MD03G1149900.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0005470
Physical Location & Seq
Reverse (-)
16670732 .. 16677212
6481 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1149900.v1.1.491

Sequence Viewer

Length: 930 bp
ATGGAGACCCTTCTCAGGGTTGAGTCCTTCTCCTTCCCAAGAACCCTTCCAATCCCTAATCGTTCACTTCCCAATCGAATCCCACCTGTCTCTGCCCTCAGAACAACCCCACCTCCTGTGAGTGAAGTTGCAGAGGAGGACGTTCTGCAGACATTTTTCAAGGAAAGGCAATTGAATGGAGACTTGGTGTCAAAAGTTTCTGATGTATTTTGGAAGCGGGAGTTTGCCAATTCTATTGATACTGATGATGGTGCCGAATTTGGTGATACTCCTCAGCTAGAAGAGCAGGTCACTGAAAATGATGCTGGTGGATTTTTGAAATTGTCAACAACCAACGAGTGGGTTCTAGGTGACATATCTGCACCAGTAAATAGAAAAGCCAGAGCTAAGGCCTTGCAGAATGACAGTGACAGAAGGAGGAAACTCAACCTTCTTCAGTATGAAGCTTTACAGAGGGAATTAATGCTGTTATCCATTGGTATTGGAACTGCATGTACTGGGTATTGCTTAATAGCATTGTCATCCCAGGCCGCTGTCAGTTATGCCACGGGAGTTCTTTTCAGTTGCTTGTACCTTAAACTCTTGTACCAGCAAGCAGACAATATATCCAGAGACGCAGTCCCTCAAATTTTCAGGCAGAGGAAGACTAAGAAAATTGGAATAAGAACCGAGGATTTGGAGGATTTTCTAGAGAAAACGATAAAGGGGTGTGGTATTGCGCTTTCATCTCCAAGGCTTGTAATACCAGCGGCAATATATGGATTGTGGATTCTGTCTCATCAAAAGTTTGCAGGTGATTTGTTTGATTTCCAGCTTGTACCAGCTATGCTTGGGTTGTTTGTGTATAAAGCCGCTGCACTATATCAAGTTTATAGAGATAACGAAGACTTGCAATTTATCTTTCCGGATAATGAAGAAGGCTCAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

310

Amino Acids

34.68

Weight (kDa)

5.22

Isoelectric Point (pI)

