Rw5G034470

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000928
Physical Location & Seq
Reverse (-)
59415899 .. 59422840
6942 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G034470.1

Sequence Viewer

Length: 1596 bp
ATGGCGCCGGACTCAAAATCAGCACCCCTACAACACCAGGAAGAAGAAGAACCACAACCGGAAGACCTCGTCACACTTTCACACAACCCTTCTGCTCCGCCTGATGAGTTATTCGACATCTCAACGACAGTAGATCCGAGTTATGTGATCTCTCTGATACGGAAACTTCTACCAGCTAATGCCAGTAATCATCACAACTCTCGCGGTGAGGTTTCATGTGGCCCTGTGCAAGGATTAAATGTTGATGACATGGAAAAAAGTGCTCTAACTCGCTCTGTTGTTCCACCCCCATCAACGGACACATCTGAGAGCATGGAAATTAATGACGATTTTAATGAAAATGCTACCCATGGAGGAGAAAGTGAAGCTGAACAGCCTGGTCATAGTGTGCCGGTGGGAGAAGAGGCTTGGGAAGAGTATGGTTGCATATTATGGGATCTTTCTGCAAGTAAAACTCATGCAGAACTCATGGTGCAGAACCTTGTACTTGAAGTGCTGTTAGCAAACCTTATGGTTTCACAATCCGTTCGTACTACGGAAATTGGTCTCGGAATCATCGGAAACCTTGCCTGTCACGAAGTTCCCATGAAACATATAGTCTCTACTAATGGATTAACTGAATTCATTGTGGATCAGATGTTTCTGGATGATGCTCAATGCTTATGTGAAGTCTGCAGACTGTTAACTGTGGGTCTTCAAAGTAGTGAAGGTGTTACTTGGGCTGAGGCATTGCAGTCAGAACATTATCTGACCCACATTTTATGGATTGCAGAAAATAGCTTGAACACACAGCTTATAGAAAAGAGTGCTGAACTATTGTTAGCGATCATCGAAAGTTCGCAGGATGTTGCGCATATTCTCTTACCGCCATTGATGAAGCTGGGCTTAGCAAGTCTATTAATAAATCTCTTGGATATTGAAACAAGCAAATTAATGAGTGAAAGAGCTCCTGAAAGGTACCCAATTCTTGATGTAGTTCTTCGTGCCATTGAAGCTCTTTCTGTTATTGATGGTCATTCCCAGGACATCTGTTTAGACAAGGATCTCTTTCAGCTGGTTTGTGCCTTGCTCAAGTTCCCTGATAAAGTTGAGGTTGCAAACTCCTGTGTTACAGCTGTAGTTCTAGTTGCAAATATTCTTTCAGACGTACCTACTCTAGCTTCAGAGTTATCACATGATATGCTTTTCTTACAGGGTCTGCTTGATGTGTTTCCTTTTACCGCGGATGACTCAGAAGCAAGGAGTGCACTGTGGAACATCATTGCAAGGTTACTGGTTCGAGTTCCAGAAAATGAAATGAGCTCATCAACTCTCCAGAAGTACGTTTTGGTTCTAGTTTGCAAAACTGATGTGATTGAAGATGATCTTCTTGACTGCCAGTTAGGTGGCTTAGGATTAAAGGCTAGAACTACAGTTGTATCCTGTTCTGTGAATTTTTTGTTGAACTTGCAGTTAAAATGCAATAAAGTTGGCAGTTTGAATCTTATTCCAGCAATATCTAAGGTCAATGCTGTTTCCCTTCTCAAGTCCTGTCTTGCAATATATTCCGTTGTCCTTTACTTTTGCTACAGCTCAGAAGGATCATCAGTATTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

531

Amino Acids

57.91

Weight (kDa)

4.46

Isoelectric Point (pI)

