Rw1G037010

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0007825
Physical Location & Seq
Reverse (-)
64774368 .. 64779771
5404 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G037010.1

Sequence Viewer

Length: 1272 bp
ATGGTTACTGAAATGAAGACGACGATGACGAAGAGCGTGAATGCGGTGGTAGCAGTCACGTTGGCGCTGCTAACGTGCGCGCCTTGGTGCATTTACTCACAGCAGCCGCACCAGCTTATGGTGAACATGACCACCGTCCGAAATGCCCCCGCTCTCGGCGCTTTTTGCTTGGATGGGAGTTTGCCTGCGTATCATATTCACAGAGGCTTCGGCGCCGGAGCAAGAAACTGGCTTTTACAGTTTGAGGGTGGAGGGTGGTGTAATGATATAGAGTCTTGCTTGGAGAGGGCGAAAACAAGAAGAGGTTCTACACACCTTATGAGCAAGTGGGAGGTCTTTTCTGGGATTCTAAGTAATAATGCTTCTCTTAATCCAGATTTTTACAATTGGAACCGTGTGAAGATTCGATATTGTGATGGAGCTTCATTTGCCGGAGACGCAGTGTATAAGAATGGGACAACGTTGCTCTATTTCAGAGGGCAAAAGATTTGGGAAGCCATCATTCTTGATCTTCTGCCTAAAGGTTTAGGAAAGGCACGTAAGGCTTTGTTATCAGGTTGCTCTGCTGGGGGTTTAGCAGCATTTTTACATTGTGATGACTTCACTAAGTATGTACCAAGTAATGCCAGTGTAAAATGCTTGAGTGATGCTGGATTCTTTCTTGATGAAAGAGACATAGGTTTGAATCACACAATGAGGTCTTTCTATAAAGACCTTGTCTCTCTACAGGGTGTAGCAAAGAATCTGGATAAGAACTGCACCGGATCCCTTCCTTTTCCTGAGTTGTGTATTTTCCCACAGTATGCATTGAGATTCATCACACCGCCTTTTTTCATCTTGAACTCGGCTTATGATGTTTACCAGTTCCATCATGGATTGGTGCCATCTTCAGCTGATCCACGCGGACACTGGAACCGTTGCAAACTGGATCCGGCAGCATGCAATCCTGCTCAGTTAGTATTGCAAGGTTTCAGGCGCGACATGCTTGTTGCTTTGGCATTGTTCTACAAGTACTCGGCAAGAGGGGGGATGTACATCAACTCATGCTTTGCTCACTGCCAAAGTGAGTCCCAGGACACATGGTTTGGGGTTGATTCTCCAAGAATACACAACAAGACCATTGCAGAAGCTGTAGGTGACTGGTATTTTAGCAGAAGGATAACTAATAAGGAAGTTGACTGTGCATACCCTTGTGACACTACCTGCCATAACCTTATACCAGCAAACACAAGCTCAGGTCTTGTAGAAGATCTCAATTTAGTCGAAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04310 map04310
Pfam Domains
Protein Families
PAE

Protein Analysis

423

Amino Acids

47.05

Weight (kDa)

8.05

Isoelectric Point (pI)

