RLG00000035033

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000928
Physical Location & Seq
Reverse (-)
59599907 .. 59606526
6620 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000035033

Sequence Viewer

Length: 1578 bp
ATGGCGCCGGACTCAAAATCAACATCCCTACAACACCAGGAAGAAGAAGAACCACAACCGGAAGACCTTGACGCACTTTCACACAACCCTTCTGCTCCGCCTGATGAGTTATTTGACATCTCAACGACAGTAGATCCGAGTTATGTCATCTCTCTGATACGGAAACTTCTACCAGCTAATGCCAGAAATGATCACAGCTCTCACAGTGATGTTTCTCATGGCCCCGTGCAAGGATTAAATGTTGATGACATGGAGAAAAGTGCTCTTACTCCCACTGATGTTCCACCCTCATCAACAGACACATCTGAGAGCATGGAAATTAATGACGACTTTAATGAAAATGCTACCCGTGAAGGAGAAAATGAAGCCGAACAGCCTGGTCATGGTGTGCCGGTGGGAGAAGAGACTTGGGAAGAGTATGGTTGCATATTATGGGATCTTTCTGCAAGTAAAACTCATGCAGAACTCATGGTGCAGAACCTTGTACTTGAAGTGCTGTTAGCAAACCTAATGGTTTCACAATCCGTTCGTACTCTGGAAATTGGTCTCGGAATCATCGGAAACCTTGCCTGTCACGAAGTTCCCATGAAACATATAGTCTCTACTAATGGATTAATTGAATTCATTGTGGATCAGATGTTTCAGGATGATGCTCAATGCTTATGTGAAGTCTGCAGACTGTTAACTGTGGGTCTTCAAAGTAGTGAAGGTATTACTTCGGCTGAGGCATTGCAGTCGGAACATTATCTGACCCACATTTTATGGATTGCAGAAAATAGCCTGAACACACAGCTTATAGAAAAGAGTGCTGAACTATTGTTAGCGATCATCGAAAGTTCGCAGGATGTTGCACATATTCTCTTACCACCATTGATGAAGCTGGGCTTAGCAAGTCTATTAATAAATCTCTTGGATATTGAAACAAGCAAATTAATGAGTGAGAGAGCCCCTGAAAGGTACCCAATTCTTGATGTAGTTCTACGTGCCATTGAAGCTCTTTCTGTTATTGATGGTCATTCCCAGGACATCTGTTTAAACAAGGATCTCTTTCAGCTGGTTTGTGCCGTGCTCAAGTTCCCTGATAAAGTTGAGGTTGCAAATTCTTGTGTTACAGCTGTAGTTCTTGTTGCAAATATTCTTTCAGATGTACCTACTCTAGCTTCAGAGTTATTACATGATATGCTTTTCTTACAGGGTCTGCTTGATGTGTTTCCCTTTACTTCGGATGACTCAGAAGCAAGGAGTGCACTGTGGAACGTCATTGCAAGGCTACTGGTTCGAGTTCCTGAAAATGAAATGAGCTCATCAACTCTCCAGCAGTACGTTTTGGTTCTAGTTAGCAAATCTGATGTGATTGAAGATGATCTTCTTGACTGCCAGTTAGGTGGCTTAGGATTAAAGGCTAGAACTATAGCTCTCAGAAGGATCATCAGTATTTTAAACCAGTGGACTGCTTCAAAAGATTATGCCAAGGAGAATGAAAATAATATAAACATTGATAGATTGTTGGATTGCTGCTGTAAACATTCTGAAGATGTACCATCTACAGTTTCTCATGACATGGACCTCAAAGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

526

Amino Acids

57.79

Weight (kDa)

4.39

Isoelectric Point (pI)

