Prupe.1G518100_v2.0.a1

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0007825
Physical Location & Seq
Forward (+)
42568118 .. 42569876
1759 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G518100.1

Sequence Viewer

Length: 1197 bp
ATGAATGTGAACGCAGTACTAGTCGCCATCACCCTGGCTCTGCTAACTTGCGGGCCTCGGTGCATTTACTCGGACGACCAGCGGCTCCTGGTGAACATGACTCTGGTCCGGAATGCTTCGGCTCTCGGAGCTTTTTGCTTGGATGGGAGTTTGCCTGCGTATCACCTGCACAGAGGATTCGGGGGTGGAGCAAGAAACTGGCTTTTACAGTTTGAAGGTGGAGGGTGGTGCAATAGCACAAAATCATGCTTGGACAGAGCCAACACCCGTAGAGGATCAACACGCTACATGAGCAAGTGGGAGGTCTTCTCTGGAATTCTAAGCAATAATGCTTCTCTAAATCCAGATTTTTACAATTGGAACCGTGTGAAGCTTAGATATTGTGATGGAGCTTCATTTGCTGGAGATGCAGTATATAAAAACGGGACGTCGTTGCTCTATTTCAGAGGGCAAAAGATTTGGGAAGCAATCATTCTTGATCTTCTGCCAAAAGGTTTAGGACAGGCAAGAAAGGCTCTGCTATCAGGTTCTTCTGCTGGGGGTTTATCATCCTTTTTGCATTGTAACAACTTCACTAAATATTTACCAAGCACTGCCAGTGTGAAATGCTTGAGCGATGCCGGATTCTTTCTTGACGAAAGAGATATAAGTTCTAACCACACCATGAGGTCTTTCGTGAAAGATGTTGTTTCTCTACACGGGGTAGAGAAGAATCTGGATGAGAACTGCACCGCCAGCTACCTTAATTTTCCCGAGCTGTGTTTCTTCCCACAGTACGCAATGAAGTTCATTACACCACCTTTTTTCATATTGAACTCAGCTTATGATGTTTACCAGTTCCATCATATATTGGTGCCGCCTTCAGCTGATCCACAAGGCCACTGGAATCGTTGCAAACTGAATCCGGCAGCGTGCAATCCTCAACAGTTAAATGCATTGCAAGAATTCAGGCGTGGCATGATTGTTGCTGTGGGATTGTCCTACAAATATTCGAGACGAGGAGGCTTCTTCATTAATTCATGCTTTGCTCACGGCCAAAGTGAGTCTCAAGACACGTGGTTTGCTGCAGACTCTCCAAGGGTTCACAATAGGACAATTGCAGAAGCAGTAGGTGATTGGTACTTCAACAGAAGGGCAACAAAGGAAATTGACTGCCCATATCCTTGTGTCACTACCTGCCATAACCTTATACCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04310 map04310
Pfam Domains
Protein Families
PAE

Protein Analysis

399

Amino Acids

44.47

Weight (kDa)

8.8

Isoelectric Point (pI)

