MD07G1272700.v1.1

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0007825
Physical Location & Seq
Reverse (-)
33895441 .. 33901986
6546 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1272700.v1.1.491

Sequence Viewer

Length: 1227 bp
ATGATGTATGTCAATGCAGTCCTGGTCGCCGTAACGACAGCTCTGTTGGCGTGCGCTCCTCGGTGCATTTACGCCGAGGAGCGGCTCACGGTGAACATGACCATTGTCCGACGCGGTTCAGCTCTCGGAGCTTTTTGCTTGGATGGGAGTTTGCCTGCGTATCATCTGCACAGAGGATTCGGGGCCGGAGCAAGAAACTGGCTTTTGCAGTTTGAGGGTGGAGGGTGGTGCAATGATGTAAAATCATGCTCTGATAGAGCCAGCACGCGAAGAGGATCAACACGTTATATGACCAAGTGGGAGGTCTTTTCTGGAATCCTCAGCAATAATGCATCTCTTAATCCAGATTTTTACAACTGGAACCGAGTGAAGCTTAGATATTGTGATGGAGCTTCATTTGCTGGAGATGCAGTAGTTAATAACGGGACATCGTTGCTCTATTTCAGAGGGCAAAAGATTTGGGAAGCAATCATCCTTGATCTTCTGCCGAAAGGTTTACGACAAGCACGTAAGGCTTTGCTATCAGGTTGTTCTGCTGGGGGTTTAGCATCGTTTTTGCACTGTAACAGCTTCACTAACTACTTACCAAGCAATACCAGTGTGAAATGCTTGAGTGACGCCGGATTCTTTCTCGATGAACGAGATATAAATTTGAACCACACCATGAGGTATTTCGTAAAAGATCTTGTGACTCTACAGGGAGTAGAGAAGAATCTGGACGAGAACTGCACGAAGAATTCCATTTATCCGGAGCTGTGTTTCTTCCCACAGTATGCATTGAAGTTCATCACAACACCTTTCTTCATATTGAACTCCGCCTATGATGTTTACCAGTTCCATCACATATTGGTGCCGGCCTCAGCAGATCCACACGGACATTGGAGCCGTTGCAAATTGAATCCGGCAGCATGCAACCCTGAACAGTTAAATACATTGCAAGAATACAGGCGCGATATGATCGTTGCGATGGGATTGTTCTACAAATATTCCAGAAGAGGAGGGATTTTCCTCAATTCATGCTTTGCTCATTGCCAAAGTGAGTCTCAGGACACATGGTTTGGTGATGATTCTCCAAGGGTGCATAATAAGACAATTGCAGAAGCAGTAGGTGACTGGTACTTCGGCAGAAGAGTAACAAAGGAAATTGACTGTCCATATCCTTGTGACACTACCTGCCATAACCTCATCCCATCAGCTCAGGCCGCTTTAGAGGATACAAAAAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04310 map04310
Pfam Domains
Protein Families
PAE

Protein Analysis

409

Amino Acids

45.83

Weight (kDa)

8.22

Isoelectric Point (pI)