57.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 957
AccB1I GGYRCC 2 cut(s) 4, 957
AciI CCGC 1 cut(s) 98
AclWI GGATC 5 cut(s) 128, 444, 639, 1050, 1433
AcsI RAATTY 2 cut(s) 620, 1099
AcuI CTGAAG 2 cut(s) 1146, 1551
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 6 cut(s) 486, 532, 959, 1149, 1322, 1539
AfiI CCNNNNNNNGG 3 cut(s) 230, 383, 954
AgsI TTSAA 7 cut(s) 491, 620, 698, 920, 992, 1358, 1458
AjnI CCWGG 3 cut(s) 36, 376, 1020
Alw21I GWGCWC 4 cut(s) 265, 1071, 1249, 1304
Alw26I GTCTC 3 cut(s) 398, 551, 604
Alw44I GTGCAC 1 cut(s) 1245
AlwI GGATC 5 cut(s) 128, 444, 639, 1050, 1433
AlwNI CAGNNNCTG 1 cut(s) 1198
AoxI GGCC 1 cut(s) 220
ApaLI GTGCAC 1 cut(s) 1245
ApeKI GCWGC 1 cut(s) 1515
ApoI RAATTY 2 cut(s) 620, 1099
AseI ATTAAT 4 cut(s) 321, 614, 899, 932
Asp718I GGTACC 1 cut(s) 957
AspLEI GCGC 1 cut(s) 7
AspS9I GGNCC 2 cut(s) 221, 1564
AvaII GGWCC 1 cut(s) 1564
BaeGI GKGCMC 1 cut(s) 1249
BanI GGYRCC 2 cut(s) 4, 957
BanII GRGCYC 2 cut(s) 949, 1304
BbsI GAAGAC 2 cut(s) 69, 686
Bbv12I GWGCWC 4 cut(s) 265, 1071, 1249, 1304
BbvCI CCTCAGC 1 cut(s) 723
BbvI GCAGC 1 cut(s) 1502
BccI CCATC 2 cut(s) 1004, 1549
BceAI ACGGC 1 cut(s) 1049
BcgI CGANNNNNNTGC 2 cut(s) 717, 751
BciT130I CCWGG 3 cut(s) 38, 378, 1022
BclI TGATCA 1 cut(s) 190
BcoDI GTCTC 3 cut(s) 398, 551, 604
BfaI CTAG 3 cut(s) 1157, 1334, 1404
BfmI CTRYAG 4 cut(s) 673, 1116, 1410, 1545
BfoI RGCGCY 1 cut(s) 8
BisI GCNGC 1 cut(s) 1516
BlpI GCTNAGC 1 cut(s) 886
BlsI GCNGC 1 cut(s) 1517
Bme1390I CCNGG 3 cut(s) 38, 378, 1022
Bme18I GGWCC 1 cut(s) 1564
BmgT120I GGNCC 2 cut(s) 221, 1564
BmiI GGNNCC 3 cut(s) 6, 223, 959
BmrFI CCNGG 3 cut(s) 38, 378, 1022
BmsI GCATC 1 cut(s) 640
BpiI GAAGAC 2 cut(s) 69, 686
BpmI CTGGAG 1 cut(s) 1298
Bpu10I CCTNAGC 2 cut(s) 723, 1390
Bpu1102I GCTNAGC 1 cut(s) 886
BpuEI CTTGAG 1 cut(s) 1055
BsaAI YACGTR 1 cut(s) 983
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 551
BsaJI CCNNGG 2 cut(s) 1020, 1470
BsaWI WCCGGW 1 cut(s) 58
Bsc4I CCNNNNNNNGG 3 cut(s) 230, 383, 954
Bse118I RCCGGY 1 cut(s) 391
Bse1I ACTGG 3 cut(s) 1278, 1378, 1444
Bse3DI GCAATG 2 cut(s) 728, 1260
BseBI CCWGG 3 cut(s) 38, 378, 1022
BseDI CCNNGG 2 cut(s) 1020, 1470
BseGI GGATG 4 cut(s) 23, 652, 850, 1231
BseLI CCNNNNNNNGG 3 cut(s) 230, 383, 954
BseMI GCAATG 2 cut(s) 728, 1260
BseMII CTCAG 4 cut(s) 297, 714, 1245, 1432
BseNI ACTGG 3 cut(s) 1278, 1378, 1444
BseSI GKGCMC 1 cut(s) 1249
BseXI GCAGC 1 cut(s) 1502
BseYI CCCAGC 1 cut(s) 880
BsgI GTGCAG 1 cut(s) 494