43.36

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 174
AatII GACGTC 1 cut(s) 431
Acc36I ACCTGC 2 cut(s) 174, 1184
AccB1I GGYRCC 1 cut(s) 853
AccIII TCCGGA 1 cut(s) 108
AciI CCGC 4 cut(s) 51, 82, 732, 857
AclWI GGATC 2 cut(s) 283, 863
AcoI YGGCCR 1 cut(s) 1033
AcsI RAATTY 2 cut(s) 315, 944
AcuI CTGAAG 1 cut(s) 846
AcvI CACGTG 1 cut(s) 1056
AcyI GRCGYC 1 cut(s) 428
AfaI GTAC 3 cut(s) 18, 776, 1121
AflIII ACRYGT 1 cut(s) 1053
AgsI TTSAA 3 cut(s) 215, 814, 1126
AhlI ACTAGT 1 cut(s) 19
AjnI CCWGG 2 cut(s) 33, 87
AluBI AGCT 7 cut(s) 131, 373, 392, 738, 757, 821, 866
AluI AGCT 7 cut(s) 131, 373, 392, 738, 757, 821, 866
Alw26I GTCTC 2 cut(s) 988, 1050
AlwI GGATC 2 cut(s) 283, 863
Ama87I CYCGRG 1 cut(s) 752
Aor13HI TCCGGA 1 cut(s) 108
AoxI GGCC 3 cut(s) 53, 877, 1033
ApeKI GCWGC 2 cut(s) 908, 1064
ApoI RAATTY 2 cut(s) 315, 944
ArsI GACNNNNNNTTYG 2 cut(s) 1043, 1075
AseI ATTAAT 1 cut(s) 1014
AspS9I GGNCC 2 cut(s) 53, 106
AsuHPI GGTGA 4 cut(s) 22, 103, 155, 1124
AvaI CYCGRG 1 cut(s) 752
AvaII GGWCC 1 cut(s) 106
BanI GGYRCC 1 cut(s) 853
BbrPI CACGTG 1 cut(s) 1056
BbsI GAAGAC 1 cut(s) 298
BbvI GCAGC 2 cut(s) 920, 1051
BccI CCATC 4 cut(s) 35, 137, 380, 849
BceAI ACGGC 1 cut(s) 1048
BciT130I CCWGG 2 cut(s) 35, 89
BcoDI GTCTC 2 cut(s) 988, 1050
BcuI ACTAGT 1 cut(s) 19
BfaI CTAG 1 cut(s) 20
BfmI CTRYAG 1 cut(s) 1065
BfuAI ACCTGC 2 cut(s) 174, 1184
BisI GCNGC 4 cut(s) 83, 857, 909, 1065
BlsI GCNGC 4 cut(s) 84, 858, 910, 1066
BmcAI AGTACT 1 cut(s) 18
Bme1390I CCNGG 2 cut(s) 35, 89
Bme18I GGWCC 1 cut(s) 106
BmeT110I CYCGRG 1 cut(s) 752
BmgT120I GGNCC 2 cut(s) 53, 106
BmiI GGNNCC 3 cut(s) 86, 362, 855
BmrFI CCNGG 2 cut(s) 35, 89
BmsI GCATC 2 cut(s) 397, 607
BoxI GACNNNNGTC 1 cut(s) 104
BpiI GAAGAC 1 cut(s) 298
BplI GAGNNNNNCTC 2 cut(s) 293, 325
BpmI CTGGAG 1 cut(s) 423
BpuEI CTTGAG 2 cut(s) 631, 1032
BsaAI YACGTR 1 cut(s) 1056
BsaHI GRCGYC 1 cut(s) 428
BsaJI CCNNGG 3 cut(s) 33, 56, 1076
BsaWI WCCGGW 1 cut(s) 108
BsaXI ACNNNNNCTCC 2 cut(s) 396, 426
Bse1I ACTGG 4 cut(s) 203, 597, 835, 887
Bse3DI GCAATG 2 cut(s) 786, 935
BseAI TCCGGA 1 cut(s) 108
BseBI CCWGG 2 cut(s) 35, 89
BseDI CCNNGG 3 cut(s) 33, 56, 1076
BseGI GGATG 3 cut(s) 148, 548, 724
BseMI GCAATG 2 cut(s) 786, 935
