RCCGGY 1 cut(s) 558
BsrI ACTGG 1 cut(s) 534
BssAI RCCGGY 1 cut(s) 558
BssECI CCNNGG 3 cut(s) 174, 201, 574
BssMI GATC 2 cut(s) 552, 587
BssNI GRCGYC 2 cut(s) 216, 498
BssSI CACGAG 1 cut(s) 380
Bst2BI CACGAG 1 cut(s) 380
Bst2UI CCWGG 3 cut(s) 197, 203, 470
BstACI GRCGYC 2 cut(s) 216, 498
BstBAI YACGTR 2 cut(s) 68, 453
BstC8I GCNNGC 2 cut(s) 136, 356
BstDEI CTNAG 2 cut(s) 347, 591
BstDSI CCRYGG 1 cut(s) 574
BstEII GGTNACC 1 cut(s) 529
BstENI CCTNNNNNAGG 1 cut(s) 200
BstFNI CGCG 4 cut(s) 192, 231, 297, 576
BstHHI GCGC 2 cut(s) 233, 297
BstKTI GATC 2 cut(s) 555, 590
BstMAI GTCTC 5 cut(s) 223, 247, 331, 380, 461
BstMBI GATC 2 cut(s) 552, 587
BstMCI CGRYCG 1 cut(s) 306
BstMWI GCNNNNNNNGC 3 cut(s) 176, 206, 364
BstNI CCWGG 3 cut(s) 197, 203, 470
BstPAI GACNNNNGTC 2 cut(s) 422, 453
BstPI GGTNACC 1 cut(s) 529
BstSCI CCNGG 4 cut(s) 174, 195, 201, 468
BstUI CGCG 4 cut(s) 192, 231, 297, 576
BstV1I GCAGC 3 cut(s) 193, 304, 508
BstX2I RGATCY 1 cut(s) 587
BstYI RGATCY 1 cut(s) 587
BsuRI GGCC 3 cut(s) 179, 200, 583
BtgI CCRYGG 1 cut(s) 574
BtrI CACGTC 1 cut(s) 420
BtuMI TCGCGA 1 cut(s) 192
BveI ACCTGC 1 cut(s) 313
Cac8I GCNNGC 2 cut(s) 136, 356
CfoI GCGC 2 cut(s) 233, 297
Cfr10I RCCGGY 1 cut(s) 558
Cfr13I GGNCC 3 cut(s) 178, 199, 582
Cfr42I CCGCGG 1 cut(s) 577
CseI GACGC 1 cut(s) 205
Csp6I GTAC 1 cut(s) 117
CviAII CATG 2 cut(s) 123, 550
CviQI GTAC 1 cut(s) 117
DdeI CTNAG 2 cut(s) 347, 591
DpnI GATC 2 cut(s) 554, 589
DpnII GATC 2 cut(s) 552, 587
EciI GGCGGA 4 cut(s) 46, 157, 235, 509
Ecl136II GAGCTC 1 cut(s) 367
Eco24I GRGCYC 1 cut(s) 369
Eco31I GGTCTC 1 cut(s) 331
Eco53kI GAGCTC 1 cut(s) 367
Eco72I CACGTG 1 cut(s) 453
Eco91I GGTNACC 1 cut(s) 529
EcoICRI GAGCTC 1 cut(s) 367
EcoNI CCTNNNNNAGG 1 cut(s) 200
EcoO65I GGTNACC 1 cut(s) 529
EcoRII CCWGG 3 cut(s) 195, 201, 468
EcoT38I GRGCYC 1 cut(s) 369
Esp3I CGTCTC 2 cut(s) 223, 380
FaeI CATG 2 cut(s) 126, 553
FaiI YATR 3 cut(s) 52, 124, 551
FatI CATG 2 cut(s) 122, 549
FbaI TGATCA 1 cut(s) 552
FblI GTMKAC 2 cut(s) 339, 477
Fnu4HI GCNGC 8 cut(s) 60, 63, 207, 290, 293, 352, 522, 574
FriOI GRGCYC 1 cut(s) 369
Fsp4HI GCNGC 8 cut(s) 60, 63, 207, 290, 293, 352, 522, 574
FspBI CTAG 1 cut(s) 525
GlaI GCGC 2 cut(s) 232, 296
GluI GCNGC 8 cut(s) 60, 63, 207, 290, 293, 352, 522, 574
GsaI CCCAGC 1 cut(s) 262
GsuI CTGGAG 1 cut(s) 380
HaeIII GGCC 3 cut(s) 179, 200, 583
HapII CCGG 3 cut(s) 176, 394, 559
HgaI GACGC 1 cut(s) 205
HhaI GCGC 2 cut(s) 233, 297
Hin1I GRCGYC 2 cut(s) 216, 498
Hin1II CATG 2 cut(s) 126, 553
Hin6I GCGC 2 cut(s) 231, 295
HinP1I GCGC 2 cut(s) 231, 295
HincII GTYRAC 2 cut(s) 340, 478
HindII GTYRAC 2 cut(s) 340, 478
HinfI GANTC 1 cut(s) 332
HpaII CCGG 3 cut(s) 176, 394, 559
HphI GGTGA 1 cut(s) 499
