FvH4_2g14080

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0005470
Physical Location & Seq
Reverse (-)
12378830 .. 12384106
5277 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g14080.t5

Sequence Viewer

Length: 912 bp
ATGGAGACCCTTTTCAGCTCCTCATTCCGATTAACTACTCCAATCCATCATCCTCGCTCACTTTCCAGCAGAAACACATCTGTCTCGGCCCTCAGAACAACCCCATCTCCTGTTGTAGAGGAGGACTTGCTGCAGACATTCTTTAAGGATAGACAGTTGAATGGAGACTTTGTTTCAAGACTTTCCGACGTGTTTTGGCGGAGGGAATTTACCAATTTTGTTGATGGTGATCAAGTCGGTCAAAGTGTTGATATTACTACTCAACAACAAGCCGCACAAGAGCAGGTTGTGGATGATGCTGGTGGGTTTTTGAAACTGTCAGCAACCAATGAATGGGTGTTAGGTGATAGATCTGCACCAATTAATAAAAAGGCCATTGCCAAGGCCATGCAGAATGACAGTGAAAGAAGAAAGAAACTCAACCTTCTTCAATATGAAGCTATACAGAGGGAACTAATGCTTTTGTCTATTGGTATTGGAACTGCTTGTACTGGGTATTGTCTTGTAACACTGTCATTCCAGGGTGCTATCAGTTATGCGACAGGAGTTCTATTCAGTTGCTTGTACCTTAAACTCTTGTACCAACATGTGGACAATATATCCCGAGAAGCAGTCCCTCAGATTTTCAGGCAGAAGAAATCGAAGAAAATTGGAATAAGAAGTCAGGATCTGGAGGATTCTCTAGAGAAATTGATTAAGGGCTGTGGTATGGCTCTTTCATCTCCAAGGCTTGTGTTACCAGCAGCAATATATGGATTGTGGATCCTGTCTCATCAATATGTTTCAAGTGATCTCTTTGATTTTCAGCTTGTGCCAGCCATGGTTGGGTTGTTTGTTTATAAAGCTGCTGCTCTTGTTCAAGTCTATAGAGATAATGAAGACCTGCAATTGATCTTTCCTGATGACCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

304

Amino Acids

34.03

Weight (kDa)

6.44

Isoelectric Point (pI)

