Rh1DG153900

Protein of unknown function, DUF599

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0007447
Physical Location & Seq
Forward (+)
32179061 .. 32179728
668 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG153900.1

Sequence Viewer

Length: 579 bp
ATGATGGAGGACACGTCCAAAAATGGTGTTCTTGCGGTGCAGACGCTTCGAAACAACATAATGGCATCAACCCTTTTGGCCTCCACGGCGATCATGCTCAGCTCTCTCATTGCTGTGTTGATGAGTAGTGTAAACAAAGATAGAAGTGCATCGGTTTTCGTTTACGGGAACACAAGCGAGAAGGGATACTCCATCAAATACTTTTCCATTTTGGTCTGTTTCTTGGTGGCTTTCTTGTTCAATGTGCAGTCCATAAGGTACTACAGTCATGCAAGCATTCTGATCAACGCGCCGTTCAAGAAGATGTCTCCGCATCTGAAGCATCACAATTTGACAACAGAGTATGTGGCAAACACTGTGAACAGAGGCAGCTACTTCTGGTCTTTGGGTCTCCGAGCCTTCTACTTTTCGTTCCCTCTGTTCTTGTGGATCTTTGGTCCCATACCGATGTTCTTGTCCTGCTTTGTTTTGGTTGTCATGCTCTATTTCCTTGACGTCACTTTCCAATTTGGGTGGGTTGTTGGGGTTGACGATGAGGAACATAGCATCATCACCAAGGATGAAGAACAAGGGGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF599

Protein Analysis

192

Amino Acids

21.78

Weight (kDa)

6.96

Isoelectric Point (pI)

