Rorug05G0296000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000928
Physical Location & Seq
Reverse (-)
34133101 .. 34135745
2645 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0296000.1

Sequence Viewer

Length: 501 bp
ATGAAGGCTACGCGCTTCAGCTCGAGAGGATCAATGTCTGCTACAACGAGGGCCAGTGGCGCTAGGTACGTGCCCATGGACATCGTAAGATCTAAACCGTACATCCAGATCTTCAAGCTGGAAAACTTCATCTTCGACCTGTACACGCCGAGCACAACTGGGCTAAAGAGCGAGGTTCACTGGAATTTGGTGAGGAAGCACTTCAATCTCTTCCAGGGAATATTGGCTCCCAGCTTCGTGGATACAGTCCCAGTCCTTGTTGGTCCAGTGTCCTACTTGTTGCTGTCCAAACCAACAAAGGGTGTTGAAAAGACATTTTCGTTAAAGGAAAACCACCTTGTCGTCTTTACCTCCTGCTCTCTTCTTCACACTGCTGTTGATCTTGTTAATGAGACTAAGCTGGACAATGAAATCAAATTGTGGCTCGCCTTTGCTGCCCAGAAAATTGTTGAAGTTAATGCTTTGGCAAAGGCATTGGCTGGTCACAAGGATGAGGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

166

Amino Acids

18.51

Weight (kDa)

9.49

Isoelectric Point (pI)

