AT5G23870

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0007825
Physical Location & Seq
Reverse (-)
8045540 .. 8050395
4856 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G23870.4

Sequence Viewer

Length: 1248 bp
ATGAAGACGACGACTCGGCTCCTCGATCTAACAGCGGCTATGGTTCTAGTTGTCTACGTGTCTTTCTCTCCTCCGCTTGTTTCCGGCGAACCAGGGCGGCGTGTTAGCATGACACTGGTTCGAGACGCCGCTGCTCTCGGTGCTTTTTGCTTGGACGGAAGCTTACCGGCGTATCATTTAGACAGAGGGTTTGGTGCTGGATCAAACAATTGGATTTTGCAGTTTGAGGGAGGAGGATGGTGCAATGATATAGCATCATGTGTGGAAAGAGCCAAGACCCGTCGAGGCTCGACACGTTATATGAGCAAGACCGTCGTCTTCACTGGAGTCTTGAGCAATAACGCCTCTCAAAATCCAGATTTTTACAACTGGAACAAAGTCAGGCTAAGGTATTGCGACGGAGCTTCATTTGCGGGAGATTCACAATTTGGCAACGGGACGTCATTGCTTTATTTCAGAGGACAACGAATATGGAATGCCATTATTCTTGACCTTCTCCCCAAAGGTTTAGCAAAAGCCCACAAGGCTCTTCTAACCGGTTGTTCAGCTGGTGGTTTGTCTACATTTTTGCATTGTGACAATTTCACGAGCTATTTACCCAAAAACGCAAGCGTTAAGTGCATGAGCGATGCTGGATTCTTTCTTGACGCAATCGATGTAGCAGCAAACCGGACAATGAGATCTTTTTACTCACAGCTTGTGTCTCTACAGGGTATACAAAAAAACCTCGATCCAAGTTGCACACACGCATTTTTCCCCGAACCATCTCTGTGTTTTTTCCCGCAATACGTATTGCGATTTATCAAAACGCCATTTTTCATCTTAAATTCAGCCTACGATGTATTTCAGTTCCATCATGGACTGGTTCCACCTTCTGCTGATCAAACCGGACGTTGGAACCGTTGTAAGCTTAATGTGACGGCATGTAATCCACACCAACTCGATGCACTACAAGGGTTTAGGAAAGATATGTTGGGAGCTTTAATGAATTTCTTCAGAAATTCTACGAGAGGAGGAATGTTTATAAACTCATGCTTTGATCATTGTCAAAGTGCTTTAGAGGAGACTTGGCTCTCTCCCACATCACCAAGAATCAACAATAAGACAATTGCTGAGACGGTCGGAGATTGGTATTTTGGGAGAGGAGAAGAAGCGAAAGAAATTGGTTGTCCATATCCATGTGATAAAACATGTCATAATCTCATTCCAGCTTCTACTTCAGATTTTTTGGTAAATCTAGATATTTAA

Protein Analysis

415

Amino Acids

45.96

Weight (kDa)

8.54

Isoelectric Point (pI)

