AT5G24600

Protein of unknown function, DUF599

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0007447
Physical Location & Seq
Reverse (-)
8421558 .. 8423525
1968 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G24600.2

Sequence Viewer

Length: 747 bp
ATGAAGCGTGAGTATTTAGATTATACACTTGTGCCTTTAGGGCTTGCTTTGATGGTCTTTTATCATCTATGGCTTCTCTACCGTATCATCCACCGTCCTTCTTCGACTGTTGTTGGCCTTAACGCCTTCAATCGCCGCCTTTGGGTCCAAGCCATGATGGAGGACTCATCCAAAAACGGCGTTTTAGCGGTTCAAACGCTAAGGAACAACATAATGGCATCAACTCTATTAGCATCAACCGCAATAATGTTATGTTCACTAATCGCGGTACTAATGACTAGCGCTACAGGAGAGCGATCAGTCTGGTTTGTGTTTGGAGACAAAAGCGACCGAGCCTTCTCGCTCAAGTTCTTCGCAATCCTCGTGTGTTTCCTAGTCGCGTTTCTCCTCAATGTCCAATCAATTCGTTATTACAGCCATGCAAGCATCCTTATCAATGTCCCCTTTAAACAGCTCATGGCTGTTTCTTCAGGTGGTCGTGGTAATGGTAGTCTTATGATAAACCAAGATTATGTGGCTGCAACGGTTAACCGTGGGAGTTACTTCTGGTCGTTAGGACTACGAGCGTTCTACTTCTCCTCGCCTTTATTCCTATGGATCTTTGGTCCCATCCCAATGTTCATAACTTGTTGCGTTCTTGTTTGTTCGTTGTACTTCCTTGATTTAACGTTTGACTCGATGAAATGTAGCGTTGGAGCCGCAGACGCGGAGGAGCCCGAGATTAGAAGCTTAGCTCAGAATGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF599

Protein Analysis

248

Amino Acids

27.84

Weight (kDa)

8.93

Isoelectric Point (pI)

