AT5G22340

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0005470
Physical Location & Seq
Forward (+)
7394443 .. 7397320
2878 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G22340.2

Sequence Viewer

Length: 972 bp
ATGGAGAGTCTCTCGATTCCCGCCGCTTCTTGGTTTTCCGGCGGAACCATTCCTCCGACGAAGGTGAGAGTTTCTCCGCCGCATCATCTCCGGCGACCTCCCAACAGGAATTCCCATCGCTTGGCCGCCATTAATGCCGCTTCTCGCTCTGTGAGTGAGCTAGTGGAGGAGGATGTGTTGCAAATGTTCATGAAAGACAGAGAAGAAAATGGAGATTTCATATCTAAAGTTTCTGATAGGCTCTGGTTAAAGGAGATTCTCGAATCTATTGATGTTAATTCCAATGGAGCTTCTTCTTCAGCTGTTGAACTAGATGATACTCGGGAAAGCTTGATAGATGGCGCTGATGATGATGAAAGTGGTTTCTTGAAACTTAAACCGACACAAGAATGGATTGGTTGGGAGAGTGATTCTGCCCCCATGAATAAGAAAGCTCTAGCTAAGGCACTACGAGATGACAGCGAGAGAAGGAAGAAGTTTAACTTTCTGAAATATGAAGCTCTGAAGCGTGAACTCATGTACCTGTCGATTGTAATTGGAACTGGATGCAGCGGATACTGTTTACTAGCTTTATCAGTTCAGGCTGCAGTCAGTTATGCAGTTGGGGTTCTATTCAGTTGCTTGTACCTTCAGCTTTTGTATGGATACGCTGATGGTCTATCACGTGAAGCTGTTCCTGATATCTTCCTGAAAAAAAAGTCTAAAAAAATCGGGATCAGAAGTGAAGACCTTGAGGACTTTGTAGTGAGGACAATCAGGGGCAGTGGCATGGCTCTCTCTTCTCCACGCTTGGTGATACCAGCTGCAATTTATGGTTTGTGGATACTCTCTCACAAGTATTTCCAGAACGACTTGTTCGACTTTCAGATAGTTCCAGCAATGGTTGGTTTGTTTGTATACAAAGCTGCAACTCTCGTTCAAGTGTACAGAGACAACCAAGATCTTCAGTTCATCTTCCCAGATGACTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

323

Amino Acids

36.27

Weight (kDa)

5.72

Isoelectric Point (pI)