BshFI GGCC 1 cut(s) 222
BshNI GGYRCC 2 cut(s) 4, 957
BsiHKAI GWGCWC 4 cut(s) 265, 1071, 1249, 1304
BsiSI CCGG 3 cut(s) 8, 59, 392
BslI CCNNNNNNNGG 3 cut(s) 230, 383, 954
BsmAI GTCTC 3 cut(s) 398, 551, 604
BsnI GGCC 1 cut(s) 222
Bso31I GGTCTC 1 cut(s) 551
Bsp1286I GDGCHC 5 cut(s) 265, 949, 1071, 1249, 1304
Bsp143I GATC 8 cut(s) 133, 190, 436, 631, 825, 1042, 1363, 1425
Bsp1720I GCTNAGC 1 cut(s) 886
BspACI CCGC 1 cut(s) 98
BspANI GGCC 1 cut(s) 222
BspCNI CTCAG 4 cut(s) 298, 715, 1244, 1431
BspHI TCATGA 1 cut(s) 1555
BspLI GGNNCC 3 cut(s) 6, 223, 959
BspMAI CTGCAG 1 cut(s) 677
BspPI GGATC 5 cut(s) 128, 444, 639, 1050, 1433
BspT107I GGYRCC 2 cut(s) 4, 957
BspTNI GGTCTC 1 cut(s) 551
BsrDI GCAATG 2 cut(s) 728, 1260
BsrFI RCCGGY 1 cut(s) 391
BsrI ACTGG 3 cut(s) 1278, 1378, 1444
BssAI RCCGGY 1 cut(s) 391
BssECI CCNNGG 2 cut(s) 1020, 1470
BssMI GATC 8 cut(s) 133, 190, 436, 631, 825, 1042, 1363, 1425
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 1 cut(s) 1470
Bst2UI CCWGG 3 cut(s) 38, 378, 1022
Bst4CI ACNGT 6 cut(s) 130, 206, 681, 688, 1251, 1549
Bst6I CTCTTC 2 cut(s) 396, 408
BstACI GRCGYC 1 cut(s) 5
BstAPI GCANNNNNTGC 1 cut(s) 1244
BstBAI YACGTR 1 cut(s) 983
BstDEI CTNAG 6 cut(s) 306, 723, 886, 1231, 1390, 1418
BstF5I GGATG 4 cut(s) 23, 652, 850, 1231
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 1 cut(s) 7
BstKTI GATC 8 cut(s) 136, 193, 439, 634, 828, 1045, 1366, 1428
BstMAI GTCTC 3 cut(s) 398, 551, 604
BstMBI GATC 8 cut(s) 133, 190, 436, 631, 825, 1042, 1363, 1425
BstMWI GCNNNNNNNGC 2 cut(s) 992, 1244
BstNI CCWGG 3 cut(s) 38, 378, 1022
BstSCI CCNGG 3 cut(s) 36, 376, 1020
BstSFI CTRYAG 4 cut(s) 673, 1116, 1410, 1545
BstSLI GKGCMC 1 cut(s) 1249
BstV1I GCAGC 1 cut(s) 1502
BstV2I GAAGAC 2 cut(s) 69, 686
BstX2I RGATCY 3 cut(s) 133, 436, 1042
BstXI CCANNNNNNTGG 1 cut(s) 1385
BstYI RGATCY 3 cut(s) 133, 436, 1042
BsuRI GGCC 1 cut(s) 222
BtsCI GGATG 4 cut(s) 23, 652, 850, 1231
BtsIMutI CAGTG 4 cut(s) 211, 273, 1247, 1451
CaiI CAGNNNCTG 1 cut(s) 1198
CciI TCATGA 1 cut(s) 1555
CfoI GCGC 1 cut(s) 7
Cfr10I RCCGGY 1 cut(s) 391
Cfr13I GGNCC 2 cut(s) 221, 1564
CseI GACGC 1 cut(s) 80
Csp6I GTAC 6 cut(s) 485, 531, 958, 1148, 1321, 1538
CviQI GTAC 6 cut(s) 485, 531, 958, 1148, 1321, 1538
DdeI CTNAG 6 cut(s) 306, 723, 886, 1231, 1390, 1418
DinI GGCGCC 1 cut(s) 6
DpnI GATC 8 cut(s) 135, 192, 438, 633, 827, 1044, 1365, 1427
DpnII GATC 8 cut(s) 133, 190, 436, 631, 825, 1042, 1363, 1425
DraI TTTAAA 2 cut(s) 1035, 1440