45.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 33 - 399 1.7e-161 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1025
AatII GACGTC 1 cut(s) 443
AccI GTMKAC 3 cut(s) 54, 560, 715
AciI CCGC 6 cut(s) 35, 74, 97, 129, 413, 782
AclWI GGATC 2 cut(s) 208, 725
AcsI RAATTY 3 cut(s) 826, 988, 1000
AcuI CTGAAG 2 cut(s) 979, 1203
AcyI GRCGYC 2 cut(s) 126, 440
AfiI CCNNNNNNNGG 1 cut(s) 894
AflIII ACRYGT 3 cut(s) 57, 293, 1190
AgeI ACCGGT 1 cut(s) 536
AjnI CCWGG 1 cut(s) 91
AjuI GAANNNNNNNTTGG 2 cut(s) 956, 988
AluBI AGCT 8 cut(s) 162, 404, 548, 591, 697, 910, 980, 1211
AluI AGCT 8 cut(s) 162, 404, 548, 591, 697, 910, 980, 1211
Alw26I GTCTC 4 cut(s) 117, 708, 1058, 1109
AlwI GGATC 2 cut(s) 208, 725
ApeKI GCWGC 2 cut(s) 131, 662
ApoI RAATTY 3 cut(s) 826, 988, 1000
ArsI GACNNNNNNTTYG 2 cut(s) 173, 205
AsiGI ACCGGT 1 cut(s) 536
Asp700I GAANNNNTTC 1 cut(s) 992
AsuHPI GGTGA 1 cut(s) 1077
BaeI ACNNNNGTAYC 2 cut(s) 155, 188
BauI CACGAG 1 cut(s) 586
BbsI GAAGAC 2 cut(s) 11, 310
BbvI GCAGC 2 cut(s) 118, 674
BccI CCATC 3 cut(s) 231, 772, 861
BceAI ACGGC 1 cut(s) 936
BcgI CGANNNNNNTGC 2 cut(s) 295, 329
BciT130I CCWGG 1 cut(s) 93
BclI TGATCA 2 cut(s) 880, 1039
BcoDI GTCTC 4 cut(s) 117, 708, 1058, 1109
BfaI CTAG 2 cut(s) 47, 1238
BfmI CTRYAG 1 cut(s) 707
BglI GCCNNNNNGGC 1 cut(s) 524
BglII AGATCT 1 cut(s) 680
BisI GCNGC 5 cut(s) 36, 98, 129, 132, 663
BlsI GCNGC 5 cut(s) 37, 99, 130, 133, 664
Bme1390I CCNGG 1 cut(s) 93
BmiI GGNNCC 3 cut(s) 20, 867, 899
BmrFI CCNGG 1 cut(s) 93
BmsI GCATC 3 cut(s) 263, 619, 934
BoxI GACNNNNGTC 1 cut(s) 314
BpiI GAAGAC 2 cut(s) 11, 310
BpmI CTGGAG 1 cut(s) 345
Bpu10I CCTNAGC 1 cut(s) 386
BpuEI CTTGAG 1 cut(s) 352
Bsa29I ATCGAT 1 cut(s) 654
BsaAI YACGTR 2 cut(s) 58, 790
BsaHI GRCGYC 2 cut(s) 126, 440
BsaJI CCNNGG 1 cut(s) 92
BsaWI WCCGGW 3 cut(s) 536, 669, 887
Bsc4I CCNNNNNNNGG 1 cut(s) 894
Bse118I RCCGGY 2 cut(s) 166, 536
Bse1I ACTGG 4 cut(s) 120, 328, 374, 867
Bse3DI GCAATG 2 cut(s) 250, 443
BseBI CCWGG 1 cut(s) 93
BseCI ATCGAT 1 cut(s) 654
BseDI CCNNGG 1 cut(s) 92
BseGI GGATG 1 cut(s) 242
BseLI CCNNNNNNNGG 1 cut(s) 894
BseMI GCAATG 2 cut(s) 250, 443
BseMII CTCAG 1 cut(s) 1104
BseNI ACTGG 4 cut(s) 120, 328, 374, 867
BseRI GAGGAG 6 cut(s) 11, 60, 246, 1026, 1076, 1158
BseXI GCAGC 2 cut(s) 118, 674
Bsh1285I CGRYCG 1 cut(s) 1122