57.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 852
Acc65I GGTACC 1 cut(s) 957
AccB1I GGYRCC 2 cut(s) 4, 957
AccII CGCG 2 cut(s) 204, 1223
AciI CCGC 5 cut(s) 98, 204, 866, 1221, 1223
AclWI GGATC 5 cut(s) 128, 444, 639, 1050, 1588
AcsI RAATTY 2 cut(s) 620, 1432
AcuI CTGAAG 1 cut(s) 1146
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 5 cut(s) 486, 532, 959, 1149, 1322
AfiI CCNNNNNNNGG 2 cut(s) 230, 295
AgsI TTSAA 8 cut(s) 491, 698, 784, 920, 992, 1358, 1444, 1480
AjnI CCWGG 3 cut(s) 36, 376, 1020
AjuI GAANNNNNNNTTGG 2 cut(s) 1310, 1342
Alw21I GWGCWC 4 cut(s) 265, 949, 1249, 1304
Alw26I GTCTC 2 cut(s) 551, 604
Alw44I GTGCAC 1 cut(s) 1245
AlwI GGATC 5 cut(s) 128, 444, 639, 1050, 1588
AlwNI CAGNNNCTG 1 cut(s) 1198
AoxI GGCC 1 cut(s) 220
ApaLI GTGCAC 1 cut(s) 1245
ApoI RAATTY 2 cut(s) 620, 1432
AseI ATTAAT 3 cut(s) 321, 899, 932
Asp718I GGTACC 1 cut(s) 957
AspLEI GCGC 2 cut(s) 7, 853
AspS9I GGNCC 1 cut(s) 221
AsuHPI GGTGA 1 cut(s) 218
BaeGI GKGCMC 1 cut(s) 1249
BaeI ACNNNNGTAYC 2 cut(s) 1401, 1434
BanI GGYRCC 2 cut(s) 4, 957
BanII GRGCYC 2 cut(s) 949, 1304
BbsI GAAGAC 2 cut(s) 69, 686
Bbv12I GWGCWC 4 cut(s) 265, 949, 1249, 1304
BbvCI CCTCAGC 1 cut(s) 723
BccI CCATC 2 cut(s) 298, 1004
BciT130I CCWGG 3 cut(s) 38, 378, 1022
BciVI GTATCC 1 cut(s) 1429
BcoDI GTCTC 2 cut(s) 551, 604
BfaI CTAG 4 cut(s) 1124, 1157, 1334, 1404
BfmI CTRYAG 4 cut(s) 673, 1116, 1410, 1567
BfoI RGCGCY 1 cut(s) 8
BfuI GTATCC 1 cut(s) 1429
BlpI GCTNAGC 1 cut(s) 886
Bme1390I CCNGG 3 cut(s) 38, 378, 1022
BmgT120I GGNCC 1 cut(s) 221
BmiI GGNNCC 2 cut(s) 6, 959
BmrFI CCNGG 3 cut(s) 38, 378, 1022
BmsI GCATC 1 cut(s) 640
BpiI GAAGAC 2 cut(s) 69, 686
BpmI CTGGAG 1 cut(s) 1298
Bpu10I CCTNAGC 2 cut(s) 723, 1390
Bpu1102I GCTNAGC 1 cut(s) 886
BpuEI CTTGAG 2 cut(s) 1055, 1508
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 551
BsaJI CCNNGG 3 cut(s) 349, 1020, 1221
BsaWI WCCGGW 1 cut(s) 58
Bsc4I CCNNNNNNNGG 2 cut(s) 230, 295
Bse118I RCCGGY 1 cut(s) 391
Bse1I ACTGG 3 cut(s) 183, 1278, 1378
Bse3DI GCAATG 2 cut(s) 728, 1260
BseBI CCWGG 3 cut(s) 38, 378, 1022
BseDI CCNNGG 3 cut(s) 349, 1020, 1221
BseGI GGATG 3 cut(s) 652, 850, 1231
BseLI CCNNNNNNNGG 2 cut(s) 230, 295
BseMI GCAATG 2 cut(s) 728, 1260
BseMII CTCAG 4 cut(s) 297, 714, 1245, 1587
BseNI ACTGG 3 cut(s) 183, 1278, 1378
BseRI GAGGAG 1 cut(s) 369
BseSI GKGCMC 1 cut(s) 1249
BseYI CCCAGC 1 cut(s) 880
BsgI GTGCAG 1 cut(s) 494
Bsh1236I CGCG 2 cut(s) 204, 1223
BshFI GGCC 1 cut(s) 222
BshNI GGYRCC 2 cut(s) 4, 957
BsiHKAI GWGCWC 4 cut(s) 265, 949, 1249, 1304
