MD02G1099200.v1.1

21 kDa protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0014269
Physical Location & Seq
Reverse (-)
7862679 .. 7863897
1219 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1099200.v1.1.491

Sequence Viewer

Length: 618 bp
ATGCGTTTCCTGCTCTCTCTTCTCCCTCTCCTCGTCCTCCTCCTCTGCTCGCCTTACTCCGCCGCCGCCGCCGCCGCCGCACAGAAACACACTCCACAGCACAACCCAGCATCAACTCTCGTCCGTTCATCATGTCGCCACGCCACCTACCCCAAAATCTGCCTCCGCACACTCTCCGACTACGCCGGCCCCGCCAAGACCCCCCGCGACCTCGCCCAGGCGTCCGTGAGCGTCAGCCTTGCCCGCGCCCGCCGCGTCTCGGGCTTCCTCGCCCAGCTCTCCGCCAACTTCCAGGGGACAAAGAGGCAGCGCTCGGCGATAGGCGACTGCGTGGACCTGATGTCGTCGTCGGTGGACGAGCTGAGCGAGACGCTCGGCGAGCTGCAGCACCTCCGGGAGGAGACGTTTCTATTCCAGATGAGCAACGCCCAGACGTGGCTCAGCGCCGCCCTCACGGACGACGACACGTGCCTGGACGGGATTCAGGAGGCGGATGTGAAGGCCAAGGCCGCGGATGTGAAGCGGAAGATTAAGAATGCGGCTAGGGTTACGAGCAACGCGCTGTACCTGATCAATCGCCTTGACGAGAGCCGCGGACGGCCCAGATCTAGACCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0004857 GO:0008150 GO:0030234 GO:0043086 GO:0044092 GO:0048509 GO:0050789 GO:0050790 GO:0050793 GO:0065007 GO:0065009 GO:0098772 GO:1902183
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

206

Amino Acids

22.38

Weight (kDa)

9.5

Isoelectric Point (pI)

