Rmu_sc0004834.1_g000012

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004834.1
OG0000928
Physical Location & Seq
Reverse (-)
32491 .. 38782
6292 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004834.1_g000012.1.cds

Sequence Viewer

Length: 1578 bp
atggcgccggactcaaaatcagcacccctacagcaccaggaagaagaagaaccacaaccggaagacctcgtcacactttcacacaacccttctgctccgcctgatgagttattcgacatctcaacgacagtagatccgagttatgtgatctctctgatacggaaacttctaccagctaatgccagtaatcatcacaactctcgcggtgaggtttcatgtggccctgtgcaaggattaaatgttgatgacatggaaaaaagtgctctaactcgctctggtgttccgcccccatcaacggacacatctgagagcatggaaattaatgacgattttaatgaaaatgctacccacgaaggagaaagtgaggcggaacagcctcgtcatagtgtgccggtgggagaagaggcttgggaagagtatggttgcatattatgggatctttctgcaagtaaaactcatgcagaactcatggtgcagaaccttgtacttgaagtgctgttagcaaaccttatggtttcacaatccgttcgtactatggaaattggtctcggaatcatcggaaaccttgcctgtcacgaagttcccatgaaacatatagtctctactaatggattaactgaattcattgtggatcagatgtttctggatgatgctcaatgcttatgtgaagtctgcagactgttaactgtgggtcttcaaagtagtgaaggtgttacttgggctgaggcattgcagtcagaacagtatctgacccacattttatggattgcagaaaatagcttgaacacacagcttatagaaaagagtgctgaactattgttagcgatcatcgaaagttcgcaggatgttgcgcatattctcttaccgccattgatgaagctgggcttagcaagtctattaataaatctcttggatattgaaacaagcaaattaatgagtgaaagagctcctgaaaggtacccaattcttgatgtagttcttcatgccattgaagctctttctgttattgatggtcattcccaggacatctgtttagacaaggatctctttcagctggtttgtgccttgctcaagttccctgataaagttgaggttgcaaactcttgtgttacagctgtagttcttgttgcaaatattttttcagacgtacctactctagcttcagagttatcaaatgatatgcttttcttacagggtctgcttgatgtgtttccttttactgcggatgactcagaagcaaggagtgcactgtggaacatcattgcaaggttactggttcgagttccagaaaatgaaatgagctcatcaactctccagaagtacgttttggttctagtttgcaaatctgatgtgattgaagatgatcttcttgactgccagttaggtggcttaggattaaaggctagaactacagctctcagaaggatcatcagtattttaaaccagtggactgcttcaaaagattatgccaaggagaatgaaaataatataaacatcgatagattgttggattgctgccgcaaacattctgaagatgtaccatctacagtttctcatgacatggacctcaaagaatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

525

Amino Acids

57.89

Weight (kDa)

4.44

Isoelectric Point (pI)