41.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF599 PF04654 10 - 223 3.6e-72 Protein of unknown function, DUF599
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 266, 380, 707
AciI CCGC 6 cut(s) 136, 188, 240, 266, 699, 707
AclI AACGTT 1 cut(s) 668
AclWI GGATC 1 cut(s) 605
AcuI CTGAAG 1 cut(s) 453
AfaI GTAC 2 cut(s) 270, 653
AfeI AGCGCT 1 cut(s) 283
AfiI CCNNNNNNNGG 1 cut(s) 142
AgsI TTSAA 2 cut(s) 130, 194
AluBI AGCT 3 cut(s) 454, 729, 734
AluI AGCT 3 cut(s) 454, 729, 734
Alw26I GTCTC 1 cut(s) 312
AlwI GGATC 1 cut(s) 605
Ama87I CYCGRG 1 cut(s) 716
Aor51HI AGCGCT 1 cut(s) 283
AoxI GGCC 1 cut(s) 115
ApeKI GCWGC 1 cut(s) 518
AspLEI GCGC 1 cut(s) 284
AspS9I GGNCC 2 cut(s) 145, 605
AvaI CYCGRG 1 cut(s) 716
AvaII GGWCC 2 cut(s) 145, 605
BanII GRGCYC 1 cut(s) 717
BauI CACGAG 1 cut(s) 362
BbvI GCAGC 1 cut(s) 505
BccI CCATC 3 cut(s) 46, 151, 617
BceAI ACGGC 1 cut(s) 193
BcoDI GTCTC 1 cut(s) 312
BfaI CTAG 2 cut(s) 279, 374
BfmI CTRYAG 1 cut(s) 285
BfoI RGCGCY 1 cut(s) 285
BglI GCCNNNNNGGC 1 cut(s) 40
BisI GCNGC 3 cut(s) 136, 519, 699
BlpI GCTNAGC 1 cut(s) 730
BlsI GCNGC 3 cut(s) 137, 520, 700
Bme18I GGWCC 2 cut(s) 145, 605
BmeT110I CYCGRG 1 cut(s) 716
BmgT120I GGNCC 2 cut(s) 145, 605
BmiI GGNNCC 4 cut(s) 146, 607, 697, 714
BmsI GCATC 3 cut(s) 227, 242, 435
Bpu10I CCTNAGC 1 cut(s) 200
Bpu1102I GCTNAGC 1 cut(s) 730
BpuEI CTTGAG 1 cut(s) 329
BsaJI CCNNGG 1 cut(s) 532
Bsc4I CCNNNNNNNGG 1 cut(s) 142
BseDI CCNNGG 1 cut(s) 532
BseGI GGATG 4 cut(s) 87, 167, 426, 609
BseLI CCNNNNNNNGG 1 cut(s) 142
BseRI GAGGAG 3 cut(s) 377, 568, 725
BseXI GCAGC 1 cut(s) 505
Bsh1236I CGCG 3 cut(s) 266, 380, 707
Bsh1285I CGRYCG 1 cut(s) 331
BshFI GGCC 1 cut(s) 117
BsiEI CGRYCG 1 cut(s) 331
BsiHKCI CYCGRG 1 cut(s) 716
BslFI GGGAC 2 cut(s) 425, 591
BslI CCNNNNNNNGG 1 cut(s) 142
BsmAI GTCTC 1 cut(s) 312
BsmFI GGGAC 2 cut(s) 425, 591
BsnI GGCC 1 cut(s) 117
BsoBI CYCGRG 1 cut(s) 716
Bsp1286I GDGCHC 1 cut(s) 717
Bsp143I GATC 2 cut(s) 296, 597
Bsp1720I GCTNAGC 1 cut(s) 730
BspACI CCGC 6 cut(s) 136, 188, 240, 266, 699, 707
BspANI GGCC 1 cut(s) 117
BspFNI CGCG 3 cut(s) 266, 380, 707
BspLI GGNNCC 4 cut(s) 146, 607, 697, 714
BspPI GGATC 1 cut(s) 605
BssECI CCNNGG 1 cut(s) 532
BssMI GATC 2 cut(s) 296, 597
BssSI CACGAG 1 cut(s) 362
Bst2BI CACGAG 1 cut(s) 362
Bst4CI ACNGT 5 cut(s) 83, 95, 109, 526, 533
BstC8I GCNNGC 2 cut(s) 45, 424
BstDEI CTNAG 3 cut(s) 200, 730, 735
BstDSI CCRYGG 1 cut(s) 532
BstF5I GGATG 4 cut(s) 87, 167, 426, 609
BstFNI CGCG 3 cut(s) 266, 380, 707
BstH2I RGCGCY 1 cut(s) 285
BstHHI GCGC 1 cut(s) 284
BstKTI GATC 2 cut(s) 299, 600
BstMAI GTCTC 1 cut(s) 312
BstMBI GATC 2 cut(s) 296, 597
BstMCI CGRYCG 1 cut(s) 331
BstMWI GCNNNNNNNGC 4 cut(s) 40, 239, 423, 704
BstSFI CTRYAG 1 cut(s) 285
BstUI CGCG 3 cut(s) 266, 380, 707
BstV1I GCAGC 1 cut(s) 505
BstX2I RGATCY 1 cut(s) 597
BstYI RGATCY 1 cut(s) 597
BsuRI GGCC 1 cut(s) 117
BtgI CCRYGG 1 cut(s) 532
BtsCI GGATG 4 cut(s) 87, 167, 426, 609
Cac8I GCNNGC 2 cut(s) 45, 424
CfoI GCGC 1 cut(s) 284
Cfr13I GGNCC 2 cut(s) 145, 605
CseI GACGC 1 cut(s) 713
Csp6I GTAC 2 cut(s) 269, 652
CviAII CATG 3 cut(s) 154, 419, 457
CviQI GTAC 2 cut(s) 269, 652
DdeI CTNAG 3 cut(s) 200, 730, 735
DpnI GATC 2 cut(s) 298, 599
DpnII GATC 2 cut(s) 296, 597
DraI TTTAAA 1 cut(s) 448
Eco24I GRGCYC 1 cut(s) 717
