Rh5CG400900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0000928
Physical Location & Seq
Reverse (-)
53362487 .. 53369415
6929 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG400900.1

Sequence Viewer

Length: 1596 bp
ATGGCGCCGGACTCAAAATCAGCACCCCTACAACACCAGGAAGAAGAAGAACAACAACCGGAAGACCTCGACACACTTTCACACAACCCTTCTGCTCCGCCTGATGAGTTATTCGACATCTCAACTACAGTAGATCCGAGTTATGTGATCTCTCTGATACGGAAACTTCTACCAGCTAATGGCAGTAATAATCCCAACTATCGCGGTGATGTTTCCTGTGGCCCCGTGCAAGGATTAAATGTTGATGACATGGAAAAAAGTGCTCTAACTCGCTCTGGTGTTCCACCCCCATCAACGGACACATCTGAGAGCATGGAAATTAATGACGATTTTAATGAAAATGCTACCCATGAAGGAGAAAGTGAGGCGGAACAGCCTCGTCATAGTGTGCCGGTGGGAGAAGAGGCTTGGGAAGAGTATGGTTGCATATTATGGGATCTTTCTGCAAGTAAAACTCATGCAGAACTCATGGTGCAGAACCTTGTACTTGAAGTGCTGTTAGCAAACCTTATGGTTTCACAATCCGTTCGTACTACGGAAATTGGTCTCGGAATCATTGGAAACCTTGCCTGTCATGAAGTTCCCATGAAACATATAGTCTCTACTAATGGATTAATTGAATTCATTGTGGATCAGATGTTTCTGGATGATGCTCAATGCTTATGTGAAGTCTGCAGACTGTTAACTGTGGGTCTTCAAAGTAGTGAAGGCGTTACTTGGGCTGAGGCATTGCAGTCGGAACATTATCTGACCCACATTTTATGGATTGCAGAAAATAGCTTGAACACACAGCTTATAGAAAAGAGTGCTGAACTATTGTTAGCGATCATCGAAAGTTCGCAGGATGTTATGCATATTCTCTTACCACCATTGATGAAGCTGGGCTTAGCAAGTCTATTAATAAATCTCTTGGATATTGAAACAAGCAAATTAATGAGTGAAAGAGCTCCTGAAAGGTACCCAATTCTTGATGTAGTTCTTCGTGCCATTGAAGCTCTTTCTGTTATTGATGATCATTCCCAGGACATCTGTTTAGACAAGGATCTCTTTCAGCTGGTTTGTGCCTTGCTCAAGTTCCCTGATAAAGTTGAGGTTGCAAACTCTTGTGTTACAGCTGTAGTTCTAGTTGCAAATATTCTTTCAGACGTACCTACTCTAGCATCAGAGTTATCACATGATATGCTTTTCTTACAGGGTCTGCTTGATGTGTTTCCCTTTACTTCGGATGACTCAGAAGCAAGGAGTGCACTGTGGAACATCATTGCAAGGTTACTGGTTCGACTTCCAGAAAATGAAATGAGCTCATCAACTATCCAGCAGTATGTTTTGGTTCTGGTTAGCAAATCTGATGTGATTGAAGATGATCTTCTTGACTGCCAGTTAGGTGGCTTAGGATTAAAGGCTAGAACTACAGCTGTATCCTGTTCTGTGAATTTTTTGTTGAACTTGCAGTTAAAATGCAATAAAGTTGGCAGTTTGAATCTTATTCCAGCAATATCTAAGGTCAATACTGCTTCACTTCTCAAGTCCTGTCTTGCAATATATTTGGTTTTCCTTTATTTTTGCTACAGCTCAGAAGGATCATCAGTATTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

531

Amino Acids

58.25

Weight (kDa)

4.36

Isoelectric Point (pI)