Eam1104I CTCTTC 2 cut(s) 396, 408
EarI CTCTTC 2 cut(s) 396, 408
EciI GGCGGA 1 cut(s) 87
Ecl136II GAGCTC 1 cut(s) 1302
Eco130I CCWWGG 1 cut(s) 1470
Eco24I GRGCYC 2 cut(s) 949, 1304
Eco31I GGTCTC 1 cut(s) 551
Eco47I GGWCC 1 cut(s) 1564
Eco53kI GAGCTC 1 cut(s) 1302
Eco57I CTGAAG 2 cut(s) 1146, 1551
EcoICRI GAGCTC 1 cut(s) 1302
EcoRI GAATTC 1 cut(s) 620
EcoRII CCWGG 3 cut(s) 36, 376, 1020
EcoT14I CCWWGG 1 cut(s) 1470
EcoT38I GRGCYC 2 cut(s) 949, 1304
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 1470
FalI AAGNNNNNCTT 6 cut(s) 869, 901, 1031, 1063, 1350, 1382
FbaI TGATCA 1 cut(s) 190
Fnu4HI GCNGC 1 cut(s) 1516
FokI GGATG 4 cut(s) 10, 659, 857, 1238
FriOI GRGCYC 2 cut(s) 949, 1304
Fsp4HI GCNGC 1 cut(s) 1516
FspBI CTAG 3 cut(s) 1157, 1334, 1404
GlaI GCGC 1 cut(s) 6
GluI GCNGC 1 cut(s) 1516
GsaI CCCAGC 1 cut(s) 884
GsuI CTGGAG 1 cut(s) 1298
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 1 cut(s) 222
HapII CCGG 3 cut(s) 8, 59, 392
HgaI GACGC 1 cut(s) 80
HhaI GCGC 1 cut(s) 7
Hin1I GRCGYC 1 cut(s) 5
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HincII GTYRAC 1 cut(s) 684
HindII GTYRAC 1 cut(s) 684
HinfI GANTC 3 cut(s) 11, 552, 1229
HpaI GTTAAC 1 cut(s) 684
HpaII CCGG 3 cut(s) 8, 59, 392
Hpy166II GTNNAC 4 cut(s) 684, 1247, 1449, 1523
Hpy188III TCNNGA 7 cut(s) 536, 575, 644, 968, 1286, 1370, 1556
Hpy8I GTNNAC 4 cut(s) 684, 1247, 1449, 1523
HpyAV CCTTC 4 cut(s) 99, 347, 701, 1416
HpyCH4III ACNGT 6 cut(s) 130, 206, 681, 688, 1251, 1549
HpyCH4IV ACGT 3 cut(s) 982, 1257, 1323
HpyF10VI GCNNNNNNNGC 2 cut(s) 992, 1244
HpyF3I CTNAG 6 cut(s) 306, 723, 886, 1231, 1390, 1418
HpySE526I ACGT 3 cut(s) 982, 1257, 1323
Hsp92I GRCGYC 1 cut(s) 5
HspAI GCGC 1 cut(s) 5
KasI GGCGCC 1 cut(s) 4
KpnI GGTACC 1 cut(s) 961
Ksp22I TGATCA 1 cut(s) 190
KspAI GTTAAC 1 cut(s) 684
Kzo9I GATC 8 cut(s) 133, 190, 436, 631, 825, 1042, 1363, 1425
LmnI GCTCC 1 cut(s) 100
Lsp1109I GCAGC 1 cut(s) 1502
LweI GCATC 1 cut(s) 640
MaeI CTAG 3 cut(s) 1157, 1334, 1404
MaeII ACGT 3 cut(s) 982, 1257, 1323
MaeIII GTNAC 2 cut(s) 572, 1108
MalI GATC 8 cut(s) 135, 192, 438, 633, 827, 1044, 1365, 1427
MboI GATC 8 cut(s) 133, 190, 436, 631, 825, 1042, 1363, 1425
MflI RGATCY 3 cut(s) 133, 436, 1042
MhlI GDGCHC 5 cut(s) 265, 949, 1071, 1249, 1304
MluCI AATT 7 cut(s) 318, 540, 615, 620, 929, 963, 1099
Mly113I GGCGCC 1 cut(s) 5
MlyI GAGTC 2 cut(s) 5, 1223
MmeI TCCRAC 2 cut(s) 717, 1488
MnlI CCTC 4 cut(s) 298, 718, 1084, 1577
MslI CAYNNNNRTG 1 cut(s) 207
