AT5G22820

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0000928
Physical Location & Seq
Reverse (-)
7622962 .. 7627037
4076 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G22820.1

Sequence Viewer

Length: 1473 bp
ATGGAAGACGATGAAGCGACGAAACAGCGAGGAATACTTGAAGAAGAAGAAGAAAATTTTACTGGAAGAAGAGAAGAAAGTGAAAACGAGTTTCCCTCACATCATCCTCCTCCTCCGCCTGATGAGTTGTTTGACATTTCGACTACAGTTGATCCTAGCTATCTTATTTCACTGATAAGGAAACTCCTTCCAATTGATTCGGGAAGTGACGAACGACATAATGATCATATGAATACGGATAATGTGGTTCAAGGTGTAGTAGCTATATCTGGAAATGGGGTTGTTGAGACTTCCAATGGTGATCCAGAGAGCATGGACATTGGAGATAATCATGATGAATCCACTTATGAGGTGGGGGAGACGGTTAGTTCATGTCCAGCGCCGGGAATGCAAGACGGGTCATCGGCATGGGAAGATCATGGTTGTGTTTTGTGGGATCTTGCCGCCAGTAGAACTCATGCTGAGCTAATGGTACAGAACCTAATTTTAGAAGTGCTTCACGCAAACCTTATGGTTTCAAAATCTCCCCGTATTCGAGAAATCTGTCTCGGGATTATTAGGAACCTTGCTTGCCATGAAGGTCTGTTGAAACATATAGAGTCTACAGCCGGAATCGTCAATACACTTGTGGGGCAGTTGTTTCTTGATGATACCCAATGCCTAAGTGAAGTTTGCAGGATACTCACCACAGGACTCTCTGGCGCTGGGTGTACTTCCTGGGCCCATTGTTTGGAGTCTGATGATATACTTCGCCATATTTTGTGGATTGCGGAAAATACCTTGAATCCTCATCTTATTGAAAAGAGTGTAGGGCTATTGTTAGGTATCATAGAAGGTCAACCTGAGGTTGAGCAGCTATTGATTCCTCCACTAATGAATCTGGGTTTGACGAGTCTCTTGATCAATCTTCTTTCATTTGAAATGAGCAAGTTGACTAAAGAAAGAATTCCAGAAAGGTACCCCGTCCTTGAAATTATTCTCCGAGCTATTGAAGCCCTTTCTGCTTCAGATAGCTATTCTAAAGAGATTTGCTCTAGCAAAGAACTTTTTCAATTAGTCTGTGATCTTATGAAACTTCAGGACAAAGCCGAGGTTGCAACATCTTGTGTTACTACTGGAGTTTTAATCGCAAATATGCTGTCTGAAAGAGTCGATTTCATTCCAGAAGTATTGGAAGATTTTTCTTTCTTGGAAGGTTTATTCAGTACTTTACCATTTGCTTCGGATGATGTAGAAGCAAGAAGAGCCCTTTGGAATGTTATTGCGAGATTACTTGCCAGAGTTAATGAATCCGAGATAAACACATTCCTTCTGAGTCAGTACATATTGGTTTTGTTAAGCAACGCAGATATTATTGAAGATGATTTTCTTGATACCCAACTAGAAGATTCAAACGAGTCCCGAAACTCATTCCCTTCCCAGATAAAGTCAAGCGCACGAACAATTGCTGTATCCACTTTTATATTCCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

490

Amino Acids

54.29

Weight (kDa)

4.41

Isoelectric Point (pI)