57.22

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 957
AccB1I GGYRCC 2 cut(s) 4, 957
AccB7I CCANNNNNTGG 1 cut(s) 179
AccII CGCG 1 cut(s) 204
AciI CCGC 3 cut(s) 98, 204, 368
AclWI GGATC 5 cut(s) 128, 444, 639, 1050, 1588
AcsI RAATTY 2 cut(s) 620, 1432
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 4 cut(s) 486, 532, 959, 1149
AfiI CCNNNNNNNGG 3 cut(s) 179, 230, 295
AgsI TTSAA 9 cut(s) 491, 620, 698, 784, 920, 992, 1358, 1444, 1480
AjnI CCWGG 2 cut(s) 36, 1020
Alw21I GWGCWC 4 cut(s) 265, 949, 1249, 1304
Alw26I GTCTC 2 cut(s) 551, 604
Alw44I GTGCAC 1 cut(s) 1245
AlwI GGATC 5 cut(s) 128, 444, 639, 1050, 1588
AlwNI CAGNNNCTG 1 cut(s) 1198
AoxI GGCC 1 cut(s) 220
ApaLI GTGCAC 1 cut(s) 1245
ApoI RAATTY 2 cut(s) 620, 1432
AseI ATTAAT 4 cut(s) 321, 614, 899, 932
Asp718I GGTACC 1 cut(s) 957
AspLEI GCGC 1 cut(s) 7
AspS9I GGNCC 1 cut(s) 221
AsuHPI GGTGA 1 cut(s) 218
BaeGI GKGCMC 1 cut(s) 1249
BaeI ACNNNNGTAYC 2 cut(s) 1401, 1434
BanI GGYRCC 2 cut(s) 4, 957
BanII GRGCYC 2 cut(s) 949, 1304
BbsI GAAGAC 2 cut(s) 69, 686
Bbv12I GWGCWC 4 cut(s) 265, 949, 1249, 1304
BbvCI CCTCAGC 1 cut(s) 723
BccI CCATC 1 cut(s) 298
BcgI CGANNNNNNTGC 2 cut(s) 717, 751
BciT130I CCWGG 2 cut(s) 38, 1022
BciVI GTATCC 1 cut(s) 1429
BclI TGATCA 1 cut(s) 1012
BcoDI GTCTC 2 cut(s) 551, 604
BfaI CTAG 3 cut(s) 1124, 1157, 1404
BfmI CTRYAG 5 cut(s) 126, 673, 1116, 1410, 1567
BfoI RGCGCY 1 cut(s) 8
BfuI GTATCC 1 cut(s) 1429
BlpI GCTNAGC 1 cut(s) 886
Bme1390I CCNGG 2 cut(s) 38, 1022
BmgT120I GGNCC 1 cut(s) 221
BmiI GGNNCC 3 cut(s) 6, 223, 959
BmrFI CCNGG 2 cut(s) 38, 1022
BmsI GCATC 2 cut(s) 640, 1169
BpiI GAAGAC 2 cut(s) 69, 686
Bpu10I CCTNAGC 2 cut(s) 723, 1390
Bpu1102I GCTNAGC 1 cut(s) 886
BpuEI CTTGAG 2 cut(s) 1055, 1508
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 551
BsaJI CCNNGG 1 cut(s) 1020
BsaWI WCCGGW 1 cut(s) 58
Bsc4I CCNNNNNNNGG 3 cut(s) 179, 230, 295
Bse118I RCCGGY 1 cut(s) 391
Bse1I ACTGG 2 cut(s) 1278, 1378
Bse3DI GCAATG 2 cut(s) 728, 1260
BseBI CCWGG 2 cut(s) 38, 1022
BseDI CCNNGG 1 cut(s) 1020
BseGI GGATG 3 cut(s) 652, 850, 1231
BseLI CCNNNNNNNGG 3 cut(s) 179, 230, 295
BseMI GCAATG 2 cut(s) 728, 1260
BseMII CTCAG 4 cut(s) 297, 714, 1245, 1587
BseNI ACTGG 2 cut(s) 1278, 1378
BseSI GKGCMC 1 cut(s) 1249
BseYI CCCAGC 1 cut(s) 880
BsgI GTGCAG 1 cut(s) 494
Bsh1236I CGCG 1 cut(s) 204
BshFI GGCC 1 cut(s) 222
BshNI GGYRCC 2 cut(s) 4, 957
