Rroxscaffold_2G00146130

21 kDa protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0014269
Physical Location & Seq
Forward (+)
84018852 .. 84019461
610 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00146130.1

Sequence Viewer

Length: 540 bp
ATGCGTTTCACTCTCCTCCCCCCTCCTCCTCCTCCTTCTCTCATACTCTGCCGCCGCACCTACCCCATCCTCTGCCTCCACACGCTCTCCGCCTCCCCGGCCCAGACCCCTCGCGACCTGGCCCAGGCTGCCGTCGGCGTCTCCCTCTCACGCGCCCGTAAAGTCTCCGCCTTCCTCTCCCAGCTCTCCCATTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCCCGAAGCGGCAGCGCGTGGCGGTCGGCGACTGCGTGGAGCAGGTCTCGGACTCCGTCGACGAGCTGAGCCGCACGCTGGAGGAGCTCCACCACCTCCACGAGGAGACGTTCCGGTGGCAGATGAGCAACGCCCAGACGTGGGTCAGCGCCGCCCTCACGAACGACGACACGTGTCTCGACGGGTTCCAGGAGGTCGACGGCGAGGTGAAGGCGGACGTCAAGAGGAAGATTACCAATGCTGCTAGGGTTACCAGTAACGCGCTCTACATGATCAACCGGCTCGACGAGAGCCGCGGACGGCCCAGATCTAAGCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0004857 GO:0008150 GO:0030234 GO:0043086 GO:0044092 GO:0048509 GO:0050789 GO:0050790 GO:0050793 GO:0065007 GO:0065009 GO:0098772 GO:1902183
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

179

Amino Acids

19.76

Weight (kDa)

8.8

Isoelectric Point (pI)