46.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 36 - 403 6.6e-159 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1211
AccB1I GGYRCC 2 cut(s) 212, 880
AccB7I CCANNNNNTGG 1 cut(s) 118
AccBSI CCGCTC 1 cut(s) 152
AccII CGCG 3 cut(s) 80, 903, 978
AciI CCGC 5 cut(s) 44, 107, 150, 824, 903
AclI AACGTT 1 cut(s) 461
AclWI GGATC 5 cut(s) 759, 772, 890, 923, 936
AcuI CTGAAG 1 cut(s) 873
AcyI GRCGYC 1 cut(s) 213
AfaI GTAC 3 cut(s) 615, 1013, 1034
AfiI CCNNNNNNNGG 2 cut(s) 118, 155
AgsI TTSAA 2 cut(s) 685, 841
AjnI CCWGG 1 cut(s) 1071
AluBI AGCT 5 cut(s) 115, 422, 893, 1130, 1233
AluI AGCT 5 cut(s) 115, 422, 893, 1130, 1233
Alw26I GTCTC 3 cut(s) 429, 666, 724
AlwI GGATC 5 cut(s) 759, 772, 890, 923, 936
AlwNI CAGNNNCTG 1 cut(s) 1130
ApeKI GCWGC 4 cut(s) 67, 103, 578, 935
ArsI GACNNNNNNTTYG 2 cut(s) 1067, 1099
Asp700I GAANNNNTTC 1 cut(s) 304
AspLEI GCGC 6 cut(s) 67, 80, 82, 161, 215, 978
AsuHPI GGTGA 2 cut(s) 133, 1148
BamHI GGATCC 2 cut(s) 764, 928
BanI GGYRCC 2 cut(s) 212, 880
BarI GAAGNNNNNNTAC 4 cut(s) 292, 324, 346, 378
BbsI GAAGAC 1 cut(s) 23
BbvI GCAGC 4 cut(s) 54, 115, 590, 947
BccI CCATC 5 cut(s) 167, 410, 506, 876, 892
BciT130I CCWGG 1 cut(s) 1073
BcoDI GTCTC 3 cut(s) 429, 666, 724
BfmI CTRYAG 2 cut(s) 725, 1131
BfoI RGCGCY 3 cut(s) 68, 162, 216
BfuAI ACCTGC 1 cut(s) 1211
BglII AGATCT 1 cut(s) 1249
BisI GCNGC 5 cut(s) 68, 104, 107, 579, 936
BlsI GCNGC 5 cut(s) 69, 105, 108, 580, 937
BmcAI AGTACT 1 cut(s) 1013
Bme1390I CCNGG 1 cut(s) 1073
BmiI GGNNCC 6 cut(s) 214, 392, 766, 882, 914, 930
BmrFI CCNGG 1 cut(s) 1073
BmsI GCATC 1 cut(s) 637
BoxI GACNNNNGTC 1 cut(s) 134
BpiI GAAGAC 1 cut(s) 23
Bpu10I CCTNAGC 1 cut(s) 1234
BpuEI CTTGAG 1 cut(s) 661
BsaAI YACGTR 1 cut(s) 539
BsaBI GATNNNNATC 1 cut(s) 1034
BsaHI GRCGYC 1 cut(s) 213
BsaJI CCNNGG 2 cut(s) 83, 1071
BsaWI WCCGGW 1 cut(s) 761
BsaXI ACNNNNNCTCC 2 cut(s) 426, 456
Bsc4I CCNNNNNNNGG 2 cut(s) 118, 155
Bse1I ACTGG 6 cut(s) 233, 627, 862, 914, 930, 1145
Bse3DI GCAATG 1 cut(s) 1119
Bse8I GATNNNNATC 1 cut(s) 1034
BseBI CCWGG 1 cut(s) 1073
BseDI CCNNGG 2 cut(s) 83, 1071
BseGI GGATG 2 cut(s) 178, 1035
BseJI GATNNNNATC 1 cut(s) 1034
BseLI CCNNNNNNNGG 2 cut(s) 118, 155
BseMI GCAATG 1 cut(s) 1119
BseMII CTCAG 3 cut(s) 771, 965, 1248
BseNI ACTGG 6 cut(s) 233, 627, 862, 914, 930, 1145
BsePI GCGCGC 1 cut(s) 78
BseXI GCAGC 4 cut(s) 54, 115, 590, 947
BseYI CCCAGC 1 cut(s) 566
BsgI GTGCAG 1 cut(s) 742
Bsh1236I CGCG 3 cut(s) 80, 903, 978
BshNI GGYRCC 2 cut(s) 212, 880
BsiSI CCGG 4 cut(s) 216, 432, 762, 932
BslFI GGGAC 2 cut(s) 469, 1054
BslI CCNNNNNNNGG 2 cut(s) 118, 155