49.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 957
AccB1I GGYRCC 1 cut(s) 957
AccB7I CCANNNNNTGG 1 cut(s) 730
AccI GTMKAC 1 cut(s) 602
AciI CCGC 3 cut(s) 116, 444, 770
AclWI GGATC 3 cut(s) 146, 296, 444
AcsI RAATTY 2 cut(s) 55, 945
AcuI CTGAAG 2 cut(s) 990, 1061
AfaI GTAC 5 cut(s) 474, 712, 959, 1207, 1322
AfiI CCNNNNNNNGG 2 cut(s) 383, 730
AjnI CCWGG 1 cut(s) 716
AjuI GAANNNNNNNTTGG 2 cut(s) 1234, 1266
AluBI AGCT 6 cut(s) 159, 263, 466, 856, 986, 1014
AluI AGCT 6 cut(s) 159, 263, 466, 856, 986, 1014
Alw26I GTCTC 4 cut(s) 281, 353, 551, 899
AlwI GGATC 3 cut(s) 146, 296, 444
Ama87I CYCGRG 1 cut(s) 548
AoxI GGCC 1 cut(s) 720
ApaI GGGCCC 1 cut(s) 724
ApeKI GCWGC 1 cut(s) 853
ApoI RAATTY 2 cut(s) 55, 945
ArsI GACNNNNNNTTYG 2 cut(s) 1124, 1156
Asp700I GAANNNNTTC 4 cut(s) 495, 945, 975, 1047
Asp718I GGTACC 1 cut(s) 957
AspLEI GCGC 3 cut(s) 382, 704, 1436
AspS9I GGNCC 2 cut(s) 720, 721
AsuC2I CCSGG 1 cut(s) 384
AsuHPI GGTGA 2 cut(s) 311, 676
AvaI CYCGRG 1 cut(s) 548
AxyI CCTNAGG 1 cut(s) 843
BaeGI GKGCMC 1 cut(s) 724
BanI GGYRCC 1 cut(s) 957
BanII GRGCYC 2 cut(s) 724, 1249
BbsI GAAGAC 1 cut(s) 12
BbvI GCAGC 1 cut(s) 865
BciT130I CCWGG 1 cut(s) 718
BciVI GTATCC 2 cut(s) 672, 1462
BclI TGATCA 2 cut(s) 223, 900
BcnI CCSGG 1 cut(s) 384
BcoDI GTCTC 4 cut(s) 281, 353, 551, 899
BfaI CTAG 3 cut(s) 156, 1035, 1382
BfmI CTRYAG 2 cut(s) 144, 603
BfoI RGCGCY 2 cut(s) 383, 705
BfuI GTATCC 2 cut(s) 672, 1462
BisI GCNGC 2 cut(s) 444, 854
BlpI GCTNAGC 1 cut(s) 462
BlsI GCNGC 2 cut(s) 445, 855
BmcAI AGTACT 1 cut(s) 1207
Bme1390I CCNGG 2 cut(s) 384, 718
BmeT110I CYCGRG 1 cut(s) 548
BmgT120I GGNCC 2 cut(s) 720, 721
BmiI GGNNCC 3 cut(s) 563, 722, 959
BmrFI CCNGG 2 cut(s) 384, 718
BpiI GAAGAC 1 cut(s) 12
BplI GAGNNNNNCTC 4 cut(s) 80, 112, 1016, 1048
BpmI CTGGAG 1 cut(s) 1137
Bpu1102I GCTNAGC 1 cut(s) 462
BpuMI CCSGG 1 cut(s) 384
BsaJI CCNNGG 2 cut(s) 717, 1089
Bsc4I CCNNNNNNNGG 2 cut(s) 383, 730
Bse1I ACTGG 3 cut(s) 67, 447, 1120
Bse21I CCTNAGG 1 cut(s) 843
BseBI CCWGG 1 cut(s) 718
BseDI CCNNGG 2 cut(s) 717, 1089
BseGI GGATG 2 cut(s) 103, 1231
BseLI CCNNNNNNNGG 2 cut(s) 383, 730
BseMII CTCAG 3 cut(s) 453, 834, 1304
BseNI ACTGG 3 cut(s) 67, 447, 1120
BseRI GAGGAG 2 cut(s) 99, 102
BseSI GKGCMC 1 cut(s) 724
BseXI GCAGC 1 cut(s) 865
BseYI CCCAGC 1 cut(s) 704