43.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CGL160_C PF24763 144 - 205 1.1e-07 CGL160, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 840
Acc36I ACCTGC 2 cut(s) 274, 891
AccB7I CCANNNNNTGG 2 cut(s) 333, 589
AciI CCGC 2 cut(s) 199, 273
AclWI GGATC 3 cut(s) 675, 757, 770
AcsI RAATTY 1 cut(s) 206
AfaI GTAC 3 cut(s) 490, 566, 581
AfiI CCNNNNNNNGG 3 cut(s) 333, 589, 825
AflIII ACRYGT 2 cut(s) 189, 586
AgsI TTSAA 6 cut(s) 160, 177, 313, 431, 786, 860
AjiI CACGTC 1 cut(s) 190
AjnI CCWGG 1 cut(s) 519
AluBI AGCT 4 cut(s) 18, 440, 808, 845
AluI AGCT 4 cut(s) 18, 440, 808, 845
Alw26I GTCTC 3 cut(s) 88, 159, 774
AlwI GGATC 3 cut(s) 675, 757, 770
AlwNI CAGNNNCTG 1 cut(s) 670
Ama87I CYCGRG 1 cut(s) 603
AoxI GGCC 3 cut(s) 87, 372, 384
ApeKI GCWGC 4 cut(s) 130, 743, 845, 848
ApoI RAATTY 1 cut(s) 206
AseI ATTAAT 1 cut(s) 363
AspS9I GGNCC 1 cut(s) 88
AsuHPI GGTGA 2 cut(s) 239, 356
AvaI CYCGRG 1 cut(s) 603
BaeI ACNNNNGTAYC 2 cut(s) 563, 596
BamHI GGATCC 1 cut(s) 762
BbsI GAAGAC 1 cut(s) 885
BbvI GCAGC 4 cut(s) 117, 755, 832, 835
BccI CCATC 3 cut(s) 54, 112, 218
BciT130I CCWGG 1 cut(s) 521
BclI TGATCA 1 cut(s) 229
BcoDI GTCTC 3 cut(s) 88, 159, 774
BfaI CTAG 1 cut(s) 683
BfmI CTRYAG 2 cut(s) 131, 865
BfuAI ACCTGC 2 cut(s) 274, 891
BglII AGATCT 1 cut(s) 350
BisI GCNGC 5 cut(s) 131, 273, 744, 846, 849
BlsI GCNGC 5 cut(s) 132, 274, 745, 847, 850
Bme1390I CCNGG 1 cut(s) 521
BmeT110I CYCGRG 1 cut(s) 603
BmgBI CACGTC 1 cut(s) 190
BmgT120I GGNCC 1 cut(s) 88
BmiI GGNNCC 1 cut(s) 764
BmrFI CCNGG 1 cut(s) 521
BmrI ACTGGG 1 cut(s) 501
BmsI GCATC 1 cut(s) 286
BmuI ACTGGG 1 cut(s) 501
BpiI GAAGAC 1 cut(s) 885
BpmI CTGGAG 1 cut(s) 692
BsaBI GATNNNNATC 1 cut(s) 228
BsaJI CCNNGG 4 cut(s) 381, 520, 725, 819
BsaXI ACNNNNNCTCC 4 cut(s) 91, 121, 156, 186
Bsc4I CCNNNNNNNGG 3 cut(s) 333, 589, 825
Bse1I ACTGG 1 cut(s) 496
Bse3DI GCAATG 1 cut(s) 375
Bse8I GATNNNNATC 1 cut(s) 228
BseBI CCWGG 1 cut(s) 521
BseDI CCNNGG 4 cut(s) 381, 520, 725, 819
BseGI GGATG 2 cut(s) 49, 298
BseJI GATNNNNATC 1 cut(s) 228
BseLI CCNNNNNNNGG 3 cut(s) 333, 589, 825
BseMI GCAATG 1 cut(s) 375
BseMII CTCAG 2 cut(s) 106, 632
BseNI ACTGG 1 cut(s) 496
BseRI GAGGAG 2 cut(s) 10, 134
BseXI GCAGC 4 cut(s) 117, 755, 832, 835
BsgI GTGCAG 1 cut(s) 339
BshFI GGCC 3 cut(s) 89, 374, 386
BsiHKCI CYCGRG 1 cut(s) 603
BslFI GGGAC 1 cut(s) 599
BslI CCNNNNNNNGG 3 cut(s) 333, 589, 825
BsmAI GTCTC 3 cut(s) 88, 159, 774
BsmFI GGGAC 1 cut(s) 599
BsnI GGCC 3 cut(s) 89, 374, 386
BsoBI CYCGRG 1 cut(s) 603
Bsp143I GATC 6 cut(s) 229, 350, 667, 762, 790, 891
