Rh5DG322000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0005470
Physical Location & Seq
Reverse (-)
41849135 .. 41855520
6386 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG322000.1

Sequence Viewer

Length: 924 bp
ATGGAGACCCTTTTCAGGGTTGTGTGCTCCTCATTCCCAGCAACTCCTCCAACCCCTCATCGCTCACTTTCCACTAGAAACCCATCTGTCTCGGCCCTCAAAACAGGCCCATCTCCTGTGAGTGAGCTTGCAGAAGAGGACGTGCTGCAGACGTTCTTCAAGGAAAGACAGTTGAATGGAGACTTCATATCAAGACTTTCTGACGTGTTTTGGCAGAGGGAGTTTACGAATTTTGTTGATGGTGATGGTGTTGGTGACATTGATAACACTACTACTCAACAACAAGCACAAGAGCAGGCTGTGAATGACGCTGGTGGGTTTTTGAAACTGTCAGCAACCAATGAATGGGTGTTAGGTGATAGATCTGCACCGATTAATAAAAAAGCCATTGCTAAGGCCATGCAGAATGACAGTGAAAGAAGGAAGAAACTCAACCTTCTTCAATATGAAGCTATACAGAGGGAACTAATGCTTTTGTCTATTGGTATTGGAACTGCTTGTACTGGGTATTGTTTAATAACACTGTCATTCCAGGGTGCCGTCAGTTATGCGACAGGAGTTCTTTTCAGTTGCTTGTACCTTAAACTCTTGTACCAGCATGTAGACAATATATCCCGAGAAGCAGTCCCTCAGATTTTCCGGCAGAAGAAGTCTAAGAAAATTGGAATAAGAAGTCAGGACCTGGAGGATTTTCTAGAGAAATTGATAAAGGGCTGTGGTATGGCTCTTTCATCTCCAAGGCTTGTGTTACCAGCAGCAATATATGGATTGTGGATCCTGTCTCATCAACATGTTTCCAGTGATCTCTTTGATTTTCAGCTTGTGCCAGCTATGGTCGGGTTGTTTGTTTATAAAGCTGCTGCTCTTGTTCAAGTCTATAGAGATAATGAAAACCTGCAATTGATTTTTCCTGATGACCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

307

Amino Acids

34.25

Weight (kDa)

6.13

Isoelectric Point (pI)