BsiSI CCGG 3 cut(s) 8, 59, 392
BslI CCNNNNNNNGG 2 cut(s) 230, 295
BsmAI GTCTC 2 cut(s) 551, 604
BsnI GGCC 1 cut(s) 222
Bso31I GGTCTC 1 cut(s) 551
Bsp1286I GDGCHC 4 cut(s) 265, 949, 1249, 1304
Bsp143I GATC 8 cut(s) 133, 147, 436, 631, 825, 1042, 1363, 1580
Bsp1720I GCTNAGC 1 cut(s) 886
Bsp19I CCATGG 1 cut(s) 349
BspACI CCGC 5 cut(s) 98, 204, 866, 1221, 1223
BspANI GGCC 1 cut(s) 222
BspCNI CTCAG 4 cut(s) 298, 715, 1244, 1586
BspFNI CGCG 2 cut(s) 204, 1223
BspLI GGNNCC 2 cut(s) 6, 959
BspMAI CTGCAG 1 cut(s) 677
BspPI GGATC 5 cut(s) 128, 444, 639, 1050, 1588
BspT107I GGYRCC 2 cut(s) 4, 957
BspTNI GGTCTC 1 cut(s) 551
BsrDI GCAATG 2 cut(s) 728, 1260
BsrFI RCCGGY 1 cut(s) 391
BsrI ACTGG 3 cut(s) 183, 1278, 1378
BssAI RCCGGY 1 cut(s) 391
BssECI CCNNGG 3 cut(s) 349, 1020, 1221
BssMI GATC 8 cut(s) 133, 147, 436, 631, 825, 1042, 1363, 1580
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 1 cut(s) 349
Bst2UI CCWGG 3 cut(s) 38, 378, 1022
Bst4CI ACNGT 5 cut(s) 130, 681, 688, 1251, 1414
Bst6I CTCTTC 2 cut(s) 396, 408
BstACI GRCGYC 1 cut(s) 5
BstAPI GCANNNNNTGC 1 cut(s) 1244
BstDEI CTNAG 7 cut(s) 306, 723, 886, 1231, 1390, 1500, 1573
BstDSI CCRYGG 2 cut(s) 349, 1221
BstENI CCTNNNNNAGG 1 cut(s) 228
BstF5I GGATG 3 cut(s) 652, 850, 1231
BstFNI CGCG 2 cut(s) 204, 1223
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 2 cut(s) 7, 853
BstKTI GATC 8 cut(s) 136, 150, 439, 634, 828, 1045, 1366, 1583
BstMAI GTCTC 2 cut(s) 551, 604
BstMBI GATC 8 cut(s) 133, 147, 436, 631, 825, 1042, 1363, 1580
BstMWI GCNNNNNNNGC 2 cut(s) 992, 1244
BstNI CCWGG 3 cut(s) 38, 378, 1022
BstSCI CCNGG 3 cut(s) 36, 376, 1020
BstSFI CTRYAG 4 cut(s) 673, 1116, 1410, 1567
BstSLI GKGCMC 1 cut(s) 1249
BstUI CGCG 2 cut(s) 204, 1223
BstV2I GAAGAC 2 cut(s) 69, 686
BstX2I RGATCY 3 cut(s) 133, 436, 1042
BstXI CCANNNNNNTGG 1 cut(s) 1385
BstYI RGATCY 3 cut(s) 133, 436, 1042
BsuI GTATCC 1 cut(s) 1429
BsuRI GGCC 1 cut(s) 222
BtgI CCRYGG 2 cut(s) 349, 1221
BtsCI GGATG 3 cut(s) 652, 850, 1231
BtsIMutI CAGTG 1 cut(s) 1247
CaiI CAGNNNCTG 1 cut(s) 1198
CfoI GCGC 2 cut(s) 7, 853
Cfr10I RCCGGY 1 cut(s) 391
Cfr13I GGNCC 1 cut(s) 221
Cfr42I CCGCGG 1 cut(s) 1224
Csp6I GTAC 5 cut(s) 485, 531, 958, 1148, 1321
CviAII CATG 8 cut(s) 216, 250, 313, 350, 458, 469, 586, 1175
CviQI GTAC 5 cut(s) 485, 531, 958, 1148, 1321
DdeI CTNAG 7 cut(s) 306, 723, 886, 1231, 1390, 1500, 1573
DinI GGCGCC 1 cut(s) 6
DpnI GATC 8 cut(s) 135, 149, 438, 633, 827, 1044, 1365, 1582
DpnII GATC 8 cut(s) 133, 147, 436, 631, 825, 1042, 1363, 1580
Eam1104I CTCTTC 2 cut(s) 396, 408