41.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CGL160_C PF24763 164 - 224 9.2e-06 CGL160, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 121
AccI GTMKAC 1 cut(s) 897
AciI CCGC 8 cut(s) 21, 24, 42, 77, 80, 126, 138, 552
AclWI GGATC 1 cut(s) 722
AcoI YGGCCR 1 cut(s) 123
AcsI RAATTY 1 cut(s) 109
AcuI CTGAAG 4 cut(s) 282, 524, 614, 929
AcvI CACGTG 1 cut(s) 665
AfaI GTAC 3 cut(s) 521, 626, 926
AfiI CCNNNNNNNGG 2 cut(s) 30, 121
AgsI TTSAA 3 cut(s) 308, 370, 920
AloI GAACNNNNNNTCC 2 cut(s) 37, 69
Alw26I GTCTC 2 cut(s) 14, 924
AlwI GGATC 1 cut(s) 722
Ama87I CYCGRG 1 cut(s) 321
AoxI GGCC 1 cut(s) 123
ApeKI GCWGC 4 cut(s) 549, 584, 803, 905
ApoI RAATTY 1 cut(s) 109
AseI ATTAAT 1 cut(s) 132
Asp700I GAANNNNTTC 1 cut(s) 672
AspLEI GCGC 1 cut(s) 344
AsuHPI GGTGA 2 cut(s) 76, 805
AvaI CYCGRG 1 cut(s) 321
BaeI ACNNNNGTAYC 2 cut(s) 503, 536
BbrPI CACGTG 1 cut(s) 665
BbsI GAAGAC 1 cut(s) 732
BbvI GCAGC 4 cut(s) 561, 571, 790, 892
BccI CCATC 3 cut(s) 123, 332, 647
BciVI GTATCC 3 cut(s) 548, 638, 816
BcoDI GTCTC 2 cut(s) 14, 924
BfaI CTAG 4 cut(s) 161, 311, 437, 566
BfmI CTRYAG 1 cut(s) 585
BfoI RGCGCY 1 cut(s) 345
BfuI GTATCC 3 cut(s) 548, 638, 816
BglII AGATCT 1 cut(s) 940
BisI GCNGC 8 cut(s) 24, 80, 126, 138, 550, 585, 804, 906
BlsI GCNGC 8 cut(s) 25, 81, 127, 139, 551, 586, 805, 907
BmeT110I CYCGRG 1 cut(s) 321
BmiI GGNNCC 1 cut(s) 46
BmsI GCATC 2 cut(s) 91, 536
BpiI GAAGAC 1 cut(s) 732
BplI GAGNNNNNCTC 3 cut(s) 28, 58, 90
Bpu10I CCTNAGC 1 cut(s) 441
BpuEI CTTGAG 1 cut(s) 752
BsaAI YACGTR 1 cut(s) 665
BsaXI ACNNNNNCTCC 4 cut(s) 37, 67, 161, 191
Bsc4I CCNNNNNNNGG 2 cut(s) 30, 121
Bse1I ACTGG 1 cut(s) 547
Bse3DI GCAATG 1 cut(s) 885
BseGI GGATG 2 cut(s) 178, 551
BseLI CCNNNNNNNGG 2 cut(s) 30, 121
BseMI GCAATG 1 cut(s) 885
BseNI ACTGG 1 cut(s) 547
BseRI GAGGAG 1 cut(s) 182
BseXI GCAGC 4 cut(s) 561, 571, 790, 892
BshFI GGCC 1 cut(s) 125
BsiHKCI CYCGRG 1 cut(s) 321
BsiSI CCGG 2 cut(s) 39, 91
BslI CCNNNNNNNGG 2 cut(s) 30, 121
BsmAI GTCTC 2 cut(s) 14, 924
BsnI GGCC 1 cut(s) 125
BsoBI CYCGRG 1 cut(s) 321
Bsp1407I TGTACA 1 cut(s) 924
Bsp143I GATC 2 cut(s) 714, 940
BspACI CCGC 8 cut(s) 21, 24, 42, 77, 80, 126, 138, 552
BspANI GGCC 1 cut(s) 125
BspHI TCATGA 1 cut(s) 189
BspLI GGNNCC 1 cut(s) 46
BspMAI CTGCAG 1 cut(s) 589
BspPI GGATC 1 cut(s) 722
BsrDI GCAATG 1 cut(s) 885
BsrGI TGTACA 1 cut(s) 924
BsrI ACTGG 1 cut(s) 547
BssMI GATC 2 cut(s) 714, 940
BssNAI GTATAC 1 cut(s) 898
Bst1107I GTATAC 1 cut(s) 898
Bst4CI ACNGT 1 cut(s) 560