40.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CGL160_C PF24763 148 - 209 3e-07 CGL160, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 852
Acc36I ACCTGC 1 cut(s) 903
AccB1I GGYRCC 1 cut(s) 536
AccB7I CCANNNNNTGG 1 cut(s) 345
AccI GTMKAC 1 cut(s) 603
AclWI GGATC 2 cut(s) 769, 782
AcsI RAATTY 1 cut(s) 229
AfaI GTAC 3 cut(s) 502, 578, 593
AfiI CCNNNNNNNGG 3 cut(s) 15, 16, 345
AflIII ACRYGT 2 cut(s) 204, 790
AgsI TTSAA 5 cut(s) 160, 175, 325, 443, 872
AjiI CACGTC 2 cut(s) 142, 205
AjnI CCWGG 2 cut(s) 531, 681
AluBI AGCT 5 cut(s) 127, 452, 820, 830, 857
AluI AGCT 5 cut(s) 127, 452, 820, 830, 857
Alw21I GWGCWC 1 cut(s) 29
Alw26I GTCTC 3 cut(s) 94, 174, 786
AlwI GGATC 2 cut(s) 769, 782
AlwNI CAGNNNCTG 1 cut(s) 682
Ama87I CYCGRG 1 cut(s) 615
AoxI GGCC 3 cut(s) 93, 106, 396
ApeKI GCWGC 4 cut(s) 145, 755, 857, 860
ApoI RAATTY 1 cut(s) 229
AseI ATTAAT 1 cut(s) 375
AspS9I GGNCC 3 cut(s) 94, 107, 679
AsuHPI GGTGA 3 cut(s) 254, 266, 368
AvaI CYCGRG 1 cut(s) 615
AvaII GGWCC 1 cut(s) 679
BaeI ACNNNNGTAYC 2 cut(s) 575, 608
BamHI GGATCC 1 cut(s) 774
BanI GGYRCC 1 cut(s) 536
Bbv12I GWGCWC 1 cut(s) 29
BbvI GCAGC 4 cut(s) 132, 767, 844, 847
BccI CCATC 4 cut(s) 91, 118, 233, 239
BceAI ACGGC 1 cut(s) 524
BciT130I CCWGG 2 cut(s) 533, 683
BcoDI GTCTC 3 cut(s) 94, 174, 786
BfaI CTAG 2 cut(s) 75, 695
BfmI CTRYAG 2 cut(s) 146, 877
BfuAI ACCTGC 1 cut(s) 903
BglII AGATCT 1 cut(s) 362
BisI GCNGC 4 cut(s) 146, 756, 858, 861
BlsI GCNGC 4 cut(s) 147, 757, 859, 862
Bme1390I CCNGG 2 cut(s) 533, 683
Bme18I GGWCC 1 cut(s) 679
BmeT110I CYCGRG 1 cut(s) 615
BmgBI CACGTC 2 cut(s) 142, 205
BmgT120I GGNCC 3 cut(s) 94, 107, 679
BmiI GGNNCC 2 cut(s) 538, 776
BmrFI CCNGG 2 cut(s) 533, 683
BmrI ACTGGG 1 cut(s) 513
BmuI ACTGGG 1 cut(s) 513
BpmI CTGGAG 1 cut(s) 704
Bpu10I CCTNAGC 1 cut(s) 393
BsaJI CCNNGG 2 cut(s) 532, 737
Bsc4I CCNNNNNNNGG 3 cut(s) 15, 16, 345
Bse1I ACTGG 2 cut(s) 508, 798
Bse3DI GCAATG 1 cut(s) 387
BseBI CCWGG 2 cut(s) 533, 683
BseDI CCNNGG 2 cut(s) 532, 737
BseLI CCNNNNNNNGG 3 cut(s) 15, 16, 345
BseMI GCAATG 1 cut(s) 387
BseMII CTCAG 1 cut(s) 644
BseNI ACTGG 2 cut(s) 508, 798
BseRI GAGGAG 2 cut(s) 19, 36
BseXI GCAGC 4 cut(s) 132, 767, 844, 847
BseYI CCCAGC 1 cut(s) 37
BsgI GTGCAG 1 cut(s) 351
BshFI GGCC 3 cut(s) 95, 108, 398
BshNI GGYRCC 1 cut(s) 536
BsiHKAI GWGCWC 1 cut(s) 29
BsiHKCI CYCGRG 1 cut(s) 615
BsiSI CCGG 1 cut(s) 640
BslFI GGGAC 1 cut(s) 611
BslI CCNNNNNNNGG 3 cut(s) 15, 16, 345
BsmAI GTCTC 3 cut(s) 94, 174, 786
BsmFI GGGAC 1 cut(s) 611
BsnI GGCC 3 cut(s) 95, 108, 398
BsoBI CYCGRG 1 cut(s) 615
