MD07G1302800.v1.1

Protein of unknown function, DUF599

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0007447
Physical Location & Seq
Reverse (-)
36087859 .. 36090174
2316 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1302800.v1.1.491

Sequence Viewer

Length: 894 bp
ATGTTTGCAATAAAAACCCGCCTCGTTTTTCTCCTTTTCGTACCAAAAAACCAGAGAGTCCACTCCACACACACAAACACAGCTCAACACAACAGAACCCACACCCGTGTTACACTTGGACTCTCTCTCTCCTCCCTTGAACAATTTCAGAAGCAAAAGATCTCAGAAGAAGAAGAAGAAATGCAGCAAAGAGTTTTGGATTACGTTTTGGTGCCGGCAGGGCTGCTGGTAATGGTGGCATACCACCTATGGCTTCTCTATCGAATCCTGACGCAGCCCAACAGCACCGTCATCGGAATCAATTCCATCAACCGCCGCTTCTGGGTTCACGCAATGATGGAGGAAGCTCCAAAGCATGGTGTTCTTGCGGTGCAAACATTGAGAAACAACATAATGGCGTCGACCCTTTTGGCCTCGACCGCCATTACGCTCTGCTCTCTCACTGCCCTCTTGATGAACAGCGGCACCAAATACAGAGCAGCTTTATTTGTCTTTGGGGACCGAAGCGAGATTGCATTTGCGATCAAGTTCTTCGCCATTTTGGTGTGTTTCCTGGTGGCTTTCCTCTTCAATGTGCAGTCCATAAGGTACTACAGCCACGCCAGCATTCTCATCAACGCACCATGCAAGAGGATGTCGCCGCACCATCAGCACCACTACCTCACTACAGACTACGTTGCCACCACCGTGAACCGAGGCAGCTATTTCTGGTCCTTGGGACTCCGGGCGTTCTACTTCTCGTTCCCTTTGTTTCTATGGATTTTCGGGCCAATTCCGATGTTCGTGTCCTGCTTTGTTTTGGTCATCACGCTCTACTTCCTAGATGTCAGCTTTCAGTTTGGTTGGGAGGTTGGGGTTGCTGACAACGATGAGGAACAAAGGTTGCCAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF599

Protein Analysis

298

Amino Acids

34.14

Weight (kDa)

9.19

Isoelectric Point (pI)