BsmAI GTCTC 3 cut(s) 429, 666, 724
BsmBI CGTCTC 1 cut(s) 429
BsmFI GGGAC 2 cut(s) 469, 1054
BsmI GAATGC 1 cut(s) 46
Bsp1407I TGTACA 1 cut(s) 1032
Bsp143I GATC 5 cut(s) 508, 764, 895, 928, 1249
BspACI CCGC 5 cut(s) 44, 107, 150, 824, 903
BspCNI CTCAG 3 cut(s) 772, 964, 1247
BspFNI CGCG 3 cut(s) 80, 903, 978
BspLI GGNNCC 6 cut(s) 214, 392, 766, 882, 914, 930
BspMI ACCTGC 1 cut(s) 1211
BspPI GGATC 5 cut(s) 759, 772, 890, 923, 936
BspQI GCTCTTC 1 cut(s) 26
BspT107I GGYRCC 2 cut(s) 212, 880
BsrBI CCGCTC 1 cut(s) 152
BsrDI GCAATG 1 cut(s) 1119
BsrGI TGTACA 1 cut(s) 1032
BsrI ACTGG 6 cut(s) 233, 627, 862, 914, 930, 1145
BssECI CCNNGG 2 cut(s) 83, 1071
BssHII GCGCGC 1 cut(s) 78
BssMI GATC 5 cut(s) 508, 764, 895, 928, 1249
BssNI GRCGYC 1 cut(s) 213
BssT1I CCWWGG 1 cut(s) 83
Bst2UI CCWGG 1 cut(s) 1073
Bst4CI ACNGT 6 cut(s) 136, 240, 395, 801, 917, 1181
Bst6I CTCTTC 2 cut(s) 26, 295
BstACI GRCGYC 1 cut(s) 213
BstAUI TGTACA 1 cut(s) 1032
BstBAI YACGTR 1 cut(s) 539
BstC8I GCNNGC 3 cut(s) 80, 186, 940
BstDEI CTNAG 5 cut(s) 350, 606, 780, 951, 1234
BstF5I GGATG 2 cut(s) 178, 1035
BstFNI CGCG 3 cut(s) 80, 903, 978
BstH2I RGCGCY 3 cut(s) 68, 162, 216
BstHHI GCGC 6 cut(s) 67, 80, 82, 161, 215, 978
BstKTI GATC 5 cut(s) 511, 767, 898, 931, 1252
BstMAI GTCTC 3 cut(s) 429, 666, 724
BstMBI GATC 5 cut(s) 508, 764, 895, 928, 1249
BstMWI GCNNNNNNNGC 8 cut(s) 50, 112, 158, 165, 428, 437, 542, 982
BstNI CCWGG 1 cut(s) 1073
BstNSI RCATGY 2 cut(s) 942, 985
BstPAI GACNNNNGTC 1 cut(s) 134
BstSCI CCNGG 1 cut(s) 1071
BstSFI CTRYAG 2 cut(s) 725, 1131
BstUI CGCG 3 cut(s) 80, 903, 978
BstV1I GCAGC 4 cut(s) 54, 115, 590, 947
BstV2I GAAGAC 1 cut(s) 23
BstX2I RGATCY 3 cut(s) 764, 928, 1249
BstYI RGATCY 3 cut(s) 764, 928, 1249
BtsCI GGATG 2 cut(s) 178, 1035
BtsI GCAGTG 2 cut(s) 447, 1054
BtsIMutI CAGTG 4 cut(s) 447, 634, 907, 1054
BveI ACCTGC 1 cut(s) 1211
Cac8I GCNNGC 3 cut(s) 80, 186, 940
CaiI CAGNNNCTG 1 cut(s) 1130
CfoI GCGC 6 cut(s) 67, 80, 82, 161, 215, 978
CseI GACGC 1 cut(s) 446
Csp6I GTAC 3 cut(s) 614, 1012, 1033
CviAII CATG 6 cut(s) 127, 872, 939, 982, 1044, 1080
CviQI GTAC 3 cut(s) 614, 1012, 1033
DdeI CTNAG 5 cut(s) 350, 606, 780, 951, 1234
DinI GGCGCC 1 cut(s) 214
DpnI GATC 5 cut(s) 510, 766, 897, 930, 1251
DpnII GATC 5 cut(s) 508, 764, 895, 928, 1249
Eam1104I CTCTTC 2 cut(s) 26, 295
EarI CTCTTC 2 cut(s) 26, 295
Eco130I CCWWGG 1 cut(s) 83
Eco57I CTGAAG 1 cut(s) 873
EcoRII CCWGG 1 cut(s) 1071
EcoT14I CCWWGG 1 cut(s) 83
EcoT22I ATGCAT 1 cut(s) 808
EgeI GGCGCC 1 cut(s) 214
EheI GGCGCC 1 cut(s) 214
ErhI CCWWGG 1 cut(s) 83
Esp3I CGTCTC 1 cut(s) 429
