MD07G1302500.v1.1

Protein of unknown function, DUF599

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0007447
Physical Location & Seq
Reverse (-)
36077929 .. 36079105
1177 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1302500.v1.1.491

Sequence Viewer

Length: 804 bp
ATGCAGCTAAAAGTTCTGGATTACATTTTGGTACCAGCAGGCCTCCTGCTGATGGCGGCTTACCATCTATGGCTCCTATGTCGAATCGTTAAGCAACCCAATAAGACCGTCATCGGCATCAACTCCATCAATCGCCGCTTCTGGGTTCAAGCCATGATGGACGATGCTCCAAAGAATGGTATTCTTCCGGTGCAGACGCTTCGAAACAACATAATGGCATCGACCGTTTTGGCCTCGACAGCGATCATGCTCTGCTCTCTCATTGCCCTCTTGATGACCAGCAGCAACAGGGGAGCATTTTTCGTTTTCGGAAACCGAAGTGAGTTTGCGTTTTCGATCAAGTTCTTCGCCATTTTAGTGTGTTTCTTGGTGGCATTCCTGTTCAACGTGCAGTCCATAAGATACTACAGCCATGCCAGCATGCTCATCAACGCGCCGTTCAAGAAGATGATGTCGCCGCCGCACCACAAGCAGCAGTACCTCACGCAAGAGTACGTTGCCACCACCGTGAACCGAGGCAGCTATTTCTGGTCCCTGGGACTCCGAGCATTCTACTTCTCGTTCCCTCTGTTTCTGTGGATTTTCGGGCCGATTTCGATGTTCTTGTCGTGCGTTGTTTTGGTGATCATGCTGTATTTTCTGGATTTCACCTTTCAGTCTAATTCGGTTGTTGGGGTTGCTTGGGATGATGACCCCATTCTCATTAAGGATGATGAGGAGCAAGGCTTGTCCAATGCACCGGGGATGACACATTGTGATGGTATCTCAAGTGTAGTGTGTGAGAAACCACGTCACAGTGCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF599

Protein Analysis

268

Amino Acids

30.23

Weight (kDa)

8.89

Isoelectric Point (pI)