Bsp1286I GDGCHC 1 cut(s) 29
Bsp143I GATC 3 cut(s) 362, 774, 802
BspANI GGCC 3 cut(s) 95, 108, 398
BspCNI CTCAG 1 cut(s) 643
BspLI GGNNCC 2 cut(s) 538, 776
BspMAI CTGCAG 1 cut(s) 150
BspMI ACCTGC 1 cut(s) 903
BspPI GGATC 2 cut(s) 769, 782
BspT107I GGYRCC 1 cut(s) 536
BsrDI GCAATG 1 cut(s) 387
BsrI ACTGG 2 cut(s) 508, 798
BssECI CCNNGG 2 cut(s) 532, 737
BssMI GATC 3 cut(s) 362, 774, 802
BssT1I CCWWGG 1 cut(s) 737
Bst2UI CCWGG 2 cut(s) 533, 683
Bst4CI ACNGT 4 cut(s) 171, 330, 413, 525
Bst6I CTCTTC 1 cut(s) 129
BstC8I GCNNGC 3 cut(s) 129, 297, 828
BstDEI CTNAG 3 cut(s) 393, 630, 654
BstKTI GATC 3 cut(s) 365, 777, 805
BstMAI GTCTC 3 cut(s) 94, 174, 786
BstMBI GATC 3 cut(s) 362, 774, 802
BstNI CCWGG 2 cut(s) 533, 683
BstNSI RCATGY 2 cut(s) 602, 794
BstSCI CCNGG 2 cut(s) 531, 681
BstSFI CTRYAG 2 cut(s) 146, 877
BstV1I GCAGC 4 cut(s) 132, 767, 844, 847
BstX2I RGATCY 2 cut(s) 362, 774
BstYI RGATCY 2 cut(s) 362, 774
BsuRI GGCC 3 cut(s) 95, 108, 398
BtgZI GCGATG 1 cut(s) 44
BtrI CACGTC 2 cut(s) 142, 205
BtsIMutI CAGTG 3 cut(s) 418, 521, 805
BveI ACCTGC 1 cut(s) 903
Cac8I GCNNGC 3 cut(s) 129, 297, 828
CaiI CAGNNNCTG 1 cut(s) 682
Cfr13I GGNCC 3 cut(s) 94, 107, 679
CseI GACGC 1 cut(s) 317
Csp6I GTAC 3 cut(s) 501, 577, 592
CviAII CATG 3 cut(s) 400, 599, 791
CviQI GTAC 3 cut(s) 501, 577, 592
DdeI CTNAG 3 cut(s) 393, 630, 654
DpnI GATC 3 cut(s) 364, 776, 804
DpnII GATC 3 cut(s) 362, 774, 802
Eam1104I CTCTTC 1 cut(s) 129
EarI CTCTTC 1 cut(s) 129
Eco130I CCWWGG 1 cut(s) 737
Eco47I GGWCC 1 cut(s) 679
Eco88I CYCGRG 1 cut(s) 615
EcoO109I RGGNCCY 1 cut(s) 679
EcoRII CCWGG 2 cut(s) 531, 681
EcoT14I CCWWGG 1 cut(s) 737
ErhI CCWWGG 1 cut(s) 737
FaeI CATG 3 cut(s) 403, 602, 794
FaqI GGGAC 1 cut(s) 611
FatI CATG 3 cut(s) 399, 598, 790
FblI GTMKAC 1 cut(s) 603
Fnu4HI GCNGC 4 cut(s) 146, 756, 858, 861
Fsp4HI GCNGC 4 cut(s) 146, 756, 858, 861
FspBI CTAG 2 cut(s) 75, 695
GluI GCNGC 4 cut(s) 146, 756, 858, 861
GsaI CCCAGC 1 cut(s) 41
GsuI CTGGAG 1 cut(s) 704
HaeIII GGCC 3 cut(s) 95, 108, 398
HapII CCGG 1 cut(s) 640
HgaI GACGC 1 cut(s) 317
Hin1II CATG 3 cut(s) 403, 602, 794
HpaII CCGG 1 cut(s) 640
HphI GGTGA 3 cut(s) 254, 266, 368
Hpy166II GTNNAC 2 cut(s) 225, 604
Hpy188I TCNGA 2 cut(s) 202, 633
Hpy188III TCNNGA 5 cut(s) 192, 615, 677, 695, 911
Hpy8I GTNNAC 2 cut(s) 225, 604
HpyAV CCTTC 2 cut(s) 414, 446
HpyCH4III ACNGT 4 cut(s) 171, 330, 413, 525
HpyCH4IV ACGT 3 cut(s) 141, 152, 204
HpyCH4V TGCA 5 cut(s) 131, 148, 368, 403, 898
HpyF3I CTNAG 3 cut(s) 393, 630, 654
HpySE526I ACGT 3 cut(s) 141, 152, 204
Hsp92II CATG 3 cut(s) 403, 602, 794