Eco47I GGWCC 2 cut(s) 145, 605
Eco47III AGCGCT 1 cut(s) 283
Eco57I CTGAAG 1 cut(s) 453
Eco88I CYCGRG 1 cut(s) 716
EcoT38I GRGCYC 1 cut(s) 717
FaeI CATG 3 cut(s) 157, 422, 460
FaqI GGGAC 2 cut(s) 425, 591
FatI CATG 3 cut(s) 153, 418, 456
Fnu4HI GCNGC 3 cut(s) 136, 519, 699
FokI GGATG 4 cut(s) 74, 154, 413, 596
FriOI GRGCYC 1 cut(s) 717
Fsp4HI GCNGC 3 cut(s) 136, 519, 699
FspBI CTAG 2 cut(s) 279, 374
GlaI GCGC 1 cut(s) 283
GluI GCNGC 3 cut(s) 136, 519, 699
HaeII RGCGCY 1 cut(s) 285
HaeIII GGCC 1 cut(s) 117
HgaI GACGC 1 cut(s) 713
HhaI GCGC 1 cut(s) 284
Hin1II CATG 3 cut(s) 157, 422, 460
Hin6I GCGC 1 cut(s) 282
HinP1I GCGC 1 cut(s) 282
HincII GTYRAC 1 cut(s) 529
HindII GTYRAC 1 cut(s) 529
HindIII AAGCTT 1 cut(s) 727
HinfI GANTC 2 cut(s) 164, 674
HpaI GTTAAC 1 cut(s) 529
Hpy166II GTNNAC 2 cut(s) 257, 529
Hpy188I TCNGA 1 cut(s) 738
Hpy8I GTNNAC 2 cut(s) 257, 529
HpyAV CCTTC 3 cut(s) 108, 136, 346
HpyCH4III ACNGT 5 cut(s) 83, 95, 109, 526, 533
HpyCH4IV ACGT 1 cut(s) 668
HpyCH4V TGCA 2 cut(s) 422, 521
HpyF10VI GCNNNNNNNGC 4 cut(s) 40, 239, 423, 704
HpyF3I CTNAG 3 cut(s) 200, 730, 735
HpySE526I ACGT 1 cut(s) 668
Hsp92II CATG 3 cut(s) 157, 422, 460
HspAI GCGC 1 cut(s) 282
KspAI GTTAAC 1 cut(s) 529
Kzo9I GATC 2 cut(s) 296, 597
LmnI GCTCC 2 cut(s) 695, 712
LpnPI CCDG 4 cut(s) 273, 289, 456, 532
Lsp1109I GCAGC 1 cut(s) 505
LweI GCATC 3 cut(s) 227, 242, 435
MaeI CTAG 2 cut(s) 279, 374
MaeII ACGT 1 cut(s) 668
MaeIII GTNAC 1 cut(s) 539
MalI GATC 2 cut(s) 298, 599
MboI GATC 2 cut(s) 296, 597
MboII GAAGA 3 cut(s) 93, 343, 459
MflI RGATCY 1 cut(s) 597
MhlI GDGCHC 1 cut(s) 717
MluCI AATT 1 cut(s) 402
MlyI GAGTC 2 cut(s) 158, 668
MmeI TCCRAC 1 cut(s) 673
MnlI CCTC 5 cut(s) 154, 371, 398, 589, 703
MseI TTAA 4 cut(s) 120, 447, 528, 665
MslI CAYNNNNRTG 1 cut(s) 614
MvnI CGCG 3 cut(s) 266, 380, 707
MwoI GCNNNNNNNGC 4 cut(s) 40, 239, 423, 704
NdeII GATC 2 cut(s) 296, 597
NlaIII CATG 3 cut(s) 157, 422, 460
NlaIV GGNNCC 4 cut(s) 146, 607, 697, 714
PcsI WCGNNNNNNNCGW 2 cut(s) 360, 674
PkrI GCNGC 3 cut(s) 137, 520, 700
PleI GAGTC 2 cut(s) 158, 668
PpsI GAGTC 2 cut(s) 158, 668
Psp1406I AACGTT 1 cut(s) 668
PspN4I GGNNCC 4 cut(s) 146, 607, 697, 714
PspPI GGNCC 2 cut(s) 145, 605
PsuI RGATCY 1 cut(s) 597
RsaI GTAC 2 cut(s) 270, 653
RsaNI GTAC 2 cut(s) 269, 652
RseI CAYNNNNRTG 1 cut(s) 614
SaqAI TTAA 4 cut(s) 120, 447, 528, 665
SatI GCNGC 3 cut(s) 136, 519, 699
Sau3AI GATC 2 cut(s) 296, 597
Sau96I GGNCC 2 cut(s) 145, 605
SchI GAGTC 2 cut(s) 158, 668
SduI GDGCHC 1 cut(s) 717
SetI ASST 5 cut(s) 456, 475, 671, 731, 736
SfaNI GCATC 3 cut(s) 227, 242, 435
SfcI CTRYAG 1 cut(s) 285
SinI GGWCC 2 cut(s) 145, 605
SmiMI CAYNNNNRTG 1 cut(s) 614
SmlI CTYRAG 1 cut(s) 344
SmoI CTYRAG 1 cut(s) 344
Sse9I AATT 1 cut(s) 402
SsiI CCGC 6 cut(s) 136, 188, 240, 266, 699, 707
SspMI CTAG 2 cut(s) 279, 374
TaaI ACNGT 5 cut(s) 83, 95, 109, 526, 533
TaiI ACGT 1 cut(s) 671
TaqI TCGA 2 cut(s) 104, 677
TaqII GACCGA 1 cut(s) 345
TasI AATT 1 cut(s) 402
TatI WGTACW 1 cut(s) 651
TauI GCSGC 2 cut(s) 138, 701
Tru1I TTAA 4 cut(s) 120, 447, 528, 665
Tru9I TTAA 4 cut(s) 120, 447, 528, 665
TseI GCWGC 1 cut(s) 518
TspDTI ATGAA 3 cut(s) 17, 610, 695
VpaK11BI GGWCC 2 cut(s) 145, 605
XspI CTAG 2 cut(s) 279, 374
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.