pycom03g10380

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0005470
Physical Location & Seq
Reverse (-)
9538785 .. 9543375
4591 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g10380.2

Sequence Viewer

Length: 930 bp
ATGGAGACCCTTCTCAGGGTTGAGTCCTTCTCCTTCCCAAGAACCCTTCCAATCCCTAATCGTTCACTTCCCAATCGAATCCGAACTGTCTCTGCCCTCAGAACAACCCCACCTCCTGTGAGTGAAGTTGCAGAGGAGGACGTTCTGCAGACATTTTTCAAGGAAAGGCAATTGAATGGAGACTTGGTGTCAAAAGTTTCTGATGTGTTTTGGAAGCGGGAGTTTGCCAATTCTGTGGATGCTAATGATGGTGCCGAATTTGGTGATACTCCTCAGCTAGCAGAGCAGGTCACTGAAAATGATGCTGGTGGATTTTTGAAATTGTCAACAACCAACGAGTGGGTTCTAGGTGACATATCTGCACCAGTAAATAGAAAAGCCAGAGCTAAGGCCTTGCAGAATGACAGTGACAGAAGGAGGAAACTCAACCTTCTTCAGTATGAAGCTTTACAGAGGGAATTAATGCTGTTATCCATTGGTATTGGAACTGCATGTACTGGGTATTGCTTAATAGCATTGTCATCCCAGGCCGCTATCAGTTATGCCACGGGAGTTCTTTTCAGTTGCTTGTACCTTAAACTCTTGTACCAGCAAGCAGACAATATATCCAGAGACGCAGTCCCTCAAATTTTTAGGCAGAGGAAGACTAAGAAAATTGGAATAAGAAGCGAGGATTTGGAGGATTTTCTAGAGAAAACGATAAAGGGGTGTGGTATTGCGCTTTCATCTCCAAGGCTTGTAATACCAGCGGCAATATATGGATTGTGGATTCTGTCTCATCAAAAGTTTGCCGGTGATCTGTTTGATTTCCAGCTTGTGCCAGCTATGCTCGGGTTGTTTGTGTATAAGGCCGCTGCGCTATATCAAGTTTATAGAGATAACGAAGACTTGCAATTTATCTTTCCGGATAATGAAGAAGGCTCAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

310

Amino Acids

34.64

Weight (kDa)

5.71

Isoelectric Point (pI)

