MD11G1153600.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0000928
Physical Location & Seq
Forward (+)
14733477 .. 14739692
6216 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1153600.v1.1.491

Sequence Viewer

Length: 1581 bp
ATGGCGCTGGACTCCAATTCAATACCTCTACAGGACCAAGAAGAACAAGAACCACAGTTACAAGACCACAACGCAACCGCACACCACCCTTCTGCTCCCCCTGATGAGCTGTTTGACATCTCAACAACAGTAGATCCTAGTTATGTGATCTCTCTGATACGGAGACTTCTACCAGCTAATGCAAACCATAAACACAATTCGTCGGGTTTTGAGGCCCTTGTGCAAGGATTAAATGCTGATTCCATGGAAAAGAGCGAGCCCACTCCACCTGGAGTCATATCACTGCACTCATCAAAAGATGTATCTGAAAGCATGGACATTATTGACGATTGTCATAAAAATGCTCCGGAGGAAGGAGAAAACGTTGATTTATATGGTGTCCCAGTGGGAATAGAGGCTTGGGAAGAGTATGGTTGCATTTTATGGGATCTTGCTGCAAGTAAAACTCATGCAGAACTCATGGTGCAAAACCTTGTACTTGAAGTGCTTTTAGCAAACCTTATGGTTTCACAATCTGTGCGTGCTATGGAAATTAGCCTTGGAATTATTGGAAACCTTGCCTGTCATGAAGTTCCCATGAAACAAATAATTTCTACAAATGGATTAATTGGAATAATTGTGGATCAATTGTTTTCTGATGATGCTCAGTGTTTATGTGAAATCTGCAGGCTGTTAACTGTGGGTCTTCATAGTAGTGAACGTATTACTTGGGCCAAGGCATTGCAGTCTGAACACAATCTGAGCCGCATTCTATGGATTGCAGAAAATAGCTTAAATCTACAGCTTATAGAAAAGAATGTTGAACTCTTGTTAGCCACCATTGAAAACTCAGACGAAGTTGTGCATATTCTCCTTCCACCTTTAATGAAGCTGGGATTAGCAAGTCTACTCATAATTCTCTTGGATTTCGAAATGAGCAAATTAATGAGTGAAAGAGAACCTGAAAGGTACCCAATTCTTGATGTAATCCTCCGTTCCATTGAAGCTCTCTCAGTTATTGATGGTCATTCCCAACACATCTGTTCAAACAAGAAACTTTTTCAGCTAGCTTGTGACCTAGTCAAACTCCCTGATAAAGTTGAGGTGTCAAATTCTTGTGTTACAGCTGCAATTCTAATTGCGAACATTCTTTCAGATGAATCTACTCTAGCATCGGAGATAGCGGAGGATTTGCCTTTCTTACAGGGTCTGCTTGATTTATTTCCCTTTTCTTCGGACGATTTGGAAGCAAGGAGTGCACTCTGGAACATCATTGCAAGGTTAATGGTTCGAGTTAAGGAAAAGGAAATGAGCTCATCAGTTCTCCAGCAGTATGTTTTGGTTCTAGTCAACAAATCTGATGCGATTGAAGATGATCTTCTTGACTTTCAATTAGGTGACTTACAGTCAAAAGCTAGAACTACAGCTCTTAGAAGGATCGTCAGTATTTTAAATCAATGGACTACTTCAAAGGATTATGCCAAGGATAATGACATGACGGACGAACATGCAGATGATTTAAGTGTTGATAGATTGCTGCACTGCTGTCGTAAGCATTCTGAGCATGAACCGTCTACAGTTTCTCAGGGTACGGAAGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

527

Amino Acids

58.39

Weight (kDa)

4.59

Isoelectric Point (pI)