Bst6I CTCTTC 1 cut(s) 784
BstAUI TGTACA 1 cut(s) 924
BstBAI YACGTR 1 cut(s) 665
BstDEI CTNAG 1 cut(s) 441
BstF5I GGATG 2 cut(s) 178, 551
BstH2I RGCGCY 1 cut(s) 345
BstHHI GCGC 1 cut(s) 344
BstKTI GATC 2 cut(s) 717, 943
BstMAI GTCTC 2 cut(s) 14, 924
BstMBI GATC 2 cut(s) 714, 940
BstMWI GCNNNNNNNGC 1 cut(s) 134
BstSFI CTRYAG 1 cut(s) 585
BstV1I GCAGC 4 cut(s) 561, 571, 790, 892
BstV2I GAAGAC 1 cut(s) 732
BstX2I RGATCY 1 cut(s) 940
BstYI RGATCY 1 cut(s) 940
BstZ17I GTATAC 1 cut(s) 898
BsuI GTATCC 3 cut(s) 548, 638, 816
BsuRI GGCC 1 cut(s) 125
BtgZI GCGATG 1 cut(s) 101
BtsCI GGATG 2 cut(s) 178, 551
BtsI GCAGTG 1 cut(s) 769
BtsIMutI CAGTG 1 cut(s) 769
CciI TCATGA 1 cut(s) 189
CfoI GCGC 1 cut(s) 344
Csp6I GTAC 3 cut(s) 520, 625, 925
CviAII CATG 4 cut(s) 190, 421, 517, 769
CviQI GTAC 3 cut(s) 520, 625, 925
DdeI CTNAG 1 cut(s) 441
DpnI GATC 2 cut(s) 716, 942
DpnII GATC 2 cut(s) 714, 940
EaeI YGGCCR 1 cut(s) 123
Eam1104I CTCTTC 1 cut(s) 784
EarI CTCTTC 1 cut(s) 784
EciI GGCGGA 2 cut(s) 57, 66
Eco32I GATATC 1 cut(s) 682
Eco57I CTGAAG 4 cut(s) 282, 524, 614, 929
Eco72I CACGTG 1 cut(s) 665
Eco88I CYCGRG 1 cut(s) 321
EcoRI GAATTC 1 cut(s) 109
EcoRV GATATC 1 cut(s) 682
FaeI CATG 4 cut(s) 193, 424, 520, 772
FalI AAGNNNNNCTT 2 cut(s) 467, 499
FatI CATG 4 cut(s) 189, 420, 516, 768
FauI CCCGC 1 cut(s) 28
FblI GTMKAC 1 cut(s) 897
Fnu4HI GCNGC 8 cut(s) 24, 80, 126, 138, 550, 585, 804, 906
FokI GGATG 2 cut(s) 185, 558
Fsp4HI GCNGC 8 cut(s) 24, 80, 126, 138, 550, 585, 804, 906
FspBI CTAG 4 cut(s) 161, 311, 437, 566
GlaI GCGC 1 cut(s) 343
GluI GCNGC 8 cut(s) 24, 80, 126, 138, 550, 585, 804, 906
HaeII RGCGCY 1 cut(s) 345
HaeIII GGCC 1 cut(s) 125
HapII CCGG 2 cut(s) 39, 91
HhaI GCGC 1 cut(s) 344
Hin1II CATG 4 cut(s) 193, 424, 520, 772
Hin6I GCGC 1 cut(s) 342
HinP1I GCGC 1 cut(s) 342
HindIII AAGCTT 1 cut(s) 328
HinfI GANTC 5 cut(s) 7, 16, 256, 263, 410
HpaII CCGG 2 cut(s) 39, 91
HphI GGTGA 2 cut(s) 76, 805
Hpy166II GTNNAC 4 cut(s) 512, 563, 898, 925
Hpy188I TCNGA 6 cut(s) 57, 235, 489, 504, 719, 867
Hpy188III TCNNGA 9 cut(s) 13, 190, 260, 323, 367, 677, 688, 712, 844
Hpy8I GTNNAC 4 cut(s) 512, 563, 898, 925
Hpy99I CGWCG 1 cut(s) 61
HpyAV CCTTC 3 cut(s) 55, 462, 638
HpyCH4III ACNGT 1 cut(s) 560
HpyCH4IV ACGT 1 cut(s) 664
HpyCH4V TGCA 6 cut(s) 181, 549, 587, 599, 806, 908
HpyF10VI GCNNNNNNNGC 1 cut(s) 134
HpyF3I CTNAG 1 cut(s) 441
HpySE526I ACGT 1 cut(s) 664
Hsp92II CATG 4 cut(s) 193, 424, 520, 772
HspAI GCGC 1 cut(s) 342