Hpy166II GTNNAC 3 cut(s) 71, 340, 478
Hpy188I TCNGA 1 cut(s) 331
Hpy188III TCNNGA 4 cut(s) 191, 439, 458, 502
Hpy8I GTNNAC 3 cut(s) 71, 340, 478
Hpy99I CGWCG 7 cut(s) 215, 341, 344, 449, 464, 482, 569
HpyAV CCTTC 3 cut(s) 53, 259, 484
HpyCH4IV ACGT 6 cut(s) 67, 119, 389, 419, 452, 498
HpyF10VI GCNNNNNNNGC 3 cut(s) 176, 206, 364
HpyF3I CTNAG 2 cut(s) 347, 591
HpySE526I ACGT 6 cut(s) 67, 119, 389, 419, 452, 498
Hsp92I GRCGYC 2 cut(s) 216, 498
Hsp92II CATG 2 cut(s) 126, 553
HspAI GCGC 2 cut(s) 231, 295
Ksp22I TGATCA 1 cut(s) 552
KspI CCGCGG 1 cut(s) 577
Kzo9I GATC 2 cut(s) 552, 587
LmnI GCTCC 4 cut(s) 83, 319, 364, 372
Lsp1109I GCAGC 3 cut(s) 193, 304, 508
MaeI CTAG 1 cut(s) 525
MaeII ACGT 6 cut(s) 67, 119, 389, 419, 452, 498
MaeIII GTNAC 2 cut(s) 529, 536
MalI GATC 2 cut(s) 554, 589
MboI GATC 2 cut(s) 552, 587
MboII GAAGA 1 cut(s) 520
MflI RGATCY 1 cut(s) 587
MhlI GDGCHC 1 cut(s) 369
MspA1I CMGCKG 1 cut(s) 576
MspI CCGG 3 cut(s) 176, 394, 559
MspR9I CCNGG 4 cut(s) 176, 197, 203, 470
MvaI CCWGG 3 cut(s) 197, 203, 470
MvnI CGCG 4 cut(s) 192, 231, 297, 576
MwoI GCNNNNNNNGC 3 cut(s) 176, 206, 364
NciI CCSGG 1 cut(s) 176
NdeII GATC 2 cut(s) 552, 587
NlaIII CATG 2 cut(s) 126, 553
NlaIV GGNNCC 1 cut(s) 467
NruI TCGCGA 1 cut(s) 192
PcsI WCGNNNNNNNCGW 1 cut(s) 312
PfeI GAWTC 1 cut(s) 332
Pfl23II CGTACG 1 cut(s) 116
PfoI TCCNGGA 1 cut(s) 468
PkrI GCNGC 8 cut(s) 61, 64, 208, 291, 294, 353, 523, 575
PmaCI CACGTG 1 cut(s) 453
PmlI CACGTG 1 cut(s) 453
Ppu21I YACGTR 2 cut(s) 68, 453
PshAI GACNNNNGTC 2 cut(s) 422, 453
Psp124BI GAGCTC 1 cut(s) 369
Psp6I CCWGG 3 cut(s) 195, 201, 468
PspCI CACGTG 1 cut(s) 453
PspEI GGTNACC 1 cut(s) 529
PspFI CCCAGC 1 cut(s) 258
PspGI CCWGG 3 cut(s) 195, 201, 468
PspLI CGTACG 1 cut(s) 116
PspN4I GGNNCC 1 cut(s) 467
PspPI GGNCC 3 cut(s) 178, 199, 582
PsuI RGATCY 1 cut(s) 587
RruI TCGCGA 1 cut(s) 192
RsaI GTAC 1 cut(s) 118
RsaNI GTAC 1 cut(s) 117
SacI GAGCTC 1 cut(s) 369
SacII CCGCGG 1 cut(s) 577
SalI GTCGAC 2 cut(s) 338, 476
SatI GCNGC 8 cut(s) 60, 63, 207, 290, 293, 352, 522, 574
Sau3AI GATC 2 cut(s) 552, 587
Sau96I GGNCC 3 cut(s) 178, 199, 582
ScrFI CCNGG 4 cut(s) 176, 197, 203, 470
SduI GDGCHC 1 cut(s) 369
Sfr303I CCGCGG 1 cut(s) 577
SgrBI CCGCGG 1 cut(s) 577
SgrDI CGTCGACG 1 cut(s) 338
SspMI CTAG 1 cut(s) 525
SstI GAGCTC 1 cut(s) 369
StyD4I CCNGG 4 cut(s) 174, 195, 201, 468
TaiI ACGT 6 cut(s) 70, 122, 392, 422, 455, 501
TaqI TCGA 4 cut(s) 339, 459, 477, 564
TauI GCSGC 5 cut(s) 62, 65, 292, 354, 576
TfiI GAWTC 1 cut(s) 332
TseI GCWGC 3 cut(s) 206, 292, 521
TspGWI ACGGA 2 cut(s) 104, 325
XagI CCTNNNNNAGG 1 cut(s) 200
XmiI GTMKAC 2 cut(s) 339, 477
XspI CTAG 1 cut(s) 525
ZraI GACGTC 1 cut(s) 499
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.