44.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 277
AccB1I GGYRCC 1 cut(s) 251
AccB7I CCANNNNNTGG 1 cut(s) 339
AccIII TCCGGA 1 cut(s) 904
AciI CCGC 4 cut(s) 217, 531, 749, 852
AcsI RAATTY 2 cut(s) 257, 627
AcuI CTGAAG 1 cut(s) 419
AfaI GTAC 3 cut(s) 496, 572, 587
AfiI CCNNNNNNNGG 3 cut(s) 15, 16, 339
AgsI TTSAA 3 cut(s) 160, 175, 319
AhdI GACNNNNNGTC 1 cut(s) 187
AjnI CCWGG 1 cut(s) 525
AjuI GAANNNNNNNTTGG 2 cut(s) 167, 199
AluBI AGCT 5 cut(s) 277, 386, 446, 814, 824
AluI AGCT 5 cut(s) 277, 386, 446, 814, 824
Alw26I GTCTC 4 cut(s) 94, 174, 606, 780
Ama87I CYCGRG 1 cut(s) 830
Aor13HI TCCGGA 1 cut(s) 904
AoxI GGCC 3 cut(s) 390, 528, 849
ApeKI GCWGC 1 cut(s) 854
ApoI RAATTY 2 cut(s) 257, 627
AseI ATTAAT 1 cut(s) 461
AspLEI GCGC 2 cut(s) 721, 859
AsuHPI GGTGA 3 cut(s) 275, 362, 806
AsuNHI GCTAGC 1 cut(s) 277
AvaI CYCGRG 1 cut(s) 830
BaeI ACNNNNGTAYC 2 cut(s) 569, 602
BanI GGYRCC 1 cut(s) 251
BbsI GAAGAC 2 cut(s) 650, 891
BbvCI CCTCAGC 1 cut(s) 273
BbvI GCAGC 1 cut(s) 841
BccI CCATC 1 cut(s) 242
BciT130I CCWGG 1 cut(s) 527
BcoDI GTCTC 4 cut(s) 94, 174, 606, 780
BfaI CTAG 3 cut(s) 278, 347, 689
BfmI CTRYAG 1 cut(s) 146
BfuAI ACCTGC 1 cut(s) 277
BisI GCNGC 4 cut(s) 531, 750, 852, 855
BlsI GCNGC 4 cut(s) 532, 751, 853, 856
Bme1390I CCNGG 1 cut(s) 527
BmeRI GACNNNNNGTC 1 cut(s) 187
BmeT110I CYCGRG 1 cut(s) 830
BmiI GGNNCC 1 cut(s) 253
BmrFI CCNGG 1 cut(s) 527
BmrI ACTGGG 1 cut(s) 507
BmsI GCATC 2 cut(s) 229, 292
BmtI GCTAGC 1 cut(s) 281
BmuI ACTGGG 1 cut(s) 507
BpiI GAAGAC 2 cut(s) 650, 891
BplI GAGNNNNNCTC 2 cut(s) 14, 46
Bpu10I CCTNAGC 2 cut(s) 273, 387
BsaJI CCNNGG 3 cut(s) 525, 546, 731
BsaWI WCCGGW 1 cut(s) 904
BsaXI ACNNNNNCTCC 4 cut(s) 97, 127, 171, 201
Bsc4I CCNNNNNNNGG 3 cut(s) 15, 16, 339
Bse118I RCCGGY 1 cut(s) 791
Bse1I ACTGG 2 cut(s) 365, 502
BseAI TCCGGA 1 cut(s) 904
BseBI CCWGG 1 cut(s) 527
BseDI CCNNGG 3 cut(s) 525, 546, 731
BseGI GGATG 2 cut(s) 244, 521
BseLI CCNNNNNNNGG 3 cut(s) 15, 16, 339
BseMII CTCAG 3 cut(s) 28, 112, 287
BseNI ACTGG 2 cut(s) 365, 502
BseRI GAGGAG 2 cut(s) 149, 261
BseXI GCAGC 1 cut(s) 841
BsgI GTGCAG 1 cut(s) 345
BshFI GGCC 3 cut(s) 392, 530, 851
BshNI GGYRCC 1 cut(s) 251
BsiHKCI CYCGRG 1 cut(s) 830
BsiSI CCGG 2 cut(s) 792, 905
BslFI GGGAC 1 cut(s) 605
BslI CCNNNNNNNGG 3 cut(s) 15, 16, 339
BsmAI GTCTC 4 cut(s) 94, 174, 606, 780
BsmBI CGTCTC 1 cut(s) 606
BsmFI GGGAC 1 cut(s) 605
BsnI GGCC 3 cut(s) 392, 530, 851
BsoBI CYCGRG 1 cut(s) 830
Bsp13I TCCGGA 1 cut(s) 904
Bsp143I GATC 1 cut(s) 796
BspACI CCGC 4 cut(s) 217, 531, 749, 852
BspANI GGCC 3 cut(s) 392, 530, 851
BspCNI CTCAG 3 cut(s) 27, 111, 286