58.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 852
Acc65I GGTACC 1 cut(s) 957
AccB1I GGYRCC 2 cut(s) 4, 957
AccII CGCG 1 cut(s) 204
AciI CCGC 7 cut(s) 98, 204, 284, 368, 866, 1223, 1519
AclWI GGATC 5 cut(s) 128, 444, 639, 1050, 1433
AcsI RAATTY 1 cut(s) 620
AcuI CTGAAG 2 cut(s) 1146, 1551
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 6 cut(s) 486, 532, 959, 1149, 1322, 1539
AfiI CCNNNNNNNGG 2 cut(s) 230, 295
AgsI TTSAA 7 cut(s) 491, 698, 784, 920, 992, 1358, 1458
AjnI CCWGG 2 cut(s) 36, 1020
AjuI GAANNNNNNNTTGG 2 cut(s) 1310, 1342
Alw21I GWGCWC 4 cut(s) 265, 949, 1249, 1304
Alw26I GTCTC 2 cut(s) 551, 604
Alw44I GTGCAC 1 cut(s) 1245
AlwI GGATC 5 cut(s) 128, 444, 639, 1050, 1433
AlwNI CAGNNNCTG 2 cut(s) 748, 1198
AoxI GGCC 1 cut(s) 220
ApaLI GTGCAC 1 cut(s) 1245
ApeKI GCWGC 1 cut(s) 1515
ApoI RAATTY 1 cut(s) 620
AseI ATTAAT 3 cut(s) 321, 899, 932
Asp718I GGTACC 1 cut(s) 957
AspLEI GCGC 2 cut(s) 7, 853
AspS9I GGNCC 2 cut(s) 221, 1564
AsuHPI GGTGA 1 cut(s) 218
AvaII GGWCC 1 cut(s) 1564
BaeGI GKGCMC 1 cut(s) 1249
BanI GGYRCC 2 cut(s) 4, 957
BanII GRGCYC 2 cut(s) 949, 1304
BbsI GAAGAC 2 cut(s) 69, 686
Bbv12I GWGCWC 4 cut(s) 265, 949, 1249, 1304
BbvCI CCTCAGC 1 cut(s) 723
BbvI GCAGC 1 cut(s) 1502
BccI CCATC 3 cut(s) 298, 1004, 1549
BciT130I CCWGG 2 cut(s) 38, 1022
BcoDI GTCTC 2 cut(s) 551, 604
BfaI CTAG 3 cut(s) 1157, 1334, 1404
BfmI CTRYAG 5 cut(s) 29, 673, 1116, 1410, 1545
BfoI RGCGCY 1 cut(s) 8
BisI GCNGC 2 cut(s) 1516, 1519
BlpI GCTNAGC 1 cut(s) 886
BlsI GCNGC 2 cut(s) 1517, 1520
Bme1390I CCNGG 2 cut(s) 38, 1022
Bme18I GGWCC 1 cut(s) 1564
BmgT120I GGNCC 2 cut(s) 221, 1564
BmiI GGNNCC 2 cut(s) 6, 959
BmrFI CCNGG 2 cut(s) 38, 1022
BmsI GCATC 1 cut(s) 640
BpiI GAAGAC 2 cut(s) 69, 686
BpmI CTGGAG 1 cut(s) 1298
Bpu10I CCTNAGC 2 cut(s) 723, 1390
Bpu1102I GCTNAGC 1 cut(s) 886
BpuEI CTTGAG 1 cut(s) 1055
Bsa29I ATCGAT 1 cut(s) 1497
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 551
BsaJI CCNNGG 2 cut(s) 1020, 1470
BsaWI WCCGGW 1 cut(s) 58
Bsc4I CCNNNNNNNGG 2 cut(s) 230, 295
Bse118I RCCGGY 1 cut(s) 391
Bse1I ACTGG 4 cut(s) 183, 1278, 1378, 1444
Bse3DI GCAATG 2 cut(s) 728, 1260
BseBI CCWGG 2 cut(s) 38, 1022
BseCI ATCGAT 1 cut(s) 1497
BseDI CCNNGG 2 cut(s) 1020, 1470
BseGI GGATG 3 cut(s) 652, 850, 1231
BseLI CCNNNNNNNGG 2 cut(s) 230, 295
BseMI GCAATG 2 cut(s) 728, 1260
BseMII CTCAG 4 cut(s) 297, 714, 1245, 1432
BseNI ACTGG 4 cut(s) 183, 1278, 1378, 1444
BseSI GKGCMC 1 cut(s) 1249
BseXI GCAGC 1 cut(s) 1502
BseYI CCCAGC 1 cut(s) 880
BsgI GTGCAG 1 cut(s) 494
Bsh1236I CGCG 1 cut(s) 204
BshFI GGCC 1 cut(s) 222
BshNI GGYRCC 2 cut(s) 4, 957
BshVI ATCGAT 1 cut(s) 1497
BsiHKAI GWGCWC 4 cut(s) 265, 949, 1249, 1304
BsiSI CCGG 3 cut(s) 8, 59, 392
