RLG00000026508

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0007825
Physical Location & Seq
Forward (+)
3455655 .. 3460462
4808 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000026508

Sequence Viewer

Length: 1242 bp
ATGGTAGCAGTCACGTTGGCGCTGCTAACGTGCGCGCCTTGGTGCATTTACTCACAGCAGCCACAGCCACAGCCGCACCCGCTTATGGTGAACATGACCACCGTCCAAAATGCCCCCGCTCTAGGCGCTTTTTGCTTGGATGGGAGTTTGCCTGCGTATCATATTCACAGAGGCTTCGGCGCCGGAGCAAGAAACTGGCTTTTACAGTTTGAGGGTGGAGGGTGGTGTAATGATATAGAGTCTTGCTTGGAGAGGGCGAAAACAAGAAGAGGTTCTACACGTCTTATGAGCAAGTGGGAGGTCTTTTCTGGGATTCTAAGTAATAATGCTTCTCGTAATCCAGATTTTTACAATTGGAACCGTGTGAAGATTCGATATTGTGATGGAGCTTCATTTGCCGGAGACGCAGTGTATAAGAATGGGACAACGTTGCTCTATTTCAGAGGGCAAAAGATTTGGGAAGCCATCATTCTTGATCTTCTGCCTAAAGGTTTAGGAAAGGCACGTAAGGCTTTGTTATCAGGTTGCTCTGCTGGGGGGTTAGCAGCATTTTTACATTGTGACGACTTCACTAAGTATGTACCAAGTAATGCCAGTGTAAAATGCTTGAGTGATGCTGGTTTCTTTCTTGATGAAAGAGACATAGGTTTGAATCACACAATGAGGTCTTTCTATAAAGACCTTGTCTCTCTACAGGGGGTAGAAAAGAATCTGGATAAGAACTGCACCGGATCCCTTCCTTTTCCTGAGTTGTGTATTTTCCCACAGTATGCATTGAGATTCATCACACCGCCATTTTTCATCTTGAACTCGGCTTATGATGTTTACCAGTTCCATCATGGATTGGTGCCATCTTCAGCTGATCCACGCGGACACTGGAACCGTTGCAAACTGGATCCGGCAGCATGCAATCCTGCTCAGTTAAATGTATTGCAAGGTTTCAGGCGCGACATGCTCGTTGCTTTGGCATTGTTCTACAAGTACTCAGCTAGAGGAGGAATGTACATCAACTCATGCTTTGCTCACTGCCAAAGTGAGTCCCAGGACACATGGTTTGGGGTTGATTCTCCAAGAATACACAACAAGACCATTGCAGAAGCTGTAGGTGACTGGTACTTCAGCAGAAGGATAACTAATAAGGAAGTTGACTGTGCATACCCTTGTGACACTACCTGCCATAACCTTATACCAGCAAACACAAGCTCAGGTCTTGTAGAAGATCTGAATTTAGTCGAAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04310 map04310
Pfam Domains
Protein Families
PAE

Protein Analysis

414

Amino Acids

46.06

Weight (kDa)

7.81

Isoelectric Point (pI)