43.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 26 - 393 6.2e-163 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1181
AccB1I GGYRCC 1 cut(s) 850
AccBSI CCGCTC 1 cut(s) 82
AccII CGCG 3 cut(s) 114, 268, 951
AccIII TCCGGA 1 cut(s) 748
AciI CCGC 4 cut(s) 82, 114, 816, 1203
AclWI GGATC 2 cut(s) 283, 860
AcsI RAATTY 2 cut(s) 649, 736
AcyI GRCGYC 1 cut(s) 618
AfaI GTAC 1 cut(s) 1118
AfiI CCNNNNNNNGG 1 cut(s) 81
AflIII ACRYGT 1 cut(s) 281
AgsI TTSAA 4 cut(s) 655, 781, 811, 898
AjnI CCWGG 1 cut(s) 21
AluBI AGCT 9 cut(s) 41, 122, 131, 373, 392, 570, 754, 1196, 1224
AluI AGCT 9 cut(s) 41, 122, 131, 373, 392, 570, 754, 1196, 1224
Alw26I GTCTC 1 cut(s) 1047
AlwI GGATC 2 cut(s) 283, 860
Aor13HI TCCGGA 1 cut(s) 748
AoxI GGCC 3 cut(s) 183, 855, 1200
ApeKI GCWGC 1 cut(s) 905
ApoI RAATTY 2 cut(s) 649, 736
ArsI GACNNNNNNTTYG 4 cut(s) 1040, 1072, 1103, 1135
AspLEI GCGC 2 cut(s) 56, 951
AspS9I GGNCC 1 cut(s) 183
AsuHPI GGTGA 3 cut(s) 103, 1073, 1121
BanI GGYRCC 1 cut(s) 850
BbvCI CCTCAGC 2 cut(s) 320, 859
BbvI GCAGC 1 cut(s) 917
BccI CCATC 5 cut(s) 137, 380, 846, 961, 1198
BceAI ACGGC 2 cut(s) 14, 870
BciT130I CCWGG 1 cut(s) 23
BciVI GTATCC 1 cut(s) 1207
BcoDI GTCTC 1 cut(s) 1047
BfmI CTRYAG 1 cut(s) 695
BfuAI ACCTGC 1 cut(s) 1181
BfuI GTATCC 1 cut(s) 1207
BglII AGATCT 1 cut(s) 682
BisI GCNGC 3 cut(s) 83, 906, 1203
BlsI GCNGC 3 cut(s) 84, 907, 1204
Bme1390I CCNGG 1 cut(s) 23
BmgT120I GGNCC 1 cut(s) 183
BmiI GGNNCC 4 cut(s) 184, 362, 852, 884
BmrFI CCNGG 1 cut(s) 23
BmsI GCATC 3 cut(s) 341, 397, 557
BoxI GACNNNNGTC 1 cut(s) 104
BpmI CTGGAG 1 cut(s) 423
Bpu10I CCTNAGC 3 cut(s) 320, 859, 1197
BpuEI CTTGAG 1 cut(s) 631
BsaAI YACGTR 1 cut(s) 509
BsaHI GRCGYC 1 cut(s) 618
BsaJI CCNNGG 3 cut(s) 59, 75, 1073
BsaWI WCCGGW 1 cut(s) 748
BsaXI ACNNNNNCTCC 2 cut(s) 396, 426
Bsc4I CCNNNNNNNGG 1 cut(s) 81
Bse118I RCCGGY 1 cut(s) 853
Bse1I ACTGG 5 cut(s) 203, 362, 597, 832, 1118
Bse3DI GCAATG 3 cut(s) 238, 932, 1027
BseAI TCCGGA 1 cut(s) 748
BseBI CCWGG 1 cut(s) 23
BseDI CCNNGG 3 cut(s) 59, 75, 1073
BseGI GGATG 3 cut(s) 148, 471, 1185
BseLI CCNNNNNNNGG 1 cut(s) 81
BseMI GCAATG 3 cut(s) 238, 932, 1027
BseMII CTCAG 4 cut(s) 334, 873, 1058, 1211
BseNI ACTGG 5 cut(s) 203, 362, 597, 832, 1118
BseRI GAGGAG 3 cut(s) 48, 92, 1011
BseXI GCAGC 1 cut(s) 917
BseYI CCCAGC 1 cut(s) 536
BsgI GTGCAG 2 cut(s) 152, 712
