FvH4_7g33270

Protein of unknown function, DUF599

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0007447
Physical Location & Seq
Reverse (-)
23698334 .. 23699132
799 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g33270.t1

Sequence Viewer

Length: 717 bp
ATGGAGAAAAGAGATATTGATTATGTTTTGGTCCCAGCAGGCCTCCTAGTCATGGTGGGATACCATCTCTGGCTCCTCTACCGAATCAAAACACAACCCAACAGCACCGTCATCGGCATCAACTGCATCAATCGCCGTTTCTGGGTCGCCGCCATGATGGAGGACTCATCCAAAAATGGCGTTCTTGCGGTGCAGACGCTCCGAAACAACATAATGGCGTCTACCCTTTTAGCCTCGACGGCGATTATGCTCAGCTCTCTCATTGCTGTGTTGATGACTAGCGGAAACAAAGATCGAACCGCGTCTGCTTTCGTTTTCGGGGACACAAGTCCGATGGGATACTCGATCAAGTACTTTGCCATTCTGGTGTGTTTCTTGGTGGCTTTCTTGTTCAATGTGCAGTCCATAAGATATTACAGTCATACAAGCATGCTGATCAACGTGCCCTTCAAGAAGATGTCACCGCATCTGAAGCATCACCATTTGACAACAGAGTACGTGGCGGACACTGTGAACAGAGGCAGCTATTTCTGGTCTTTGGGTCTCAGAGCTTTTTACTTCTCTTTCCCTCTCTTCTTGTGGATCTTTGGTCCCATACCGATGTTCTTTTGTTGCTTTATTTTGGTTACCATGCTGTACTTCCTTGACGTCACTTGCCAGTTTGGGTGGGTTGCTGGGGTTGATGATGAGGAACAAAGCATCGTCATCGAGGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF599

Protein Analysis

239

Amino Acids

27.12

Weight (kDa)

8.25

Isoelectric Point (pI)