45.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CGL160_C PF24763 146 - 208 8.9e-08 CGL160, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 782
Acc36I ACCTGC 2 cut(s) 277, 782
AccB1I GGYRCC 1 cut(s) 251
AccB7I CCANNNNNTGG 1 cut(s) 339
AccIII TCCGGA 1 cut(s) 904
AciI CCGC 4 cut(s) 217, 531, 749, 852
AcsI RAATTY 2 cut(s) 257, 627
AcuI CTGAAG 1 cut(s) 419
AfaI GTAC 4 cut(s) 496, 572, 587, 819
AfiI CCNNNNNNNGG 3 cut(s) 15, 16, 339
AgsI TTSAA 3 cut(s) 160, 175, 319
AhdI GACNNNNNGTC 1 cut(s) 187
AjnI CCWGG 1 cut(s) 525
AjuI GAANNNNNNNTTGG 2 cut(s) 167, 199
AluBI AGCT 5 cut(s) 277, 386, 446, 814, 824
AluI AGCT 5 cut(s) 277, 386, 446, 814, 824
Alw26I GTCTC 4 cut(s) 94, 174, 606, 780
Aor13HI TCCGGA 1 cut(s) 904
AoxI GGCC 2 cut(s) 390, 528
ApeKI GCWGC 1 cut(s) 854
ApoI RAATTY 2 cut(s) 257, 627
AseI ATTAAT 1 cut(s) 461
AspLEI GCGC 1 cut(s) 721
AsuHPI GGTGA 3 cut(s) 275, 362, 806
BaeI ACNNNNGTAYC 2 cut(s) 569, 602
BanI GGYRCC 1 cut(s) 251
BbsI GAAGAC 2 cut(s) 650, 891
BbvCI CCTCAGC 1 cut(s) 273
BbvI GCAGC 1 cut(s) 841
BccI CCATC 1 cut(s) 242
BciT130I CCWGG 1 cut(s) 527
BcoDI GTCTC 4 cut(s) 94, 174, 606, 780
BfaI CTAG 3 cut(s) 278, 347, 689
BfmI CTRYAG 1 cut(s) 146
BfuAI ACCTGC 2 cut(s) 277, 782
BisI GCNGC 4 cut(s) 531, 750, 852, 855
BlsI GCNGC 4 cut(s) 532, 751, 853, 856
Bme1390I CCNGG 1 cut(s) 527
BmeRI GACNNNNNGTC 1 cut(s) 187
BmiI GGNNCC 1 cut(s) 253
BmrFI CCNGG 1 cut(s) 527
BmrI ACTGGG 1 cut(s) 507
BmsI GCATC 1 cut(s) 292
BmuI ACTGGG 1 cut(s) 507
BpiI GAAGAC 2 cut(s) 650, 891
BplI GAGNNNNNCTC 2 cut(s) 14, 46
Bpu10I CCTNAGC 2 cut(s) 273, 387
BsaJI CCNNGG 4 cut(s) 525, 546, 669, 731
BsaWI WCCGGW 1 cut(s) 904
BsaXI ACNNNNNCTCC 4 cut(s) 97, 127, 171, 201
Bsc4I CCNNNNNNNGG 3 cut(s) 15, 16, 339
Bse1I ACTGG 2 cut(s) 365, 502
BseAI TCCGGA 1 cut(s) 904
BseBI CCWGG 1 cut(s) 527
BseDI CCNNGG 4 cut(s) 525, 546, 669, 731
BseGI GGATG 1 cut(s) 521
BseLI CCNNNNNNNGG 3 cut(s) 15, 16, 339
BseMII CTCAG 3 cut(s) 28, 112, 287
BseNI ACTGG 2 cut(s) 365, 502
BseRI GAGGAG 2 cut(s) 149, 261
BseXI GCAGC 1 cut(s) 841
BsgI GTGCAG 2 cut(s) 345, 840
BshFI GGCC 2 cut(s) 392, 530
BshNI GGYRCC 1 cut(s) 251
BsiSI CCGG 1 cut(s) 905
BslFI GGGAC 1 cut(s) 605
BslI CCNNNNNNNGG 3 cut(s) 15, 16, 339
BsmAI GTCTC 4 cut(s) 94, 174, 606, 780
BsmBI CGTCTC 1 cut(s) 606
BsmFI GGGAC 1 cut(s) 605
BsnI GGCC 2 cut(s) 392, 530
Bsp13I TCCGGA 1 cut(s) 904
BspACI CCGC 4 cut(s) 217, 531, 749, 852
BspANI GGCC 2 cut(s) 392, 530
BspCNI CTCAG 3 cut(s) 27, 111, 286