Bsp19I CCATGG 1 cut(s) 819
BspACI CCGC 2 cut(s) 199, 273
BspANI GGCC 3 cut(s) 89, 374, 386
BspCNI CTCAG 2 cut(s) 105, 631
BspLI GGNNCC 1 cut(s) 764
BspMAI CTGCAG 1 cut(s) 135
BspMI ACCTGC 2 cut(s) 274, 891
BspPI GGATC 3 cut(s) 675, 757, 770
BsrDI GCAATG 1 cut(s) 375
BsrI ACTGG 1 cut(s) 496
BssECI CCNNGG 4 cut(s) 381, 520, 725, 819
BssMI GATC 6 cut(s) 229, 350, 667, 762, 790, 891
BssT1I CCWWGG 3 cut(s) 381, 725, 819
Bst2UI CCWGG 1 cut(s) 521
Bst4CI ACNGT 4 cut(s) 156, 318, 401, 513
BstC8I GCNNGC 1 cut(s) 816
BstDEI CTNAG 2 cut(s) 92, 618
BstDSI CCRYGG 1 cut(s) 819
BstF5I GGATG 2 cut(s) 49, 298
BstKTI GATC 6 cut(s) 232, 353, 670, 765, 793, 894
BstMAI GTCTC 3 cut(s) 88, 159, 774
BstMBI GATC 6 cut(s) 229, 350, 667, 762, 790, 891
BstNI CCWGG 1 cut(s) 521
BstNSI RCATGY 1 cut(s) 590
BstSCI CCNGG 1 cut(s) 519
BstSFI CTRYAG 2 cut(s) 131, 865
BstV1I GCAGC 4 cut(s) 117, 755, 832, 835
BstV2I GAAGAC 1 cut(s) 885
BstX2I RGATCY 3 cut(s) 350, 667, 762
BstYI RGATCY 3 cut(s) 350, 667, 762
BsuRI GGCC 3 cut(s) 89, 374, 386
BtgI CCRYGG 1 cut(s) 819
BtrI CACGTC 1 cut(s) 190
BtsCI GGATG 2 cut(s) 49, 298
BtsIMutI CAGTG 2 cut(s) 406, 509
BveI ACCTGC 2 cut(s) 274, 891
Cac8I GCNNGC 1 cut(s) 816
CaiI CAGNNNCTG 1 cut(s) 670
Cfr13I GGNCC 1 cut(s) 88
Csp6I GTAC 3 cut(s) 489, 565, 580
CviAII CATG 3 cut(s) 388, 587, 820
CviQI GTAC 3 cut(s) 489, 565, 580
DdeI CTNAG 2 cut(s) 92, 618
DpnI GATC 6 cut(s) 231, 352, 669, 764, 792, 893
DpnII GATC 6 cut(s) 229, 350, 667, 762, 790, 891
EciI GGCGGA 1 cut(s) 214
Eco130I CCWWGG 3 cut(s) 381, 725, 819
Eco88I CYCGRG 1 cut(s) 603
EcoRII CCWGG 1 cut(s) 519
EcoT14I CCWWGG 3 cut(s) 381, 725, 819
ErhI CCWWGG 3 cut(s) 381, 725, 819
FaeI CATG 3 cut(s) 391, 590, 823
FaqI GGGAC 1 cut(s) 599
FatI CATG 3 cut(s) 387, 586, 819
FbaI TGATCA 1 cut(s) 229
Fnu4HI GCNGC 5 cut(s) 131, 273, 744, 846, 849
FokI GGATG 2 cut(s) 36, 305
Fsp4HI GCNGC 5 cut(s) 131, 273, 744, 846, 849
FspBI CTAG 1 cut(s) 683
GluI GCNGC 5 cut(s) 131, 273, 744, 846, 849
GsuI CTGGAG 1 cut(s) 692
HaeIII GGCC 3 cut(s) 89, 374, 386
Hin1II CATG 3 cut(s) 391, 590, 823
HinfI GANTC 1 cut(s) 677
HphI GGTGA 2 cut(s) 239, 356
Hpy166II GTNNAC 1 cut(s) 592
Hpy188I TCNGA 4 cut(s) 29, 95, 187, 621
Hpy188III TCNNGA 6 cut(s) 177, 603, 665, 671, 683, 899
Hpy8I GTNNAC 1 cut(s) 592
Hpy99I CGWCG 1 cut(s) 191
HpyAV CCTTC 1 cut(s) 434
HpyCH4III ACNGT 4 cut(s) 156, 318, 401, 513
HpyCH4IV ACGT 1 cut(s) 189
HpyCH4V TGCA 4 cut(s) 133, 356, 391, 886
HpyF3I CTNAG 2 cut(s) 92, 618
HpySE526I ACGT 1 cut(s) 189
Hsp92II CATG 3 cut(s) 391, 590, 823
Ksp22I TGATCA 1 cut(s) 229