BslI CCNNNNNNNGG 2 cut(s) 230, 295
BsmAI GTCTC 2 cut(s) 551, 604
BsnI GGCC 1 cut(s) 222
Bso31I GGTCTC 1 cut(s) 551
Bsp1286I GDGCHC 4 cut(s) 265, 949, 1249, 1304
Bsp143I GATC 8 cut(s) 133, 147, 436, 631, 825, 1042, 1363, 1425
Bsp1720I GCTNAGC 1 cut(s) 886
BspACI CCGC 7 cut(s) 98, 204, 284, 368, 866, 1223, 1519
BspANI GGCC 1 cut(s) 222
BspCNI CTCAG 4 cut(s) 298, 715, 1244, 1431
BspDI ATCGAT 1 cut(s) 1497
BspFNI CGCG 1 cut(s) 204
BspHI TCATGA 1 cut(s) 1555
BspLI GGNNCC 2 cut(s) 6, 959
BspMAI CTGCAG 1 cut(s) 677
BspPI GGATC 5 cut(s) 128, 444, 639, 1050, 1433
BspT107I GGYRCC 2 cut(s) 4, 957
BspTNI GGTCTC 1 cut(s) 551
BsrDI GCAATG 2 cut(s) 728, 1260
BsrFI RCCGGY 1 cut(s) 391
BsrI ACTGG 4 cut(s) 183, 1278, 1378, 1444
BssAI RCCGGY 1 cut(s) 391
BssECI CCNNGG 2 cut(s) 1020, 1470
BssMI GATC 8 cut(s) 133, 147, 436, 631, 825, 1042, 1363, 1425
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 1 cut(s) 1470
Bst2UI CCWGG 2 cut(s) 38, 1022
Bst4CI ACNGT 6 cut(s) 130, 681, 688, 744, 1251, 1549
Bst6I CTCTTC 2 cut(s) 396, 408
BstACI GRCGYC 1 cut(s) 5
BstAPI GCANNNNNTGC 1 cut(s) 1244
BstDEI CTNAG 6 cut(s) 306, 723, 886, 1231, 1390, 1418
BstENI CCTNNNNNAGG 1 cut(s) 228
BstF5I GGATG 3 cut(s) 652, 850, 1231
BstFNI CGCG 1 cut(s) 204
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 2 cut(s) 7, 853
BstKTI GATC 8 cut(s) 136, 150, 439, 634, 828, 1045, 1366, 1428
BstMAI GTCTC 2 cut(s) 551, 604
BstMBI GATC 8 cut(s) 133, 147, 436, 631, 825, 1042, 1363, 1425
BstMWI GCNNNNNNNGC 2 cut(s) 992, 1244
BstNI CCWGG 2 cut(s) 38, 1022
BstSCI CCNGG 2 cut(s) 36, 1020
BstSFI CTRYAG 5 cut(s) 29, 673, 1116, 1410, 1545
BstSLI GKGCMC 1 cut(s) 1249
BstUI CGCG 1 cut(s) 204
BstV1I GCAGC 1 cut(s) 1502
BstV2I GAAGAC 2 cut(s) 69, 686
BstX2I RGATCY 3 cut(s) 133, 436, 1042
BstXI CCANNNNNNTGG 1 cut(s) 1385
BstYI RGATCY 3 cut(s) 133, 436, 1042
Bsu15I ATCGAT 1 cut(s) 1497
BsuRI GGCC 1 cut(s) 222
BsuTUI ATCGAT 1 cut(s) 1497
BtsCI GGATG 3 cut(s) 652, 850, 1231
BtsIMutI CAGTG 2 cut(s) 1247, 1451
CaiI CAGNNNCTG 2 cut(s) 748, 1198
CciI TCATGA 1 cut(s) 1555
CfoI GCGC 2 cut(s) 7, 853
Cfr10I RCCGGY 1 cut(s) 391
Cfr13I GGNCC 2 cut(s) 221, 1564
ClaI ATCGAT 1 cut(s) 1497
Csp6I GTAC 6 cut(s) 485, 531, 958, 1148, 1321, 1538
CviAII CATG 9 cut(s) 216, 250, 313, 458, 469, 586, 983, 1556, 1561
CviQI GTAC 6 cut(s) 485, 531, 958, 1148, 1321, 1538
DdeI CTNAG 6 cut(s) 306, 723, 886, 1231, 1390, 1418
DinI GGCGCC 1 cut(s) 6
DpnI GATC 8 cut(s) 135, 149, 438, 633, 827, 1044, 1365, 1427
DpnII GATC 8 cut(s) 133, 147, 436, 631, 825, 1042, 1363, 1425
DraI TTTAAA 1 cut(s) 1440
Eam1104I CTCTTC 2 cut(s) 396, 408
EarI CTCTTC 2 cut(s) 396, 408
EciI GGCGGA 3 cut(s) 87, 273, 383
Ecl136II GAGCTC 2 cut(s) 947, 1302
Eco130I CCWWGG 1 cut(s) 1470