49.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 26 - 393 1.3e-161 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1181
AccB1I GGYRCC 2 cut(s) 179, 847
AccBSI CCGCTC 1 cut(s) 119
AccII CGCG 3 cut(s) 35, 870, 948
AciI CCGC 5 cut(s) 74, 80, 117, 791, 870
AclI AACGTT 1 cut(s) 428
AclWI GGATC 5 cut(s) 726, 739, 857, 890, 903
AcsI RAATTY 1 cut(s) 1225
AcuI CTGAAG 2 cut(s) 840, 1102
AcyI GRCGYC 1 cut(s) 180
AfaI GTAC 4 cut(s) 582, 983, 1004, 1115
AfiI CCNNNNNNNGG 2 cut(s) 85, 122
AflIII ACRYGT 1 cut(s) 278
AgsI TTSAA 2 cut(s) 652, 808
AjiI CACGTC 1 cut(s) 281
AjnI CCWGG 1 cut(s) 1041
AluBI AGCT 5 cut(s) 389, 860, 989, 1100, 1203
AluI AGCT 5 cut(s) 389, 860, 989, 1100, 1203
Alw26I GTCTC 3 cut(s) 396, 633, 691
AlwI GGATC 5 cut(s) 726, 739, 857, 890, 903
AlwNI CAGNNNCTG 1 cut(s) 1100
ApeKI GCWGC 4 cut(s) 22, 58, 545, 902
ApoI RAATTY 1 cut(s) 1225
ArsI GACNNNNNNTTYG 2 cut(s) 1037, 1069
Asp700I GAANNNNTTC 1 cut(s) 271
AspLEI GCGC 6 cut(s) 22, 35, 37, 128, 182, 948
AsuHPI GGTGA 2 cut(s) 100, 1118
BamHI GGATCC 2 cut(s) 731, 895
BanI GGYRCC 2 cut(s) 179, 847
BarI GAAGNNNNNNTAC 4 cut(s) 259, 291, 313, 345
BbvI GCAGC 4 cut(s) 9, 70, 557, 914
BccI CCATC 5 cut(s) 134, 377, 473, 843, 859
BciT130I CCWGG 1 cut(s) 1043
BcoDI GTCTC 3 cut(s) 396, 633, 691
BfaI CTAG 2 cut(s) 122, 990
BfmI CTRYAG 2 cut(s) 692, 1101
BfoI RGCGCY 3 cut(s) 23, 129, 183
BfuAI ACCTGC 1 cut(s) 1181
BglII AGATCT 1 cut(s) 1219
BisI GCNGC 5 cut(s) 23, 59, 74, 546, 903
BlsI GCNGC 5 cut(s) 24, 60, 75, 547, 904
BmcAI AGTACT 1 cut(s) 983
Bme1390I CCNGG 1 cut(s) 1043
BmgBI CACGTC 1 cut(s) 281
BmiI GGNNCC 6 cut(s) 181, 359, 733, 849, 881, 897
BmrFI CCNGG 1 cut(s) 1043
BmsI GCATC 1 cut(s) 604
BoxI GACNNNNGTC 1 cut(s) 101
Bpu10I CCTNAGC 1 cut(s) 1204
BpuEI CTTGAG 1 cut(s) 628
BsaAI YACGTR 1 cut(s) 506
BsaHI GRCGYC 1 cut(s) 180
BsaJI CCNNGG 2 cut(s) 38, 1041
BsaWI WCCGGW 1 cut(s) 728
BsaXI ACNNNNNCTCC 2 cut(s) 393, 423
Bsc4I CCNNNNNNNGG 2 cut(s) 85, 122
Bse1I ACTGG 6 cut(s) 200, 594, 829, 881, 897, 1115
Bse3DI GCAATG 1 cut(s) 1089
BseBI CCWGG 1 cut(s) 1043
BseDI CCNNGG 2 cut(s) 38, 1041
BseGI GGATG 1 cut(s) 145
BseLI CCNNNNNNNGG 2 cut(s) 85, 122
BseMI GCAATG 1 cut(s) 1089
BseMII CTCAG 4 cut(s) 738, 932, 999, 1218
BseNI ACTGG 6 cut(s) 200, 594, 829, 881, 897, 1115
BsePI GCGCGC 1 cut(s) 33
BseRI GAGGAG 1 cut(s) 1008
BseXI GCAGC 4 cut(s) 9, 70, 557, 914
BseYI CCCAGC 1 cut(s) 533
BsgI GTGCAG 1 cut(s) 709
Bsh1236I CGCG 3 cut(s) 35, 870, 948
BshNI GGYRCC 2 cut(s) 179, 847