Bsh1236I CGCG 3 cut(s) 114, 268, 951
BshFI GGCC 3 cut(s) 185, 857, 1202
BshNI GGYRCC 1 cut(s) 850
BsiSI CCGG 5 cut(s) 186, 621, 749, 854, 902
BslFI GGGAC 1 cut(s) 439
BslI CCNNNNNNNGG 1 cut(s) 81
BsmAI GTCTC 1 cut(s) 1047
BsmFI GGGAC 1 cut(s) 439
BsnI GGCC 3 cut(s) 185, 857, 1202
Bsp13I TCCGGA 1 cut(s) 748
Bsp143I GATC 5 cut(s) 275, 478, 682, 865, 957
BspACI CCGC 4 cut(s) 82, 114, 816, 1203
BspANI GGCC 3 cut(s) 185, 857, 1202
BspCNI CTCAG 4 cut(s) 333, 872, 1057, 1210
BspEI TCCGGA 1 cut(s) 748
BspFNI CGCG 3 cut(s) 114, 268, 951
BspLI GGNNCC 4 cut(s) 184, 362, 852, 884
BspMI ACCTGC 1 cut(s) 1181
BspPI GGATC 2 cut(s) 283, 860
BspT107I GGYRCC 1 cut(s) 850
BsrBI CCGCTC 1 cut(s) 82
BsrDI GCAATG 3 cut(s) 238, 932, 1027
BsrFI RCCGGY 1 cut(s) 853
BsrI ACTGG 5 cut(s) 203, 362, 597, 832, 1118
BssAI RCCGGY 1 cut(s) 853
BssECI CCNNGG 3 cut(s) 59, 75, 1073
BssMI GATC 5 cut(s) 275, 478, 682, 865, 957
BssNI GRCGYC 1 cut(s) 618
BssT1I CCWWGG 1 cut(s) 1073
Bst2UI CCWGG 1 cut(s) 23
Bst4CI ACNGT 5 cut(s) 91, 563, 771, 924, 1151
Bst6I CTCTTC 3 cut(s) 265, 988, 1123
BstACI GRCGYC 1 cut(s) 618
BstBAI YACGTR 1 cut(s) 509
BstC8I GCNNGC 6 cut(s) 52, 156, 262, 266, 855, 910
BstDEI CTNAG 5 cut(s) 320, 374, 859, 1044, 1197
BstF5I GGATG 3 cut(s) 148, 471, 1185
BstFNI CGCG 3 cut(s) 114, 268, 951
BstHHI GCGC 2 cut(s) 56, 951
BstKTI GATC 5 cut(s) 278, 481, 685, 868, 960
BstMAI GTCTC 1 cut(s) 1047
BstMBI GATC 5 cut(s) 275, 478, 682, 865, 957
BstMWI GCNNNNNNNGC 6 cut(s) 47, 128, 398, 407, 512, 1202
BstNI CCWGG 1 cut(s) 23
BstNSI RCATGY 1 cut(s) 912
BstPAI GACNNNNGTC 1 cut(s) 104
BstSCI CCNGG 1 cut(s) 21
BstSFI CTRYAG 1 cut(s) 695
BstUI CGCG 3 cut(s) 114, 268, 951
BstV1I GCAGC 1 cut(s) 917
BstX2I RGATCY 2 cut(s) 682, 865
BstYI RGATCY 2 cut(s) 682, 865
BsuI GTATCC 1 cut(s) 1207
BsuRI GGCC 3 cut(s) 185, 857, 1202
BtgZI GCGATG 1 cut(s) 980
BtsCI GGATG 3 cut(s) 148, 471, 1185
BtsIMutI CAGTG 2 cut(s) 559, 604
BveI ACCTGC 1 cut(s) 1181
Cac8I GCNNGC 6 cut(s) 52, 156, 262, 266, 855, 910
CfoI GCGC 2 cut(s) 56, 951
Cfr10I RCCGGY 1 cut(s) 853
Cfr13I GGNCC 1 cut(s) 183
CseI GACGC 2 cut(s) 120, 626
Csp6I GTAC 1 cut(s) 1117
CviAII CATG 6 cut(s) 97, 246, 664, 909, 1017, 1053
CviQI GTAC 1 cut(s) 1117
DdeI CTNAG 5 cut(s) 320, 374, 859, 1044, 1197
DpnI GATC 5 cut(s) 277, 480, 684, 867, 959
DpnII GATC 5 cut(s) 275, 478, 682, 865, 957