FaeI CATG 6 cut(s) 130, 875, 942, 985, 1047, 1083
FaqI GGGAC 2 cut(s) 469, 1054
FatI CATG 6 cut(s) 126, 871, 938, 981, 1043, 1079
FauI CCCGC 1 cut(s) 157
Fnu4HI GCNGC 5 cut(s) 68, 104, 107, 579, 936
FokI GGATG 2 cut(s) 185, 1042
Fsp4HI GCNGC 5 cut(s) 68, 104, 107, 579, 936
GlaI GCGC 6 cut(s) 66, 79, 81, 160, 214, 977
GluI GCNGC 5 cut(s) 68, 104, 107, 579, 936
GsaI CCCAGC 1 cut(s) 570
HaeII RGCGCY 3 cut(s) 68, 162, 216
HapII CCGG 4 cut(s) 216, 432, 762, 932
HgaI GACGC 1 cut(s) 446
HhaI GCGC 6 cut(s) 67, 80, 82, 161, 215, 978
Hin1I GRCGYC 1 cut(s) 213
Hin1II CATG 6 cut(s) 130, 875, 942, 985, 1047, 1083
Hin6I GCGC 6 cut(s) 65, 78, 80, 159, 213, 976
HinP1I GCGC 6 cut(s) 65, 78, 80, 159, 213, 976
HincII GTYRAC 1 cut(s) 1177
HindII GTYRAC 1 cut(s) 1177
HinfI GANTC 9 cut(s) 272, 346, 403, 654, 685, 742, 813, 1067, 1094
HpaII CCGG 4 cut(s) 216, 432, 762, 932
HphI GGTGA 2 cut(s) 133, 1148
Hpy166II GTNNAC 3 cut(s) 124, 859, 1177
Hpy188I TCNGA 2 cut(s) 140, 476
Hpy188III TCNNGA 6 cut(s) 374, 506, 662, 746, 779, 838
Hpy8I GTNNAC 3 cut(s) 124, 859, 1177
Hpy99I CGWCG 1 cut(s) 25
HpyAV CCTTC 2 cut(s) 779, 1149
HpyCH4III ACNGT 6 cut(s) 136, 240, 395, 801, 917, 1181
HpyCH4IV ACGT 4 cut(s) 59, 74, 461, 538
HpyCH4V TGCA 8 cut(s) 90, 759, 806, 921, 942, 964, 1124, 1184
HpyF10VI GCNNNNNNNGC 8 cut(s) 50, 112, 158, 165, 428, 437, 542, 982
HpyF3I CTNAG 5 cut(s) 350, 606, 780, 951, 1234
HpySE526I ACGT 4 cut(s) 59, 74, 461, 538
Hsp92I GRCGYC 1 cut(s) 213
Hsp92II CATG 6 cut(s) 130, 875, 942, 985, 1047, 1083
HspAI GCGC 6 cut(s) 65, 78, 80, 159, 213, 976
KasI GGCGCC 1 cut(s) 212
Kzo9I GATC 5 cut(s) 508, 764, 895, 928, 1249
LguI GCTCTTC 1 cut(s) 26
LmnI GCTCC 2 cut(s) 218, 419
Lsp1109I GCAGC 4 cut(s) 54, 115, 590, 947
LweI GCATC 1 cut(s) 637
MaeII ACGT 4 cut(s) 59, 74, 461, 538
MaeIII GTNAC 4 cut(s) 4, 55, 1136, 1193
MalI GATC 5 cut(s) 510, 766, 897, 930, 1251
MbiI CCGCTC 1 cut(s) 152
MboI GATC 5 cut(s) 508, 764, 895, 928, 1249
MboII GAAGA 7 cut(s) 28, 43, 312, 412, 503, 879, 1259
MfeI CAATTG 1 cut(s) 385
MflI RGATCY 3 cut(s) 764, 928, 1249
MluCI AATT 2 cut(s) 385, 1255
Mly113I GGCGCC 1 cut(s) 213
MlyI GAGTC 2 cut(s) 281, 1076
MnlI CCTC 9 cut(s) 197, 238, 245, 279, 296, 325, 470, 690, 1016
Mph1103I ATGCAT 1 cut(s) 808
MroXI GAANNNNTTC 1 cut(s) 304
MseI TTAA 1 cut(s) 369
MslI CAYNNNNRTG 1 cut(s) 594
MspA1I CMGCKG 1 cut(s) 893
MspI CCGG 4 cut(s) 216, 432, 762, 932
MspR9I CCNGG 1 cut(s) 1073
MunI CAATTG 1 cut(s) 385
Mva1269I GAATGC 1 cut(s) 46
MvaI CCWGG 1 cut(s) 1073
MvnI CGCG 3 cut(s) 80, 903, 978
MwoI GCNNNNNNNGC 8 cut(s) 50, 112, 158, 165, 428, 437, 542, 982