BshTI ACCGGT 1 cut(s) 536
BshVI ATCGAT 1 cut(s) 654
BsiEI CGRYCG 1 cut(s) 1122
BsiSI CCGG 5 cut(s) 84, 167, 537, 670, 888
BslFI GGGAC 1 cut(s) 451
BslI CCNNNNNNNGG 1 cut(s) 894
BsmAI GTCTC 4 cut(s) 117, 708, 1058, 1109
BsmBI CGTCTC 2 cut(s) 117, 1109
BsmFI GGGAC 1 cut(s) 451
BsmI GAATGC 1 cut(s) 481
Bsp143I GATC 6 cut(s) 25, 200, 680, 730, 880, 1039
BspACI CCGC 6 cut(s) 35, 74, 97, 129, 413, 782
BspCNI CTCAG 1 cut(s) 1105
BspDI ATCGAT 1 cut(s) 654
BspLI GGNNCC 3 cut(s) 20, 867, 899
BspPI GGATC 2 cut(s) 208, 725
BspQI GCTCTTC 1 cut(s) 534
BsrDI GCAATG 2 cut(s) 250, 443
BsrFI RCCGGY 2 cut(s) 166, 536
BsrI ACTGG 4 cut(s) 120, 328, 374, 867
BssAI RCCGGY 2 cut(s) 166, 536
BssECI CCNNGG 1 cut(s) 92
BssMI GATC 6 cut(s) 25, 200, 680, 730, 880, 1039
BssNAI GTATAC 1 cut(s) 716
BssNI GRCGYC 2 cut(s) 126, 440
BssSI CACGAG 1 cut(s) 586
Bst1107I GTATAC 1 cut(s) 716
Bst2BI CACGAG 1 cut(s) 586
Bst2UI CCWGG 1 cut(s) 93
Bst4CI ACNGT 3 cut(s) 313, 902, 1120
Bst6I CTCTTC 1 cut(s) 534
BstACI GRCGYC 2 cut(s) 126, 440
BstBAI YACGTR 2 cut(s) 58, 790
BstC8I GCNNGC 1 cut(s) 610
BstDEI CTNAG 2 cut(s) 386, 1113
BstF5I GGATG 1 cut(s) 242
BstKTI GATC 6 cut(s) 28, 203, 683, 733, 883, 1042
BstMAI GTCTC 4 cut(s) 117, 708, 1058, 1109
BstMBI GATC 6 cut(s) 25, 200, 680, 730, 880, 1039
BstMCI CGRYCG 1 cut(s) 1122
BstMWI GCNNNNNNNGC 4 cut(s) 140, 410, 524, 618
BstNI CCWGG 1 cut(s) 93
BstNSI RCATGY 2 cut(s) 927, 1194
BstPAI GACNNNNGTC 1 cut(s) 314
BstSCI CCNGG 1 cut(s) 91
BstSFI CTRYAG 1 cut(s) 707
BstSNI TACGTA 1 cut(s) 790
BstV1I GCAGC 2 cut(s) 118, 674
BstV2I GAAGAC 2 cut(s) 11, 310
BstX2I RGATCY 1 cut(s) 680
BstYI RGATCY 1 cut(s) 680
BstZ17I GTATAC 1 cut(s) 716
Bsu15I ATCGAT 1 cut(s) 654
BsuTUI ATCGAT 1 cut(s) 654
BtgZI GCGATG 1 cut(s) 642
BtsCI GGATG 1 cut(s) 242
BtsIMutI CAGTG 2 cut(s) 113, 321
Cac8I GCNNGC 1 cut(s) 610
Cfr10I RCCGGY 2 cut(s) 166, 536
ClaI ATCGAT 1 cut(s) 654
CseI GACGC 2 cut(s) 134, 656
CspAI ACCGGT 1 cut(s) 536
CviAII CATG 8 cut(s) 109, 258, 622, 857, 924, 1032, 1179, 1191
DdeI CTNAG 2 cut(s) 386, 1113
DpnI GATC 6 cut(s) 27, 202, 682, 732, 882, 1041
DpnII GATC 6 cut(s) 25, 200, 680, 730, 880, 1039
Eam1104I CTCTTC 1 cut(s) 534
EarI CTCTTC 1 cut(s) 534
Eco105I TACGTA 1 cut(s) 790
Eco57I CTGAAG 2 cut(s) 979, 1203
EcoRII CCWGG 1 cut(s) 91