BsiHKAI GWGCWC 4 cut(s) 265, 949, 1249, 1304
BsiSI CCGG 3 cut(s) 8, 59, 392
BslI CCNNNNNNNGG 3 cut(s) 179, 230, 295
BsmAI GTCTC 2 cut(s) 551, 604
BsnI GGCC 1 cut(s) 222
Bso31I GGTCTC 1 cut(s) 551
Bsp1286I GDGCHC 4 cut(s) 265, 949, 1249, 1304
Bsp143I GATC 9 cut(s) 133, 147, 436, 631, 825, 1012, 1042, 1363, 1580
Bsp1720I GCTNAGC 1 cut(s) 886
BspACI CCGC 3 cut(s) 98, 204, 368
BspANI GGCC 1 cut(s) 222
BspCNI CTCAG 4 cut(s) 298, 715, 1244, 1586
BspFNI CGCG 1 cut(s) 204
BspHI TCATGA 1 cut(s) 574
BspLI GGNNCC 3 cut(s) 6, 223, 959
BspMAI CTGCAG 1 cut(s) 677
BspPI GGATC 5 cut(s) 128, 444, 639, 1050, 1588
BspT107I GGYRCC 2 cut(s) 4, 957
BspTNI GGTCTC 1 cut(s) 551
BsrDI GCAATG 2 cut(s) 728, 1260
BsrFI RCCGGY 1 cut(s) 391
BsrI ACTGG 2 cut(s) 1278, 1378
BssAI RCCGGY 1 cut(s) 391
BssECI CCNNGG 1 cut(s) 1020
BssMI GATC 9 cut(s) 133, 147, 436, 631, 825, 1012, 1042, 1363, 1580
BssNI GRCGYC 1 cut(s) 5
Bst2UI CCWGG 2 cut(s) 38, 1022
Bst4CI ACNGT 4 cut(s) 130, 681, 688, 1251
Bst6I CTCTTC 2 cut(s) 396, 408
BstACI GRCGYC 1 cut(s) 5
BstAPI GCANNNNNTGC 1 cut(s) 1244
BstDEI CTNAG 7 cut(s) 306, 723, 886, 1231, 1390, 1500, 1573
BstF5I GGATG 3 cut(s) 652, 850, 1231
BstFNI CGCG 1 cut(s) 204
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 1 cut(s) 7
BstKTI GATC 9 cut(s) 136, 150, 439, 634, 828, 1015, 1045, 1366, 1583
BstMAI GTCTC 2 cut(s) 551, 604
BstMBI GATC 9 cut(s) 133, 147, 436, 631, 825, 1012, 1042, 1363, 1580
BstMWI GCNNNNNNNGC 2 cut(s) 992, 1244
BstNI CCWGG 2 cut(s) 38, 1022
BstSCI CCNGG 2 cut(s) 36, 1020
BstSFI CTRYAG 5 cut(s) 126, 673, 1116, 1410, 1567
BstSLI GKGCMC 1 cut(s) 1249
BstUI CGCG 1 cut(s) 204
BstV2I GAAGAC 2 cut(s) 69, 686
BstX2I RGATCY 3 cut(s) 133, 436, 1042
BstXI CCANNNNNNTGG 1 cut(s) 1385
BstYI RGATCY 3 cut(s) 133, 436, 1042
BsuI GTATCC 1 cut(s) 1429
BsuRI GGCC 1 cut(s) 222
BtsCI GGATG 3 cut(s) 652, 850, 1231
BtsIMutI CAGTG 1 cut(s) 1247
CaiI CAGNNNCTG 1 cut(s) 1198
CciI TCATGA 1 cut(s) 574
CfoI GCGC 1 cut(s) 7
Cfr10I RCCGGY 1 cut(s) 391
Cfr13I GGNCC 1 cut(s) 221
Csp6I GTAC 4 cut(s) 485, 531, 958, 1148
CviAII CATG 8 cut(s) 250, 313, 350, 458, 469, 575, 586, 1175
CviQI GTAC 4 cut(s) 485, 531, 958, 1148
DdeI CTNAG 7 cut(s) 306, 723, 886, 1231, 1390, 1500, 1573
DinI GGCGCC 1 cut(s) 6
DpnI GATC 9 cut(s) 135, 149, 438, 633, 827, 1014, 1044, 1365, 1582
DpnII GATC 9 cut(s) 133, 147, 436, 631, 825, 1012, 1042, 1363, 1580