45.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF599 PF04654 9 - 218 3.3e-75 Protein of unknown function, DUF599
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 31
AccB1I GGYRCC 1 cut(s) 31
AccB7I CCANNNNNTGG 1 cut(s) 176
AccII CGCG 1 cut(s) 434
AciI CCGC 4 cut(s) 56, 136, 458, 461
AdeI CACNNNGTG 1 cut(s) 755
AfaI GTAC 3 cut(s) 33, 479, 494
AfiI CCNNNNNNNGG 3 cut(s) 52, 142, 176
AgsI TTSAA 3 cut(s) 149, 385, 442
AjiI CACGTC 1 cut(s) 791
AjnI CCWGG 1 cut(s) 534
AleI CACNNNNGTG 1 cut(s) 506
AluBI AGCT 2 cut(s) 7, 522
AluI AGCT 2 cut(s) 7, 522
AoxI GGCC 3 cut(s) 40, 231, 587
ApeKI GCWGC 4 cut(s) 4, 282, 472, 519
Asp718I GGTACC 1 cut(s) 31
AspLEI GCGC 1 cut(s) 436
AspS9I GGNCC 2 cut(s) 531, 587
AsuC2I CCSGG 1 cut(s) 741
AsuHPI GGTGA 2 cut(s) 634, 640
AsuII TTCGAA 1 cut(s) 202
AvaII GGWCC 1 cut(s) 531
BanI GGYRCC 1 cut(s) 31
BbvI GCAGC 4 cut(s) 16, 294, 484, 531
BccI CCATC 5 cut(s) 46, 72, 134, 151, 752
BceAI ACGGC 1 cut(s) 421
BcgI CGANNNNNNTGC 2 cut(s) 182, 216
BciT130I CCWGG 1 cut(s) 536
BclI TGATCA 1 cut(s) 624
BcnI CCSGG 1 cut(s) 741
BfmI CTRYAG 1 cut(s) 406
BisI GCNGC 8 cut(s) 5, 57, 136, 283, 458, 461, 473, 520
BlsI GCNGC 8 cut(s) 6, 58, 137, 284, 459, 462, 474, 521
Bme1390I CCNGG 2 cut(s) 536, 741
Bme18I GGWCC 1 cut(s) 531
BmgBI CACGTC 1 cut(s) 791
BmgT120I GGNCC 2 cut(s) 531, 587
BmiI GGNNCC 3 cut(s) 33, 74, 533
BmrFI CCNGG 2 cut(s) 536, 741
BmsI GCATC 3 cut(s) 126, 154, 227
Bpu14I TTCGAA 1 cut(s) 202
BpuEI CTTGAG 1 cut(s) 751
BpuMI CCSGG 1 cut(s) 741
BsaJI CCNNGG 4 cut(s) 514, 534, 535, 740
BsaWI WCCGGW 1 cut(s) 187
Bsc4I CCNNNNNNNGG 3 cut(s) 52, 142, 176
Bse3DI GCAATG 1 cut(s) 261
BseBI CCWGG 1 cut(s) 536
BseDI CCNNGG 4 cut(s) 514, 534, 535, 740
BseGI GGATG 3 cut(s) 691, 715, 750
BseLI CCNNNNNNNGG 3 cut(s) 52, 142, 176
BseMI GCAATG 1 cut(s) 261
BseRI GAGGAG 1 cut(s) 731
BseXI GCAGC 4 cut(s) 16, 294, 484, 531
BsgI GTGCAG 2 cut(s) 212, 410
Bsh1236I CGCG 1 cut(s) 434
Bsh1285I CGRYCG 1 cut(s) 225
BshFI GGCC 3 cut(s) 42, 233, 589
BshNI GGYRCC 1 cut(s) 31
BsiEI CGRYCG 1 cut(s) 225
BsiSI CCGG 2 cut(s) 188, 740
BslFI GGGAC 2 cut(s) 517, 552
BslI CCNNNNNNNGG 3 cut(s) 52, 142, 176
BsmFI GGGAC 2 cut(s) 517, 552
BsmI GAATGC 2 cut(s) 374, 548
BsnI GGCC 3 cut(s) 42, 233, 589
Bsp119I TTCGAA 1 cut(s) 202
Bsp143I GATC 3 cut(s) 243, 336, 624
BspACI CCGC 4 cut(s) 56, 136, 458, 461
BspANI GGCC 3 cut(s) 42, 233, 589
BspFNI CGCG 1 cut(s) 434
BspLI GGNNCC 3 cut(s) 33, 74, 533
BspT104I TTCGAA 1 cut(s) 202
