Rroxscaffold_1G00035790

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0005470
Physical Location & Seq
Forward (+)
52093197 .. 52099077
5881 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00035790.1

Sequence Viewer

Length: 924 bp
ATGGAGACCCTTTTCAGGGTTGTGTGCTCCTCATTCCCAGCAACTCCTCCAACCCCTCATCGCTCACTTTCCACTAGAAACCCATCTGTCTCGGCCCTCAAAACAGGCCCATCTCAGGTGAGTGAGCTTGCAGAAGAGGACGTGCTGCAGACGTTCTTCAAGGAAAGACAGTTGAATGGAGACTTCATATCAAGGCTTTCTGACGTGTTTTGGCAGAGGGAGTTTACGAATTTTGTTGATGGTGATGGTGTTGGTGACATTGATGGCACAACTACTCAACAACAAGCAGAAGAGCAGGCTGTGAATGACGCTGGTGGGTTTTTGAAACTGTCAGCAACCAATGAATGGGTGTTAGGTGATAGATCTGCACCGATTAATAAAAAAGCCATTGCTAAGGCCATGCAGAATGACAGTGAAAGAAGGAAGAAACTCAACCTTCTTCAATATGAAGCTATACAGAGGGAACTAATGCTTTTGTCTATTGGTATTGGAACTGCTTGTACTGGGTATTGTTTAATAACACTGTCATTCCAGGGTGCCGTCAGTTACGCGACAGGAGTTCTTTTCAGTTGCTTGTACCTTAAACTCTTGTACCAGCATGTAGACAATATATCCCAAGAAGCAGTCCCTCAGATTTTCCGGCAGAAGAAGTCTAAGAAAATTGGAATAAGAAGTCAGGATCTGGAGGATTTTCTAGAGAAATTGATAAAGGGCTGTGGTATGGCTCTTTCATCTCCAAGGCTTGTGTTACCAGCAGCAATATATGGATTGTGGATCCTGTCTCATCAATATGTTTCCAGTGATCTCTTTGATTTTCAGCTTGTGCCAGCTATGGTCGGGTTGTTTGTTTATAAAGCTGCTGCTCTTGTTCAAGTTTATAGAGATAATGAAGACCTGCAATTGATCTTTCCTGATGACCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

307

Amino Acids

34.22

Weight (kDa)

5.37

Isoelectric Point (pI)