Kzo9I GATC 2 cut(s) 714, 940
LmnI GCTCC 1 cut(s) 287
Lsp1109I GCAGC 4 cut(s) 561, 571, 790, 892
LweI GCATC 2 cut(s) 91, 536
MaeI CTAG 4 cut(s) 161, 311, 437, 566
MaeII ACGT 1 cut(s) 664
MalI GATC 2 cut(s) 716, 942
MboI GATC 2 cut(s) 714, 940
MboII GAAGA 9 cut(s) 215, 285, 288, 484, 676, 737, 771, 935, 946
MflI RGATCY 1 cut(s) 940
MluCI AATT 4 cut(s) 109, 277, 534, 807
MlyI GAGTC 1 cut(s) 16
MmeI TCCRAC 1 cut(s) 80
MnlI CCTC 6 cut(s) 63, 108, 160, 163, 727, 741
MroXI GAANNNNTTC 1 cut(s) 672
MseI TTAA 5 cut(s) 132, 248, 276, 375, 480
MslI CAYNNNNRTG 1 cut(s) 388
MspA1I CMGCKG 3 cut(s) 302, 552, 803
MspI CCGG 2 cut(s) 39, 91
MwoI GCNNNNNNNGC 1 cut(s) 134
NdeII GATC 2 cut(s) 714, 940
NlaIII CATG 4 cut(s) 193, 424, 520, 772
NlaIV GGNNCC 1 cut(s) 46
PagI TCATGA 1 cut(s) 189
PcsI WCGNNNNNNNCGW 1 cut(s) 855
PdmI GAANNNNTTC 1 cut(s) 672
PfeI GAWTC 4 cut(s) 16, 256, 263, 410
PflMI CCANNNNNTGG 1 cut(s) 121
PkrI GCNGC 8 cut(s) 25, 81, 127, 139, 551, 586, 805, 907
PleI GAGTC 1 cut(s) 15
PmaCI CACGTG 1 cut(s) 665
PmlI CACGTG 1 cut(s) 665
PpsI GAGTC 1 cut(s) 15
Ppu21I YACGTR 1 cut(s) 665
PshBI ATTAAT 1 cut(s) 132
PspCI CACGTG 1 cut(s) 665
PspN4I GGNNCC 1 cut(s) 46
PstI CTGCAG 1 cut(s) 589
PsuI RGATCY 1 cut(s) 940
PvuII CAGCTG 2 cut(s) 302, 803
RsaI GTAC 3 cut(s) 521, 626, 926
RsaNI GTAC 3 cut(s) 520, 625, 925
RseI CAYNNNNRTG 1 cut(s) 388
SaqAI TTAA 5 cut(s) 132, 248, 276, 375, 480
SatI GCNGC 8 cut(s) 24, 80, 126, 138, 550, 585, 804, 906
Sau3AI GATC 2 cut(s) 714, 940
SchI GAGTC 1 cut(s) 16
SfaNI GCATC 2 cut(s) 91, 536
SfcI CTRYAG 1 cut(s) 585
SmiMI CAYNNNNRTG 1 cut(s) 388
SmlI CTYRAG 1 cut(s) 731
SmoI CTYRAG 1 cut(s) 731
Sse9I AATT 4 cut(s) 109, 277, 534, 807
SsiI CCGC 8 cut(s) 21, 24, 42, 77, 80, 126, 138, 552
SspMI CTAG 4 cut(s) 161, 311, 437, 566
TaaI ACNGT 1 cut(s) 560
TaiI ACGT 1 cut(s) 667
TaqI TCGA 4 cut(s) 14, 261, 527, 858
TasI AATT 4 cut(s) 109, 277, 534, 807
TatI WGTACW 1 cut(s) 924
TauI GCSGC 4 cut(s) 26, 82, 128, 140
TfiI GAWTC 4 cut(s) 16, 256, 263, 410
Tru1I TTAA 5 cut(s) 132, 248, 276, 375, 480
Tru9I TTAA 5 cut(s) 132, 248, 276, 375, 480
TscAI CASTG 1 cut(s) 769
TseI GCWGC 4 cut(s) 549, 584, 803, 905
TspDTI ATGAA 7 cut(s) 178, 206, 208, 369, 437, 510, 940
TspRI CASTG 1 cut(s) 769
Van91I CCANNNNNTGG 1 cut(s) 121
VspI ATTAAT 1 cut(s) 132
XapI RAATTY 1 cut(s) 109
XmiI GTMKAC 1 cut(s) 897
XmnI GAANNNNTTC 1 cut(s) 672
XspI CTAG 4 cut(s) 161, 311, 437, 566
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.