BspT107I GGYRCC 1 cut(s) 31
BsrDI GCAATG 1 cut(s) 261
BssECI CCNNGG 4 cut(s) 514, 534, 535, 740
BssMI GATC 3 cut(s) 243, 336, 624
Bst2UI CCWGG 1 cut(s) 536
Bst4CI ACNGT 4 cut(s) 109, 226, 508, 797
BstBI TTCGAA 1 cut(s) 202
BstC8I GCNNGC 3 cut(s) 40, 418, 422
BstF5I GGATG 3 cut(s) 691, 715, 750
BstFNI CGCG 1 cut(s) 434
BstHHI GCGC 1 cut(s) 436
BstKTI GATC 3 cut(s) 246, 339, 627
BstMBI GATC 3 cut(s) 243, 336, 624
BstMCI CGRYCG 1 cut(s) 225
BstMWI GCNNNNNNNGC 3 cut(s) 239, 417, 469
BstNI CCWGG 1 cut(s) 536
BstNSI RCATGY 1 cut(s) 424
BstSCI CCNGG 2 cut(s) 534, 739
BstSFI CTRYAG 1 cut(s) 406
BstUI CGCG 1 cut(s) 434
BstV1I GCAGC 4 cut(s) 16, 294, 484, 531
BsuRI GGCC 3 cut(s) 42, 233, 589
BtrI CACGTC 1 cut(s) 791
BtsCI GGATG 3 cut(s) 691, 715, 750
BtsIMutI CAGTG 1 cut(s) 802
Cac8I GCNNGC 3 cut(s) 40, 418, 422
CfoI GCGC 1 cut(s) 436
Cfr13I GGNCC 2 cut(s) 531, 587
CseI GACGC 1 cut(s) 205
Csp6I GTAC 3 cut(s) 32, 478, 493
CviAII CATG 5 cut(s) 154, 247, 413, 421, 628
CviQI GTAC 3 cut(s) 32, 478, 493
DpnI GATC 3 cut(s) 245, 338, 626
DpnII GATC 3 cut(s) 243, 336, 624
DraIII CACNNNGTG 1 cut(s) 755
Eco147I AGGCCT 1 cut(s) 42
Eco47I GGWCC 1 cut(s) 531
EcoRII CCWGG 1 cut(s) 534
FaeI CATG 5 cut(s) 157, 250, 416, 424, 631
FaiI YATR 9 cut(s) 70, 79, 155, 212, 248, 398, 414, 422, 629
FaqI GGGAC 2 cut(s) 517, 552
FatI CATG 5 cut(s) 153, 246, 412, 420, 627
FbaI TGATCA 1 cut(s) 624
Fnu4HI GCNGC 8 cut(s) 5, 57, 136, 283, 458, 461, 473, 520
FokI GGATG 3 cut(s) 698, 722, 757
Fsp4HI GCNGC 8 cut(s) 5, 57, 136, 283, 458, 461, 473, 520
GlaI GCGC 1 cut(s) 435
GluI GCNGC 8 cut(s) 5, 57, 136, 283, 458, 461, 473, 520
HaeIII GGCC 3 cut(s) 42, 233, 589
HapII CCGG 2 cut(s) 188, 740
HgaI GACGC 1 cut(s) 205
HhaI GCGC 1 cut(s) 436
Hin1II CATG 5 cut(s) 157, 250, 416, 424, 631
Hin6I GCGC 1 cut(s) 434
HinP1I GCGC 1 cut(s) 434
HinfI GANTC 2 cut(s) 84, 540
HpaII CCGG 2 cut(s) 188, 740
HphI GGTGA 2 cut(s) 634, 640
Hpy166II GTNNAC 1 cut(s) 511
Hpy188I TCNGA 2 cut(s) 311, 545
Hpy188III TCNNGA 4 cut(s) 17, 271, 442, 641
Hpy8I GTNNAC 1 cut(s) 511
HpyCH4III ACNGT 4 cut(s) 109, 226, 508, 797
HpyCH4IV ACGT 3 cut(s) 387, 495, 790
HpyCH4V TGCA 4 cut(s) 4, 193, 391, 737
HpyF10VI GCNNNNNNNGC 3 cut(s) 239, 417, 469
HpySE526I ACGT 3 cut(s) 387, 495, 790
Hsp92II CATG 5 cut(s) 157, 250, 416, 424, 631
HspAI GCGC 1 cut(s) 434
KpnI GGTACC 1 cut(s) 35
Ksp22I TGATCA 1 cut(s) 624
Kzo9I GATC 3 cut(s) 243, 336, 624
LmnI GCTCC 4 cut(s) 78, 172, 293, 718