Kzo9I GATC 6 cut(s) 229, 350, 667, 762, 790, 891
LmnI GCTCC 1 cut(s) 23
Lsp1109I GCAGC 4 cut(s) 117, 755, 832, 835
LweI GCATC 1 cut(s) 286
MaeI CTAG 1 cut(s) 683
MaeII ACGT 1 cut(s) 189
MaeIII GTNAC 2 cut(s) 505, 735
MalI GATC 6 cut(s) 231, 352, 669, 764, 792, 893
MboI GATC 6 cut(s) 229, 350, 667, 762, 790, 891
MboII GAAGA 5 cut(s) 419, 420, 646, 655, 890
MfeI CAATTG 1 cut(s) 887
MflI RGATCY 3 cut(s) 350, 667, 762
MluCI AATT 6 cut(s) 206, 214, 360, 648, 689, 887
MmeI TCCRAC 1 cut(s) 210
MnlI CCTC 9 cut(s) 31, 63, 101, 112, 115, 195, 441, 627, 667
MseI TTAA 5 cut(s) 32, 144, 363, 570, 696
MslI CAYNNNNRTG 1 cut(s) 777
MspR9I CCNGG 1 cut(s) 521
MunI CAATTG 1 cut(s) 887
MvaI CCWGG 1 cut(s) 521
NcoI CCATGG 1 cut(s) 819
NdeII GATC 6 cut(s) 229, 350, 667, 762, 790, 891
NlaIII CATG 3 cut(s) 391, 590, 823
NlaIV GGNNCC 1 cut(s) 764
NmeAIII GCCGAG 1 cut(s) 65
NspI RCATGY 1 cut(s) 590
PciI ACATGT 1 cut(s) 586
PfeI GAWTC 1 cut(s) 677
PflMI CCANNNNNTGG 2 cut(s) 333, 589
PkrI GCNGC 5 cut(s) 132, 274, 745, 847, 850
PscI ACATGT 1 cut(s) 586
PshBI ATTAAT 1 cut(s) 363
PsiI TTATAA 1 cut(s) 840
Psp6I CCWGG 1 cut(s) 519
PspGI CCWGG 1 cut(s) 519
PspN4I GGNNCC 1 cut(s) 764
PspPI GGNCC 1 cut(s) 88
PsrI GAACNNNNNNTAC 2 cut(s) 472, 504
PstI CTGCAG 1 cut(s) 135
PstNI CAGNNNCTG 1 cut(s) 670
PsuI RGATCY 3 cut(s) 350, 667, 762
RsaI GTAC 3 cut(s) 490, 566, 581
RsaNI GTAC 3 cut(s) 489, 565, 580
RseI CAYNNNNRTG 1 cut(s) 777
SaqAI TTAA 5 cut(s) 32, 144, 363, 570, 696
SatI GCNGC 5 cut(s) 131, 273, 744, 846, 849
Sau3AI GATC 6 cut(s) 229, 350, 667, 762, 790, 891
Sau96I GGNCC 1 cut(s) 88
ScrFI CCNGG 1 cut(s) 521
SfaNI GCATC 1 cut(s) 286
SfcI CTRYAG 2 cut(s) 131, 865
SmiMI CAYNNNNRTG 1 cut(s) 777
Sse9I AATT 6 cut(s) 206, 214, 360, 648, 689, 887
SsiI CCGC 2 cut(s) 199, 273
SspMI CTAG 1 cut(s) 683
StyD4I CCNGG 1 cut(s) 519
StyI CCWWGG 3 cut(s) 381, 725, 819
TaaI ACNGT 4 cut(s) 156, 318, 401, 513
TaiI ACGT 1 cut(s) 192
TaqI TCGA 1 cut(s) 641
TaqII GACCGA 1 cut(s) 227
TasI AATT 6 cut(s) 206, 214, 360, 648, 689, 887
TatI WGTACW 1 cut(s) 488
TauI GCSGC 1 cut(s) 275
TfiI GAWTC 1 cut(s) 677
Tru1I TTAA 5 cut(s) 32, 144, 363, 570, 696
Tru9I TTAA 5 cut(s) 32, 144, 363, 570, 696
TscAI CASTG 2 cut(s) 406, 516
TseI GCWGC 4 cut(s) 130, 743, 845, 848
TspDTI ATGAA 4 cut(s) 345, 450, 708, 891
TspRI CASTG 2 cut(s) 406, 516
Van91I CCANNNNNTGG 2 cut(s) 333, 589
VspI ATTAAT 1 cut(s) 363
XapI RAATTY 1 cut(s) 206
XbaI TCTAGA 1 cut(s) 682
XceI RCATGY 1 cut(s) 590
XspI CTAG 1 cut(s) 683
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.