BsiSI CCGG 4 cut(s) 183, 399, 729, 899
BslFI GGGAC 2 cut(s) 436, 1024
BslI CCNNNNNNNGG 2 cut(s) 85, 122
BsmAI GTCTC 3 cut(s) 396, 633, 691
BsmBI CGTCTC 1 cut(s) 396
BsmFI GGGAC 2 cut(s) 436, 1024
Bsp1407I TGTACA 1 cut(s) 1002
Bsp143I GATC 5 cut(s) 475, 731, 862, 895, 1219
BspACI CCGC 5 cut(s) 74, 80, 117, 791, 870
BspCNI CTCAG 4 cut(s) 739, 931, 998, 1217
BspFNI CGCG 3 cut(s) 35, 870, 948
BspLI GGNNCC 6 cut(s) 181, 359, 733, 849, 881, 897
BspMI ACCTGC 1 cut(s) 1181
BspPI GGATC 5 cut(s) 726, 739, 857, 890, 903
BspT107I GGYRCC 2 cut(s) 179, 847
BsrBI CCGCTC 1 cut(s) 119
BsrDI GCAATG 1 cut(s) 1089
BsrGI TGTACA 1 cut(s) 1002
BsrI ACTGG 6 cut(s) 200, 594, 829, 881, 897, 1115
BssECI CCNNGG 2 cut(s) 38, 1041
BssHII GCGCGC 1 cut(s) 33
BssMI GATC 5 cut(s) 475, 731, 862, 895, 1219
BssNI GRCGYC 1 cut(s) 180
BssT1I CCWWGG 1 cut(s) 38
Bst2UI CCWGG 1 cut(s) 1043
Bst4CI ACNGT 6 cut(s) 103, 207, 362, 768, 884, 1151
Bst6I CTCTTC 1 cut(s) 262
BstACI GRCGYC 1 cut(s) 180
BstAUI TGTACA 1 cut(s) 1002
BstBAI YACGTR 1 cut(s) 506
BstC8I GCNNGC 3 cut(s) 35, 153, 907
BstDEI CTNAG 6 cut(s) 317, 573, 747, 918, 985, 1204
BstF5I GGATG 1 cut(s) 145
BstFNI CGCG 3 cut(s) 35, 870, 948
BstH2I RGCGCY 3 cut(s) 23, 129, 183
BstHHI GCGC 6 cut(s) 22, 35, 37, 128, 182, 948
BstKTI GATC 5 cut(s) 478, 734, 865, 898, 1222
BstMAI GTCTC 3 cut(s) 396, 633, 691
BstMBI GATC 5 cut(s) 475, 731, 862, 895, 1219
BstMWI GCNNNNNNNGC 9 cut(s) 64, 73, 79, 125, 132, 395, 404, 509, 952
BstNI CCWGG 1 cut(s) 1043
BstNSI RCATGY 2 cut(s) 909, 955
BstPAI GACNNNNGTC 1 cut(s) 101
BstSCI CCNGG 1 cut(s) 1041
BstSFI CTRYAG 2 cut(s) 692, 1101
BstUI CGCG 3 cut(s) 35, 870, 948
BstV1I GCAGC 4 cut(s) 9, 70, 557, 914
BstX2I RGATCY 3 cut(s) 731, 895, 1219
BstYI RGATCY 3 cut(s) 731, 895, 1219
BtrI CACGTC 1 cut(s) 281
BtsCI GGATG 1 cut(s) 145
BtsI GCAGTG 2 cut(s) 414, 1024
BtsIMutI CAGTG 4 cut(s) 414, 601, 874, 1024
BveI ACCTGC 1 cut(s) 1181
Cac8I GCNNGC 3 cut(s) 35, 153, 907
CaiI CAGNNNCTG 1 cut(s) 1100
CfoI GCGC 6 cut(s) 22, 35, 37, 128, 182, 948
CseI GACGC 1 cut(s) 413
Csp6I GTAC 4 cut(s) 581, 982, 1003, 1114
CviAII CATG 6 cut(s) 94, 839, 906, 952, 1014, 1050
CviQI GTAC 4 cut(s) 581, 982, 1003, 1114
DdeI CTNAG 6 cut(s) 317, 573, 747, 918, 985, 1204
DinI GGCGCC 1 cut(s) 181
DpnI GATC 5 cut(s) 477, 733, 864, 897, 1221
DpnII GATC 5 cut(s) 475, 731, 862, 895, 1219
Eam1104I CTCTTC 1 cut(s) 262
EarI CTCTTC 1 cut(s) 262
Eco130I CCWWGG 1 cut(s) 38
Eco57I CTGAAG 2 cut(s) 840, 1102
EcoRII CCWGG 1 cut(s) 1041
EcoT14I CCWWGG 1 cut(s) 38
