Prupe.2G296000_v2.0.a1

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0007825
Physical Location & Seq
Reverse (-)
28689833 .. 28694255
4423 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G296000.1

Sequence Viewer

Length: 1248 bp
ATGTTAAAATTGAAGAGAGAGCGCATGGCTACTACGACGACGAATGCGAACGCGCTACTGGTCGCCATCATCCTGGCGCTGCTAATGTGCTCGCCTCGGTGCATTTACTCGGCCGAGCGGCGGCTCCTGGTCAACATGACTCTGATCCGGAACGCTCCGGCTCTCGGAGCTTTTTGCTTGGATGGGAGTTTGCCTGCGTATCACCTGCACAGAGGATTCGGGGCCGGAGCAAGAAACTGGCTTTTACAGTTCGAGGGTGGTGGGTGGTGCAATGACATAGAATCATGCTTGGAGAGAGCCAAAAGCCGTAGAGGATCAACACGCTACATGACCAAGTGGGAGGTCTTCTCTGGAATTCTAAGCAATAATGCATCTCTAAATCCAGATTTTTACAACTGGAACCGTGTGAAGCTTAGATATTGCGATGGAGCTTCATTTGCTGGAGATGCCGTATTTAAGAATGGGACATCGTTGCTCTATTTCAGAGGGCAAAAGATTTGGGAAGCTATCATTCTTGATCTTCTGCCAAAAGGTTTAGGACAAGCAAGAAAGGCTCTGTTATCAGGATGTTCTGCTGGGGGTTTAGCATCCTTTTTGCATTGTAACAACTTCACTAAATATTTACCAAGCAATGCCAGTGTGAAATGCTTGAGTGATGCTGGATTCTTTCTTGATGAAAGAGATATAACTTCGAATCACACCATGAGGTATTTCATTAAAGATGTTGTTTCTCTACAGTATGCATGGCAGGGGGTAGAGAAGAATCTGGATGAGAACTGCACCGCCAGCTCCCTTGATTTTCCCGAGTTGTGTTTCTTCCCACAGTACGCACTGAAGTTCATTACAACACCATTTTTCATATTGAACTCAGCTTATGATGTTTATCAGTTCCATCATATATTGGTGCCATCTTCAGCTGATCCACACGGCCACTGGAACCGTTGCAAACTGAATCCGGCAGCGTGCAATCCTGAACAGTTAAATACATTGCAAGAATTCAGGCGTGACATGATTGTTGCTATGGGATTGTTCTACAAATATTCGAGAAGAGGAGGCTTGTTCATAAATTCATGCTTTGCTCACTGCCAAAGTGAGTCTCAAGACACATGGTTTTCTGCAGATTCTCCAAGAGTTCACGATAAGACCATTGCAGAAGCAGTAGGTGATTGGTACTTCAGCAGAAGGATAACAAAGGAAATTGACTGCCCATATCCTTGTGACACTACCTGCCATAACCTTATACCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04310 map04310
Pfam Domains
Protein Families
PAE

Protein Analysis

416

Amino Acids

46.93

Weight (kDa)

8.57

Isoelectric Point (pI)