41.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF599 PF04654 69 - 278 8.9e-75 Protein of unknown function, DUF599
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 211, 464
AccB7I CCANNNNNTGG 1 cut(s) 356
AccI GTMKAC 1 cut(s) 401
AciI CCGC 7 cut(s) 19, 313, 316, 368, 420, 462, 641
AcyI GRCGYC 1 cut(s) 398
AfaI GTAC 2 cut(s) 42, 590
AfiI CCNNNNNNNGG 2 cut(s) 322, 356
AgsI TTSAA 2 cut(s) 140, 571
AjnI CCWGG 1 cut(s) 552
AleI CACNNNNGTG 2 cut(s) 105, 686
AluBI AGCT 5 cut(s) 83, 347, 482, 702, 831
AluI AGCT 5 cut(s) 83, 347, 482, 702, 831
AoxI GGCC 2 cut(s) 411, 767
ApeKI GCWGC 5 cut(s) 184, 223, 274, 479, 699
Asp700I GAANNNNTTC 2 cut(s) 144, 301
AspS9I GGNCC 3 cut(s) 499, 711, 767
AsuC2I CCSGG 1 cut(s) 725
AvaII GGWCC 2 cut(s) 499, 711
BanI GGYRCC 2 cut(s) 211, 464
BbvI GCAGC 5 cut(s) 196, 210, 286, 491, 711
BccI CCATC 3 cut(s) 314, 331, 654
BcgI CGANNNNNNTGC 2 cut(s) 274, 308
BciT130I CCWGG 1 cut(s) 554
BcnI CCSGG 1 cut(s) 725
BfaI CTAG 2 cut(s) 821, 892
BfmI CTRYAG 2 cut(s) 592, 666
BglI GCCNNNNNGGC 1 cut(s) 220
BglII AGATCT 1 cut(s) 159
BisI GCNGC 8 cut(s) 185, 224, 275, 316, 463, 480, 641, 700
BlsI GCNGC 8 cut(s) 186, 225, 276, 317, 464, 481, 642, 701
Bme1390I CCNGG 2 cut(s) 554, 725
Bme18I GGWCC 2 cut(s) 499, 711
BmgT120I GGNCC 3 cut(s) 499, 711, 767
BmiI GGNNCC 3 cut(s) 213, 466, 500
BmrFI CCNGG 2 cut(s) 554, 725
BpuMI CCSGG 1 cut(s) 725
BsaHI GRCGYC 1 cut(s) 398
BsaJI CCNNGG 2 cut(s) 694, 714
Bsc4I CCNNNNNNNGG 2 cut(s) 322, 356
Bse118I RCCGGY 1 cut(s) 214
Bse3DI GCAATG 1 cut(s) 339
BseBI CCWGG 1 cut(s) 554
BseDI CCNNGG 2 cut(s) 694, 714
BseGI GGATG 1 cut(s) 639
BseLI CCNNNNNNNGG 2 cut(s) 322, 356
BseMI GCAATG 1 cut(s) 339
BseMII CTCAG 1 cut(s) 177
BseRI GAGGAG 1 cut(s) 121
BseXI GCAGC 5 cut(s) 196, 210, 286, 491, 711
BsgI GTGCAG 1 cut(s) 596
Bsh1285I CGRYCG 1 cut(s) 420
BshFI GGCC 2 cut(s) 413, 769
BshNI GGYRCC 2 cut(s) 211, 464
BsiEI CGRYCG 1 cut(s) 420
BsiSI CCGG 2 cut(s) 215, 724
BslFI GGGAC 2 cut(s) 512, 732
BslI CCNNNNNNNGG 2 cut(s) 322, 356
BsmFI GGGAC 2 cut(s) 512, 732
BsmI GAATGC 1 cut(s) 606
BsnI GGCC 2 cut(s) 413, 769
Bsp143I GATC 2 cut(s) 159, 522
BspACI CCGC 7 cut(s) 19, 313, 316, 368, 420, 462, 641
BspANI GGCC 2 cut(s) 413, 769
BspCNI CTCAG 1 cut(s) 176
BspLI GGNNCC 3 cut(s) 213, 466, 500
BspT107I GGYRCC 2 cut(s) 211, 464
BsrDI GCAATG 1 cut(s) 339
BsrFI RCCGGY 1 cut(s) 214
BssAI RCCGGY 1 cut(s) 214
BssECI CCNNGG 2 cut(s) 694, 714