EcoT22I ATGCAT 1 cut(s) 775
EgeI GGCGCC 1 cut(s) 181
EheI GGCGCC 1 cut(s) 181
ErhI CCWWGG 1 cut(s) 38
Esp3I CGTCTC 1 cut(s) 396
FaeI CATG 6 cut(s) 97, 842, 909, 955, 1017, 1053
FaqI GGGAC 2 cut(s) 436, 1024
FatI CATG 6 cut(s) 93, 838, 905, 951, 1013, 1049
FauI CCCGC 2 cut(s) 87, 124
Fnu4HI GCNGC 5 cut(s) 23, 59, 74, 546, 903
FokI GGATG 1 cut(s) 152
Fsp4HI GCNGC 5 cut(s) 23, 59, 74, 546, 903
FspBI CTAG 2 cut(s) 122, 990
GlaI GCGC 6 cut(s) 21, 34, 36, 127, 181, 947
GluI GCNGC 5 cut(s) 23, 59, 74, 546, 903
GsaI CCCAGC 1 cut(s) 537
HaeII RGCGCY 3 cut(s) 23, 129, 183
HapII CCGG 4 cut(s) 183, 399, 729, 899
HgaI GACGC 1 cut(s) 413
HhaI GCGC 6 cut(s) 22, 35, 37, 128, 182, 948
Hin1I GRCGYC 1 cut(s) 180
Hin1II CATG 6 cut(s) 97, 842, 909, 955, 1017, 1053
Hin6I GCGC 6 cut(s) 20, 33, 35, 126, 180, 946
HinP1I GCGC 6 cut(s) 20, 33, 35, 126, 180, 946
HincII GTYRAC 1 cut(s) 1147
HindII GTYRAC 1 cut(s) 1147
HinfI GANTC 8 cut(s) 239, 313, 370, 652, 709, 780, 1037, 1064
HpaII CCGG 4 cut(s) 183, 399, 729, 899
HphI GGTGA 2 cut(s) 100, 1118
Hpy166II GTNNAC 3 cut(s) 91, 826, 1147
Hpy188I TCNGA 2 cut(s) 443, 1224
Hpy188III TCNNGA 6 cut(s) 341, 473, 629, 713, 746, 805
Hpy8I GTNNAC 3 cut(s) 91, 826, 1147
HpyAV CCTTC 2 cut(s) 746, 1119
HpyCH4III ACNGT 6 cut(s) 103, 207, 362, 768, 884, 1151
HpyCH4IV ACGT 5 cut(s) 14, 29, 280, 428, 505
HpyCH4V TGCA 8 cut(s) 45, 726, 773, 888, 909, 934, 1094, 1154
HpyF10VI GCNNNNNNNGC 9 cut(s) 64, 73, 79, 125, 132, 395, 404, 509, 952
HpyF3I CTNAG 6 cut(s) 317, 573, 747, 918, 985, 1204
HpySE526I ACGT 5 cut(s) 14, 29, 280, 428, 505
Hsp92I GRCGYC 1 cut(s) 180
Hsp92II CATG 6 cut(s) 97, 842, 909, 955, 1017, 1053
HspAI GCGC 6 cut(s) 20, 33, 35, 126, 180, 946
KasI GGCGCC 1 cut(s) 179
Kzo9I GATC 5 cut(s) 475, 731, 862, 895, 1219
LmnI GCTCC 2 cut(s) 185, 386
Lsp1109I GCAGC 4 cut(s) 9, 70, 557, 914
LweI GCATC 1 cut(s) 604
MaeI CTAG 2 cut(s) 122, 990
MaeII ACGT 5 cut(s) 14, 29, 280, 428, 505
MaeIII GTNAC 4 cut(s) 10, 560, 1106, 1163
MalI GATC 5 cut(s) 477, 733, 864, 897, 1221
MbiI CCGCTC 1 cut(s) 119
MboI GATC 5 cut(s) 475, 731, 862, 895, 1219
MboII GAAGA 5 cut(s) 279, 379, 470, 846, 1229
MfeI CAATTG 1 cut(s) 352
MflI RGATCY 3 cut(s) 731, 895, 1219
MluCI AATT 2 cut(s) 352, 1225
Mly113I GGCGCC 1 cut(s) 180
MlyI GAGTC 2 cut(s) 248, 1046
Mph1103I ATGCAT 1 cut(s) 775
MroXI GAANNNNTTC 1 cut(s) 271
MseI TTAA 1 cut(s) 923
MspA1I CMGCKG 1 cut(s) 860
MspI CCGG 4 cut(s) 183, 399, 729, 899
MspR9I CCNGG 1 cut(s) 1043
MunI CAATTG 1 cut(s) 352
MvaI CCWGG 1 cut(s) 1043