40.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CGL160_C PF24763 148 - 210 1.3e-07 CGL160, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 852
Acc36I ACCTGC 1 cut(s) 903
AccB1I GGYRCC 1 cut(s) 536
AccB7I CCANNNNNTGG 1 cut(s) 345
AccI GTMKAC 1 cut(s) 603
AccII CGCG 1 cut(s) 551
AclWI GGATC 3 cut(s) 687, 769, 782
AcsI RAATTY 1 cut(s) 229
AfaI GTAC 3 cut(s) 502, 578, 593
AfiI CCNNNNNNNGG 4 cut(s) 15, 16, 115, 345
AflIII ACRYGT 1 cut(s) 204
AgsI TTSAA 5 cut(s) 160, 175, 325, 443, 872
AjiI CACGTC 2 cut(s) 142, 205
AjnI CCWGG 1 cut(s) 531
AluBI AGCT 5 cut(s) 127, 452, 820, 830, 857
AluI AGCT 5 cut(s) 127, 452, 820, 830, 857
Alw21I GWGCWC 1 cut(s) 29
Alw26I GTCTC 3 cut(s) 94, 174, 786
AlwI GGATC 3 cut(s) 687, 769, 782
AlwNI CAGNNNCTG 1 cut(s) 682
AoxI GGCC 3 cut(s) 93, 106, 396
ApeKI GCWGC 4 cut(s) 145, 755, 857, 860
ApoI RAATTY 1 cut(s) 229
AseI ATTAAT 1 cut(s) 375
AspS9I GGNCC 2 cut(s) 94, 107
AsuHPI GGTGA 4 cut(s) 130, 254, 266, 368
BaeI ACNNNNGTAYC 2 cut(s) 575, 608
BamHI GGATCC 1 cut(s) 774
BanI GGYRCC 1 cut(s) 536
BbsI GAAGAC 1 cut(s) 897
Bbv12I GWGCWC 1 cut(s) 29
BbvI GCAGC 4 cut(s) 132, 767, 844, 847
BccI CCATC 5 cut(s) 91, 118, 233, 239, 257
BceAI ACGGC 1 cut(s) 524
BciT130I CCWGG 1 cut(s) 533
BcoDI GTCTC 3 cut(s) 94, 174, 786
BfaI CTAG 2 cut(s) 75, 695
BfmI CTRYAG 1 cut(s) 146
BfuAI ACCTGC 1 cut(s) 903
BglII AGATCT 1 cut(s) 362
BisI GCNGC 4 cut(s) 146, 756, 858, 861
BlsI GCNGC 4 cut(s) 147, 757, 859, 862
Bme1390I CCNGG 1 cut(s) 533
BmgBI CACGTC 2 cut(s) 142, 205
BmgT120I GGNCC 2 cut(s) 94, 107
BmiI GGNNCC 2 cut(s) 538, 776
BmrFI CCNGG 1 cut(s) 533
BmrI ACTGGG 1 cut(s) 513
BmuI ACTGGG 1 cut(s) 513
BpiI GAAGAC 1 cut(s) 897
BpmI CTGGAG 1 cut(s) 704
Bpu10I CCTNAGC 1 cut(s) 393
BsaJI CCNNGG 2 cut(s) 532, 737
Bsc4I CCNNNNNNNGG 4 cut(s) 15, 16, 115, 345
Bse1I ACTGG 2 cut(s) 508, 798
Bse3DI GCAATG 1 cut(s) 387
BseBI CCWGG 1 cut(s) 533
BseDI CCNNGG 2 cut(s) 532, 737
BseLI CCNNNNNNNGG 4 cut(s) 15, 16, 115, 345
BseMI GCAATG 1 cut(s) 387
BseMII CTCAG 2 cut(s) 128, 644
BseNI ACTGG 2 cut(s) 508, 798
BseRI GAGGAG 2 cut(s) 19, 36
BseXI GCAGC 4 cut(s) 132, 767, 844, 847
BseYI CCCAGC 1 cut(s) 37
BsgI GTGCAG 1 cut(s) 351
Bsh1236I CGCG 1 cut(s) 551
BshFI GGCC 3 cut(s) 95, 108, 398
BshNI GGYRCC 1 cut(s) 536
BsiHKAI GWGCWC 1 cut(s) 29
BsiSI CCGG 1 cut(s) 640
BslFI GGGAC 1 cut(s) 611
BslI CCNNNNNNNGG 4 cut(s) 15, 16, 115, 345
BsmAI GTCTC 3 cut(s) 94, 174, 786
BsmFI GGGAC 1 cut(s) 611
BsnI GGCC 3 cut(s) 95, 108, 398
Bsp1286I GDGCHC 1 cut(s) 29
Bsp143I GATC 5 cut(s) 362, 679, 774, 802, 903