BshFI GGCC 1 cut(s) 722
BshNI GGYRCC 1 cut(s) 957
BsiHKCI CYCGRG 1 cut(s) 548
BsiSI CCGG 2 cut(s) 383, 609
BslFI GGGAC 1 cut(s) 1384
BslI CCNNNNNNNGG 2 cut(s) 383, 730
BsmAI GTCTC 4 cut(s) 281, 353, 551, 899
BsmBI CGTCTC 1 cut(s) 353
BsmFI GGGAC 1 cut(s) 1384
BsmI GAATGC 1 cut(s) 393
BsnI GGCC 1 cut(s) 722
BsoBI CYCGRG 1 cut(s) 548
Bsp120I GGGCCC 1 cut(s) 720
Bsp1286I GDGCHC 2 cut(s) 724, 1249
Bsp143I GATC 7 cut(s) 151, 223, 301, 415, 436, 900, 1063
Bsp1720I GCTNAGC 1 cut(s) 462
BspACI CCGC 3 cut(s) 116, 444, 770
BspANI GGCC 1 cut(s) 722
BspCNI CTCAG 3 cut(s) 454, 835, 1305
BspHI TCATGA 1 cut(s) 331
BspLI GGNNCC 3 cut(s) 563, 722, 959
BspPI GGATC 3 cut(s) 146, 296, 444
BspQI GCTCTTC 1 cut(s) 1237
BspT107I GGYRCC 1 cut(s) 957
BsrI ACTGG 3 cut(s) 67, 447, 1120
BssECI CCNNGG 2 cut(s) 717, 1089
BssMI GATC 7 cut(s) 151, 223, 301, 415, 436, 900, 1063
Bst2UI CCWGG 1 cut(s) 718
Bst4CI ACNGT 2 cut(s) 148, 364
Bst6I CTCTTC 2 cut(s) 64, 1237
BstC8I GCNNGC 1 cut(s) 571
BstDEI CTNAG 4 cut(s) 462, 662, 843, 1313
BstF5I GGATG 2 cut(s) 103, 1231
BstH2I RGCGCY 2 cut(s) 383, 705
BstHHI GCGC 3 cut(s) 382, 704, 1436
BstKTI GATC 7 cut(s) 154, 226, 304, 418, 439, 903, 1066
BstMAI GTCTC 4 cut(s) 281, 353, 551, 899
BstMBI GATC 7 cut(s) 151, 223, 301, 415, 436, 900, 1063
BstMWI GCNNNNNNNGC 5 cut(s) 388, 992, 1001, 1094, 1244
BstNI CCWGG 1 cut(s) 718
BstSCI CCNGG 2 cut(s) 382, 716
BstSFI CTRYAG 2 cut(s) 144, 603
BstSLI GKGCMC 1 cut(s) 724
BstV1I GCAGC 1 cut(s) 865
BstV2I GAAGAC 1 cut(s) 12
BstX2I RGATCY 1 cut(s) 436
BstYI RGATCY 1 cut(s) 436
Bsu36I CCTNAGG 1 cut(s) 843
BsuI GTATCC 2 cut(s) 672, 1462
BsuRI GGCC 1 cut(s) 722
BtsCI GGATG 2 cut(s) 103, 1231
BtsIMutI CAGTG 1 cut(s) 170
Cac8I GCNNGC 1 cut(s) 571
CciI TCATGA 1 cut(s) 331
CfoI GCGC 3 cut(s) 382, 704, 1436
Cfr13I GGNCC 2 cut(s) 720, 721
Csp6I GTAC 5 cut(s) 473, 711, 958, 1206, 1321
CviAII CATG 7 cut(s) 313, 332, 372, 408, 419, 458, 575
CviQI GTAC 5 cut(s) 473, 711, 958, 1206, 1321
DdeI CTNAG 4 cut(s) 462, 662, 843, 1313
DpnI GATC 7 cut(s) 153, 225, 303, 417, 438, 902, 1065
DpnII GATC 7 cut(s) 151, 223, 301, 415, 436, 900, 1063
Eam1104I CTCTTC 2 cut(s) 64, 1237
EarI CTCTTC 2 cut(s) 64, 1237
EciI GGCGGA 1 cut(s) 105
Eco24I GRGCYC 2 cut(s) 724, 1249
Eco57I CTGAAG 2 cut(s) 990, 1061
Eco81I CCTNAGG 1 cut(s) 843