Lsp1109I GCAGC 4 cut(s) 16, 294, 484, 531
LweI GCATC 3 cut(s) 126, 154, 227
MaeII ACGT 3 cut(s) 387, 495, 790
MaeIII GTNAC 1 cut(s) 791
MalI GATC 3 cut(s) 245, 338, 626
MboI GATC 3 cut(s) 243, 336, 624
MboII GAAGA 3 cut(s) 176, 337, 457
MluCI AATT 1 cut(s) 661
MlyI GAGTC 1 cut(s) 534
MnlI CCTC 7 cut(s) 53, 244, 278, 491, 509, 576, 709
MseI TTAA 2 cut(s) 90, 705
MslI CAYNNNNRTG 3 cut(s) 356, 506, 756
MspI CCGG 2 cut(s) 188, 740
MspR9I CCNGG 2 cut(s) 536, 741
Mva1269I GAATGC 2 cut(s) 374, 548
MvaI CCWGG 1 cut(s) 536
MvnI CGCG 1 cut(s) 434
MwoI GCNNNNNNNGC 3 cut(s) 239, 417, 469
NciI CCSGG 1 cut(s) 741
NdeII GATC 3 cut(s) 243, 336, 624
NlaIII CATG 5 cut(s) 157, 250, 416, 424, 631
NlaIV GGNNCC 3 cut(s) 33, 74, 533
NmuCI GTSAC 1 cut(s) 791
NspI RCATGY 1 cut(s) 424
NspV TTCGAA 1 cut(s) 202
OliI CACNNNNGTG 1 cut(s) 506
PaeI GCATGC 1 cut(s) 424
PasI CCCWGGG 1 cut(s) 535
PceI AGGCCT 1 cut(s) 42
PctI GAATGC 2 cut(s) 374, 548
PfeI GAWTC 1 cut(s) 84
PflMI CCANNNNNTGG 1 cut(s) 176
PkrI GCNGC 8 cut(s) 6, 58, 137, 284, 459, 462, 474, 521
PleI GAGTC 1 cut(s) 534
PpsI GAGTC 1 cut(s) 534
Psp6I CCWGG 1 cut(s) 534
PspGI CCWGG 1 cut(s) 534
PspN4I GGNNCC 3 cut(s) 33, 74, 533
PspPI GGNCC 2 cut(s) 531, 587
RsaI GTAC 3 cut(s) 33, 479, 494
RsaNI GTAC 3 cut(s) 32, 478, 493
RseI CAYNNNNRTG 3 cut(s) 356, 506, 756
SaqAI TTAA 2 cut(s) 90, 705
SatI GCNGC 8 cut(s) 5, 57, 136, 283, 458, 461, 473, 520
Sau3AI GATC 3 cut(s) 243, 336, 624
Sau96I GGNCC 2 cut(s) 531, 587
SchI GAGTC 1 cut(s) 534
ScrFI CCNGG 2 cut(s) 536, 741
SetI ASST 7 cut(s) 9, 390, 483, 498, 524, 653, 793
SfaNI GCATC 3 cut(s) 126, 154, 227
SfcI CTRYAG 1 cut(s) 406
SfuI TTCGAA 1 cut(s) 202
SinI GGWCC 1 cut(s) 531
SmiMI CAYNNNNRTG 3 cut(s) 356, 506, 756
SmlI CTYRAG 1 cut(s) 766
SmoI CTYRAG 1 cut(s) 766
SphI GCATGC 1 cut(s) 424
Sse9I AATT 1 cut(s) 661
SseBI AGGCCT 1 cut(s) 42
SsiI CCGC 4 cut(s) 56, 136, 458, 461
StuI AGGCCT 1 cut(s) 42
StyD4I CCNGG 2 cut(s) 534, 739
TaaI ACNGT 4 cut(s) 109, 226, 508, 797
TaiI ACGT 3 cut(s) 390, 498, 793
TaqI TCGA 6 cut(s) 82, 202, 221, 236, 335, 596
TasI AATT 1 cut(s) 661
TauI GCSGC 4 cut(s) 59, 138, 460, 463
TfiI GAWTC 1 cut(s) 84
Tru1I TTAA 2 cut(s) 90, 705
Tru9I TTAA 2 cut(s) 90, 705
TscAI CASTG 1 cut(s) 802
TseFI GTSAC 1 cut(s) 791
TseI GCWGC 4 cut(s) 4, 282, 472, 519
Tsp45I GTSAC 1 cut(s) 791
TspRI CASTG 1 cut(s) 802
Van91I CCANNNNNTGG 1 cut(s) 176
VpaK11BI GGWCC 1 cut(s) 531
XceI RCATGY 1 cut(s) 424
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.