Eco24I GRGCYC 2 cut(s) 949, 1304
Eco31I GGTCTC 1 cut(s) 551
Eco47I GGWCC 1 cut(s) 1564
Eco53kI GAGCTC 2 cut(s) 947, 1302
Eco57I CTGAAG 2 cut(s) 1146, 1551
EcoICRI GAGCTC 2 cut(s) 947, 1302
EcoNI CCTNNNNNAGG 1 cut(s) 228
EcoRI GAATTC 1 cut(s) 620
EcoRII CCWGG 2 cut(s) 36, 1020
EcoT14I CCWWGG 1 cut(s) 1470
EcoT38I GRGCYC 2 cut(s) 949, 1304
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 1470
FaeI CATG 9 cut(s) 219, 253, 316, 461, 472, 589, 986, 1559, 1564
FalI AAGNNNNNCTT 6 cut(s) 869, 901, 1031, 1063, 1350, 1382
FatI CATG 9 cut(s) 215, 249, 312, 457, 468, 585, 982, 1555, 1560
Fnu4HI GCNGC 2 cut(s) 1516, 1519
FokI GGATG 3 cut(s) 659, 857, 1238
FriOI GRGCYC 2 cut(s) 949, 1304
Fsp4HI GCNGC 2 cut(s) 1516, 1519
FspBI CTAG 3 cut(s) 1157, 1334, 1404
FspI TGCGCA 1 cut(s) 852
GlaI GCGC 2 cut(s) 6, 852
GluI GCNGC 2 cut(s) 1516, 1519
GsaI CCCAGC 1 cut(s) 884
GsuI CTGGAG 1 cut(s) 1298
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 1 cut(s) 222
HapII CCGG 3 cut(s) 8, 59, 392
HhaI GCGC 2 cut(s) 7, 853
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 9 cut(s) 219, 253, 316, 461, 472, 589, 986, 1559, 1564
Hin6I GCGC 2 cut(s) 5, 851
HinP1I GCGC 2 cut(s) 5, 851
HincII GTYRAC 1 cut(s) 684
HindII GTYRAC 1 cut(s) 684
HinfI GANTC 3 cut(s) 11, 552, 1229
HpaI GTTAAC 1 cut(s) 684
HpaII CCGG 3 cut(s) 8, 59, 392
HphI GGTGA 1 cut(s) 218
Hpy166II GTNNAC 3 cut(s) 684, 1247, 1449
Hpy188III TCNNGA 8 cut(s) 575, 644, 950, 968, 1286, 1315, 1370, 1556
Hpy8I GTNNAC 3 cut(s) 684, 1247, 1449
HpyAV CCTTC 4 cut(s) 99, 347, 701, 1416
HpyCH4III ACNGT 6 cut(s) 130, 681, 688, 744, 1251, 1549
HpyCH4IV ACGT 2 cut(s) 1146, 1323
HpyF10VI GCNNNNNNNGC 2 cut(s) 992, 1244
HpyF3I CTNAG 6 cut(s) 306, 723, 886, 1231, 1390, 1418
HpySE526I ACGT 2 cut(s) 1146, 1323
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 9 cut(s) 219, 253, 316, 461, 472, 589, 986, 1559, 1564
HspAI GCGC 2 cut(s) 5, 851
KasI GGCGCC 1 cut(s) 4
KpnI GGTACC 1 cut(s) 961
KspAI GTTAAC 1 cut(s) 684
Kzo9I GATC 8 cut(s) 133, 147, 436, 631, 825, 1042, 1363, 1425
LmnI GCTCC 2 cut(s) 100, 952
Lsp1109I GCAGC 1 cut(s) 1502
LweI GCATC 1 cut(s) 640
MaeI CTAG 3 cut(s) 1157, 1334, 1404
MaeII ACGT 2 cut(s) 1146, 1323
MaeIII GTNAC 5 cut(s) 70, 572, 712, 1108, 1269
MalI GATC 8 cut(s) 135, 149, 438, 633, 827, 1044, 1365, 1427
MboI GATC 8 cut(s) 133, 147, 436, 631, 825, 1042, 1363, 1425
MflI RGATCY 3 cut(s) 133, 436, 1042
MhlI GDGCHC 4 cut(s) 265, 949, 1249, 1304
MluCI AATT 5 cut(s) 318, 540, 620, 929, 963
Mly113I GGCGCC 1 cut(s) 5
MlyI GAGTC 2 cut(s) 5, 1223
MmeI TCCRAC 1 cut(s) 1488
MnlI CCTC 8 cut(s) 77, 202, 358, 387, 397, 718, 1084, 1577
MseI TTAA 9 cut(s) 236, 321, 333, 614, 683, 899, 932, 1397, 1439
MspA1I CMGCKG 2 cut(s) 1054, 1115