NarI GGCGCC 1 cut(s) 213
NdeII GATC 5 cut(s) 508, 764, 895, 928, 1249
NlaIII CATG 6 cut(s) 130, 875, 942, 985, 1047, 1083
NlaIV GGNNCC 6 cut(s) 214, 392, 766, 882, 914, 930
NmeAIII GCCGAG 3 cut(s) 135, 824, 995
NmuCI GTSAC 3 cut(s) 55, 1136, 1193
NsiI ATGCAT 1 cut(s) 808
NspI RCATGY 2 cut(s) 942, 985
PaeI GCATGC 1 cut(s) 942
PauI GCGCGC 1 cut(s) 78
PciSI GCTCTTC 1 cut(s) 26
PcsI WCGNNNNNNNCGW 1 cut(s) 26
PctI GAATGC 1 cut(s) 46
PdmI GAANNNNTTC 1 cut(s) 304
PfeI GAWTC 7 cut(s) 346, 403, 654, 685, 742, 813, 1094
PflFI GACNNNGTC 1 cut(s) 716
PflMI CCANNNNNTGG 1 cut(s) 118
PkrI GCNGC 5 cut(s) 69, 105, 108, 580, 937
PleI GAGTC 2 cut(s) 280, 1075
PluTI GGCGCC 1 cut(s) 216
PpsI GAGTC 2 cut(s) 280, 1075
Ppu21I YACGTR 1 cut(s) 539
PshAI GACNNNNGTC 1 cut(s) 134
Psp1406I AACGTT 1 cut(s) 461
Psp6I CCWGG 1 cut(s) 1071
PspFI CCCAGC 1 cut(s) 566
PspGI CCWGG 1 cut(s) 1071
PspN4I GGNNCC 6 cut(s) 214, 392, 766, 882, 914, 930
PstNI CAGNNNCTG 1 cut(s) 1130
PsuI RGATCY 3 cut(s) 764, 928, 1249
PsyI GACNNNGTC 1 cut(s) 716
PteI GCGCGC 1 cut(s) 78
PvuII CAGCTG 1 cut(s) 893
RsaI GTAC 3 cut(s) 615, 1013, 1034
RsaNI GTAC 3 cut(s) 614, 1012, 1033
RseI CAYNNNNRTG 1 cut(s) 594
SapI GCTCTTC 1 cut(s) 26
SaqAI TTAA 1 cut(s) 369
SatI GCNGC 5 cut(s) 68, 104, 107, 579, 936
Sau3AI GATC 5 cut(s) 508, 764, 895, 928, 1249
ScaI AGTACT 1 cut(s) 1013
SchI GAGTC 2 cut(s) 281, 1076
ScrFI CCNGG 1 cut(s) 1073
SfaNI GCATC 1 cut(s) 637
SfcI CTRYAG 2 cut(s) 725, 1131
SfoI GGCGCC 1 cut(s) 214
SmiMI CAYNNNNRTG 1 cut(s) 594
SmlI CTYRAG 1 cut(s) 640
SmoI CTYRAG 1 cut(s) 640
SphI GCATGC 1 cut(s) 942
Sse9I AATT 2 cut(s) 385, 1255
SsiI CCGC 5 cut(s) 44, 107, 150, 824, 903
SspDI GGCGCC 1 cut(s) 212
StyD4I CCNGG 1 cut(s) 1071
StyI CCWWGG 1 cut(s) 83
TaaI ACNGT 6 cut(s) 136, 240, 395, 801, 917, 1181
TaiI ACGT 4 cut(s) 62, 77, 464, 541
TaqI TCGA 2 cut(s) 406, 1263
TasI AATT 2 cut(s) 385, 1255
TatI WGTACW 2 cut(s) 1011, 1032
TauI GCSGC 1 cut(s) 109
TfiI GAWTC 7 cut(s) 346, 403, 654, 685, 742, 813, 1094
Tru1I TTAA 1 cut(s) 369
Tru9I TTAA 1 cut(s) 369
TscAI CASTG 4 cut(s) 447, 634, 914, 1061
TseFI GTSAC 3 cut(s) 55, 1136, 1193
TseI GCWGC 4 cut(s) 67, 103, 578, 935
Tsp45I GTSAC 3 cut(s) 55, 1136, 1193
TspDTI ATGAA 5 cut(s) 29, 414, 681, 805, 823
TspRI CASTG 4 cut(s) 447, 634, 914, 1061
Tth111I GACNNNGTC 1 cut(s) 716
Van91I CCANNNNNTGG 1 cut(s) 118
XceI RCATGY 2 cut(s) 942, 985
XcmI CCANNNNNNNNNTGG 2 cut(s) 869, 906
XmnI GAANNNNTTC 1 cut(s) 304
ZrmI AGTACT 1 cut(s) 1013
Zsp2I ATGCAT 1 cut(s) 808
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.