BssMI GATC 2 cut(s) 159, 522
BssNI GRCGYC 1 cut(s) 398
BssT1I CCWWGG 1 cut(s) 714
Bst2UI CCWGG 1 cut(s) 554
Bst4CI ACNGT 2 cut(s) 289, 688
Bst6I CTCTTC 1 cut(s) 572
BstACI GRCGYC 1 cut(s) 398
BstC8I GCNNGC 2 cut(s) 216, 604
BstDEI CTNAG 1 cut(s) 163
BstF5I GGATG 1 cut(s) 639
BstKTI GATC 2 cut(s) 162, 525
BstMBI GATC 2 cut(s) 159, 522
BstMCI CGRYCG 1 cut(s) 420
BstMWI GCNNNNNNNGC 4 cut(s) 220, 419, 603, 649
BstNI CCWGG 1 cut(s) 554
BstSCI CCNGG 2 cut(s) 552, 723
BstSFI CTRYAG 2 cut(s) 592, 666
BstV1I GCAGC 5 cut(s) 196, 210, 286, 491, 711
BstX2I RGATCY 1 cut(s) 159
BstYI RGATCY 1 cut(s) 159
BsuRI GGCC 2 cut(s) 413, 769
BtsCI GGATG 1 cut(s) 639
BtsI GCAGTG 1 cut(s) 441
BtsIMutI CAGTG 1 cut(s) 441
Cac8I GCNNGC 2 cut(s) 216, 604
Cfr10I RCCGGY 1 cut(s) 214
Cfr13I GGNCC 3 cut(s) 499, 711, 767
CseI GACGC 2 cut(s) 280, 387
Csp6I GTAC 2 cut(s) 41, 589
CviAII CATG 2 cut(s) 356, 624
CviQI GTAC 2 cut(s) 41, 589
DdeI CTNAG 1 cut(s) 163
DpnI GATC 2 cut(s) 161, 524
DpnII GATC 2 cut(s) 159, 522
Eam1104I CTCTTC 1 cut(s) 572
EarI CTCTTC 1 cut(s) 572
Eco130I CCWWGG 1 cut(s) 714
Eco47I GGWCC 2 cut(s) 499, 711
EcoRII CCWGG 1 cut(s) 552
EcoT14I CCWWGG 1 cut(s) 714
ErhI CCWWGG 1 cut(s) 714
FaeI CATG 2 cut(s) 359, 627
FaiI YATR 7 cut(s) 241, 250, 357, 392, 584, 625, 757
FaqI GGGAC 2 cut(s) 512, 732
FatI CATG 2 cut(s) 355, 623
FauI CCCGC 1 cut(s) 26
FblI GTMKAC 1 cut(s) 401
Fnu4HI GCNGC 8 cut(s) 185, 224, 275, 316, 463, 480, 641, 700
FokI GGATG 1 cut(s) 646
Fsp4HI GCNGC 8 cut(s) 185, 224, 275, 316, 463, 480, 641, 700
FspBI CTAG 2 cut(s) 821, 892
GluI GCNGC 8 cut(s) 185, 224, 275, 316, 463, 480, 641, 700
HaeIII GGCC 2 cut(s) 413, 769
HapII CCGG 2 cut(s) 215, 724
HgaI GACGC 2 cut(s) 280, 387
Hin1I GRCGYC 1 cut(s) 398
Hin1II CATG 2 cut(s) 359, 627
HincII GTYRAC 1 cut(s) 402
HindII GTYRAC 1 cut(s) 402
HinfI GANTC 5 cut(s) 57, 120, 264, 297, 720
HpaII CCGG 2 cut(s) 215, 724
Hpy166II GTNNAC 4 cut(s) 61, 328, 402, 691
Hpy188I TCNGA 4 cut(s) 150, 166, 296, 777
Hpy188III TCNNGA 2 cut(s) 268, 451
Hpy8I GTNNAC 4 cut(s) 61, 328, 402, 691
Hpy99I CGWCG 1 cut(s) 403
HpyCH4III ACNGT 2 cut(s) 289, 688
HpyCH4IV ACGT 2 cut(s) 204, 675
HpyCH4V TGCA 6 cut(s) 8, 184, 373, 515, 577, 627
HpyF10VI GCNNNNNNNGC 4 cut(s) 220, 419, 603, 649
HpyF3I CTNAG 1 cut(s) 163
HpySE526I ACGT 2 cut(s) 204, 675
Hsp92I GRCGYC 1 cut(s) 398
Hsp92II CATG 2 cut(s) 359, 627
KroI GCCGGC 1 cut(s) 214
KroNI GCCGGC 1 cut(s) 216