Eam1104I CTCTTC 2 cut(s) 396, 408
EarI CTCTTC 2 cut(s) 396, 408
EciI GGCGGA 2 cut(s) 87, 383
Ecl136II GAGCTC 2 cut(s) 947, 1302
Eco24I GRGCYC 2 cut(s) 949, 1304
Eco31I GGTCTC 1 cut(s) 551
Eco53kI GAGCTC 2 cut(s) 947, 1302
EcoICRI GAGCTC 2 cut(s) 947, 1302
EcoRI GAATTC 1 cut(s) 620
EcoRII CCWGG 2 cut(s) 36, 1020
EcoT22I ATGCAT 1 cut(s) 855
EcoT38I GRGCYC 2 cut(s) 949, 1304
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
FaeI CATG 8 cut(s) 253, 316, 353, 461, 472, 578, 589, 1178
FalI AAGNNNNNCTT 6 cut(s) 869, 901, 1031, 1063, 1350, 1382
FatI CATG 8 cut(s) 249, 312, 349, 457, 468, 574, 585, 1174
FbaI TGATCA 1 cut(s) 1012
FokI GGATG 3 cut(s) 659, 857, 1238
FriOI GRGCYC 2 cut(s) 949, 1304
FspBI CTAG 3 cut(s) 1124, 1157, 1404
GlaI GCGC 1 cut(s) 6
GsaI CCCAGC 1 cut(s) 884
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 1 cut(s) 222
HapII CCGG 3 cut(s) 8, 59, 392
HhaI GCGC 1 cut(s) 7
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 8 cut(s) 253, 316, 353, 461, 472, 578, 589, 1178
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HincII GTYRAC 1 cut(s) 684
HindII GTYRAC 1 cut(s) 684
HinfI GANTC 4 cut(s) 11, 552, 1229, 1480
HpaI GTTAAC 1 cut(s) 684
HpaII CCGG 3 cut(s) 8, 59, 392
HphI GGTGA 1 cut(s) 218
Hpy166II GTNNAC 2 cut(s) 684, 1247
Hpy188III TCNNGA 6 cut(s) 575, 644, 950, 968, 1286, 1370
Hpy8I GTNNAC 2 cut(s) 684, 1247
HpyAV CCTTC 4 cut(s) 99, 347, 701, 1571
HpyCH4III ACNGT 4 cut(s) 130, 681, 688, 1251
HpyCH4IV ACGT 1 cut(s) 1146
HpyF10VI GCNNNNNNNGC 2 cut(s) 992, 1244
HpyF3I CTNAG 7 cut(s) 306, 723, 886, 1231, 1390, 1500, 1573
HpySE526I ACGT 1 cut(s) 1146
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 8 cut(s) 253, 316, 353, 461, 472, 578, 589, 1178
HspAI GCGC 1 cut(s) 5
KasI GGCGCC 1 cut(s) 4
KpnI GGTACC 1 cut(s) 961
Ksp22I TGATCA 1 cut(s) 1012
KspAI GTTAAC 1 cut(s) 684
Kzo9I GATC 9 cut(s) 133, 147, 436, 631, 825, 1012, 1042, 1363, 1580
LmnI GCTCC 2 cut(s) 100, 952
LweI GCATC 2 cut(s) 640, 1169
MaeI CTAG 3 cut(s) 1124, 1157, 1404
MaeII ACGT 1 cut(s) 1146
MaeIII GTNAC 3 cut(s) 712, 1108, 1269
MalI GATC 9 cut(s) 135, 149, 438, 633, 827, 1014, 1044, 1365, 1582
MboI GATC 9 cut(s) 133, 147, 436, 631, 825, 1012, 1042, 1363, 1580
MflI RGATCY 3 cut(s) 133, 436, 1042
MhlI GDGCHC 4 cut(s) 265, 949, 1249, 1304
MluCI AATT 7 cut(s) 318, 540, 615, 620, 929, 963, 1432
Mly113I GGCGCC 1 cut(s) 5