60.95

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 948
AccB1I GGYRCC 1 cut(s) 948
AccI GTMKAC 2 cut(s) 886, 1553
AccIII TCCGGA 1 cut(s) 346
AciI CCGC 3 cut(s) 78, 745, 1163
AclI AACGTT 1 cut(s) 363
AclWI GGATC 4 cut(s) 128, 435, 630, 1424
AcsI RAATTY 1 cut(s) 1090
AfaI GTAC 3 cut(s) 477, 950, 1570
AfiI CCNNNNNNNGG 1 cut(s) 353
AgsI TTSAA 9 cut(s) 21, 482, 803, 824, 983, 1026, 1349, 1370, 1449
AhdI GACNNNNNGTC 1 cut(s) 1384
AjnI CCWGG 1 cut(s) 268
AjuI GAANNNNNNNTTGG 4 cut(s) 382, 414, 522, 554
Alw21I GWGCWC 2 cut(s) 1240, 1295
Alw26I GTCTC 1 cut(s) 157
Alw44I GTGCAC 1 cut(s) 1236
AlwI GGATC 4 cut(s) 128, 435, 630, 1424
AlwNI CAGNNNCTG 1 cut(s) 1189
Aor13HI TCCGGA 1 cut(s) 346
AoxI GGCC 2 cut(s) 213, 711
ApaLI GTGCAC 1 cut(s) 1236
ApeKI GCWGC 3 cut(s) 434, 1106, 1516
ApoI RAATTY 1 cut(s) 1090
AseI ATTAAT 2 cut(s) 605, 923
Asp718I GGTACC 1 cut(s) 948
AspLEI GCGC 1 cut(s) 7
AspS9I GGNCC 3 cut(s) 34, 214, 711
AsuHPI GGTGA 1 cut(s) 1388
AsuII TTCGAA 1 cut(s) 909
AsuNHI GCTAGC 1 cut(s) 1045
AvaII GGWCC 1 cut(s) 34
BaeGI GKGCMC 1 cut(s) 1240
BanI GGYRCC 1 cut(s) 948
BanII GRGCYC 2 cut(s) 261, 1295
BbsI GAAGAC 1 cut(s) 677
Bbv12I GWGCWC 2 cut(s) 1240, 1295
BbvI GCAGC 3 cut(s) 421, 1093, 1503
BccI CCATC 1 cut(s) 995
BcgI CGANNNNNNTGC 2 cut(s) 1508, 1542
BciT130I CCWGG 1 cut(s) 270
BcoDI GTCTC 1 cut(s) 157
BfaI CTAG 6 cut(s) 138, 1046, 1058, 1148, 1325, 1395
BfmI CTRYAG 5 cut(s) 29, 664, 779, 1401, 1554
BfoI RGCGCY 1 cut(s) 8
BisI GCNGC 4 cut(s) 435, 745, 1107, 1517
BlsI GCNGC 4 cut(s) 436, 746, 1108, 1518
Bme1390I CCNGG 1 cut(s) 270
Bme18I GGWCC 1 cut(s) 34
BmeRI GACNNNNNGTC 1 cut(s) 1384
BmgT120I GGNCC 3 cut(s) 34, 214, 711
BmiI GGNNCC 1 cut(s) 950
BmrFI CCNGG 1 cut(s) 270
BmrI ACTGGG 1 cut(s) 377
BmsI GCATC 3 cut(s) 631, 1160, 1330
BmtI GCTAGC 1 cut(s) 1049
BmuI ACTGGG 1 cut(s) 377
BoxI GACNNNNGTC 1 cut(s) 330
BpiI GAAGAC 1 cut(s) 677
BpmI CTGGAG 2 cut(s) 291, 1289
Bpu14I TTCGAA 1 cut(s) 909
BsaJI CCNNGG 4 cut(s) 243, 538, 714, 1461
BsaWI WCCGGW 1 cut(s) 346
BsaXI ACNNNNNCTCC 2 cut(s) 348, 378
Bsc4I CCNNNNNNNGG 1 cut(s) 353
Bse1I ACTGG 1 cut(s) 383
Bse3DI GCAATG 2 cut(s) 719, 1251
BseAI TCCGGA 1 cut(s) 346
BseBI CCWGG 1 cut(s) 270
BseDI CCNNGG 4 cut(s) 243, 538, 714, 1461
BseLI CCNNNNNNNGG 1 cut(s) 353
BseMI GCAATG 2 cut(s) 719, 1251
BseMII CTCAG 6 cut(s) 659, 731, 843, 1005, 1530, 1577
BseNI ACTGG 1 cut(s) 383
BseSI GKGCMC 1 cut(s) 1240
BseXI GCAGC 3 cut(s) 421, 1093, 1503
BseYI CCCAGC 1 cut(s) 871
BsgI GTGCAG 2 cut(s) 269, 1502
BshFI GGCC 2 cut(s) 215, 713
BshNI GGYRCC 1 cut(s) 948
BsiHKAI GWGCWC 2 cut(s) 1240, 1295