45.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 213
Acc36I ACCTGC 2 cut(s) 213, 1235
AccB1I GGYRCC 1 cut(s) 904
AccBSI CCGCTC 1 cut(s) 118
AccII CGCG 1 cut(s) 53
AccIII TCCGGA 1 cut(s) 147
AciI CCGC 3 cut(s) 118, 121, 783
AclWI GGATC 3 cut(s) 139, 322, 914
AcoI YGGCCR 2 cut(s) 111, 928
AcsI RAATTY 3 cut(s) 354, 995, 1066
AcuI CTGAAG 3 cut(s) 854, 897, 1159
AfaI GTAC 2 cut(s) 827, 1172
AfiI CCNNNNNNNGG 2 cut(s) 120, 164
AgsI TTSAA 2 cut(s) 13, 865
AjnI CCWGG 2 cut(s) 72, 126
AluBI AGCT 7 cut(s) 170, 412, 431, 506, 789, 872, 917
AluI AGCT 7 cut(s) 170, 412, 431, 506, 789, 872, 917
Alw21I GWGCWC 1 cut(s) 92
Alw26I GTCTC 1 cut(s) 1101
AlwI GGATC 3 cut(s) 139, 322, 914
Ama87I CYCGRG 1 cut(s) 803
Aor13HI TCCGGA 1 cut(s) 147
AoxI GGCC 3 cut(s) 111, 222, 928
ApeKI GCWGC 2 cut(s) 79, 959
ApoI RAATTY 3 cut(s) 354, 995, 1066
AspLEI GCGC 3 cut(s) 24, 55, 79
AspS9I GGNCC 1 cut(s) 222
AsuHPI GGTGA 2 cut(s) 194, 1175
AsuII TTCGAA 1 cut(s) 692
AvaI CYCGRG 1 cut(s) 803
BanI GGYRCC 1 cut(s) 904
BbsI GAAGAC 1 cut(s) 337
Bbv12I GWGCWC 1 cut(s) 92
BbvI GCAGC 2 cut(s) 66, 971
BccI CCATC 5 cut(s) 74, 176, 419, 900, 916
BceAI ACGGC 3 cut(s) 291, 434, 943
BciT130I CCWGG 2 cut(s) 74, 128
BcoDI GTCTC 1 cut(s) 1101
BfmI CTRYAG 2 cut(s) 734, 1116
BfoI RGCGCY 1 cut(s) 80
BfuAI ACCTGC 2 cut(s) 213, 1235
BisI GCNGC 4 cut(s) 80, 119, 122, 960
BlsI GCNGC 4 cut(s) 81, 120, 123, 961
Bme1390I CCNGG 2 cut(s) 74, 128
BmeT110I CYCGRG 1 cut(s) 803
BmgT120I GGNCC 1 cut(s) 222
BmiI GGNNCC 5 cut(s) 125, 223, 401, 906, 938
BmrFI CCNGG 2 cut(s) 74, 128
BmsI GCATC 4 cut(s) 380, 436, 596, 646
BpiI GAAGAC 1 cut(s) 337
BplI GAGNNNNNCTC 2 cut(s) 332, 364
BpmI CTGGAG 1 cut(s) 462
Bpu14I TTCGAA 1 cut(s) 692
BpuEI CTTGAG 2 cut(s) 670, 1083
BsaBI GATNNNNATC 1 cut(s) 882
BsaJI CCNNGG 1 cut(s) 95
BsaWI WCCGGW 1 cut(s) 147
BsaXI ACNNNNNCTCC 2 cut(s) 435, 465
Bsc4I CCNNNNNNNGG 2 cut(s) 120, 164
Bse1I ACTGG 5 cut(s) 63, 242, 401, 636, 938
Bse3DI GCAATG 4 cut(s) 277, 637, 986, 1146
Bse8I GATNNNNATC 1 cut(s) 882
BseAI TCCGGA 1 cut(s) 147
BseBI CCWGG 2 cut(s) 74, 128
BseDI CCNNGG 1 cut(s) 95
BseGI GGATG 5 cut(s) 69, 187, 572, 587, 775
BseJI GATNNNNATC 1 cut(s) 882
BseLI CCNNNNNNNGG 2 cut(s) 120, 164
BseMI GCAATG 4 cut(s) 277, 637, 986, 1146
BseMII CTCAG 1 cut(s) 882
BseNI ACTGG 5 cut(s) 63, 242, 401, 636, 938