46.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 40 - 190 6.3e-38 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014269)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G20740 AT5G20740
fragaria_vesca FvH4_1g09220
malus_domestica MD02G1099200.v1.1 MD15G1222000.v1.1
prunus_persica Prupe.7G193700_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0095671 RchiOBHm_Chr2g0095741
rosa_laevigata RLG00000016589
rosa_multiflora Rmu_co8375965.1_g000001
rosa_roxburghii Rroxscaffold_2G00146130
rosa_rugosa Rorug02G0053000
rosa_samantha Rh2AG101300 Rh2BG102900 Rh2CG105300 Rh2DG102700
rosa_wichuraiana Rw2G007680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 6 cut(s) 207, 246, 255, 512, 560, 594
AcvI CACGTG 1 cut(s) 468
AcyI GRCGYC 1 cut(s) 221
AfaI GTAC 1 cut(s) 566
AfeI AGCGCT 1 cut(s) 311
AfiI CCNNNNNNNGG 4 cut(s) 217, 259, 397, 435
AflIII ACRYGT 1 cut(s) 465
AhdI GACNNNNNGTC 1 cut(s) 340
AjiI CACGTC 1 cut(s) 435
AjnI CCWGG 3 cut(s) 216, 291, 471
AluBI AGCT 3 cut(s) 277, 361, 382
AluI AGCT 3 cut(s) 277, 361, 382
Alw26I GTCTC 3 cut(s) 262, 362, 395
Ama87I CYCGRG 1 cut(s) 259
Aor51HI AGCGCT 1 cut(s) 311
AoxI GGCC 4 cut(s) 187, 501, 507, 599
ApeKI GCWGC 3 cut(s) 307, 382, 385
AspLEI GCGC 4 cut(s) 248, 312, 446, 562
AspS9I GGNCC 3 cut(s) 188, 334, 600
AsuC2I CCSGG 1 cut(s) 395
AvaI CYCGRG 1 cut(s) 259
AvaII GGWCC 1 cut(s) 334
BbrPI CACGTG 1 cut(s) 468
BbvI GCAGC 3 cut(s) 319, 369, 397
BceAI ACGGC 1 cut(s) 614
BciT130I CCWGG 3 cut(s) 218, 293, 473
BclI TGATCA 1 cut(s) 570
BcnI CCSGG 1 cut(s) 395
BcoDI GTCTC 3 cut(s) 262, 362, 395
BfaI CTAG 2 cut(s) 543, 609
BfmI CTRYAG 1 cut(s) 383
BfoI RGCGCY 2 cut(s) 313, 447
BglII AGATCT 1 cut(s) 605
BlpI GCTNAGC 2 cut(s) 362, 440
Bme1390I CCNGG 4 cut(s) 218, 293, 395, 473
Bme18I GGWCC 1 cut(s) 334
BmeRI GACNNNNNGTC 1 cut(s) 340
BmeT110I CYCGRG 1 cut(s) 259
BmgBI CACGTC 1 cut(s) 435
BmgT120I GGNCC 3 cut(s) 188, 334, 600
BmiI GGNNCC 1 cut(s) 190
BmrFI CCNGG 4 cut(s) 218, 293, 395, 473
BmsI GCATC 1 cut(s) 119
Bpu1102I GCTNAGC 2 cut(s) 362, 440
BpuMI CCSGG 1 cut(s) 395
BsaAI YACGTR 1 cut(s) 468
BsaHI GRCGYC 1 cut(s) 221
BsaJI CCNNGG 5 cut(s) 216, 292, 504, 510, 592
BsaXI ACNNNNNCTCC 2 cut(s) 389, 419
Bsc4I CCNNNNNNNGG 4 cut(s) 217, 259, 397, 435
Bse118I RCCGGY 1 cut(s) 185
BseBI CCWGG 3 cut(s) 218, 293, 473
BseDI CCNNGG 5 cut(s) 216, 292, 504, 510, 592
BseGI GGATG 2 cut(s) 499, 520
BseLI CCNNNNNNNGG 4 cut(s) 217, 259, 397, 435
BseMII CTCAG 2 cut(s) 353, 454
BseRI GAGGAG 4 cut(s) 20, 29, 32, 413
BseXI GCAGC 3 cut(s) 319, 369, 397
BseYI CCCAGC 2 cut(s) 106, 273
Bsh1236I CGCG 6 cut(s) 207, 246, 255, 512, 560, 594
BshFI GGCC 4 cut(s) 189, 503, 509, 601
BsiHKCI CYCGRG 1 cut(s) 259
BsiSI CCGG 2 cut(s) 186, 394
BslFI GGGAC 1 cut(s) 310
BslI CCNNNNNNNGG 4 cut(s) 217, 259, 397, 435