Eco88I CYCGRG 1 cut(s) 548
EcoRI GAATTC 1 cut(s) 945
EcoRII CCWGG 1 cut(s) 716
EcoT38I GRGCYC 2 cut(s) 724, 1249
Esp3I CGTCTC 1 cut(s) 353
FaeI CATG 7 cut(s) 316, 335, 375, 411, 422, 461, 578
FaqI GGGAC 1 cut(s) 1384
FatI CATG 7 cut(s) 312, 331, 371, 407, 418, 457, 574
FauNDI CATATG 1 cut(s) 228
FbaI TGATCA 2 cut(s) 223, 900
FblI GTMKAC 1 cut(s) 602
Fnu4HI GCNGC 2 cut(s) 444, 854
FokI GGATG 2 cut(s) 90, 1238
FriOI GRGCYC 2 cut(s) 724, 1249
Fsp4HI GCNGC 2 cut(s) 444, 854
FspBI CTAG 3 cut(s) 156, 1035, 1382
GlaI GCGC 3 cut(s) 381, 703, 1435
GluI GCNGC 2 cut(s) 444, 854
GsaI CCCAGC 1 cut(s) 708
GsuI CTGGAG 1 cut(s) 1137
HaeII RGCGCY 2 cut(s) 383, 705
HaeIII GGCC 1 cut(s) 722
HapII CCGG 2 cut(s) 383, 609
HhaI GCGC 3 cut(s) 382, 704, 1436
Hin1II CATG 7 cut(s) 316, 335, 375, 411, 422, 461, 578
Hin6I GCGC 3 cut(s) 380, 702, 1434
HinP1I GCGC 3 cut(s) 380, 702, 1434
HincII GTYRAC 2 cut(s) 839, 933
HindII GTYRAC 2 cut(s) 839, 933
HpaII CCGG 2 cut(s) 383, 609
HphI GGTGA 2 cut(s) 311, 676
Hpy166II GTNNAC 4 cut(s) 603, 711, 839, 933
Hpy188I TCNGA 7 cut(s) 739, 983, 1009, 1144, 1225, 1294, 1314
Hpy8I GTNNAC 4 cut(s) 603, 711, 839, 933
Hpy99I CGWCG 1 cut(s) 22
HpyAV CCTTC 6 cut(s) 197, 572, 827, 1187, 1319, 1425
HpyCH4III ACNGT 2 cut(s) 148, 364
HpyCH4V TGCA 3 cut(s) 391, 675, 1097
HpyF10VI GCNNNNNNNGC 5 cut(s) 388, 992, 1001, 1094, 1244
HpyF3I CTNAG 4 cut(s) 462, 662, 843, 1313
Hsp92II CATG 7 cut(s) 316, 335, 375, 411, 422, 461, 578
HspAI GCGC 3 cut(s) 380, 702, 1434
KpnI GGTACC 1 cut(s) 961
Ksp22I TGATCA 2 cut(s) 223, 900
Kzo9I GATC 7 cut(s) 151, 223, 301, 415, 436, 900, 1063
LguI GCTCTTC 1 cut(s) 1237
Lsp1109I GCAGC 1 cut(s) 865
MaeI CTAG 3 cut(s) 156, 1035, 1382
MaeIII GTNAC 2 cut(s) 206, 1108
MalI GATC 7 cut(s) 153, 225, 303, 417, 438, 902, 1065
MboI GATC 7 cut(s) 151, 223, 301, 415, 436, 900, 1063
MfeI CAATTG 2 cut(s) 192, 1443
MflI RGATCY 1 cut(s) 436
MhlI GDGCHC 2 cut(s) 724, 1249
MluCI AATT 7 cut(s) 55, 192, 483, 945, 972, 1052, 1443
MlyI GAGTC 7 cut(s) 608, 687, 743, 901, 1158, 1324, 1406
MroXI GAANNNNTTC 4 cut(s) 495, 945, 975, 1047
MseI TTAA 3 cut(s) 1124, 1284, 1337
MslI CAYNNNNRTG 2 cut(s) 406, 423
MspI CCGG 2 cut(s) 383, 609
MspR9I CCNGG 2 cut(s) 384, 718
MunI CAATTG 2 cut(s) 192, 1443
Mva1269I GAATGC 1 cut(s) 393
MvaI CCWGG 1 cut(s) 718