BseRI GAGGAG 1 cut(s) 1065
BseX3I CGGCCG 1 cut(s) 111
BseXI GCAGC 2 cut(s) 66, 971
BseYI CCCAGC 1 cut(s) 575
BsgI GTGCAG 2 cut(s) 191, 763
Bsh1236I CGCG 1 cut(s) 53
Bsh1285I CGRYCG 1 cut(s) 114
BshFI GGCC 3 cut(s) 113, 224, 930
BshNI GGYRCC 1 cut(s) 904
BsiEI CGRYCG 1 cut(s) 114
BsiHKAI GWGCWC 1 cut(s) 92
BsiHKCI CYCGRG 1 cut(s) 803
BsiSI CCGG 4 cut(s) 148, 158, 225, 956
BslFI GGGAC 1 cut(s) 478
BslI CCNNNNNNNGG 2 cut(s) 120, 164
BsmAI GTCTC 1 cut(s) 1101
BsmFI GGGAC 1 cut(s) 478
BsmI GAATGC 1 cut(s) 49
BsnI GGCC 3 cut(s) 113, 224, 930
BsoBI CYCGRG 1 cut(s) 803
Bsp119I TTCGAA 1 cut(s) 692
Bsp1286I GDGCHC 1 cut(s) 92
Bsp13I TCCGGA 1 cut(s) 147
Bsp143I GATC 4 cut(s) 144, 314, 517, 919
BspACI CCGC 3 cut(s) 118, 121, 783
BspANI GGCC 3 cut(s) 113, 224, 930
BspCNI CTCAG 1 cut(s) 881
BspEI TCCGGA 1 cut(s) 147
BspFNI CGCG 1 cut(s) 53
BspLI GGNNCC 5 cut(s) 125, 223, 401, 906, 938
BspMAI CTGCAG 1 cut(s) 1120
BspMI ACCTGC 2 cut(s) 213, 1235
BspPI GGATC 3 cut(s) 139, 322, 914
BspT104I TTCGAA 1 cut(s) 692
BspT107I GGYRCC 1 cut(s) 904
BsrBI CCGCTC 1 cut(s) 118
BsrDI GCAATG 4 cut(s) 277, 637, 986, 1146
BsrI ACTGG 5 cut(s) 63, 242, 401, 636, 938
BssECI CCNNGG 1 cut(s) 95
BssMI GATC 4 cut(s) 144, 314, 517, 919
Bst2UI CCWGG 2 cut(s) 74, 128
Bst4CI ACNGT 6 cut(s) 249, 404, 738, 825, 941, 978
Bst6I CTCTTC 2 cut(s) 8, 1042
BstBI TTCGAA 1 cut(s) 692
BstC8I GCNNGC 4 cut(s) 92, 195, 787, 964
BstDEI CTNAG 3 cut(s) 359, 413, 868
BstF5I GGATG 5 cut(s) 69, 187, 572, 587, 775
BstFNI CGCG 1 cut(s) 53
BstH2I RGCGCY 1 cut(s) 80
BstHHI GCGC 3 cut(s) 24, 55, 79
BstKTI GATC 4 cut(s) 147, 317, 520, 922
BstMAI GTCTC 1 cut(s) 1101
BstMBI GATC 4 cut(s) 144, 314, 517, 919
BstMCI CGRYCG 1 cut(s) 114
BstMWI GCNNNNNNNGC 5 cut(s) 167, 437, 446, 551, 786
BstNI CCWGG 2 cut(s) 74, 128
BstSCI CCNGG 2 cut(s) 72, 126
BstSFI CTRYAG 2 cut(s) 734, 1116
BstUI CGCG 1 cut(s) 53
BstV1I GCAGC 2 cut(s) 66, 971
BstV2I GAAGAC 1 cut(s) 337
BstXI CCANNNNNNTGG 1 cut(s) 73
BstZI CGGCCG 1 cut(s) 111
BsuRI GGCC 3 cut(s) 113, 224, 930
BtgZI GCGATG 1 cut(s) 438
BtsCI GGATG 5 cut(s) 69, 187, 572, 587, 775
BtsI GCAGTG 1 cut(s) 1081
BtsIMutI CAGTG 4 cut(s) 643, 830, 931, 1081
BveI ACCTGC 2 cut(s) 213, 1235
Cac8I GCNNGC 4 cut(s) 92, 195, 787, 964
CfoI GCGC 3 cut(s) 24, 55, 79
Cfr13I GGNCC 1 cut(s) 222
Csp6I GTAC 2 cut(s) 826, 1171