Esp3I CGTCTC 2 cut(s) 117, 1109
FaeI CATG 8 cut(s) 112, 261, 625, 860, 927, 1035, 1182, 1194
FaqI GGGAC 1 cut(s) 451
FatI CATG 8 cut(s) 108, 257, 621, 856, 923, 1031, 1178, 1190
FauI CCCGC 2 cut(s) 406, 789
FbaI TGATCA 2 cut(s) 880, 1039
FblI GTMKAC 3 cut(s) 54, 560, 715
Fnu4HI GCNGC 5 cut(s) 36, 98, 129, 132, 663
FokI GGATG 1 cut(s) 249
Fsp4HI GCNGC 5 cut(s) 36, 98, 129, 132, 663
FspBI CTAG 2 cut(s) 47, 1238
GluI GCNGC 5 cut(s) 36, 98, 129, 132, 663
GsuI CTGGAG 1 cut(s) 345
HapII CCGG 5 cut(s) 84, 167, 537, 670, 888
HgaI GACGC 2 cut(s) 134, 656
Hin1I GRCGYC 2 cut(s) 126, 440
Hin1II CATG 8 cut(s) 112, 261, 625, 860, 927, 1035, 1182, 1194
HindIII AAGCTT 2 cut(s) 160, 908
HinfI GANTC 5 cut(s) 13, 327, 419, 636, 1092
HpaII CCGG 5 cut(s) 84, 167, 537, 670, 888
HphI GGTGA 1 cut(s) 1077
Hpy166II GTNNAC 3 cut(s) 55, 561, 716
Hpy188I TCNGA 4 cut(s) 458, 998, 1124, 1222
Hpy188III TCNNGA 7 cut(s) 122, 331, 356, 488, 586, 644, 1238
Hpy8I GTNNAC 3 cut(s) 55, 561, 716
Hpy99I CGWCG 4 cut(s) 13, 285, 317, 401
HpyAV CCTTC 2 cut(s) 503, 882
HpyCH4III ACNGT 3 cut(s) 313, 902, 1120
HpyCH4IV ACGT 5 cut(s) 57, 295, 440, 789, 892
HpyCH4V TGCA 6 cut(s) 220, 243, 571, 621, 741, 947
HpyF10VI GCNNNNNNNGC 4 cut(s) 140, 410, 524, 618
HpyF3I CTNAG 2 cut(s) 386, 1113
HpySE526I ACGT 5 cut(s) 57, 295, 440, 789, 892
Hsp92I GRCGYC 2 cut(s) 126, 440
Hsp92II CATG 8 cut(s) 112, 261, 625, 860, 927, 1035, 1182, 1194
Ksp22I TGATCA 2 cut(s) 880, 1039
Kzo9I GATC 6 cut(s) 25, 200, 680, 730, 880, 1039
LguI GCTCTTC 1 cut(s) 534
LmnI GCTCC 3 cut(s) 24, 401, 977
Lsp1109I GCAGC 2 cut(s) 118, 674
LweI GCATC 3 cut(s) 263, 619, 934
MaeI CTAG 2 cut(s) 47, 1238
MaeII ACGT 5 cut(s) 57, 295, 440, 789, 892
MaeIII GTNAC 2 cut(s) 575, 916
MalI GATC 6 cut(s) 27, 202, 682, 732, 882, 1041
MboI GATC 6 cut(s) 25, 200, 680, 730, 880, 1039
MboII GAAGA 5 cut(s) 16, 310, 521, 985, 1160
MfeI CAATTG 2 cut(s) 208, 1107
MflI RGATCY 1 cut(s) 680
MluCI AATT 8 cut(s) 208, 425, 580, 826, 988, 1000, 1107, 1161
MlyI GAGTC 2 cut(s) 7, 336
MmeI TCCRAC 2 cut(s) 875, 1102
MroXI GAANNNNTTC 1 cut(s) 992
MseI TTAA 5 cut(s) 615, 824, 912, 983, 1246
MslI CAYNNNNRTG 2 cut(s) 769, 1177
MspA1I CMGCKG 3 cut(s) 35, 131, 548
MspI CCGG 5 cut(s) 84, 167, 537, 670, 888
MspR9I CCNGG 1 cut(s) 93
MunI CAATTG 2 cut(s) 208, 1107