BseMII CTCAG 1 cut(s) 831
BseNI ACTGG 4 cut(s) 203, 597, 835, 887
BseRI GAGGAG 1 cut(s) 1014
BseXI GCAGC 2 cut(s) 920, 1051
BseYI CCCAGC 1 cut(s) 536
BsgI GTGCAG 2 cut(s) 152, 712
BshFI GGCC 3 cut(s) 55, 879, 1035
BshNI GGYRCC 1 cut(s) 853
BsiHKCI CYCGRG 1 cut(s) 752
BsiSI CCGG 3 cut(s) 109, 621, 905
BslFI GGGAC 1 cut(s) 439
BsmAI GTCTC 2 cut(s) 988, 1050
BsmBI CGTCTC 1 cut(s) 988
BsmFI GGGAC 1 cut(s) 439
BsmI GAATGC 1 cut(s) 118
BsnI GGCC 3 cut(s) 55, 879, 1035
BsoBI CYCGRG 1 cut(s) 752
Bsp13I TCCGGA 1 cut(s) 108
Bsp143I GATC 3 cut(s) 275, 478, 868
BspACI CCGC 4 cut(s) 51, 82, 732, 857
BspANI GGCC 3 cut(s) 55, 879, 1035
BspCNI CTCAG 1 cut(s) 830
BspEI TCCGGA 1 cut(s) 108
BspLI GGNNCC 3 cut(s) 86, 362, 855
BspMAI CTGCAG 1 cut(s) 1069
BspMI ACCTGC 2 cut(s) 174, 1184
BspPI GGATC 2 cut(s) 283, 863
BspT107I GGYRCC 1 cut(s) 853
BsrDI GCAATG 2 cut(s) 786, 935
BsrI ACTGG 4 cut(s) 203, 597, 835, 887
BssECI CCNNGG 3 cut(s) 33, 56, 1076
BssMI GATC 3 cut(s) 275, 478, 868
BssNI GRCGYC 1 cut(s) 428
BssT1I CCWWGG 1 cut(s) 1076
Bst2UI CCWGG 2 cut(s) 35, 89
Bst4CI ACNGT 4 cut(s) 210, 365, 774, 927
BstACI GRCGYC 1 cut(s) 428
BstBAI YACGTR 1 cut(s) 1056
BstC8I GCNNGC 4 cut(s) 53, 156, 736, 913
BstDEI CTNAG 3 cut(s) 320, 374, 817
BstF5I GGATG 3 cut(s) 148, 548, 724
BstKTI GATC 3 cut(s) 278, 481, 871
BstMAI GTCTC 2 cut(s) 988, 1050
BstMBI GATC 3 cut(s) 275, 478, 868
BstMWI GCNNNNNNNGC 6 cut(s) 128, 291, 398, 407, 512, 735
BstNI CCWGG 2 cut(s) 35, 89
BstPAI GACNNNNGTC 1 cut(s) 104
BstSCI CCNGG 2 cut(s) 33, 87
BstSFI CTRYAG 1 cut(s) 1065
BstV1I GCAGC 2 cut(s) 920, 1051
BstV2I GAAGAC 1 cut(s) 298
BstXI CCANNNNNNTGG 1 cut(s) 34
BsuRI GGCC 3 cut(s) 55, 879, 1035
BtgZI GCGATG 1 cut(s) 630
BtsCI GGATG 3 cut(s) 148, 548, 724
BtsI GCAGTG 1 cut(s) 591
BtsIMutI CAGTG 3 cut(s) 591, 604, 880
BveI ACCTGC 2 cut(s) 174, 1184
Cac8I GCNNGC 4 cut(s) 53, 156, 736, 913
Cfr13I GGNCC 2 cut(s) 53, 106
Csp6I GTAC 3 cut(s) 17, 775, 1120
CspCI CAANNNNNGTGG 2 cut(s) 1037, 1072
CviAII CATG 6 cut(s) 97, 246, 289, 664, 958, 1020
CviQI GTAC 3 cut(s) 17, 775, 1120
DdeI CTNAG 3 cut(s) 320, 374, 817
DpnI GATC 3 cut(s) 277, 480, 870
DpnII GATC 3 cut(s) 275, 478, 868
EaeI YGGCCR 1 cut(s) 1033
Eco130I CCWWGG 1 cut(s) 1076