37.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF599 PF04654 9 - 219 3e-77 Protein of unknown function, DUF599
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 651
AccI GTMKAC 1 cut(s) 221
AccII CGCG 1 cut(s) 302
AciI CCGC 6 cut(s) 150, 188, 282, 300, 464, 503
AclWI GGATC 1 cut(s) 590
AcuI CTGAAG 1 cut(s) 491
AcyI GRCGYC 2 cut(s) 218, 648
AfaI GTAC 3 cut(s) 353, 497, 638
AfiI CCNNNNNNNGG 2 cut(s) 52, 142
AgsI TTSAA 2 cut(s) 394, 451
AluBI AGCT 3 cut(s) 255, 525, 551
AluI AGCT 3 cut(s) 255, 525, 551
Alw26I GTCTC 1 cut(s) 548
AlwI GGATC 1 cut(s) 590
AoxI GGCC 1 cut(s) 40
ApeKI GCWGC 1 cut(s) 522
AspS9I GGNCC 2 cut(s) 31, 590
AsuHPI GGTGA 2 cut(s) 453, 470
AvaII GGWCC 2 cut(s) 31, 590
BaeGI GKGCMC 1 cut(s) 447
BbvI GCAGC 1 cut(s) 534
BccI CCATC 3 cut(s) 72, 151, 328
BceAI ACGGC 2 cut(s) 120, 255
BcgI CGANNNNNNTGC 3 cut(s) 94, 128, 688
BciVI GTATCC 2 cut(s) 53, 332
BclI TGATCA 1 cut(s) 435
BcoDI GTCTC 1 cut(s) 548
BfaI CTAG 2 cut(s) 47, 279
BfuI GTATCC 2 cut(s) 53, 332
BglI GCCNNNNNGGC 1 cut(s) 239
BisI GCNGC 2 cut(s) 150, 523
BlpI GCTNAGC 1 cut(s) 251
BlsI GCNGC 2 cut(s) 151, 524
BmcAI AGTACT 1 cut(s) 353
Bme18I GGWCC 2 cut(s) 31, 590
BmgT120I GGNCC 2 cut(s) 31, 590
BmiI GGNNCC 3 cut(s) 33, 74, 592
BmsI GCATC 5 cut(s) 126, 135, 475, 484, 708
BoxI GACNNNNGTC 1 cut(s) 327
Bpu1102I GCTNAGC 1 cut(s) 251
BsaAI YACGTR 1 cut(s) 499
BsaHI GRCGYC 2 cut(s) 218, 648
BsaI GGTCTC 1 cut(s) 548
Bsc4I CCNNNNNNNGG 2 cut(s) 52, 142
Bse1I ACTGG 1 cut(s) 658
Bse3DI GCAATG 1 cut(s) 261
BseGI GGATG 1 cut(s) 167
BseLI CCNNNNNNNGG 2 cut(s) 52, 142
BseMI GCAATG 1 cut(s) 261
BseMII CTCAG 2 cut(s) 265, 559
BseNI ACTGG 1 cut(s) 658
BseRI GAGGAG 1 cut(s) 65
BseSI GKGCMC 1 cut(s) 447
BseXI GCAGC 1 cut(s) 534
BseYI CCCAGC 2 cut(s) 34, 674
BsgI GTGCAG 2 cut(s) 212, 419
Bsh1236I CGCG 1 cut(s) 302
BshFI GGCC 1 cut(s) 42
BslFI GGGAC 3 cut(s) 17, 335, 576
BslI CCNNNNNNNGG 2 cut(s) 52, 142
BsmAI GTCTC 1 cut(s) 548
BsmFI GGGAC 3 cut(s) 17, 335, 576
BsnI GGCC 1 cut(s) 42
Bso31I GGTCTC 1 cut(s) 548
Bsp1286I GDGCHC 1 cut(s) 447
Bsp143I GATC 4 cut(s) 292, 345, 435, 582
Bsp1720I GCTNAGC 1 cut(s) 251
BspACI CCGC 6 cut(s) 150, 188, 282, 300, 464, 503
BspANI GGCC 1 cut(s) 42
BspCNI CTCAG 2 cut(s) 264, 558
BspFNI CGCG 1 cut(s) 302
BspLI GGNNCC 3 cut(s) 33, 74, 592
BspPI GGATC 1 cut(s) 590
BspTNI GGTCTC 1 cut(s) 548
BsrDI GCAATG 1 cut(s) 261
BsrI ACTGG 1 cut(s) 658
BssMI GATC 4 cut(s) 292, 345, 435, 582