58.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 16 - 164 2.9e-40 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014269)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G20740 AT5G20740
fragaria_vesca FvH4_1g09220
malus_domestica MD02G1099200.v1.1 MD15G1222000.v1.1
prunus_persica Prupe.7G193700_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0095671 RchiOBHm_Chr2g0095741
rosa_laevigata RLG00000016589
rosa_multiflora Rmu_co8375965.1_g000001
rosa_roxburghii Rroxscaffold_2G00146130
rosa_rugosa Rorug02G0053000
rosa_samantha Rh2AG101300 Rh2BG102900 Rh2CG105300 Rh2DG102700
rosa_wichuraiana Rw2G007680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 441
Acc36I ACCTGC 1 cut(s) 253
AccI GTMKAC 2 cut(s) 279, 417
AccII CGCG 5 cut(s) 114, 153, 237, 482, 516
AcvI CACGTG 1 cut(s) 393
AcyI GRCGYC 2 cut(s) 138, 438
AfiI CCNNNNNNNGG 6 cut(s) 124, 228, 298, 322, 360, 361
AflIII ACRYGT 2 cut(s) 390, 392
AjiI CACGTC 1 cut(s) 360
AjnI CCWGG 3 cut(s) 117, 123, 408
AluBI AGCT 3 cut(s) 184, 286, 307
AluI AGCT 3 cut(s) 184, 286, 307
Alw21I GWGCWC 1 cut(s) 309
Alw26I GTCTC 5 cut(s) 145, 169, 271, 320, 401
AoxI GGCC 3 cut(s) 99, 120, 521
ApeKI GCWGC 3 cut(s) 128, 232, 461
AspLEI GCGC 4 cut(s) 155, 237, 371, 484
AspS9I GGNCC 3 cut(s) 100, 121, 522
AsuC2I CCSGG 1 cut(s) 98
AsuHPI GGTGA 1 cut(s) 439
BanII GRGCYC 1 cut(s) 309
BauI CACGAG 1 cut(s) 320
BbrPI CACGTG 1 cut(s) 393
Bbv12I GWGCWC 1 cut(s) 309
BbvI GCAGC 3 cut(s) 115, 244, 448
BccI CCATC 1 cut(s) 74
BceAI ACGGC 3 cut(s) 116, 436, 536
BciT130I CCWGG 3 cut(s) 119, 125, 410
BclI TGATCA 1 cut(s) 492
BcnI CCSGG 1 cut(s) 98
BcoDI GTCTC 5 cut(s) 145, 169, 271, 320, 401
BfaI CTAG 1 cut(s) 465
BfoI RGCGCY 1 cut(s) 372
BfuAI ACCTGC 1 cut(s) 253
BglI GCCNNNNNGGC 1 cut(s) 98
BglII AGATCT 1 cut(s) 527
BisI GCNGC 9 cut(s) 52, 55, 129, 230, 233, 292, 372, 462, 514
BlpI GCTNAGC 1 cut(s) 287
BlsI GCNGC 9 cut(s) 53, 56, 130, 231, 234, 293, 373, 463, 515
Bme1390I CCNGG 4 cut(s) 98, 119, 125, 410
BmgBI CACGTC 1 cut(s) 360
BmgT120I GGNCC 3 cut(s) 100, 121, 522
BmiI GGNNCC 1 cut(s) 407
BmrFI CCNGG 4 cut(s) 98, 119, 125, 410
BoxI GACNNNNGTC 2 cut(s) 362, 393
BplI GAGNNNNNCTC 2 cut(s) 251, 283
BpmI CTGGAG 1 cut(s) 320
Bpu1102I GCTNAGC 1 cut(s) 287
BpuMI CCSGG 1 cut(s) 98
BsaAI YACGTR 1 cut(s) 393
BsaHI GRCGYC 2 cut(s) 138, 438
BsaI GGTCTC 1 cut(s) 271
BsaJI CCNNGG 3 cut(s) 96, 123, 514
BsaWI WCCGGW 1 cut(s) 333
BsaXI ACNNNNNCTCC 2 cut(s) 71, 101
Bsc4I CCNNNNNNNGG 6 cut(s) 124, 228, 298, 322, 360, 361
Bse118I RCCGGY 1 cut(s) 498
Bse1I ACTGG 1 cut(s) 474
BseBI CCWGG 3 cut(s) 119, 125, 410
BseDI CCNNGG 3 cut(s) 96, 123, 514
BseGI GGATG 1 cut(s) 66
BseLI CCNNNNNNNGG 6 cut(s) 124, 228, 298, 322, 360, 361
BseMII CTCAG 1 cut(s) 278
BseNI ACTGG 1 cut(s) 474
BseXI GCAGC 3 cut(s) 115, 244, 448
BseYI CCCAGC 1 cut(s) 180
Bsh1236I CGCG 5 cut(s) 114, 153, 237, 482, 516
Bsh1285I CGRYCG 1 cut(s) 246
BshFI GGCC 3 cut(s) 101, 122, 523
BsiEI CGRYCG 1 cut(s) 246
BsiHKAI GWGCWC 1 cut(s) 309
BsiSI CCGG 3 cut(s) 98, 334, 499