MwoI GCNNNNNNNGC 5 cut(s) 388, 992, 1001, 1094, 1244
NciI CCSGG 1 cut(s) 384
NdeI CATATG 1 cut(s) 228
NdeII GATC 7 cut(s) 151, 223, 301, 415, 436, 900, 1063
NlaIII CATG 7 cut(s) 316, 335, 375, 411, 422, 461, 578
NlaIV GGNNCC 3 cut(s) 563, 722, 959
NmeAIII GCCGAG 1 cut(s) 1114
NmuCI GTSAC 1 cut(s) 206
PagI TCATGA 1 cut(s) 331
PciSI GCTCTTC 1 cut(s) 1237
PctI GAATGC 1 cut(s) 393
PdmI GAANNNNTTC 4 cut(s) 495, 945, 975, 1047
PfeI GAWTC 8 cut(s) 197, 338, 612, 784, 862, 877, 1289, 1388
PflMI CCANNNNNTGG 1 cut(s) 730
PkrI GCNGC 2 cut(s) 445, 855
PleI GAGTC 7 cut(s) 607, 687, 742, 900, 1157, 1323, 1405
PpsI GAGTC 7 cut(s) 607, 687, 742, 900, 1157, 1323, 1405
Psp6I CCWGG 1 cut(s) 716
PspFI CCCAGC 1 cut(s) 704
PspGI CCWGG 1 cut(s) 716
PspN4I GGNNCC 3 cut(s) 563, 722, 959
PspOMI GGGCCC 1 cut(s) 720
PspPI GGNCC 2 cut(s) 720, 721
PsuI RGATCY 1 cut(s) 436
RsaI GTAC 5 cut(s) 474, 712, 959, 1207, 1322
RsaNI GTAC 5 cut(s) 473, 711, 958, 1206, 1321
RseI CAYNNNNRTG 2 cut(s) 406, 423
SapI GCTCTTC 1 cut(s) 1237
SaqAI TTAA 3 cut(s) 1124, 1284, 1337
SatI GCNGC 2 cut(s) 444, 854
Sau3AI GATC 7 cut(s) 151, 223, 301, 415, 436, 900, 1063
Sau96I GGNCC 2 cut(s) 720, 721
ScaI AGTACT 1 cut(s) 1207
SchI GAGTC 7 cut(s) 608, 687, 743, 901, 1158, 1324, 1406
ScrFI CCNGG 2 cut(s) 384, 718
SduI GDGCHC 2 cut(s) 724, 1249
SfcI CTRYAG 2 cut(s) 144, 603
SmiMI CAYNNNNRTG 2 cut(s) 406, 423
Sse9I AATT 7 cut(s) 55, 192, 483, 945, 972, 1052, 1443
SsiI CCGC 3 cut(s) 116, 444, 770
SspMI CTAG 3 cut(s) 156, 1035, 1382
StyD4I CCNGG 2 cut(s) 382, 716
TaaI ACNGT 2 cut(s) 148, 364
TaqI TCGA 3 cut(s) 140, 535, 1152
TasI AATT 7 cut(s) 55, 192, 483, 945, 972, 1052, 1443
TatI WGTACW 3 cut(s) 710, 1205, 1320
TauI GCSGC 1 cut(s) 446
TfiI GAWTC 8 cut(s) 197, 338, 612, 784, 862, 877, 1289, 1388
Tru1I TTAA 3 cut(s) 1124, 1284, 1337
Tru9I TTAA 3 cut(s) 1124, 1284, 1337
TscAI CASTG 1 cut(s) 177
TseFI GTSAC 1 cut(s) 206
TseI GCWGC 1 cut(s) 853
Tsp45I GTSAC 1 cut(s) 206
TspGWI ACGGA 1 cut(s) 251
TspRI CASTG 1 cut(s) 177
Van91I CCANNNNNTGG 1 cut(s) 730
XapI RAATTY 2 cut(s) 55, 945
XcmI CCANNNNNNNNNTGG 1 cut(s) 349
XmiI GTMKAC 1 cut(s) 602
XmnI GAANNNNTTC 4 cut(s) 495, 945, 975, 1047
XspI CTAG 3 cut(s) 156, 1035, 1382
ZrmI AGTACT 1 cut(s) 1207
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.