FvH4_7g33281

Protein of unknown function, DUF599

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0007447
Physical Location & Seq
Reverse (-)
23701234 .. 23703185
1952 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g33281.t1

Sequence Viewer

Length: 717 bp
ATGGAGAAAAGAGATATTGATTACGTTTTGGTCCCAGCAGGCCTCCTAGTCATGGTGGGATACCATCTCTGGCTCCTCTACCGAATCAAAACCCAACCCAACAGCACCGTCATCGGCATCAATTGCATCATTCGCCGTTTCTGGGTCGCCGCCATGATGGAGGACTCATCCAAAAATGGCGTTCTTGCGGTGCAGACGCTCCGAAACAACATAATGGCGTCTACCCTTTTAGCCTCGACGGCGATCATGCTCAGCTCTCTCATTGCTGTGTTGATGACTAGCGCAAACAAAGATCGAAGCGCGTCTGCTTTCGTTTTCGGGGACACAAGTCCGATGGGATACTCGATCAAGTACTTTGCCATTCTGGTGTGTTTCTTGGTGGCTTTCTTGTTCAATGTGCAGTCTATAAGGTATTACAGCCATGCAAGCATTCTGATCAACGTGCCCTTCAAGAAGATGTCACCGCATCTGAAGCATCACCATTTGACAACAGAGTACGTGGCGAACACTGTGAACAGAGGCAGCTATTTCTGGTCTTTGGGTCTCAGAGCTTTTTACTTCTCTTTCCCTCTCTTCTTGTGGATCTTTGGTCCCTTACCGATGTTATTTTGTTGCTTTATTTTGGTTACCATGCTGTACTTCCTTGACATCACTTTCCAGTTTGGGTGGGTTGCTGGGGTTGATGATGAGGATCAAAGCATCGTCATCGAGGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF599

Protein Analysis

239

Amino Acids

27.04

Weight (kDa)

8.65

Isoelectric Point (pI)