Eam1104I CTCTTC 3 cut(s) 265, 988, 1123
EarI CTCTTC 3 cut(s) 265, 988, 1123
EciI GGCGGA 1 cut(s) 805
Eco130I CCWWGG 1 cut(s) 1073
EcoRI GAATTC 1 cut(s) 736
EcoRII CCWGG 1 cut(s) 21
EcoT14I CCWWGG 1 cut(s) 1073
EcoT22I ATGCAT 2 cut(s) 334, 778
ErhI CCWWGG 1 cut(s) 1073
FaeI CATG 6 cut(s) 100, 249, 667, 912, 1020, 1056
FaqI GGGAC 1 cut(s) 439
FatI CATG 6 cut(s) 96, 245, 663, 908, 1016, 1052
Fnu4HI GCNGC 3 cut(s) 83, 906, 1203
FokI GGATG 3 cut(s) 155, 458, 1172
Fsp4HI GCNGC 3 cut(s) 83, 906, 1203
GlaI GCGC 2 cut(s) 55, 950
GluI GCNGC 3 cut(s) 83, 906, 1203
GsaI CCCAGC 1 cut(s) 540
GsuI CTGGAG 1 cut(s) 423
HaeIII GGCC 3 cut(s) 185, 857, 1202
HapII CCGG 5 cut(s) 186, 621, 749, 854, 902
HgaI GACGC 2 cut(s) 120, 626
HhaI GCGC 2 cut(s) 56, 951
Hin1I GRCGYC 1 cut(s) 618
Hin1II CATG 6 cut(s) 100, 249, 667, 912, 1020, 1056
Hin6I GCGC 2 cut(s) 54, 949
HinP1I GCGC 2 cut(s) 54, 949
HindIII AAGCTT 1 cut(s) 371
HinfI GANTC 8 cut(s) 177, 315, 624, 691, 712, 898, 1040, 1067
HpaII CCGG 5 cut(s) 186, 621, 749, 854, 902
HphI GGTGA 3 cut(s) 103, 1073, 1121
Hpy166II GTNNAC 3 cut(s) 94, 497, 829
Hpy188I TCNGA 4 cut(s) 110, 128, 253, 446
Hpy188III TCNNGA 7 cut(s) 312, 344, 632, 716, 749, 990, 1046
Hpy8I GTNNAC 3 cut(s) 94, 497, 829
Hpy99I CGWCG 1 cut(s) 114
HpyCH4III ACNGT 5 cut(s) 91, 563, 771, 924, 1151
HpyCH4IV ACGT 2 cut(s) 283, 508
HpyF10VI GCNNNNNNNGC 6 cut(s) 47, 128, 398, 407, 512, 1202
HpyF3I CTNAG 5 cut(s) 320, 374, 859, 1044, 1197
HpySE526I ACGT 2 cut(s) 283, 508
Hsp92I GRCGYC 1 cut(s) 618
Hsp92II CATG 6 cut(s) 100, 249, 667, 912, 1020, 1056
HspAI GCGC 2 cut(s) 54, 949
Kpn2I TCCGGA 1 cut(s) 748
KroI GCCGGC 1 cut(s) 853
KroNI GCCGGC 1 cut(s) 855
Kzo9I GATC 5 cut(s) 275, 478, 682, 865, 957
LmnI GCTCC 7 cut(s) 61, 79, 128, 188, 389, 751, 882
Lsp1109I GCAGC 1 cut(s) 917
LweI GCATC 3 cut(s) 341, 397, 557
MaeII ACGT 2 cut(s) 283, 508
MaeIII GTNAC 7 cut(s) 31, 563, 614, 688, 1109, 1132, 1163
MalI GATC 5 cut(s) 277, 480, 684, 867, 959
MbiI CCGCTC 1 cut(s) 82
MboI GATC 5 cut(s) 275, 478, 682, 865, 957
MboII GAAGA 8 cut(s) 282, 473, 721, 745, 754, 793, 1005, 1140
MfeI CAATTG 1 cut(s) 1092
MflI RGATCY 2 cut(s) 682, 865
MluCI AATT 6 cut(s) 649, 736, 893, 1012, 1092, 1143
MlyI GAGTC 2 cut(s) 685, 1049
MmeI TCCRAC 1 cut(s) 133
Mph1103I ATGCAT 2 cut(s) 334, 778
MroI TCCGGA 1 cut(s) 748
MroNI GCCGGC 1 cut(s) 853
MseI TTAA 3 cut(s) 339, 417, 926