21.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 13
AclWI GGATC 1 cut(s) 37
AcsI RAATTY 1 cut(s) 184
AfaI GTAC 3 cut(s) 68, 101, 143
AfiI CCNNNNNNNGG 1 cut(s) 299
AgsI TTSAA 4 cut(s) 115, 205, 308, 452
AjnI CCWGG 1 cut(s) 213
AluBI AGCT 4 cut(s) 21, 118, 234, 400
AluI AGCT 4 cut(s) 21, 118, 234, 400
Alw21I GWGCWC 1 cut(s) 155
Alw26I GTCTC 1 cut(s) 386
AlwI GGATC 1 cut(s) 37
Ama87I CYCGRG 1 cut(s) 22
AoxI GGCC 1 cut(s) 51
ApeKI GCWGC 1 cut(s) 434
ApoI RAATTY 1 cut(s) 184
Asp700I GAANNNNTTC 1 cut(s) 200
AspLEI GCGC 2 cut(s) 15, 62
AspS9I GGNCC 2 cut(s) 51, 263
AsuHPI GGTGA 1 cut(s) 202
AvaI CYCGRG 1 cut(s) 22
AvaII GGWCC 1 cut(s) 263
BaeGI GKGCMC 1 cut(s) 75
Bbv12I GWGCWC 1 cut(s) 155
BbvI GCAGC 1 cut(s) 421
BciT130I CCWGG 1 cut(s) 215
BciVI GTATCC 1 cut(s) 235
BcoDI GTCTC 1 cut(s) 386
BfaI CTAG 1 cut(s) 63
BfoI RGCGCY 1 cut(s) 63
BfuI GTATCC 1 cut(s) 235
BglII AGATCT 2 cut(s) 89, 108
BisI GCNGC 1 cut(s) 435
BlsI GCNGC 1 cut(s) 436
Bme1390I CCNGG 1 cut(s) 215
Bme18I GGWCC 1 cut(s) 263
BmeT110I CYCGRG 1 cut(s) 22
BmgT120I GGNCC 2 cut(s) 51, 263
BmiI GGNNCC 1 cut(s) 228
BmrFI CCNGG 1 cut(s) 215
BmrI ACTGGG 2 cut(s) 168, 245
BmuI ACTGGG 2 cut(s) 168, 245
BsaAI YACGTR 1 cut(s) 70
BsaJI CCNNGG 2 cut(s) 75, 214
Bsc4I CCNNNNNNNGG 1 cut(s) 299
Bse1I ACTGG 5 cut(s) 54, 163, 185, 251, 266
BseBI CCWGG 1 cut(s) 215
BseDI CCNNGG 2 cut(s) 75, 214
BseGI GGATG 2 cut(s) 102, 496
BseLI CCNNNNNNNGG 1 cut(s) 299
BseNI ACTGG 5 cut(s) 54, 163, 185, 251, 266
BseSI GKGCMC 1 cut(s) 75
BseXI GCAGC 1 cut(s) 421
BseYI CCCAGC 1 cut(s) 230
Bsh1236I CGCG 1 cut(s) 13
BshFI GGCC 1 cut(s) 53
BsiHKAI GWGCWC 1 cut(s) 155
BsiHKCI CYCGRG 1 cut(s) 22
BslFI GGGAC 1 cut(s) 233
BslI CCNNNNNNNGG 1 cut(s) 299
BsmAI GTCTC 1 cut(s) 386
BsmFI GGGAC 1 cut(s) 233
BsnI GGCC 1 cut(s) 53
BsoBI CYCGRG 1 cut(s) 22
Bsp1286I GDGCHC 2 cut(s) 75, 155
Bsp1407I TGTACA 1 cut(s) 141
Bsp143I GATC 4 cut(s) 29, 89, 108, 379
Bsp19I CCATGG 1 cut(s) 75
BspANI GGCC 1 cut(s) 53
BspFNI CGCG 1 cut(s) 13
BspLI GGNNCC 1 cut(s) 228
BspPI GGATC 1 cut(s) 37
BsrGI TGTACA 1 cut(s) 141
BsrI ACTGG 5 cut(s) 54, 163, 185, 251, 266
BssECI CCNNGG 2 cut(s) 75, 214
BssMI GATC 4 cut(s) 29, 89, 108, 379
BssT1I CCWWGG 1 cut(s) 75
Bst2UI CCWGG 1 cut(s) 215
Bst4CI ACNGT 2 cut(s) 99, 247
Bst6I CTCTTC 2 cut(s) 215, 366
BstAUI TGTACA 1 cut(s) 141
BstBAI YACGTR 1 cut(s) 70
BstC8I GCNNGC 1 cut(s) 426
BstDEI CTNAG 1 cut(s) 396
BstDSI CCRYGG 1 cut(s) 75
BstF5I GGATG 2 cut(s) 102, 496
BstFNI CGCG 1 cut(s) 13
BstH2I RGCGCY 1 cut(s) 63
BstHHI GCGC 2 cut(s) 15, 62
BstKTI GATC 4 cut(s) 32, 92, 111, 382
BstMAI GTCTC 1 cut(s) 386
BstMBI GATC 4 cut(s) 29, 89, 108, 379
BstMWI GCNNNNNNNGC 2 cut(s) 59, 434
BstNI CCWGG 1 cut(s) 215
BstSCI CCNGG 1 cut(s) 213
BstSLI GKGCMC 1 cut(s) 75
BstUI CGCG 1 cut(s) 13
BstV1I GCAGC 1 cut(s) 421
BstX2I RGATCY 2 cut(s) 89, 108
BstXI CCANNNNNNTGG 1 cut(s) 238
BstYI RGATCY 2 cut(s) 89, 108
BsuI GTATCC 1 cut(s) 235
BsuRI GGCC 1 cut(s) 53
BtgI CCRYGG 1 cut(s) 75
BtsCI GGATG 2 cut(s) 102, 496
BtsI GCAGTG 1 cut(s) 369
BtsIMutI CAGTG 4 cut(s) 61, 178, 273, 369
Cac8I GCNNGC 1 cut(s) 426
CfoI GCGC 2 cut(s) 15, 62
Cfr13I GGNCC 2 cut(s) 51, 263
Csp6I GTAC 3 cut(s) 67, 100, 142
CviAII CATG 1 cut(s) 76
CviQI GTAC 3 cut(s) 67, 100, 142
DdeI CTNAG 1 cut(s) 396