BspEI TCCGGA 1 cut(s) 904
BspLI GGNNCC 1 cut(s) 253
BspMAI CTGCAG 1 cut(s) 150
BspMI ACCTGC 1 cut(s) 277
BspOI GCTAGC 1 cut(s) 281
BspT107I GGYRCC 1 cut(s) 251
BsrFI RCCGGY 1 cut(s) 791
BsrI ACTGG 2 cut(s) 365, 502
BssAI RCCGGY 1 cut(s) 791
BssECI CCNNGG 3 cut(s) 525, 546, 731
BssMI GATC 1 cut(s) 796
BssT1I CCWWGG 1 cut(s) 731
Bst2UI CCWGG 1 cut(s) 527
Bst4CI ACNGT 2 cut(s) 88, 407
BstC8I GCNNGC 3 cut(s) 279, 594, 822
BstDEI CTNAG 5 cut(s) 14, 98, 273, 387, 648
BstDSI CCRYGG 1 cut(s) 546
BstF5I GGATG 2 cut(s) 244, 521
BstHHI GCGC 2 cut(s) 721, 859
BstKTI GATC 1 cut(s) 799
BstMAI GTCTC 4 cut(s) 94, 174, 606, 780
BstMBI GATC 1 cut(s) 796
BstMWI GCNNNNNNNGC 2 cut(s) 283, 826
BstNI CCWGG 1 cut(s) 527
BstNSI RCATGY 1 cut(s) 495
BstSCI CCNGG 1 cut(s) 525
BstSFI CTRYAG 1 cut(s) 146
BstV1I GCAGC 1 cut(s) 841
BstV2I GAAGAC 2 cut(s) 650, 891
BstXI CCANNNNNNTGG 1 cut(s) 235
BsuRI GGCC 3 cut(s) 392, 530, 851
BtgI CCRYGG 1 cut(s) 546
BtsCI GGATG 2 cut(s) 244, 521
BtsIMutI CAGTG 2 cut(s) 291, 412
BveI ACCTGC 1 cut(s) 277
Cac8I GCNNGC 3 cut(s) 279, 594, 822
CfoI GCGC 2 cut(s) 721, 859
Cfr10I RCCGGY 1 cut(s) 791
CseI GACGC 1 cut(s) 623
Csp6I GTAC 3 cut(s) 495, 571, 586
CviAII CATG 1 cut(s) 492
CviQI GTAC 3 cut(s) 495, 571, 586
DdeI CTNAG 5 cut(s) 14, 98, 273, 387, 648
DpnI GATC 1 cut(s) 798
DpnII GATC 1 cut(s) 796
DriI GACNNNNNGTC 1 cut(s) 187
Eam1105I GACNNNNNGTC 1 cut(s) 187
Eco130I CCWWGG 1 cut(s) 731
Eco147I AGGCCT 1 cut(s) 392
Eco57I CTGAAG 1 cut(s) 419
Eco88I CYCGRG 1 cut(s) 830
EcoRII CCWGG 1 cut(s) 525
EcoT14I CCWWGG 1 cut(s) 731
ErhI CCWWGG 1 cut(s) 731
Esp3I CGTCTC 1 cut(s) 606
FaeI CATG 1 cut(s) 495
FaqI GGGAC 1 cut(s) 605
FatI CATG 1 cut(s) 491
FauI CCCGC 1 cut(s) 210
Fnu4HI GCNGC 4 cut(s) 531, 750, 852, 855
FokI GGATG 2 cut(s) 251, 508
Fsp4HI GCNGC 4 cut(s) 531, 750, 852, 855
FspBI CTAG 3 cut(s) 278, 347, 689
GlaI GCGC 2 cut(s) 720, 858
GluI GCNGC 4 cut(s) 531, 750, 852, 855
HaeIII GGCC 3 cut(s) 392, 530, 851
HapII CCGG 2 cut(s) 792, 905
HgaI GACGC 1 cut(s) 623
HhaI GCGC 2 cut(s) 721, 859
Hin1II CATG 1 cut(s) 495
Hin6I GCGC 2 cut(s) 719, 857
HinP1I GCGC 2 cut(s) 719, 857
HincII GTYRAC 1 cut(s) 327
HindII GTYRAC 1 cut(s) 327
HindIII AAGCTT 1 cut(s) 444
HinfI GANTC 3 cut(s) 23, 78, 769
HpaII CCGG 2 cut(s) 792, 905
HphI GGTGA 3 cut(s) 275, 362, 806
Hpy166II GTNNAC 2 cut(s) 65, 327
Hpy188I TCNGA 3 cut(s) 83, 101, 202
Hpy188III TCNNGA 3 cut(s) 609, 689, 905
Hpy8I GTNNAC 2 cut(s) 65, 327
HpyAV CCTTC 7 cut(s) 20, 37, 43, 56, 408, 440, 911
HpyCH4III ACNGT 2 cut(s) 88, 407
HpyCH4IV ACGT 1 cut(s) 141
HpyCH4V TGCA 6 cut(s) 131, 148, 362, 397, 491, 892