BspEI TCCGGA 1 cut(s) 904
BspLI GGNNCC 1 cut(s) 253
BspMAI CTGCAG 1 cut(s) 150
BspMI ACCTGC 2 cut(s) 277, 782
BspQI GCTCTTC 1 cut(s) 276
BspT107I GGYRCC 1 cut(s) 251
BsrI ACTGG 2 cut(s) 365, 502
BssECI CCNNGG 4 cut(s) 525, 546, 669, 731
BssT1I CCWWGG 1 cut(s) 731
Bst2UI CCWGG 1 cut(s) 527
Bst4CI ACNGT 1 cut(s) 407
Bst6I CTCTTC 1 cut(s) 276
BstC8I GCNNGC 1 cut(s) 594
BstDEI CTNAG 5 cut(s) 14, 98, 273, 387, 648
BstDSI CCRYGG 1 cut(s) 546
BstF5I GGATG 1 cut(s) 521
BstHHI GCGC 1 cut(s) 721
BstMAI GTCTC 4 cut(s) 94, 174, 606, 780
BstMWI GCNNNNNNNGC 1 cut(s) 283
BstNI CCWGG 1 cut(s) 527
BstNSI RCATGY 1 cut(s) 495
BstSCI CCNGG 1 cut(s) 525
BstSFI CTRYAG 1 cut(s) 146
BstV1I GCAGC 1 cut(s) 841
BstV2I GAAGAC 2 cut(s) 650, 891
BsuRI GGCC 2 cut(s) 392, 530
BtgI CCRYGG 1 cut(s) 546
BtsCI GGATG 1 cut(s) 521
BtsIMutI CAGTG 2 cut(s) 291, 412
BveI ACCTGC 2 cut(s) 277, 782
Cac8I GCNNGC 1 cut(s) 594
CfoI GCGC 1 cut(s) 721
CseI GACGC 1 cut(s) 623
Csp6I GTAC 4 cut(s) 495, 571, 586, 818
CviAII CATG 1 cut(s) 492
CviQI GTAC 4 cut(s) 495, 571, 586, 818
DdeI CTNAG 5 cut(s) 14, 98, 273, 387, 648
DriI GACNNNNNGTC 1 cut(s) 187
Eam1104I CTCTTC 1 cut(s) 276
Eam1105I GACNNNNNGTC 1 cut(s) 187
EarI CTCTTC 1 cut(s) 276
Eco130I CCWWGG 1 cut(s) 731
Eco147I AGGCCT 1 cut(s) 392
Eco57I CTGAAG 1 cut(s) 419
EcoRII CCWGG 1 cut(s) 525
EcoT14I CCWWGG 1 cut(s) 731
ErhI CCWWGG 1 cut(s) 731
Esp3I CGTCTC 1 cut(s) 606
FaeI CATG 1 cut(s) 495
FaqI GGGAC 1 cut(s) 605
FatI CATG 1 cut(s) 491
FauI CCCGC 1 cut(s) 210
Fnu4HI GCNGC 4 cut(s) 531, 750, 852, 855
FokI GGATG 1 cut(s) 508
Fsp4HI GCNGC 4 cut(s) 531, 750, 852, 855
FspBI CTAG 3 cut(s) 278, 347, 689
GlaI GCGC 1 cut(s) 720
GluI GCNGC 4 cut(s) 531, 750, 852, 855
HaeIII GGCC 2 cut(s) 392, 530
HapII CCGG 1 cut(s) 905
HgaI GACGC 1 cut(s) 623
HhaI GCGC 1 cut(s) 721
Hin1II CATG 1 cut(s) 495
Hin6I GCGC 1 cut(s) 719
HinP1I GCGC 1 cut(s) 719
HincII GTYRAC 1 cut(s) 327
HindII GTYRAC 1 cut(s) 327
HindIII AAGCTT 1 cut(s) 444
HinfI GANTC 3 cut(s) 23, 78, 769
HpaII CCGG 1 cut(s) 905
HphI GGTGA 3 cut(s) 275, 362, 806
Hpy166II GTNNAC 2 cut(s) 65, 327
Hpy188I TCNGA 2 cut(s) 101, 202
Hpy188III TCNNGA 3 cut(s) 609, 689, 905
Hpy8I GTNNAC 2 cut(s) 65, 327
HpyAV CCTTC 7 cut(s) 20, 37, 43, 56, 408, 440, 911
HpyCH4III ACNGT 1 cut(s) 407
HpyCH4IV ACGT 1 cut(s) 141
HpyCH4V TGCA 8 cut(s) 131, 148, 362, 397, 491, 791, 857, 892
HpyF10VI GCNNNNNNNGC 1 cut(s) 283
HpyF3I CTNAG 5 cut(s) 14, 98, 273, 387, 648