CviAII CATG 9 cut(s) 25, 136, 285, 328, 703, 744, 1009, 1071, 1107
CviQI GTAC 2 cut(s) 826, 1171
DdeI CTNAG 3 cut(s) 359, 413, 868
DpnI GATC 4 cut(s) 146, 316, 519, 921
DpnII GATC 4 cut(s) 144, 314, 517, 919
EaeI YGGCCR 2 cut(s) 111, 928
EagI CGGCCG 1 cut(s) 111
Eam1104I CTCTTC 2 cut(s) 8, 1042
EarI CTCTTC 2 cut(s) 8, 1042
EclXI CGGCCG 1 cut(s) 111
Eco52I CGGCCG 1 cut(s) 111
Eco57I CTGAAG 3 cut(s) 854, 897, 1159
Eco88I CYCGRG 1 cut(s) 803
EcoRI GAATTC 2 cut(s) 354, 995
EcoRII CCWGG 2 cut(s) 72, 126
EcoT22I ATGCAT 2 cut(s) 373, 745
FaeI CATG 9 cut(s) 28, 139, 288, 331, 706, 747, 1012, 1074, 1110
FaqI GGGAC 1 cut(s) 478
FatI CATG 9 cut(s) 24, 135, 284, 327, 702, 743, 1008, 1070, 1106
Fnu4HI GCNGC 4 cut(s) 80, 119, 122, 960
FokI GGATG 5 cut(s) 56, 194, 574, 579, 782
Fsp4HI GCNGC 4 cut(s) 80, 119, 122, 960
GlaI GCGC 3 cut(s) 23, 54, 78
GluI GCNGC 4 cut(s) 80, 119, 122, 960
GsaI CCCAGC 1 cut(s) 579
GsuI CTGGAG 1 cut(s) 462
HaeII RGCGCY 1 cut(s) 80
HaeIII GGCC 3 cut(s) 113, 224, 930
HapII CCGG 4 cut(s) 148, 158, 225, 956
HhaI GCGC 3 cut(s) 24, 55, 79
Hin1II CATG 9 cut(s) 28, 139, 288, 331, 706, 747, 1012, 1074, 1110
Hin6I GCGC 3 cut(s) 22, 53, 77
HinP1I GCGC 3 cut(s) 22, 53, 77
HincII GTYRAC 1 cut(s) 133
HindII GTYRAC 1 cut(s) 133
HindIII AAGCTT 1 cut(s) 410
HinfI GANTC 9 cut(s) 139, 216, 281, 663, 694, 763, 952, 1094, 1121
HpaII CCGG 4 cut(s) 148, 158, 225, 956
HphI GGTGA 2 cut(s) 194, 1175
Hpy166II GTNNAC 2 cut(s) 133, 1135
Hpy188I TCNGA 3 cut(s) 144, 167, 485
Hpy8I GTNNAC 2 cut(s) 133, 1135
Hpy99I CGWCG 2 cut(s) 40, 43
HpyAV CCTTC 1 cut(s) 1176
HpyCH4III ACNGT 6 cut(s) 249, 404, 738, 825, 941, 978
HpyF10VI GCNNNNNNNGC 5 cut(s) 167, 437, 446, 551, 786
HpyF3I CTNAG 3 cut(s) 359, 413, 868
Hsp92II CATG 9 cut(s) 28, 139, 288, 331, 706, 747, 1012, 1074, 1110
HspAI GCGC 3 cut(s) 22, 53, 77
Kpn2I TCCGGA 1 cut(s) 147
Kzo9I GATC 4 cut(s) 144, 314, 517, 919
LmnI GCTCC 6 cut(s) 129, 160, 167, 227, 428, 794
Lsp1109I GCAGC 2 cut(s) 66, 971
LweI GCATC 4 cut(s) 380, 436, 596, 646
MaeIII GTNAC 3 cut(s) 602, 1004, 1217
MalI GATC 4 cut(s) 146, 316, 519, 921
MbiI CCGCTC 1 cut(s) 118
MboI GATC 4 cut(s) 144, 314, 517, 919
MboII GAAGA 7 cut(s) 25, 337, 512, 772, 808, 903, 1059
MhlI GDGCHC 1 cut(s) 92
MluCI AATT 5 cut(s) 8, 354, 995, 1066, 1197
MlyI GAGTC 2 cut(s) 133, 1103
MnlI CCTC 9 cut(s) 105, 206, 247, 305, 334, 479, 699, 1043, 1046
