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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 228 |
| AciI | CCGC | 1 cut(s) | 147 |
| AcsI | RAATTY | 1 cut(s) | 222 |
| AcuI | CTGAAG | 2 cut(s) | 201, 254 |
| AfaI | GTAC | 1 cut(s) | 178 |
| Alw21I | GWGCWC | 1 cut(s) | 205 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 194 |
| ApeKI | GCWGC | 2 cut(s) | 11, 171 |
| ApoI | RAATTY | 1 cut(s) | 222 |
| AsuII | TTCGAA | 1 cut(s) | 142 |
| BauI | CACGAG | 1 cut(s) | 108 |
| Bbv12I | GWGCWC | 1 cut(s) | 205 |
| BbvI | GCAGC | 1 cut(s) | 158 |
| BccI | CCATC | 1 cut(s) | 67 |
| BfaI | CTAG | 1 cut(s) | 319 |
| BfmI | CTRYAG | 3 cut(s) | 90, 161, 172 |
| BisI | GCNGC | 2 cut(s) | 12, 172 |
| BlsI | GCNGC | 2 cut(s) | 13, 173 |
| BmcAI | AGTACT | 1 cut(s) | 178 |
| BmsI | GCATC | 2 cut(s) | 80, 175 |
| Bpu14I | TTCGAA | 1 cut(s) | 142 |
| BseGI | GGATG | 2 cut(s) | 59, 71 |
| BseMII | CTCAG | 1 cut(s) | 141 |
| BseXI | GCAGC | 1 cut(s) | 158 |
| BsiHKAI | GWGCWC | 1 cut(s) | 205 |
| Bsp119I | TTCGAA | 1 cut(s) | 142 |
| Bsp1286I | GDGCHC | 1 cut(s) | 205 |
| BspACI | CCGC | 1 cut(s) | 147 |
| BspCNI | CTCAG | 1 cut(s) | 140 |
| BspMAI | CTGCAG | 1 cut(s) | 176 |
| BspT104I | TTCGAA | 1 cut(s) | 142 |
| BssSI | CACGAG | 1 cut(s) | 108 |
| Bst2BI | CACGAG | 1 cut(s) | 108 |
| BstBI | TTCGAA | 1 cut(s) | 142 |
| BstDEI | CTNAG | 1 cut(s) | 127 |
| BstF5I | GGATG | 2 cut(s) | 59, 71 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 200 |
| BstSFI | CTRYAG | 3 cut(s) | 90, 161, 172 |
| BstV1I | GCAGC | 1 cut(s) | 158 |
| BtsCI | GGATG | 2 cut(s) | 59, 71 |
| CaiI | CAGNNNCTG | 1 cut(s) | 194 |
| CseI | GACGC | 1 cut(s) | 17 |
| Csp6I | GTAC | 1 cut(s) | 177 |
| CviAII | CATG | 1 cut(s) | 68 |
| CviJI | RGCY | 4 cut(s) | 126, 137, 171, 194 |
| CviKI_1 | RGCY | 4 cut(s) | 126, 137, 171, 194 |
| CviQI | GTAC | 1 cut(s) | 177 |
| DdeI | CTNAG | 1 cut(s) | 127 |
| Eco57I | CTGAAG | 2 cut(s) | 201, 254 |
| EcoT22I | ATGCAT | 1 cut(s) | 73 |
| FaeI | CATG | 1 cut(s) | 71 |
| FaiI | YATR | 6 cut(s) | 41, 69, 87, 104, 228, 290 |
| FatI | CATG | 1 cut(s) | 67 |
| Fnu4HI | GCNGC | 2 cut(s) | 12, 172 |
| FokI | GGATG | 2 cut(s) | 46, 58 |
| Fsp4HI | GCNGC | 2 cut(s) | 12, 172 |