42.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF599 PF04654 9 - 219 1e-76 Protein of unknown function, DUF599
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 221
AccII CGCG 1 cut(s) 302
AciI CCGC 3 cut(s) 150, 188, 464
AclWI GGATC 2 cut(s) 590, 699
AcuI CTGAAG 1 cut(s) 491
AcyI GRCGYC 1 cut(s) 218
AfaI GTAC 3 cut(s) 353, 497, 638
AfiI CCNNNNNNNGG 2 cut(s) 52, 142
AgsI TTSAA 2 cut(s) 394, 451
AluBI AGCT 3 cut(s) 255, 525, 551
AluI AGCT 3 cut(s) 255, 525, 551
Alw26I GTCTC 1 cut(s) 548
AlwI GGATC 2 cut(s) 590, 699
AoxI GGCC 1 cut(s) 40
ApeKI GCWGC 1 cut(s) 522
AspLEI GCGC 2 cut(s) 284, 302
AspS9I GGNCC 2 cut(s) 31, 590
AsuHPI GGTGA 2 cut(s) 453, 470
AvaII GGWCC 2 cut(s) 31, 590
BaeGI GKGCMC 1 cut(s) 447
BbvI GCAGC 1 cut(s) 534
BccI CCATC 3 cut(s) 72, 151, 328
BceAI ACGGC 2 cut(s) 120, 255
BcgI CGANNNNNNTGC 3 cut(s) 94, 128, 688
BciVI GTATCC 2 cut(s) 53, 332
BclI TGATCA 1 cut(s) 435
BcoDI GTCTC 1 cut(s) 548
BfaI CTAG 2 cut(s) 47, 279
BfuI GTATCC 2 cut(s) 53, 332
BglI GCCNNNNNGGC 1 cut(s) 239
BisI GCNGC 2 cut(s) 150, 523
BlpI GCTNAGC 1 cut(s) 251
BlsI GCNGC 2 cut(s) 151, 524
BmcAI AGTACT 1 cut(s) 353
Bme18I GGWCC 2 cut(s) 31, 590
BmgT120I GGNCC 2 cut(s) 31, 590
BmiI GGNNCC 3 cut(s) 33, 74, 592
BmsI GCATC 5 cut(s) 126, 135, 475, 484, 708
BoxI GACNNNNGTC 1 cut(s) 327
Bpu1102I GCTNAGC 1 cut(s) 251
BsaAI YACGTR 1 cut(s) 499
BsaBI GATNNNNATC 1 cut(s) 690
BsaHI GRCGYC 1 cut(s) 218
BsaI GGTCTC 1 cut(s) 548
Bsc4I CCNNNNNNNGG 2 cut(s) 52, 142
Bse1I ACTGG 1 cut(s) 658
Bse3DI GCAATG 1 cut(s) 261
Bse8I GATNNNNATC 1 cut(s) 690
BseGI GGATG 1 cut(s) 167
BseJI GATNNNNATC 1 cut(s) 690
BseLI CCNNNNNNNGG 2 cut(s) 52, 142
BseMI GCAATG 1 cut(s) 261
BseMII CTCAG 2 cut(s) 265, 559
BseNI ACTGG 1 cut(s) 658
BseRI GAGGAG 1 cut(s) 65
BseSI GKGCMC 1 cut(s) 447
BseXI GCAGC 1 cut(s) 534
BseYI CCCAGC 2 cut(s) 34, 674
BsgI GTGCAG 2 cut(s) 212, 419
Bsh1236I CGCG 1 cut(s) 302
BshFI GGCC 1 cut(s) 42
BslFI GGGAC 3 cut(s) 17, 335, 576
BslI CCNNNNNNNGG 2 cut(s) 52, 142
BsmAI GTCTC 1 cut(s) 548
BsmFI GGGAC 3 cut(s) 17, 335, 576
BsmI GAATGC 1 cut(s) 429
BsnI GGCC 1 cut(s) 42
Bso31I GGTCTC 1 cut(s) 548
Bsp1286I GDGCHC 1 cut(s) 447
Bsp143I GATC 6 cut(s) 243, 292, 345, 435, 582, 691
Bsp1720I GCTNAGC 1 cut(s) 251
BspACI CCGC 3 cut(s) 150, 188, 464
BspANI GGCC 1 cut(s) 42
BspCNI CTCAG 2 cut(s) 264, 558
BspFNI CGCG 1 cut(s) 302
BspLI GGNNCC 3 cut(s) 33, 74, 592
BspPI GGATC 2 cut(s) 590, 699
BspTNI GGTCTC 1 cut(s) 548
BsrDI GCAATG 1 cut(s) 261
BsrI ACTGG 1 cut(s) 658
BssMI GATC 6 cut(s) 243, 292, 345, 435, 582, 691