MslI CAYNNNNRTG 1 cut(s) 848
MspI CCGG 5 cut(s) 186, 621, 749, 854, 902
MspR9I CCNGG 1 cut(s) 23
MunI CAATTG 1 cut(s) 1092
MvaI CCWGG 1 cut(s) 23
MvnI CGCG 3 cut(s) 114, 268, 951
MwoI GCNNNNNNNGC 6 cut(s) 47, 128, 398, 407, 512, 1202
NaeI GCCGGC 1 cut(s) 855
NdeII GATC 5 cut(s) 275, 478, 682, 865, 957
NgoMIV GCCGGC 1 cut(s) 853
NlaIII CATG 6 cut(s) 100, 249, 667, 912, 1020, 1056
NlaIV GGNNCC 4 cut(s) 184, 362, 852, 884
NmeAIII GCCGAG 1 cut(s) 100
NmuCI GTSAC 4 cut(s) 614, 688, 1109, 1163
NsiI ATGCAT 2 cut(s) 334, 778
NspI RCATGY 1 cut(s) 912
PaeI GCATGC 1 cut(s) 912
PcsI WCGNNNNNNNCGW 1 cut(s) 505
PdiI GCCGGC 1 cut(s) 855
PfeI GAWTC 6 cut(s) 177, 315, 624, 712, 898, 1067
PkrI GCNGC 3 cut(s) 84, 907, 1204
PleI GAGTC 2 cut(s) 685, 1048
PpsI GAGTC 2 cut(s) 685, 1048
Ppu21I YACGTR 1 cut(s) 509
PshAI GACNNNNGTC 1 cut(s) 104
Psp6I CCWGG 1 cut(s) 21
PspFI CCCAGC 1 cut(s) 536
PspGI CCWGG 1 cut(s) 21
PspN4I GGNNCC 4 cut(s) 184, 362, 852, 884
PspPI GGNCC 1 cut(s) 183
PsuI RGATCY 2 cut(s) 682, 865
RsaI GTAC 1 cut(s) 1118
RsaNI GTAC 1 cut(s) 1117
RseI CAYNNNNRTG 1 cut(s) 848
SaqAI TTAA 3 cut(s) 339, 417, 926
SatI GCNGC 3 cut(s) 83, 906, 1203
Sau3AI GATC 5 cut(s) 275, 478, 682, 865, 957
Sau96I GGNCC 1 cut(s) 183
SchI GAGTC 2 cut(s) 685, 1049
ScrFI CCNGG 1 cut(s) 23
SfaNI GCATC 3 cut(s) 341, 397, 557
SfcI CTRYAG 1 cut(s) 695
SmiMI CAYNNNNRTG 1 cut(s) 848
SmlI CTYRAG 1 cut(s) 610
SmoI CTYRAG 1 cut(s) 610
SphI GCATGC 1 cut(s) 912
Sse9I AATT 6 cut(s) 649, 736, 893, 1012, 1092, 1143
SsiI CCGC 4 cut(s) 82, 114, 816, 1203
SspI AATATT 1 cut(s) 986
StyD4I CCNGG 1 cut(s) 21
StyI CCWWGG 1 cut(s) 1073
TaaI ACNGT 5 cut(s) 91, 563, 771, 924, 1151
TaiI ACGT 2 cut(s) 286, 511
TaqI TCGA 1 cut(s) 633
TasI AATT 6 cut(s) 649, 736, 893, 1012, 1092, 1143
TauI GCSGC 2 cut(s) 85, 1205
TfiI GAWTC 6 cut(s) 177, 315, 624, 712, 898, 1067
Tru1I TTAA 3 cut(s) 339, 417, 926
Tru9I TTAA 3 cut(s) 339, 417, 926
TscAI CASTG 2 cut(s) 566, 604
TseFI GTSAC 4 cut(s) 614, 688, 1109, 1163
TseI GCWGC 1 cut(s) 905
Tsp45I GTSAC 4 cut(s) 614, 688, 1109, 1163
TspDTI ATGAA 5 cut(s) 384, 651, 775, 793, 1005
TspGWI ACGGA 1 cut(s) 888
TspRI CASTG 2 cut(s) 566, 604
XapI RAATTY 2 cut(s) 649, 736
XceI RCATGY 1 cut(s) 912
XcmI CCANNNNNNNNNTGG 1 cut(s) 876
Zsp2I ATGCAT 2 cut(s) 334, 778
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.