BsmAI GTCTC 3 cut(s) 262, 362, 395
BsmBI CGTCTC 3 cut(s) 262, 362, 395
BsmFI GGGAC 1 cut(s) 310
BsmI GAATGC 1 cut(s) 541
BsnI GGCC 4 cut(s) 189, 503, 509, 601
BsoBI CYCGRG 1 cut(s) 259
Bsp143I GATC 2 cut(s) 570, 605
Bsp1720I GCTNAGC 2 cut(s) 362, 440
BspANI GGCC 4 cut(s) 189, 503, 509, 601
BspCNI CTCAG 2 cut(s) 354, 453
BspFNI CGCG 6 cut(s) 207, 246, 255, 512, 560, 594
BspLI GGNNCC 1 cut(s) 190
BspMAI CTGCAG 1 cut(s) 387
BsrFI RCCGGY 1 cut(s) 185
BssAI RCCGGY 1 cut(s) 185
BssECI CCNNGG 5 cut(s) 216, 292, 504, 510, 592
BssMI GATC 2 cut(s) 570, 605
BssNI GRCGYC 1 cut(s) 221
BssT1I CCWWGG 1 cut(s) 504
Bst2UI CCWGG 3 cut(s) 218, 293, 473
Bst6I CTCTTC 1 cut(s) 24
BstACI GRCGYC 1 cut(s) 221
BstBAI YACGTR 1 cut(s) 468
BstC8I GCNNGC 5 cut(s) 50, 187, 244, 250, 380
BstDEI CTNAG 2 cut(s) 362, 440
BstDSI CCRYGG 2 cut(s) 510, 592
BstENI CCTNNNNNAGG 2 cut(s) 215, 395
BstF5I GGATG 2 cut(s) 499, 520
BstFNI CGCG 6 cut(s) 207, 246, 255, 512, 560, 594
BstH2I RGCGCY 2 cut(s) 313, 447
BstHHI GCGC 4 cut(s) 248, 312, 446, 562
BstKTI GATC 2 cut(s) 573, 608
BstMAI GTCTC 3 cut(s) 262, 362, 395
BstMBI GATC 2 cut(s) 570, 605
BstNI CCWGG 3 cut(s) 218, 293, 473
BstSCI CCNGG 4 cut(s) 216, 291, 393, 471
BstSFI CTRYAG 1 cut(s) 383
BstUI CGCG 6 cut(s) 207, 246, 255, 512, 560, 594
BstV1I GCAGC 3 cut(s) 319, 369, 397
BstX2I RGATCY 1 cut(s) 605
BstYI RGATCY 1 cut(s) 605
BsuRI GGCC 4 cut(s) 189, 503, 509, 601
BtgI CCRYGG 2 cut(s) 510, 592
BtrI CACGTC 1 cut(s) 435
BtsCI GGATG 2 cut(s) 499, 520
Cac8I GCNNGC 5 cut(s) 50, 187, 244, 250, 380
CfoI GCGC 4 cut(s) 248, 312, 446, 562
Cfr10I RCCGGY 1 cut(s) 185
Cfr13I GGNCC 3 cut(s) 188, 334, 600
Cfr42I CCGCGG 2 cut(s) 513, 595
CseI GACGC 4 cut(s) 210, 220, 244, 379
Csp6I GTAC 1 cut(s) 565
CviAII CATG 1 cut(s) 132
CviQI GTAC 1 cut(s) 565
DdeI CTNAG 2 cut(s) 362, 440
DpnI GATC 2 cut(s) 572, 607
DpnII GATC 2 cut(s) 570, 605
DriI GACNNNNNGTC 1 cut(s) 340
Eam1104I CTCTTC 1 cut(s) 24
Eam1105I GACNNNNNGTC 1 cut(s) 340
EarI CTCTTC 1 cut(s) 24
EciI GGCGGA 3 cut(s) 49, 271, 506
Eco130I CCWWGG 1 cut(s) 504
Eco47I GGWCC 1 cut(s) 334
Eco47III AGCGCT 1 cut(s) 311
Eco72I CACGTG 1 cut(s) 468
Eco88I CYCGRG 1 cut(s) 259
EcoNI CCTNNNNNAGG 2 cut(s) 215, 395
EcoRII CCWGG 3 cut(s) 216, 291, 471
EcoT14I CCWWGG 1 cut(s) 504
ErhI CCWWGG 1 cut(s) 504
Esp3I CGTCTC 3 cut(s) 262, 362, 395
FaeI CATG 1 cut(s) 135
FaiI YATR 1 cut(s) 133
FaqI GGGAC 1 cut(s) 310
FatI CATG 1 cut(s) 131
FauI CCCGC 4 cut(s) 199, 212, 251, 257
FbaI TGATCA 1 cut(s) 570
FokI GGATG 2 cut(s) 506, 527
FspBI CTAG 2 cut(s) 543, 609
GlaI GCGC 4 cut(s) 247, 311, 445, 561
GsaI CCCAGC 2 cut(s) 110, 277
HaeII RGCGCY 2 cut(s) 313, 447
HaeIII GGCC 4 cut(s) 189, 503, 509, 601
HapII CCGG 2 cut(s) 186, 394
HgaI GACGC 4 cut(s) 210, 220, 244, 379