Eco47I GGWCC 1 cut(s) 106
Eco57I CTGAAG 1 cut(s) 846
Eco72I CACGTG 1 cut(s) 1056
Eco88I CYCGRG 1 cut(s) 752
EcoRI GAATTC 2 cut(s) 315, 944
EcoRII CCWGG 2 cut(s) 33, 87
EcoT14I CCWWGG 1 cut(s) 1076
EcoT22I ATGCAT 1 cut(s) 937
ErhI CCWWGG 1 cut(s) 1076
Esp3I CGTCTC 1 cut(s) 988
FaeI CATG 6 cut(s) 100, 249, 292, 667, 961, 1023
FaqI GGGAC 1 cut(s) 439
FatI CATG 6 cut(s) 96, 245, 288, 663, 957, 1019
FauI CCCGC 1 cut(s) 44
Fnu4HI GCNGC 4 cut(s) 83, 857, 909, 1065
FokI GGATG 3 cut(s) 155, 535, 731
Fsp4HI GCNGC 4 cut(s) 83, 857, 909, 1065
FspBI CTAG 1 cut(s) 20
GluI GCNGC 4 cut(s) 83, 857, 909, 1065
GsaI CCCAGC 1 cut(s) 540
GsuI CTGGAG 1 cut(s) 423
HaeIII GGCC 3 cut(s) 55, 879, 1035
HapII CCGG 3 cut(s) 109, 621, 905
Hin1I GRCGYC 1 cut(s) 428
Hin1II CATG 6 cut(s) 100, 249, 292, 667, 961, 1023
HindIII AAGCTT 1 cut(s) 371
HinfI GANTC 8 cut(s) 100, 177, 624, 712, 886, 901, 1043, 1070
HpaII CCGG 3 cut(s) 109, 621, 905
HphI GGTGA 4 cut(s) 22, 103, 155, 1124
Hpy166II GTNNAC 4 cut(s) 10, 94, 832, 1084
Hpy188I TCNGA 3 cut(s) 73, 128, 446
Hpy8I GTNNAC 4 cut(s) 10, 94, 832, 1084
Hpy99I CGWCG 1 cut(s) 433
HpyAV CCTTC 3 cut(s) 209, 870, 1125
HpyCH4III ACNGT 4 cut(s) 210, 365, 774, 927
HpyCH4IV ACGT 2 cut(s) 428, 1055
HpyF10VI GCNNNNNNNGC 6 cut(s) 128, 291, 398, 407, 512, 735
HpyF3I CTNAG 3 cut(s) 320, 374, 817
HpySE526I ACGT 2 cut(s) 428, 1055
Hsp92I GRCGYC 1 cut(s) 428
Hsp92II CATG 6 cut(s) 100, 249, 292, 667, 961, 1023
Kpn2I TCCGGA 1 cut(s) 108
Kzo9I GATC 3 cut(s) 275, 478, 868
LmnI GCTCC 4 cut(s) 90, 128, 188, 389
Lsp1109I GCAGC 2 cut(s) 920, 1051
LweI GCATC 2 cut(s) 397, 607
MaeI CTAG 1 cut(s) 20
MaeII ACGT 2 cut(s) 428, 1055
MaeIII GTNAC 2 cut(s) 563, 1168
MalI GATC 3 cut(s) 277, 480, 870
MboI GATC 3 cut(s) 275, 478, 868
MboII GAAGA 6 cut(s) 298, 473, 522, 721, 757, 1000
MfeI CAATTG 2 cut(s) 355, 1095
MluCI AATT 7 cut(s) 315, 355, 745, 944, 1015, 1095, 1146
MlyI GAGTC 3 cut(s) 94, 1052, 1064
Mph1103I ATGCAT 1 cut(s) 937
MroI TCCGGA 1 cut(s) 108
MseI TTAA 3 cut(s) 744, 929, 1014
MspA1I CMGCKG 2 cut(s) 82, 866
MspI CCGG 3 cut(s) 109, 621, 905
MspR9I CCNGG 2 cut(s) 35, 89
MunI CAATTG 2 cut(s) 355, 1095
Mva1269I GAATGC 1 cut(s) 118
MvaI CCWGG 2 cut(s) 35, 89
MwoI GCNNNNNNNGC 6 cut(s) 128, 291, 398, 407, 512, 735