HpyF10VI GCNNNNNNNGC 2 cut(s) 283, 826
HpyF3I CTNAG 5 cut(s) 14, 98, 273, 387, 648
HpySE526I ACGT 1 cut(s) 141
Hsp92II CATG 1 cut(s) 495
HspAI GCGC 2 cut(s) 719, 857
Kpn2I TCCGGA 1 cut(s) 904
Kzo9I GATC 1 cut(s) 796
Lsp1109I GCAGC 1 cut(s) 841
LweI GCATC 2 cut(s) 229, 292
MaeI CTAG 3 cut(s) 278, 347, 689
MaeII ACGT 1 cut(s) 141
MaeIII GTNAC 3 cut(s) 289, 350, 407
MalI GATC 1 cut(s) 798
MboI GATC 1 cut(s) 796
MboII GAAGA 4 cut(s) 425, 655, 896, 926
MfeI CAATTG 1 cut(s) 170
MluCI AATT 8 cut(s) 170, 229, 257, 320, 458, 627, 654, 893
MlyI GAGTC 1 cut(s) 32
MroI TCCGGA 1 cut(s) 904
MseI TTAA 3 cut(s) 461, 509, 576
MspA1I CMGCKG 2 cut(s) 749, 854
MspI CCGG 2 cut(s) 792, 905
MspR9I CCNGG 1 cut(s) 527
MunI CAATTG 1 cut(s) 170
MvaI CCWGG 1 cut(s) 527
MwoI GCNNNNNNNGC 2 cut(s) 283, 826
NdeII GATC 1 cut(s) 796
NheI GCTAGC 1 cut(s) 277
NlaIII CATG 1 cut(s) 495
NlaIV GGNNCC 1 cut(s) 253
NmuCI GTSAC 3 cut(s) 289, 350, 407
NspI RCATGY 1 cut(s) 495
PceI AGGCCT 1 cut(s) 392
PfeI GAWTC 2 cut(s) 78, 769
PflFI GACNNNGTC 1 cut(s) 617
PflMI CCANNNNNTGG 1 cut(s) 339
PkrI GCNGC 4 cut(s) 532, 751, 853, 856
PleI GAGTC 1 cut(s) 31
PpsI GAGTC 1 cut(s) 31
PshBI ATTAAT 1 cut(s) 461
Psp6I CCWGG 1 cut(s) 525
PspGI CCWGG 1 cut(s) 525
PspN4I GGNNCC 1 cut(s) 253
PsrI GAACNNNNNNTAC 2 cut(s) 478, 510
PstI CTGCAG 1 cut(s) 150
PsyI GACNNNGTC 1 cut(s) 617
RsaI GTAC 3 cut(s) 496, 572, 587
RsaNI GTAC 3 cut(s) 495, 571, 586
SaqAI TTAA 3 cut(s) 461, 509, 576
SatI GCNGC 4 cut(s) 531, 750, 852, 855
Sau3AI GATC 1 cut(s) 796
SchI GAGTC 1 cut(s) 32
ScrFI CCNGG 1 cut(s) 527
SfaNI GCATC 2 cut(s) 229, 292
SfcI CTRYAG 1 cut(s) 146
Sse9I AATT 8 cut(s) 170, 229, 257, 320, 458, 627, 654, 893
SseBI AGGCCT 1 cut(s) 392
SsiI CCGC 4 cut(s) 217, 531, 749, 852
SspMI CTAG 3 cut(s) 278, 347, 689
StuI AGGCCT 1 cut(s) 392
StyD4I CCNGG 1 cut(s) 525
StyI CCWWGG 1 cut(s) 731
TaaI ACNGT 2 cut(s) 88, 407
TaiI ACGT 1 cut(s) 144
TaqI TCGA 1 cut(s) 76
TasI AATT 8 cut(s) 170, 229, 257, 320, 458, 627, 654, 893
TatI WGTACW 1 cut(s) 494
TauI GCSGC 3 cut(s) 533, 752, 854
TfiI GAWTC 2 cut(s) 78, 769
Tru1I TTAA 3 cut(s) 461, 509, 576
Tru9I TTAA 3 cut(s) 461, 509, 576
TscAI CASTG 2 cut(s) 298, 412
TseFI GTSAC 3 cut(s) 289, 350, 407
TseI GCWGC 1 cut(s) 854
Tsp45I GTSAC 3 cut(s) 289, 350, 407
TspDTI ATGAA 3 cut(s) 456, 714, 927
TspRI CASTG 2 cut(s) 298, 412
Tth111I GACNNNGTC 1 cut(s) 617
Van91I CCANNNNNTGG 1 cut(s) 339
VspI ATTAAT 1 cut(s) 461
XapI RAATTY 2 cut(s) 257, 627
XbaI TCTAGA 1 cut(s) 688
XceI RCATGY 1 cut(s) 495
XspI CTAG 3 cut(s) 278, 347, 689
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.