BssNI GRCGYC 2 cut(s) 218, 648
Bst4CI ACNGT 3 cut(s) 109, 419, 511
Bst6I CTCTTC 1 cut(s) 578
BstACI GRCGYC 2 cut(s) 218, 648
BstAPI GCANNNNNTGC 1 cut(s) 123
BstBAI YACGTR 1 cut(s) 499
BstC8I GCNNGC 2 cut(s) 40, 431
BstDEI CTNAG 2 cut(s) 251, 545
BstEII GGTNACC 1 cut(s) 625
BstF5I GGATG 1 cut(s) 167
BstFNI CGCG 1 cut(s) 302
BstKTI GATC 4 cut(s) 295, 348, 438, 585
BstMAI GTCTC 1 cut(s) 548
BstMBI GATC 4 cut(s) 292, 345, 435, 582
BstMWI GCNNNNNNNGC 4 cut(s) 123, 132, 239, 472
BstNSI RCATGY 1 cut(s) 433
BstPAI GACNNNNGTC 1 cut(s) 327
BstPI GGTNACC 1 cut(s) 625
BstSLI GKGCMC 1 cut(s) 447
BstUI CGCG 1 cut(s) 302
BstV1I GCAGC 1 cut(s) 534
BstX2I RGATCY 1 cut(s) 582
BstYI RGATCY 1 cut(s) 582
BsuI GTATCC 2 cut(s) 53, 332
BsuRI GGCC 1 cut(s) 42
BtsCI GGATG 1 cut(s) 167
BtsIMutI CAGTG 1 cut(s) 507
Cac8I GCNNGC 2 cut(s) 40, 431
Cfr13I GGNCC 2 cut(s) 31, 590
CseI GACGC 3 cut(s) 205, 207, 291
Csp6I GTAC 3 cut(s) 352, 496, 637
CviAII CATG 4 cut(s) 52, 154, 430, 631
CviJI RGCY 7 cut(s) 42, 73, 233, 255, 383, 525, 551
CviKI_1 RGCY 7 cut(s) 42, 73, 233, 255, 383, 525, 551
CviQI GTAC 3 cut(s) 352, 496, 637
DdeI CTNAG 2 cut(s) 251, 545
DpnI GATC 4 cut(s) 294, 347, 437, 584
DpnII GATC 4 cut(s) 292, 345, 435, 582
Eam1104I CTCTTC 1 cut(s) 578
EarI CTCTTC 1 cut(s) 578
EciI GGCGGA 1 cut(s) 518
Eco147I AGGCCT 1 cut(s) 42
Eco31I GGTCTC 1 cut(s) 548
Eco47I GGWCC 2 cut(s) 31, 590
Eco57I CTGAAG 1 cut(s) 491
Eco91I GGTNACC 1 cut(s) 625
EcoO65I GGTNACC 1 cut(s) 625
FaeI CATG 4 cut(s) 55, 157, 433, 634
FaqI GGGAC 3 cut(s) 17, 335, 576
FatI CATG 4 cut(s) 51, 153, 429, 630
FbaI TGATCA 1 cut(s) 435
FblI GTMKAC 1 cut(s) 221
Fnu4HI GCNGC 2 cut(s) 150, 523
FokI GGATG 1 cut(s) 154
Fsp4HI GCNGC 2 cut(s) 150, 523
FspBI CTAG 2 cut(s) 47, 279
GluI GCNGC 2 cut(s) 150, 523
GsaI CCCAGC 2 cut(s) 38, 678
HaeIII GGCC 1 cut(s) 42
HgaI GACGC 3 cut(s) 205, 207, 291
Hin1I GRCGYC 2 cut(s) 218, 648
Hin1II CATG 4 cut(s) 55, 157, 433, 634
HinfI GANTC 2 cut(s) 84, 164
HphI GGTGA 2 cut(s) 453, 470
Hpy166II GTNNAC 2 cut(s) 222, 514
Hpy188I TCNGA 4 cut(s) 203, 333, 471, 548
Hpy188III TCNNGA 1 cut(s) 451
Hpy8I GTNNAC 2 cut(s) 222, 514
Hpy99I CGWCG 1 cut(s) 241
HpyAV CCTTC 1 cut(s) 457
HpyCH4III ACNGT 3 cut(s) 109, 419, 511
HpyCH4IV ACGT 3 cut(s) 441, 498, 648
HpyCH4V TGCA 3 cut(s) 126, 193, 400
HpyF10VI GCNNNNNNNGC 4 cut(s) 123, 132, 239, 472
HpyF3I CTNAG 2 cut(s) 251, 545
HpySE526I ACGT 3 cut(s) 441, 498, 648