MspI CCGG 3 cut(s) 8, 59, 392
MspR9I CCNGG 2 cut(s) 38, 1022
MvaI CCWGG 2 cut(s) 38, 1022
MvnI CGCG 1 cut(s) 204
MwoI GCNNNNNNNGC 2 cut(s) 992, 1244
NarI GGCGCC 1 cut(s) 5
NdeII GATC 8 cut(s) 133, 147, 436, 631, 825, 1042, 1363, 1425
NlaIII CATG 9 cut(s) 219, 253, 316, 461, 472, 589, 986, 1559, 1564
NlaIV GGNNCC 2 cut(s) 6, 959
NmuCI GTSAC 2 cut(s) 70, 572
NsbI TGCGCA 1 cut(s) 852
PagI TCATGA 1 cut(s) 1555
PfeI GAWTC 1 cut(s) 552
PflFI GACNNNGTC 1 cut(s) 68
PkrI GCNGC 2 cut(s) 1517, 1520
PleI GAGTC 2 cut(s) 5, 1223
PluTI GGCGCC 1 cut(s) 8
PpsI GAGTC 2 cut(s) 5, 1223
PshBI ATTAAT 3 cut(s) 321, 899, 932
Psp124BI GAGCTC 2 cut(s) 949, 1304
Psp6I CCWGG 2 cut(s) 36, 1020
PspFI CCCAGC 1 cut(s) 880
PspGI CCWGG 2 cut(s) 36, 1020
PspN4I GGNNCC 2 cut(s) 6, 959
PspPI GGNCC 2 cut(s) 221, 1564
PstI CTGCAG 1 cut(s) 677
PstNI CAGNNNCTG 2 cut(s) 748, 1198
PsuI RGATCY 3 cut(s) 133, 436, 1042
PsyI GACNNNGTC 1 cut(s) 68
PvuII CAGCTG 2 cut(s) 1054, 1115
RsaI GTAC 6 cut(s) 486, 532, 959, 1149, 1322, 1539
RsaNI GTAC 6 cut(s) 485, 531, 958, 1148, 1321, 1538
SacI GAGCTC 2 cut(s) 949, 1304
SaqAI TTAA 9 cut(s) 236, 321, 333, 614, 683, 899, 932, 1397, 1439
SatI GCNGC 2 cut(s) 1516, 1519
Sau3AI GATC 8 cut(s) 133, 147, 436, 631, 825, 1042, 1363, 1425
Sau96I GGNCC 2 cut(s) 221, 1564
SchI GAGTC 2 cut(s) 5, 1223
ScrFI CCNGG 2 cut(s) 38, 1022
SduI GDGCHC 4 cut(s) 265, 949, 1249, 1304
SfaNI GCATC 1 cut(s) 640
SfcI CTRYAG 5 cut(s) 29, 673, 1116, 1410, 1545
SfoI GGCGCC 1 cut(s) 6
SinI GGWCC 1 cut(s) 1564
SmlI CTYRAG 1 cut(s) 1070
SmoI CTYRAG 1 cut(s) 1070
Sse9I AATT 5 cut(s) 318, 540, 620, 929, 963
SsiI CCGC 7 cut(s) 98, 204, 284, 368, 866, 1223, 1519
SspDI GGCGCC 1 cut(s) 4
SspI AATATT 1 cut(s) 1135
SspMI CTAG 3 cut(s) 1157, 1334, 1404
SstI GAGCTC 2 cut(s) 949, 1304
StyD4I CCNGG 2 cut(s) 36, 1020
StyI CCWWGG 1 cut(s) 1470
TaaI ACNGT 6 cut(s) 130, 681, 688, 744, 1251, 1549
TaiI ACGT 2 cut(s) 1149, 1326
TaqI TCGA 4 cut(s) 114, 831, 1279, 1497
TasI AATT 5 cut(s) 318, 540, 620, 929, 963
TatI WGTACW 1 cut(s) 484
TauI GCSGC 1 cut(s) 1521
TfiI GAWTC 1 cut(s) 552
Tru1I TTAA 9 cut(s) 236, 321, 333, 614, 683, 899, 932, 1397, 1439
Tru9I TTAA 9 cut(s) 236, 321, 333, 614, 683, 899, 932, 1397, 1439
TscAI CASTG 2 cut(s) 1254, 1451
TseFI GTSAC 2 cut(s) 70, 572
TseI GCWGC 1 cut(s) 1515
Tsp45I GTSAC 2 cut(s) 70, 572
TspDTI ATGAA 8 cut(s) 204, 351, 602, 613, 890, 971, 1308, 1494
TspGWI ACGGA 3 cut(s) 175, 311, 514
TspRI CASTG 2 cut(s) 1254, 1451
Tth111I GACNNNGTC 1 cut(s) 68
VneI GTGCAC 1 cut(s) 1245
VpaK11BI GGWCC 1 cut(s) 1564
VspI ATTAAT 3 cut(s) 321, 899, 932
XagI CCTNNNNNAGG 1 cut(s) 228
XapI RAATTY 1 cut(s) 620
XspI CTAG 3 cut(s) 1157, 1334, 1404
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.