| FspBI | CTAG | 1 cut(s) | 319 |
| GluI | GCNGC | 2 cut(s) | 12, 172 |
| HgaI | GACGC | 1 cut(s) | 17 |
| Hin1II | CATG | 1 cut(s) | 71 |
| HinfI | GANTC | 1 cut(s) | 211 |
| HpyAV | CCTTC | 3 cut(s) | 116, 161, 229 |
| HpyCH4IV | ACGT | 1 cut(s) | 263 |
| HpyCH4V | TGCA | 4 cut(s) | 71, 174, 188, 292 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 200 |
| HpyF3I | CTNAG | 1 cut(s) | 127 |
| HpySE526I | ACGT | 1 cut(s) | 263 |
| Hsp92II | CATG | 1 cut(s) | 71 |
| LmnI | GCTCC | 1 cut(s) | 208 |
| LpnPI | CCDG | 1 cut(s) | 149 |
| Lsp1109I | GCAGC | 1 cut(s) | 158 |
| LweI | GCATC | 2 cut(s) | 80, 175 |
| MaeI | CTAG | 1 cut(s) | 319 |
| MaeII | ACGT | 1 cut(s) | 263 |
| MboII | GAAGA | 2 cut(s) | 194, 260 |
| MhlI | GDGCHC | 1 cut(s) | 205 |
| MluCI | AATT | 2 cut(s) | 222, 283 |
| MnlI | CCTC | 4 cut(s) | 85, 104, 127, 184 |
| Mph1103I | ATGCAT | 1 cut(s) | 73 |
| MseI | TTAA | 1 cut(s) | 152 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 200 |
| NlaIII | CATG | 1 cut(s) | 71 |
| NsiI | ATGCAT | 1 cut(s) | 73 |
| NspV | TTCGAA | 1 cut(s) | 142 |
| PfeI | GAWTC | 1 cut(s) | 211 |
| PkrI | GCNGC | 2 cut(s) | 13, 173 |
| PsiI | TTATAA | 1 cut(s) | 228 |
| PstI | CTGCAG | 1 cut(s) | 176 |
| PstNI | CAGNNNCTG | 1 cut(s) | 194 |
| RsaI | GTAC | 1 cut(s) | 178 |
| RsaNI | GTAC | 1 cut(s) | 177 |
| SaqAI | TTAA | 1 cut(s) | 152 |
| SatI | GCNGC | 2 cut(s) | 12, 172 |
| ScaI | AGTACT | 1 cut(s) | 178 |
| SduI | GDGCHC | 1 cut(s) | 205 |
| SetI | ASST | 3 cut(s) | 120, 240, 266 |
| SfaNI | GCATC | 2 cut(s) | 80, 175 |
| SfcI | CTRYAG | 3 cut(s) | 90, 161, 172 |
| SfuI | TTCGAA | 1 cut(s) | 142 |
| SgeI | CNNG | 6 cut(s) | 80, 120, 122, 176, 220, 316 |
| Sse9I | AATT | 2 cut(s) | 222, 283 |
| SsiI | CCGC | 1 cut(s) | 147 |
| SspMI | CTAG | 1 cut(s) | 319 |
| TaiI | ACGT | 1 cut(s) | 266 |
| TaqI | TCGA | 1 cut(s) | 142 |
| TasI | AATT | 2 cut(s) | 222, 283 |
| TatI | WGTACW | 1 cut(s) | 176 |
| TfiI | GAWTC | 1 cut(s) | 211 |
| Tru1I | TTAA | 1 cut(s) | 152 |
| Tru9I | TTAA | 1 cut(s) | 152 |
| TseI | GCWGC | 2 cut(s) | 11, 171 |
| TspDTI | ATGAA | 1 cut(s) | 17 |
| XapI | RAATTY | 1 cut(s) | 222 |
| XspI | CTAG | 1 cut(s) | 319 |
| ZrmI | AGTACT | 1 cut(s) | 178 |
| Zsp2I | ATGCAT | 1 cut(s) | 73 |