Mva1269I GAATGC 1 cut(s) 481
MvaI CCWGG 1 cut(s) 93
MwoI GCNNNNNNNGC 4 cut(s) 140, 410, 524, 618
NdeII GATC 6 cut(s) 25, 200, 680, 730, 880, 1039
NlaIII CATG 8 cut(s) 112, 261, 625, 860, 927, 1035, 1182, 1194
NlaIV GGNNCC 3 cut(s) 20, 867, 899
NmuCI GTSAC 2 cut(s) 575, 916
NspI RCATGY 2 cut(s) 927, 1194
PciI ACATGT 1 cut(s) 1190
PciSI GCTCTTC 1 cut(s) 534
PcsI WCGNNNNNNNCGW 1 cut(s) 898
PctI GAATGC 1 cut(s) 481
PdmI GAANNNNTTC 1 cut(s) 992
PfeI GAWTC 3 cut(s) 419, 636, 1092
PinAI ACCGGT 1 cut(s) 536
PkrI GCNGC 5 cut(s) 37, 99, 130, 133, 664
PleI GAGTC 2 cut(s) 7, 335
PpsI GAGTC 2 cut(s) 7, 335
Ppu21I YACGTR 2 cut(s) 58, 790
PscI ACATGT 1 cut(s) 1190
PshAI GACNNNNGTC 1 cut(s) 314
PsiI TTATAA 1 cut(s) 1025
Psp6I CCWGG 1 cut(s) 91
PspGI CCWGG 1 cut(s) 91
PspN4I GGNNCC 3 cut(s) 20, 867, 899
PsuI RGATCY 1 cut(s) 680
PvuII CAGCTG 1 cut(s) 548
RseI CAYNNNNRTG 2 cut(s) 769, 1177
SapI GCTCTTC 1 cut(s) 534
SaqAI TTAA 5 cut(s) 615, 824, 912, 983, 1246
SatI GCNGC 5 cut(s) 36, 98, 129, 132, 663
Sau3AI GATC 6 cut(s) 25, 200, 680, 730, 880, 1039
SchI GAGTC 2 cut(s) 7, 336
ScrFI CCNGG 1 cut(s) 93
SfaNI GCATC 3 cut(s) 263, 619, 934
SfcI CTRYAG 1 cut(s) 707
SmiMI CAYNNNNRTG 2 cut(s) 769, 1177
SmlI CTYRAG 1 cut(s) 331
SmoI CTYRAG 1 cut(s) 331
SnaBI TACGTA 1 cut(s) 790
Sse9I AATT 8 cut(s) 208, 425, 580, 826, 988, 1000, 1107, 1161
SsiI CCGC 6 cut(s) 35, 74, 97, 129, 413, 782
SspMI CTAG 2 cut(s) 47, 1238
StyD4I CCNGG 1 cut(s) 91
TaaI ACNGT 3 cut(s) 313, 902, 1120
TaiI ACGT 5 cut(s) 60, 298, 443, 792, 895
TaqI TCGA 7 cut(s) 24, 121, 283, 290, 654, 729, 942
TasI AATT 8 cut(s) 208, 425, 580, 826, 988, 1000, 1107, 1161
TauI GCSGC 3 cut(s) 38, 100, 131
TfiI GAWTC 3 cut(s) 419, 636, 1092
Tru1I TTAA 5 cut(s) 615, 824, 912, 983, 1246
Tru9I TTAA 5 cut(s) 615, 824, 912, 983, 1246
TscAI CASTG 2 cut(s) 120, 328
TseFI GTSAC 2 cut(s) 575, 916
TseI GCWGC 2 cut(s) 131, 662
Tsp45I GTSAC 2 cut(s) 575, 916
TspDTI ATGAA 4 cut(s) 17, 396, 808, 1001
TspGWI ACGGA 2 cut(s) 171, 414
TspRI CASTG 2 cut(s) 120, 328
XapI RAATTY 3 cut(s) 826, 988, 1000
XbaI TCTAGA 1 cut(s) 1237
XceI RCATGY 2 cut(s) 927, 1194
XmiI GTMKAC 3 cut(s) 54, 560, 715
XmnI GAANNNNTTC 1 cut(s) 992
XspI CTAG 2 cut(s) 47, 1238
ZraI GACGTC 1 cut(s) 441
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.