BsiSI CCGG 1 cut(s) 347
BslFI GGGAC 1 cut(s) 365
BslI CCNNNNNNNGG 1 cut(s) 353
BsmAI GTCTC 1 cut(s) 157
BsmFI GGGAC 1 cut(s) 365
BsmI GAATGC 2 cut(s) 747, 1534
BsnI GGCC 2 cut(s) 215, 713
Bsp119I TTCGAA 1 cut(s) 909
Bsp1286I GDGCHC 3 cut(s) 261, 1240, 1295
Bsp13I TCCGGA 1 cut(s) 346
Bsp143I GATC 6 cut(s) 133, 147, 427, 622, 1354, 1416
Bsp19I CCATGG 1 cut(s) 243
BspACI CCGC 3 cut(s) 78, 745, 1163
BspANI GGCC 2 cut(s) 215, 713
BspCNI CTCAG 6 cut(s) 658, 732, 842, 1004, 1531, 1576
BspEI TCCGGA 1 cut(s) 346
BspHI TCATGA 1 cut(s) 565
BspLI GGNNCC 1 cut(s) 950
BspMAI CTGCAG 1 cut(s) 668
BspOI GCTAGC 1 cut(s) 1049
BspPI GGATC 4 cut(s) 128, 435, 630, 1424
BspT104I TTCGAA 1 cut(s) 909
BspT107I GGYRCC 1 cut(s) 948
BsrDI GCAATG 2 cut(s) 719, 1251
BsrI ACTGG 1 cut(s) 383
BssECI CCNNGG 4 cut(s) 243, 538, 714, 1461
BssMI GATC 6 cut(s) 133, 147, 427, 622, 1354, 1416
BssT1I CCWWGG 4 cut(s) 243, 538, 714, 1461
Bst2UI CCWGG 1 cut(s) 270
Bst4CI ACNGT 6 cut(s) 57, 130, 679, 1386, 1551, 1558
Bst6I CTCTTC 1 cut(s) 399
BstAPI GCANNNNNTGC 1 cut(s) 1235
BstBI TTCGAA 1 cut(s) 909
BstC8I GCNNGC 4 cut(s) 257, 522, 668, 1047
BstDEI CTNAG 7 cut(s) 645, 740, 829, 991, 1409, 1539, 1563
BstDSI CCRYGG 1 cut(s) 243
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 1 cut(s) 7
BstKTI GATC 6 cut(s) 136, 150, 430, 625, 1357, 1419
BstMAI GTCTC 1 cut(s) 157
BstMBI GATC 6 cut(s) 133, 147, 427, 622, 1354, 1416
BstMWI GCNNNNNNNGC 2 cut(s) 1235, 1540
BstNI CCWGG 1 cut(s) 270
BstNSI RCATGY 1 cut(s) 1490
BstPAI GACNNNNGTC 1 cut(s) 330
BstSCI CCNGG 1 cut(s) 268
BstSFI CTRYAG 5 cut(s) 29, 664, 779, 1401, 1554
BstSLI GKGCMC 1 cut(s) 1240
BstV1I GCAGC 3 cut(s) 421, 1093, 1503
BstV2I GAAGAC 1 cut(s) 677
BstX2I RGATCY 2 cut(s) 133, 427
BstYI RGATCY 2 cut(s) 133, 427
BsuRI GGCC 2 cut(s) 215, 713
BtgI CCRYGG 1 cut(s) 243
BtsI GCAGTG 2 cut(s) 281, 1519
BtsIMutI CAGTG 4 cut(s) 281, 390, 653, 1519
Cac8I GCNNGC 4 cut(s) 257, 522, 668, 1047
CaiI CAGNNNCTG 1 cut(s) 1189
CciI TCATGA 1 cut(s) 565
CfoI GCGC 1 cut(s) 7
Cfr13I GGNCC 3 cut(s) 34, 214, 711
Csp6I GTAC 3 cut(s) 476, 949, 1569
CviAII CATG 9 cut(s) 244, 313, 449, 460, 566, 577, 1474, 1487, 1544
CviQI GTAC 3 cut(s) 476, 949, 1569
DdeI CTNAG 7 cut(s) 645, 740, 829, 991, 1409, 1539, 1563
DpnI GATC 6 cut(s) 135, 149, 429, 624, 1356, 1418
DpnII GATC 6 cut(s) 133, 147, 427, 622, 1354, 1416
DraI TTTAAA 1 cut(s) 1431
DriI GACNNNNNGTC 1 cut(s) 1384
Eam1104I CTCTTC 1 cut(s) 399
Eam1105I GACNNNNNGTC 1 cut(s) 1384
EarI CTCTTC 1 cut(s) 399
Ecl136II GAGCTC 1 cut(s) 1293
Eco130I CCWWGG 4 cut(s) 243, 538, 714, 1461