BspANI GGCC 3 cut(s) 95, 108, 398
BspCNI CTCAG 2 cut(s) 127, 643
BspFNI CGCG 1 cut(s) 551
BspLI GGNNCC 2 cut(s) 538, 776
BspMAI CTGCAG 1 cut(s) 150
BspMI ACCTGC 1 cut(s) 903
BspPI GGATC 3 cut(s) 687, 769, 782
BspQI GCTCTTC 1 cut(s) 285
BspT107I GGYRCC 1 cut(s) 536
BsrDI GCAATG 1 cut(s) 387
BsrI ACTGG 2 cut(s) 508, 798
BssECI CCNNGG 2 cut(s) 532, 737
BssMI GATC 5 cut(s) 362, 679, 774, 802, 903
BssT1I CCWWGG 1 cut(s) 737
Bst2UI CCWGG 1 cut(s) 533
Bst4CI ACNGT 4 cut(s) 171, 330, 413, 525
Bst6I CTCTTC 2 cut(s) 129, 285
BstC8I GCNNGC 3 cut(s) 129, 297, 828
BstDEI CTNAG 4 cut(s) 114, 393, 630, 654
BstFNI CGCG 1 cut(s) 551
BstKTI GATC 5 cut(s) 365, 682, 777, 805, 906
BstMAI GTCTC 3 cut(s) 94, 174, 786
BstMBI GATC 5 cut(s) 362, 679, 774, 802, 903
BstNI CCWGG 1 cut(s) 533
BstNSI RCATGY 1 cut(s) 602
BstSCI CCNGG 1 cut(s) 531
BstSFI CTRYAG 1 cut(s) 146
BstUI CGCG 1 cut(s) 551
BstV1I GCAGC 4 cut(s) 132, 767, 844, 847
BstV2I GAAGAC 1 cut(s) 897
BstX2I RGATCY 3 cut(s) 362, 679, 774
BstYI RGATCY 3 cut(s) 362, 679, 774
BsuRI GGCC 3 cut(s) 95, 108, 398
BtgZI GCGATG 1 cut(s) 44
BtrI CACGTC 2 cut(s) 142, 205
BtsIMutI CAGTG 3 cut(s) 418, 521, 805
BveI ACCTGC 1 cut(s) 903
Cac8I GCNNGC 3 cut(s) 129, 297, 828
CaiI CAGNNNCTG 1 cut(s) 682
Cfr13I GGNCC 2 cut(s) 94, 107
CseI GACGC 1 cut(s) 317
Csp6I GTAC 3 cut(s) 501, 577, 592
CviAII CATG 2 cut(s) 400, 599
CviQI GTAC 3 cut(s) 501, 577, 592
DdeI CTNAG 4 cut(s) 114, 393, 630, 654
DpnI GATC 5 cut(s) 364, 681, 776, 804, 905
DpnII GATC 5 cut(s) 362, 679, 774, 802, 903
Eam1104I CTCTTC 2 cut(s) 129, 285
EarI CTCTTC 2 cut(s) 129, 285
Eco130I CCWWGG 1 cut(s) 737
EcoRII CCWGG 1 cut(s) 531
EcoT14I CCWWGG 1 cut(s) 737
ErhI CCWWGG 1 cut(s) 737
FaeI CATG 2 cut(s) 403, 602
FaqI GGGAC 1 cut(s) 611
FatI CATG 2 cut(s) 399, 598
FblI GTMKAC 1 cut(s) 603
Fnu4HI GCNGC 4 cut(s) 146, 756, 858, 861
Fsp4HI GCNGC 4 cut(s) 146, 756, 858, 861
FspBI CTAG 2 cut(s) 75, 695
GluI GCNGC 4 cut(s) 146, 756, 858, 861
GsaI CCCAGC 1 cut(s) 41
GsuI CTGGAG 1 cut(s) 704
HaeIII GGCC 3 cut(s) 95, 108, 398
HapII CCGG 1 cut(s) 640
HgaI GACGC 1 cut(s) 317
Hin1II CATG 2 cut(s) 403, 602
HpaII CCGG 1 cut(s) 640
HphI GGTGA 4 cut(s) 130, 254, 266, 368
Hpy166II GTNNAC 2 cut(s) 225, 604
Hpy188I TCNGA 2 cut(s) 202, 633
Hpy188III TCNNGA 4 cut(s) 677, 683, 695, 911
Hpy8I GTNNAC 2 cut(s) 225, 604
HpyAV CCTTC 2 cut(s) 414, 446
HpyCH4III ACNGT 4 cut(s) 171, 330, 413, 525
HpyCH4IV ACGT 3 cut(s) 141, 152, 204
HpyCH4V TGCA 5 cut(s) 131, 148, 368, 403, 898
HpyF3I CTNAG 4 cut(s) 114, 393, 630, 654
HpySE526I ACGT 3 cut(s) 141, 152, 204