BslI CCNNNNNNNGG 6 cut(s) 124, 228, 298, 322, 360, 361
BsmAI GTCTC 5 cut(s) 145, 169, 271, 320, 401
BsmBI CGTCTC 2 cut(s) 145, 320
BsnI GGCC 3 cut(s) 101, 122, 523
Bso31I GGTCTC 1 cut(s) 271
Bsp1286I GDGCHC 1 cut(s) 309
Bsp143I GATC 2 cut(s) 492, 527
Bsp1720I GCTNAGC 1 cut(s) 287
Bsp68I TCGCGA 1 cut(s) 114
BspANI GGCC 3 cut(s) 101, 122, 523
BspCNI CTCAG 1 cut(s) 279
BspFNI CGCG 5 cut(s) 114, 153, 237, 482, 516
BspLI GGNNCC 1 cut(s) 407
BspMI ACCTGC 1 cut(s) 253
BspTNI GGTCTC 1 cut(s) 271
BsrFI RCCGGY 1 cut(s) 498
BsrI ACTGG 1 cut(s) 474
BssAI RCCGGY 1 cut(s) 498
BssECI CCNNGG 3 cut(s) 96, 123, 514
BssMI GATC 2 cut(s) 492, 527
BssNI GRCGYC 2 cut(s) 138, 438
BssSI CACGAG 1 cut(s) 320
Bst2BI CACGAG 1 cut(s) 320
Bst2UI CCWGG 3 cut(s) 119, 125, 410
BstACI GRCGYC 2 cut(s) 138, 438
BstBAI YACGTR 1 cut(s) 393
BstC8I GCNNGC 1 cut(s) 296
BstDEI CTNAG 2 cut(s) 287, 531
BstDSI CCRYGG 1 cut(s) 514
BstEII GGTNACC 1 cut(s) 469
BstENI CCTNNNNNAGG 2 cut(s) 122, 320
BstF5I GGATG 1 cut(s) 66
BstFNI CGCG 5 cut(s) 114, 153, 237, 482, 516
BstH2I RGCGCY 1 cut(s) 372
BstHHI GCGC 4 cut(s) 155, 237, 371, 484
BstKTI GATC 2 cut(s) 495, 530
BstMAI GTCTC 5 cut(s) 145, 169, 271, 320, 401
BstMBI GATC 2 cut(s) 492, 527
BstMCI CGRYCG 1 cut(s) 246
BstMWI GCNNNNNNNGC 3 cut(s) 98, 128, 304
BstNI CCWGG 3 cut(s) 119, 125, 410
BstPAI GACNNNNGTC 2 cut(s) 362, 393
BstPI GGTNACC 1 cut(s) 469
BstSCI CCNGG 4 cut(s) 96, 117, 123, 408
BstUI CGCG 5 cut(s) 114, 153, 237, 482, 516
BstV1I GCAGC 3 cut(s) 115, 244, 448
BstX2I RGATCY 1 cut(s) 527
BstYI RGATCY 1 cut(s) 527
BsuRI GGCC 3 cut(s) 101, 122, 523
BtgI CCRYGG 1 cut(s) 514
BtrI CACGTC 1 cut(s) 360
BtsCI GGATG 1 cut(s) 66
BtuMI TCGCGA 1 cut(s) 114
BveI ACCTGC 1 cut(s) 253
Cac8I GCNNGC 1 cut(s) 296
CfoI GCGC 4 cut(s) 155, 237, 371, 484
Cfr10I RCCGGY 1 cut(s) 498
Cfr13I GGNCC 3 cut(s) 100, 121, 522
Cfr42I CCGCGG 1 cut(s) 517
CseI GACGC 1 cut(s) 127
CviAII CATG 1 cut(s) 490
DdeI CTNAG 2 cut(s) 287, 531
DpnI GATC 2 cut(s) 494, 529
DpnII GATC 2 cut(s) 492, 527
EciI GGCGGA 3 cut(s) 79, 157, 449
Ecl136II GAGCTC 1 cut(s) 307
Eco24I GRGCYC 1 cut(s) 309
Eco31I GGTCTC 1 cut(s) 271
Eco53kI GAGCTC 1 cut(s) 307
Eco72I CACGTG 1 cut(s) 393
Eco91I GGTNACC 1 cut(s) 469
EcoICRI GAGCTC 1 cut(s) 307
EcoNI CCTNNNNNAGG 2 cut(s) 122, 320
EcoO65I GGTNACC 1 cut(s) 469
EcoRII CCWGG 3 cut(s) 117, 123, 408
EcoT38I GRGCYC 1 cut(s) 309
Esp3I CGTCTC 2 cut(s) 145, 320
FaeI CATG 1 cut(s) 493
FaiI YATR 2 cut(s) 44, 491
FatI CATG 1 cut(s) 489
FbaI TGATCA 1 cut(s) 492
FblI GTMKAC 2 cut(s) 279, 417
Fnu4HI GCNGC 9 cut(s) 52, 55, 129, 230, 233, 292, 372, 462, 514
FokI GGATG 1 cut(s) 53
FriOI GRGCYC 1 cut(s) 309
Fsp4HI GCNGC 9 cut(s) 52, 55, 129, 230, 233, 292, 372, 462, 514
FspBI CTAG 1 cut(s) 465
GlaI GCGC 4 cut(s) 154, 236, 370, 483
GluI GCNGC 9 cut(s) 52, 55, 129, 230, 233, 292, 372, 462, 514
GsaI CCCAGC 1 cut(s) 184
GsuI CTGGAG 1 cut(s) 320
HaeII RGCGCY 1 cut(s) 372
HaeIII GGCC 3 cut(s) 101, 122, 523
HapII CCGG 3 cut(s) 98, 334, 499
HgaI GACGC 1 cut(s) 127
HhaI GCGC 4 cut(s) 155, 237, 371, 484