MvnI CGCG 3 cut(s) 35, 870, 948
MwoI GCNNNNNNNGC 9 cut(s) 64, 73, 79, 125, 132, 395, 404, 509, 952
NarI GGCGCC 1 cut(s) 180
NdeII GATC 5 cut(s) 475, 731, 862, 895, 1219
NlaIII CATG 6 cut(s) 97, 842, 909, 955, 1017, 1053
NlaIV GGNNCC 6 cut(s) 181, 359, 733, 849, 881, 897
NmeAIII GCCGAG 1 cut(s) 791
NmuCI GTSAC 4 cut(s) 10, 560, 1106, 1163
NsiI ATGCAT 1 cut(s) 775
NspI RCATGY 2 cut(s) 909, 955
PaeI GCATGC 1 cut(s) 909
PauI GCGCGC 1 cut(s) 33
PdmI GAANNNNTTC 1 cut(s) 271
PfeI GAWTC 6 cut(s) 313, 370, 652, 709, 780, 1064
PflFI GACNNNGTC 1 cut(s) 683
PkrI GCNGC 5 cut(s) 24, 60, 75, 547, 904
PleI GAGTC 2 cut(s) 247, 1045
PluTI GGCGCC 1 cut(s) 183
PpsI GAGTC 2 cut(s) 247, 1045
Ppu21I YACGTR 1 cut(s) 506
PshAI GACNNNNGTC 1 cut(s) 101
Psp1406I AACGTT 1 cut(s) 428
Psp6I CCWGG 1 cut(s) 1041
PspFI CCCAGC 1 cut(s) 533
PspGI CCWGG 1 cut(s) 1041
PspN4I GGNNCC 6 cut(s) 181, 359, 733, 849, 881, 897
PstNI CAGNNNCTG 1 cut(s) 1100
PsuI RGATCY 3 cut(s) 731, 895, 1219
PsyI GACNNNGTC 1 cut(s) 683
PteI GCGCGC 1 cut(s) 33
PvuII CAGCTG 1 cut(s) 860
RsaI GTAC 4 cut(s) 582, 983, 1004, 1115
RsaNI GTAC 4 cut(s) 581, 982, 1003, 1114
SaqAI TTAA 1 cut(s) 923
SatI GCNGC 5 cut(s) 23, 59, 74, 546, 903
Sau3AI GATC 5 cut(s) 475, 731, 862, 895, 1219
ScaI AGTACT 1 cut(s) 983
SchI GAGTC 2 cut(s) 248, 1046
ScrFI CCNGG 1 cut(s) 1043
SfaNI GCATC 1 cut(s) 604
SfcI CTRYAG 2 cut(s) 692, 1101
SfoI GGCGCC 1 cut(s) 181
SmlI CTYRAG 1 cut(s) 607
SmoI CTYRAG 1 cut(s) 607
SphI GCATGC 1 cut(s) 909
Sse9I AATT 2 cut(s) 352, 1225
SsiI CCGC 5 cut(s) 74, 80, 117, 791, 870
SspDI GGCGCC 1 cut(s) 179
SspMI CTAG 2 cut(s) 122, 990
StyD4I CCNGG 1 cut(s) 1041
StyI CCWWGG 1 cut(s) 38
TaaI ACNGT 6 cut(s) 103, 207, 362, 768, 884, 1151
TaiI ACGT 5 cut(s) 17, 32, 283, 431, 508
TaqI TCGA 2 cut(s) 373, 1233
TasI AATT 2 cut(s) 352, 1225
TatI WGTACW 2 cut(s) 981, 1002
TauI GCSGC 1 cut(s) 76
TfiI GAWTC 6 cut(s) 313, 370, 652, 709, 780, 1064
Tru1I TTAA 1 cut(s) 923
Tru9I TTAA 1 cut(s) 923
TscAI CASTG 4 cut(s) 414, 601, 881, 1031
TseFI GTSAC 4 cut(s) 10, 560, 1106, 1163
TseI GCWGC 4 cut(s) 22, 58, 545, 902
Tsp45I GTSAC 4 cut(s) 10, 560, 1106, 1163
TspDTI ATGAA 4 cut(s) 381, 648, 772, 790
TspRI CASTG 4 cut(s) 414, 601, 881, 1031
Tth111I GACNNNGTC 1 cut(s) 683
XapI RAATTY 1 cut(s) 1225
XceI RCATGY 2 cut(s) 909, 955
XcmI CCANNNNNNNNNTGG 2 cut(s) 836, 873
XmnI GAANNNNTTC 1 cut(s) 271
XspI CTAG 2 cut(s) 122, 990
ZrmI AGTACT 1 cut(s) 983
Zsp2I ATGCAT 1 cut(s) 775
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.