MlyI GAGTC 2 cut(s) 5, 1223
MmeI TCCRAC 1 cut(s) 717
MnlI CCTC 6 cut(s) 77, 358, 387, 397, 718, 1084
Mph1103I ATGCAT 1 cut(s) 855
MspA1I CMGCKG 3 cut(s) 1054, 1115, 1415
MspI CCGG 3 cut(s) 8, 59, 392
MspR9I CCNGG 2 cut(s) 38, 1022
MvaI CCWGG 2 cut(s) 38, 1022
MvnI CGCG 1 cut(s) 204
MwoI GCNNNNNNNGC 2 cut(s) 992, 1244
NarI GGCGCC 1 cut(s) 5
NdeII GATC 9 cut(s) 133, 147, 436, 631, 825, 1012, 1042, 1363, 1580
NlaIII CATG 8 cut(s) 253, 316, 353, 461, 472, 578, 589, 1178
NlaIV GGNNCC 3 cut(s) 6, 223, 959
NsiI ATGCAT 1 cut(s) 855
PagI TCATGA 1 cut(s) 574
PfeI GAWTC 2 cut(s) 552, 1480
PflMI CCANNNNNTGG 1 cut(s) 179
PleI GAGTC 2 cut(s) 5, 1223
PluTI GGCGCC 1 cut(s) 8
PpsI GAGTC 2 cut(s) 5, 1223
PshBI ATTAAT 4 cut(s) 321, 614, 899, 932
Psp124BI GAGCTC 2 cut(s) 949, 1304
Psp6I CCWGG 2 cut(s) 36, 1020
PspFI CCCAGC 1 cut(s) 880
PspGI CCWGG 2 cut(s) 36, 1020
PspN4I GGNNCC 3 cut(s) 6, 223, 959
PspPI GGNCC 1 cut(s) 221
PstI CTGCAG 1 cut(s) 677
PstNI CAGNNNCTG 1 cut(s) 1198
PsuI RGATCY 3 cut(s) 133, 436, 1042
PvuII CAGCTG 3 cut(s) 1054, 1115, 1415
RsaI GTAC 4 cut(s) 486, 532, 959, 1149
RsaNI GTAC 4 cut(s) 485, 531, 958, 1148
SacI GAGCTC 2 cut(s) 949, 1304
Sau3AI GATC 9 cut(s) 133, 147, 436, 631, 825, 1012, 1042, 1363, 1580
Sau96I GGNCC 1 cut(s) 221
SchI GAGTC 2 cut(s) 5, 1223
ScrFI CCNGG 2 cut(s) 38, 1022
SduI GDGCHC 4 cut(s) 265, 949, 1249, 1304
SfaNI GCATC 2 cut(s) 640, 1169
SfcI CTRYAG 5 cut(s) 126, 673, 1116, 1410, 1567
SfoI GGCGCC 1 cut(s) 6
SmlI CTYRAG 2 cut(s) 1070, 1523
SmoI CTYRAG 2 cut(s) 1070, 1523
Sse9I AATT 7 cut(s) 318, 540, 615, 620, 929, 963, 1432
SsiI CCGC 3 cut(s) 98, 204, 368
SspDI GGCGCC 1 cut(s) 4
SspI AATATT 1 cut(s) 1135
SspMI CTAG 3 cut(s) 1124, 1157, 1404
SstI GAGCTC 2 cut(s) 949, 1304
StyD4I CCNGG 2 cut(s) 36, 1020
TaaI ACNGT 4 cut(s) 130, 681, 688, 1251
TaiI ACGT 1 cut(s) 1149
TaqI TCGA 4 cut(s) 69, 114, 831, 1279
TasI AATT 7 cut(s) 318, 540, 615, 620, 929, 963, 1432
TatI WGTACW 1 cut(s) 484
TfiI GAWTC 2 cut(s) 552, 1480
TscAI CASTG 1 cut(s) 1254
TspDTI ATGAA 7 cut(s) 351, 366, 591, 602, 613, 890, 1308
TspGWI ACGGA 4 cut(s) 175, 311, 514, 551
TspRI CASTG 1 cut(s) 1254
Van91I CCANNNNNTGG 1 cut(s) 179
VneI GTGCAC 1 cut(s) 1245
VspI ATTAAT 4 cut(s) 321, 614, 899, 932
XapI RAATTY 2 cut(s) 620, 1432
XspI CTAG 3 cut(s) 1124, 1157, 1404
Zsp2I ATGCAT 1 cut(s) 855
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.