Kzo9I GATC 2 cut(s) 159, 522
LmnI GCTCC 1 cut(s) 352
Lsp1109I GCAGC 5 cut(s) 196, 210, 286, 491, 711
MaeI CTAG 2 cut(s) 821, 892
MaeII ACGT 2 cut(s) 204, 675
MaeIII GTNAC 1 cut(s) 109
MalI GATC 2 cut(s) 161, 524
MboI GATC 2 cut(s) 159, 522
MboII GAAGA 6 cut(s) 179, 182, 185, 188, 523, 559
MflI RGATCY 1 cut(s) 159
MluCI AATT 3 cut(s) 143, 301, 771
MlyI GAGTC 3 cut(s) 66, 114, 714
MroNI GCCGGC 1 cut(s) 214
MroXI GAANNNNTTC 2 cut(s) 144, 301
MslI CAYNNNNRTG 3 cut(s) 105, 542, 686
MspA1I CMGCKG 1 cut(s) 462
MspI CCGG 2 cut(s) 215, 724
MspR9I CCNGG 2 cut(s) 554, 725
Mva1269I GAATGC 1 cut(s) 606
MvaI CCWGG 1 cut(s) 554
MwoI GCNNNNNNNGC 4 cut(s) 220, 419, 603, 649
NaeI GCCGGC 1 cut(s) 216
NciI CCSGG 1 cut(s) 725
NdeII GATC 2 cut(s) 159, 522
NgoMIV GCCGGC 1 cut(s) 214
NlaIII CATG 2 cut(s) 359, 627
NlaIV GGNNCC 3 cut(s) 213, 466, 500
OliI CACNNNNGTG 2 cut(s) 105, 686
PctI GAATGC 1 cut(s) 606
PdiI GCCGGC 1 cut(s) 216
PdmI GAANNNNTTC 2 cut(s) 144, 301
PfeI GAWTC 2 cut(s) 264, 297
PflMI CCANNNNNTGG 1 cut(s) 356
PkrI GCNGC 8 cut(s) 186, 225, 276, 317, 464, 481, 642, 701
PleI GAGTC 3 cut(s) 65, 114, 714
PpsI GAGTC 3 cut(s) 65, 114, 714
Psp6I CCWGG 1 cut(s) 552
PspGI CCWGG 1 cut(s) 552
PspN4I GGNNCC 3 cut(s) 213, 466, 500
PspPI GGNCC 3 cut(s) 499, 711, 767
PsuI RGATCY 1 cut(s) 159
RsaI GTAC 2 cut(s) 42, 590
RsaNI GTAC 2 cut(s) 41, 589
RseI CAYNNNNRTG 3 cut(s) 105, 542, 686
SalI GTCGAC 1 cut(s) 400
SatI GCNGC 8 cut(s) 185, 224, 275, 316, 463, 480, 641, 700
Sau3AI GATC 2 cut(s) 159, 522
Sau96I GGNCC 3 cut(s) 499, 711, 767
SchI GAGTC 3 cut(s) 66, 114, 714
ScrFI CCNGG 2 cut(s) 554, 725
SfcI CTRYAG 2 cut(s) 592, 666
SinI GGWCC 2 cut(s) 499, 711
SmiMI CAYNNNNRTG 3 cut(s) 105, 542, 686
Sse9I AATT 3 cut(s) 143, 301, 771
SsiI CCGC 7 cut(s) 19, 313, 316, 368, 420, 462, 641
SspMI CTAG 2 cut(s) 821, 892
StyD4I CCNGG 2 cut(s) 552, 723
StyI CCWWGG 1 cut(s) 714
TaaI ACNGT 2 cut(s) 289, 688
TaiI ACGT 2 cut(s) 207, 678
TaqI TCGA 3 cut(s) 262, 401, 416
TaqII GACCGA 1 cut(s) 516
TasI AATT 3 cut(s) 143, 301, 771
TauI GCSGC 3 cut(s) 318, 465, 643
TfiI GAWTC 2 cut(s) 264, 297
TscAI CASTG 1 cut(s) 448
TseI GCWGC 5 cut(s) 184, 223, 274, 479, 699
TspDTI ATGAA 1 cut(s) 470
TspRI CASTG 1 cut(s) 448
Van91I CCANNNNNTGG 1 cut(s) 356
VpaK11BI GGWCC 2 cut(s) 499, 711
XmiI GTMKAC 1 cut(s) 401
XmnI GAANNNNTTC 2 cut(s) 144, 301
XspI CTAG 2 cut(s) 821, 892
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.