DpnI GATC 4 cut(s) 31, 91, 110, 381
DpnII GATC 4 cut(s) 29, 89, 108, 379
Eam1104I CTCTTC 2 cut(s) 215, 366
EarI CTCTTC 2 cut(s) 215, 366
Eco130I CCWWGG 1 cut(s) 75
Eco47I GGWCC 1 cut(s) 263
Eco88I CYCGRG 1 cut(s) 22
EcoRII CCWGG 1 cut(s) 213
EcoT14I CCWWGG 1 cut(s) 75
ErhI CCWWGG 1 cut(s) 75
FaeI CATG 1 cut(s) 79
FaiI YATR 2 cut(s) 77, 499
FaqI GGGAC 1 cut(s) 233
FatI CATG 1 cut(s) 75
Fnu4HI GCNGC 1 cut(s) 435
FokI GGATG 1 cut(s) 89
Fsp4HI GCNGC 1 cut(s) 435
FspBI CTAG 1 cut(s) 63
GlaI GCGC 2 cut(s) 14, 61
GluI GCNGC 1 cut(s) 435
GsaI CCCAGC 1 cut(s) 234
HaeII RGCGCY 1 cut(s) 63
HaeIII GGCC 1 cut(s) 53
HhaI GCGC 2 cut(s) 15, 62
Hin1II CATG 1 cut(s) 79
Hin6I GCGC 2 cut(s) 13, 60
HinP1I GCGC 2 cut(s) 13, 60
HphI GGTGA 1 cut(s) 202
Hpy166II GTNNAC 2 cut(s) 144, 178
Hpy188III TCNNGA 2 cut(s) 24, 106
Hpy8I GTNNAC 2 cut(s) 144, 178
HpyCH4III ACNGT 2 cut(s) 99, 247
HpyCH4IV ACGT 1 cut(s) 69
HpyF10VI GCNNNNNNNGC 2 cut(s) 59, 434
HpyF3I CTNAG 1 cut(s) 396
HpySE526I ACGT 1 cut(s) 69
Hsp92II CATG 1 cut(s) 79
HspAI GCGC 2 cut(s) 13, 60
Kzo9I GATC 4 cut(s) 29, 89, 108, 379
LmnI GCTCC 1 cut(s) 232
Lsp1109I GCAGC 1 cut(s) 421
MaeI CTAG 1 cut(s) 63
MaeII ACGT 1 cut(s) 69
MaeIII GTNAC 1 cut(s) 482
MalI GATC 4 cut(s) 31, 91, 110, 381
MboI GATC 4 cut(s) 29, 89, 108, 379
MboII GAAGA 5 cut(s) 103, 124, 202, 353, 356
MflI RGATCY 2 cut(s) 89, 108
MhlI GDGCHC 2 cut(s) 75, 155
MluCI AATT 3 cut(s) 184, 416, 444
MnlI CCTC 6 cut(s) 20, 42, 166, 186, 361, 487
MroXI GAANNNNTTC 1 cut(s) 200
MseI TTAA 3 cut(s) 323, 387, 456
MslI CAYNNNNRTG 1 cut(s) 489
MspR9I CCNGG 1 cut(s) 215
MvaI CCWGG 1 cut(s) 215
MvnI CGCG 1 cut(s) 13
MwoI GCNNNNNNNGC 2 cut(s) 59, 434
NcoI CCATGG 1 cut(s) 75
NdeII GATC 4 cut(s) 29, 89, 108, 379
NlaIII CATG 1 cut(s) 79
NlaIV GGNNCC 1 cut(s) 228
NmeAIII GCCGAG 1 cut(s) 174
NmuCI GTSAC 1 cut(s) 482
PaeR7I CTCGAG 1 cut(s) 22
PdmI GAANNNNTTC 1 cut(s) 200
PkrI GCNGC 1 cut(s) 436
Ppu21I YACGTR 1 cut(s) 70
Psp6I CCWGG 1 cut(s) 213
PspFI CCCAGC 1 cut(s) 230
PspGI CCWGG 1 cut(s) 213
PspN4I GGNNCC 1 cut(s) 228
PspPI GGNCC 2 cut(s) 51, 263
PsuI RGATCY 2 cut(s) 89, 108
RsaI GTAC 3 cut(s) 68, 101, 143
RsaNI GTAC 3 cut(s) 67, 100, 142
RseI CAYNNNNRTG 1 cut(s) 489
SaqAI TTAA 3 cut(s) 323, 387, 456
SatI GCNGC 1 cut(s) 435
Sau3AI GATC 4 cut(s) 29, 89, 108, 379
Sau96I GGNCC 2 cut(s) 51, 263
ScrFI CCNGG 1 cut(s) 215
SduI GDGCHC 2 cut(s) 75, 155
Sfr274I CTCGAG 1 cut(s) 22
SinI GGWCC 1 cut(s) 263
SlaI CTCGAG 1 cut(s) 22
SmiMI CAYNNNNRTG 1 cut(s) 489
SmlI CTYRAG 1 cut(s) 22
SmoI CTYRAG 1 cut(s) 22
Sse9I AATT 3 cut(s) 184, 416, 444
SspI AATATT 1 cut(s) 222
SspMI CTAG 1 cut(s) 63
StyD4I CCNGG 1 cut(s) 213
StyI CCWWGG 1 cut(s) 75
TaaI ACNGT 2 cut(s) 99, 247
TaiI ACGT 1 cut(s) 72
TaqI TCGA 2 cut(s) 23, 135
TasI AATT 3 cut(s) 184, 416, 444
TatI WGTACW 1 cut(s) 141
Tru1I TTAA 3 cut(s) 323, 387, 456
Tru9I TTAA 3 cut(s) 323, 387, 456
TscAI CASTG 4 cut(s) 61, 185, 273, 376
TseFI GTSAC 1 cut(s) 482
TseI GCWGC 1 cut(s) 434
Tsp45I GTSAC 1 cut(s) 482
TspDTI ATGAA 3 cut(s) 17, 118, 423
TspRI CASTG 4 cut(s) 61, 185, 273, 376
VpaK11BI GGWCC 1 cut(s) 263
XapI RAATTY 1 cut(s) 184
XhoI CTCGAG 1 cut(s) 22
XmnI GAANNNNTTC 1 cut(s) 200
XspI CTAG 1 cut(s) 63
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.