EarI CTCTTC 2 cut(s) 396, 408
EciI GGCGGA 1 cut(s) 87
Ecl136II GAGCTC 2 cut(s) 947, 1302
Eco130I CCWWGG 1 cut(s) 349
Eco24I GRGCYC 2 cut(s) 949, 1304
Eco31I GGTCTC 1 cut(s) 551
Eco53kI GAGCTC 2 cut(s) 947, 1302
Eco57I CTGAAG 1 cut(s) 1146
EcoICRI GAGCTC 2 cut(s) 947, 1302
EcoNI CCTNNNNNAGG 1 cut(s) 228
EcoRI GAATTC 1 cut(s) 620
EcoRII CCWGG 3 cut(s) 36, 376, 1020
EcoT14I CCWWGG 1 cut(s) 349
EcoT38I GRGCYC 2 cut(s) 949, 1304
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 349
FaeI CATG 8 cut(s) 219, 253, 316, 353, 461, 472, 589, 1178
FalI AAGNNNNNCTT 6 cut(s) 869, 901, 1031, 1063, 1350, 1382
FatI CATG 8 cut(s) 215, 249, 312, 349, 457, 468, 585, 1174
FokI GGATG 3 cut(s) 659, 857, 1238
FriOI GRGCYC 2 cut(s) 949, 1304
FspBI CTAG 4 cut(s) 1124, 1157, 1334, 1404
FspI TGCGCA 1 cut(s) 852
GlaI GCGC 2 cut(s) 6, 852
GsaI CCCAGC 1 cut(s) 884
GsuI CTGGAG 1 cut(s) 1298
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 1 cut(s) 222
HapII CCGG 3 cut(s) 8, 59, 392
HhaI GCGC 2 cut(s) 7, 853
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 8 cut(s) 219, 253, 316, 353, 461, 472, 589, 1178
Hin6I GCGC 2 cut(s) 5, 851
HinP1I GCGC 2 cut(s) 5, 851
HincII GTYRAC 1 cut(s) 684
HindII GTYRAC 1 cut(s) 684
HinfI GANTC 4 cut(s) 11, 552, 1229, 1480
HpaI GTTAAC 1 cut(s) 684
HpaII CCGG 3 cut(s) 8, 59, 392
HphI GGTGA 1 cut(s) 218
Hpy166II GTNNAC 2 cut(s) 684, 1247
Hpy188III TCNNGA 7 cut(s) 575, 644, 950, 968, 1286, 1315, 1370
Hpy8I GTNNAC 2 cut(s) 684, 1247
HpyAV CCTTC 4 cut(s) 99, 701, 1529, 1571
HpyCH4III ACNGT 5 cut(s) 130, 681, 688, 1251, 1414
HpyCH4IV ACGT 2 cut(s) 1146, 1323
HpyF10VI GCNNNNNNNGC 2 cut(s) 992, 1244
HpyF3I CTNAG 7 cut(s) 306, 723, 886, 1231, 1390, 1500, 1573
HpySE526I ACGT 2 cut(s) 1146, 1323
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 8 cut(s) 219, 253, 316, 353, 461, 472, 589, 1178
HspAI GCGC 2 cut(s) 5, 851
KasI GGCGCC 1 cut(s) 4
KpnI GGTACC 1 cut(s) 961
KspAI GTTAAC 1 cut(s) 684
KspI CCGCGG 1 cut(s) 1224
Kzo9I GATC 8 cut(s) 133, 147, 436, 631, 825, 1042, 1363, 1580
LmnI GCTCC 2 cut(s) 100, 952
LweI GCATC 1 cut(s) 640
MaeI CTAG 4 cut(s) 1124, 1157, 1334, 1404
MaeII ACGT 2 cut(s) 1146, 1323
MaeIII GTNAC 5 cut(s) 70, 572, 712, 1108, 1269
MalI GATC 8 cut(s) 135, 149, 438, 633, 827, 1044, 1365, 1582
MboI GATC 8 cut(s) 133, 147, 436, 631, 825, 1042, 1363, 1580
MflI RGATCY 3 cut(s) 133, 436, 1042
MhlI GDGCHC 4 cut(s) 265, 949, 1249, 1304
MluCI AATT 6 cut(s) 318, 540, 620, 929, 963, 1432
Mly113I GGCGCC 1 cut(s) 5
MlyI GAGTC 2 cut(s) 5, 1223