41.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF599 PF04654 1 - 168 1.9e-59 Protein of unknown function, DUF599
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 498
AccII CGCG 1 cut(s) 290
AciI CCGC 2 cut(s) 35, 311
AclWI GGATC 1 cut(s) 437
AcuI CTGAAG 1 cut(s) 338
AcyI GRCGYC 1 cut(s) 495
AfaI GTAC 1 cut(s) 260
AflIII ACRYGT 1 cut(s) 12
AgsI TTSAA 2 cut(s) 241, 298
AjiI CACGTC 1 cut(s) 15
AluBI AGCT 2 cut(s) 102, 372
AluI AGCT 2 cut(s) 102, 372
Alw26I GTCTC 2 cut(s) 312, 395
AlwI GGATC 1 cut(s) 437
AoxI GGCC 1 cut(s) 78
ApeKI GCWGC 1 cut(s) 369
AspLEI GCGC 1 cut(s) 292
AspS9I GGNCC 1 cut(s) 437
AsuHPI GGTGA 1 cut(s) 544
AsuII TTCGAA 1 cut(s) 49
AvaII GGWCC 1 cut(s) 437
BaeI ACNNNNGTAYC 2 cut(s) 250, 283
BbvI GCAGC 1 cut(s) 381
BccI CCATC 1 cut(s) 200
BceAI ACGGC 2 cut(s) 102, 277
BcgI CGANNNNNNTGC 2 cut(s) 29, 63
BciVI GTATCC 1 cut(s) 179
BclI TGATCA 1 cut(s) 282
BcoDI GTCTC 2 cut(s) 312, 395
BfmI CTRYAG 1 cut(s) 262
BfuI GTATCC 1 cut(s) 179
BglI GCCNNNNNGGC 1 cut(s) 86
BisI GCNGC 1 cut(s) 370
BlpI GCTNAGC 1 cut(s) 98
BlsI GCNGC 1 cut(s) 371
Bme18I GGWCC 1 cut(s) 437
BmgBI CACGTC 1 cut(s) 15
BmgT120I GGNCC 1 cut(s) 437
BmiI GGNNCC 1 cut(s) 439
BmsI GCATC 5 cut(s) 74, 158, 322, 331, 555
Bpu1102I GCTNAGC 1 cut(s) 98
Bpu14I TTCGAA 1 cut(s) 49
BsaHI GRCGYC 1 cut(s) 495
BsaI GGTCTC 1 cut(s) 395
BsaJI CCNNGG 2 cut(s) 84, 555
BsaXI ACNNNNNCTCC 1 cut(s) 29
Bse3DI GCAATG 1 cut(s) 108
BseDI CCNNGG 2 cut(s) 84, 555
BseGI GGATG 1 cut(s) 565
BseMI GCAATG 1 cut(s) 108
BseMII CTCAG 1 cut(s) 112
BseXI GCAGC 1 cut(s) 381
BsgI GTGCAG 2 cut(s) 59, 266
Bsh1236I CGCG 1 cut(s) 290
BshFI GGCC 1 cut(s) 80
BslFI GGGAC 1 cut(s) 423
BsmAI GTCTC 2 cut(s) 312, 395
BsmFI GGGAC 1 cut(s) 423
BsmI GAATGC 1 cut(s) 276
BsnI GGCC 1 cut(s) 80
Bso31I GGTCTC 1 cut(s) 395
Bsp119I TTCGAA 1 cut(s) 49
Bsp143I GATC 3 cut(s) 90, 282, 429
Bsp1720I GCTNAGC 1 cut(s) 98
BspACI CCGC 2 cut(s) 35, 311
BspANI GGCC 1 cut(s) 80
BspCNI CTCAG 1 cut(s) 111
BspFNI CGCG 1 cut(s) 290
BspLI GGNNCC 1 cut(s) 439
BspPI GGATC 1 cut(s) 437
BspT104I TTCGAA 1 cut(s) 49
BspTNI GGTCTC 1 cut(s) 395
BsrDI GCAATG 1 cut(s) 108
BssECI CCNNGG 2 cut(s) 84, 555
BssMI GATC 3 cut(s) 90, 282, 429
BssNI GRCGYC 1 cut(s) 495
BssT1I CCWWGG 1 cut(s) 555
Bst4CI ACNGT 2 cut(s) 266, 358
BstACI GRCGYC 1 cut(s) 495
BstBI TTCGAA 1 cut(s) 49
BstC8I GCNNGC 1 cut(s) 274
BstDEI CTNAG 1 cut(s) 98
BstDSI CCRYGG 1 cut(s) 84
BstF5I GGATG 1 cut(s) 565
BstFNI CGCG 1 cut(s) 290
BstHHI GCGC 1 cut(s) 292
BstKTI GATC 3 cut(s) 93, 285, 432
BstMAI GTCTC 2 cut(s) 312, 395
BstMBI GATC 3 cut(s) 90, 282, 429
BstMWI GCNNNNNNNGC 2 cut(s) 86, 319
BstSFI CTRYAG 1 cut(s) 262
BstUI CGCG 1 cut(s) 290
BstV1I GCAGC 1 cut(s) 381
BstX2I RGATCY 1 cut(s) 429
BstYI RGATCY 1 cut(s) 429
BsuI GTATCC 1 cut(s) 179
BsuRI GGCC 1 cut(s) 80
BtgI CCRYGG 1 cut(s) 84
BtrI CACGTC 1 cut(s) 15
BtsCI GGATG 1 cut(s) 565
BtsIMutI CAGTG 1 cut(s) 354
Cac8I GCNNGC 1 cut(s) 274
CfoI GCGC 1 cut(s) 292
Cfr13I GGNCC 1 cut(s) 437
CseI GACGC 1 cut(s) 52
Csp6I GTAC 1 cut(s) 259
CspCI CAANNNNNGTGG 2 cut(s) 494, 529