BssNI GRCGYC 1 cut(s) 218
Bst4CI ACNGT 2 cut(s) 109, 511
Bst6I CTCTTC 1 cut(s) 578
BstACI GRCGYC 1 cut(s) 218
BstAPI GCANNNNNTGC 1 cut(s) 123
BstBAI YACGTR 1 cut(s) 499
BstC8I GCNNGC 2 cut(s) 40, 427
BstDEI CTNAG 2 cut(s) 251, 545
BstEII GGTNACC 1 cut(s) 625
BstF5I GGATG 1 cut(s) 167
BstFNI CGCG 1 cut(s) 302
BstHHI GCGC 2 cut(s) 284, 302
BstKTI GATC 6 cut(s) 246, 295, 348, 438, 585, 694
BstMAI GTCTC 1 cut(s) 548
BstMBI GATC 6 cut(s) 243, 292, 345, 435, 582, 691
BstMWI GCNNNNNNNGC 5 cut(s) 123, 132, 239, 426, 472
BstPAI GACNNNNGTC 1 cut(s) 327
BstPI GGTNACC 1 cut(s) 625
BstSLI GKGCMC 1 cut(s) 447
BstUI CGCG 1 cut(s) 302
BstV1I GCAGC 1 cut(s) 534
BstX2I RGATCY 1 cut(s) 582
BstYI RGATCY 1 cut(s) 582
BsuI GTATCC 2 cut(s) 53, 332
BsuRI GGCC 1 cut(s) 42
BtsCI GGATG 1 cut(s) 167
BtsIMutI CAGTG 1 cut(s) 507
Cac8I GCNNGC 2 cut(s) 40, 427
CfoI GCGC 2 cut(s) 284, 302
Cfr13I GGNCC 2 cut(s) 31, 590
CseI GACGC 3 cut(s) 205, 207, 291
Csp6I GTAC 3 cut(s) 352, 496, 637
CviAII CATG 5 cut(s) 52, 154, 247, 422, 631
CviJI RGCY 8 cut(s) 42, 73, 233, 255, 383, 420, 525, 551
CviKI_1 RGCY 8 cut(s) 42, 73, 233, 255, 383, 420, 525, 551
CviQI GTAC 3 cut(s) 352, 496, 637
DdeI CTNAG 2 cut(s) 251, 545
DpnI GATC 6 cut(s) 245, 294, 347, 437, 584, 693
DpnII GATC 6 cut(s) 243, 292, 345, 435, 582, 691
Eam1104I CTCTTC 1 cut(s) 578
EarI CTCTTC 1 cut(s) 578
Eco147I AGGCCT 1 cut(s) 42
Eco31I GGTCTC 1 cut(s) 548
Eco47I GGWCC 2 cut(s) 31, 590
Eco57I CTGAAG 1 cut(s) 491
Eco91I GGTNACC 1 cut(s) 625
EcoO65I GGTNACC 1 cut(s) 625
FaeI CATG 5 cut(s) 55, 157, 250, 425, 634
FaiI YATR 7 cut(s) 53, 155, 212, 248, 407, 423, 632
FaqI GGGAC 3 cut(s) 17, 335, 576
FatI CATG 5 cut(s) 51, 153, 246, 421, 630
FbaI TGATCA 1 cut(s) 435
FblI GTMKAC 1 cut(s) 221
Fnu4HI GCNGC 2 cut(s) 150, 523
FokI GGATG 1 cut(s) 154
Fsp4HI GCNGC 2 cut(s) 150, 523
FspBI CTAG 2 cut(s) 47, 279
GlaI GCGC 2 cut(s) 283, 301
GluI GCNGC 2 cut(s) 150, 523
GsaI CCCAGC 2 cut(s) 38, 678
HaeIII GGCC 1 cut(s) 42
HgaI GACGC 3 cut(s) 205, 207, 291
HhaI GCGC 2 cut(s) 284, 302
Hin1I GRCGYC 1 cut(s) 218
Hin1II CATG 5 cut(s) 55, 157, 250, 425, 634
Hin6I GCGC 2 cut(s) 282, 300
HinP1I GCGC 2 cut(s) 282, 300
HinfI GANTC 2 cut(s) 84, 164
HphI GGTGA 2 cut(s) 453, 470
Hpy166II GTNNAC 2 cut(s) 222, 514
Hpy188I TCNGA 5 cut(s) 203, 333, 435, 471, 548
Hpy188III TCNNGA 1 cut(s) 451
Hpy8I GTNNAC 2 cut(s) 222, 514
Hpy99I CGWCG 1 cut(s) 241
HpyAV CCTTC 1 cut(s) 457
HpyCH4III ACNGT 2 cut(s) 109, 511
HpyCH4IV ACGT 3 cut(s) 24, 441, 498
HpyCH4V TGCA 4 cut(s) 126, 193, 400, 425
HpyF10VI GCNNNNNNNGC 5 cut(s) 123, 132, 239, 426, 472