NdeII GATC 3 cut(s) 275, 478, 868
NlaIII CATG 6 cut(s) 100, 249, 292, 667, 961, 1023
NlaIV GGNNCC 3 cut(s) 86, 362, 855
NmuCI GTSAC 1 cut(s) 1168
NsiI ATGCAT 1 cut(s) 937
PaqCI CACCTGC 1 cut(s) 174
PctI GAATGC 1 cut(s) 118
PfeI GAWTC 5 cut(s) 177, 624, 712, 886, 901
PkrI GCNGC 4 cut(s) 84, 858, 910, 1066
PleI GAGTC 3 cut(s) 94, 1051, 1064
PmaCI CACGTG 1 cut(s) 1056
PmlI CACGTG 1 cut(s) 1056
PpsI GAGTC 3 cut(s) 94, 1051, 1064
Ppu21I YACGTR 1 cut(s) 1056
PshAI GACNNNNGTC 1 cut(s) 104
PshBI ATTAAT 1 cut(s) 1014
Psp6I CCWGG 2 cut(s) 33, 87
PspCI CACGTG 1 cut(s) 1056
PspFI CCCAGC 1 cut(s) 536
PspGI CCWGG 2 cut(s) 33, 87
PspN4I GGNNCC 3 cut(s) 86, 362, 855
PspPI GGNCC 2 cut(s) 53, 106
PstI CTGCAG 1 cut(s) 1069
PvuII CAGCTG 1 cut(s) 866
RsaI GTAC 3 cut(s) 18, 776, 1121
RsaNI GTAC 3 cut(s) 17, 775, 1120
SaqAI TTAA 3 cut(s) 744, 929, 1014
SatI GCNGC 4 cut(s) 83, 857, 909, 1065
Sau3AI GATC 3 cut(s) 275, 478, 868
Sau96I GGNCC 2 cut(s) 53, 106
ScaI AGTACT 1 cut(s) 18
SchI GAGTC 3 cut(s) 94, 1052, 1064
ScrFI CCNGG 2 cut(s) 35, 89
SfaNI GCATC 2 cut(s) 397, 607
SfcI CTRYAG 1 cut(s) 1065
SinI GGWCC 1 cut(s) 106
SmlI CTYRAG 2 cut(s) 610, 1047
SmoI CTYRAG 2 cut(s) 610, 1047
SpeI ACTAGT 1 cut(s) 19
Sse9I AATT 7 cut(s) 315, 355, 745, 944, 1015, 1095, 1146
SsiI CCGC 4 cut(s) 51, 82, 732, 857
SspI AATATT 2 cut(s) 581, 989
SspMI CTAG 1 cut(s) 20
StyD4I CCNGG 2 cut(s) 33, 87
StyI CCWWGG 1 cut(s) 1076
TaaI ACNGT 4 cut(s) 210, 365, 774, 927
TaiI ACGT 2 cut(s) 431, 1058
TaqI TCGA 1 cut(s) 992
TasI AATT 7 cut(s) 315, 355, 745, 944, 1015, 1095, 1146
TatI WGTACW 1 cut(s) 16
TauI GCSGC 2 cut(s) 85, 859
TfiI GAWTC 5 cut(s) 177, 624, 712, 886, 901
Tru1I TTAA 3 cut(s) 744, 929, 1014
Tru9I TTAA 3 cut(s) 744, 929, 1014
TscAI CASTG 3 cut(s) 598, 604, 887
TseFI GTSAC 1 cut(s) 1168
TseI GCWGC 2 cut(s) 908, 1064
Tsp45I GTSAC 1 cut(s) 1168
TspDTI ATGAA 7 cut(s) 17, 384, 778, 796, 797, 1000, 1008
TspRI CASTG 3 cut(s) 598, 604, 887
VpaK11BI GGWCC 1 cut(s) 106
VspI ATTAAT 1 cut(s) 1014
XapI RAATTY 2 cut(s) 315, 944
XcmI CCANNNNNNNNNTGG 1 cut(s) 879
XspI CTAG 1 cut(s) 20
ZraI GACGTC 1 cut(s) 429
ZrmI AGTACT 1 cut(s) 18
Zsp2I ATGCAT 1 cut(s) 937
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.