Hsp92II CATG 2 cut(s) 403, 602
Kzo9I GATC 5 cut(s) 362, 679, 774, 802, 903
LguI GCTCTTC 1 cut(s) 285
LmnI GCTCC 1 cut(s) 32
Lsp1109I GCAGC 4 cut(s) 132, 767, 844, 847
MaeI CTAG 2 cut(s) 75, 695
MaeII ACGT 3 cut(s) 141, 152, 204
MaeIII GTNAC 3 cut(s) 254, 545, 747
MalI GATC 5 cut(s) 364, 681, 776, 804, 905
MboI GATC 5 cut(s) 362, 679, 774, 802, 903
MboII GAAGA 7 cut(s) 146, 148, 302, 431, 436, 658, 902
MfeI CAATTG 1 cut(s) 899
MflI RGATCY 3 cut(s) 362, 679, 774
MhlI GDGCHC 1 cut(s) 29
MluCI AATT 4 cut(s) 229, 660, 701, 899
MmeI TCCRAC 1 cut(s) 74
MnlI CCTC 9 cut(s) 40, 57, 66, 107, 130, 210, 453, 639, 679
MseI TTAA 3 cut(s) 375, 515, 582
MslI CAYNNNNRTG 1 cut(s) 789
MspI CCGG 1 cut(s) 640
MspR9I CCNGG 1 cut(s) 533
MunI CAATTG 1 cut(s) 899
MvaI CCWGG 1 cut(s) 533
MvnI CGCG 1 cut(s) 551
NdeII GATC 5 cut(s) 362, 679, 774, 802, 903
NlaIII CATG 2 cut(s) 403, 602
NlaIV GGNNCC 2 cut(s) 538, 776
NmeAIII GCCGAG 1 cut(s) 71
NmuCI GTSAC 1 cut(s) 254
NspI RCATGY 1 cut(s) 602
PciSI GCTCTTC 1 cut(s) 285
PflMI CCANNNNNTGG 1 cut(s) 345
PkrI GCNGC 4 cut(s) 147, 757, 859, 862
PshBI ATTAAT 1 cut(s) 375
PsiI TTATAA 1 cut(s) 852
Psp6I CCWGG 1 cut(s) 531
PspFI CCCAGC 1 cut(s) 37
PspGI CCWGG 1 cut(s) 531
PspN4I GGNNCC 2 cut(s) 538, 776
PspPI GGNCC 2 cut(s) 94, 107
PsrI GAACNNNNNNTAC 2 cut(s) 484, 516
PstI CTGCAG 1 cut(s) 150
PstNI CAGNNNCTG 1 cut(s) 682
PsuI RGATCY 3 cut(s) 362, 679, 774
RsaI GTAC 3 cut(s) 502, 578, 593
RsaNI GTAC 3 cut(s) 501, 577, 592
RseI CAYNNNNRTG 1 cut(s) 789
SapI GCTCTTC 1 cut(s) 285
SaqAI TTAA 3 cut(s) 375, 515, 582
SatI GCNGC 4 cut(s) 146, 756, 858, 861
Sau3AI GATC 5 cut(s) 362, 679, 774, 802, 903
Sau96I GGNCC 2 cut(s) 94, 107
ScrFI CCNGG 1 cut(s) 533
SduI GDGCHC 1 cut(s) 29
SfcI CTRYAG 1 cut(s) 146
SmiMI CAYNNNNRTG 1 cut(s) 789
Sse9I AATT 4 cut(s) 229, 660, 701, 899
SspMI CTAG 2 cut(s) 75, 695
StyD4I CCNGG 1 cut(s) 531
StyI CCWWGG 1 cut(s) 737
TaaI ACNGT 4 cut(s) 171, 330, 413, 525
TaiI ACGT 3 cut(s) 144, 155, 207
TasI AATT 4 cut(s) 229, 660, 701, 899
TatI WGTACW 1 cut(s) 500
Tru1I TTAA 3 cut(s) 375, 515, 582
Tru9I TTAA 3 cut(s) 375, 515, 582
TscAI CASTG 3 cut(s) 418, 528, 805
TseFI GTSAC 1 cut(s) 254
TseI GCWGC 4 cut(s) 145, 755, 857, 860
Tsp45I GTSAC 1 cut(s) 254
TspDTI ATGAA 5 cut(s) 175, 357, 462, 720, 903
TspRI CASTG 3 cut(s) 418, 528, 805
Van91I CCANNNNNTGG 1 cut(s) 345
VspI ATTAAT 1 cut(s) 375
XapI RAATTY 1 cut(s) 229
XbaI TCTAGA 1 cut(s) 694
XceI RCATGY 1 cut(s) 602
XmiI GTMKAC 1 cut(s) 603
XspI CTAG 2 cut(s) 75, 695
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.