CviAII CATG 3 cut(s) 94, 269, 478
CviJI RGCY 5 cut(s) 80, 102, 230, 372, 398
CviKI_1 RGCY 5 cut(s) 80, 102, 230, 372, 398
CviQI GTAC 1 cut(s) 259
DdeI CTNAG 1 cut(s) 98
DpnI GATC 3 cut(s) 92, 284, 431
DpnII GATC 3 cut(s) 90, 282, 429
Eco130I CCWWGG 1 cut(s) 555
Eco31I GGTCTC 1 cut(s) 395
Eco47I GGWCC 1 cut(s) 437
Eco57I CTGAAG 1 cut(s) 338
EcoT14I CCWWGG 1 cut(s) 555
ErhI CCWWGG 1 cut(s) 555
FaeI CATG 3 cut(s) 97, 272, 481
FaiI YATR 8 cut(s) 59, 95, 254, 270, 345, 443, 479, 543
FaqI GGGAC 1 cut(s) 423
FatI CATG 3 cut(s) 93, 268, 477
FbaI TGATCA 1 cut(s) 282
Fnu4HI GCNGC 1 cut(s) 370
FokI GGATG 1 cut(s) 572
Fsp4HI GCNGC 1 cut(s) 370
GlaI GCGC 1 cut(s) 291
GluI GCNGC 1 cut(s) 370
HaeIII GGCC 1 cut(s) 80
HgaI GACGC 1 cut(s) 52
HhaI GCGC 1 cut(s) 292
Hin1I GRCGYC 1 cut(s) 495
Hin1II CATG 3 cut(s) 97, 272, 481
Hin6I GCGC 1 cut(s) 290
HinP1I GCGC 1 cut(s) 290
HincII GTYRAC 1 cut(s) 529
HindII GTYRAC 1 cut(s) 529
HphI GGTGA 1 cut(s) 544
Hpy166II GTNNAC 4 cut(s) 133, 163, 361, 529
Hpy188I TCNGA 3 cut(s) 282, 318, 395
Hpy188III TCNNGA 1 cut(s) 298
Hpy8I GTNNAC 4 cut(s) 133, 163, 361, 529
HpyAV CCTTC 2 cut(s) 175, 409
HpyCH4III ACNGT 2 cut(s) 266, 358
HpyCH4IV ACGT 2 cut(s) 14, 495
HpyCH4V TGCA 4 cut(s) 40, 149, 247, 272
HpyF10VI GCNNNNNNNGC 2 cut(s) 86, 319
HpyF3I CTNAG 1 cut(s) 98
HpySE526I ACGT 2 cut(s) 14, 495
Hsp92I GRCGYC 1 cut(s) 495
Hsp92II CATG 3 cut(s) 97, 272, 481
HspAI GCGC 1 cut(s) 290
Ksp22I TGATCA 1 cut(s) 282
Kzo9I GATC 3 cut(s) 90, 282, 429
LpnPI CCDG 2 cut(s) 364, 472
Lsp1109I GCAGC 1 cut(s) 381
LweI GCATC 5 cut(s) 74, 158, 322, 331, 555
MaeII ACGT 2 cut(s) 14, 495
MaeIII GTNAC 1 cut(s) 496
MalI GATC 3 cut(s) 92, 284, 431
MboI GATC 3 cut(s) 90, 282, 429
MboII GAAGA 2 cut(s) 313, 575
MflI RGATCY 1 cut(s) 429
MluCI AATT 2 cut(s) 328, 506
MnlI CCTC 4 cut(s) 91, 359, 426, 529
MslI CAYNNNNRTG 2 cut(s) 113, 446
Mva1269I GAATGC 1 cut(s) 276
MvnI CGCG 1 cut(s) 290
MwoI GCNNNNNNNGC 2 cut(s) 86, 319
NdeII GATC 3 cut(s) 90, 282, 429
NlaIII CATG 3 cut(s) 97, 272, 481
NlaIV GGNNCC 1 cut(s) 439
NmuCI GTSAC 1 cut(s) 496
NspV TTCGAA 1 cut(s) 49
PctI GAATGC 1 cut(s) 276
PkrI GCNGC 1 cut(s) 371
PspN4I GGNNCC 1 cut(s) 439
PspPI GGNCC 1 cut(s) 437
PsuI RGATCY 1 cut(s) 429
RsaI GTAC 1 cut(s) 260
RsaNI GTAC 1 cut(s) 259
RseI CAYNNNNRTG 2 cut(s) 113, 446
SatI GCNGC 1 cut(s) 370
Sau3AI GATC 3 cut(s) 90, 282, 429
Sau96I GGNCC 1 cut(s) 437
SetI ASST 5 cut(s) 17, 104, 260, 374, 498
SfaNI GCATC 5 cut(s) 74, 158, 322, 331, 555
SfcI CTRYAG 1 cut(s) 262
SfuI TTCGAA 1 cut(s) 49
SinI GGWCC 1 cut(s) 437
SmiMI CAYNNNNRTG 2 cut(s) 113, 446
Sse9I AATT 2 cut(s) 328, 506
SsiI CCGC 2 cut(s) 35, 311
StyI CCWWGG 1 cut(s) 555
TaaI ACNGT 2 cut(s) 266, 358
TaiI ACGT 2 cut(s) 17, 498
TaqI TCGA 1 cut(s) 49
TasI AATT 2 cut(s) 328, 506
TscAI CASTG 1 cut(s) 361
TseFI GTSAC 1 cut(s) 496
TseI GCWGC 1 cut(s) 369
Tsp45I GTSAC 1 cut(s) 496
TspDTI ATGAA 1 cut(s) 576
TspRI CASTG 1 cut(s) 361
VpaK11BI GGWCC 1 cut(s) 437
ZraI GACGTC 1 cut(s) 496
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.