MnlI CCTC 6 cut(s) 77, 202, 347, 397, 718, 1084
MspA1I CMGCKG 3 cut(s) 1054, 1115, 1223
MspI CCGG 3 cut(s) 8, 59, 392
MspR9I CCNGG 3 cut(s) 38, 378, 1022
MvaI CCWGG 3 cut(s) 38, 378, 1022
MvnI CGCG 2 cut(s) 204, 1223
MwoI GCNNNNNNNGC 2 cut(s) 992, 1244
NarI GGCGCC 1 cut(s) 5
NcoI CCATGG 1 cut(s) 349
NdeII GATC 8 cut(s) 133, 147, 436, 631, 825, 1042, 1363, 1580
NlaIII CATG 8 cut(s) 219, 253, 316, 353, 461, 472, 589, 1178
NlaIV GGNNCC 2 cut(s) 6, 959
NmuCI GTSAC 2 cut(s) 70, 572
NsbI TGCGCA 1 cut(s) 852
PfeI GAWTC 2 cut(s) 552, 1480
PflFI GACNNNGTC 1 cut(s) 68
PleI GAGTC 2 cut(s) 5, 1223
PluTI GGCGCC 1 cut(s) 8
PpsI GAGTC 2 cut(s) 5, 1223
PshBI ATTAAT 3 cut(s) 321, 899, 932
Psp124BI GAGCTC 2 cut(s) 949, 1304
Psp6I CCWGG 3 cut(s) 36, 376, 1020
PspFI CCCAGC 1 cut(s) 880
PspGI CCWGG 3 cut(s) 36, 376, 1020
PspN4I GGNNCC 2 cut(s) 6, 959
PspPI GGNCC 1 cut(s) 221
PstI CTGCAG 1 cut(s) 677
PstNI CAGNNNCTG 1 cut(s) 1198
PsuI RGATCY 3 cut(s) 133, 436, 1042
PsyI GACNNNGTC 1 cut(s) 68
PvuII CAGCTG 2 cut(s) 1054, 1115
RsaI GTAC 5 cut(s) 486, 532, 959, 1149, 1322
RsaNI GTAC 5 cut(s) 485, 531, 958, 1148, 1321
SacI GAGCTC 2 cut(s) 949, 1304
SacII CCGCGG 1 cut(s) 1224
Sau3AI GATC 8 cut(s) 133, 147, 436, 631, 825, 1042, 1363, 1580
Sau96I GGNCC 1 cut(s) 221
SchI GAGTC 2 cut(s) 5, 1223
ScrFI CCNGG 3 cut(s) 38, 378, 1022
SduI GDGCHC 4 cut(s) 265, 949, 1249, 1304
SfaNI GCATC 1 cut(s) 640
SfcI CTRYAG 4 cut(s) 673, 1116, 1410, 1567
SfoI GGCGCC 1 cut(s) 6
Sfr303I CCGCGG 1 cut(s) 1224
SgrBI CCGCGG 1 cut(s) 1224
SmlI CTYRAG 2 cut(s) 1070, 1523
SmoI CTYRAG 2 cut(s) 1070, 1523
Sse9I AATT 6 cut(s) 318, 540, 620, 929, 963, 1432
SsiI CCGC 5 cut(s) 98, 204, 866, 1221, 1223
SspDI GGCGCC 1 cut(s) 4
SspI AATATT 1 cut(s) 1135
SspMI CTAG 4 cut(s) 1124, 1157, 1334, 1404
SstI GAGCTC 2 cut(s) 949, 1304
StyD4I CCNGG 3 cut(s) 36, 376, 1020
StyI CCWWGG 1 cut(s) 349
TaaI ACNGT 5 cut(s) 130, 681, 688, 1251, 1414
TaiI ACGT 2 cut(s) 1149, 1326
TaqI TCGA 3 cut(s) 114, 831, 1279
TasI AATT 6 cut(s) 318, 540, 620, 929, 963, 1432
TatI WGTACW 1 cut(s) 484
TfiI GAWTC 2 cut(s) 552, 1480
TscAI CASTG 1 cut(s) 1254
TseFI GTSAC 2 cut(s) 70, 572
Tsp45I GTSAC 2 cut(s) 70, 572
TspDTI ATGAA 6 cut(s) 204, 351, 602, 613, 890, 1308
TspGWI ACGGA 5 cut(s) 175, 311, 514, 551, 1537
TspRI CASTG 1 cut(s) 1254
Tth111I GACNNNGTC 1 cut(s) 68
VneI GTGCAC 1 cut(s) 1245
VspI ATTAAT 3 cut(s) 321, 899, 932
XagI CCTNNNNNAGG 1 cut(s) 228
XapI RAATTY 2 cut(s) 620, 1432
XspI CTAG 4 cut(s) 1124, 1157, 1334, 1404
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.