Kzo9I GATC 3 cut(s) 362, 774, 802
LmnI GCTCC 1 cut(s) 32
Lsp1109I GCAGC 4 cut(s) 132, 767, 844, 847
MaeI CTAG 2 cut(s) 75, 695
MaeII ACGT 3 cut(s) 141, 152, 204
MaeIII GTNAC 2 cut(s) 254, 747
MalI GATC 3 cut(s) 364, 776, 804
MboI GATC 3 cut(s) 362, 774, 802
MboII GAAGA 5 cut(s) 146, 148, 431, 436, 658
MfeI CAATTG 1 cut(s) 899
MflI RGATCY 2 cut(s) 362, 774
MhlI GDGCHC 1 cut(s) 29
MluCI AATT 4 cut(s) 229, 660, 701, 899
MmeI TCCRAC 1 cut(s) 74
MnlI CCTC 9 cut(s) 40, 57, 66, 107, 130, 210, 453, 639, 679
MseI TTAA 3 cut(s) 375, 515, 582
MslI CAYNNNNRTG 1 cut(s) 789
MspI CCGG 1 cut(s) 640
MspR9I CCNGG 2 cut(s) 533, 683
MunI CAATTG 1 cut(s) 899
MvaI CCWGG 2 cut(s) 533, 683
NdeII GATC 3 cut(s) 362, 774, 802
NlaIII CATG 3 cut(s) 403, 602, 794
NlaIV GGNNCC 2 cut(s) 538, 776
NmeAIII GCCGAG 1 cut(s) 71
NmuCI GTSAC 1 cut(s) 254
NspI RCATGY 2 cut(s) 602, 794
PciI ACATGT 1 cut(s) 790
PflMI CCANNNNNTGG 1 cut(s) 345
PkrI GCNGC 4 cut(s) 147, 757, 859, 862
PpuMI RGGWCCY 1 cut(s) 679
PscI ACATGT 1 cut(s) 790
PshBI ATTAAT 1 cut(s) 375
PsiI TTATAA 1 cut(s) 852
Psp5II RGGWCCY 1 cut(s) 679
Psp6I CCWGG 2 cut(s) 531, 681
PspFI CCCAGC 1 cut(s) 37
PspGI CCWGG 2 cut(s) 531, 681
PspN4I GGNNCC 2 cut(s) 538, 776
PspPI GGNCC 3 cut(s) 94, 107, 679
PspPPI RGGWCCY 1 cut(s) 679
PsrI GAACNNNNNNTAC 2 cut(s) 484, 516
PstI CTGCAG 1 cut(s) 150
PstNI CAGNNNCTG 1 cut(s) 682
PsuI RGATCY 2 cut(s) 362, 774
RsaI GTAC 3 cut(s) 502, 578, 593
RsaNI GTAC 3 cut(s) 501, 577, 592
RseI CAYNNNNRTG 1 cut(s) 789
SaqAI TTAA 3 cut(s) 375, 515, 582
SatI GCNGC 4 cut(s) 146, 756, 858, 861
Sau3AI GATC 3 cut(s) 362, 774, 802
Sau96I GGNCC 3 cut(s) 94, 107, 679
ScrFI CCNGG 2 cut(s) 533, 683
SduI GDGCHC 1 cut(s) 29
SfcI CTRYAG 2 cut(s) 146, 877
SinI GGWCC 1 cut(s) 679
SmiMI CAYNNNNRTG 1 cut(s) 789
Sse9I AATT 4 cut(s) 229, 660, 701, 899
SspMI CTAG 2 cut(s) 75, 695
StyD4I CCNGG 2 cut(s) 531, 681
StyI CCWWGG 1 cut(s) 737
TaaI ACNGT 4 cut(s) 171, 330, 413, 525
TaiI ACGT 3 cut(s) 144, 155, 207
TasI AATT 4 cut(s) 229, 660, 701, 899
TatI WGTACW 1 cut(s) 500
Tru1I TTAA 3 cut(s) 375, 515, 582
Tru9I TTAA 3 cut(s) 375, 515, 582
TscAI CASTG 3 cut(s) 418, 528, 805
TseFI GTSAC 1 cut(s) 254
TseI GCWGC 4 cut(s) 145, 755, 857, 860
Tsp45I GTSAC 1 cut(s) 254
TspDTI ATGAA 5 cut(s) 175, 357, 462, 720, 903
TspRI CASTG 3 cut(s) 418, 528, 805
Van91I CCANNNNNTGG 1 cut(s) 345
VpaK11BI GGWCC 1 cut(s) 679
VspI ATTAAT 1 cut(s) 375
XapI RAATTY 1 cut(s) 229
XbaI TCTAGA 1 cut(s) 694
XceI RCATGY 2 cut(s) 602, 794
XmiI GTMKAC 1 cut(s) 603
XspI CTAG 2 cut(s) 75, 695
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.