Eco24I GRGCYC 2 cut(s) 261, 1295
Eco47I GGWCC 1 cut(s) 34
Eco53kI GAGCTC 1 cut(s) 1293
EcoICRI GAGCTC 1 cut(s) 1293
EcoO109I RGGNCCY 1 cut(s) 214
EcoRII CCWGG 1 cut(s) 268
EcoT14I CCWWGG 4 cut(s) 243, 538, 714, 1461
EcoT38I GRGCYC 2 cut(s) 261, 1295
ErhI CCWWGG 4 cut(s) 243, 538, 714, 1461
FaeI CATG 9 cut(s) 247, 316, 452, 463, 569, 580, 1477, 1490, 1547
FalI AAGNNNNNCTT 2 cut(s) 1341, 1373
FaqI GGGAC 1 cut(s) 365
FatI CATG 9 cut(s) 243, 312, 448, 459, 565, 576, 1473, 1486, 1543
FblI GTMKAC 2 cut(s) 886, 1553
Fnu4HI GCNGC 4 cut(s) 435, 745, 1107, 1517
FriOI GRGCYC 2 cut(s) 261, 1295
Fsp4HI GCNGC 4 cut(s) 435, 745, 1107, 1517
FspBI CTAG 6 cut(s) 138, 1046, 1058, 1148, 1325, 1395
GlaI GCGC 1 cut(s) 6
GluI GCNGC 4 cut(s) 435, 745, 1107, 1517
GsaI CCCAGC 1 cut(s) 875
GsuI CTGGAG 2 cut(s) 291, 1289
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 2 cut(s) 215, 713
HapII CCGG 1 cut(s) 347
HhaI GCGC 1 cut(s) 7
Hin1II CATG 9 cut(s) 247, 316, 452, 463, 569, 580, 1477, 1490, 1547
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HincII GTYRAC 2 cut(s) 675, 1330
HindII GTYRAC 2 cut(s) 675, 1330
HinfI GANTC 4 cut(s) 11, 239, 273, 1139
HpaI GTTAAC 1 cut(s) 675
HpaII CCGG 1 cut(s) 347
HphI GGTGA 1 cut(s) 1388
Hpy166II GTNNAC 6 cut(s) 675, 698, 887, 1238, 1330, 1554
Hpy188III TCNNGA 5 cut(s) 347, 566, 959, 1243, 1361
Hpy8I GTNNAC 6 cut(s) 675, 698, 887, 1238, 1330, 1554
Hpy99I CGWCG 1 cut(s) 205
HpyAV CCTTC 4 cut(s) 99, 347, 863, 1407
HpyCH4III ACNGT 6 cut(s) 57, 130, 679, 1386, 1551, 1558
HpyCH4IV ACGT 2 cut(s) 363, 700
HpyF10VI GCNNNNNNNGC 2 cut(s) 1235, 1540
HpyF3I CTNAG 7 cut(s) 645, 740, 829, 991, 1409, 1539, 1563
HpySE526I ACGT 2 cut(s) 363, 700
Hsp92II CATG 9 cut(s) 247, 316, 452, 463, 569, 580, 1477, 1490, 1547
HspAI GCGC 1 cut(s) 5
Kpn2I TCCGGA 1 cut(s) 346
KpnI GGTACC 1 cut(s) 952
KspAI GTTAAC 1 cut(s) 675
Kzo9I GATC 6 cut(s) 133, 147, 427, 622, 1354, 1416
LmnI GCTCC 2 cut(s) 100, 349
Lsp1109I GCAGC 3 cut(s) 421, 1093, 1503
LweI GCATC 3 cut(s) 631, 1160, 1330
MaeI CTAG 6 cut(s) 138, 1046, 1058, 1148, 1325, 1395
MaeII ACGT 2 cut(s) 363, 700
MaeIII GTNAC 4 cut(s) 57, 1052, 1099, 1376
MalI GATC 6 cut(s) 135, 149, 429, 624, 1356, 1418
MboI GATC 6 cut(s) 133, 147, 427, 622, 1354, 1416
MboII GAAGA 6 cut(s) 53, 416, 677, 1203, 1349, 1361
MfeI CAATTG 1 cut(s) 626
MflI RGATCY 2 cut(s) 133, 427
MhlI GDGCHC 3 cut(s) 261, 1240, 1295
MlyI GAGTC 2 cut(s) 5, 282
MnlI CCTC 7 cut(s) 36, 205, 343, 388, 980, 1075, 1159
MroI TCCGGA 1 cut(s) 346
MslI CAYNNNNRTG 3 cut(s) 339, 693, 1491
MspA1I CMGCKG 1 cut(s) 1106