HhaI GCGC 4 cut(s) 248, 312, 446, 562
Hin1I GRCGYC 1 cut(s) 221
Hin1II CATG 1 cut(s) 135
Hin6I GCGC 4 cut(s) 246, 310, 444, 560
HinP1I GCGC 4 cut(s) 246, 310, 444, 560
HinfI GANTC 1 cut(s) 481
HpaII CCGG 2 cut(s) 186, 394
Hpy166II GTNNAC 2 cut(s) 334, 355
Hpy188I TCNGA 1 cut(s) 178
Hpy188III TCNNGA 3 cut(s) 415, 485, 609
Hpy8I GTNNAC 2 cut(s) 334, 355
Hpy99I CGWCG 3 cut(s) 349, 352, 464
HpyAV CCTTC 1 cut(s) 493
HpyCH4IV ACGT 3 cut(s) 404, 434, 467
HpyCH4V TGCA 1 cut(s) 385
HpyF3I CTNAG 2 cut(s) 362, 440
HpySE526I ACGT 3 cut(s) 404, 434, 467
Hsp92I GRCGYC 1 cut(s) 221
Hsp92II CATG 1 cut(s) 135
HspAI GCGC 4 cut(s) 246, 310, 444, 560
KroI GCCGGC 1 cut(s) 185
KroNI GCCGGC 1 cut(s) 187
Ksp22I TGATCA 1 cut(s) 570
KspI CCGCGG 2 cut(s) 513, 595
Kzo9I GATC 2 cut(s) 570, 605
Lsp1109I GCAGC 3 cut(s) 319, 369, 397
LweI GCATC 1 cut(s) 119
MaeI CTAG 2 cut(s) 543, 609
MaeII ACGT 3 cut(s) 404, 434, 467
MaeIII GTNAC 1 cut(s) 547
MalI GATC 2 cut(s) 572, 607
MboI GATC 2 cut(s) 570, 605
MboII GAAGA 2 cut(s) 11, 538
MflI RGATCY 1 cut(s) 605
MmeI TCCRAC 1 cut(s) 201
MroNI GCCGGC 1 cut(s) 185
MseI TTAA 1 cut(s) 531
MspA1I CMGCKG 2 cut(s) 512, 594
MspI CCGG 2 cut(s) 186, 394
MspR9I CCNGG 4 cut(s) 218, 293, 395, 473
Mva1269I GAATGC 1 cut(s) 541
MvaI CCWGG 3 cut(s) 218, 293, 473
MvnI CGCG 6 cut(s) 207, 246, 255, 512, 560, 594
NaeI GCCGGC 1 cut(s) 187
NciI CCSGG 1 cut(s) 395
NdeII GATC 2 cut(s) 570, 605
NgoMIV GCCGGC 1 cut(s) 185
NlaIII CATG 1 cut(s) 135
NlaIV GGNNCC 1 cut(s) 190
NmeAIII GCCGAG 2 cut(s) 293, 354
PcsI WCGNNNNNNNCGW 1 cut(s) 363
PctI GAATGC 1 cut(s) 541
PdiI GCCGGC 1 cut(s) 187
PfeI GAWTC 1 cut(s) 481
PfoI TCCNGGA 1 cut(s) 393
PmaCI CACGTG 1 cut(s) 468
PmlI CACGTG 1 cut(s) 468
Ppu21I YACGTR 1 cut(s) 468
Psp6I CCWGG 3 cut(s) 216, 291, 471
PspCI CACGTG 1 cut(s) 468
PspFI CCCAGC 2 cut(s) 106, 273
PspGI CCWGG 3 cut(s) 216, 291, 471
PspN4I GGNNCC 1 cut(s) 190
PspPI GGNCC 3 cut(s) 188, 334, 600
PstI CTGCAG 1 cut(s) 387
PsuI RGATCY 1 cut(s) 605
RsaI GTAC 1 cut(s) 566
RsaNI GTAC 1 cut(s) 565
SacII CCGCGG 2 cut(s) 513, 595
SaqAI TTAA 1 cut(s) 531
Sau3AI GATC 2 cut(s) 570, 605
Sau96I GGNCC 3 cut(s) 188, 334, 600
ScrFI CCNGG 4 cut(s) 218, 293, 395, 473
SfaNI GCATC 1 cut(s) 119
SfcI CTRYAG 1 cut(s) 383
Sfr303I CCGCGG 2 cut(s) 513, 595
SgrBI CCGCGG 2 cut(s) 513, 595
SinI GGWCC 1 cut(s) 334
SspMI CTAG 2 cut(s) 543, 609
StyD4I CCNGG 4 cut(s) 216, 291, 393, 471
StyI CCWWGG 1 cut(s) 504
TaiI ACGT 3 cut(s) 407, 437, 470
TfiI GAWTC 1 cut(s) 481
Tru1I TTAA 1 cut(s) 531
Tru9I TTAA 1 cut(s) 531
TseI GCWGC 3 cut(s) 307, 382, 385
TspDTI ATGAA 1 cut(s) 117
TspGWI ACGGA 3 cut(s) 113, 214, 470
VpaK11BI GGWCC 1 cut(s) 334
XagI CCTNNNNNAGG 2 cut(s) 215, 395
XbaI TCTAGA 1 cut(s) 608
XspI CTAG 2 cut(s) 543, 609
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.