Hin1I GRCGYC 2 cut(s) 138, 438
Hin1II CATG 1 cut(s) 493
Hin6I GCGC 4 cut(s) 153, 235, 369, 482
HinP1I GCGC 4 cut(s) 153, 235, 369, 482
HincII GTYRAC 2 cut(s) 280, 418
HindII GTYRAC 2 cut(s) 280, 418
HinfI GANTC 1 cut(s) 272
HpaII CCGG 3 cut(s) 98, 334, 499
HphI GGTGA 1 cut(s) 439
Hpy166II GTNNAC 2 cut(s) 280, 418
Hpy188I TCNGA 1 cut(s) 271
Hpy188III TCNNGA 4 cut(s) 113, 379, 398, 442
Hpy8I GTNNAC 2 cut(s) 280, 418
Hpy99I CGWCG 7 cut(s) 137, 281, 284, 389, 404, 422, 509
HpyAV CCTTC 3 cut(s) 45, 181, 424
HpyCH4IV ACGT 4 cut(s) 329, 359, 392, 438
HpyF10VI GCNNNNNNNGC 3 cut(s) 98, 128, 304
HpyF3I CTNAG 2 cut(s) 287, 531
HpySE526I ACGT 4 cut(s) 329, 359, 392, 438
Hsp92I GRCGYC 2 cut(s) 138, 438
Hsp92II CATG 1 cut(s) 493
HspAI GCGC 4 cut(s) 153, 235, 369, 482
Ksp22I TGATCA 1 cut(s) 492
KspI CCGCGG 1 cut(s) 517
Kzo9I GATC 2 cut(s) 492, 527
LmnI GCTCC 3 cut(s) 259, 304, 312
Lsp1109I GCAGC 3 cut(s) 115, 244, 448
MaeI CTAG 1 cut(s) 465
MaeII ACGT 4 cut(s) 329, 359, 392, 438
MaeIII GTNAC 2 cut(s) 469, 476
MalI GATC 2 cut(s) 494, 529
MboI GATC 2 cut(s) 492, 527
MboII GAAGA 1 cut(s) 460
MflI RGATCY 1 cut(s) 527
MhlI GDGCHC 1 cut(s) 309
MlyI GAGTC 1 cut(s) 266
MspA1I CMGCKG 1 cut(s) 516
MspI CCGG 3 cut(s) 98, 334, 499
MspR9I CCNGG 4 cut(s) 98, 119, 125, 410
MvaI CCWGG 3 cut(s) 119, 125, 410
MvnI CGCG 5 cut(s) 114, 153, 237, 482, 516
MwoI GCNNNNNNNGC 3 cut(s) 98, 128, 304
NciI CCSGG 1 cut(s) 98
NdeII GATC 2 cut(s) 492, 527
NlaIII CATG 1 cut(s) 493
NlaIV GGNNCC 1 cut(s) 407
NruI TCGCGA 1 cut(s) 114
PcsI WCGNNNNNNNCGW 1 cut(s) 252
PflFI GACNNNGTC 1 cut(s) 275
PfoI TCCNGGA 1 cut(s) 408
PkrI GCNGC 9 cut(s) 53, 56, 130, 231, 234, 293, 373, 463, 515
PleI GAGTC 1 cut(s) 266
PmaCI CACGTG 1 cut(s) 393
PmlI CACGTG 1 cut(s) 393
PpsI GAGTC 1 cut(s) 266
Ppu21I YACGTR 1 cut(s) 393
PshAI GACNNNNGTC 2 cut(s) 362, 393
Psp124BI GAGCTC 1 cut(s) 309
Psp6I CCWGG 3 cut(s) 117, 123, 408
PspCI CACGTG 1 cut(s) 393
PspEI GGTNACC 1 cut(s) 469
PspFI CCCAGC 1 cut(s) 180
PspGI CCWGG 3 cut(s) 117, 123, 408
PspN4I GGNNCC 1 cut(s) 407
PspPI GGNCC 3 cut(s) 100, 121, 522
PsuI RGATCY 1 cut(s) 527
PsyI GACNNNGTC 1 cut(s) 275
RruI TCGCGA 1 cut(s) 114
SacI GAGCTC 1 cut(s) 309
SacII CCGCGG 1 cut(s) 517
SalI GTCGAC 2 cut(s) 278, 416
SatI GCNGC 9 cut(s) 52, 55, 129, 230, 233, 292, 372, 462, 514
Sau3AI GATC 2 cut(s) 492, 527
Sau96I GGNCC 3 cut(s) 100, 121, 522
SchI GAGTC 1 cut(s) 266
ScrFI CCNGG 4 cut(s) 98, 119, 125, 410
SduI GDGCHC 1 cut(s) 309
Sfr303I CCGCGG 1 cut(s) 517
SgrBI CCGCGG 1 cut(s) 517
SgrDI CGTCGACG 1 cut(s) 278
SspMI CTAG 1 cut(s) 465
SstI GAGCTC 1 cut(s) 309
StyD4I CCNGG 4 cut(s) 96, 117, 123, 408
TaiI ACGT 4 cut(s) 332, 362, 395, 441
TaqI TCGA 4 cut(s) 279, 399, 417, 504
TauI GCSGC 6 cut(s) 54, 57, 232, 294, 374, 516
TseI GCWGC 3 cut(s) 128, 232, 461
TspGWI ACGGA 1 cut(s) 265
Tth111I GACNNNGTC 1 cut(s) 275
XagI CCTNNNNNAGG 2 cut(s) 122, 320
XmiI GTMKAC 2 cut(s) 279, 417
XspI CTAG 1 cut(s) 465
ZraI GACGTC 1 cut(s) 439
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.