MspI CCGG 1 cut(s) 347
MspR9I CCNGG 1 cut(s) 270
MunI CAATTG 1 cut(s) 626
Mva1269I GAATGC 2 cut(s) 747, 1534
MvaI CCWGG 1 cut(s) 270
MwoI GCNNNNNNNGC 2 cut(s) 1235, 1540
NcoI CCATGG 1 cut(s) 243
NdeII GATC 6 cut(s) 133, 147, 427, 622, 1354, 1416
NheI GCTAGC 1 cut(s) 1045
NlaIII CATG 9 cut(s) 247, 316, 452, 463, 569, 580, 1477, 1490, 1547
NlaIV GGNNCC 1 cut(s) 950
NmuCI GTSAC 2 cut(s) 1052, 1376
NspI RCATGY 1 cut(s) 1490
NspV TTCGAA 1 cut(s) 909
PagI TCATGA 1 cut(s) 565
PctI GAATGC 2 cut(s) 747, 1534
PfeI GAWTC 2 cut(s) 239, 1139
PflFI GACNNNGTC 1 cut(s) 1058
PkrI GCNGC 4 cut(s) 436, 746, 1108, 1518
PleI GAGTC 2 cut(s) 5, 281
PpsI GAGTC 2 cut(s) 5, 281
PshAI GACNNNNGTC 1 cut(s) 330
PshBI ATTAAT 2 cut(s) 605, 923
Psp124BI GAGCTC 1 cut(s) 1295
Psp1406I AACGTT 1 cut(s) 363
Psp6I CCWGG 1 cut(s) 268
PspFI CCCAGC 1 cut(s) 871
PspGI CCWGG 1 cut(s) 268
PspN4I GGNNCC 1 cut(s) 950
PspPI GGNCC 3 cut(s) 34, 214, 711
PsrI GAACNNNNNNTAC 2 cut(s) 42, 74
PstI CTGCAG 1 cut(s) 668
PstNI CAGNNNCTG 1 cut(s) 1189
PsuI RGATCY 2 cut(s) 133, 427
PsyI GACNNNGTC 1 cut(s) 1058
PvuII CAGCTG 1 cut(s) 1106
RsaI GTAC 3 cut(s) 477, 950, 1570
RsaNI GTAC 3 cut(s) 476, 949, 1569
RseI CAYNNNNRTG 3 cut(s) 339, 693, 1491
SacI GAGCTC 1 cut(s) 1295
SatI GCNGC 4 cut(s) 435, 745, 1107, 1517
Sau3AI GATC 6 cut(s) 133, 147, 427, 622, 1354, 1416
Sau96I GGNCC 3 cut(s) 34, 214, 711
SchI GAGTC 2 cut(s) 5, 282
ScrFI CCNGG 1 cut(s) 270
SduI GDGCHC 3 cut(s) 261, 1240, 1295
SfaNI GCATC 3 cut(s) 631, 1160, 1330
SfcI CTRYAG 5 cut(s) 29, 664, 779, 1401, 1554
SfuI TTCGAA 1 cut(s) 909
SinI GGWCC 1 cut(s) 34
SmiMI CAYNNNNRTG 3 cut(s) 339, 693, 1491
SsiI CCGC 3 cut(s) 78, 745, 1163
SspMI CTAG 6 cut(s) 138, 1046, 1058, 1148, 1325, 1395
SstI GAGCTC 1 cut(s) 1295
StyD4I CCNGG 1 cut(s) 268
StyI CCWWGG 4 cut(s) 243, 538, 714, 1461
TaaI ACNGT 6 cut(s) 57, 130, 679, 1386, 1551, 1558
TaiI ACGT 2 cut(s) 366, 703
TaqI TCGA 2 cut(s) 909, 1270
TatI WGTACW 1 cut(s) 475
TauI GCSGC 1 cut(s) 747
TfiI GAWTC 2 cut(s) 239, 1139
TscAI CASTG 4 cut(s) 288, 390, 653, 1526
TseFI GTSAC 2 cut(s) 1052, 1376
TseI GCWGC 3 cut(s) 434, 1106, 1516
Tsp45I GTSAC 2 cut(s) 1052, 1376
TspDTI ATGAA 6 cut(s) 582, 593, 677, 881, 1152, 1560
TspGWI ACGGA 3 cut(s) 175, 962, 1493
TspRI CASTG 4 cut(s) 288, 390, 653, 1526
Tth111I GACNNNGTC 1 cut(s) 1058
VneI GTGCAC 1 cut(s) 1236
VpaK11BI GGWCC 1 cut(s) 34
VspI ATTAAT 2 cut(s) 605, 923
XapI RAATTY 1 cut(s) 1090
XceI RCATGY 1 cut(s) 1490
XmiI GTMKAC 2 cut(s) 886, 1553
XspI CTAG 6 cut(s) 138, 1046, 1058, 1148, 1325, 1395
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.