Mph1103I ATGCAT 2 cut(s) 373, 745
MroI TCCGGA 1 cut(s) 147
MseI TTAA 4 cut(s) 5, 456, 717, 980
MspA1I CMGCKG 1 cut(s) 917
MspI CCGG 4 cut(s) 148, 158, 225, 956
MspR9I CCNGG 2 cut(s) 74, 128
Mva1269I GAATGC 1 cut(s) 49
MvaI CCWGG 2 cut(s) 74, 128
MvnI CGCG 1 cut(s) 53
MwoI GCNNNNNNNGC 5 cut(s) 167, 437, 446, 551, 786
NdeII GATC 4 cut(s) 144, 314, 517, 919
NlaIII CATG 9 cut(s) 28, 139, 288, 331, 706, 747, 1012, 1074, 1110
NlaIV GGNNCC 5 cut(s) 125, 223, 401, 906, 938
NmeAIII GCCGAG 2 cut(s) 89, 139
NmuCI GTSAC 2 cut(s) 1004, 1217
NsiI ATGCAT 2 cut(s) 373, 745
NspV TTCGAA 1 cut(s) 692
PaqCI CACCTGC 1 cut(s) 213
PcsI WCGNNNNNNNCGW 1 cut(s) 44
PctI GAATGC 1 cut(s) 49
PfeI GAWTC 7 cut(s) 216, 281, 663, 694, 763, 952, 1121
PkrI GCNGC 4 cut(s) 81, 120, 123, 961
PleI GAGTC 2 cut(s) 133, 1102
PpsI GAGTC 2 cut(s) 133, 1102
Psp6I CCWGG 2 cut(s) 72, 126
PspFI CCCAGC 1 cut(s) 575
PspGI CCWGG 2 cut(s) 72, 126
PspN4I GGNNCC 5 cut(s) 125, 223, 401, 906, 938
PspPI GGNCC 1 cut(s) 222
PstI CTGCAG 1 cut(s) 1120
PvuII CAGCTG 1 cut(s) 917
RsaI GTAC 2 cut(s) 827, 1172
RsaNI GTAC 2 cut(s) 826, 1171
SaqAI TTAA 4 cut(s) 5, 456, 717, 980
SatI GCNGC 4 cut(s) 80, 119, 122, 960
Sau3AI GATC 4 cut(s) 144, 314, 517, 919
Sau96I GGNCC 1 cut(s) 222
SchI GAGTC 2 cut(s) 133, 1103
ScrFI CCNGG 2 cut(s) 74, 128
SduI GDGCHC 1 cut(s) 92
SfaNI GCATC 4 cut(s) 380, 436, 596, 646
SfcI CTRYAG 2 cut(s) 734, 1116
SfuI TTCGAA 1 cut(s) 692
SmlI CTYRAG 2 cut(s) 649, 1098
SmoI CTYRAG 2 cut(s) 649, 1098
Sse9I AATT 5 cut(s) 8, 354, 995, 1066, 1197
SsiI CCGC 3 cut(s) 118, 121, 783
SspI AATATT 2 cut(s) 620, 1040
StyD4I CCNGG 2 cut(s) 72, 126
TaaI ACNGT 6 cut(s) 249, 404, 738, 825, 941, 978
TaqI TCGA 3 cut(s) 252, 692, 1043
TasI AATT 5 cut(s) 8, 354, 995, 1066, 1197
TauI GCSGC 2 cut(s) 121, 124
TfiI GAWTC 7 cut(s) 216, 281, 663, 694, 763, 952, 1121
Tru1I TTAA 4 cut(s) 5, 456, 717, 980
Tru9I TTAA 4 cut(s) 5, 456, 717, 980
TscAI CASTG 4 cut(s) 643, 837, 938, 1088
TseFI GTSAC 2 cut(s) 1004, 1217
TseI GCWGC 2 cut(s) 79, 959
Tsp45I GTSAC 2 cut(s) 1004, 1217
TspDTI ATGAA 7 cut(s) 423, 690, 703, 829, 847, 1051, 1059
TspRI CASTG 4 cut(s) 643, 837, 938, 1088
XapI RAATTY 3 cut(s) 354, 995, 1066
XcmI CCANNNNNNNNNTGG 1 cut(s) 930
Zsp2I ATGCAT 2 cut(s) 373, 745
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.