HpySE526I ACGT 1 cut(s) 141
Hsp92II CATG 1 cut(s) 495
HspAI GCGC 1 cut(s) 719
Kpn2I TCCGGA 1 cut(s) 904
LguI GCTCTTC 1 cut(s) 276
Lsp1109I GCAGC 1 cut(s) 841
LweI GCATC 1 cut(s) 292
MaeI CTAG 3 cut(s) 278, 347, 689
MaeII ACGT 1 cut(s) 141
MaeIII GTNAC 3 cut(s) 289, 350, 407
MboII GAAGA 5 cut(s) 293, 425, 655, 896, 926
MfeI CAATTG 1 cut(s) 170
MluCI AATT 8 cut(s) 170, 229, 257, 320, 458, 627, 654, 893
MlyI GAGTC 1 cut(s) 32
MroI TCCGGA 1 cut(s) 904
MseI TTAA 3 cut(s) 461, 509, 576
MspA1I CMGCKG 3 cut(s) 533, 749, 854
MspI CCGG 1 cut(s) 905
MspR9I CCNGG 1 cut(s) 527
MunI CAATTG 1 cut(s) 170
MvaI CCWGG 1 cut(s) 527
MwoI GCNNNNNNNGC 1 cut(s) 283
NlaIII CATG 1 cut(s) 495
NlaIV GGNNCC 1 cut(s) 253
NmuCI GTSAC 3 cut(s) 289, 350, 407
NspI RCATGY 1 cut(s) 495
PaqCI CACCTGC 1 cut(s) 782
PceI AGGCCT 1 cut(s) 392
PciSI GCTCTTC 1 cut(s) 276
PfeI GAWTC 2 cut(s) 78, 769
PflFI GACNNNGTC 1 cut(s) 617
PflMI CCANNNNNTGG 1 cut(s) 339
PkrI GCNGC 4 cut(s) 532, 751, 853, 856
PleI GAGTC 1 cut(s) 31
PpsI GAGTC 1 cut(s) 31
PshBI ATTAAT 1 cut(s) 461
Psp6I CCWGG 1 cut(s) 525
PspGI CCWGG 1 cut(s) 525
PspN4I GGNNCC 1 cut(s) 253
PsrI GAACNNNNNNTAC 2 cut(s) 478, 510
PstI CTGCAG 1 cut(s) 150
PsyI GACNNNGTC 1 cut(s) 617
RsaI GTAC 4 cut(s) 496, 572, 587, 819
RsaNI GTAC 4 cut(s) 495, 571, 586, 818
SapI GCTCTTC 1 cut(s) 276
SaqAI TTAA 3 cut(s) 461, 509, 576
SatI GCNGC 4 cut(s) 531, 750, 852, 855
SchI GAGTC 1 cut(s) 32
ScrFI CCNGG 1 cut(s) 527
SfaNI GCATC 1 cut(s) 292
SfcI CTRYAG 1 cut(s) 146
Sse9I AATT 8 cut(s) 170, 229, 257, 320, 458, 627, 654, 893
SseBI AGGCCT 1 cut(s) 392
SsiI CCGC 4 cut(s) 217, 531, 749, 852
SspMI CTAG 3 cut(s) 278, 347, 689
StuI AGGCCT 1 cut(s) 392
StyD4I CCNGG 1 cut(s) 525
StyI CCWWGG 1 cut(s) 731
TaaI ACNGT 1 cut(s) 407
TaiI ACGT 1 cut(s) 144
TaqI TCGA 1 cut(s) 76
TasI AATT 8 cut(s) 170, 229, 257, 320, 458, 627, 654, 893
TatI WGTACW 1 cut(s) 494
TauI GCSGC 3 cut(s) 533, 752, 854
TfiI GAWTC 2 cut(s) 78, 769
Tru1I TTAA 3 cut(s) 461, 509, 576
Tru9I TTAA 3 cut(s) 461, 509, 576
TscAI CASTG 2 cut(s) 298, 412
TseFI GTSAC 3 cut(s) 289, 350, 407
TseI GCWGC 1 cut(s) 854
Tsp45I GTSAC 3 cut(s) 289, 350, 407
TspDTI ATGAA 3 cut(s) 456, 714, 927
TspRI CASTG 2 cut(s) 298, 412
Tth111I GACNNNGTC 1 cut(s) 617
Van91I CCANNNNNTGG 1 cut(s) 339
VspI ATTAAT 1 cut(s) 461
XapI RAATTY 2 cut(s) 257, 627
XbaI TCTAGA 1 cut(s) 688
XceI RCATGY 1 cut(s) 495
XspI CTAG 3 cut(s) 278, 347, 689
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.