MspA1I CMGCKG 2 cut(s) 1054, 1115
MspI CCGG 3 cut(s) 8, 59, 392
MspR9I CCNGG 3 cut(s) 38, 378, 1022
MssI GTTTAAAC 1 cut(s) 1035
MvaI CCWGG 3 cut(s) 38, 378, 1022
MwoI GCNNNNNNNGC 2 cut(s) 992, 1244
NarI GGCGCC 1 cut(s) 5
NdeII GATC 8 cut(s) 133, 190, 436, 631, 825, 1042, 1363, 1425
NlaIV GGNNCC 3 cut(s) 6, 223, 959
NmuCI GTSAC 1 cut(s) 572
PagI TCATGA 1 cut(s) 1555
PfeI GAWTC 1 cut(s) 552
PkrI GCNGC 1 cut(s) 1517
PleI GAGTC 2 cut(s) 5, 1223
PluTI GGCGCC 1 cut(s) 8
PmeI GTTTAAAC 1 cut(s) 1035
PpsI GAGTC 2 cut(s) 5, 1223
Ppu21I YACGTR 1 cut(s) 983
PshBI ATTAAT 4 cut(s) 321, 614, 899, 932
Psp124BI GAGCTC 1 cut(s) 1304
Psp6I CCWGG 3 cut(s) 36, 376, 1020
PspFI CCCAGC 1 cut(s) 880
PspGI CCWGG 3 cut(s) 36, 376, 1020
PspN4I GGNNCC 3 cut(s) 6, 223, 959
PspPI GGNCC 2 cut(s) 221, 1564
PstI CTGCAG 1 cut(s) 677
PstNI CAGNNNCTG 1 cut(s) 1198
PsuI RGATCY 3 cut(s) 133, 436, 1042
PvuII CAGCTG 2 cut(s) 1054, 1115
RsaI GTAC 6 cut(s) 486, 532, 959, 1149, 1322, 1539
RsaNI GTAC 6 cut(s) 485, 531, 958, 1148, 1321, 1538
RseI CAYNNNNRTG 1 cut(s) 207
SacI GAGCTC 1 cut(s) 1304
SatI GCNGC 1 cut(s) 1516
Sau3AI GATC 8 cut(s) 133, 190, 436, 631, 825, 1042, 1363, 1425
Sau96I GGNCC 2 cut(s) 221, 1564
SchI GAGTC 2 cut(s) 5, 1223
ScrFI CCNGG 3 cut(s) 38, 378, 1022
SduI GDGCHC 5 cut(s) 265, 949, 1071, 1249, 1304
SfaNI GCATC 1 cut(s) 640
SfcI CTRYAG 4 cut(s) 673, 1116, 1410, 1545
SfoI GGCGCC 1 cut(s) 6
SinI GGWCC 1 cut(s) 1564
SmiMI CAYNNNNRTG 1 cut(s) 207
SmlI CTYRAG 1 cut(s) 1070
SmoI CTYRAG 1 cut(s) 1070
Sse9I AATT 7 cut(s) 318, 540, 615, 620, 929, 963, 1099
SsiI CCGC 1 cut(s) 98
SspDI GGCGCC 1 cut(s) 4
SspI AATATT 1 cut(s) 1135
SspMI CTAG 3 cut(s) 1157, 1334, 1404
SstI GAGCTC 1 cut(s) 1304
StyD4I CCNGG 3 cut(s) 36, 376, 1020
StyI CCWWGG 1 cut(s) 1470
TaaI ACNGT 6 cut(s) 130, 206, 681, 688, 1251, 1549
TaiI ACGT 3 cut(s) 985, 1260, 1326
TaqI TCGA 2 cut(s) 831, 1279
TasI AATT 7 cut(s) 318, 540, 615, 620, 929, 963, 1099
TatI WGTACW 1 cut(s) 484
TfiI GAWTC 1 cut(s) 552
TscAI CASTG 4 cut(s) 211, 280, 1254, 1451
TseFI GTSAC 1 cut(s) 572
TseI GCWGC 1 cut(s) 1515
Tsp45I GTSAC 1 cut(s) 572
TspDTI ATGAA 7 cut(s) 351, 378, 602, 613, 890, 1308, 1494
TspGWI ACGGA 2 cut(s) 175, 514
TspRI CASTG 4 cut(s) 211, 280, 1254, 1451
VneI GTGCAC 1 cut(s) 1245
VpaK11BI GGWCC 1 cut(s) 1564
VspI ATTAAT 4 cut(s) 321, 614, 899, 932
XapI RAATTY 2 cut(s) 620, 1099
XspI CTAG 3 cut(s) 1157, 1334, 1404
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.