HpyF3I CTNAG 2 cut(s) 251, 545
HpySE526I ACGT 3 cut(s) 24, 441, 498
Hsp92I GRCGYC 1 cut(s) 218
Hsp92II CATG 5 cut(s) 55, 157, 250, 425, 634
HspAI GCGC 2 cut(s) 282, 300
Ksp22I TGATCA 1 cut(s) 435
Kzo9I GATC 6 cut(s) 243, 292, 345, 435, 582, 691
LmnI GCTCC 2 cut(s) 78, 204
LpnPI CCDG 8 cut(s) 24, 48, 55, 127, 350, 517, 660, 671
Lsp1109I GCAGC 1 cut(s) 534
LweI GCATC 5 cut(s) 126, 135, 475, 484, 708
MaeI CTAG 2 cut(s) 47, 279
MaeII ACGT 3 cut(s) 24, 441, 498
MaeIII GTNAC 2 cut(s) 459, 625
MalI GATC 6 cut(s) 245, 294, 347, 437, 584, 693
MboI GATC 6 cut(s) 243, 292, 345, 435, 582, 691
MboII GAAGA 2 cut(s) 466, 565
MfeI CAATTG 1 cut(s) 121
MflI RGATCY 1 cut(s) 582
MhlI GDGCHC 1 cut(s) 447
MluCI AATT 1 cut(s) 121
MlyI GAGTC 1 cut(s) 158
MnlI CCTC 8 cut(s) 53, 86, 154, 244, 512, 579, 682, 703
MslI CAYNNNNRTG 2 cut(s) 266, 365
MunI CAATTG 1 cut(s) 121
Mva1269I GAATGC 1 cut(s) 429
MvnI CGCG 1 cut(s) 302
MwoI GCNNNNNNNGC 5 cut(s) 123, 132, 239, 426, 472
NdeII GATC 6 cut(s) 243, 292, 345, 435, 582, 691
NlaIII CATG 5 cut(s) 55, 157, 250, 425, 634
NlaIV GGNNCC 3 cut(s) 33, 74, 592
NmuCI GTSAC 1 cut(s) 459
PceI AGGCCT 1 cut(s) 42
PctI GAATGC 1 cut(s) 429
PfeI GAWTC 1 cut(s) 84
PkrI GCNGC 2 cut(s) 151, 524
PleI GAGTC 1 cut(s) 158
PpsI GAGTC 1 cut(s) 158
Ppu21I YACGTR 1 cut(s) 499
PshAI GACNNNNGTC 1 cut(s) 327
PspEI GGTNACC 1 cut(s) 625
PspFI CCCAGC 2 cut(s) 34, 674
PspN4I GGNNCC 3 cut(s) 33, 74, 592
PspPI GGNCC 2 cut(s) 31, 590
PsuI RGATCY 1 cut(s) 582
RsaI GTAC 3 cut(s) 353, 497, 638
RsaNI GTAC 3 cut(s) 352, 496, 637
RseI CAYNNNNRTG 2 cut(s) 266, 365
SatI GCNGC 2 cut(s) 150, 523
Sau3AI GATC 6 cut(s) 243, 292, 345, 435, 582, 691
Sau96I GGNCC 2 cut(s) 31, 590
ScaI AGTACT 1 cut(s) 353
SchI GAGTC 1 cut(s) 158
SduI GDGCHC 1 cut(s) 447
SetI ASST 7 cut(s) 27, 257, 413, 444, 501, 527, 553
SfaNI GCATC 5 cut(s) 126, 135, 475, 484, 708
SinI GGWCC 2 cut(s) 31, 590
SmiMI CAYNNNNRTG 2 cut(s) 266, 365
Sse9I AATT 1 cut(s) 121
SseBI AGGCCT 1 cut(s) 42
SsiI CCGC 3 cut(s) 150, 188, 464
SspMI CTAG 2 cut(s) 47, 279
StuI AGGCCT 1 cut(s) 42
TaaI ACNGT 2 cut(s) 109, 511
TaiI ACGT 3 cut(s) 27, 444, 501
TaqI TCGA 4 cut(s) 236, 295, 344, 708
TasI AATT 1 cut(s) 121
TatI WGTACW 2 cut(s) 351, 636
TauI GCSGC 1 cut(s) 152
TfiI GAWTC 1 cut(s) 84
TscAI CASTG 1 cut(s) 514
TseFI GTSAC 1 cut(s) 459
TseI GCWGC 1 cut(s) 522
Tsp45I GTSAC 1 cut(s) 459
TspRI CASTG 1 cut(s) 514
VpaK11BI GGWCC 2 cut(s) 31, 590
XmiI GTMKAC 1 cut(s) 221
XspI CTAG 2 cut(s) 47, 279
ZrmI AGTACT 1 cut(s) 353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.