Hsp92I GRCGYC 2 cut(s) 218, 648
Hsp92II CATG 4 cut(s) 55, 157, 433, 634
Ksp22I TGATCA 1 cut(s) 435
Kzo9I GATC 4 cut(s) 292, 345, 435, 582
LmnI GCTCC 2 cut(s) 78, 204
LpnPI CCDG 8 cut(s) 24, 48, 55, 127, 350, 517, 660, 671
Lsp1109I GCAGC 1 cut(s) 534
LweI GCATC 5 cut(s) 126, 135, 475, 484, 708
MaeI CTAG 2 cut(s) 47, 279
MaeII ACGT 3 cut(s) 441, 498, 648
MaeIII GTNAC 3 cut(s) 459, 625, 649
MalI GATC 4 cut(s) 294, 347, 437, 584
MboI GATC 4 cut(s) 292, 345, 435, 582
MboII GAAGA 2 cut(s) 466, 565
MflI RGATCY 1 cut(s) 582
MhlI GDGCHC 1 cut(s) 447
MlyI GAGTC 1 cut(s) 158
MnlI CCTC 8 cut(s) 53, 86, 154, 244, 512, 579, 682, 703
MslI CAYNNNNRTG 3 cut(s) 266, 365, 599
MvnI CGCG 1 cut(s) 302
MwoI GCNNNNNNNGC 4 cut(s) 123, 132, 239, 472
NdeII GATC 4 cut(s) 292, 345, 435, 582
NlaIII CATG 4 cut(s) 55, 157, 433, 634
NlaIV GGNNCC 3 cut(s) 33, 74, 592
NmuCI GTSAC 2 cut(s) 459, 649
NspI RCATGY 1 cut(s) 433
PaeI GCATGC 1 cut(s) 433
PceI AGGCCT 1 cut(s) 42
PfeI GAWTC 1 cut(s) 84
PkrI GCNGC 2 cut(s) 151, 524
PleI GAGTC 1 cut(s) 158
PpsI GAGTC 1 cut(s) 158
Ppu21I YACGTR 1 cut(s) 499
PshAI GACNNNNGTC 1 cut(s) 327
PspEI GGTNACC 1 cut(s) 625
PspFI CCCAGC 2 cut(s) 34, 674
PspN4I GGNNCC 3 cut(s) 33, 74, 592
PspPI GGNCC 2 cut(s) 31, 590
PsuI RGATCY 1 cut(s) 582
RsaI GTAC 3 cut(s) 353, 497, 638
RsaNI GTAC 3 cut(s) 352, 496, 637
RseI CAYNNNNRTG 3 cut(s) 266, 365, 599
SatI GCNGC 2 cut(s) 150, 523
Sau3AI GATC 4 cut(s) 292, 345, 435, 582
Sau96I GGNCC 2 cut(s) 31, 590
ScaI AGTACT 1 cut(s) 353
SchI GAGTC 1 cut(s) 158
SduI GDGCHC 1 cut(s) 447
SetI ASST 7 cut(s) 257, 444, 501, 527, 553, 651, 714
SfaNI GCATC 5 cut(s) 126, 135, 475, 484, 708
SinI GGWCC 2 cut(s) 31, 590
SmiMI CAYNNNNRTG 3 cut(s) 266, 365, 599
SphI GCATGC 1 cut(s) 433
SseBI AGGCCT 1 cut(s) 42
SsiI CCGC 6 cut(s) 150, 188, 282, 300, 464, 503
SspMI CTAG 2 cut(s) 47, 279
StuI AGGCCT 1 cut(s) 42
TaaI ACNGT 3 cut(s) 109, 419, 511
TaiI ACGT 3 cut(s) 444, 501, 651
TaqI TCGA 4 cut(s) 236, 295, 344, 708
TatI WGTACW 2 cut(s) 351, 636
TauI GCSGC 1 cut(s) 152
TfiI GAWTC 1 cut(s) 84
TscAI CASTG 1 cut(s) 514
TseFI GTSAC 2 cut(s) 459, 649
TseI GCWGC 1 cut(s) 522
Tsp45I GTSAC 2 cut(s) 459, 649
TspRI CASTG 1 cut(s) 514
VpaK11BI GGWCC 2 cut(s) 31, 590
XceI RCATGY 1 cut(s) 433
XmiI GTMKAC 1 cut(s) 221
XspI CTAG 2 cut(s) 47, 279
ZraI GACGTC 1 cut(s) 649
ZrmI AGTACT 1 cut(s) 353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.